LOCUS       DAZSNG010000001        95093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZSNG010000001 DAZSNG010000000
VERSION     DAZSNG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95093
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.001"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(90..1340)
                     /gene="icd"
                     /locus_tag="ACHW4Z_000001"
     CDS             complement(90..1340)
                     /gene="icd"
                     /locus_tag="ACHW4Z_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR8879830.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1512..2165
                     /gene="rluE"
                     /locus_tag="ACHW4Z_000002"
     CDS             1512..2165
                     /gene="rluE"
                     /locus_tag="ACHW4Z_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR8879831.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2175..2636
                     /gene="nudJ"
                     /locus_tag="ACHW4Z_000003"
     CDS             2175..2636
                     /gene="nudJ"
                     /locus_tag="ACHW4Z_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR8879832.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2690..3796
                     /gene="mnmA"
                     /locus_tag="ACHW4Z_000004"
                     /gene_synonym="trmU"
     CDS             2690..3796
                     /gene="mnmA"
                     /locus_tag="ACHW4Z_000004"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR8879833.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3832..4473
                     /gene="hflD"
                     /locus_tag="ACHW4Z_000005"
     CDS             3832..4473
                     /gene="hflD"
                     /locus_tag="ACHW4Z_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR8879834.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4477..5847
                     /gene="purB"
                     /locus_tag="ACHW4Z_000006"
     CDS             4477..5847
                     /gene="purB"
                     /locus_tag="ACHW4Z_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR8879835.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6016..6687
                     /gene="phoP"
                     /locus_tag="ACHW4Z_000007"
     CDS             6016..6687
                     /gene="phoP"
                     /locus_tag="ACHW4Z_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR8879836.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6687..8147
                     /gene="phoQ"
                     /locus_tag="ACHW4Z_000008"
     CDS             6687..8147
                     /gene="phoQ"
                     /locus_tag="ACHW4Z_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR8879837.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     SWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8944..9225)
                     /locus_tag="ACHW4Z_000009"
     CDS             complement(8944..9225)
                     /locus_tag="ACHW4Z_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879838.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9239..10900)
                     /locus_tag="ACHW4Z_000010"
     CDS             complement(9239..10900)
                     /locus_tag="ACHW4Z_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR8879839.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10884..11210)
                     /locus_tag="ACHW4Z_000011"
     CDS             complement(10884..11210)
                     /locus_tag="ACHW4Z_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR8879840.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11266..11448)
                     /locus_tag="ACHW4Z_000012"
     CDS             complement(11266..11448)
                     /locus_tag="ACHW4Z_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879841.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11432..11872)
                     /locus_tag="ACHW4Z_000013"
     CDS             complement(11432..11872)
                     /locus_tag="ACHW4Z_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8879842.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11872..12168)
                     /locus_tag="ACHW4Z_000014"
     CDS             complement(11872..12168)
                     /locus_tag="ACHW4Z_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR8879843.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12165..12503)
                     /locus_tag="ACHW4Z_000015"
     CDS             complement(12165..12503)
                     /locus_tag="ACHW4Z_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR8879844.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12500..13711)
                     /locus_tag="ACHW4Z_000016"
     CDS             complement(12500..13711)
                     /locus_tag="ACHW4Z_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR8879845.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13713..14285)
                     /locus_tag="ACHW4Z_000017"
     CDS             complement(13713..14285)
                     /locus_tag="ACHW4Z_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR8879846.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14325..15482)
                     /locus_tag="ACHW4Z_000018"
                     /pseudo
     CDS             complement(14325..15482)
                     /locus_tag="ACHW4Z_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15873..15947)
                     /locus_tag="ACHW4Z_000019"
                     /pseudo
     CDS             complement(15873..15947)
                     /locus_tag="ACHW4Z_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15957..16262)
                     /locus_tag="ACHW4Z_000020"
     CDS             complement(15957..16262)
                     /locus_tag="ACHW4Z_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879847.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16259..16537)
                     /locus_tag="ACHW4Z_000021"
     CDS             complement(16259..16537)
                     /locus_tag="ACHW4Z_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879848.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16826..18574)
                     /locus_tag="ACHW4Z_000022"
     CDS             complement(16826..18574)
                     /locus_tag="ACHW4Z_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR8879849.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18571..18870)
                     /locus_tag="ACHW4Z_000023"
     CDS             complement(18571..18870)
                     /locus_tag="ACHW4Z_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8879850.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18876..19109)
                     /locus_tag="ACHW4Z_000024"
     CDS             complement(18876..19109)
                     /locus_tag="ACHW4Z_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879851.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19111..19331)
                     /locus_tag="ACHW4Z_000025"
                     /pseudo
     CDS             complement(19111..19331)
                     /locus_tag="ACHW4Z_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19324..19545)
                     /locus_tag="ACHW4Z_000026"
     CDS             complement(19324..19545)
                     /locus_tag="ACHW4Z_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879852.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19535..19735)
                     /locus_tag="ACHW4Z_000027"
     CDS             complement(19535..19735)
                     /locus_tag="ACHW4Z_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR8879853.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19865..20044
                     /locus_tag="ACHW4Z_000028"
     CDS             19865..20044
                     /locus_tag="ACHW4Z_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879854.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20169..20498
                     /locus_tag="ACHW4Z_000029"
     CDS             20169..20498
                     /locus_tag="ACHW4Z_000029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879855.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20548..20736)
                     /locus_tag="ACHW4Z_000030"
     CDS             complement(20548..20736)
                     /locus_tag="ACHW4Z_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8879856.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21111..22340)
                     /locus_tag="ACHW4Z_000031"
     CDS             complement(21111..22340)
                     /locus_tag="ACHW4Z_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8879857.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22589..23710
                     /gene="roxA"
                     /locus_tag="ACHW4Z_000032"
                     /gene_synonym="ycfD"
     CDS             22589..23710
                     /gene="roxA"
                     /locus_tag="ACHW4Z_000032"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR8879858.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23759..24985)
                     /gene="pepT"
                     /locus_tag="ACHW4Z_000033"
     CDS             complement(23759..24985)
                     /gene="pepT"
                     /locus_tag="ACHW4Z_000033"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR8879859.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25235..26371
                     /gene="potA"
                     /locus_tag="ACHW4Z_000034"
     CDS             25235..26371
                     /gene="potA"
                     /locus_tag="ACHW4Z_000034"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR8879860.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26355..27212
                     /gene="potB"
                     /locus_tag="ACHW4Z_000035"
     CDS             26355..27212
                     /gene="potB"
                     /locus_tag="ACHW4Z_000035"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR8879861.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27209..28003
                     /gene="potC"
                     /locus_tag="ACHW4Z_000036"
     CDS             27209..28003
                     /gene="potC"
                     /locus_tag="ACHW4Z_000036"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR8879862.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28000..29046
                     /gene="potD"
                     /locus_tag="ACHW4Z_000037"
     CDS             28000..29046
                     /gene="potD"
                     /locus_tag="ACHW4Z_000037"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR8879863.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29202..30023)
                     /gene="cobB"
                     /locus_tag="ACHW4Z_000038"
     CDS             complement(29202..30023)
                     /gene="cobB"
                     /locus_tag="ACHW4Z_000038"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR8879864.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30039..30950)
                     /gene="nagK"
                     /locus_tag="ACHW4Z_000039"
     CDS             complement(30039..30950)
                     /gene="nagK"
                     /locus_tag="ACHW4Z_000039"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR8879865.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30979..32223)
                     /gene="lolE"
                     /locus_tag="ACHW4Z_000040"
     CDS             complement(30979..32223)
                     /gene="lolE"
                     /locus_tag="ACHW4Z_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR8879866.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32223..32924)
                     /gene="lolD"
                     /locus_tag="ACHW4Z_000041"
     CDS             complement(32223..32924)
                     /gene="lolD"
                     /locus_tag="ACHW4Z_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR8879867.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32917..34116)
                     /gene="lolC"
                     /locus_tag="ACHW4Z_000042"
     CDS             complement(32917..34116)
                     /gene="lolC"
                     /locus_tag="ACHW4Z_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR8879868.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34378..35451
                     /locus_tag="ACHW4Z_000043"
     CDS             34378..35451
                     /locus_tag="ACHW4Z_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8879869.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35579..39025
                     /gene="mfd"
                     /locus_tag="ACHW4Z_000044"
     CDS             35579..39025
                     /gene="mfd"
                     /locus_tag="ACHW4Z_000044"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR8879870.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39168..40130
                     /gene="ldtC"
                     /locus_tag="ACHW4Z_000045"
     CDS             39168..40130
                     /gene="ldtC"
                     /locus_tag="ACHW4Z_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR8879871.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40213..40425)
                     /locus_tag="ACHW4Z_000046"
     CDS             complement(40213..40425)
                     /locus_tag="ACHW4Z_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR8879872.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40710..41342
                     /gene="comR"
                     /locus_tag="ACHW4Z_000047"
     CDS             40710..41342
                     /gene="comR"
                     /locus_tag="ACHW4Z_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR8879873.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41404..41943)
                     /locus_tag="ACHW4Z_000048"
     CDS             complement(41404..41943)
                     /locus_tag="ACHW4Z_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8879874.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42170..43474)
                     /gene="ndh"
                     /locus_tag="ACHW4Z_000049"
     CDS             complement(42170..43474)
                     /gene="ndh"
                     /locus_tag="ACHW4Z_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR8879875.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43882..44424)
                     /gene="ycfP"
                     /locus_tag="ACHW4Z_000050"
     CDS             complement(43882..44424)
                     /gene="ycfP"
                     /locus_tag="ACHW4Z_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR8879876.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44447..45472)
                     /gene="nagZ"
                     /locus_tag="ACHW4Z_000051"
     CDS             complement(44447..45472)
                     /gene="nagZ"
                     /locus_tag="ACHW4Z_000051"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR8879877.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45483..46307)
                     /gene="thiK"
                     /locus_tag="ACHW4Z_000052"
     CDS             complement(45483..46307)
                     /gene="thiK"
                     /locus_tag="ACHW4Z_000052"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR8879878.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46288..46929)
                     /gene="lpoB"
                     /locus_tag="ACHW4Z_000053"
     CDS             complement(46288..46929)
                     /gene="lpoB"
                     /locus_tag="ACHW4Z_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR8879879.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46944..47321)
                     /locus_tag="ACHW4Z_000054"
     CDS             complement(46944..47321)
                     /locus_tag="ACHW4Z_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR8879880.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47324..47683)
                     /gene="hinT"
                     /locus_tag="ACHW4Z_000055"
     CDS             complement(47324..47683)
                     /gene="hinT"
                     /locus_tag="ACHW4Z_000055"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR8879881.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48016..50201
                     /gene="fhuE"
                     /locus_tag="ACHW4Z_000056"
                     /pseudo
     CDS             48016..50201
                     /gene="fhuE"
                     /locus_tag="ACHW4Z_000056"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50261..51694)
                     /gene="ptsG"
                     /locus_tag="ACHW4Z_000057"
     CDS             complement(50261..51694)
                     /gene="ptsG"
                     /locus_tag="ACHW4Z_000057"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR8879882.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51989..52786)
                     /locus_tag="ACHW4Z_000058"
     CDS             complement(51989..52786)
                     /locus_tag="ACHW4Z_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8879883.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52797..53801)
                     /gene="holB"
                     /locus_tag="ACHW4Z_000059"
     CDS             complement(52797..53801)
                     /gene="holB"
                     /locus_tag="ACHW4Z_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR8879884.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53798..54439)
                     /gene="tmk"
                     /locus_tag="ACHW4Z_000060"
     CDS             complement(53798..54439)
                     /gene="tmk"
                     /locus_tag="ACHW4Z_000060"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8879885.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54429..55451)
                     /gene="yceG"
                     /locus_tag="ACHW4Z_000061"
     CDS             complement(54429..55451)
                     /gene="yceG"
                     /locus_tag="ACHW4Z_000061"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR8879886.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55454..56263)
                     /gene="pabC"
                     /locus_tag="ACHW4Z_000062"
     CDS             complement(55454..56263)
                     /gene="pabC"
                     /locus_tag="ACHW4Z_000062"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR8879887.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56383..57624)
                     /gene="fabF"
                     /locus_tag="ACHW4Z_000063"
     CDS             complement(56383..57624)
                     /gene="fabF"
                     /locus_tag="ACHW4Z_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR8879888.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57711..57947)
                     /gene="acpP"
                     /locus_tag="ACHW4Z_000064"
     CDS             complement(57711..57947)
                     /gene="acpP"
                     /locus_tag="ACHW4Z_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8879889.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58158..58892)
                     /gene="fabG"
                     /locus_tag="ACHW4Z_000065"
     CDS             complement(58158..58892)
                     /gene="fabG"
                     /locus_tag="ACHW4Z_000065"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR8879890.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58905..59834)
                     /gene="fabD"
                     /locus_tag="ACHW4Z_000066"
     CDS             complement(58905..59834)
                     /gene="fabD"
                     /locus_tag="ACHW4Z_000066"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR8879891.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59850..60803)
                     /gene="fabH"
                     /locus_tag="ACHW4Z_000067"
     CDS             complement(59850..60803)
                     /gene="fabH"
                     /locus_tag="ACHW4Z_000067"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR8879892.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60871..61941)
                     /gene="plsX"
                     /locus_tag="ACHW4Z_000068"
     CDS             complement(60871..61941)
                     /gene="plsX"
                     /locus_tag="ACHW4Z_000068"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR8879893.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62022..62195)
                     /gene="rpmF"
                     /locus_tag="ACHW4Z_000069"
     CDS             complement(62022..62195)
                     /gene="rpmF"
                     /locus_tag="ACHW4Z_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR8879894.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62247..62768)
                     /gene="yceD"
                     /locus_tag="ACHW4Z_000070"
     CDS             complement(62247..62768)
                     /gene="yceD"
                     /locus_tag="ACHW4Z_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR8879895.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62967..63551
                     /gene="yceF"
                     /locus_tag="ACHW4Z_000071"
     CDS             62967..63551
                     /gene="yceF"
                     /locus_tag="ACHW4Z_000071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR8879896.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63663..64622)
                     /gene="rluC"
                     /locus_tag="ACHW4Z_000072"
     CDS             complement(63663..64622)
                     /gene="rluC"
                     /locus_tag="ACHW4Z_000072"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR8879897.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65195..68386
                     /gene="rne"
                     /locus_tag="ACHW4Z_000073"
     CDS             65195..68386
                     /gene="rne"
                     /locus_tag="ACHW4Z_000073"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR8879898.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68583..69525)
                     /gene="flgL"
                     /locus_tag="ACHW4Z_000074"
                     /pseudo
     CDS             complement(68583..69525)
                     /gene="flgL"
                     /locus_tag="ACHW4Z_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69537..71179)
                     /gene="flgK"
                     /locus_tag="ACHW4Z_000075"
                     /pseudo
     CDS             complement(69537..71179)
                     /gene="flgK"
                     /locus_tag="ACHW4Z_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71245..72186)
                     /gene="flgJ"
                     /locus_tag="ACHW4Z_000076"
     CDS             complement(71245..72186)
                     /gene="flgJ"
                     /locus_tag="ACHW4Z_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR8879899.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72186..73283)
                     /gene="flgI"
                     /locus_tag="ACHW4Z_000077"
     CDS             complement(72186..73283)
                     /gene="flgI"
                     /locus_tag="ACHW4Z_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR8879900.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73295..73993)
                     /gene="flgH"
                     /locus_tag="ACHW4Z_000078"
     CDS             complement(73295..73993)
                     /gene="flgH"
                     /locus_tag="ACHW4Z_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR8879901.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74046..74828)
                     /gene="flgG"
                     /locus_tag="ACHW4Z_000079"
     CDS             complement(74046..74828)
                     /gene="flgG"
                     /locus_tag="ACHW4Z_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR8879902.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74966..75553)
                     /gene="flgF"
                     /locus_tag="ACHW4Z_000080"
                     /pseudo
     CDS             complement(74966..75553)
                     /gene="flgF"
                     /locus_tag="ACHW4Z_000080"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(75655..75717)
                     /locus_tag="ACHW4Z_000081"
                     /pseudo
     CDS             complement(75655..75717)
                     /locus_tag="ACHW4Z_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(75737..76876)
                     /locus_tag="ACHW4Z_000082"
     CDS             complement(75737..76876)
                     /locus_tag="ACHW4Z_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR8879903.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76901..77596)
                     /gene="flgD"
                     /locus_tag="ACHW4Z_000083"
     CDS             complement(76901..77596)
                     /gene="flgD"
                     /locus_tag="ACHW4Z_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR8879904.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77608..78011)
                     /gene="flgC"
                     /locus_tag="ACHW4Z_000084"
                     /pseudo
     CDS             complement(77608..78011)
                     /gene="flgC"
                     /locus_tag="ACHW4Z_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78015..78431)
                     /gene="flgB"
                     /locus_tag="ACHW4Z_000085"
     CDS             complement(78015..78431)
                     /gene="flgB"
                     /locus_tag="ACHW4Z_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR8879905.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78586..79245
                     /gene="flgA"
                     /locus_tag="ACHW4Z_000086"
     CDS             78586..79245
                     /gene="flgA"
                     /locus_tag="ACHW4Z_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR8879906.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79321..79614
                     /gene="flgM"
                     /locus_tag="ACHW4Z_000087"
     CDS             79321..79614
                     /gene="flgM"
                     /locus_tag="ACHW4Z_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR8879907.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79619..80035
                     /gene="flgN"
                     /locus_tag="ACHW4Z_000088"
     CDS             79619..80035
                     /gene="flgN"
                     /locus_tag="ACHW4Z_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR8879908.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80075..81610)
                     /gene="murJ"
                     /locus_tag="ACHW4Z_000089"
     CDS             complement(80075..81610)
                     /gene="murJ"
                     /locus_tag="ACHW4Z_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR8879909.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81777..82643)
                     /locus_tag="ACHW4Z_000090"
                     /pseudo
     CDS             complement(81777..82643)
                     /locus_tag="ACHW4Z_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(82645..83292)
                     /locus_tag="ACHW4Z_000091"
     CDS             complement(82645..83292)
                     /locus_tag="ACHW4Z_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR8879910.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83303..83887)
                     /gene="rimJ"
                     /locus_tag="ACHW4Z_000092"
     CDS             complement(83303..83887)
                     /gene="rimJ"
                     /locus_tag="ACHW4Z_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8879911.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84123..85331
                     /gene="mdtH"
                     /locus_tag="ACHW4Z_000093"
     CDS             84123..85331
                     /gene="mdtH"
                     /locus_tag="ACHW4Z_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR8879912.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85395..86042
                     /gene="grxB"
                     /locus_tag="ACHW4Z_000094"
     CDS             85395..86042
                     /gene="grxB"
                     /locus_tag="ACHW4Z_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR8879913.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86176..86736
                     /locus_tag="ACHW4Z_000095"
     CDS             86176..86736
                     /locus_tag="ACHW4Z_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8879914.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86842..87888
                     /gene="pyrC"
                     /locus_tag="ACHW4Z_000096"
     CDS             86842..87888
                     /gene="pyrC"
                     /locus_tag="ACHW4Z_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR8879915.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87962..88207
                     /gene="dinI"
                     /locus_tag="ACHW4Z_000097"
     CDS             87962..88207
                     /gene="dinI"
                     /locus_tag="ACHW4Z_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR8879916.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88497..88751
                     /gene="bssS"
                     /locus_tag="ACHW4Z_000098"
     CDS             88497..88751
                     /gene="bssS"
                     /locus_tag="ACHW4Z_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR8879917.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88866..89984
                     /gene="solA"
                     /locus_tag="ACHW4Z_000099"
     CDS             88866..89984
                     /gene="solA"
                     /locus_tag="ACHW4Z_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR8879918.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90032..90145
                     /locus_tag="ACHW4Z_000100"
     CDS             90032..90145
                     /locus_tag="ACHW4Z_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR8879919.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90406..90972
                     /locus_tag="ACHW4Z_000101"
     CDS             90406..90972
                     /locus_tag="ACHW4Z_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR8879920.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90976..91551
                     /locus_tag="ACHW4Z_000102"
     CDS             90976..91551
                     /locus_tag="ACHW4Z_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR8879921.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91593..92645)
                     /locus_tag="ACHW4Z_000103"
     CDS             complement(91593..92645)
                     /locus_tag="ACHW4Z_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR8879922.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92870..93790
                     /gene="lpxL"
                     /locus_tag="ACHW4Z_000104"
     CDS             92870..93790
                     /gene="lpxL"
                     /locus_tag="ACHW4Z_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR8879923.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93962..94147
                     /locus_tag="ACHW4Z_000105"
                     /pseudo
     CDS             93962..94147
                     /locus_tag="ACHW4Z_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            94183..>95093
                     /locus_tag="ACHW4Z_000106"
     CDS             94183..>95093
                     /locus_tag="ACHW4Z_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8879924.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        22885 a        25211 c        23473 g        23524 t
ORIGIN      
        1 aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc gtgttatacg
       61 cccgtttaaa tatttaacac atgtagtgat tacatattct tgatgatcgc atcaccaaac
      121 tctgaacatt tcagcagttt agcgccttcc atcagacgct cgaagtcata ggttacggtc
      181 ttggcgttga tcgcgccttc cataccttta acaatcaggt ctgcggcttc agtccagccc
      241 atatggcgta acatcatctc agcggagaga ataatagagc cagggtttac tttgtcctga
      301 ccggcgtatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg
      361 tttgctccag gggcgatacc gataccgcca acctgcgctg ccagagcgtc agaaatgtag
      421 tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt
      481 tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt attcgggttt
      541 ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaattcttc acgcgccagc
      601 tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc
      661 agggtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt
      721 ttggtgcctt cttcagaaca cggcttaata ccgataccgc aatgttccgg gaagcgaatt
      781 ttcttcacgc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc ggcagagtca
      841 gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg
      901 gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg
      961 cagatgtaga gatccagttc ctgacgcagg gcaacgttga gagaacgaat accgccacca
     1021 accggagtgg tcagcgggcc tttaatggca acgcgatatt cacgaatcag atcaagggtt
     1081 tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt
     1141 tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact
     1201 ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta agggataatc
     1261 ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga
     1321 acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctgcca
     1381 ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa
     1441 tgatttgtaa ttgacttgtc aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt
     1501 attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta
     1561 gaaatcacca ggttaagcga ttcagctcgc aacgttctac caggcgtaaa catgaaaacc
     1621 agcccacgcg tgtgatcctc ttcaataaac cctacgatgt tcttccgcag ttcaccgatg
     1681 aaaccggacg caaaacatta aaagaattca tcccggttca gggcgtttat gcagcaggtc
     1741 gccttgaccg cgatagcgaa gggttgctgg tgctgaccaa taacggcgcg ttgcaggcgc
     1801 gtttaaccca gccgggtaaa cgcaccggaa aaatctatta tgtgcaggtg gaaggtattc
     1861 ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat ggccctaccc
     1921 tgcccgcagg cgcggagctg gttgacgaac ccgcgtggtt atggccgcgg aatccaccaa
     1981 ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta
     2041 atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgct
     2101 atgcgatggg agattactct ttggataatc ttgccaatgg cgaatggcga gaagtgacag
     2161 attaaggatg caaaatgttt aaaccgcacg ttaccgttgc ttgcgtggtg cacgcagaag
     2221 gcaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac caacctgccg
     2281 ggcatctgga agccgatgaa accttagtgg aagccgccgc ccgtgaactg tgggaagaaa
     2341 ccggcatcag cgcgcagccg caacacttta ttcgtatgca tcagtggatt gcgccagata
     2401 aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacccagc
     2461 ctcatgacag cgatatcgac tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt
     2521 caaatcttcg ttcgccgctg gtggcggaaa gtattcgttg ttatcaaagc gggcaacgtt
     2581 atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg
     2641 ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac
     2701 cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct
     2761 gttgcaacaa cagggatatc aggtcgaagg cctgtttatg aagaactggg aagaagacga
     2821 cggtgaggaa tattgcacag cagcagcgga tctggctgat gcccaggcgg tctgcgacaa
     2881 gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca acgtcttcga
     2941 actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc tgtgcaacaa
     3001 ggagatcaaa tttaaagcct tcctcgaatt tgccgccgaa gatttaggtg ccgattatat
     3061 cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc tgctgcgtgg
     3121 tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg agcagattgc
     3181 gcaaagcctg ttcccggtag gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga
     3241 tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca ttggcgaacg
     3301 taaattccgc gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt
     3361 cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg gtcagcgtaa
     3421 aggtctgggt atcggtggca ccaaagaagg taccgaagaa ccgtggtatg tggtggacaa
     3481 agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc ggctgatgtc
     3541 tgtcgggttg attgcccagc agctgcactg ggtcgatcgc gaaccattca ccggcactat
     3601 gcgttgcacg gtaaaaaccc gctatcgcca gaccgacatt ccgtgcaccg tcaaggcgct
     3661 ggacgacgat cgcattaaag tgattttcga tgagccggtt gccgccgtga cgccgggcca
     3721 gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct
     3781 gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag
     3841 aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa
     3901 caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact caacagtatt
     3961 attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc caacctgcgc
     4021 gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gttgccaggg cttaaacgcc
     4081 gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc ctcagcgaaa
     4141 ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc
     4201 gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga tgtgatcagc
     4261 ccgcttggcc cgcgcattca ggtcaccggt tcccctgctg tactgcaaag cccacaagtg
     4321 caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag
     4381 gtcggcggcg gacgtctaca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa
     4441 caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc
     4501 gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc
     4561 gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc
     4621 gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat
     4681 gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat cgagcgtacc
     4741 actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg
     4801 gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga tatcaataac
     4861 ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt
     4921 caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc gctgctgtct
     4981 cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc aaacgtcgcc
     5041 taccgtatgg agcgccagca ccgccagctt aaccaggtgg agatcctcgg caaaatcaac
     5101 ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga ctggcatcag
     5161 ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac tacccagatt
     5221 gaaccgcacg actacattgc cgaactgttt gattgcgttg cgcgcttcaa caccattctg
     5281 atcgactttg accgtgacgt ctggggttat atcgccctta accacttcaa acagaaaacc
     5341 attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgaa
     5401 aactccgaag ggaatctggg cctttccaac gcggtattgc agcatctggc aagcaaactg
     5461 ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg
     5521 ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag caaactggaa
     5581 gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct ggcagaacca
     5641 atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctt
     5701 actcgcggta agcgcgttga cgccgaaggc atgaagcagt ttatcgatgg tctggcgttg
     5761 ccagaagaag agaaagcccg cctgaaagcg atgacgccgg ctaactatat tggtcgagct
     5821 atcacgatgg ttgatgagct gaaataagcc tcaaatcagt gccggatggc gatgctgtcc
     5881 ggcctgctta ttaagattat ccactttttc ttttttcact ttacctcccc tccccgctgg
     5941 tttatttaat gtttaccccc ataaacacat aatcgcgtta cactatttta ataattaaga
     6001 cagggagaaa taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac
     6061 cttaaagttc agattcagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa
     6121 gccgattatt atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcct
     6181 gacgaggacg gtctgtcact gattcgccgc tggcgtagca acgatgtttc actgccgatt
     6241 ctggtattaa ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgccggtgct
     6301 gatgattacg tgactaaacc gtttcatatt gaagaggtga tggcgcgaat gcaggcatta
     6361 atgcggcgta atagcggtct ggcttcacag gtcatttcgc tccccccgtt tcaggttgat
     6421 ctctctcgcc gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac
     6481 accattatgg aaacgttgat acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg
     6541 ctacaactct atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga
     6601 cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag tcattaccac cgttcgcggc
     6661 cagggctacc tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct
     6721 cgctgcgggt acgttttctg ttggcaacgg cagcggtagt actggtgctt tcgcttgcct
     6781 acggaatggt cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt
     6841 tacgtggcga gagcaatctg ttctataccc ttgcgcagtg ggaaaacaat aagttgcatg
     6901 tcgagttacc cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga
     6961 acgggcagct tttatgggcg caacgtgacg tgtcctggct gatgaaaatg atccagcctg
     7021 actggctgaa atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct
     7081 tgctgagtgg agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg
     7141 acgcggagat gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa
     7201 aattaaccat tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct
     7261 ggagctggtt tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg
     7321 tcgccgcctg gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg
     7381 aagaacataa ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac
     7441 gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc
     7501 tcaccgacct gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt
     7561 ctctgcgtag tgaaaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca
     7621 gccgcatttc acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat
     7681 tgctcagccg cgagctgcat ccggtcgccc cactgctgga caatctcacc tcagcgctga
     7741 acaaagtgta tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct
     7801 ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta
     7861 aatattgcct cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg
     7921 tggtcgagga tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg
     7981 gtcaacgggt tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa
     8041 tcaccgagca atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga
     8101 tggaggtgat ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtcctttta
     8161 ccactacatc aagtcattga ttcgatcaaa caattttcat tgaaaaagtg ccaaaaatcg
     8221 caaattgact acaccattaa ctacaccgtt cggtgcactg tatgaaacaa cgtggaacaa
     8281 atagacacaa gaaatataca ggcgggtaat ctttccaggg ggaagcgcac caattcatga
     8341 ggggcgttaa tgtcgatatg gggatcccca taacggggct accggatttt ttttccggtt
     8401 aacctttaat caggctggtg ggttttacat acccttgatc accgtaatga tgatccagat
     8461 gtgcaccttt tccaccaatg gcggaaaagc tccaaccgta acgatgatcg ctatggttgc
     8521 ctcgatggtc tgcgtggtca ttatgaccgt tcagccagtg accttaatca gaatttttcg
     8581 ctaaaaaatg acgttggcca cgtcatccgg aaagcacaaa atcacgtcga tttttaatga
     8641 acggttaaac cgtctttttg tcacggtgtg tcactggttg tcactctccg tcacgattga
     8701 ggtggtggtc attctgcccg accgcgcatc tttgcgctat cgggaatatc aaacagttac
     8761 ctcgccgact tcctcaaatt gaggtttccg aaaatatcag agcgggttag cgattaagct
     8821 gacgagtaat taaactgcga aaatttcgta gtttctgaat atattccggc ctgctggtta
     8881 acagtcctca gatgtgaggg atgtagccag cgcaattttg cgctttgctt taatctcagt
     8941 gagttaccgc cgtaaccgct ccggcttctt ccagtggtac gtgattttct ccttctcccg
     9001 atacagttca acgcggcgat tgtaggccag catttccaga acgcggatcc gtatgtcgcg
     9061 catatccgca tcattaagct ggataccatc acggcgcatc acctcagcaa caacacgcgc
     9121 ataattttcg gctgtcacgc tgtccggctg cgtggtctgt tcgtcagcct gctggctgat
     9181 tccagagacg cggcggatta atcgcagtat ttcggcttct gtcatgctgc tgacctcatt
     9241 acaccccgct aaattcttcc agtttctggc ggtggctgtc gcttatatca aaagcaaaat
     9301 cctcatgctc tgcctggaat gtaccaaacg ccatcagcgc cgctacgctc gggtctatct
     9361 tgttcggtga ttttttcttg ttcggcttga tattggcgtt cgcgtcactc tgcatcacga
     9421 cgttactcat cgaccaggac aacaccggat cgccacgatg cacaatcacc ctgcggttaa
     9481 caaaaacttc gaacgatttc gccgccggac tgaatctgag gtaggtttgc gggaacggct
     9541 ccacctcaaa accagccccc tgtaattgcg tcctgagatg cgtggcgttc cacgtatcaa
     9601 agcccaccag cctgatatta aatttctccg catcctgcat gatgtcatct ctgatccggt
     9661 cgtaatcaat gcagtcacct ggcgttgtgc gtatccagcc cgctttagcc cactggcgat
     9721 agacagcgcg gtttttattg gcggggttct gtagctggaa ctccggcaga taatgacggg
     9781 aaatcagcat aatggtttta ccgaccggaa aggcatagca tacgctggaa atatcgctgg
     9841 ttgatgataa gtccagcccc gcgtaacact cctgaccgtg taaatcttcc tccgtgaacg
     9901 ttccggcaca ctcagcccat gcaccattac ccatccacgg ggtagtcccc tgacaccaga
     9961 tattaaatcg ctttgtcatc atctccaccc actgcgacgg aataccccgc gctttctgga
    10021 tggttgaggc cagtttttca cgatccacgg aaacatcaat attagggttc gcctttatcc
    10081 acatcgccgg atcatcaacc tcgttttcgt cgtccagttc gtaaatcagt acaaaaattg
    10141 aatcgttaac ctcttcgccg tccaggatct ggcagcaata atcatagtgc tgtttacagg
    10201 ctgaaacgac gttactgccc gatgtggtaa tggcaaataa cagcccttcg ggacgtgcgc
    10261 ccatccccag ctcaagcgcg gaataaacgc cgttatcggg gtgtaggtgg tattcgtcca
    10321 cgatggaaag actggggttt gtcccctcga tggttgccgc ttttgctgcc agcggcttta
    10381 acaggctgtt gcttttcggg tgtatcacct tatgcgcctg aatattcacc cgcctgcgta
    10441 acggtcggga taaaaggcac atctgacgcg cattatcaaa cacgatccgc gcctggtcac
    10501 ggctcaccgc tgcggtgtag atatcctgct gaccgttctc cataatcaga aaccagttag
    10561 ccagaatcgc ggcggtcgtg gatttcgcat ttttgcgcgg cacttcgata aaggcgctcg
    10621 tgtatttgcg ccgtccggtg gccttaacct taaagccgag gatgcacgca aaggcgaact
    10681 gctgccacgg ctccagctca atgggtctgc cacgcatcgg tccttttacg tgcgggcaca
    10741 ccctggaaaa ggcaataaac cgctccacaa cctcacgatc gaacgtgtaa agggggcttt
    10801 taaggtccga aaagtaccgt ttaacagcct gttttaaccg tttacaggcc ggaattttgc
    10861 ccgtttttac gtcttctgcg tacttattcc aggcggtcaa gctcgtcctc ttcttctgtt
    10921 tccggtggat ttttacggcg gcttatcggg tcaaaaccga gcaaggaggc catttttatc
    10981 atcactcttt cagcgtcgga ttttgcgctt aatgcggggt ttctgctctc gccgccctga
    11041 ctgttaacaa tgctgaaccc gcgcgccgca aggtctgcaa cggctttccg gtaaatggag
    11101 tagttgacgc aatacagttc cagattgctc cagtcggcgg gggtcaggtc tccccgttcc
    11161 gcaagctgcc gcgatttttc ccgccactgc ttcacggcga tatcatccag gtaggcgggg
    11221 tcgggtttgt cctgaaaacg ccccccaccc cgtcatacag cctcatcagc gattgcggaa
    11281 acattccata acctcgcggt cacggtcggt taatcgcttc gctgtgatgc gttctgcgcg
    11341 tcctgacgct ttatctttat gccctgtttc ctgtgtcttc catgcgtcac gctgccttat
    11401 aagtccacgg ataaggcggt tttgttcccg ctcattcatc agcgtcatac atccagttat
    11461 tgcggttagc agcccgttct tcctcttcac gaaatccacc tgcggcacgc ttgctttttg
    11521 ttgccggatc aagccatttc gttttctggt tatgacacgc ctggcacaat ggttgatggt
    11581 tccattcggg ccagaagaga acatcatcac cgccattaat cgggataatg tgatccacca
    11641 ccacggcggg cgtatatatc cccttctcaa ggcatcgcac gcataacggg tttttactca
    11701 gatacatggc gcggtatttg tcccactgtc tggaatatcc acgcgcgcgg cggtgtcctc
    11761 gtctggcatc ctctgcacgc catgccgccc gcctgtgctc ctcacacttg ccggatttaa
    11821 cgcgcctgtt acagcctggt tctgtgcatc gtcttaatgg ttgccacggc atcagtaccc
    11881 ccccacatca cggtaagccg tccagagtgc gcctatcgtc atgggtacgc gtgtttttgc
    11941 gttatccgcg acaatctggc gattttcata caggtgagcg ataaacatca tgcagccaat
    12001 ctttatggct ggcgtgaacg ccagcccgtc atcaaagcgc ctgcctatgt gtatctggca
    12061 cgcctcaagc gatgcggcaa tgtatccgct gattaactgg tcttcatcgt cgccatcgat
    12121 gcggcaatgg agtttcactt cttccagggt gataagttct tctgtcattt ttccgcgccc
    12181 tcacgacaaa gaatttcaag gcgtgtcctg gcggcatccg gcagcggctg cccgatgata
    12241 ttcagtacac gccccgccag cggccccgta ctgaccttta tccggctggt ggcgttgatg
    12301 tccttccggt accgtatcca gatccttacg gtaccaatcg ccagctctgc gcccgatgaa
    12361 atggcctcct tgctgctgac catgttcacg cttgcccaga gtgtgtgacc gtcctcccac
    12421 gtttcgagta tttcgccggt tatatccctg gtctgtttca gggtctgaat cgttatcctg
    12481 tcacgcagtc gccctatgtt cattccggtt tttctccctc gctgattttt acttcctgtt
    12541 tccatgcctg gctgaactca tcaccaccat cacgcggtga cagcccttca cgttcgcggg
    12601 cttcgttcgg acacataacg tcggatttta tgccgcgctc ataggtggca aagcgttcgc
    12661 caggtgtggc ccgtaacagg tccgcgctgt caaactccac ctgataccgg ataccaggta
    12721 caggcgaggc caccagcaag gcgtttttta tctgctgctc aaagttcgcc agccacgggc
    12781 gcatcgtcat ggtgagaaat gcgcggcttg cctcgctgaa gttgctgtag gtgctgttgc
    12841 tgtattcctg caaaaaaatc ggcgagacgt tgaacattcg ggcgatgtct tcaatggtga
    12901 agcgacggga ggccagccat tcggcatcct gattactcat gcccagctgc tgatagctca
    12961 tccccccttc aaggatgggc gtttttccgg cgtttctggc ccctttgtag cgttccagtg
    13021 cggctaatgc ctgtttgccc ttcacgccgt ccagccattc gcctgacgtg ataacccctg
    13081 ccgccatcat gccatcacgc atcacgctcg cgccgtggcg ttgttgggcc agcccaagcc
    13141 ccagcgattc gcggcagatg gttacaggtg agcgccccat aaaaccgtca tccgtggagt
    13201 aacgaaggtg gagaacctcc cacggtaaat agttgcggat gtttccggtg taggcgtcag
    13261 tgatgcagta gcgccagtta tgatcacctg tctgctccac gttcaccgac tgcggcgggt
    13321 aaggatgtaa agccgccgga aaaccatcac gcccccactg aataaccgca taagcattac
    13381 cacttaacag gcagtagcgg atcatcattc gcttaaactg gtagggtgtc tgccacgcgt
    13441 tcgggcgctc gttgaggatg tgatcgaccg gatgagaatc aagccattcg cgggcctcct
    13501 tccccttctc attgcgtacc aggtacaggt aacacggcat ggtagccacc gcgtcagaga
    13561 tgaccgtgac ggcgttcatg acggcgggga gtgattccgc tgtcccagat gatacgtact
    13621 cacccgcccc cgtgttcgat gtgccggcca gcgccataaa ttcatcaagc gtcatgctgc
    13681 gctgctcttt ttttcgtctg aaaggccaca tatcacaccc ccgctaaatc cgcccaccag
    13741 cggcgattat ccgcacgcgg cattttttcg gggtgctgct catacaggga acggcgggcc
    13801 agctccacgc cggaatcggg gtaagccggt accgatgtaa cggtaatttc gtacagttcc
    13861 gccaccagca cggtgcgcac gcatggatct gttgtggtat cccatacatc cttacgggag
    13921 cgaaagccaa agctcatccc cgagatgtcc ccacgcttaa ccagttcgat aacgtcgcgc
    13981 cctgtactgg tatccggtgg ggtcagttca aagcgtaacc ctgtttcgtc ctcttccagt
    14041 ttcagcgtgc cggaacgggt gcgccccagt aacatgctgt ggtcatgctc atacaggccg
    14101 cgaacgtcat tacccgccgc aagccattca gtaaacgccc cccgctggaa tttttcgtaa
    14161 aactcacccc ataacagttc tgaaagctta tcccagcgaa caacgtagcc cgtcagcgta
    14221 ccggctccgc tggtggtgat ttccgatgac cggatttcca tactcttcat aattttttca
    14281 cccataaaac actaaagggg ctttttagcc ccttctgtat gctgttaatc gtcgtcttcc
    14341 ggcagtgcca gaatcttgat cgcgttcgaa tccaccacgc caccgcctaa atatttttgc
    14401 gtgaaaattt tgatgaatcc tggttctgtc aggttgtccg gtctggtgcg aacacctgtt
    14461 tcgtgatcaa caatgtagta accgcgtttg aagtcaccca gggcaataac gttatcaggc
    14521 ataaactcca gatattcgac cggaaggccc agcaacgtat caggatcacc cgcctgtaaa
    14581 cggtcgcgcc agatataatc accgttcgcg ttcttcacct tctgaagttt tgccgccgtc
    14641 gtggagttaa ccactcagac cgcgtttttg cggtattttt tacgtaatgc aaatttcagg
    14701 tcgatcagcg ggtccgcaga tgtccacgcc agagattcgg aaggtttaat tacctgcaac
    14761 gtaccaaaat cacgctcttt gtcgttcttc tctgcacggg gtacggataa aaaacctttt
    14821 gcttttttgt caccgtcgcc cacaaccaga tcgctttctt cggtttccgt gaaggtgtcg
    14881 ccaatctcac ccgtcagcca tgaaaggatg tccacatcgg aaaaatccac gatttcctgt
    14941 gtggtgcgcg ggtacgcata gaccggatac agcttaatgc tcacctcgtt aatctgcggg
    15001 gtgctggtct gttcgcgtgc cttaccctct tcaccgtggt taacggtcgc accgccagcg
    15061 gaaacaagct gcttaaactc gttgctgctg attttcttca cggtacagat gcgacgcatg
    15121 gtggattcat ccgccagcat tcgcatgatt tcggtgttca gttcagggat aacggtataa
    15181 ccaccatcag cgggaacgcc tgtacttaat gcgcgggttt caccggtcag aatgtagtta
    15241 cgtagttctg cggggtcagt ggtctggctg cttttacctg gcttgctgcg ctcttcgtct
    15301 gcaatggctt caaggcggga aatgtcttta tcgagggatt cagctttagc gcgtaattcg
    15361 tcaaattttg cgccctcagc atcgttaaga ctgcggtttt ctttttcagc gttctccagc
    15421 atgtcgcgca tctgattttt aatggcggtt ttctgctggc gtaattcgat tattctctgc
    15481 ataaaaaaag tcctggggtt aagtaaggaa ctccaggacg cggcaaacac tcaaccgttt
    15541 ttcataagga aatcagtaat cgcaccgatc gttttcccgc ctggtaatga atattggcga
    15601 gcacattaac aggcgggaaa gtggccccag cgtcctggca ccacgggcga gaataatcat
    15661 gattcagttc gggtaaaata tgccgatccg ctcagtgaac aacgtggaac aaccacgaac
    15721 aaataattta caaaaaataa caaaaagccg gattgctccg gctgcgtggt gtggttctga
    15781 tattcctact ctgctaattt gctcagcccc atgtctgcgt aagtgcgatt tactgcattt
    15841 ctcaggtctg cgtagttctc cgttggcggc tccggtgtgc ccttgctgct ctgttttacc
    15901 agcgggtcgc ccaccagctg cccgtaagct gcaatttgtg ctgtcatatt gccacctcag
    15961 tgaagcgatc cggcattgtt ctttttcatc tcttccatca ttttttcagt gagcattacg
    16021 cactgaaacc ccatgttttt gatgtctacg ggtttcatgc ctttgtcagt ggatatcaca
    16081 ggcagaatat cctgatacac atcgcggtca gggtaactaa gcacacaaag ctcccatgat
    16141 gccacgggtg aggatccttc gtctgtttca acccaccagc ctggtgtagc tgcgcttatt
    16201 tgcagaatcg tgatccgtcc gttgtgcttg taaaattttt caccaaattt tggttcgctc
    16261 atgattccac ctctccggtt ttaacgttga ttgttgttac ctgttccgct tcggcaatct
    16321 cccgctctgt cagcgtggca aaatttgccg ccgtcgtggt catgaatgcg cttatcagtt
    16381 cgggatgtgc tttcgcgtat ccttcccccg cgtggcggtc tatcgttctg attgccacct
    16441 ttaaagcgtg ctctgtcatg tctaacgcgc gatatttcgg ttctgttctg tctctgcgtt
    16501 ttttgaatga tttattaaat ttccctgaag tgtgcatatt tatttttacc ccctcgttta
    16561 aaaagttttg agttgtgcct ccccttgtct accttatcta ccttagtggc cctcatgcca
    16621 gtaatggcgc tgctttcagc ggggtagagt gcttttatcc actatctacc ccgtgtctac
    16681 ctccctgtct gattcaggta aaatcaggta gagagggtag atagtgggta gacagtaaaa
    16741 aaaggctatc cacctaactt aatgcactga attaaatgta tttttcttta ctcaggtaga
    16801 cagggtagac agcaattaca aaaaattata aaaacgcgtc gcactcgtct gttgttattg
    16861 cgttagtctg cgttactccc ttaactttcc gcgtaatata ttcatgtccg taaactttcg
    16921 cggctggctt catagccttg ccaaagtcat ttacgtttag cggtttgctc ctgcctgcgt
    16981 acgccataaa cgccagatag acgcggtaaa ggctgtttct ggtcgtgtac ttcactgaat
    17041 cgccaccgcc gcccatcatc aggccgcgtg cttcctccag aaaattcagg aactggcaaa
    17101 actcaataac cggatccgtc tgttgcttta ttgccagagc ttcatcaccg tcacgctgtt
    17161 ccagtagtaa agcccgtgct ttctcagagt cggtaaagtt cgccagcaat cggcggataa
    17221 tgacagggat ttcagccgca atcttttccg gtagctccct gtctttttcg gcctcactaa
    17281 cgatattgtc gaaacggaaa atcacgcgac gacgtgccac acctccggcc cgttcggtga
    17341 atatcatcgg gttattgttg gtcgccagca ccaccgccct gattacagcc gtgaaacgct
    17401 tttcgtattt cgggttaatt tccacggggt caccgcccgt gattttcttg atgcccgttc
    17461 cttcgcctgt atatttcggc tggtcagcca ggacgataag acgactcccg acaacctgcg
    17521 cacgtccacc agcatcatca agcgatgtca tttcagcgct taccgtgttc tgtttccctg
    17581 ccagaaggct ggctatgtgt gtgaatgtac ttttaccgct cccgccgtct ccggtggcct
    17641 caataaacat ctgccagtcg taccggttcg ccataatcat gtacagcgcg gcacatatac
    17701 gcatcatctt gcgctggtct tttccggctg cgtgctcaag ccatttatga aagtttagcg
    17761 cgttatcgcg gatgttctcc cctggtgctg gtggcgtgta ctcaatgccg ttgtgcgtgg
    17821 tgatccagtt ctccggcgtg tgcggggaaa attcccccgt tttcaggtca agcgcaccat
    17881 tggcgaacgg cagcaaatcg ccggacggct cgcccattgg ttcggcaata acttttaacg
    17941 cttccacggc gttattgatt acgcgcttgc tgaaagtggc cctgtgctct gaatagatca
    18001 ccaccatttc gcggctaagt tccattgtgc tgaccggaca ccataccccg ccgcgccata
    18061 cgtgaacgat ttcactttcc tgatgcacac aaacgccatc aaagcgctcg gcaagcagct
    18121 gcgcgcgctc actgtctgcc atttgtgcca gttgcgtatt tttctgctcc ggcatggtaa
    18181 gccctgcggc aatattttca cgctcagcat tcagatagcg ccgccagttt tcacacttct
    18241 ggacgtgcat tccttcgggg taaaaatttg cgtcctgaat atctgccgcc gccagcttct
    18301 gaccaatctt tttggtctcc accaaatcca gttctccggc ctggtacagc cttacgcgtt
    18361 tttttccatc cggaacaatt ttcagtgcat caagttcggc aagctgattt ggcccaagcc
    18421 acacaggcgg cacattatcg ccggatgcgg ggccatcctg ctcctgccat tgtttcgcgt
    18481 gcgaccaggc atcactaccc gcaaagataa ttacctctgt gtctttgtgt tttattccgc
    18541 gtggctgttt ttttacgttc ggtgccagtt tcatttttta cccctgaata cgttaagcat
    18601 cttttttatt tcctgaatat tggcgcgtgc tttctccctg ctggttggtt tactgcgggg
    18661 cgctgcctgt accagcgtga aatcacgccg gaattgataa acaggcatca cgcaatcata
    18721 ttcgtaacct tcacggcggt aggtgatgcg ccgttctgcc acgcctttaa tcgttaccat
    18781 gccgccgtac tggtcgcggt aaatatcacc gcgcgtaaat ttagggtgag tgttgccact
    18841 ggcagttaag ccagaatatt taagtttcat tatttttatt ctccggtgtg ttgttcttta
    18901 tatctgtcgt gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt
    18961 attaatgccg tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca
    19021 agccatatgc gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg
    19081 tctattgttt cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat
    19141 aacctctgtc acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat
    19201 ttatattctc tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa
    19261 agcgcatcag ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga
    19321 tcatcacgca taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt
    19381 cagtgaccgc gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg
    19441 ttcttctccc tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt
    19501 agtgagtgcg gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac
    19561 gaatacgggc ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt
    19621 ccgtccaagc ggttacgtgg atgatttggg attctccggc actcagtgcc agaaaacgcc
    19681 acacaaaggc cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg
    19741 ctcctagttt acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc
    19801 tttttgtgtt gattgtgtgt aatgtgtcac tacacataaa tacattacct gaggtgtaat
    19861 taacgtgtca acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga
    19921 tcctgactta cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc
    19981 tgcctggata aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg
    20041 ataaaaacta tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac
    20101 aatgaccaac gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa
    20161 gaaagagcat gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa
    20221 agaatactca tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt
    20281 gcattgccca ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac
    20341 acaaacggcg cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt
    20401 tatgcctgaa aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg
    20461 cccacgttct ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg
    20521 tgtggcggct gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc
    20581 cagaacctcg gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa
    20641 ttgtccggcc ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca
    20701 ttctttttca cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa
    20761 gagttatttc gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt
    20821 tggcaaatgt tgaggccgca tggtttacag aaagaggaat aatcaggata aaatccttta
    20881 aattcatgtt aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa
    20941 gagtgattcg ctagtgtata aaaaatcagt aacacattaa aaatcagcta cttataaatc
    21001 tgtacacttt ttcacctctt gttcgtagtt gttccgcatt gttgctcatt gttgcacgtt
    21061 gtatctgttc gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt
    21121 ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc
    21181 gtccacatat ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc
    21241 tgcattcctg gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa
    21301 tcgttcagca tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt
    21361 agcctcctgt gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc
    21421 agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct
    21481 tccggtatgg gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag
    21541 agcatttcgg taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat
    21601 ccgaacacaa tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc
    21661 agtgcctgca ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt
    21721 tttttcccaa cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc
    21781 ctccggcaga acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc
    21841 gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc
    21901 ggcatagcac caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa
    21961 tccacgcggt tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt
    22021 accggcttta acgcttcctg tacggtgtaa ttaagctcat gacgtggatt tttcccctct
    22081 gccagccatg cgcgacactg ggcggctttt tccctggctg atttcaggct cagatcagga
    22141 taatttccca gcttaatgcg ttccggtggt gccccccttc ccgttccggc cctgtaagtg
    22201 aaataccagg ttaaaaggcc actggttgaa tgcctgacgc tcaggtttcc accgtcatta
    22261 agaaaggctg ttttttgggc gggtgtgccg ttgattttcc tcagctgtgt atcgctcagt
    22321 ttgttaagtg ctctgctcat aattttgatt ccggtcatca caattaacta caccattaac
    22381 tacaccgatc gttgcacaaa gggctacaac gtgaaacaag ctggaacaag gaaaatcaca
    22441 aatctgttta taatcaaaaa cataatgaac aatctgaaac attatgaaac ggcaaaaaac
    22501 actaattgat aaaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
    22561 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
    22621 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
    22681 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
    22741 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
    22801 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
    22861 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
    22921 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
    22981 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
    23041 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
    23101 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
    23161 gctggaaaat gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat
    23221 tagcggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
    23281 ggatgttcca cctcgtgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
    23341 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
    23401 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
    23461 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
    23521 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
    23581 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
    23641 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
    23701 cgaagggtaa gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt
    23761 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
    23821 cttccagaat cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg
    23881 ggcaaggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
    23941 gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat
    24001 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc
    24061 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg
    24121 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
    24181 tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
    24241 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
    24301 ctttcgccgt gcccggatga acattgttac cgacaatttt tatattgacc gatgcggcgt
    24361 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
    24421 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
    24481 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
    24541 tgatttctgc aatacctgct ttgtcatcag cacctaacaa ggttttacca tcggtggtaa
    24601 tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
    24661 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
    24721 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
    24781 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
    24841 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
    24901 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
    24961 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
    25021 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
    25081 atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat
    25141 tcaagcggtt tgccgtagaa ttaccgccct cattaggagt caccaaggtg gttaaccaca
    25201 aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa
    25261 acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg
    25321 taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct
    25381 tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt
    25441 tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg
    25501 ctatgtgaac actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa
    25561 tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat
    25621 ggaagccctg cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc
    25681 tggtggtcaa cagcaacgcg tcgccattgc tcgagcggtg gttaacaagc ctcgtctgtt
    25741 gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga
    25801 gctgaaagcg ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga
    25861 agaagcactc accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga
    25921 cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg
    25981 cgaaattaat atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc
    26041 caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa
    26101 actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca
    26161 cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc
    26221 ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga
    26281 tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga
    26341 ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg
    26401 tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt
    26461 tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac actggataac tacacgcgtc
    26521 tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca
    26581 ccctcgcctg cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca
    26641 aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc
    26701 gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct
    26761 ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg
    26821 gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac
    26881 tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta cagactttta
    26941 tccgcatcat tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc
    27001 tgccagcgat gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga
    27061 tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca
    27121 ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc
    27181 gtttgctgaa taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
    27241 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
    27301 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
    27361 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
    27421 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
    27481 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
    27541 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
    27601 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
    27661 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
    27721 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
    27781 aagctttacc ctgtcgatgg acgatgtggt agtttcttca ttcgtcaccg gaccaagtta
    27841 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
    27901 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
    27961 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
    28021 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
    28081 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
    28141 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc
    28201 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
    28261 gataaaatgc gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
    28321 agcaatctcg atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt
    28381 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
    28441 gtcaccagct gggccgatct gtggaagcca gagtacaaag gcagcctgct gttgaccgac
    28501 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
    28561 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca
    28621 gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
    28681 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg
    28741 ccgaaagaag gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
    28801 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
    28861 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa
    28921 gtggcaaacg ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
    28981 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
    29041 cgttaattca gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
    29101 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg
    29161 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac
    29221 ttttcaacaa attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    29281 ttaccaacct gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct
    29341 tcgtgtacaa acccagccgc cggatagaca tgcccggaag taccaatggc aataaagaca
    29401 tccgccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    29461 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    29521 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    29581 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    29641 gttaccagca aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg
    29701 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    29761 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    29821 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc
    29881 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    29941 aaaatacgct gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac
    30001 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    30061 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg tggaacacga
    30121 gctacaggta agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa
    30181 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    30241 agagaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    30301 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    30361 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    30421 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    30481 tggccaaact cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc
    30541 ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca
    30601 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    30661 tcaagacgtg cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg
    30721 gcatacagct taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    30781 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    30841 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    30901 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    30961 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    31021 gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    31081 gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataaa tatcgctgga
    31141 gaggaactga tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga
    31201 aacaactacg ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata
    31261 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    31321 atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac
    31381 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    31441 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    31501 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    31561 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    31621 gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg
    31681 ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg
    31741 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    31801 aaaattgcgc cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg
    31861 ttgtgggtta acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc
    31921 ggtgaaattg atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag
    31981 tgcttcctgc cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac
    32041 cgccagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    32101 caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat
    32161 gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc
    32221 cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt
    32281 tggcggctca tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc
    32341 tgcaagcgat tcaactcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    32401 ttaccggtag gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc
    32461 gctacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc
    32521 agccccaccg cttttaacat ctccagtgca cagctgttga tttcagcggg ctttttcttg
    32581 ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga
    32641 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt
    32701 ttgctcatcg gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct
    32761 aacaggtgca gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca
    32821 ccttcgccca cgctgaaact gacattgagc agcacatcgg tttgcacact gccttcctga
    32881 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    32941 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    33001 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    33061 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    33121 aagcgccgct ccgaggattg caccgataat cccggcgcta gccccttgca ccataaagac
    33181 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    33241 ctccatcacc atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag
    33301 gctcagcagt aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    33361 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    33421 aactctcagc ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg
    33481 catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    33541 ggcggcgaaa gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt
    33601 gaactggctg gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg
    33661 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    33721 cacattgacc agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac
    33781 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    33841 gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc
    33901 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    33961 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    34021 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc actgcacgcc cacgcatgta
    34081 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    34141 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    34201 agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatatt ttatcatcaa
    34261 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct
    34321 cgggtcataa ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg
    34381 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    34441 tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa
    34501 gtcctgagca aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt
    34561 tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    34621 tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag
    34681 tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc
    34741 tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc
    34801 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    34861 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    34921 cccacgccgt ggtggggaat gaacagtgtg atacgcaatt tgccttatta cagccttggc
    34981 gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    35041 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    35101 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta cgagcaacga
    35161 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    35221 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    35281 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg
    35341 ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat
    35401 ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcacc
    35461 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    35521 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    35581 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    35641 gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc
    35701 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    35761 gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    35821 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    35881 gcagcgtggc atactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag
    35941 ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt
    36001 acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga
    36061 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    36121 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    36181 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    36241 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    36301 ggaacatatt taccagcaag tgagtaaagg cacattacct gctgggatcg agtactggca
    36361 gccattgttc ttcagcgaac cactgccacc gctgttcagt tatttccctg ccaatacctt
    36421 gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc
    36481 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    36541 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgagtgc agctaaaaac
    36601 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    36661 ggccgttcag gcgcaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    36721 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    36781 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    36841 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg cgcgtaatct
    36901 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    36961 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    37021 gccggtggtc catctggagc acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc
    37081 tggcggcatt actggcgagt acctgatgct cacctatgcc aacgacgcca aactgtatgt
    37141 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    37201 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    37261 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    37321 cgcgtttaaa cacgatcgtg agcagtatca gttattctgc gacagcttcc cgtttgaaac
    37381 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    37441 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    37501 cgctttcctg gcagtagata accacaagca ggtagcggtg ttggtgccta ccacccttct
    37561 tgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    37621 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    37681 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    37741 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    37801 cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    37861 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    37921 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    37981 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    38041 cattcagaaa gccgccgaac ggctggcaga actggtgcct gaagcacgga ttgccatcgg
    38101 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    38161 tttcaacgtg ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa
    38221 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    38281 tcgcgtcgga cgttcacatc atcaggcata tgcatggctg ctgacgccgc atccaaaagc
    38341 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    38401 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    38461 agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    38521 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca
    38581 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcatcc ctgacgtgaa
    38641 cacgcgtctg tcgttctaca aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    38701 gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc gtaccctgct
    38761 ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    38821 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    38881 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    38941 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    39001 actggaagag aacgcgatcg cttaatgtaa atcgggggcg tttctgcgcc cccatctgca
    39061 acatttacaa attctttgca ctttcctgca ctatccgaca ctgtcaccat ccataattca
    39121 ggcttatctg tttattacaa taaccttata tttattatgt atttttggtg atgatcaaaa
    39181 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    39241 cggcaaaagc caacacctgg ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    39301 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    39361 tcggctttct cgctctgtta caagctaacc ccggcgttga tccttacgta ccgcgcgcgg
    39421 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    39481 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    39541 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    39601 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    39661 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    39721 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    39781 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    39841 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    39901 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    39961 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    40021 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    40081 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    40141 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    40201 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    40261 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    40321 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    40381 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    40441 cgcagcgatg aggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg
    40501 caagaaatta cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga
    40561 tattttttat aacaatcatt aattaattta aaaatcttta aatttcagtc aaatagaaag
    40621 caattatttt tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt
    40681 cattcacagt caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa
    40741 aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat
    40801 tgttctggca acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcaaccggag
    40861 ccaaagcgcc cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc
    40921 tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga
    40981 aaagcgtgga gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca
    41041 aagacacccc ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag
    41101 gagatatagc caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt
    41161 ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg
    41221 cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc
    41281 tggaaaaact gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat
    41341 aagtcgcaaa aaaggcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc
    41401 ttgttatact ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc
    41461 cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata
    41521 accgagcatt ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta
    41581 agtatcgctt tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc
    41641 ccccacaaca gtggcgacat ctttaccgcg accaccacca aactgatgcc ctatcacgcc
    41701 accagcaaca gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg
    41761 atgggtcact gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg
    41821 ggttgcagaa acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac
    41881 tgccgctacg cccaacgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt
    41941 cacgggaaca cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc
    42001 tatcagacag aagatgaaaa gcgcgggaat gactggaaat acttaaggat tcggaggagt
    42061 ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt
    42121 tgtccagggc ggataaggcg tttataccac atccgccagt gtacgtcgat taatgcaact
    42181 tcaaacgcgg acggataacg cggttaatac tccccaccag catcattaat ccggttttaa
    42241 agtaaccatg cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa
    42301 ttcgtccttc aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg
    42361 agaagttcga cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct
    42421 taccgttcat ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct
    42481 gagcacgcgg cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag
    42541 cgtaaatgtc tggatcgcga gtggtttgca gcgtcggttc caccaccagc tggttgatac
    42601 ggttagtttc aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc
    42661 ataccatcag atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag
    42721 cactggtgac catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg
    42781 ccgcagcaga gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg
    42841 ttacgttcag ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgatcg
    42901 cgttgtgcaa ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt
    42961 tcactttgcc atttgcgccc aggttggcag agtatttcag gaacagattc agcatctcct
    43021 ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacgcctg
    43081 gcgtattgaa atcgttagag gtgctaccca gcgccatgac cagggtgtca taggcgattt
    43141 tacgttccgg aaccaacagt tcacctttct cgtcgcgtag ttctgcaata gtgattgttt
    43201 tcgcttcacg atcaatatca atgacggaac ccaactggaa ctggaaacca tgattgcgcg
    43261 catgggccag atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt
    43321 gcagcagtgg tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt
    43381 tcttacgtcc cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc
    43441 cgacaatcac aatctttttc aatggcgtag tcaacgtgcc ccccttaaat ttattaacca
    43501 attgttaatt aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa
    43561 tgttcttcgc tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa
    43621 tcaagttttg ttgcttaaaa gataggcaac tttaccgggg gagatcaaaa aatagagttt
    43681 attgtttatt atttgagcaa attcggaagg gtaatatagg gtgaagcgta tatcgagggc
    43741 gtttggtcgt cctgataaga tgcgcagcat cgcatcaggc attgatgctc gactgtcgga
    43801 tgcggcgtgg acgccttatc cgacttacag aattcggagc gcatcaggta ccggcaaggt
    43861 tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc gctgtaaatg
    43921 cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg
    43981 atgcaactct tcagatgtcc gctggctgtt aagtgcttca tcattacgcg acaaaataac
    44041 cagacaacga tcgcgattct tctcgcggaa gttggtcaca cacttagtgg caatatcggc
    44101 atactcttcc gggcgatcaa tcttgccttc catgttctca taagggaaca aattagggtt
    44161 gaagatcacc tggcggatat cgcaaagaaa accaatccgt tccgcccagt atccgcccag
    44221 gccaacgccg caaattagcg gacgctcgtc aacgttcagt tgcaacattt tgtccacttc
    44281 tttaagcaga tgctgcatat catgtttcgg atgccgcgta ctgtagctta tcaagcgtac
    44341 atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc
    44401 aaaaccgtgt aaatagataa tcatcgcttc ctcacataag ccagggttag tgacctactt
    44461 tctcttcctg ccagcgttca tgtaactgat tcagacgggt gctgatcgct ttccagcgag
    44521 ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt
    44581 ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga
    44641 ccaggatcat atcgcaaccc gcatccagtg atgcctgccc gcgttcggca taactgccca
    44701 taatcgcggc accttccatc gataaatcgt cagagaaaat cacgccgtca aaccccagtt
    44761 cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc ggatcaacat
    44821 cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac
    44881 tgaagaccga catatcttta gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt
    44941 ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca
    45001 ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat
    45061 cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacatcc agcactggcg
    45121 caaagctgat atcaatatcc atagcgatca tttcgctggc catcagccaa cccgcctctt
    45181 gcgccagttt gccgccctct tccattccca acagcgcagc aaaggattgt gctgccggta
    45241 agcgggtaaa accttcgcga aaacgctgca cgcgtccacc ttcctgatct accgccacca
    45301 ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat
    45361 catgatagtt acgcgtaaag agaatcagcc ctcccaccag cggatgcgcc agtatttcac
    45421 gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc acactgctct
    45481 ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga
    45541 tcgccggttt gtcgccagcg gtactcaaac caccctcctt tgagcatcag cagccaggga
    45601 aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag
    45661 tcattgacca attgccggtg ctgttcagta ttttccaccc acaccgccgc cagttccagc
    45721 gcgatatcac catctccagc atactcccag tcgatgagtt ttaaccctga tgcgctatgc
    45781 actaaatttc cggcgtggac atccatatgc aatggactta agcgtaaagg tcgtggttcc
    45841 cgcgctttgc gcagacgttt taacattcgc agccaaccca cagtccgccg cgccggatcg
    45901 ctttgctgcc agtacagttc cagtaagggc aacagcgtta ttcgccagcc aaaacgtggt
    45961 tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt
    46021 accgcaccgg gcagatagtc gactaccatc cagtcacgga gatataaatg cggcttcggt
    46081 gcaatgcttg cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc
    46141 gcatcaggat cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa
    46201 ctaccgccac taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc
    46261 gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa acggcacctt tacctgacca
    46321 gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt
    46381 gccagaggcg caggagtaca gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc
    46441 tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct aactgttgct tagccatcga
    46501 cagttgctgg gcggaaacca gggtaaattt cccgttatta gccagcgcat ttcgcagcgt
    46561 ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag
    46621 caggacgcta cccgcagtca ccccgtcagc cccaagcatc ttactgacca tcggctgcat
    46681 tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgccgttc gatcttcgtg
    46741 ctcaattggg cctggctgct gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg
    46801 ttgtggctcg gctggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg
    46861 ttgccccaca cacccggcga gaaacatcgc cagcgcggta atcaaggcgt agcgactcat
    46921 ttttgtcatc aagattcacc cctttacaaa tatagataaa gtctgacttt gtgcgccccc
    46981 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
    47041 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
    47101 acggtaacgg gttcttgcct ttcgttataa atcgttgagg atgctgaagg ttgaatatca
    47161 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
    47221 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
    47281 aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
    47341 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
    47401 gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca ataccttctt
    47461 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
    47521 acacgtcgtt cacagtcgga atgagaatat tcggaatgat cagaatatgc gttggcgctt
    47581 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
    47641 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
    47701 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
    47761 gttaaacctg ctctttcaat cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
    47821 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
    47881 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttgca gcaaaattat
    47941 tcgcacacta aatataaccg ctcatcaccc tcctccggca tgagcctgac gacaacataa
    48001 accaagagat ttcaaatgct ttcaacacaa tttaacaggg ataatcaata tcaagtcatc
    48061 accaaaccgt cactacttgc cggttgcata gcactggcac tattaccttc tgccgctttt
    48121 gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc tccagatgat
    48181 ggcgaaaatg attacagcgt aacgtctacc tctgcgggta ccaaaatgca gatgactcaa
    48241 catgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta
    48301 caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc
    48361 gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt tgatggtatc
    48421 cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactgttt
    48481 gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga ccgggacggg taatccatct
    48541 gcggcaatta atatggttcg aaaacacgcg acctgtcgtg aatttaaagg cgatgtctcg
    48601 gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc
    48661 gaagacggta aaatccgcgc gcgaattgtc ggcggctacc agaataacga ctcatggctg
    48721 gaccgctaca atagtgaaaa gaccttcttc tcgggcattg tcgatgctga tttaggcgat
    48781 cttacgacgc tttcagccgg ttacgaatat cagcgcattg atgttaatag cccaacctgg
    48841 ggcggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg cgcacgcagt
    48901 accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag
    48961 cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca cccactctga agtcgaattt
    49021 gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg
    49081 ccatacagta attatggacc tggctttgat catgtcggcg gcaccggttg gaacagtggc
    49141 aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt gtttggtcgt
    49201 cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgttc agttcatggg
    49261 ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat ttcccacaag
    49321 ccgactggtc atcacagagc ctggcgcagg acgataccac acatatgaaa tcgttatatg
    49381 ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca cgttatacca
    49441 actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg ccttacgctg
    49501 gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc tctattttcc
    49561 agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc ggtaacaact
    49621 acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg ttagccatct
    49681 tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc ggcagcaacg
    49741 gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa aggggtggaa tttgaactca
    49801 acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat cttgcagagg
    49861 ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa atgttcacca
    49921 gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac tggcaaaatc
    49981 gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa ggtagctacg
    50041 cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta caggggaacg
    50101 tcaataacct gttcgacaaa acctacgata ccaacatgga aggttctatc gtctacggcg
    50161 caccgcgtaa tttcagcatt accggcacgt atcaattctg aataacacct gtaaaaaagg
    50221 cagccatctg gctgccttag tctccccaac gtcttacgga ttagtggtta cggatgtact
    50281 catccatctc ggttttcagg ttatcggatt tagtaccgaa aatagcctga acaccagaac
    50341 cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc actttagaca
    50401 catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta atgttttctt
    50461 taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtt gcttttgcat
    50521 cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc agcacgcgga
    50581 agatggtgta gtaaacaatc gcataaccga taccgacgat cggtaacagc cacagtttgc
    50641 tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac
    50701 gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg tggatgatgt
    50761 acaggatcgg cgcaacgaac atgaagaaga actcgatcgg ctcggtgata ccggtcagga
    50821 acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt tctggtttag
    50881 cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac aggaagccac
    50941 cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa
    51001 cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg ttccagatgt
    51061 ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta
    51121 ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca atcggcggcc
    51181 aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg atcggcacaa
    51241 agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag aaacggttaa
    51301 acatgtacgc tgcaatcgca ccggagataa tccctccgag tacgccagta tccgccaagt
    51361 gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc acggccatgg
    51421 ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca tcgttattgg
    51481 taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc
    51541 ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc gcggaaccga
    51601 cgcccagcag aatacctgcg ataggtagta cggataccgg cagcatcagc gatttaccga
    51661 ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct gagtatgggt
    51721 gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct
    51781 aagcgccctt tatttattac acagagtaaa ataattcagt gccaatttgt ttgacggcta
    51841 tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa
    51901 tatttcaaac gcggttattc accgcccaat tttcggcggt gaactttact cgttttagcc
    51961 attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga
    52021 tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac
    52081 ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc
    52141 ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaat cgatccaggg
    52201 ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca atgccggaaa
    52261 aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac
    52321 agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg
    52381 cacgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat
    52441 gatggatgaa ggactcttgc tgacgtactt tagtttccgg tgtgtaataa taatccagcc
    52501 cggtttcacc cagcgctaca acaccctctt ctgccgccag acggcgtaaa tcttctacat
    52561 cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt
    52621 cgcctaccag atcccgcata tgtaaataac ccggtaatgt tgtggcgact gccagacaaa
    52681 atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt
    52741 cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa
    52801 agatgaggaa ccggtaacac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga
    52861 tcggtgatga gaagctcgcg gttgatgcct gtgacagaca ttaactgttc acgaatgtgg
    52921 caaacatccc ccagtatagc ctgcaggcac gagggagaaa gatggtttgc cagttcggcg
    52981 accaggcccg gcacatcaac attggtcacc tgcgcagcgc catgatggcg ttttagcgca
    53041 tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc
    53101 gccagcagcg aataccagtc gcccgatggc acgctatatg ccaacgcctg acacaatgtt
    53161 tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg
    53221 cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg tgaaagccaa
    53281 gtcacggcgt actgttctgg aggcggcgca aggtaatgta accgacaacg actacgtaat
    53341 gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc tgctggtggc
    53401 tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt
    53461 acccagacaa ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc ggtgacctca
    53521 cgtaccgcat caatgcccag cgtatttttc cctttttcgg gagccagggt gtagtaatcg
    53581 ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc
    53641 tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg
    53701 cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata gctggctacc
    53761 agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttcaccc
    53821 agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa
    53881 tggtatgaat acttttatct tgtgcagcca gttccagata gcgggcgcgg gtgcgattaa
    53941 agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta
    54001 agccaacttc cggggtaaca tcgagataga gcgttaagtc tgggcgaaaa tccccgagaa
    54061 cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg ccctgatacg
    54121 cctgagtgga aagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agcgcaggtt
    54181 tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat
    54241 cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg
    54301 caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcga
    54361 gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga
    54421 cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg
    54481 ttatgactgg caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag
    54541 agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct
    54601 atcgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg
    54661 tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg
    54721 gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt
    54781 tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag ctgattttta
    54841 tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt
    54901 ttcttgtgaa ctcgcttgag taacgcgaca tcggtggtat tggcggtata catcaaggtg
    54961 tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg
    55021 gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg
    55081 aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg
    55141 ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg
    55201 gctttaaagt gagaaagatc cggttcgata cgcagcagcc attgaaaaac ccgtgggcga
    55261 ttgatgatct tatcagcata aagctgttca ccgagcgcca gacgtccggt ccctggcttc
    55321 agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc
    55381 cagacgccca caccagcggc gatacccagt accaccagca ataacaagat aatcaataac
    55441 acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg
    55501 ctgacgaaaa ggtgacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac
    55561 aaataaccat ctcatctgcc tgcaacgcct cctccagcga ggcttgcact tcgacaagct
    55621 gataagagga ttgtgccagt aaacggatac agaattgtcg cataatgccg ttaacacctg
    55681 cctgatccag tcgcggcgta taaactacat tgcccttccg ccagaacaaa ttagccgcac
    55741 agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct
    55801 caagatgaga gcgaatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat
    55861 gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg ttacgcaaac
    55921 ggtcgtaatg tgcaggataa gccgtaacgg agagaatccg cgttgctggt ccgctgttca
    55981 atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagc acaccatttt
    56041 gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag tcacaggaaa
    56101 tcatcaaccg ctgacaagca tcctgtagtc gcaggatatg cgccgataac aaactgactt
    56161 taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc
    56221 ttactgccag cgattcctgc ttataaccgt taattaagaa cataccggct ccttatcaga
    56281 gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa
    56341 aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca acttagatct ttttaaagat
    56401 caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct
    56461 gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag
    56521 gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac
    56581 tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg
    56641 gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg
    56701 cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg
    56761 cagagcattt gccattgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg
    56821 ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt
    56881 tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca gtacgaagcc
    56941 atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa
    57001 tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct ctgcgccacc
    57061 tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg
    57121 cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat
    57181 agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt
    57241 acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat
    57301 cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt caaggccaga
    57361 atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg
    57421 ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt
    57481 cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag
    57541 cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt
    57601 cacaactaca cgacgcttag acacgtttgt cctccaagga gggaaaaatg attctagtgg
    57661 gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg
    57721 tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc ttcttcgtcc
    57781 ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt gtcaagagaa
    57841 tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg cttaacgccc
    57901 agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact cttaaatttc
    57961 ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa aaatttgcaa
    58021 ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac cccccaataa
    58081 cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc aacatgagca
    58141 ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa agtttcaccc
    58201 gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg
    58261 ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc atcgctcagc
    58321 gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt aataccgcgt
    58381 gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt cgccgcagcg
    58441 tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt gataatacga
    58501 ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa aacagatgaa
    58561 aggttagttt cgataatatc gttccactct tcatctttca ttcgcattaa caggttatca
    58621 cgagtgatac cggcattatt gaccaggata tccacttcac caaattctgc gcgaattttt
    58681 tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc tttgccgttg
    58741 gcacctaaat aatcactgat cgcctgagcg ccattttcac tggtcgcagt gccaataact
    58801 ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg gcttgcaccg
    58861 gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc
    58921 gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc
    58981 gtcaggccag taagcacctt gcccgggccg acttcataga gatgttctac gccttgcgct
    59041 gccatgtact caacagactt cgtccactga accgggttat acaactgacg taccagtgcg
    59101 tcacggatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac aacagaaact
    59161 gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc tgctggtttc
    59221 atcagcgcac agtgagacgg tacgctcact ggtaacggca gcgcgcgttt tgcgccagca
    59281 gctttacagg cagcgccagc acgctcaacc gcttctttat gaccggcaat aaccacctgt
    59341 cccggagagt taaagtttac cggagaaacg acctgacctt ctgcagcttc ttcacacgct
    59401 ttcgcaatag gcgcatcatc cagaccgatg attgccgcca tagcgcccgt gccttccggt
    59461 acggcttctt gcatgaactt accgcgcatc tcaaccagac gcaccgcatc agcgaaatca
    59521 atcacgccag cgcaaaccag cgcggagtat tcccccaggc tgtgaccggc catcattgcc
    59581 ggtgctttac cgccctgctg ctgccatacg cgatacagcg caacagatgc agtcaacagc
    59641 gctggctggg tttgccaggt tttattcagt tcttcagctg acccctgctg ggtcagcgcc
    59701 cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac aattggatag
    59761 ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg gaacacaaat
    59821 gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcaga gccccaagtg
    59881 aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt
    59941 acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg gcgatccagc
    60001 gtcaccacga cattgtccat cgacataccg agttttttcg ccgttgcact gataatacgc
    60061 aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt attcgccgcc
    60121 agcgtctcat caacgatgtg cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc
    60181 gccatcgtca gatgaattga attctctgga ttcacgcggt cggcgtttgg cagcgtcagc
    60241 aattcaccat aactaccgtc ggcatgcaga tgggtggaaa tgattcccgg ctcttcagag
    60301 gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc acgatctgtt
    60361 ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt caccgcccca
    60421 gatttcacgt attgatcggc tacgcttaat gcataggtga aacctgcaca ggctgctgca
    60481 acgtcaaatg ccgggcaacc tttaatgtcc aacatgcttt gaatctgaca agctgcgctc
    60541 gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca ggccaatctg gtctttctca
    60601 atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg
    60661 tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca ctcgtcagag
    60721 gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag atagctgcca
    60781 gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat accgtcactt
    60841 gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc
    60901 cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc gctgaggaac
    60961 ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc
    61021 tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc cgttatactg
    61081 gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac gctttaataa
    61141 cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag aaaggaacat
    61201 cctgacaaca ccttccatcg tctttaacgt gacatttcct gtaaagccgt cacaaaccag
    61261 cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat aattgataga
    61321 agggattgtt ttaagcaccg ctgaggcatc ccgaatactg tcaagaccct ttacttcttc
    61381 ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct cttcagccag
    61441 aactgagccc ataatggcaa attgcaccaa cattgtgctg tcacaatcga cgttggcccc
    61501 taaatcgagg accaccgttt tgcccttttg ctgatgtggt aataccgtca ccagcgccgg
    61561 acgctcaatc ccctccaggg gcttgagtaa taattttgcc agccccatca gtgccccggt
    61621 attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca gggccatacg
    61681 cattgaactc ccacggctgg cgcggatagc ttgcgaaggc cgggcatcac tggcgataac
    61741 tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt tagcaagtaa
    61801 tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat tagaattcag
    61861 tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca tgacatctaa
    61921 cgccagggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc cccagttgcc
    61981 tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc gatgaccttg
    62041 cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc accagaagtt
    62101 ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg catgccacgt
    62161 ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca attacggttt
    62221 atccccgatc caacggggag caccaattac ttacgcttta agctggctaa tacggcaaat
    62281 gggtttggct tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact
    62341 tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat ttcatcttca
    62401 accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc atacgcttcc
    62461 ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt tgtgtagacc
    62521 tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg
    62581 ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca ttccacatca
    62641 ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt atagataccc
    62701 tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg taattttacc
    62761 ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc
    62821 tgtggttgcc ttttgccaat aattcgcaca cattgcgggt tactgcttta ttttcgttca
    62881 gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg gctatcatca
    62941 atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc acctcgccat
    63001 ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca cctgaggtcg
    63061 acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg gcacaagaaa
    63121 aatcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca gaccaggtat
    63181 gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc cgtctgcaat
    63241 tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg tttaattcgg
    63301 cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt cacctaagcg
    63361 aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta
    63421 agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac cctaacacgc
    63481 tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc
    63541 tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc ggtgcaggcg
    63601 gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct tatatgcttg
    63661 tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc catcggcgct
    63721 tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg
    63781 aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg
    63841 tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg gatctgatga
    63901 gtacgtccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg ttcttccact
    63961 ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg ttcgccgctt
    64021 tgcagaatat ttttcaataa cggcgcttga acgctcttca catgcgactg ccactgaccg
    64081 cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc atgcagagaa
    64141 cgcaacgccg agcgtttttt cgctaccagc aacacacctg aagtgtcccg gtcgagacga
    64201 tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc gataacgccg
    64261 aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag caccaggata
    64321 tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa
    64381 accgcctctt cttcccgctc agcaacgcga accggtggga tacgcacctc atcacccgct
    64441 tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcacc tttacgcaaa
    64501 atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa gttatcgata
    64561 cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt ctctgttttc
    64621 atggtgggcg attctaatta gccaacagga ttcgcgccac tcatttttct atgcttattt
    64681 ttagttttgc accttattac ttcactacgt gatcacttta ttgatggtta ttaaaccaat
    64741 caccagcaag aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa agtcatcttg
    64801 ctataacaag gcttgcagtg gaataatgag gccgtttccg tgtccatcct tgttaaaaca
    64861 agaaatttta cggaataacc cattttgccc gaccgatcat ccacgcagca atggcgtaag
    64921 acgtattgat ctttcaggca gttagcgggc tgcgggttgc agtccttacc ggtagatgga
    64981 aatatttctg gagagtaata cccagtctgt ttctttgata attgcgctgt ttttccgcat
    65041 gaaaaacggg caaccgacac tctgcgcctc tttgagctga cgataaccgt gaggttggcg
    65101 acgcgactag acacgaggcc atcggttcac acccggaaag gcgttacttt gcccgcagct
    65161 tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac
    65221 tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg acctggatat
    65281 cgaaagtcca gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat
    65341 tgaaccgagt ctggaagctg cttttgttga ttacggcgct gaacgtcacg gtttcctccc
    65401 actaaaagaa attgcccgcg aatatttccc tgctaactac agtgctcatg gtcgtcccaa
    65461 cattaaagat gtgttgcgtg aaggtcagga agtcattgtt cagatcgata aagaagagcg
    65521 cggcaacaaa ggcgcggcat taaccacctt tatcagtctg gcgggtagct atctggttct
    65581 gatgccaaat aacccgcgcg caggtggcat ttctcgccgt atcgaaggcg atgaccgtac
    65641 cgaattaaaa gaagcactgg caagccttga actgccggaa ggcatggggc ttatcgtgcg
    65701 caccgctggc gtaggcaaat ctgctgaggc gctgcaatgg gatttaagct tccgcctgaa
    65761 acactgggaa gccatcaaaa aagccgctga aagccgcccg gctccgttcc tgattcatca
    65821 ggagagcaac gtaatcgttc gcgccttccg cgattattta cgtcaggata tcggcgaaat
    65881 ccttatcgat aacccgaaag tcctcgaact ggcacgtcag catatcgctg cattaggtcg
    65941 cccggatttc agcagcaaaa tcaaactgta caccggcgag atcccgctgt tcagccacta
    66001 ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc catccggtgg
    66061 ttccattgtt atcgacagca ccgaagcgtt aacagccatc gacatcaact ccgcacgcgc
    66121 gacccgcggc ggcgatatcg aagaaactgc gtttaacacc aacctcgaag ccgccgatga
    66181 gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg attgttatcg acttcatcga
    66241 catgacgcca gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca
    66301 ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg
    66361 ccagcgcctg agcccatcac tgggtgaatc cagccatcac gtctgcccgc gctgctccgg
    66421 taccggtacc gtgcgtgaca acgaatcgct gtcgctctct attctgcgtt tgatcgaaga
    66481 agaagcgctg aaagagaaca cccaggaagt tcacgccatt gttcctgtgc caatcgcttc
    66541 ttatctgctg aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc aggacggtgt
    66601 tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg cactaccacg tgttgcgcgt
    66661 gcgcaaaggg gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc atgaagaagc
    66721 gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac aacctgcact
    66781 ggcaaccttt gccatgccgg atgtaccgcc agcgccaacc ccagctgaac ctgccgcgac
    66841 tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca gcagctcctg cacaacctgg
    66901 gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg aagaagccaa
    66961 accgaccgag caaccaacac cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg
    67021 caaacctcgt cagagcaacc gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg
    67081 tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca
    67141 aactgccgag acgcgtgaga gccgtcagca ggctgaggta acggaaaaag cacgtaccac
    67201 cgacgagcag caagtgccgc gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca
    67261 ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca
    67321 ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt
    67381 gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc
    67441 cgctgccgaa ccaattgttc aggaagctcc agctccacgc acagaactgg tgaaagtccc
    67501 gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg ctgataaccg
    67561 tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg
    67621 tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac
    67681 cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc tggatccgct atccaattgt
    67741 acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc
    67801 gatggtgact gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg cgccagttgt
    67861 tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc aaccggaagt
    67921 ggttgaaacg acgcatcctg aagtgattgc cgccgcggta actgaacagc cgcaggtgat
    67981 taccgagtct gatgttgccg tagcccagga agtcgcagaa cacgcagaac cgatggttga
    68041 accgcaggaa gagacggcag atgcagatat tgaagaagtt gccgaaactg ctgaggttgt
    68101 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    68161 agaagttgaa acggtggccg cggttgaacc agagatcacc gttgagcata accacgctac
    68221 cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga
    68281 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    68341 gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaaag
    68401 taatcaagcc ctggtaactg ccagggcttt tttatttcat gtttgaatca acatattaaa
    68461 attcatcaaa tggcatcctt gctaaccaac aatgcaaaat aggcaaaaaa aatgcccgcc
    68521 ccggcgatcc cagagcgggc agacaaaaca tacccagttt catgatatgt tttaaagcga
    68581 gcttatttgt tgagctggaa gagcgacaat ccctgcatat cggtaaatgc tttatacgat
    68641 gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag ttgcattcca gtccacatca
    68701 accagatcgc tcatctgctg cgtttgccct aaagcgcgat cgctacctaa ggaatccagc
    68761 gactccagtt tgttcagctg cgtgcctaat tccgcgcgaa cggtaaggac attattcagc
    68821 gagtttttca gtccacggtt ggttttatcc aatgccgcag cggcgatttc tttatccgct
    68881 tcgctatccg cgaccggcgt tttcagggct gcgatggcgc tatccagcat ggcaaaaaga
    68941 ttggtttcag aagcgctacc gtctggttcc gctaccgcgt tactggtaat actgtcgaaa
    69001 attttgtcgc ccgtatgtcc tatcaccatc gaacgcgaag catcgacctg ttgcttaata
    69061 ctttctgttc cgccaacata atcaccgtta gcctcactaa acggcgcagt ctctgtttta
    69121 tatgtagcgc ccattaccgt cagtggtgtt cgccagattc agcaactggt cacgaagccc
    69181 ctgaatatcc gtagccagcg aggcccggtc atcgtcactc aaggtgccat tgctggcgta
    69241 gacaattttt tcctgagcat tctggatagc agtggtgacc tggctaagta cactctcttc
    69301 cagtgacact ttttgagtgg cgaaagtacg tgccagcgtg tactggctgt tttgcgcctg
    69361 tgcctgggag agaacaacag cttgtgatgc agcaatggga tcgtctgaag ggttaacgac
    69421 tcgcttaccc gtcgacatct gctcgccgta cttcatccat tctgcctgag aattggtgat
    69481 gccgcgcatg ttttgctggt acatcatctg ggtactgaaa cgcattttat ctccccttag
    69541 cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg cgcattcgcc
    69601 aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt gacaccggaa
    69661 atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggt gctactggtt
    69721 ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc gtcgttaaag
    69781 gatttcgcac cgcccaccgt tttactgttg ctttgcagat ccagcagggc ctgaccgttg
    69841 cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg cgattttcgc ttcgtcggtg
    69901 attaatacat ccatgttgac gatggcgtca cttactggtt tcagcgtgaa gctgtcgtta
    69961 acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt accgttggca
    70021 tctggtgtcg ccgtaaaagt ggtattgctg gcaaggcggg tgacctgcca ctgattatta
    70081 tcgaacgaga ttttgtaatc tgtcgccagt accacggagg catcagttac cgtggcaccg
    70141 atcgcaacgt cacctttgtt tttcgtgttt tgcagaaccg cgggcttacc gatagtaaag
    70201 aaatcttcac cagcatcgcc gttagcatca aatccggctt tgtgttggct gttgaaagcc
    70261 tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg tctggtccag atcctgagaa
    70321 cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg gatctcaata
    70381 ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc gctggaagga
    70441 actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt ggcatcgtga
    70501 tgttataagt accgccatcc tgaacgctga cttctacgcc aacaatctgg tttaattcgc
    70561 tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc cccacgcctg
    70621 tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg ttgatctgat
    70681 caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga tattgatcgg
    70741 tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg cgcgccgccg
    70801 ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc atctgtgttg
    70861 ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc tcatagcggg
    70921 cggtcaggcc gctactttgc gtctgcgcag cacgtaactg gttggtaata aacgtatcat
    70981 actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca gcgcccaacg
    71041 tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg ttatagctgg
    71101 agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg ctcatggcgt
    71161 tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag tattatcggc
    71221 agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc tgtaggtttt
    71281 gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt tacgggcata
    71341 gtgaggatcg gtagcatagc ccgcgtcctg taggacctgc gccccctgtt ccgcactcgc
    71401 ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt aatccgacaa
    71461 tgcttccaga tacgagctgt agacccgaaa cttcgctttt actttcttcg cttcgccgtt
    71521 ttcatattca gtcgtggtga tttcagtgac ttgccctttc cagttgccag aggctttgac
    71581 accaaacagg ttatagcttg gctcgccgtt ttcgcggcgg atttgccgtt gcccccaacc
    71641 agattcaagc gcagcctgag cgaggatcaa atgatgtggc acaccgcttt gctggcttgc
    71701 cagttgggcg ggcagcgaga gttgcgcgag gaatgctcta ctgtcacccg gcagcgaatc
    71761 atcgtagtta cgtggtacgg ccttttgcac cagctgcgaa agcgtctggt tttgataacg
    71821 caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct ctggcaatgg
    71881 ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt tgcccgccgt
    71941 catctgttgg gcaatttgct ggtcatacat actggtatac agacgagtgt gctcgctgct
    72001 gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct gcacgaacat
    72061 cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc tcgctttcgc
    72121 ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt tgctgtcgct
    72181 gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat tgattgcaaa
    72241 atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcacgcac cacgttattg
    72301 aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa atcgatctgc
    72361 gtttgtggag taacaacagt ctgtccacca ccaaacggtg tatctggctg gctgacattg
    72421 gcctgacgat taactgttac tgagagattt ccctgcgcta ccgcgcagct gtcgagggtc
    72481 acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt agcgtcctgc
    72541 ggggtgacat taacctgcat attctggata tcggcaagga aacggacctg ggaactgttg
    72601 ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct gccatatcca
    72661 cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc gtcgttaagt
    72721 tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc aataaccgca
    72781 ccattggtga tccgtccacc gttcagttgg ttaacctgaa cactgctacc gcctgcggag
    72841 gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg actgtcaacg
    72901 cccttaagcg gagtcatcaa caacgtacct ccacgcaagc ttttggcatt acccatggaa
    72961 gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga cgctgtcacc
    73021 attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt aattcccagc
    73081 tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt ctggtcacca
    73141 gtgccatcca gccccactac caagccatag ccaatcagtg agttttgcct taccccctga
    73201 acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag tagaagaatt
    73261 aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg ttaaggaaga
    73321 aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct acgtattcaa
    73381 tacgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata gtgcgtggat
    73441 taaccacgcc cgagaagcga ataaattcgg taccctgatt aatggcaatc tgtttttcac
    73501 ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc aacgtgccgc
    73561 taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca ccggaggctt
    73621 cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca gtatcaaagc
    73681 caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg gcgctgacgt
    73741 tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga tcttcaaaca
    73801 gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg ttggcgacgg
    73861 gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc gtggagggta
    73921 tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa gtatgcgcag
    73981 cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct gccaccggtt
    74041 atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac cgctttactg
    74101 ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc cacgttgacg
    74161 ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc cggcgtgctt
    74221 tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat gctctccagt
    74281 ccggtatcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg agctgcctgg
    74341 ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa cgcattcgcc
    74401 ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg cccgttctga
    74461 tccacctgga aagagccgtc acggatatag gctgatgagc catctggcaa cattacctgg
    74521 aaaaagccct gccctttaat cgcgacatct ttgctgttat tggtctgcga cagattgccc
    74581 tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg taatccggag
    74641 ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg ataaagcaga
    74701 tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc caggttgttg
    74761 gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat ccataatgaa
    74821 ctgatcatat aatgtcctgt agtggattgc tgacaagcag agcagaagca cgcgacagtg
    74881 tgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaatagtc cgcgcgcttt
    74941 tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg gcacggcctg
    75001 cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc gcgctggcaa
    75061 tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc cccgacatca
    75121 cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt ggcctgggtt tctgcgctta
    75181 aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc accagtttca
    75241 gacgccctac tggcgcaact gtatttgccg gatcgcccgg attgagcgcc gagattgtgc
    75301 catcggcagc aatcgtgatt tccgcccctt ccggcacagc aattggcccc gcctcgccta
    75361 tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg ctgccattac
    75421 gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccatcctgct gcaacgcaac
    75481 gtccagcggg cgagaggtgt aatccatctt gccgggcgtc atgtctgcac ccggtgttga
    75541 cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc accggcactg cgcgcaaggc
    75601 gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc agattactgg cggttaccgc
    75661 ctgttgattc agcgtctggc tggctgctcc catcgcagta taaattgcgt gatccattga
    75721 gctatcccgt cagcgattag cgtaagttaa cccgcgtgtt gaggatctgg tcctgggttt
    75781 tgatggtctg ggcgttagac ttatagttac gctgggcaac gatcatattg accagttctt
    75841 tactgagatc gacgttggac gcttccagcg caccgttggt cagagtgcca aagtttcccg
    75901 tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag acgttgtcgc
    75961 cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc cccagcagtt
    76021 gggtttgttc gttggaattg ttgccgacaa ccgtaccgtc atcattgatt tgataactca
    76081 ccagatcgcc cggtttgtag ccattctggg tggttgccac aatgttgtta gcgccggttg
    76141 cgatattatt cgccatcgca ccatccacta atgtgccatt agcattaaat tccagtgttg
    76201 tcgctgtctt cgcaatgctg tttggatcac tgctatcctg ggtgtagacc tgccagttat
    76261 tatccccggt cttcacaaaa tagacgctca tgtcatgagc attaccctga ctgtcgaaaa
    76321 cagtcaccga accttttttg ttatagctat ccgcattgct ggcgctgaat ggcgtaacag
    76381 taggaagcgg atcactggaa ttcaggttga tctgcatcga cgccgtggtg gtagttttcg
    76441 ctgccatcag ggtattcggg atcgaaatat tggtcggatt cgccccttgc tgaatagtcg
    76501 gcggcgtacc ggttaccggg taacccgtca gctgtaaacc ttgcgtattc aacaggttac
    76561 ggttttcatc cagcttaaat tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta
    76621 ccagacggaa aaaaccgttc tggctgatag caacgtccag acctcgcccg gtgttggtgg
    76681 tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt tacccccagt cccactttcg
    76741 aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag gtggcggagt
    76801 tggcgatatt gttgccaata acatcgaggt tggtggcggc agcgtttaat ccgctaaccg
    76861 cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc tgccgtactt
    76921 cgtcgagggt ggtggtgccg taagtgccga gatccagcgt attaccgctg ttgccgcgga
    76981 tcacaccctg caccagagca aactgcagcg gctgggcaac cagttgtgta ccaccgttac
    77041 tggcgctaat cgctacattg taagaaccgt tcggcgcagt gctgccatca gtcaacgaac
    77101 cgtcccaggt gaaactgtga actccggcgg tcagttcacc aatatcaatg gtgcgcacaa
    77161 ccgcgccatt tttatcggtg atggtggccg taactttgtc tgccgcctgt tgcagctcaa
    77221 caccaaacgg cgtggtcgtg gtcacagccc cttcttcact gccggttccc gcaagaacag
    77281 ttgtgccggg gatcatcacg ccgtgaccga tcaggttact ggcctgtaac gactggctgt
    77341 tatcaatctg tccggaaata gatccgagcg tggtattgag tttttcaatc ccgctgaccg
    77401 tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg gtcgggtcct
    77461 ggtttttcag ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct gcggcgttgc
    77521 tgcccgtgag cgaactactg ctggtggtac tgacgccggt atttgtcgga tcggtggtgg
    77581 ttaccgcaat ggacatagct ttctccttta ttgaccgagc gtaagggttt tcagcatcat
    77641 gcttttcacc gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg ccgacatggt
    77701 gttaaccatc tccccgacga catcaacgtt cggcattttt atgtagccct ttgcatctgc
    77761 cagcggatta cctggttcat aaaccagttt gtccggggcc tgactttctt aacatcggca
    77821 acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt
    77881 gcccgatatg gctgtccatc aggaccggtc acgctatcag catttgccag attactggcc
    77941 gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc
    78001 agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg
    78061 ctcaacgcgc taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg
    78121 cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt
    78181 tctgcggtag gaggcgtcag cgcctgcgcc gggatgtgct gcgttgaggt catcgtcagt
    78241 gcaaccacac tggttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa
    78301 tcgatatcgc gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact
    78361 tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc
    78421 ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac
    78481 gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat
    78541 tgcggacagg tacaattcac gttgtagaaa tggctggggg cgaaaatgct gacaataaaa
    78601 cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa gtacggcgag caatctcaca
    78661 tcgcaattgc acaacttttt tagcgcccaa ctcgcggggg taagtgatga ggttcgtgtt
    78721 tctattcgta cagcgcccaa tctactaccg ccatgcgagc agccattgct ttcgatgagc
    78781 aataattccc gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga
    78841 tatttacagg ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcctattgtg
    78901 cggggaggaa agctggaagc tggcaatgtc aaactgaaac gtggacgcct ggataccctg
    78961 ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta
    79021 tcacccgatc aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga
    79081 caacgcgtca atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg
    79141 ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca tggtatcgga acaggtagtc
    79201 agcggcgttg ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac
    79261 ccgtcccttg ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc
    79321 atgagtattg atcgcacttc gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc
    79381 accgacgcgc cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac cagcaccagt
    79441 gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt
    79501 gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc
    79561 aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa ctgatcgtat
    79621 gacacgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt
    79681 aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg
    79741 gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa
    79801 gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt
    79861 gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat
    79921 tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg
    79981 ggcgaacggt cagacctcaa caccccatcg cggcagtaaa aagatttcga tctgaatatt
    80041 atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt
    80101 taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga
    80161 ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat
    80221 gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa
    80281 acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc cagtaaagca
    80341 gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg
    80401 gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca
    80461 ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca
    80521 ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaacg
    80581 cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga atgcccaacg
    80641 caaccgcact cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc atcaaacggt
    80701 tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc
    80761 caagcgccac gcccagcaca ccggagggaa actccattaa gcggtcggcg taatacatcc
    80821 aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct
    80881 ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccag
    80941 catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct
    81001 gatacaccag ctgcagaatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg
    81061 ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg
    81121 gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg
    81181 ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa
    81241 atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga
    81301 gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa acataagaga
    81361 caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa
    81421 atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag
    81481 cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc
    81541 gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta
    81601 ataaattcat cggtgttcta atccggaccc acgggcaaaa cgcctgcaag gcaggcgctg
    81661 aaagaggcga atagtctacc tagattatgg tgaattgcta ccgccagatg ttacagggtt
    81721 tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cactgcctat
    81781 tcgccggcgg tttgcggaac tgtctgattt tacacgcatt caatgaagtg ccgcgcacag
    81841 ccgacaaatc cacgttgctc aagagtgctc tgccaaccag gaatcggttt attcactaca
    81901 ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca
    81961 gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca agtggtgatc
    82021 tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc
    82081 agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc
    82141 acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac gctattgcta
    82201 cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa ctcaccgtag
    82261 agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt
    82321 tccaccagcc tttcagcatc gcacagattt tctgccagcg gtttatcgac acagacatgt
    82381 acccccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggagtgcaca
    82441 aaaaccgcat cacaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag
    82501 ctgtcacaaa ttggcagggc tttggcgcgc gtaggcgacc aggctccttg taacgtccag
    82561 tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc taatcccact
    82621 acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg
    82681 ttgtttcagt tctgccactt cgatttccag ggcttcgacg cgggtctgta aatcaccgtc
    82741 aaccatgttc gacatagccg ttaccgccgg ctgatcttca acctcaccac tgaaaagatg
    82801 catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc
    82861 ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata
    82921 cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca ataacaacgt
    82981 ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg aattacaaaa
    83041 acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg
    83101 tttgaccaga ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttc
    83161 acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc
    83221 cggcgtggtc acctgctttt ccagcaagca accaattacg cgagcttcca gggcagtaag
    83281 ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg
    83341 tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt
    83401 tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag
    83461 ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt
    83521 caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat
    83581 gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg
    83641 ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccta
    83701 gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct
    83761 tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca cgatcatgca
    83821 ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc
    83881 caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata
    83941 cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt
    84001 tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atactgtccc cgaagtgatg
    84061 ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg
    84121 aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata
    84181 tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc
    84241 aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc
    84301 aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg
    84361 ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc cacgaaccgt
    84421 ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc
    84481 gttcggcgct ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt
    84541 tgatgatgca ggacagtgcc agtgcggtca ttggcgcatt gttggggagc tggctgttgc
    84601 aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta tgtgcggcgt
    84661 tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag
    84721 gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt
    84781 actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg
    84841 gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc
    84901 tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg
    84961 ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctacaacaac
    85021 ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa
    85081 ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tttagccgtc
    85141 tgggtctggc gattggcggc gctattggtt atatcggtgg tggctggctg tttgacctgg
    85201 gcaaatcggc gcaccagcca gagcttccgt ggatgatgct gggaattatt ggcatcttca
    85261 ctttccttgc gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt ttgctggaac
    85321 gcgacgcctg atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca
    85381 aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc
    85441 gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg
    85501 cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca
    85561 gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc gacggcaaac
    85621 cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct
    85681 acgtcaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc
    85741 ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat tttgccgacc
    85801 tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttgcgt gcgctggaca
    85861 aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat
    85921 ttccgctact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc cgcgttgcta
    85981 attaccgcga taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt
    86041 aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg
    86101 ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc
    86161 atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg
    86221 ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg
    86281 cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat
    86341 attgttctga caaacttgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta
    86401 accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc gaccatgaaa
    86461 ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag
    86521 atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg
    86581 cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg
    86641 cgcgaagatg gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg
    86701 aagccaggcg aaattgtcat tccgtttacg gattaatgac gagggcgcat tcgcgccctt
    86761 tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct
    86821 tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca
    86881 gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc
    86941 agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt
    87001 gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg gcacgatttc
    87061 accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga
    87121 tttaatgaag ccgtgttcac cgctgcaaaa ctttacccgg caaacgcaac cactaactcc
    87181 agccacggcg tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc
    87241 ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt
    87301 gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa
    87361 gtcgtttttg agcacatcac caccaaagat gctgccgact atgtccgtga cggaaatgaa
    87421 cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt
    87481 ggtggcgtgc gtccgcacct gtattgttta cccatcctca aacgcaatat tcaccaacag
    87541 gcattgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg
    87601 ccacatgcac gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt caacgcccca
    87661 accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa
    87721 gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc
    87781 gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga tgacaccctg
    87841 gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa atcgtagctt
    87901 cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat
    87961 tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc
    88021 cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt
    88081 atcggtacgt tatgccgcag cgaataattt atcggttatt ggcgcaacaa aagaagataa
    88141 acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag
    88201 cgaataatat gcagtgattt ttgtttttgc cggatcgccc cggctttttt attttactta
    88261 cgaattttga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa
    88321 atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa tttatctgtt
    88381 ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat
    88441 aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg
    88501 aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg
    88561 atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg
    88621 aagggactga agttggtcag acgctctggt taaccactga tgttgccaga caatttattt
    88681 cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc
    88741 ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat
    88801 ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga
    88861 aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt
    88921 attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc
    88981 aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa
    89041 agtatgtccc gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc cgccacaacg
    89101 aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct gactccgcat
    89161 ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg
    89221 cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat
    89281 ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg
    89341 cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt cgtcatgacg
    89401 atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa gcgattgtct
    89461 gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag
    89521 tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta
    89581 ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga
    89641 agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg
    89701 cggaagtggc cagtgatggg tcagaagcct tcccgttctt gcgcaatgta ttgccgggta
    89761 tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta
    89821 ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg
    89881 gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gaccaaaaaa
    89941 gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc
    90001 cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa
    90061 taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg caattatgga
    90121 tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat
    90181 ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc
    90241 agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa
    90301 ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc
    90361 gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc attcacaaat
    90421 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
    90481 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
    90541 cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt
    90601 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
    90661 actcgtcttg ccgcaaaagc tggacatctc gcattgtgcc tattgctgtt cgccatcggc
    90721 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
    90781 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
    90841 ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat ggccctgaag
    90901 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
    90961 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
    91021 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
    91081 gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt taaagatttc
    91141 gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt
    91201 aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg cagtgcggat
    91261 tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
    91321 ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg
    91381 ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg tgcaggcttt
    91441 gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt aggtccagct
    91501 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat
    91561 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatacccagt
    91621 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
    91681 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa
    91741 caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg gcagccatca
    91801 tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc
    91861 tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
    91921 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc
    91981 cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
    92041 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
    92101 ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact
    92161 tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc aagcatggcg
    92221 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
    92281 ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg
    92341 tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc
    92401 tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
    92461 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
    92521 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
    92581 tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact
    92641 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
    92701 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
    92761 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
    92821 atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata tgacgaatct
    92881 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
    92941 cgtactttgg ttggtcgtgc aattgcccta cccgattatc taccgcctcg gttgtggatt
    93001 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
    93061 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
    93121 atccgttggc atgggcctga tggaaaccgg tatggcgtgg ttctggccgg accgccgaat
    93181 cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
    93241 cggcatcctg ttagttggca tccattttct gacactggag ctgggcgcgc ggcagtttgg
    93301 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
    93361 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
    93421 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc atgattacgg
    93481 cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg
    93541 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
    93601 gccagatggc aaagggtatc aattgattat gctgccacca gagtgttctc cgccactgga
    93661 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
    93721 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
    93781 acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta ccacagcaaa
    93841 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
    93901 ttagtgtgtt tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
    93961 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
    94021 gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct tacccctcta
    94081 cgttgagcag cttggtgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt
    94141 cagtataatg ggttcctaaa cacatcattt taggtattga atatgagacg ggcaatcagc
    94201 tacatcagat tttcaagtga acgacaactg aaaggggact ctgtacgccg acaatctaag
    94261 ctggttactg actggcttga taagaaccct gagttttact tggattcatc cttgagcttt
    94321 aaagatttgg ggaaaagtgc ttttagtggt aagcacctga aaggtggtct aggtgacttt
    94381 ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctactaat cgagagttta
    94441 gaccgtctgt cacgtcagga tattgatatt gcttctgagc tattacgccg tatcttaagg
    94501 gctggtgtag atgtagtcac actgagcgat ggagaacatt acacacgaga aagtttaaaa
    94561 gacccgttag ccctgattaa aagcattctg ataatgcagc gggcgcatga ggaaagtttg
    94621 agaaagtctg agcgtgtcca agctgcatgg aaccgtaaaa aagaactcat ctccgaaggt
    94681 atcaaggtat ctaggcgttg tcctgcatgg ttaaggctga atgatgaccg tcgaacattc
    94741 acaatcattc ctgataaagt ggaagttgtt aaacgagcat ttgacctgag attacagggt
    94801 ttaagctttt gggctatcac cagaacttta aacgatgaag gtcacttgtc attgaatcaa
    94861 tacacaccca aacaaaaggg gtggtctgat actgctgtaa agaaactgct cagaaatcgt
    94921 gctgtgattg gatgtttcac acctgctgga cgtgaagaag ttcagggtta ctaccccgca
    94981 ataatcagtg agtcactatt ctatcgagta cagcaactga acacagggca atatggtagg
    95041 gcttctgtat caagtaatcc gctatcagtc aacttgttca ggggtatcat caa
//
LOCUS       DAZSNG010000002        82042 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZSNG010000002 DAZSNG010000000
VERSION     DAZSNG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.002"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHW4Z_000107"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHW4Z_000107"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR8879925.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHW4Z_000108"
     CDS             complement(1672..2667)
                     /locus_tag="ACHW4Z_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8879926.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHW4Z_000109"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHW4Z_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHW4Z_000110"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHW4Z_000110"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR8879927.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHW4Z_000111"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHW4Z_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR8879928.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHW4Z_000112"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHW4Z_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR8879929.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHW4Z_000113"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHW4Z_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR8879930.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHW4Z_000114"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHW4Z_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR8879931.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHW4Z_000115"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHW4Z_000115"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR8879932.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHW4Z_000116"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHW4Z_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR8879933.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHW4Z_000117"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHW4Z_000117"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR8879934.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHW4Z_000118"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHW4Z_000118"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR8879935.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHW4Z_000119"
     CDS             complement(17699..18322)
                     /locus_tag="ACHW4Z_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR8879936.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHW4Z_000120"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHW4Z_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR8879937.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHW4Z_000121"
     CDS             complement(18971..19213)
                     /locus_tag="ACHW4Z_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR8879938.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHW4Z_000122"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHW4Z_000122"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR8879939.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="ACHW4Z_000123"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="ACHW4Z_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR8879940.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACHW4Z_000124"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACHW4Z_000124"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR8879941.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="ACHW4Z_000125"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="ACHW4Z_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="ACHW4Z_000126"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="ACHW4Z_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR8879942.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="ACHW4Z_000127"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="ACHW4Z_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="ACHW4Z_000128"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="ACHW4Z_000128"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8879943.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="ACHW4Z_000129"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="ACHW4Z_000129"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8879944.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..29673)
                     /locus_tag="ACHW4Z_000130"
     CDS             complement(29257..29673)
                     /locus_tag="ACHW4Z_000130"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR8879945.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(29670..29777)
                     /locus_tag="ACHW4Z_000131"
     CDS             complement(29670..29777)
                     /locus_tag="ACHW4Z_000131"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879946.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(29738..30340)
                     /gene="ypdF"
                     /locus_tag="ACHW4Z_000132"
                     /pseudo
     CDS             complement(29738..30340)
                     /gene="ypdF"
                     /locus_tag="ACHW4Z_000132"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="ACHW4Z_000133"
     CDS             complement(30355..31602)
                     /locus_tag="ACHW4Z_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR8879947.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="ACHW4Z_000134"
     CDS             complement(31624..31950)
                     /locus_tag="ACHW4Z_000134"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR8879948.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="ACHW4Z_000135"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="ACHW4Z_000135"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR8879949.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="ACHW4Z_000136"
     CDS             33338..34594
                     /locus_tag="ACHW4Z_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR8879950.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /gene="ypeC"
                     /locus_tag="ACHW4Z_000137"
     CDS             34709..35035
                     /gene="ypeC"
                     /locus_tag="ACHW4Z_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR8879951.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="ACHW4Z_000138"
     CDS             complement(35176..36414)
                     /locus_tag="ACHW4Z_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR8879952.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="ACHW4Z_000139"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="ACHW4Z_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR8879953.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="ACHW4Z_000140"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="ACHW4Z_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(40322..40397)
                     /locus_tag="ACHW4Z_000141"
     tRNA            complement(40322..40397)
                     /locus_tag="ACHW4Z_000141"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="ACHW4Z_000142"
     tRNA            complement(40437..40512)
                     /locus_tag="ACHW4Z_000142"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="ACHW4Z_000143"
     CDS             40733..41092
                     /locus_tag="ACHW4Z_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8879954.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="ACHW4Z_000144"
     CDS             41094..41486
                     /locus_tag="ACHW4Z_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8879955.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="ACHW4Z_000145"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="ACHW4Z_000145"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR8879956.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="ACHW4Z_000146"
     tRNA            43212..43287
                     /locus_tag="ACHW4Z_000146"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="ACHW4Z_000147"
     tRNA            43332..43407
                     /locus_tag="ACHW4Z_000147"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="ACHW4Z_000148"
     tRNA            43412..43487
                     /locus_tag="ACHW4Z_000148"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Lys,seq:ttt)
     gene            43518..43643
                     /locus_tag="ACHW4Z_000149"
     ncRNA           43518..43643
                     /ncRNA_class="other"
                     /locus_tag="ACHW4Z_000149"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43763..44104
                     /locus_tag="ACHW4Z_000150"
     CDS             43763..44104
                     /locus_tag="ACHW4Z_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR8879957.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44095..45021)
                     /locus_tag="ACHW4Z_000151"
     CDS             complement(44095..45021)
                     /locus_tag="ACHW4Z_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8879958.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45111..46109
                     /locus_tag="ACHW4Z_000152"
     CDS             45111..46109
                     /locus_tag="ACHW4Z_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR8879959.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46106..46324)
                     /locus_tag="ACHW4Z_000153"
     CDS             complement(46106..46324)
                     /locus_tag="ACHW4Z_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR8879960.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="ACHW4Z_000154"
     CDS             complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="ACHW4Z_000154"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR8879961.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="ACHW4Z_000155"
     CDS             complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="ACHW4Z_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR8879962.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49628..50389
                     /gene="cysZ"
                     /locus_tag="ACHW4Z_000156"
     CDS             49628..50389
                     /gene="cysZ"
                     /locus_tag="ACHW4Z_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR8879963.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWFDCYRDKHAMWR"
     gene            50574..51545
                     /gene="cysK"
                     /locus_tag="ACHW4Z_000157"
     CDS             50574..51545
                     /gene="cysK"
                     /locus_tag="ACHW4Z_000157"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR8879964.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51929..52186
                     /gene="ptsH"
                     /locus_tag="ACHW4Z_000158"
     CDS             51929..52186
                     /gene="ptsH"
                     /locus_tag="ACHW4Z_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR8879965.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52231..53958
                     /gene="ptsI"
                     /locus_tag="ACHW4Z_000159"
     CDS             52231..53958
                     /gene="ptsI"
                     /locus_tag="ACHW4Z_000159"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR8879966.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53999..54508
                     /gene="crr"
                     /locus_tag="ACHW4Z_000160"
     CDS             53999..54508
                     /gene="crr"
                     /locus_tag="ACHW4Z_000160"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR8879967.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="ACHW4Z_000161"
                     /gene_synonym="thiJ"
     CDS             complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="ACHW4Z_000161"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR8879968.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55506..55880
                     /locus_tag="ACHW4Z_000162"
     CDS             55506..55880
                     /locus_tag="ACHW4Z_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR8879969.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55913..56636
                     /locus_tag="ACHW4Z_000163"
                     /pseudo
     CDS             55913..56636
                     /locus_tag="ACHW4Z_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="ACHW4Z_000164"
     CDS             complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="ACHW4Z_000164"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR8879970.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="ACHW4Z_000165"
     CDS             complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="ACHW4Z_000165"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR8879971.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="ACHW4Z_000166"
     CDS             complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="ACHW4Z_000166"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR8879972.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="ACHW4Z_000167"
     CDS             complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="ACHW4Z_000167"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR8879973.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="ACHW4Z_000168"
     CDS             complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="ACHW4Z_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR8879974.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="ACHW4Z_000169"
     CDS             complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="ACHW4Z_000169"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR8879975.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62920..63778)
                     /gene="murR"
                     /locus_tag="ACHW4Z_000170"
                     /pseudo
     CDS             complement(62920..63778)
                     /gene="murR"
                     /locus_tag="ACHW4Z_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            63942..64838
                     /gene="murQ"
                     /locus_tag="ACHW4Z_000171"
     CDS             63942..64838
                     /gene="murQ"
                     /locus_tag="ACHW4Z_000171"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR8879976.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64842..66266
                     /gene="murP"
                     /locus_tag="ACHW4Z_000172"
     CDS             64842..66266
                     /gene="murP"
                     /locus_tag="ACHW4Z_000172"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR8879977.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            66271..66357
                     /locus_tag="ACHW4Z_000173"
                     /pseudo
     CDS             66271..66357
                     /locus_tag="ACHW4Z_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="ACHW4Z_000174"
     CDS             complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="ACHW4Z_000174"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR8879978.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67441..68016)
                     /locus_tag="ACHW4Z_000175"
     CDS             complement(67441..68016)
                     /locus_tag="ACHW4Z_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR8879979.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68077..68526)
                     /locus_tag="ACHW4Z_000176"
     CDS             complement(68077..68526)
                     /locus_tag="ACHW4Z_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR8879980.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68513..68938)
                     /locus_tag="ACHW4Z_000177"
     CDS             complement(68513..68938)
                     /locus_tag="ACHW4Z_000177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR8879981.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69152..70021
                     /gene="amiA"
                     /locus_tag="ACHW4Z_000178"
     CDS             69152..70021
                     /gene="amiA"
                     /locus_tag="ACHW4Z_000178"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR8879982.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70025..70924
                     /gene="hemF"
                     /locus_tag="ACHW4Z_000179"
     CDS             70025..70924
                     /gene="hemF"
                     /locus_tag="ACHW4Z_000179"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR8879983.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="ACHW4Z_000180"
                     /pseudo
     CDS             complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="ACHW4Z_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="ACHW4Z_000181"
     CDS             complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="ACHW4Z_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR8879984.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="ACHW4Z_000182"
     CDS             complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="ACHW4Z_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR8879985.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73210..73278)
                     /locus_tag="ACHW4Z_000183"
                     /pseudo
     CDS             complement(73210..73278)
                     /locus_tag="ACHW4Z_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74382..75173
                     /locus_tag="ACHW4Z_000184"
     CDS             74382..75173
                     /locus_tag="ACHW4Z_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879986.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75893..76288
                     /locus_tag="ACHW4Z_000185"
     CDS             75893..76288
                     /locus_tag="ACHW4Z_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR8879987.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76306..76569
                     /locus_tag="ACHW4Z_000186"
     CDS             76306..76569
                     /locus_tag="ACHW4Z_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879988.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76592..77134
                     /locus_tag="ACHW4Z_000187"
     CDS             76592..77134
                     /locus_tag="ACHW4Z_000187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879989.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77273..77560
                     /locus_tag="ACHW4Z_000188"
     CDS             77273..77560
                     /locus_tag="ACHW4Z_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879990.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77599..77805
                     /locus_tag="ACHW4Z_000189"
     CDS             77599..77805
                     /locus_tag="ACHW4Z_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8879991.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78117..78747
                     /locus_tag="ACHW4Z_000190"
                     /pseudo
     CDS             78117..78747
                     /locus_tag="ACHW4Z_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            78854..80314
                     /gene="gadC"
                     /locus_tag="ACHW4Z_000191"
     CDS             78854..80314
                     /gene="gadC"
                     /locus_tag="ACHW4Z_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR8879992.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80348..81157
                     /gene="murI"
                     /locus_tag="ACHW4Z_000192"
     CDS             80348..81157
                     /gene="murI"
                     /locus_tag="ACHW4Z_000192"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8879993.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81276..81970)
                     /locus_tag="ACHW4Z_000193"
     CDS             complement(join(81276..81724,81724..81970))
                     /locus_tag="ACHW4Z_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8879994.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
BASE COUNT        21362 a        20710 c        19441 g        20529 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctt aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    43501 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
    43561 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
    43621 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
    43681 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
    43741 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
    43801 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
    43861 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
    43921 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
    43981 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
    44041 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
    44101 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
    44161 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    44221 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
    44281 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    44341 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
    44401 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    44461 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    44521 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    44581 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    44641 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    44701 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    44761 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    44821 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    44881 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
    44941 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    45001 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
    45061 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    45121 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
    45181 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
    45241 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    45301 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
    45361 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    45421 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    45481 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    45541 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    45601 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
    45661 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    45721 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    45781 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    45841 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    45901 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    45961 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    46021 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    46081 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    46141 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    46201 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    46261 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    46321 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    46381 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    46441 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    46501 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    46561 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    46621 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    46681 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    46741 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    46801 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    46861 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    46921 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    46981 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    47041 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    47101 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    47161 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    47221 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    47281 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    47341 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    47401 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    47461 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    47521 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    47581 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    47641 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    47701 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    47761 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    47821 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    47881 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    47941 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    48001 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    48061 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    48121 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    48181 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    48241 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    48301 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    48361 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    48421 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    48481 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    48541 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    48601 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    48661 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    48721 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    48781 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    48841 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    48901 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    48961 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    49021 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    49081 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    49141 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    49201 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    49261 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    49321 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    49381 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    49441 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    49501 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    49561 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    49621 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    49681 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    49741 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    49801 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    49861 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    49921 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    49981 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    50041 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    50101 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    50161 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    50221 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    50281 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    50341 ggcgatgtgg ttcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    50401 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    50461 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    50521 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    50581 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    50641 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    50701 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    50761 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    50821 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    50881 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    50941 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    51001 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    51061 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    51121 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    51181 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    51241 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    51301 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    51361 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    51421 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    51481 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    51541 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    51601 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    51661 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    51721 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    51781 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    51841 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    51901 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    51961 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    52021 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    52081 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    52141 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    52201 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    52261 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    52321 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    52381 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    52441 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    52501 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    52561 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    52621 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    52681 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    52741 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    52801 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    52861 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    52921 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    52981 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    53041 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    53101 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    53161 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    53221 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    53281 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    53341 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    53401 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    53461 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    53521 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    53581 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    53641 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    53701 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    53761 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    53821 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    53881 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    53941 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    54001 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    54061 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    54121 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    54181 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    54241 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    54301 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    54361 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    54421 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    54481 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    54541 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    54601 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    54661 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    54721 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    54781 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    54841 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    54901 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    54961 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    55021 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    55081 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    55141 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    55201 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    55261 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    55321 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    55381 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    55441 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    55501 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    55561 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    55621 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    55681 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    55741 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    55801 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    55861 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    55921 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    55981 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    56041 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    56101 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    56161 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    56221 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    56281 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    56341 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    56401 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    56461 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    56521 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    56581 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    56641 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    56701 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    56761 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    56821 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    56881 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    56941 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    57001 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    57061 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    57121 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    57181 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    57241 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    57301 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    57361 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    57421 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    57481 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    57541 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    57601 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    57661 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    57721 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    57781 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    57841 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    57901 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    57961 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    58021 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    58081 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    58141 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    58201 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    58261 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    58321 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    58381 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    58441 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    58501 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    58561 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    58621 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    58681 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    58741 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    58801 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    58861 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    58921 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    58981 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    59041 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    59101 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    59161 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    59221 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    59281 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    59341 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    59401 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    59461 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    59521 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    59581 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    59641 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    59701 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    59761 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    59821 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    59881 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    59941 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    60001 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    60061 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    60121 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    60181 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    60241 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    60301 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    60361 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    60421 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    60481 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    60541 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    60601 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    60661 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    60721 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    60781 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    60841 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    60901 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    60961 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    61021 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    61081 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    61141 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    61201 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    61261 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    61321 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    61381 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    61441 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    61501 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    61561 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    61621 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    61681 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    61741 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    61801 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    61861 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    61921 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    61981 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    62041 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    62101 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    62161 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    62221 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    62281 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    62341 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    62401 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    62461 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    62521 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    62581 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    62641 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    62701 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    62761 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    62821 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    62881 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    62941 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    63001 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    63061 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    63121 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    63181 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    63241 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    63301 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    63361 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    63421 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    63481 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    63541 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    63601 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    63661 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    63721 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    63781 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    63841 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    63901 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    63961 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    64021 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    64081 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    64141 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    64201 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    64261 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    64321 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    64381 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    64441 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    64501 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    64561 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    64621 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    64681 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    64741 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    64801 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    64861 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    64921 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    64981 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    65041 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    65101 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    65161 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    65221 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    65281 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    65341 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    65401 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    65461 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    65521 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    65581 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    65641 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    65701 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    65761 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    65821 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    65881 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    65941 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    66001 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    66061 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    66121 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    66181 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    66241 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    66301 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    66361 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    66421 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    66481 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    66541 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    66601 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    66661 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    66721 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    66781 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    66841 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    66901 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    66961 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    67021 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    67081 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    67141 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    67201 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    67261 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    67321 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    67381 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    67441 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    67501 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    67561 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    67621 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    67681 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    67741 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    67801 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    67861 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    67921 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    67981 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    68041 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    68101 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    68161 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    68221 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    68281 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    68341 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    68401 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    68461 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    68521 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    68581 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    68641 agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    68701 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    68761 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    68821 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    68881 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    68941 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    69001 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    69061 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    69121 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    69181 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    69241 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    69301 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    69361 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    69421 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    69481 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    69541 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    69601 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    69661 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    69721 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    69781 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    69841 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    69901 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    69961 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    70021 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    70081 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    70141 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    70201 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    70261 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    70321 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    70381 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    70441 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    70501 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    70561 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    70621 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    70681 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    70741 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    70801 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    70861 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    70921 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    70981 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    71041 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    71101 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    71161 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    71221 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    71281 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    71341 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    71401 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    71461 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    71521 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    71581 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    71641 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    71701 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    71761 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    71821 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    71881 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    71941 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    72001 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    72061 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    72121 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    72181 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    72241 ttgcttcggc tggcggttaa agccagtaac cagccactgg gtgtcatcgt ctggccgacc
    72301 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    72361 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    72421 cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg ctgcatctat
    72481 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    72541 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    72601 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    72661 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    72721 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    72781 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    72841 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    72901 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    72961 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    73021 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    73081 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    73141 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    73201 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    73261 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    73321 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    73381 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    73441 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    73501 actagccgca aaaatgcggt cctatttgcc agaacctttt attattcaat tggttagtgc
    73561 aagaaaaagc gtgattactg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    73621 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    73681 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    73741 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    73801 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    73861 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    73921 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    73981 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    74041 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    74101 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    74161 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    74221 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    74281 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    74341 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    74401 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    74461 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg aaaagagata
    74521 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    74581 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    74641 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    74701 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    74761 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    74821 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    74881 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    74941 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    75001 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    75061 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    75121 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    75181 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    75241 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    75301 ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa
    75361 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    75421 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    75481 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    75541 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    75601 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    75661 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    75721 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    75781 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    75841 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    75901 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    75961 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    76021 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    76081 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    76141 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    76201 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    76261 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    76321 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    76381 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    76441 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    76501 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    76561 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    76621 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    76681 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    76741 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    76801 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    76861 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    76921 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    76981 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    77041 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    77101 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    77161 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    77221 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    77281 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    77341 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    77401 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    77461 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    77521 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    77581 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    77641 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    77701 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    77761 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    77821 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    77881 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    77941 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    78001 tgatatgttt atggttttat attaattaaa atatatctaa atttgatagt tttaaaattg
    78061 caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga
    78121 aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc
    78181 tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat
    78241 tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt
    78301 tctgtcaaac atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa
    78361 acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta
    78421 tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg
    78481 gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac
    78541 gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat
    78601 aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg
    78661 ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg tgtcgttacg
    78721 actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt
    78781 aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt taaaaatgtt
    78841 cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg
    78901 gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc
    78961 agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc
    79021 tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg
    79081 atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct
    79141 acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat
    79201 tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt
    79261 cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc
    79321 gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat
    79381 tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg
    79441 tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct
    79501 tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa
    79561 aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag
    79621 tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt
    79681 ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc
    79741 atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac
    79801 aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg
    79861 tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt
    79921 tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt
    79981 tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc
    80041 tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg
    80101 attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac
    80161 tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat ttttattaat
    80221 tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga
    80281 accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag
    80341 gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg tcggtggatt
    80401 gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga
    80461 taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa
    80521 aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat
    80581 atcctcaact ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat
    80641 cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg
    80701 tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt
    80761 gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga
    80821 aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat
    80881 ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa
    80941 tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat
    81001 ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt
    81061 gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat
    81121 tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtac accgtaaaat
    81181 cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg
    81241 gacaaaccca gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata
    81301 aacgctccga tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga
    81361 cccaaccgct taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
    81421 attttgccgg tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg
    81481 ctggtcagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
    81541 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    81601 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    81661 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    81721 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    81781 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    81841 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    81901 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    81961 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    82021 catcacccat ccagcattat aa
//
LOCUS       DAZSNG010000003        75634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZSNG010000003 DAZSNG010000000
VERSION     DAZSNG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75634
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.003"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(435..641)
                     /locus_tag="ACHW4Z_000194"
     CDS             complement(435..641)
                     /locus_tag="ACHW4Z_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR8879995.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACHW4Z_000195"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACHW4Z_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR8879996.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHW4Z_000196"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHW4Z_000196"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR8879997.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHW4Z_000197"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHW4Z_000197"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHW4Z_000198"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHW4Z_000198"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR8879998.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHW4Z_000199"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHW4Z_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHW4Z_000200"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHW4Z_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8884..9018
                     /locus_tag="ACHW4Z_000201"
     CDS             8884..9018
                     /locus_tag="ACHW4Z_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR8879999.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            8997..9101
                     /gene="speFL"
                     /locus_tag="ACHW4Z_000202"
     CDS             8997..9101
                     /gene="speFL"
                     /locus_tag="ACHW4Z_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR8880000.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="ACHW4Z_000203"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="ACHW4Z_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="ACHW4Z_000204"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="ACHW4Z_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR8880001.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="ACHW4Z_000205"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="ACHW4Z_000205"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR8880002.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="ACHW4Z_000206"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="ACHW4Z_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR8880003.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="ACHW4Z_000207"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="ACHW4Z_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8880004.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="ACHW4Z_000208"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="ACHW4Z_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR8880005.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="ACHW4Z_000209"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="ACHW4Z_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR8880006.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="ACHW4Z_000210"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="ACHW4Z_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR8880007.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="ACHW4Z_000211"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="ACHW4Z_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR8880008.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /gene="chiQ"
                     /locus_tag="ACHW4Z_000212"
     CDS             complement(18063..18389)
                     /gene="chiQ"
                     /locus_tag="ACHW4Z_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR8880009.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="ACHW4Z_000213"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="ACHW4Z_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR8880010.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="ACHW4Z_000214"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="ACHW4Z_000214"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR8880011.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="ACHW4Z_000215"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="ACHW4Z_000215"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR8880012.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="ACHW4Z_000216"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="ACHW4Z_000216"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR8880013.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="ACHW4Z_000217"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="ACHW4Z_000217"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8880014.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="ACHW4Z_000218"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="ACHW4Z_000218"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR8880015.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="ACHW4Z_000219"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="ACHW4Z_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(28645..28743)
                     /locus_tag="ACHW4Z_000220"
                     /pseudo
     CDS             complement(28645..28743)
                     /locus_tag="ACHW4Z_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="ACHW4Z_000221"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="ACHW4Z_000221"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR8880016.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="ACHW4Z_000222"
     tRNA            31034..31110
                     /locus_tag="ACHW4Z_000222"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="ACHW4Z_000223"
     tRNA            31119..31203
                     /locus_tag="ACHW4Z_000223"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="ACHW4Z_000224"
     tRNA            31227..31301
                     /locus_tag="ACHW4Z_000224"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="ACHW4Z_000225"
     tRNA            31336..31410
                     /locus_tag="ACHW4Z_000225"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="ACHW4Z_000226"
     tRNA            31426..31502
                     /locus_tag="ACHW4Z_000226"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="ACHW4Z_000227"
     tRNA            31551..31625
                     /locus_tag="ACHW4Z_000227"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="ACHW4Z_000228"
     tRNA            31663..31737
                     /locus_tag="ACHW4Z_000228"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="ACHW4Z_000229"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="ACHW4Z_000229"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR8880017.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="ACHW4Z_000230"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="ACHW4Z_000230"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR8880018.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34789..35829
                     /locus_tag="ACHW4Z_000231"
     CDS             34789..35829
                     /locus_tag="ACHW4Z_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR8880019.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="ACHW4Z_000232"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="ACHW4Z_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR8880020.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="ACHW4Z_000233"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="ACHW4Z_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR8880021.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="ACHW4Z_000234"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="ACHW4Z_000234"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR8880022.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="ACHW4Z_000235"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="ACHW4Z_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR8880023.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="ACHW4Z_000236"
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="ACHW4Z_000236"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR8880024.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="ACHW4Z_000237"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="ACHW4Z_000237"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR8880025.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="ACHW4Z_000238"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="ACHW4Z_000238"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR8880026.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="ACHW4Z_000239"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="ACHW4Z_000239"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR8880027.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /gene="hscC"
                     /locus_tag="ACHW4Z_000240"
                     /pseudo
     CDS             43575..45245
                     /gene="hscC"
                     /locus_tag="ACHW4Z_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="ACHW4Z_000241"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="ACHW4Z_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR8880028.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="ACHW4Z_000242"
     CDS             complement(46753..47460)
                     /locus_tag="ACHW4Z_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8880029.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="ACHW4Z_000243"
     CDS             47562..48116
                     /locus_tag="ACHW4Z_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8880030.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="ACHW4Z_000244"
     CDS             complement(48126..49553)
                     /locus_tag="ACHW4Z_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR8880031.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="ACHW4Z_000245"
     CDS             complement(49550..50257)
                     /locus_tag="ACHW4Z_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8880032.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /gene="ybeQ"
                     /locus_tag="ACHW4Z_000246"
     CDS             50421..51398
                     /gene="ybeQ"
                     /locus_tag="ACHW4Z_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR8880033.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="ACHW4Z_000247"
     CDS             complement(51468..51950)
                     /locus_tag="ACHW4Z_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR8880034.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="ACHW4Z_000248"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="ACHW4Z_000248"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR8880035.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="ACHW4Z_000249"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="ACHW4Z_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR8880036.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="ACHW4Z_000250"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="ACHW4Z_000250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR8880037.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="ACHW4Z_000251"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="ACHW4Z_000251"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR8880038.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="ACHW4Z_000252"
     CDS             57061..57672
                     /locus_tag="ACHW4Z_000252"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR8880039.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="ACHW4Z_000253"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="ACHW4Z_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR8880040.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="ACHW4Z_000254"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="ACHW4Z_000254"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR8880041.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="ACHW4Z_000255"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="ACHW4Z_000255"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR8880042.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="ACHW4Z_000256"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="ACHW4Z_000256"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR8880043.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="ACHW4Z_000257"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="ACHW4Z_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR8880044.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="ACHW4Z_000258"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="ACHW4Z_000258"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR8880045.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /gene="ybeD"
                     /locus_tag="ACHW4Z_000259"
     CDS             64327..64590
                     /gene="ybeD"
                     /locus_tag="ACHW4Z_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR8880046.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="ACHW4Z_000260"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="ACHW4Z_000260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR8880047.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="ACHW4Z_000261"
     CDS             65591..66544
                     /locus_tag="ACHW4Z_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR8880048.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="ACHW4Z_000262"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="ACHW4Z_000262"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR8880049.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="ACHW4Z_000263"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="ACHW4Z_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR8880050.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="ACHW4Z_000264"
     CDS             complement(68151..68939)
                     /locus_tag="ACHW4Z_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR8880051.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="ACHW4Z_000265"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="ACHW4Z_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR8880052.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="ACHW4Z_000266"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="ACHW4Z_000266"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8880053.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="ACHW4Z_000267"
     CDS             complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="ACHW4Z_000267"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR8880054.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="ACHW4Z_000268"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="ACHW4Z_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8880055.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="ACHW4Z_000269"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="ACHW4Z_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="ACHW4Z_000270"
     CDS             complement(73107..73616)
                     /locus_tag="ACHW4Z_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8880056.1"
                     /translation="MKSANEINQWMTLKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73788..74024
                     /locus_tag="ACHW4Z_000271"
     CDS             73788..74024
                     /locus_tag="ACHW4Z_000271"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8880057.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..75630
                     /locus_tag="ACHW4Z_000272"
                     /pseudo
     CDS             73999..75630
                     /locus_tag="ACHW4Z_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT        18613 a        18994 c        19212 g        18815 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgagg catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 ggttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat ggttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttgag tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat gtataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75481 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75541 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75601 gatccacagc agttacacag cacggtttat tgag
//
LOCUS       DAZSNG010000004        72980 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZSNG010000004 DAZSNG010000000
VERSION     DAZSNG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72980
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.004"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACHW4Z_000273"
     CDS             complement(<1..209)
                     /locus_tag="ACHW4Z_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8880058.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHW4Z_000274"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHW4Z_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR8880059.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHW4Z_000275"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHW4Z_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR8880060.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHW4Z_000276"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHW4Z_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR8880061.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHW4Z_000277"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHW4Z_000277"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR8880062.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHW4Z_000278"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHW4Z_000278"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR8880063.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHW4Z_000279"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHW4Z_000279"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR8880064.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHW4Z_000280"
     CDS             complement(4598..5320)
                     /locus_tag="ACHW4Z_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8880065.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHW4Z_000281"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHW4Z_000281"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8880066.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHW4Z_000282"
     CDS             6520..6738
                     /locus_tag="ACHW4Z_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR8880067.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHW4Z_000283"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHW4Z_000283"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8880068.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHW4Z_000284"
     CDS             complement(7472..7672)
                     /locus_tag="ACHW4Z_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR8880069.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHW4Z_000285"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHW4Z_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHW4Z_000286"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHW4Z_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR8880070.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHW4Z_000287"
     CDS             10101..12014
                     /locus_tag="ACHW4Z_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8880071.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHW4Z_000288"
     CDS             12014..13036
                     /locus_tag="ACHW4Z_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8880072.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHW4Z_000289"
     CDS             13030..13248
                     /locus_tag="ACHW4Z_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR8880073.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHW4Z_000290"
     CDS             13302..14171
                     /locus_tag="ACHW4Z_000290"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR8880074.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHW4Z_000291"
     CDS             complement(14226..14630)
                     /locus_tag="ACHW4Z_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR8880075.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHW4Z_000292"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHW4Z_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR8880076.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHW4Z_000293"
     CDS             15615..17705
                     /locus_tag="ACHW4Z_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR8880077.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHW4Z_000294"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHW4Z_000294"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR8880078.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHW4Z_000295"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHW4Z_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR8880079.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHW4Z_000296"
     CDS             complement(19673..20275)
                     /locus_tag="ACHW4Z_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR8880080.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHW4Z_000297"
     CDS             complement(20265..20432)
                     /locus_tag="ACHW4Z_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR8880081.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHW4Z_000298"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHW4Z_000298"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR8880082.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="ACHW4Z_000299"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="ACHW4Z_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR8880083.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="ACHW4Z_000300"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="ACHW4Z_000300"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR8880084.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="ACHW4Z_000301"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="ACHW4Z_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR8880085.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="ACHW4Z_000302"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="ACHW4Z_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR8880086.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cysG"
                     /locus_tag="ACHW4Z_000303"
     CDS             26649..28022
                     /gene="cysG"
                     /locus_tag="ACHW4Z_000303"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR8880087.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="ACHW4Z_000304"
     CDS             28269..28436
                     /locus_tag="ACHW4Z_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR8880088.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="ACHW4Z_000305"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="ACHW4Z_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="ACHW4Z_000306"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="ACHW4Z_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR8880089.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="ACHW4Z_000307"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="ACHW4Z_000307"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR8880090.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="ACHW4Z_000308"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="ACHW4Z_000308"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR8880091.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="frlR"
                     /locus_tag="ACHW4Z_000309"
                     /gene_synonym="yhfR"
     CDS             32873..33604
                     /gene="frlR"
                     /locus_tag="ACHW4Z_000309"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR8880092.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     GKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="ACHW4Z_000310"
     CDS             complement(33764..34849)
                     /locus_tag="ACHW4Z_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR8880093.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="ACHW4Z_000311"
     CDS             complement(34861..36165)
                     /locus_tag="ACHW4Z_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR8880094.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="ACHW4Z_000312"
     CDS             complement(36177..36530)
                     /locus_tag="ACHW4Z_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR8880095.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="ACHW4Z_000313"
     CDS             complement(36541..37419)
                     /locus_tag="ACHW4Z_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR8880096.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="ACHW4Z_000314"
     CDS             complement(37416..38642)
                     /locus_tag="ACHW4Z_000314"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8880097.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="ACHW4Z_000315"
     CDS             complement(38643..39806)
                     /locus_tag="ACHW4Z_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR8880098.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="ACHW4Z_000316"
     CDS             complement(39890..40252)
                     /locus_tag="ACHW4Z_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR8880099.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /gene="yhfZ"
                     /locus_tag="ACHW4Z_000317"
     CDS             complement(40269..41174)
                     /gene="yhfZ"
                     /locus_tag="ACHW4Z_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR8880100.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="ACHW4Z_000318"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="ACHW4Z_000318"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR8880101.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="ACHW4Z_000319"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="ACHW4Z_000319"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR8880102.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="ACHW4Z_000320"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="ACHW4Z_000320"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR8880103.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="ACHW4Z_000321"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="ACHW4Z_000321"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8880104.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="ACHW4Z_000322"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="ACHW4Z_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR8880105.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="ACHW4Z_000323"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="ACHW4Z_000323"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR8880106.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="ACHW4Z_000324"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="ACHW4Z_000324"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR8880107.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="ACHW4Z_000325"
     CDS             complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="ACHW4Z_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR8880108.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="ACHW4Z_000326"
                     /pseudo
     CDS             complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="ACHW4Z_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="ACHW4Z_000327"
     CDS             complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="ACHW4Z_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR8880109.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50254..50793)
                     /gene="hofN"
                     /locus_tag="ACHW4Z_000328"
     CDS             complement(50254..50793)
                     /gene="hofN"
                     /locus_tag="ACHW4Z_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR8880110.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="ACHW4Z_000329"
     CDS             complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="ACHW4Z_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR8880111.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51692..54244
                     /gene="mrcA"
                     /locus_tag="ACHW4Z_000330"
     CDS             51692..54244
                     /gene="mrcA"
                     /locus_tag="ACHW4Z_000330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR8880112.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="ACHW4Z_000331"
     CDS             complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="ACHW4Z_000331"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR8880113.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55289..57424
                     /gene="igaA"
                     /locus_tag="ACHW4Z_000332"
     CDS             55289..57424
                     /gene="igaA"
                     /locus_tag="ACHW4Z_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR8880114.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57489..58157
                     /gene="yrfG"
                     /locus_tag="ACHW4Z_000333"
     CDS             57489..58157
                     /gene="yrfG"
                     /locus_tag="ACHW4Z_000333"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR8880115.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58168..58569
                     /gene="hslR"
                     /locus_tag="ACHW4Z_000334"
     CDS             58168..58569
                     /gene="hslR"
                     /locus_tag="ACHW4Z_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR8880116.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58594..59463
                     /gene="hslO"
                     /locus_tag="ACHW4Z_000335"
     CDS             58594..59463
                     /gene="hslO"
                     /locus_tag="ACHW4Z_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR8880117.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59607..61358)
                     /locus_tag="ACHW4Z_000336"
     CDS             complement(59607..61358)
                     /locus_tag="ACHW4Z_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFR8880118.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61737..63359
                     /gene="pckA"
                     /locus_tag="ACHW4Z_000337"
     CDS             61737..63359
                     /gene="pckA"
                     /locus_tag="ACHW4Z_000337"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR8880119.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="ACHW4Z_000338"
     CDS             complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="ACHW4Z_000338"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR8880120.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="ACHW4Z_000339"
     CDS             complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="ACHW4Z_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR8880121.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65731..66207
                     /gene="greB"
                     /locus_tag="ACHW4Z_000340"
     CDS             65731..66207
                     /gene="greB"
                     /locus_tag="ACHW4Z_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR8880122.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66304..68625
                     /locus_tag="ACHW4Z_000341"
     CDS             66304..68625
                     /locus_tag="ACHW4Z_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR8880123.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69063..69290
                     /gene="feoA"
                     /locus_tag="ACHW4Z_000342"
     CDS             69063..69290
                     /gene="feoA"
                     /locus_tag="ACHW4Z_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR8880124.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69307..71628
                     /gene="feoB"
                     /locus_tag="ACHW4Z_000343"
     CDS             69307..71628
                     /gene="feoB"
                     /locus_tag="ACHW4Z_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR8880125.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71628..71864
                     /gene="feoC"
                     /locus_tag="ACHW4Z_000344"
     CDS             71628..71864
                     /gene="feoC"
                     /locus_tag="ACHW4Z_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR8880126.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72067..72954
                     /gene="rpnA"
                     /locus_tag="ACHW4Z_000345"
     CDS             72067..72954
                     /gene="rpnA"
                     /locus_tag="ACHW4Z_000345"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR8880127.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17590 a        19778 c        18456 g        17156 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatggca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgccgggtaa gattagcgta
    48121 aaaaagacag caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa
    48181 gagcaccaac tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga
    48241 tgaaacacgt tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca
    48301 gtcgtggcgt gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa
    48361 ataattgccc gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat
    48421 ttttacgggt aaaaatgccg cccagcgcca acgtttctcc gctttttacc tcgacctgcg
    48481 tttcgatctc ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc
    48541 ccggaacgtt ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg
    48601 gcgtgacctc catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc
    48661 cgctggaaac ctgatatgga atttcgctct cctgtttaat gctggcaggc tgaagatgtg
    48721 aggccagcag acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa
    48781 gctcaagatc cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg
    48841 ccgtcgctac ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt
    48901 gcgcatcggc cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga
    48961 caatatgcgc cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt
    49021 caagcgcgct taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga
    49081 cggtcatact ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt
    49141 cggcgtattg cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct
    49201 gctccgcctc ctggcgggcg atattgtcat tctgccaggc aatggaatgc actgagagaa
    49261 tgttgccttc ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct
    49321 tccagggaac atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca
    49381 ggttcaactt ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca
    49441 ccatcagcgt cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta
    49501 gtgcggctat ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt
    49561 tcgcagtttg tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag
    49621 ccgttttcca gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt
    49681 acaccgatga tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg
    49741 ctaatccggc atatagatct tccggcgatt taaaagggtc acgcataccg gttaaaaggc
    49801 acaatgcaat acctgccaac aaccagcgtt taaccctcat gcggcatctc cagttgtagc
    49861 gtgaacaaaa gatcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc
    49921 tctgccagcc gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactca
    49981 cctccctgcg cggatggatg ccaggaaacc agttgcgcgc cggataactg aaaatccagt
    50041 ggcgaaaagg gcagcgtttt ttcctcgctg aagggagtgg tgtctaccag gcgatacagt
    50101 gcggcccact gctggtgatg acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg
    50161 tgtgtcgatg atagaaaaat gagcgtaact aacatcagca accagaatgc ccagcaaaac
    50221 tggcggaggc ggggtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt
    50281 ccttgttaac tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggctccccg
    50341 ctgattgaga tgaaaagacg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt
    50401 aatgcttgtt gtcagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag
    50461 ccaggcgtgc tcgggtaaaa gcgccgccag tgcttccagc gcagattgcc agtcgcgggt
    50521 aaattgtcgc tggcgctgcc gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa
    50581 acgtggcttc gttatctgta agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc
    50641 gtctatgcgc gcttcggcgc tgcctgtcag acgcagtatt agcgttatcc cgacggccag
    50701 cagcagaggc gcaacgaaca tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg
    50761 tcgccagggc aaaaaattaa ttggcggatt catcagtacg ccttcccaag cgccagcccc
    50821 agcgcgatgg caaagtctcc accaggcggc ggtagcggcg gctgacgaac agaaacggcc
    50881 tcccaggggt caaatccgcc ttcgccacat atcgcgacgc tgtcgggatc aacggataac
    50941 gctgccgcca gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagctatag
    51001 cgcgtcgccc acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt
    51061 aaaaaaggca ggaatcgctg taatgcactg gcatccgggg tgatcgcact aacatgaaca
    51121 cgcaaccttt ctgccagcgt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg
    51181 ttataagcgg ggctgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata
    51241 tccagctcgc gggccatcgt ccctgacagc caggccgttt gctcccgctc accaagggac
    51301 atcgacgggc gcggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg
    51361 tgatgacgct gcggcagttc gcgactccac ggtaacaacg ttttagccag ctgctgcgca
    51421 tcaacaatcc gcccatcttt gataatgtcg tgcgccagcg gcaaccgcca ccagcgttgc
    51481 aaaaagcatt cttttgcgcc ccgcacgatc gcaaccgctg ccgcttcttg ctgttgtaaa
    51541 tgcaaaccaa tttgccagat cttaaatgtc attgtgatga tctccttatc acccgtcact
    51601 ctgacgggta tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac
    51661 tgcccaaatg aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct
    51721 tgcagtctgt tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga
    51781 gccacaactg ccggatgtgg tgacattaaa agatgttcgc ctgcaaattc cgatgcagat
    51841 ttacagcgcc gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac
    51901 gttggatcaa atcccaccgg agatggtgaa agcctttatc gcgacggaag acagccgctt
    51961 ctacgagcat cacggcgttg acccggtggg gatcttccgt gcggcaagtg tggcgctgtt
    52021 ctccggccac gcgtcacaag gggcgagtac cattacccag cagctggcga gaaatttctt
    52081 cctcagtcca gaacgcacga tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat
    52141 tgaacagttg ctgacgaaag acgaaatcct cgagctttat ctgaacaaga tttaccttgg
    52201 ttaccgcgcc tatggtgtcg gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca
    52261 actgacgctg aacgaaatgg cggtgatagc cgggttgccg aaagcgcctt ccacctttaa
    52321 tccgctctac tcaatggatc gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct
    52381 ggatgaaggg tatatcaccc agcaacagtt cgatcagaca cgcactgagg cgattaacgc
    52441 taattatcac gcgccggaga ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca
    52501 ggagatgtat aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac
    52561 catcacccgc aaagtgcagc aggccgcgca gcaggcggta cgtaataacg tgctggacta
    52621 cgacatgcgc cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc
    52681 gtgggataac aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct
    52741 gcctgccgca gtcaccagcg ccaatcctca gggagcgacg gcgatgctgg cggacgggtc
    52801 gaccgtcgca ttgagtatgg aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca
    52861 gcagggaccg acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt
    52921 tcgtcaggtt ggcgatgcat ggtggctggc acaagtgccg gaagtgaact cggcgctggt
    52981 gtcgatcaat ccgcaaaatg gtgccgttat ggcgctggtc ggtggctttg atttcaatca
    53041 gagcaagttt aaccgcgcca cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt
    53101 cctctacacc gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc
    53161 aatttctcgc tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca
    53221 gtatgctggt ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt
    53281 acgtgcaatg cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt
    53341 cccggcgcaa aacattgtcc acaccgaatc gctggcgctg ggttcagcgt ccttcacccc
    53401 aatgcaggtg gcgcgcggct acgcggtcat ggcgaacggc ggcttcctag tggacccgtg
    53461 gtttatcagc aaaattgaaa acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt
    53521 agcctgcccg gaatgcgata ttccagtgat ttacggtgat acgcagaaat cgaacgtgct
    53581 ggaaaataac gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc
    53641 aatgccgcag ctggagcagg caaatcaggc gttagtggcg aagactggcg cgcaggagta
    53701 cgcaccgcac gtcatcaaca ctccgctggc attcctgatt aagagtgctt tgaacaccaa
    53761 tatctttggt gaaccaggct ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg
    53821 tcgcgatatc ggcgggaaaa ccggtaccac taacagttcg aaagatgcgt ggttctcggg
    53881 ttacggtccg ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct
    53941 cggtcataca acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc
    54001 caagagtgcc cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtgccgga
    54061 gcagccgctg acgccgcctc cgggtattgt gacggtgaat atcgatcgca gcaccgggca
    54121 gttagctaat ggtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca
    54181 acaggcggtg cacgaggtgg gaacgaccat tatcgataat ggtgaggcac aggaattgtt
    54241 ctgattaaaa aggcgcttcg gcgccttttc agttggctga caaagtgcat ttgtttatgc
    54301 cggatgcggg tgaacgcgtt atccggctaa caaaatcgtg aaaaatcaat atattgcagg
    54361 aaccccataa gcctgataag cgttgtgcat caggcaaact tcacgcattt acactcgccc
    54421 ctgccctttc aaccattcgc gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa
    54481 gtcagggtct tccagcaaat ccatcatatg cgccagcggc cagcgcacct gtggtagcgg
    54541 ctctggctca tcgccttcca gcgattccgg gtagagatct tgcgcgacca cgatattcat
    54601 tttgctggaa aagtaagacg gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc
    54661 accaaatcca acctcttctt ttagctcgcg gttagcggct tcgtagacgc tttcacccgg
    54721 atcaattaat cctttcgaaa aacctaattc gtaggattca gttcccactg cgtattcgcg
    54781 gatcaggatc aggtgatcgt ccacaatcgg cacaatcatc actgcttccc ggttggttgg
    54841 acgcattcgt tcataaacac gccgcacgcc attgctgaac tccagatcca cgctctcgac
    54901 ggtaaacagt cgggaacggg ctacagtttc aacattcaga atggtgggtt tttgtaatga
    54961 tttgttcatc gtgggatcta tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc
    55021 tttcggcaat gtgaattgca tgttatttac atttatgtaa cttaataaat aatcgtcctc
    55081 aaatcaaatt aaaagtcaat aggttgaaat aactccagga atttgctgat attccgcctt
    55141 cagagggttt gctatgatca gcggttactg tgatgtgctt aatgatgctc aagttaagct
    55201 ccacgcttgc cgatagccaa ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc
    55261 ctgacagact aagtaagatg gggaaagcat gagcaccatt gtgatttttt tagctgcttt
    55321 gctggcctgc tcactacttg cgggatggct gataaaagtg cgatccagac ggcgtcagct
    55381 gccctggacc aacgccttcg cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag
    55441 cgccgttgaa aattatcttg agagcctgac gcaggtatta caggtgcctg gcccaacgga
    55501 agctagcgcg gcaccgatct ctctggcgct gaatgccgaa agcaacaacg tcatgatgct
    55561 gacacacgct atcacacgtt acggcatctc taccgacgat ccgaataaat ggcgttacta
    55621 cctcgattcg gtagaagtcc acctgccccc cttctgggaa cagtacatca acgatgagaa
    55681 taccgttgaa ctgattcata ccgattcgct gccgttggtt atttcactca acggtcatac
    55741 gctgcaggag tacatgcagg aaacgcgcgg ctatgccttg caacctgttc cgtccacgca
    55801 ggcgtcgatt cgcggggaag agagtgagca aatcgagcta ctcaatattc gcaaagaaac
    55861 gcatgaagaa tatgcgctga gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc
    55921 ctccttcctg atgttctttt tctgcctgat taccccggat gtatttgttc cgtggctggc
    55981 aggcggcgca ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa
    56041 atcctccctg cgagaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg
    56101 cgaaaacgat caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc
    56161 cgcacactgg cagccataca ttgctcagga tctcggtcaa caaaccgata ttgatatcta
    56221 tctcgaccgc cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa
    56281 ctttccgtta cagcactggc tgcgcagtac gattatcgct gcgggctcgc tgctagtgct
    56341 gtttatgctg ttattctgga tcccgctgga tatgccgctg aaattcactc tctcatggat
    56401 gaaaggcgcg cagaccattg aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg
    56461 ggtaggcgat acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggaac
    56521 ctggagcgcg aaaaccaatt cacctttttt accgtttgac tgctcgcaga tcatctggaa
    56581 tgacgcccgc tcattaccat taccagaatc tgaactggtc aacaaagcga cggcattgac
    56641 tgaagcagtt aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc
    56701 attacgttca gcgattcaaa aatccggcat ggtattgctt gatgattttg gcgacattgt
    56761 actgaagaca gcggatttat gttctgccaa agatgactgt gtgcgactga aaaatgcgct
    56821 ggtcaatctc ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa
    56881 gctcgatggc gtgaatgtgt tattacgccc ggtgagtgct gaatcgctgg ataacctggt
    56941 ggcaacctcc accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa
    57001 cagtccggcc cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca
    57061 gccctggcct tcggcatcac tttacgacta cccgccgcaa gaacagtgga acgctttcca
    57121 gaaactggca caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat
    57181 cttcactgac gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg
    57241 cctgtggcgc tatctcagca ccacattgct gctactgacg atgctgggta gcgccattta
    57301 caatggcgta caggcctggc gtcgttacca gcgtcatcgc actcgcatga tggagattca
    57361 ggcctattat gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga
    57421 ataacacgtt tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga
    57481 tttccctcat gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg
    57541 acggcacgtt gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa
    57601 catggggcgc gaaaaacggg gttacgccac aggaagcgat ggaatatatg cgccagcaat
    57661 atcacgacgt acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg
    57721 gtctggatat ctgtgcgatg accaccgaga tgggaccgcg cgccgtactg cgtgaagata
    57781 ccattccgtt tcttgaggca ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg
    57841 ctcatccgca caacctggcg gtaaaacttg agcatacggg tctggacgca caccttgatt
    57901 tattactttc cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg
    57961 tggccgaagc tacgggtctg aaagctgaaa gaacgctgtt tattgatgac agcgaagcga
    58021 ttctcgatgc tgccgcgcaa tttggtattc gttactgcct cggcgtgact aatcctgatt
    58081 ccgggattgc cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga
    58141 tcccctcgct aatgtgaagg agacgccatg aaagagaaac ctgctgttga ggttcgactg
    58201 gataaatggc tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt
    58261 gaaggcggta aggtgcatta caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg
    58321 aatgccacgc tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt
    58381 actgaacagc gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt
    58441 gtagagaaac gcgaaaaaat ggcgatggca cgtaaactta atgccttaac catgccgcac
    58501 ccggaccgac gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac
    58561 agtgaataac tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc
    58621 tatctgtttg aaaattttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa
    58681 cagatccttg agaaccacga ttatccgcag cccgttaaaa acgtgctggc agaactgctg
    58741 gttgcgacca gcctgttaac cgctacgctg aagtttgatg gtgatatcac cgtacagctg
    58801 cagggcgacg gtccgatgaa tctggcggtt attaacggta acaataacca gcaaatgcgc
    58861 ggtgtggcgc gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc
    58921 aatggttacg tggtgatcac cattaccccg agcgaaggcg aacgctatca gggcgtagtt
    58981 ggtctggaag gtgataccct ggcggcctgc ctagaagatt actttatgcg ttctgaacag
    59041 ctgccgacgc gcctgtttat tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt
    59101 atgttgttgc aggtaatgcc tgcgcaaaat acccaacagg acgactttga ccacctggcg
    59161 acgctaaccg aaaccatcaa aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg
    59221 tggcgtttgt atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa
    59281 tgcacctgct cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt
    59341 gatagcatcc tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac
    59401 tatctgttca atgcgatgga tattgctgaa atccgcaaca acgcgtctcc ggcagatccg
    59461 taagttcatt aatgagtatg tccggcagag agggctctgc cggattttac gacttaatgg
    59521 cctgatgtgc tgcgcttacc agcgcctatg aaataaatcg caaattattt tggtcagata
    59581 cagcgtcgta tccgataaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca
    59641 tctaccgtca gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct
    59701 gtgagtaatt tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat
    59761 tcttttctgt ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg
    59821 atccgctccg cctgaccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt
    59881 tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc
    59941 ggtttaacag aaccaggggc aattaacaca ttttcggcga atactttttc cgatacctgt
    60001 tcctgaagat gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct
    60061 ttcataaacc cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc
    60121 tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc
    60181 atatgactgt tcacgctaat acgcatactg tccagcaccg gcgaattaag aagcaccagt
    60241 atcaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt
    60301 tgcccattac gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc
    60361 tccgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat
    60421 aacccccagg tggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg
    60481 caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt
    60541 aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa
    60601 atcgcgctca ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact
    60661 aacggcagca accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt
    60721 gactgtgtac gcgcgaggat caccgccagt gcggtaacca gaccaaatgt aagatacata
    60781 aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac
    60841 tcaccccaca gtaaaagcac cagccacgtc aggccattgg agagaaaaat caccagtaat
    60901 ataagtacgt tatgccatac tgactggtaa aagtagctat agcggctact gccattgcgg
    60961 atacgaaggc tttgttgtat ccacggtagc aacaacattg ccatcaacag cagatagcag
    61021 ccaaaatccc aaatcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc
    61081 agccaaccgc tcatccctgt cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc
    61141 ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc
    61201 acgccataaa caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa
    61261 accagcccct gaagtaatcc ggtagcaatc atcccctgaa gtaatccggt agcaatcatc
    61321 ccccagcgtg ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag
    61381 gtgggttaat tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa
    61441 aagttagcgt ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat
    61501 gcgattccac tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt
    61561 tccacaccta aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga
    61621 gttttttgtc aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa
    61681 cacccccaaa aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc
    61741 gcgttaacaa tggtttgccc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg
    61801 atatcgttta caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga
    61861 caggttatga gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct
    61921 tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt
    61981 tctggtgggc agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct
    62041 ggcagcatct gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg
    62101 acgctttctg tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg
    62161 cctggcaggc gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag
    62221 gtttcaaacc agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag
    62281 aacagggtct gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga
    62341 ttggcggcac ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc
    62401 tgctgccgct gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg
    62461 atgttgcggt gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga
    62521 aacgccgcct gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaatttcg
    62581 aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca
    62641 acgctatccg tcgtgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg
    62701 actttgatga tggttcaaaa accgagaaca cccgcgtttc ttatccaatc tatcacatcg
    62761 ataacatcgt taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg
    62821 ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc
    62881 acttcctctc tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga
    62941 cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg
    63001 cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg
    63061 gctggaacgg cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca
    63121 tcctcaacgg ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga
    63181 tcccaaccga actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt
    63241 ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg
    63301 ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat
    63361 gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt
    63421 cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc
    63481 agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta
    63541 tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc
    63601 gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc
    63661 ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg
    63721 atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc
    63781 gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc
    63841 tcatagccac tttcggcagc aatcacctca ccgagcactg cattaagatc cgccatttcc
    63901 atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac
    63961 tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca
    64021 gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc
    64081 agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta
    64141 acggaacgca cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttacca
    64201 acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc
    64261 cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa
    64321 tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt
    64381 ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc
    64441 agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc
    64501 tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg
    64561 aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac
    64621 atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag
    64681 ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat
    64741 aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt
    64801 ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat
    64861 cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg
    64921 aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat
    64981 ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt
    65041 cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct
    65101 cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac
    65161 gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca
    65221 ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt
    65281 tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat
    65341 ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag
    65401 cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc
    65461 gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag
    65521 gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat
    65581 ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct taagtataca
    65641 atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc
    65701 attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg
    65761 tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca
    65821 aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat
    65881 aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa
    65941 aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg
    66001 gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa
    66061 atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa
    66121 aaagaagtcg gcgatctggc ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt
    66181 aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca
    66241 tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa
    66301 accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa
    66361 caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt
    66421 tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt
    66481 ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc catttccgag
    66541 caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa
    66601 ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt
    66661 gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa
    66721 gtcgccgctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg
    66781 gatggcgcgc gctatatcct gatggaacgg tttgccgaag atgccacgct gctggcgaaa
    66841 gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa
    66901 gaggaagggg cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct
    66961 tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg
    67021 aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg
    67081 gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg
    67141 agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg
    67201 cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg
    67261 atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg
    67321 gtaaaagtgg cggtggtcga tgccactggc aaactggtgg cgaccgatac catttacccg
    67381 cacaccggac aagccgcaaa agcagcgatg accgttgctg ccttgtgtga aaaacataac
    67441 gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaactgaacg tttctatctc
    67501 gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc
    67561 gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg
    67621 ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa
    67681 atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg
    67741 gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac
    67801 accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac
    67861 atcgtggcct ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc
    67921 agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt
    67981 gataacccgc tggacgcctc taccgttcat ccggaagcct atccggtggt ggaacgcatt
    68041 ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac
    68101 ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc
    68161 aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc
    68221 gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc
    68281 aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac
    68341 atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac
    68401 attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg
    68461 cgtctggacg agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg
    68521 caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc
    68581 gcgatgatgg atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc
    68641 cctaaacctg cgcgataagg cgttcacgcc gcatctggca acgaacgctt tgcctgacat
    68701 aaaagtgccg gagaatatct ccggcacttt tattccacag ccaaactcac aatatactcc
    68761 gttaatattt atcacttcat taacaactga aaccttaatt aaacattagc cagtccgggt
    68821 aactcactat tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag
    68881 tcagattatt attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt
    68941 ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat catctggtct
    69001 catgtcgcgg tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac
    69061 ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc
    69121 cggcatatcg ccaaaaactg ctttctctag gcatgttacc tggctcctct tttgatgtgg
    69181 tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat
    69241 tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata
    69301 acaacaatga aaaaattaac cattggctta attggtaatc caaattctgg caagacaacc
    69361 ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc
    69421 gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc
    69481 ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt
    69541 cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt
    69601 gagcgtaacc tgtacctgac gctgcaactg ctggaactcg gcattccctg catcgtggcg
    69661 ctgaacatgc tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctatcg
    69721 gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg
    69781 ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca
    69841 cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg
    69901 aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac
    69961 gccggtgaag cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa tgaaatggat
    70021 gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg
    70081 gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg
    70141 ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg
    70201 gctatcaaca tcggcggggc gttacagccg ctgtttaacg tcggctccgt ggcgctattt
    70261 gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc
    70321 ctcgcccagg gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc
    70381 atgatgtacc tgttcctctc cttccttgag gactccggct atatggcgcg tgcagcgttt
    70441 gtgatggacc gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc
    70501 gtcggtttcg gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt
    70561 gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc
    70621 ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg ccctggcggt tttctcgctg
    70681 tatatgctcg gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg
    70741 cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa
    70801 agcctgatta tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg
    70861 attatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc
    70921 gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgataac ccctgtcttc
    70981 aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt tacgggcgca
    71041 atggcaaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag
    71101 gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag tgcggtagat
    71161 gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa
    71221 gctagcaaag gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc
    71281 ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc
    71341 tcggtgatgg gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg
    71401 tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc cagctacagt
    71461 cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct
    71521 atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag
    71581 tcggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta
    71641 ttcaggtacg tgatttgctg gcgttacggg gccgtatgga agcgacccag ataagccaga
    71701 cattgaacac tccgcagcca atgattaacg ccatgctgca acagctggaa agcatgggca
    71761 aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc
    71821 cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct
    71881 gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa
    71941 aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact
    72001 cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg
    72061 aacgccatga gtaaaaagca gagttccacc ccacatgatg cgctgttcaa actcttttta
    72121 cgccaaccgg agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg
    72181 ctctgtgata tgaaaaccct caagctggag tcgaggagct ttattgatga cgatctgcgt
    72241 gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat
    72301 tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac
    72361 gctattgccg caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg
    72421 ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat
    72481 tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat
    72541 gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta
    72601 attcaaaaac atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta
    72661 ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa
    72721 actggcgacg ctgtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa
    72781 cacaaggaga gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa
    72841 gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcga
    72901 tttaccgggc tgtcagaaga agagttggct gcggcgagta gactggcccc ctgaatctcc
    72961 agacaaccag tatcacttaa
//
LOCUS       DAZSNG010000005        70712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZSNG010000005 DAZSNG010000000
VERSION     DAZSNG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.005"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..795
                     /locus_tag="ACHW4Z_000346"
     CDS             <1..795
                     /locus_tag="ACHW4Z_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8880128.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHW4Z_000347"
     CDS             806..1897
                     /locus_tag="ACHW4Z_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8880129.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHW4Z_000348"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHW4Z_000348"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR8880130.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHW4Z_000349"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHW4Z_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR8880131.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHW4Z_000350"
     CDS             4859..5254
                     /locus_tag="ACHW4Z_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR8880132.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHW4Z_000351"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHW4Z_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR8880133.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHW4Z_000352"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHW4Z_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR8880134.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHW4Z_000353"
     CDS             complement(6563..7603)
                     /locus_tag="ACHW4Z_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8880135.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHW4Z_000354"
     CDS             complement(7676..8311)
                     /locus_tag="ACHW4Z_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR8880136.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHW4Z_000355"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHW4Z_000355"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR8880137.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHW4Z_000356"
     CDS             complement(8937..9380)
                     /locus_tag="ACHW4Z_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR8880138.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHW4Z_000357"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHW4Z_000357"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR8880139.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHW4Z_000358"
     CDS             complement(9720..10223)
                     /locus_tag="ACHW4Z_000358"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8880140.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHW4Z_000359"
     CDS             complement(10217..10741)
                     /locus_tag="ACHW4Z_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR8880141.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHW4Z_000360"
     CDS             10950..11945
                     /locus_tag="ACHW4Z_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR8880142.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHW4Z_000361"
     CDS             11954..12832
                     /locus_tag="ACHW4Z_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR8880143.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHW4Z_000362"
     CDS             12913..13920
                     /locus_tag="ACHW4Z_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR8880144.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHW4Z_000363"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHW4Z_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR8880145.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHW4Z_000364"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHW4Z_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR8880146.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHW4Z_000365"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHW4Z_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR8880147.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHW4Z_000366"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHW4Z_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR8880148.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHW4Z_000367"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHW4Z_000367"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR8880149.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHW4Z_000368"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHW4Z_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR8880150.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHW4Z_000369"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHW4Z_000369"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR8880151.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHW4Z_000370"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHW4Z_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR8880152.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHW4Z_000371"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHW4Z_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR8880153.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHW4Z_000372"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHW4Z_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR8880154.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHW4Z_000373"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHW4Z_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR8880155.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHW4Z_000374"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHW4Z_000374"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHW4Z_000375"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHW4Z_000375"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR8880156.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHW4Z_000376"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHW4Z_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHW4Z_000377"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHW4Z_000377"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHW4Z_000378"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHW4Z_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR8880157.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHW4Z_000379"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHW4Z_000379"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR8880158.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHW4Z_000380"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHW4Z_000380"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR8880159.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHW4Z_000381"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHW4Z_000381"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR8880160.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHW4Z_000382"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHW4Z_000382"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR8880161.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWLKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHW4Z_000383"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHW4Z_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR8880162.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHW4Z_000384"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHW4Z_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR8880163.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHW4Z_000385"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHW4Z_000385"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8880164.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHW4Z_000386"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHW4Z_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHW4Z_000387"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHW4Z_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR8880165.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHW4Z_000388"
     CDS             complement(41212..42177)
                     /locus_tag="ACHW4Z_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8880166.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHW4Z_000389"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHW4Z_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR8880167.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHW4Z_000390"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHW4Z_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR8880168.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHW4Z_000391"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHW4Z_000391"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR8880169.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHW4Z_000392"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHW4Z_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR8880170.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHW4Z_000393"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHW4Z_000393"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR8880171.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHW4Z_000394"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHW4Z_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR8880172.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHW4Z_000395"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHW4Z_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR8880173.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHW4Z_000396"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHW4Z_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR8880174.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHW4Z_000397"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHW4Z_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR8880175.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHW4Z_000398"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHW4Z_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR8880176.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHW4Z_000399"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHW4Z_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR8880177.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHW4Z_000400"
     CDS             49715..50680
                     /locus_tag="ACHW4Z_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR8880178.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHW4Z_000401"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHW4Z_000401"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR8880179.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHW4Z_000402"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHW4Z_000402"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR8880180.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHW4Z_000403"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHW4Z_000403"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR8880181.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHW4Z_000404"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHW4Z_000404"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR8880182.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHW4Z_000405"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHW4Z_000405"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR8880183.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHW4Z_000406"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHW4Z_000406"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR8880184.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHW4Z_000407"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHW4Z_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR8880185.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHW4Z_000408"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHW4Z_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR8880186.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHW4Z_000409"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHW4Z_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR8880187.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHW4Z_000410"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHW4Z_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR8880188.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHW4Z_000411"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHW4Z_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR8880189.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHW4Z_000412"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHW4Z_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHW4Z_000413"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHW4Z_000413"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR8880190.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHW4Z_000414"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHW4Z_000414"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR8880191.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHW4Z_000415"
     CDS             complement(62547..63476)
                     /locus_tag="ACHW4Z_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR8880192.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHW4Z_000416"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHW4Z_000416"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR8880193.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHW4Z_000417"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHW4Z_000417"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR8880194.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHW4Z_000418"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHW4Z_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17313 a        19176 c        17561 g        16662 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaagcc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZSNG010000006        67672 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZSNG010000006 DAZSNG010000000
VERSION     DAZSNG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67672
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.006"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..229
                     /locus_tag="ACHW4Z_000419"
     CDS             <1..229
                     /locus_tag="ACHW4Z_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR8880195.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="ACHW4Z_000420"
                     /pseudo
     CDS             624..1263
                     /locus_tag="ACHW4Z_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="ACHW4Z_000421"
     CDS             1366..1689
                     /locus_tag="ACHW4Z_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR8880196.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="ACHW4Z_000422"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="ACHW4Z_000422"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR8880197.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="ACHW4Z_000423"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="ACHW4Z_000423"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR8880198.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="ACHW4Z_000424"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="ACHW4Z_000424"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR8880199.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="ACHW4Z_000425"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="ACHW4Z_000425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR8880200.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="ACHW4Z_000426"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="ACHW4Z_000426"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR8880201.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="ACHW4Z_000427"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="ACHW4Z_000427"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR8880202.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="ACHW4Z_000428"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="ACHW4Z_000428"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR8880203.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="ACHW4Z_000429"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="ACHW4Z_000429"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR8880204.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="ACHW4Z_000430"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="ACHW4Z_000430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR8880205.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="ACHW4Z_000431"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="ACHW4Z_000431"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR8880206.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="ACHW4Z_000432"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="ACHW4Z_000432"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR8880207.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="ACHW4Z_000433"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="ACHW4Z_000433"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR8880208.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="ACHW4Z_000434"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="ACHW4Z_000434"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR8880209.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="ACHW4Z_000435"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="ACHW4Z_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR8880210.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="ACHW4Z_000436"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="ACHW4Z_000436"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR8880211.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="ACHW4Z_000437"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="ACHW4Z_000437"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR8880212.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="ACHW4Z_000438"
     CDS             complement(21227..23059)
                     /locus_tag="ACHW4Z_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR8880213.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="ACHW4Z_000439"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="ACHW4Z_000439"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR8880214.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="ACHW4Z_000440"
     CDS             complement(23807..24301)
                     /locus_tag="ACHW4Z_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR8880215.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /gene="yfbV"
                     /locus_tag="ACHW4Z_000441"
     CDS             complement(24384..24839)
                     /gene="yfbV"
                     /locus_tag="ACHW4Z_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR8880216.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="ACHW4Z_000442"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="ACHW4Z_000442"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR8880217.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="ACHW4Z_000443"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="ACHW4Z_000443"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR8880218.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="ACHW4Z_000444"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="ACHW4Z_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR8880219.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="ACHW4Z_000445"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="ACHW4Z_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR8880220.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="ACHW4Z_000446"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="ACHW4Z_000446"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR8880221.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="ACHW4Z_000447"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="ACHW4Z_000447"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR8880222.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="ACHW4Z_000448"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="ACHW4Z_000448"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR8880223.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="ACHW4Z_000449"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="ACHW4Z_000449"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR8880224.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="ACHW4Z_000450"
     CDS             33415..34308
                     /locus_tag="ACHW4Z_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR8880225.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="ACHW4Z_000451"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="ACHW4Z_000451"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR8880226.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="ACHW4Z_000452"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="ACHW4Z_000452"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR8880227.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="ACHW4Z_000453"
     CDS             complement(36224..36940)
                     /locus_tag="ACHW4Z_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8880228.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="ACHW4Z_000454"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="ACHW4Z_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR8880229.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="ACHW4Z_000455"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="ACHW4Z_000455"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR8880230.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="ACHW4Z_000456"
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="ACHW4Z_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR8880231.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="ACHW4Z_000457"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="ACHW4Z_000457"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR8880232.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="ACHW4Z_000458"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="ACHW4Z_000458"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR8880233.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="ACHW4Z_000459"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="ACHW4Z_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR8880234.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="ACHW4Z_000460"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="ACHW4Z_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR8880235.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="ACHW4Z_000461"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="ACHW4Z_000461"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR8880236.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="ACHW4Z_000462"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="ACHW4Z_000462"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR8880237.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="ACHW4Z_000463"
     CDS             complement(45789..46448)
                     /locus_tag="ACHW4Z_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8880238.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="ACHW4Z_000464"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="ACHW4Z_000464"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR8880239.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="ACHW4Z_000465"
     CDS             complement(47343..48356)
                     /locus_tag="ACHW4Z_000465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8880240.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="ACHW4Z_000466"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="ACHW4Z_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR8880241.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="ACHW4Z_000467"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="ACHW4Z_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR8880242.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="ACHW4Z_000468"
     CDS             complement(50649..51827)
                     /locus_tag="ACHW4Z_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8880243.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="ACHW4Z_000469"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="ACHW4Z_000469"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR8880244.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="ACHW4Z_000470"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="ACHW4Z_000470"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR8880245.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="ACHW4Z_000471"
     CDS             complement(55609..55887)
                     /locus_tag="ACHW4Z_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR8880246.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="ACHW4Z_000472"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="ACHW4Z_000472"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR8880247.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="ACHW4Z_000473"
     CDS             complement(56469..57278)
                     /locus_tag="ACHW4Z_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR8880248.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="ACHW4Z_000474"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="ACHW4Z_000474"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR8880249.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="ACHW4Z_000475"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="ACHW4Z_000475"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR8880250.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="ACHW4Z_000476"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="ACHW4Z_000476"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8880251.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="ACHW4Z_000477"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="ACHW4Z_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR8880252.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="ACHW4Z_000478"
     CDS             complement(60979..61869)
                     /locus_tag="ACHW4Z_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR8880253.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="ACHW4Z_000479"
     CDS             complement(61856..62380)
                     /locus_tag="ACHW4Z_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8880254.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="ACHW4Z_000480"
     CDS             complement(62377..62847)
                     /locus_tag="ACHW4Z_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8880255.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="ACHW4Z_000481"
     CDS             complement(62844..63392)
                     /locus_tag="ACHW4Z_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8880256.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="ACHW4Z_000482"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="ACHW4Z_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..66779)
                     /gene="yfcU"
                     /locus_tag="ACHW4Z_000483"
                     /pseudo
     CDS             complement(64139..66779)
                     /gene="yfcU"
                     /locus_tag="ACHW4Z_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66861..67424)
                     /locus_tag="ACHW4Z_000484"
     CDS             complement(66861..67424)
                     /locus_tag="ACHW4Z_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8880257.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16925 a        18283 c        16784 g        15680 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaatga
      601 cgccttttat tgataattta catatgaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aagcctcatt atttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaagaa acatacaacg catgatacat atccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32521 aaccgttaag cctctttgag gctgctgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgagaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc agaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttacggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca atatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
    66121 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
    66181 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
    66241 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
    66301 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
    66361 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
    66421 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
    66481 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
    66541 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
    66601 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
    66661 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
    66721 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
    66781 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
    66841 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
    66901 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
    66961 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
    67021 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
    67081 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
    67141 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
    67201 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
    67261 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
    67321 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
    67381 atcaccattg ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact
    67441 ataaataatt aaaaatataa acattgcaat acattgattc agtcaatagc caatgtttat
    67501 acccagccat tgaatacggc atgcgaccca aaaagaacag gtcgtagaaa attgtattca
    67561 ttggtgtggg gccgcatagt gatgtttcca cacccctgat ccaatttaat tttactaaag
    67621 agtaaatgta gatttaatta atatattgac agaagaaagg ttatcttaag ga
//
LOCUS       DAZSNG010000007        67022 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZSNG010000007 DAZSNG010000000
VERSION     DAZSNG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67022
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.007"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="ACHW4Z_000485"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="ACHW4Z_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR8880258.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="ACHW4Z_000486"
     CDS             complement(1151..1237)
                     /locus_tag="ACHW4Z_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR8880259.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="ACHW4Z_000487"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="ACHW4Z_000487"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8880260.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="ACHW4Z_000488"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="ACHW4Z_000488"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR8880261.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="ACHW4Z_000489"
     CDS             3586..3630
                     /locus_tag="ACHW4Z_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880262.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="ACHW4Z_000490"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="ACHW4Z_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR8880263.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="ACHW4Z_000491"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="ACHW4Z_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR8880264.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="ACHW4Z_000492"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="ACHW4Z_000492"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR8880265.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="ACHW4Z_000493"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="ACHW4Z_000493"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR8880266.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="ACHW4Z_000494"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="ACHW4Z_000494"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR8880267.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="ACHW4Z_000495"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="ACHW4Z_000495"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR8880268.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="ACHW4Z_000496"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="ACHW4Z_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR8880269.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="ACHW4Z_000497"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="ACHW4Z_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR8880270.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="ACHW4Z_000498"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="ACHW4Z_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR8880271.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWLEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="ACHW4Z_000499"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="ACHW4Z_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR8880272.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="ACHW4Z_000500"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="ACHW4Z_000500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR8880273.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="ACHW4Z_000501"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="ACHW4Z_000501"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR8880274.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="ACHW4Z_000502"
     CDS             complement(16981..17745)
                     /locus_tag="ACHW4Z_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8880275.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="ACHW4Z_000503"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="ACHW4Z_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR8880276.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="ACHW4Z_000504"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="ACHW4Z_000504"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR8880277.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="ACHW4Z_000505"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="ACHW4Z_000505"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR8880278.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="ACHW4Z_000506"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="ACHW4Z_000506"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR8880279.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="ACHW4Z_000507"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="ACHW4Z_000507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR8880280.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="ACHW4Z_000508"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="ACHW4Z_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR8880281.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="ACHW4Z_000509"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="ACHW4Z_000509"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR8880282.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="ACHW4Z_000510"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="ACHW4Z_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR8880283.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="ACHW4Z_000511"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="ACHW4Z_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR8880284.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="ACHW4Z_000512"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="ACHW4Z_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR8880285.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="ACHW4Z_000513"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="ACHW4Z_000513"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR8880286.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="ACHW4Z_000514"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="ACHW4Z_000514"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR8880287.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="ACHW4Z_000515"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="ACHW4Z_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR8880288.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="ACHW4Z_000516"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="ACHW4Z_000516"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR8880289.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="ACHW4Z_000517"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="ACHW4Z_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR8880290.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="ACHW4Z_000518"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="ACHW4Z_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR8880291.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="ACHW4Z_000519"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="ACHW4Z_000519"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR8880292.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="ACHW4Z_000520"
     CDS             complement(40421..41815)
                     /locus_tag="ACHW4Z_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8880293.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="ACHW4Z_000521"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="ACHW4Z_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR8880294.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="ACHW4Z_000522"
     CDS             complement(42158..43444)
                     /locus_tag="ACHW4Z_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8880295.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="ACHW4Z_000523"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="ACHW4Z_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="ACHW4Z_000524"
     CDS             complement(44451..45221)
                     /locus_tag="ACHW4Z_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR8880296.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="ACHW4Z_000525"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="ACHW4Z_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR8880297.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="ACHW4Z_000526"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="ACHW4Z_000526"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR8880298.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="ACHW4Z_000527"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="ACHW4Z_000527"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR8880299.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="ACHW4Z_000528"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="ACHW4Z_000528"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR8880300.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="ACHW4Z_000529"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="ACHW4Z_000529"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR8880301.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="ACHW4Z_000530"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="ACHW4Z_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR8880302.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="ACHW4Z_000531"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="ACHW4Z_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR8880303.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="ACHW4Z_000532"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="ACHW4Z_000532"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR8880304.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADVTEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="ACHW4Z_000533"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="ACHW4Z_000533"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR8880305.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="ACHW4Z_000534"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="ACHW4Z_000534"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR8880306.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="ACHW4Z_000535"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="ACHW4Z_000535"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR8880307.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="ACHW4Z_000536"
                     /gene_synonym="lytB"
     CDS             complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="ACHW4Z_000536"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR8880308.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="ACHW4Z_000537"
     CDS             complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="ACHW4Z_000537"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8880309.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="ACHW4Z_000538"
     CDS             complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="ACHW4Z_000538"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR8880310.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="ACHW4Z_000539"
     CDS             complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="ACHW4Z_000539"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR8880311.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="ACHW4Z_000540"
     CDS             complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="ACHW4Z_000540"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR8880312.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66109..66327)
                     /locus_tag="ACHW4Z_000541"
     CDS             complement(66109..66327)
                     /locus_tag="ACHW4Z_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR8880313.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66430..66693
                     /gene="rpsT"
                     /locus_tag="ACHW4Z_000542"
     CDS             66430..66693
                     /gene="rpsT"
                     /locus_tag="ACHW4Z_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR8880314.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16260 a        18246 c        17252 g        15264 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcttga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcac
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttttat gaagaaaaca tgccggtaac aagaccggca ttttcgcata
    60301 acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct ctttcggcac
    60361 ttcgaaaaca atattttctt cacggccttc cagcggaatg gcttcaccac cacccaactg
    60421 ctgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc ccgcagtcac
    60481 gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat cgtcaatcaa
    60541 aaacgcgtgt ttgcccatac gctgggccag ctccgccaga cggttggagt tggaggagtt
    60601 tttcgaaccg accaccaaca caacttccgc ctgttctgcc agggcgcgta ccgcttcctg
    60661 acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt tcgggaagcg
    60721 tttacgcagc gcgtcgatca catcagacgt gtcatccacc gacagcgtgg tttgggtcat
    60781 aaaggagagc ttctcttcgt ttttgaccgt cagtttccac acatcgtctg gcgattcgac
    60841 tagatacatt cccccttcgg ggttgctgta ctgccccatc gtcccttcca cttccgggtg
    60901 cccggcgtgg ccgatgagaa tagattcttc gccacggcga ctggcgcggg caacttccat
    60961 atgcactttg gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc ggcttttcgc
    61021 ttcgttacga accgcctgag aaacaccgtg tgcggagaaa atcaggatcg cgccgtccgg
    61081 cacttcgcta atctgctcaa taaagatagc cccacgctcg cgcaagctat cgaccacgta
    61141 gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg ccagcgcgtt
    61201 ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt tggccaacag
    61261 gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata tcaaaatgaa
    61321 cggtctgccc ggccagcgga tggttgaaat caacggtaat ggagtcgccg ttaatttcgc
    61381 ggatcacacc aggcatctca ctgccatcca ttgcggtaaa aagcatgatt gcgccaattt
    61441 ctggctcgcc tgcatccata aattcacggc gggagaagta ctgaatcagg tccggtgacg
    61501 gcacgccaaa cgccgcatct ggctccagcg agaaggtggt tttgtcgccc actttcagcc
    61561 ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg aacagcgccg
    61621 gtttaccgtt gttgcgagta gactcggcgg tggtgccatc gtcgagtttt agcgtgaagt
    61681 gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc tcttattttt
    61741 gaggattttt gccggatgcg gcctacgatt tcgtagcaat ctgtaggccg catccggcag
    61801 gatttattat tgttttttcg ctttggaagg taaaaaccct tccagcacga tcagtgccgc
    61861 accgacacaa atggcagtat cggcaaggtt gaaggtggcg aagtgccagt cgccgacgta
    61921 gaagtcgatc atatcgacaa cgaagccgtg ccacaggcgg tcgaacaggt tgcccagcgc
    61981 gccgccaata atcagcgcgt aagcgatatt gtttagcttc tgcgtggcct tcgagcgata
    62041 catcatcact gccaggagca cgctaatacc aatcgcaata ccggcaaaga accaacgctg
    62101 ccagccgccg ctatcggcaa ggaaactaaa cgccgcgcca tagttacgcg cataatgcag
    62161 attaagcgac gggaacagcg ggaccgtatc ccccagagca aagttctgga ggatcaggta
    62221 tttgctgccc agatcgataa tcagcacgac taccaccagc cacagccagc gtagccctgt
    62281 tgaacagatc gattgactca tcaggcaaac ttacgttttt caccgtcacc ggcgacgttg
    62341 ctgacacagc ggccgcagat ttctgcgtgt tccgccacct tgccgacatc ctgggtgtag
    62401 tgccagcagc gtgggcactt ctcaccttcg gctttactca acgcgacttt cagccctttg
    62461 agtacttcgc tctgctgagc atcagcaggt gcgtcgttat agtctgcaac ggtagcgccg
    62521 gaggtcaaca ggacaaatcg taattcatcg cccagcgcgg tcagtttcgc cgccagttcc
    62581 ggttctgcat acaaggttac tgccgcttcc agcgagccac ccactttctt gtcggcacgc
    62641 gcttgctcaa tgactttgtt cacttcgcca cgcactttca acagctcgtc ccagaacgca
    62701 tcgttcattg cttcactgtc tgccagacca aacaggcctt cgtaccactc accggtgaag
    62761 acgtattttt cacgttcgcc cggcagatag ccccacactt catcagcggt gaaggagagg
    62821 attggtgcca tccagcgcac cagcgcttca gcgatgtgat acagcgcagt ctggcagcta
    62881 cgacgcgcca cgctatccgc tttggcggtg tactgacggt ctttgatgat gtcgaggtag
    62941 aaggaaccca tctcaacgga gcagaagcgc atcagacgct gtaccacttc gtggaaatcg
    63001 tatgcttcgt acgccttgag gatgtcttcc tgtgccgctt tcgcacaacc tacggcccag
    63061 cgatccagta ccaccatctc ttccggtttc accatatctt ttgctggatc aaaaccattc
    63121 aggtttgcca gcaggaagcg cgcggtgtta cggatacgac gatagctatc ggcagcacgt
    63181 ttcaggatct cgtcagaaac ggccatttca ccggtgtagt cggttgatgc cacccacaga
    63241 cgcagaatat ccgcgcccag tttgttcatc acatcctgcg gcgaaacggt gttgccgatg
    63301 gatttagaca tcttgcgacc ctgaccatcc acggtaaagc cgtgggtcag tacctgacga
    63361 tacggcgctt tgcctttcat cgcggtggag atcattaggg aagacatgaa ccaaccacgg
    63421 tgctggtcag aaccttccag atacatgtcc gctgcgtgac cggcaaattc cggacgcacg
    63481 tcaacaacag aagagtgggt agatccggag tcaaaccata cgtcgagagt atccggcact
    63541 ttcacatact ggtcagcttc gtcgccgagg atctctttcg catcgagatc ccaccacgcc
    63601 tggatgccgt caacttcaac gcgtttagcc acttcttcca tcagttcgag ggtacgcgga
    63661 tgcagctctt ccgtgtcttt gtgcacgaac agtgacatcg gtacgcccca ggtgcgctga
    63721 cgggagatac accagtcagg acggttagca accatcgact cgatacgcgc ctggccccag
    63781 tccgggagcc actgcacgcc tttgatctct ttcagtgact gcgcacgcag acctttctga
    63841 tccatgctga cgaaccactg cggtgtcgcg cggaagatga tcggcgtttt gtgacgccag
    63901 cagcacggat agctgtgctg cattttctca acgtgcagca gcgcgccttt ttcctgcagc
    63961 agcgcaacga cgatgtcgtt cgctttgaag acgttcacgc catccagcgt cggataagtg
    64021 cccggcagat aagtgccgtc cgggccaacc gggttagcgg tttccaggcc gtatttctga
    64081 ccgatcacat agtcgtccgg gccgtggcca ggcgcggtgt gaacggcacc ggtaccggca
    64141 tccagggtaa cgtgatcgcc gaggattgcc ggaacgtcga agcccataaa cggatgggta
    64201 aagcgcagca gctcaagctc tgcacctttt accgtgccga gaatggtgta atcggtcacg
    64261 ccgatacgct gcattacgct ttcaaccaga tctttcgcca gaatcacggc ctgaccgtcg
    64321 atctgcacca gcgcatagtc gaaatcaggt gcaatagaga ttgcgcggtt cgcaggcaga
    64381 gtccacggcg tagtggtcca gatcaccagc gagattgggc cgttaacgtt gcttacgcca
    64441 aatttcgctt tcagcgcatc ctgatcgacc gcctggaaag cgacgtcaat ggacggagaa
    64501 gttttgtcgt aatactcaac ttccgcttct gccagtgcag aacggcagtc tacgcaccag
    64561 tgcaccggct tcgcgccttt gtgcaggtga ccattgccga tgattttgcc cagcgcgcgg
    64621 atgatgttgg cttcagtttt gaagtccata gtcagatacg ggtgcgacca gtcgcccagc
    64681 acgcccagac ggataaagtc tttgcgttga ccgtcaacct gggtcgccgc gtattcgcgg
    64741 cacttggcgc ggaactcggc ggcggtgaat ttctcacccg gcttaccgta ttcttgctct
    64801 actttcagct cgatcggcag accgtggcag tcccagccag gcacatacgg cgagtcataa
    64861 ccggaaagcc ctttggactt cacgataatg tctttcagaa tcttgttaac cgagtgacca
    64921 atatgaatgc tgccattcgc ataaggaggg ccatcatgca gaatgaaggt ttttttgcct
    64981 tttttagccg cacggatgat gccgtacaga tcatcatcag tccaacgcgc cagcattccc
    65041 ggttcgcgct tggcgagatc gccacgcatc gggaaccctg tttccggcaa attcagggtt
    65101 gatttatagt cactcatcag attctcggtt ccgtgtttcg gtttgattac ataacaggct
    65161 taagccggtt ttgttagccc aaaaaattcg cgggcggtta attcatcacg cgcaatctgc
    65221 gctttcagtt cgtccagcga cgcaaatcgc tgctcattgc gtattttttt acgcagcact
    65281 acttgtatat ggcgaccgta aaggtccatt gcaacatcta acaaatgcac ttccagctgc
    65341 tggcgaatac cggcaaccgt tgggcgtgtt ccgatgtttg ccacgccggg taacggcttt
    65401 tcaccgaggc ccagcacttc taccgcataa acccctttca ccggggaaac ctgacggcgt
    65461 agcggtacat tcgccgtcgg gaaacctata gtgcgcccta attcatcacc gtggactaca
    65521 cgcccggaga tggcaaacgg gtgccccagt aaactttctg ccagagccag attgtcatca
    65581 gccagcgcct gacgcacggc ggtgctgctg atacgcacac caccttcgca aaaagtttgc
    65641 gtactggtga tatcgaagcc gtattccatg ccagctttct gtaataacaa gaaatcgcct
    65701 tcacgaccag cgccaaagcg gaaatcatca cctacggcaa gaaattttac gcgcaaatgc
    65761 ttcaccaaaa gatcgctgat gaaattttgc gcggttaacg ccgcaaaacg cctgtcgaaa
    65821 cgcacgcaca gcacgtaatc aacgccacac tctgcaaggt aacgcagttt ttcccgcagc
    65881 cgggtcagtc ttgccggggc tttatcggta gcaaacagtt ccagtggttg aggttcaaaa
    65941 agcatcacca tcaccggtaa gttgcgcttg cgcccttctt cctgcaagcc ctgtaacagc
    66001 gcgcgatgac cgcgatgcac gccgtcgaaa ttaccaatag tcagcacaca cccttcttgc
    66061 ggggcctggc tgagattatg tatgccgcgt atcagcttca tgtctggctc aaaacagtga
    66121 aaatcgtccg agtatacctt gtacagcggt caaggttaac cggcgattga gtaccgaaat
    66181 cagaaagaag tggcttttca ttgccatgga gcaaatcacg ggaagaaact gaccgcctgc
    66241 tgcaattttt atcgcggaaa agctgtattc acaccccgca agctggtaga atcctgcgcc
    66301 atcactacgt aacgagtgcc ggcacattaa cggcgcttat ttgcacaaat ccattgacaa
    66361 aagaaggcta aaagggcata ttcctcggcc tttgaattgt ccatatagaa cacatttggg
    66421 agttggacct tggctaatat caaatcagct aagaagcgcg ccattcagtc tgaaaaggct
    66481 cgtaagcaca acgcaagccg tcgctctatg atgcgtactt tcatcaagaa agtatacgca
    66541 gctatcgaag ctggcgacaa agctgctgca cagaaagcat ttaacgaaat gcaaccgatc
    66601 gtggaccgtc aggctgctaa aggtctgatc cacaaaaaca aagctgcacg tcataaggct
    66661 aacctgactg cacagatcaa caaactggct taatcgccaa tttgctgaag ctttgtgaaa
    66721 aagcccgcgc aagcgggttt ttttatgcct gctgcttttg tgatgcgtga gagaacacac
    66781 tgccggttgc cagatagtca aaagagacat gccgttgtac tatctgaact cttcgtggct
    66841 taagaatcgt agaaccatcg gctgatacat tctgaacaag aatatctact caatctccag
    66901 actcccccac aaagaatatg gatattgtga tacacattga ggtattaaac ttaagaccac
    66961 tctatttttg aattaaattc ctatatactt taaccattca taaatatctc ctcattagac
    67021 ct
//
LOCUS       DAZSNG010000008        65760 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZSNG010000008 DAZSNG010000000
VERSION     DAZSNG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.008"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="ACHW4Z_000543"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="ACHW4Z_000543"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR8880315.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACHW4Z_000544"
     CDS             complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACHW4Z_000544"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR8880316.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACHW4Z_000545"
     CDS             complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACHW4Z_000545"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR8880317.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACHW4Z_000546"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACHW4Z_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR8880318.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACHW4Z_000547"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACHW4Z_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR8880319.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACHW4Z_000548"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACHW4Z_000548"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR8880320.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="ACHW4Z_000549"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="ACHW4Z_000549"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR8880321.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="ACHW4Z_000550"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="ACHW4Z_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR8880322.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="ACHW4Z_000551"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="ACHW4Z_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR8880323.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="ACHW4Z_000552"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="ACHW4Z_000552"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR8880324.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="ACHW4Z_000553"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="ACHW4Z_000553"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR8880325.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="ACHW4Z_000554"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="ACHW4Z_000554"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR8880326.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="ACHW4Z_000555"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="ACHW4Z_000555"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR8880327.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17800..17847
                     /gene="yadX"
                     /locus_tag="ACHW4Z_000556"
     CDS             17800..17847
                     /gene="yadX"
                     /locus_tag="ACHW4Z_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR8880328.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="ACHW4Z_000557"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="ACHW4Z_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR8880329.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /gene="yadW"
                     /locus_tag="ACHW4Z_000558"
     CDS             19304..19369
                     /gene="yadW"
                     /locus_tag="ACHW4Z_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR8880330.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="ACHW4Z_000559"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="ACHW4Z_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR8880331.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="ACHW4Z_000560"
     CDS             complement(19753..20376)
                     /locus_tag="ACHW4Z_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR8880332.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="ACHW4Z_000561"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="ACHW4Z_000561"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR8880333.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="ACHW4Z_000562"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="ACHW4Z_000562"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR8880334.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="ACHW4Z_000563"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="ACHW4Z_000563"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR8880335.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="ACHW4Z_000564"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="ACHW4Z_000564"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR8880336.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="ACHW4Z_000565"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="ACHW4Z_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="ACHW4Z_000566"
     CDS             complement(26460..26846)
                     /locus_tag="ACHW4Z_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR8880337.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="ACHW4Z_000567"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="ACHW4Z_000567"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR8880338.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="ACHW4Z_000568"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="ACHW4Z_000568"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR8880339.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="ACHW4Z_000569"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="ACHW4Z_000569"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR8880340.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="ACHW4Z_000570"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="ACHW4Z_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR8880341.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="ACHW4Z_000571"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="ACHW4Z_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR8880342.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="ACHW4Z_000572"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="ACHW4Z_000572"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR8880343.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="ACHW4Z_000573"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="ACHW4Z_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR8880344.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="ACHW4Z_000574"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="ACHW4Z_000574"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR8880345.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="ACHW4Z_000575"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="ACHW4Z_000575"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR8880346.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="ACHW4Z_000576"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="ACHW4Z_000576"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR8880347.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="ACHW4Z_000577"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="ACHW4Z_000577"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR8880348.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="ACHW4Z_000578"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="ACHW4Z_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR8880349.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="ACHW4Z_000579"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="ACHW4Z_000579"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR8880350.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="ACHW4Z_000580"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="ACHW4Z_000580"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR8880351.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="ACHW4Z_000581"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="ACHW4Z_000581"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR8880352.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="ACHW4Z_000582"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="ACHW4Z_000582"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR8880353.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="ACHW4Z_000583"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="ACHW4Z_000583"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR8880354.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="ACHW4Z_000584"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="ACHW4Z_000584"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR8880355.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="ACHW4Z_000585"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="ACHW4Z_000585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR8880356.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="ACHW4Z_000586"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="ACHW4Z_000586"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR8880357.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="ACHW4Z_000587"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="ACHW4Z_000587"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR8880358.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="ACHW4Z_000588"
     CDS             53914..54303
                     /locus_tag="ACHW4Z_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8880359.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="ACHW4Z_000589"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="ACHW4Z_000589"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR8880360.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="ACHW4Z_000590"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="ACHW4Z_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR8880361.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="ACHW4Z_000591"
     CDS             complement(55962..56162)
                     /locus_tag="ACHW4Z_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR8880362.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="ACHW4Z_000592"
     CDS             56328..56873
                     /locus_tag="ACHW4Z_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR8880363.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="ACHW4Z_000593"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="ACHW4Z_000593"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR8880364.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSRELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="ACHW4Z_000594"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="ACHW4Z_000594"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR8880365.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="ACHW4Z_000595"
     CDS             complement(58171..58995)
                     /locus_tag="ACHW4Z_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR8880366.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="ACHW4Z_000596"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="ACHW4Z_000596"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR8880367.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="ACHW4Z_000597"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="ACHW4Z_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR8880368.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="ACHW4Z_000598"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="ACHW4Z_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR8880369.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="ACHW4Z_000599"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="ACHW4Z_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR8880370.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /locus_tag="ACHW4Z_000600"
     CDS             complement(62958..63611)
                     /locus_tag="ACHW4Z_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR8880371.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="ACHW4Z_000601"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="ACHW4Z_000601"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR8880372.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="ACHW4Z_000602"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="ACHW4Z_000602"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR8880373.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15557 a        16289 c        18109 g        15805 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcggg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
//
LOCUS       DAZSNG010000009        62484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZSNG010000009 DAZSNG010000000
VERSION     DAZSNG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.009"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHW4Z_000603"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHW4Z_000603"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR8880374.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHW4Z_000604"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHW4Z_000604"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR8880375.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHW4Z_000605"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHW4Z_000605"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR8880376.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHW4Z_000606"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHW4Z_000606"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR8880377.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHW4Z_000607"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHW4Z_000607"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR8880378.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHW4Z_000608"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHW4Z_000608"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8880379.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHW4Z_000609"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHW4Z_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR8880380.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHW4Z_000610"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHW4Z_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8880381.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHW4Z_000611"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHW4Z_000611"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR8880382.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHW4Z_000612"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHW4Z_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR8880383.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHW4Z_000613"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHW4Z_000613"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR8880384.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACHW4Z_000614"
     CDS             complement(15665..16702)
                     /locus_tag="ACHW4Z_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8880385.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHW4Z_000615"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHW4Z_000615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8880386.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHW4Z_000616"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHW4Z_000616"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR8880387.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACHW4Z_000617"
     CDS             19444..19884
                     /locus_tag="ACHW4Z_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR8880388.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHW4Z_000618"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHW4Z_000618"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8880389.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACHW4Z_000619"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACHW4Z_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHW4Z_000620"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHW4Z_000620"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR8880390.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHW4Z_000621"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHW4Z_000621"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR8880391.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHW4Z_000622"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHW4Z_000622"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR8880392.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHW4Z_000623"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHW4Z_000623"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR8880393.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHW4Z_000624"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHW4Z_000624"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR8880394.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHW4Z_000625"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHW4Z_000625"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR8880395.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHW4Z_000626"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHW4Z_000626"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR8880396.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHW4Z_000627"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHW4Z_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR8880397.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="ACHW4Z_000628"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="ACHW4Z_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR8880398.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACHW4Z_000629"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACHW4Z_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR8880399.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACHW4Z_000630"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACHW4Z_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR8880400.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACHW4Z_000631"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACHW4Z_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR8880401.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACHW4Z_000632"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACHW4Z_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR8880402.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36679)
                     /gene="ftsY"
                     /locus_tag="ACHW4Z_000633"
     CDS             complement(35180..36679)
                     /gene="ftsY"
                     /locus_tag="ACHW4Z_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR8880403.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEAAKEEITDEELEAQA
                     LAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFIS
                     LFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEE
                     MGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTF
                     RAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNK
                     SHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKL
                     DGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36829..37425
                     /gene="rsmD"
                     /locus_tag="ACHW4Z_000634"
     CDS             36829..37425
                     /gene="rsmD"
                     /locus_tag="ACHW4Z_000634"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR8880404.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37415..37684
                     /locus_tag="ACHW4Z_000635"
     CDS             37415..37684
                     /locus_tag="ACHW4Z_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR8880405.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37687..38046)
                     /locus_tag="ACHW4Z_000636"
     CDS             complement(37687..38046)
                     /locus_tag="ACHW4Z_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR8880406.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38187..38813
                     /locus_tag="ACHW4Z_000637"
     CDS             38187..38813
                     /locus_tag="ACHW4Z_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR8880407.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38887..41085
                     /gene="zntA"
                     /locus_tag="ACHW4Z_000638"
     CDS             38887..41085
                     /gene="zntA"
                     /locus_tag="ACHW4Z_000638"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR8880408.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41187..41432)
                     /gene="tusA"
                     /locus_tag="ACHW4Z_000639"
     CDS             complement(41187..41432)
                     /gene="tusA"
                     /locus_tag="ACHW4Z_000639"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR8880409.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41653..42318
                     /locus_tag="ACHW4Z_000640"
     CDS             41653..42318
                     /locus_tag="ACHW4Z_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR8880410.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42391..42948
                     /gene="dcrB"
                     /locus_tag="ACHW4Z_000641"
     CDS             42391..42948
                     /gene="dcrB"
                     /locus_tag="ACHW4Z_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR8880411.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42952..44169)
                     /locus_tag="ACHW4Z_000642"
     CDS             complement(42952..44169)
                     /locus_tag="ACHW4Z_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8880412.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44303..45352
                     /locus_tag="ACHW4Z_000643"
     CDS             44303..45352
                     /locus_tag="ACHW4Z_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8880413.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45407..45994
                     /gene="acpT"
                     /locus_tag="ACHW4Z_000644"
     CDS             45407..45994
                     /gene="acpT"
                     /locus_tag="ACHW4Z_000644"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR8880414.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46105..47679
                     /gene="nikA"
                     /locus_tag="ACHW4Z_000645"
     CDS             46105..47679
                     /gene="nikA"
                     /locus_tag="ACHW4Z_000645"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8880415.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47679..48623
                     /gene="nikB"
                     /locus_tag="ACHW4Z_000646"
     CDS             47679..48623
                     /gene="nikB"
                     /locus_tag="ACHW4Z_000646"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR8880416.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48620..49453
                     /gene="nikC"
                     /locus_tag="ACHW4Z_000647"
     CDS             48620..49453
                     /gene="nikC"
                     /locus_tag="ACHW4Z_000647"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR8880417.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49453..50217
                     /gene="nikD"
                     /locus_tag="ACHW4Z_000648"
     CDS             49453..50217
                     /gene="nikD"
                     /locus_tag="ACHW4Z_000648"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR8880418.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50214..51020
                     /gene="nikE"
                     /locus_tag="ACHW4Z_000649"
     CDS             50214..51020
                     /gene="nikE"
                     /locus_tag="ACHW4Z_000649"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR8880419.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51026..51427
                     /gene="nikR"
                     /locus_tag="ACHW4Z_000650"
     CDS             51026..51427
                     /gene="nikR"
                     /locus_tag="ACHW4Z_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR8880420.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51547..51906)
                     /locus_tag="ACHW4Z_000651"
     CDS             complement(51547..51906)
                     /locus_tag="ACHW4Z_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR8880421.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51903..52178)
                     /locus_tag="ACHW4Z_000652"
     CDS             complement(51903..52178)
                     /locus_tag="ACHW4Z_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR8880422.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52237..53361)
                     /locus_tag="ACHW4Z_000653"
     CDS             complement(52237..53361)
                     /locus_tag="ACHW4Z_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8880423.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53361..56096)
                     /gene="rbbA"
                     /locus_tag="ACHW4Z_000654"
     CDS             complement(53361..56096)
                     /gene="rbbA"
                     /locus_tag="ACHW4Z_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR8880424.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56093..57160)
                     /locus_tag="ACHW4Z_000655"
     CDS             complement(56093..57160)
                     /locus_tag="ACHW4Z_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8880425.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57223..57336)
                     /gene="yhiY"
                     /locus_tag="ACHW4Z_000656"
     CDS             complement(57223..57336)
                     /gene="yhiY"
                     /locus_tag="ACHW4Z_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR8880426.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57526..59148)
                     /locus_tag="ACHW4Z_000657"
     CDS             complement(57526..59148)
                     /locus_tag="ACHW4Z_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8880427.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59410..60999)
                     /locus_tag="ACHW4Z_000658"
     CDS             complement(59410..60999)
                     /locus_tag="ACHW4Z_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8880428.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61416..62414
                     /locus_tag="ACHW4Z_000659"
                     /pseudo
     CDS             61416..62414
                     /locus_tag="ACHW4Z_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT        14905 a        16991 c        15626 g        14962 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ttggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacagact cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttcttctgcc gcttcgacaa tctctacggt ttccgcttca gcctgccact
    36301 cttctgacga aacctcttcg gcgttgacgt cttccggcaa cggcagctct tcacgttcga
    36361 tagccactgg ctccggcgtt tcttctacga ccggttccgg ctgtgcaaca acttccgctt
    36421 caggctgcgc tttttcactt tcaacaacct gttcagtgac ttccacaacg tcggcagcaa
    36481 aagtttccgc ctcggcttca gtatgcgcct gcggctgctc ttcaacggct tgttcagaag
    36541 ccttcgcagg ctcttgcgcc tgaacgattt cttctacaac cggttgttca ttctgaactt
    36601 ctgtctcttt ttccggggtc tgctcttttt gaccaaagcc cagccaggaa aaaaagccac
    36661 gtcttttttc tttcgccatt tgcgactaca ctcctcgctg ttgcttcatg gcacagcgtt
    36721 aacgctatgt acatagcagc taaaaaaatg atgaaatagt ctatcactta acttaattca
    36781 catcaccgcc tgcaagtatg tgttatctgg cggattgagc aatttatcat gaaaaaaccg
    36841 aatcattccg gcagcggcca aatccgcatt attggcgggc agtggcgagg ccgtaaactc
    36901 ccggttcctg atagcccagg tctgcgcccc accaccgacc gcgtacgcga aacgttgttt
    36961 aactggctgg ctccggtcat tgttgacgcc caatgtctgg attgcttcgc cgggagcggc
    37021 gcgctggggc tggaagcgtt atcgcgctac gctgcggggg caacgttgat tgagatggat
    37081 cgcgtggttt ctcagcagtt aattaagaat ctggcgacac taaaagcagg caatgcacgc
    37141 gtggtgaaca gcaacgcaat gtcattcctg gcgcaaaaag gcacaccgca taatatcgtg
    37201 tttgtcgatc caccgttccg ccgtggcttg ttagaagaga cgataaattt actggaagat
    37261 aacggctggc tggctgacga agccctgatt tatgtcgaaa gcgaagtcga aaacggtctg
    37321 cccactgttc cggcaaactg gtcattacat cgggaaaaag tggcgggtca ggtggcttat
    37381 cggctgtatc aacacgaagc acaaggagaa agtgatgctg attaatattg gtcgtttgtt
    37441 aatgctctgc gtttggggat ttttaatcct caatctggtg catcccttcc cgcgcccgct
    37501 aaatattttc gttaacgtgg cgctgatttt taccgtgctg atgcatggta tgcagctggc
    37561 gctattgaaa tccactttac cgaaagatgg cccgcagatg accaccgccg aaaaggtgcg
    37621 gattttcctt ttcggcgtgt ttgaactgct ggtctggcag aagaaattta aagttaaaaa
    37681 ataaccttat tgttcgccaa caaagctaac aaagcgcgtt cctttatagc tcagcgtacc
    37741 tttatcgccc actgtcaggg cgtggtactg ctgggcgtcg aggcgaaacg tctgctccat
    37801 tcctccgctt tgcggtttga agcttgcctc atagcgcata ctggtgcctg ccggagtcac
    37861 ttcctgctgg cgcgaacggc gatcgttaat cggtttttcc cgcttgttgc tcaccaccac
    37921 cagcttttgc tggagcggag ccatatcatt atcagctttt tcccgtcgct gttgcataaa
    37981 acgaaacgat gcggcgacga caattaagcc aatgataaca ataaagaaaa gaggtggttt
    38041 actcatcttt atccctcatc ggaaaatgcg gaattaagca taccctgcca gttatggtgt
    38101 tgtcatccgt ccaccctcgc cactaaactg gaagcaagac cgtaggcatt ctgcttacga
    38161 aaaaataacg aattcaagga actaagatgc tttggtcgtt tatcgctgtc tgtctttccg
    38221 catggctatc tgtggatgca tcgtatcgtg ggccaacctg gcaacgctgg gtgtttaaac
    38281 cgttaaccct tcttctcctg ctgttactgg cctggcaagc accgatgttc gacgccatta
    38341 gctatctggt gctggcggga ctgtgcgcct cactgctggg cgatgcgcta accctgttgc
    38401 cacgtcaacg tctgatgtac gccatcggcg cgtttttcct ctcgcacctg ctgtacacca
    38461 tctatttcgc cagtcagatg acgctctctt tcttctggcc tctgccactg gtgttgctgg
    38521 ttctgggtgc gctgctactg gcgattatct ggacgcggct ggaagagtac cgttggccta
    38581 tctgcacgtt tatcggcatg acgctggtga tggtgtggct ggcaggtgaa ctgtggttct
    38641 tccgtccgac cgctccggcg ctctctgcgt ttgttggcgc ttcgttgctg tttatcagta
    38701 actttgtctg gctggggagc cactatcgcc gacgcttccg tgcggataac gcgattgctg
    38761 cggcctgcta ctttgccggt cacttcctca tcgtccgctc actgtatctc tgataaaact
    38821 tgactctgga gtcgactcca gagtgtatcc ttcggttaat gagaaaaaac ttaaccggag
    38881 gatgccatgt cgacacctga caatcacggc aagaaagccc ctcaatttgc tgcgttcaaa
    38941 ccgctaacca cggtacagaa cgccaacgac tgttgctgcg acggcgcatg ctccagctcg
    39001 ccaacgctct ctgaaaacgt ctccggcacc cgctatagct ggaaagtcag cggcatggac
    39061 tgcgccgcct gtgcgcgcaa ggtagaaaat gccgtgcgcc agcttgcagg cgtgaatcag
    39121 gtgcaggtgt tgttcgccac cgaaaaactg gtggttgatg ccgacaatga catccgtgcc
    39181 caggttgaat ctgcggtgca aaaagcgggc tattccctgc gcgatgagca ggccgccgac
    39241 gagccacaag catcgcgcct gaaagagaat ctgccgctga ttacgcttat cgtgatgatg
    39301 gcaatcagct ggggtctgga gcagttcaat catccgttcg ggcaactggc gtttatcgcg
    39361 accacgctag ttgggctgta cccgattgct cgtcaggcat tacggctgat caaatccggc
    39421 agctacttcg ccattgaaac cttaatgagc gtagccgcta ttggtgcact gtttattggc
    39481 gcaacggctg aagctgcgat ggtgttgctg ctgtttttga ttggtgaacg actggaaggc
    39541 tgggccgcca gccgcgcgcg tcagggcgtt agcgcgttaa tggcgctgaa accagaaacc
    39601 gccacgcgcc tgcgtaacgg tgagcgggaa gaggtggcga ttaacagcct gcggcctggc
    39661 gatgtgattg aagtcgccgc aggtgggcgt ttgcctgccg acggtaaact gctctcaccg
    39721 tttgccagtt ttgatgaaag cgccctaacc ggagaatcca ttccggtgga gcgcgcgacg
    39781 ggcgataaag ttcctgcagg agccaccagc gtagaccgtc tggtaacgct ggaagtgctg
    39841 tcagaaccgg gtgccagcgc cattgaccgg attctgaaac tgatcgaaga agccgaagag
    39901 cgccgcgccc ccattgagcg gtttatcgac cgtttcagcc gtatttatac gccagcgatt
    39961 atggccgtcg ccctgctggt aacgctagtg ccgccgctgc tgtttgccgc cagctggcag
    40021 gagtggattt ataaaggtct gacgctgctg ctgattggtt gcccgtgtgc gttagttatc
    40081 tcaacgcctg cggcgataac ctccgggctg gcagcagcag cgcgtcgtgg ggcgttaatt
    40141 aaaggcggtg cggcgctgga acaactaggt cgtgttaccc aagtggcgtt tgataaaacc
    40201 ggtacgctga ccgtcggtaa accgcgcgtt accgcgattc atccggcaac gggtattagt
    40261 gaatctgaac tgctgacact ggcggcggcg gtcgagcaag gcgcgacgca tccactggcg
    40321 caagccatcg tacgcgaagc acaggttgct gaactcgcca ttcccaccgc cgaatcacag
    40381 cgggcactgg tcgggtctgg cattgaagcg caggttaatg gtgagcgcgt gttgatatgc
    40441 gctgccggga aacatcccgc tgatgcattt gctggtttga ttaatgaact ggaaagcgcc
    40501 gggcaaacgg ttgtgctggt agtacgtaat gatgacgtgc tgggtatcat tgcattgcag
    40561 gataccctgc gcgccgacgc tacaactgcc atcagtgaac tgaacgcgct gggggttaaa
    40621 ggagtgatcc tcaccggcga taatccacgc gcagcggcgg caattgccgg ggagctgggg
    40681 ctggagttta aagcgggcct attgccggaa gataaagtca aagcggtgac caagctgaat
    40741 caacatgcgc ccctggcgat ggtcggtgat ggtattaacg acgcgccagc gatgaaagct
    40801 gccgccatcg ggattgctat gggtagcggc acagacgtgg cgctggaaac cgccgacgca
    40861 gcattaaccc ataaccacct gcgcggcctg gtgcaaatga ttgaactggc acgcgccact
    40921 cacgccaata tccgccagaa catcaccatt gcgctgggac tgaaagggat cttcctcgtc
    40981 accacgcttt tagggatgac cgggttgtgg ctggcagtgc tggcagatac gggtgcgacg
    41041 gtgctggtga cggcgaatgc gttaaggttg ttgcgcagga gataaggcaa accgatcgca
    41101 acattgagcg cgatcggtcc cctcgcccct ctggggagag ggttaggggg tgaggaaaag
    41161 gcggcatcga agccaatcag cccctatcaa ccgcctttac gaatcaaata acgataaggc
    41221 agtccatccg tctctttcgc aaccagttcg tgttccataa aggtacaaaa cccaggaata
    41281 tcgcgggtag tggccggatc gtcggcgata atcagcaacg tttcgccagg ctgcatattg
    41341 cgcacggttt tgcgcaccat catcaccggt tccgggcagc gcaggccaag cgcgtcgagt
    41401 gtgtggtcag ggctggaaaa gagatcggtc attttcttct catcacttaa aaaaacggcg
    41461 ctagtttacg ccctgtgagt ccgtaagcaa accaggttaa cgattgcgtg aaaattaacc
    41521 attgcattgt caacgtaaag cagtatcatg cggcggctcg aaaaaagggt aagcacgtta
    41581 ttatgttaag gtaacagacg tgtcgtacgt attgggttcc ctcaccccaa tggttaatca
    41641 aaaaggtaca atatgaacgt tttctcgcaa actcaacgct ataaggcgtt gttctggtta
    41701 tcgttatttc atcttctggt gatcacctcc agtaactatc tggttcagct tcccgtctcc
    41761 atttttggtt tccataccac ctggggcgcg tttagctttc cgtttatttt tcttgctacc
    41821 gacctgaccg tgcgtatttt tggcgcaccg ctggcccgac gcattatctt cgcggtaatg
    41881 atccctgcgt tattaatctc ctacgtcatc tcgtcgctat tctatatggg ttcctggcag
    41941 ggattcggcg cactcgccca cttcaacctg tttgtcgccc gtatcgccac cgccagtttc
    42001 atggcctacg cgctggggca aatcctcgac gtgcacgttt ttaaccgcct gcgtcagagt
    42061 cgccgctggt ggctggcacc gacagcgtcc acactgttcg gtaacgtcag cgacacgctg
    42121 gcctttttct tcattgcctt ctggcgtagc ccggatgcct ttatggctga acactggatg
    42181 gaaatcgcgc tggtcgatta ctgtttcaaa gtgttaatca gtatcgtttt cttcctgcca
    42241 atgtatggcg tattactcaa tatgctgttg aaaagactgg cagataaatc cgaaatcaac
    42301 gctttgcagg cgagttaaag gttcgttatc agagttgtga taagatggat gaatgagccg
    42361 ttatggccgt ttatcgaaag gaagaagtca atgcgcaatc tggttaaata tgtcggaatt
    42421 ggcctgctgg ttatggggct tgcggcctgt gatgataaag acactaacgc tacggcgcag
    42481 ggttcggttg cggaaagtaa cgctaccggg aatcccgtca acctgcttga tggcaagtta
    42541 agtttctcgc tgccagcgga tatgaccgac cagagcggta agctgggaac gcaggccaat
    42601 aacatgcatg tctggtccga cgccaccggg cagaaagcag tcatcgtcat catgggcgat
    42661 gatccgaaag aagatctggc ggtgttggcg aagcgtctgg aagatcagca acgtagccgc
    42721 gatccgcagc tgcaagtggt aaccaataaa gccattgagc tgaaaggtca caaaatgcag
    42781 cagttagaca gtattatctc cgcgaaaggc cagacggcgt actcttccgt tattctgggt
    42841 aacgtgggta atcaactgct gaccatgcaa attacgctgc cagctgacga tcagcaaaaa
    42901 gcgcaaacca ccgcagaaaa catcattaat acgctggtta ttcagtaagt tttaagatga
    42961 tgaggcggcc tcagggacgt gttccggagg ccgttttttt aatcgccacg tcagtaataa
    43021 cgcgattgcg accagtcccg ccgccgccag ataaatcacc ggtacgcccg cccagctcat
    43081 caccagccca gccagtggtc cagtcacgcc aagcgataaa tccataaata cggtgtaagt
    43141 tgccagcgcc gccccctgat tttgctgcgg aaccgctttt accgccacta cacccaatgc
    43201 cgggaacacc agcgaaaacc cggcccccgc cagtaagacg ccgattttcg ccatccacgg
    43261 catagtcgcc acgccaacca gtagcaggcc gattatctca acgctaaagc aaatcatcgc
    43321 tacgtttaag ccaccgatac ggttaatgcc gttagggaat aacaaacgcg taccgacaaa
    43381 cgcacagcta aacagcgtca gcgcgaaagc cgcaccgtcc caacctttag cgtcataaaa
    43441 cagcgtgata aaggtggcga tgacgccaaa tccggcggaa gccagtgcca gcgccatacc
    43501 gtacagccag acgcgcccaa gcaccgcgcg aaacggcagc ggtttgcctt tactggcttt
    43561 taccgtcgga cgcgggatcg ccaacaaaat ggccaccagc gccacgccca taatgattaa
    43621 cgctaacgcc tgcaagccgc cccagtgata aaacacgacg cctaacggcg cacccatcgc
    43681 catcgccccg taagtgacaa tgccgttcca cgaaatcacc cgcccgatat gcagcgagcc
    43741 aaccactcca acgccccata gggtcgatcc cgttccggca aaactttgcc caatcccaag
    43801 gatgacgcgc cccaggcaaa gtaataacag gctgatgaca ggcagactgg cggttaatcc
    43861 tgccgtcaga taccccagac cgctcaaaaa gcagccgcat aaaccgaaga cgacaatctt
    43921 tttgggtccc agcaaatcgg cgtaacgtcc ggcatgaggg cggctcagca aggtggcgaa
    43981 atattgcagg ctgataacca atcctgccca gaaggcgcta aagcccatca catcatggac
    44041 atagcccggt aatacagcga gcggcaaccc gatggtgagg tagctggcga agttaaacat
    44101 gacaatggag acaatgcgca aattcaggcg caatccgttt agcgcgggtt cggctacggg
    44161 ttcgggcatg aggatcacca catttttaca acagtgtttc atttttacca tgtgctgacg
    44221 tgaaaatcag cagtaagaat cagaatattg ctggcgtgac tcccgcgcta cacttaacac
    44281 aaaaagtcac aaggaagccc caatggaaac ccctcaaccc gataaaacgg gcatgcacat
    44341 tctgctcaag ttggcctcgc tggtagtgat cctcgcgggc attcacgcag cggcagatat
    44401 cattgtgcag ctgttactgg cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg
    44461 gtttattcgt cggggagtac aacgccccgt tgccattacg attgtagtgg tggtgatgct
    44521 gatcgcacta accgcgctgg tcggcgtact ggcggcatcg tttaacgaat ttatctctat
    44581 gctgccgaag tttaataagg agctgacgcg caaacttttt aaattgcagg agatgttgcc
    44641 ttttcttaat ttgcatatgt cgccggagcg aatgctgcag cggatggact cggaaaaagt
    44701 ggttaccttc accacagcgc taatgaccgg gctttccggg gcaatggcga gcgtgctttt
    44761 gctggtgatg accgtagttt ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg
    44821 ttttgcgctg aataacccac agattcacat cgcaggatta caccgcgcac ttaaaggcgt
    44881 ttcgcattat ctggcgttaa agacgctact cagtttatgg acaggtgtca tcgtctggct
    44941 ggggctggcg ctaatgggcg tacagtttgc gctgatgtgg gcagtactgg cgtttttgct
    45001 caactacgtg cccaatatcg gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct
    45061 gctgtttaat ggtgtttacg aatgtattct ggtcggcgca ttgtttttag tggtccatat
    45121 ggtcatcggc aatattttag aaccacggat gatgggccat cgcctgggga tgtccaccat
    45181 ggtggtattt ctttcattgt taatctgggg atggctgctc ggcccggtag ggatgctact
    45241 ttcggtgcca ttaaccagcg tgtgtaaaat ctggatggaa acgacgaaag gtggcagcaa
    45301 actggcgatt ttactggggc cgggcagacc gaaaagtcgg ttaccgggat gaggcgacaa
    45361 gtgatacgat acgcactttc attttccatt aaacgttggc cctgatatgt atcggatagt
    45421 tctggggaaa gtttcgacct taagcgcagc tccactgcca ccgggtttac gcgagcaagc
    45481 accgcaaggt ccacgacgcg aacgctggct ggcggggcgt acattgcttt cgcacacgct
    45541 ttccccgcta ccggagatca tctatggcga acaaggcaaa cctgcattcg cgccggaaac
    45601 gccgctatgg ttcaacttaa gccatagcgg tgacgatatc gctctgctgt tgagcgatga
    45661 aggcgaagtc ggctgcgata tcgaagtgat tcgcccgcgc gtcaactggc gctggctggc
    45721 gaacgccgtg ttcagcctcg gggaacacgc tgaaatggac gccgtgcatc ctgatcagca
    45781 actggaaatg ttctggcgca tctggacgcg caaagaagcc atcgttaaac agcgtggcgg
    45841 cagcgcctgg caaatcgtca gcgtagatag cacctatcac tcctcgctgt cagtcagcca
    45901 ttgtcagcta gaaaatttaa gccttgcgat ctgcaccccc acccccttta cgctcaccgc
    45961 cgacagtgtg caatggatcg attcagttaa ctgatccgcc cacccgactg cccatctatt
    46021 gatccagaac aggtaatcag tatgacgaat acttaaaatc gtcatactta tttccgccat
    46081 ctattttaat ccattggggt taccatgctc tccacactcc gccgcactct atttgcgctg
    46141 ctggcttgtg cgtcttttat cgtccatgcc gctgcaccag atgaaatcac caccgcctgg
    46201 ccggtgaatg tcgggccact aaacccgcac ctttacacgc ctaaccagat gttcgcccag
    46261 agcatggttt atgaaccatt ggtgaaatat caggcagacg gttcggtgat cccgtggctg
    46321 gcaaaaagct ggactcattc agaagatggt aaaacctgga ccttcaccct gcgtgatgac
    46381 gtgaaattct ccaacggtga accgttcgat gccgaggcgg cggcagaaaa cttccgcgca
    46441 gtgctcgata accgtcaacg tcacgcctgg ctggagctgg caaaccagat tgttgatgtt
    46501 aaagcactca ataaaacaga gctgcaaatt accctgaaaa gcgcctacta tcctttcctg
    46561 caagaactgg ccctgccccg tcctttccgt tttatcgctc cctcgcagtt taaaaaccat
    46621 gaaaccatga acggaattaa agcgccgatt ggcaccggac cgtggatttt gcaggaatcg
    46681 aaactaaatc agtacgatgt cttcgtccgt aacgaaaact actggggcga aaagccagcg
    46741 attaaaaaga tcacctttaa cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa
    46801 actggcgata tcgacctgct gtacggaaac gaagggttat taccgctcga taccttcgcc
    46861 cgctttagcc agaatccggc ttaccacacc caactgtcac agccgatcga aaccgtgatg
    46921 ctggcgctca ataccgccaa agcccccacc aacgagctgg cagtacgtga agctcttaat
    46981 tacgcggtaa acaaaaaatc gctgattgat aacgcgttat atggcaccca gcaggtcgcc
    47041 gacaccctgt ttgccccttc tgtgccttac gctaatctcg gcctgaaacc gcgccagtac
    47101 gatccgcaga aagcgaaaga gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa
    47161 gacatccgcg agaaaaatgg tcagccgctg cgcattgaac tgtcgttcat cggcaccgat
    47221 gcgttaagca aatcgatggc ggaaatcatt caggctgata tgcgccagat tggcgcagat
    47281 gtctcgctga ttggcgaaga agagagcagt atctatgctc gtcagcgcga cggtcgtttt
    47341 ggcatgattt tccaccgcac ctggggcgcg ccatacgatc cacacgcctt cctgagttca
    47401 atgcgcgtac cgtcgcacgc tgacttccag gcacagcaag gattagccga caagccgctg
    47461 attgataaag agatcggcga agtgctggcg acccatgacg aaacgcaacg tcaggcgctg
    47521 tatcgcgaca ttctgacccg tctgcatgac gaggcggttt atctgcctat cagctacatc
    47581 tcgatgatgg tggtatcaaa accggagctg ggtaatatcc cctacgcgcc gatcgccacc
    47641 gaaattccgt tcgaacagat taaaccggtg aaaccttaat gttgcgttac gtattacgcc
    47701 gctttctgct gctgatcccg atggtgctcg ccgcctcggt gatcattttt ctgatgctgc
    47761 gcctcggtac cggcgacccg gcgctcgatt atctgcgttt atctaacctg ccgccgacgc
    47821 cggagatgct ggcctctacc cgcaccatgc tcgggctgga tcagccgctg tacgtccagt
    47881 acggtacctg gttgtggaag gcgctgcatc ttgactttgg tatctcattc gccagccaac
    47941 gcccggtact ggacgatatg ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg
    48001 cgctggtatt aattctgctc acttccgtac cgctcggtat ctgggcggcg cgccaccgcg
    48061 atcgtttgcc ggatttcgcc gtgcgcttta tcgcgtttct tggcgtgtcg atgcccaact
    48121 tctggctggc atttttgcta gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga
    48181 tgggctacgg cagctggcag cacatcattt tacctgcggt ttccatcgcc tttatgtcgc
    48241 tggcgattaa cgcgcgactg ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg
    48301 tcacctgggc gcggctacgc ggcctgaatg acaaacagac cgaacgccgc catattctgc
    48361 gcaatgcctc gctgccgatg atcaccgccg tggggatgca cattggcgaa ttgattggcg
    48421 ggacgatgat tatcgaaaac atctttgcct ggcctggcgt cgggcgctat gcggtgtcgg
    48481 cgatttttaa ccgtgactat ccggtgattc agtgctttac gctgatgatg gtggtggttt
    48541 ttgtggtctg taatttgatt gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc
    48601 gtcatgaagg agcgcacgcg tgaacttttt cctctcttcc cgctggtcgg tacgcctggc
    48661 gctgatcatt atcgccctgc tggcgctgat tgcgctcacc agccagtggt ggcagccgta
    48721 cgatccacag gcaattgatt tgccgtcgtg cctgctttcg ccggatgcgc agcactggct
    48781 gggcaccgat cacttaggtc gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc
    48841 gctcggttcg gtaatggcct gcctgctgct ggtgctgaca ttagggctgg ttattggcgg
    48901 cagcgccggg ttgattggcg ggcacgttga tcaggccacc atgcgcgtcg ccgatatgtt
    48961 tatgaccttc ccgacctcga ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct
    49021 gaccaacgta attatcgcca tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg
    49081 cagcttggtg atttcactac gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc
    49141 gggccatgtg cgggtgtttg tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt
    49201 gctggcaacg ctggatatcg gccatatgat gctgcacgtc gcggggatgt ctttccttgg
    49261 cctcggtgtg accgcgccga ccgccgaatg gggcgtgatg attaacgacg cgcgccagta
    49321 tatctggacc cagccgctgc aaatgttctg gccggggctg gcgctgttta tcagcgtgat
    49381 ggcctttaac ctggtgggtg acgcactgcg cgatcatctg gatcctcatc tggtgacgga
    49441 gcacgcacac taatgccgca acagattgaa ctacgtaata tcacgctaca ggccgcgcag
    49501 ccgctggtac acggtgtatc gttaaccctg caacgcgggc gcgtgctggc gttagtcggc
    49561 ggtagcggca gcgggaaatc attaacctgc gccgcgacgc tgggcatttt gcccgctggc
    49621 gttcgccaga cggcggggga aattttagcc gatggcaaac cggtttcgcc ttacgccctg
    49681 cgcggcatca aaattgccac catcatgcag aacccgcgca gcgcctttaa tccactgcac
    49741 accatgcaca cccacgcgcg ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct
    49801 acgcttaccg ctgccataga agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg
    49861 tacccgttcg agatgagcgg cggcatgttg cagcgcatga tgattgcgat ggcggtgctg
    49921 tgtgaatcac cgtttatcat cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag
    49981 gcgcgcatcc tcgatctgct ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg
    50041 gtcacccatg atatgggcgt tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa
    50101 ggtaaaattg tcgaacaggg cgatgtagaa acgctgttta acgcccccaa acatacggtg
    50161 acgcgcagcc tggtttccgc tcatctcgcc ctctacggta tggagctggc atcatgacct
    50221 tacttaacgt ctgcggcctt tcccatcact atgcccacgg tggatttagc ggaaaacatc
    50281 aacatcaggc ggtgctgaat aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc
    50341 tgctgggacg cagcggctgc ggaaaaagca ccctcgcgcg gctgctggta ggcttagagt
    50401 ctccctcaca agggaatatt agctggcacg gcgaatcgct ggcgaaactt aatcgcgccc
    50461 aacgtaaagc cttccgccgt gatattcaga tggtgtttca ggactccatc agtgccgtga
    50521 atccgcgcaa aaccgtgcgc gagatcctgc gtgaaccgat gcgccacctg ctatcactga
    50581 aaaaatccga acaactggcg cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca
    50641 gcgttctcga caaacgccca ccgcagttaa gcggcggcca gctccagctc gtctgcctgg
    50701 cacgcgcgct ggcggtcgaa ccgaaattgc tgattctgga tgaagccgtt tctaaccttg
    50761 atctcgtgtt acaggcgggc gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca
    50821 ctgcctgcct gttcatcact catgacttac gcctggtgga acgcttttgc cagcgagtga
    50881 tggtgatgga caacggacaa atcgccgaaa cccaggcggt gggagataaa ttaacctttt
    50941 cctctgacgc cggacgtgtg ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc
    51001 gcacctcaga aaaggtttaa cgccaatgca acgagtcacc atcacgcttg atgacgattt
    51061 actggagacg ctggacagcc tgagccagcg tcgtggttat aacaaccgtt ccgaagctat
    51121 ccgcgatatt ctgcgtagtg ccctggcgca ggaagctacc cagcagcacg gcacacaagg
    51181 tttcgcggtg ctgtcgtatg tgtatgaaca cgaaaaacgc gacttagcca gccgtattgt
    51241 ttccacccag catcatcacc acgacctctc cgtcgccacg ctacatgtgc acatcaacca
    51301 cgacgactgt ctggaaatcg ccgtgttgaa aggtgacatg ggtgacgtgc agcattttgc
    51361 cgatgacgtt atcgcccagc gcggcgtgcg gcacgggcat ttgcagtgcc tgccgaagga
    51421 agattgagtt atttctaaaa caaggaaaga aaagatatta actgaactta atttataaaa
    51481 ttaagttcag ttgcatagat agatcctgta aaggcagata ttctgcgtaa tgtacgtcgc
    51541 taaaatttat aaatgattca aacgctcatt cagtgtttca ataatatagg tattaacaga
    51601 gacttgttgc tgcgcagccg cattattaag gcgttcactc agcgattctg gatagcgtaa
    51661 cgtaaaggtc ttaatctttt ctgtacgcgc atacggctca atacccgcag ctttacaatc
    51721 ctcaagatac tctcgcaggg aaagctctcc ttctttttgc agcccctgaa tactgtcaga
    51781 gacaaaatca caataacctg acaagcccag gaatttgcca cgaaatgcat tcagttctgg
    51841 cacgtaggta ataacagctg gttgcccggc aatctccata gagtttggcg tcttcagttt
    51901 gatcatggtt ttacccctat acttagtaac cagtcacaca cgctttctac agcgccttta
    51961 tcggtatctg gcgacggatg aggccgatgg aaacacgcaa cgctcatatt tagtatgaac
    52021 ttgcaacgcg aacctcttcc ttccttaatt tctccgccta atgctttaac cagtgactct
    52081 atatcagacc acttgatccc ttgaggaaca ggagttttaa atatttgatc caatgtattt
    52141 ttttgtttct tccgtaaagc ctgtaccttg gttctcatgg catgtcctta tgacttcact
    52201 ttatgacatc acagtatgaa gtcaagtttc ccttccttac gccattgtcc caatcgtctt
    52261 cctgaatcgc agcagtgcga tggtgaaaaa cgcgccgcca attgccatca gcgtcaggaa
    52321 ctgcggccag acgatttcga atccggcacc ccggtagagg atggcctgcg cgaggctaac
    52381 aaagtgtgtc gtcggcatgg tcagcataat gtcctgcacc atctgcggca tactttcgcg
    52441 cggcgtggat ccaccggaaa gcatttgcag cggcagcagc accagaatca ccagcagccc
    52501 cagttgcggc attgaacgcg ctatcgtccc cataaaaatg ccgattgacg tggtggcaaa
    52561 cagactgagc gccacgccca gcataaacag cgggatcgag ccttcaatcg gtacgcccag
    52621 tacacctttc accatcagca ccagcgataa tcccgatacc accagcacca ccagccccat
    52681 cgaccagatc ttcgccatca tgatctcaaa cggcgttatc ggcatcacca gtaaatgttc
    52741 caccgtgccg tgttcacgct cgcggatcag cgccgatccg gtcaatacaa tcgccagcat
    52801 ggtaatgttg ttgatgatcg ccatcacccc gccaaaccac gcgggatcaa ggttcgggtt
    52861 aaagcgcatc cgggtttcca gcgataccaa cggttcgctg ttatcacggt agcgcgcgac
    52921 aaagctgttc acttcaccgt tgataatatt ctggatatac ccattgccgg taaatgcctg
    52981 gctcatgcgc gtggcatcga cgttcacctg aatatccggc tggcgtccgg cgaggacatc
    53041 acgctgaaaa ttaggcggaa tatttatcgc gaaggtatag cgtccggcgt ccagtccggc
    53101 atccatctca tcggcggtga tcatctccgg tggcaaaaac cacggacgat agaagctgtt
    53161 aacgatccgg ttcgataact gcgattgatc catatcggca atggcgatcg gcgcgaggtt
    53221 caacgatcct ggcgtaacgg tcgctgacga atacaccgac accgtaaacg agaagacaat
    53281 cagcgtcagc atcgctttat caccgagcag actgcgcaac tctttgatac ccagattaaa
    53341 aatattgcgt aaatggcgca tcatccctcc tgttttttca gcagcaggat gcttaagccc
    53401 atcaccagcg ggatggctat cagcaacggg ataaaaagtt gccacaaatc agtcagatcc
    53461 agcgctttcg agaacgttcc gcgggcgata gtcagaaaat gactggtcgg gtaaacctcg
    53521 ccgatccaac gtccaggccc ttccagcgaa gctaccggat cgatcatccc ggaaaactgt
    53581 gtcgccggga tcaatgtgat aatcgccgtt ccgaaaatgg cggcaatctg gcttttcata
    53641 aaggtggaga tcagcagccc cattccagtg gcaatgatga tatacagcag cgccgccaag
    53701 gtgagcgtca ggaaactgcc tttatgcggt acgccaaaca caaacaccga caggccgcag
    53761 agcaggaaaa agttcagcat ccccagcgcg atgtacggca gctgtttgcc aagcaaaaat
    53821 tcgctacggg tggtgggggt cacgtaaagg ttgataatcg accctagctc tttttcccgc
    53881 accacgctaa gggcgcttag cattgacggg atcatcatca gcagaagcgg gatcaccgcc
    53941 ggaacaatcg ctggcaggct ttttacgtcc gggttatagc gataacgcgt ctcaatgttc
    54001 atcagcccgc tttggctggc gggtgtcgat tgtcggctcg ccacatcctg aagccaactc
    54061 tggtgcattg cctgcacgta accttttacc gtttcggcac gactgggcat cgctccgtcg
    54121 atccagacgc cgagttccac aggcgtacca cgcgcgatat cgcgcccgaa attgggcggg
    54181 atctcaatgg ccaccgtgat atcgcccgca cgcatccgac gatcaagctc gtcataactg
    54241 gtgagcggcg gctgttcgat aaagtaacgg gaaccggaga ggttgagtgt ccacgcctgg
    54301 ctactgacgg tctggtcgcg gtcgagcacc gcaaagcgca ggttttccac atccatactg
    54361 atgccgtaac ccattatcag catcaggatc accgttccca tcagcgccag cgtcgaacgt
    54421 actggatcgc gtcgcagttc cagcgcttcg cggcggctgt agctaaacag acggcgcagg
    54481 ctaaatccct gacgcggcgc gtgggtggtg tcgtgtacca cgggcggcgc ttcggcttcg
    54541 ttgctctgcc ctgccgcttc ctgcaaatag gcgataaatg cctcttccag actggcggct
    54601 ccgcgtttct caaccagttc ctgcggtgta ccgctggcaa gcacttttcc ggcgtgcatc
    54661 agtgagatgc ggtcgcaacg ttccgcttcg ttcataaagt gggtggagat gaagatagtc
    54721 actttgtcct ggcgcgagag atcgaccatc aactgccaga acatatccct cgccaccgga
    54781 tcgacaccag aagtaggctc atcgaggatt aacatctccg ggcgatgaat caccgccacc
    54841 gccagcgaaa gccgctggcg aatgccgagc ggcaatgact ccggcagaac atcttcaacg
    54901 tcgttgagct taaaacgctc gctcatttca gccactcgcg cgggaatttc cgcttccggg
    54961 atgtgaaaca aacgggcatg taactccagg ttttgccgca cggtgagttc gttatagagc
    55021 gaaaacgcct gcgacatata gcccacacga cggcgggtat cgatatcttt tggatcaacc
    55081 ggttgcccaa atagccacgc ctcaccttcg ctggcgggca gcagtccggt aagcattttc
    55141 atggtggtgg atttaccgca gccgttcgaa ccaaggaatc caaaaatctc cccgcgtgga
    55201 atgcggaaat tgacgtgatc aacggcaacg aaggaaccaa aacgcatggt cagatcgcgc
    55261 gcttcgatgg caatctctgc gttttcaggt tgatacggtg ggatcactac cgcctgatgc
    55321 gcctggcgtt gcgcttgcgg taacagatta ataaatgctt cttccagcgt ggcgctttgc
    55381 gtttgctgcc gcagctcttc ggcgctgccg gttgccagca cttctccggc attcatcgct
    55441 accagccagt cgaagcgttc ggcctcttcc atataggcgg tggcgaccag cacgctcata
    55501 ttgctctgcc gctggcgaat actgtcgatc agatcccaga actgggcgcg ggagagcggg
    55561 tcaacccccg ttgttggctc atcaaggatc aacagttccg ggtcgtggat taacgcgcag
    55621 cacagcccaa gtttttgctt catcccgccg gagagtttac ctgccggacg atcgcgaaac
    55681 ggtgctaacc cggtgctggt cagcagctca ttaattcgcg cttcccgctc cgctttatcg
    55741 tgaccgaaca ggcgagcgaa aaaatcgaca ttttcataca ccgacaaggt gtggtagagg
    55801 tttttgccca gcccctgcgg catccaggcg atgcgcgggc agacgtctcg gcgatgcttc
    55861 gggtcgtgca tatcgccacc cagcaccacc acattgccct gctcaatgac gcgggcaccg
    55921 gaaatcagtg acaataaact cgacttcccg acgccatccg gaccaatcag cccgaccatg
    55981 caacgggccg gaatatcgag agtgatattg ttcagcgcaa cggtttttcc ataatgctgg
    56041 ctcacgcccg ccagttgcgc gacaggcggg acgggaacca gttccagatg cgtcattgcg
    56101 gcaacctcac cacgaggtcg tcaggccacg gaagttcttc attcacccgc acccacgcta
    56161 cgcccggtaa accggttttg acatattcca gatgctgctg gagtaattcc ggtgggatac
    56221 gcgctttgac gcggaacatc agtttcagcc gttcatcgct ggtttcgacg gtttttggcg
    56281 tgaactgggc gacactggcg acaaaactga tggttgcagg aatacgcaga tctggcgcgg
    56341 catcgaggat cagccgggct tcaccgccca gtttcagcgt gcccgcctgt tcggttggca
    56401 ggaagaaggt catatagacg tcgctgagat cgaccatatt cagcacccga ccgcctgccg
    56461 ccagcacttc ccctggctcg gcaacccgat actgcacgcg tccgtcacgc ggggctttca
    56521 gttcactgtc atcgatatct gcggcaatgc gccgttcagt ggcttgcgct gcttcgaccc
    56581 gagtttgcgc ctgaatgata ttggtgcgtg ctgcttctat agccgcttta gaagccgaaa
    56641 cctgggcttt cgctgattcc agcgcagctc gggcgctctc agcggcggcg cggtcgtcat
    56701 ccagctgttg cgcagaaata gcccctcgtt gggcgagcga gcgggaacgc gtatgacgtt
    56761 tggcaacaga gtccagttcg gcctggcgtt gattgaccag cgactgtgcg gcacgagttt
    56821 cactttgtcg ttgctccagc aaagcctgcg cggcggcaac ggcgctttgt gcctctttga
    56881 tttgcgcgat ggcttccagt cgctgttcct gcaacacgcg agtatccatc ttcgccagca
    56941 cttcgccttc gcgaacaaac tggccttctt tcaccagaat ggtgtcgata cgcccggcaa
    57001 ttttgctggc gatatccact tccgtcgctt caatgcgccc attgctgaca gcaaagcctt
    57061 ccggcacacc cgccgggcgc aacagccacc acgccacgac ggcagccacc gccagtgccc
    57121 cgacaaccca ccacgccaga tggcgcttac tcttatccat aatcgacccg ccataatccc
    57181 tgtaagcaaa cgacgcgtgt tacaactgcc gccgaacgac gttcagcgga ttcttccatc
    57241 cgggataaga aggaaacgac aaatacgacc agcgcgcaga gcattgagcg ccaacgggga
    57301 aggcttagtg aaaacgggta gtaatgttgt catcatcagc attcctggcc gtaaatgaaa
    57361 cacgcatact cataagtgtg atctataaaa ctggctgaca aaacggtatc catcctgttg
    57421 gatcgcattt cacgcctgtt cagttgcagg aacagagcat tcctttttac gttttatcaa
    57481 cagtctcaac cagatagtaa cattaatatt catgttgtct atggttcaga gctgtaacat
    57541 tgtgcgcctt ctaatcaaag gcataagtcc atttctgttt ttttcatgct ggtaagactt
    57601 ccagggagtg gttatctctg gccctgcacc agggttaatt tggcaattat gcatgctgaa
    57661 cattcttcca gcatgtttac tgtcacgggc gttaagacct aaaactgtcc ccgtttcacc
    57721 tatgcgttct ctcccaccat ttccgttatg tgcaacaact tgatttatac ctaacatttg
    57781 tgaacatgct aatagtgctt caagtttagg tctgtaatct gtcgaaacag aaaaataacg
    57841 attaaaggat cgacaagaga tagcccggct agttttaaac tttttgccat taattatagc
    57901 atgatgcttt ttattaataa tttccgctaa ttcgaccgga tcgagagcat tggtcatttc
    57961 atcaatagag acagttatcg gtcctataat gtaacgtttt cgctgatcat caaaagttat
    58021 cccatgatga tttgccatta ttttgtattt ggggtcatag aaacatacag gataatcttt
    58081 tatcccgtcg taggtatcct catcaagtgg tttcatgcgc gtgaagtttt gcatatagtt
    58141 accattgcaa ttcgtttcat gatttccagc taatacatga acattttcat tgatactcat
    58201 tatgcgtttt aacaaatcga tgataaattg atcgccacag atattactaa atcgatcgcc
    58261 ggtttggtcg ccaagaaacg tacatggcgt gactgcgtct ttattaataa ccaaataagg
    58321 ctgaagactt tctaaaacat tatctcttct gttcagtata ctgattattg tattaacatt
    58381 agtgtcaagt tgctcattaa agttctccct tagcaatacg ttgagaactt catcttctat
    58441 attaagcaac tggctaagga aattttctcc ttcagccatg attcccaaat gtcctgttgc
    58501 aaaaagcgcg tacaaaactg caccgactga gccatcggta tcgccaaaat aggtcggcct
    58561 ggtacttgtg ggatcaacag gtgaacaatt ggagaaatag gttctgtcag cgaactgggg
    58621 ttttataatt gtttcgaatt taatatcaaa tacattgtat tgcgatattt cccagttcca
    58681 gatgggtttg tacgactcag taactgcaac atcattagca ttatcattta ctgtcatatt
    58741 attaagtaaa taaacaatgc tgcaccaatt aatatcggta ttatcaagag ggcaaccgta
    58801 atttttacta ttaattgtca caataaactg ataacagtta ttcctacgta gaatatgagc
    58861 gcgcatgccc gatagcatac tatatttttc tgaatgattc gtttgtatgg taaatgaaat
    58921 tttgtcatct tgaatagatt gttggaaacg ttctatttgc caatcatcaa ttttttccac
    58981 aacttcaccg agcattgtcc tggcaatact tgatatatca tcacctatac catttgcaat
    59041 atacccaaac agttctttag tgaagttggt aacatgctct tgcaccatat catttgttgc
    59101 gattgtcgtt gtactgccat tagtgcctgc cacagttcca attttcatct tttacctcat
    59161 attattcacc gcgaggaata catagttaac attttatctt atgacataca cctcatattc
    59221 gatggggaaa taattatatt ttgcacaaat cattcagcct taaagcataa tatgaaattt
    59281 cttatctcat agcagagaaa gtccctaaat attgtagagg cgtcatcagt acatacctct
    59341 acaatcgatt aagaaaacct atgaaatact ggcaaaatta aaaagagata ccacagcact
    59401 catgaagttt tattgaggtt gaaaacaact aagccctggg acaccttttt tatcaccacg
    59461 ttcgttaggt gctatcttcc aagaatttag ccccatggta taattatcaa ttttttgatg
    59521 ctggtctacc cctaatccat aatggccaaa tttctgttta gtttttttga gcgtctgtcc
    59581 acatttgaag atatcttcag gttttggtct gaaagcagta gtgttattaa aataattttc
    59641 atatacaggt gtgggtgcta tagcattgct tctaattaga tggagaagat gatcatccat
    59701 gtgttgttta tgttttttat tacatatatt tgctaattcc tcaggatcaa ttgggtcttt
    59761 catctgatta aatggaacct gcaaagcacc gagacaataa cattgatttt cttcatttct
    59821 tattattccg tgatgactgg ttaaaacttt agtttctgag ttataattac aaacatcgag
    59881 cgttttaatt aagttatacg tatcaccggc agagagttta taattttcta gtcgtgcagt
    59941 atagttacca ttaaaattga tttcatgatt tccagccaga acgatgacat ttttattaat
    60001 acggctatct ttattaccct ccatatttcg catagaattt agcaaagtaa ggatatattt
    60061 atcaccaaaa atagtactaa atctatcgcc tgtatgatcc ccaaggaaga tacaaggggt
    60121 tatcgcatcg tcctttaaaa aaacatgttg atgcaactca ttcaatatca tgtctctcgt
    60181 atttaataaa ttgtagagca atttattatt tttatgtatg tatgcagatg aagccatgtc
    60241 ttcgtgtttt aataattcac ataataaact ccaacccttt tccctgatgc ctatatgccc
    60301 tgaaacgagc aatgaataaa gtacagcgcc tactcttcca tctgtatcgc caaaataagc
    60361 cgtgttggca ttttgtgggt taatcacacc acattgttta taaaagtcta ttttagaaaa
    60421 cacagattta acatagctat caaacttatc cttaggaaac gtctctttag agacatccca
    60481 acgccagtca gctttaaaat taccgctaag aagagtaaag aaattttcag gtatgataga
    60541 aagcccatta actttataaa ttatgtctga tttatctccg gagaaattaa taaaataata
    60601 accatcctta cactcaatct taactgtaat gggagacgat aattttatca catggttatt
    60661 gtctggtata attgtaaaga taaacgtgtt agtagtatta tcggcaaaac tattatattc
    60721 ttgaatatct atagatggct cactattgta aaaccatatt ttctccatct gaagactatg
    60781 ttcatttacc ctccagtgac catgttgcag gaaatagatg atatgcatgc caaaatcgtt
    60841 atctatttgc gaatcattat ccagcttatc aatttcaact atctttgaac gatgtgtttt
    60901 atcaaccttg ctatggtgtt gatgattatt aactttatct gaatgcttat ctttatggtt
    60961 tgaagggatg tacttaaata tattgtcaat tgctttcata aataacccgc gatatttttg
    61021 acctgttagt aaaaaataaa tgatacatgt ctgaaagaca tatttacgac gatataaaac
    61081 aataatactt atatgttcgc tgattttaag caccgagact atccggtctt ttactaaata
    61141 atggaaaaca atccatccct ggaagacaat tgtaaaaaaa acaacagaat aagaattaat
    61201 cttaggataa atatttattt atcatggcct tttgaccgct aaaaaatggc actcggcaca
    61261 gtaaaccccg ctatagttat gctatgcatt tgtctttaat gatatctcgt cgttatatta
    61321 acaggctgaa attatcatta atgcacattt caatattagc agggatacct gaggagttta
    61381 aagtgaacat atatatcggg tagcttttca aattaatccc tttgattata ggcttaattt
    61441 gcatcgcgtt aggtggcttt gtgctggaaa gttcagggca acgtcacggg tcatgtgctg
    61501 atttctctgg cggccatatg cctggcatta ttcactaccg catttattat catttcgcaa
    61561 ctcacgcgcg gcgttaatac gttttacaat acattgttcc ccattattgg ctatgcgggg
    61621 tcaattatca ccatgatatg gggttgggca ctgttagcag gtaatgatgt gatggcagac
    61681 gagtttgttg ccggccatgt tatttttggc gttggtatga ttgccgcatg tgtatcgacg
    61741 gtggcagcgt cgtccggtca ctttctgctc attcccaaaa atgcagcggg gagcaagagc
    61801 gacggaacac cggtacaggc ttattcttca ttaatcggta attgcctcat tgccgttccc
    61861 gttttactca ccctgctcgg tttcatttgg tctattacgc tgttacatag tgccgacata
    61921 actccgcatt atgtcgcctt gggttaaccg caatctgtgc ctgtctaatt ggcctagtgg
    61981 ccacaatcgt ccatcaaaca cgtaatacgt tctcatccaa agagcactgg ctgtggtgtt
    62041 attgggttat ttttctcggc tcagtcacgg tgctgcaggg gatatacgtc ttagtcagtt
    62101 ccgatgcaag cgcccgactg gctcccggca ttattctcat ttgcctcgga atgatctgtt
    62161 acagcatatt ctcaaaagtc tggctactgg cactggtctg gagacgtacc tgttcgttag
    62221 ccaacagaat accgatgata ccggtattta cctgtctgtt ttgcttgttt ttgtcatcat
    62281 tcctcgcgga aatcgcacaa actgagctgg actactttat tccatctcgg gttctggttg
    62341 gattaggcgc agtatgtttt acattgttct ctattgtttc aatattagag gccggttctg
    62401 cgaaaaaaaa ttaaaattta cataatccca gcgttattca tatcttaacg ctggtttttt
    62461 acgaacttag tgaaataatg taga
//
LOCUS       DAZSNG010000010        60420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZSNG010000010 DAZSNG010000000
VERSION     DAZSNG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60420
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.010"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..322
                     /locus_tag="ACHW4Z_000660"
     CDS             <1..322
                     /locus_tag="ACHW4Z_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8880429.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHW4Z_000661"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHW4Z_000661"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR8880430.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHW4Z_000662"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHW4Z_000662"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR8880431.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHW4Z_000663"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHW4Z_000663"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR8880432.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHW4Z_000664"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHW4Z_000664"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR8880433.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHW4Z_000665"
     CDS             complement(3726..4955)
                     /locus_tag="ACHW4Z_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8880434.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHW4Z_000666"
     CDS             complement(5019..5459)
                     /locus_tag="ACHW4Z_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR8880435.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHW4Z_000667"
     CDS             complement(5564..6334)
                     /locus_tag="ACHW4Z_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8880436.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHW4Z_000668"
     CDS             complement(6331..7257)
                     /locus_tag="ACHW4Z_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8880437.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHW4Z_000669"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHW4Z_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR8880438.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHW4Z_000670"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHW4Z_000670"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR8880439.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHW4Z_000671"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHW4Z_000671"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR8880440.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHW4Z_000672"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHW4Z_000672"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR8880441.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHW4Z_000673"
     CDS             12964..13311
                     /locus_tag="ACHW4Z_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR8880442.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHW4Z_000674"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHW4Z_000674"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR8880443.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHW4Z_000675"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHW4Z_000675"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR8880444.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHW4Z_000676"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHW4Z_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR8880445.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHW4Z_000677"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHW4Z_000677"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR8880446.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHW4Z_000678"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHW4Z_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHW4Z_000679"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHW4Z_000679"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR8880447.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHW4Z_000680"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHW4Z_000680"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR8880448.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHW4Z_000681"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHW4Z_000681"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR8880449.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHW4Z_000682"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHW4Z_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR8880450.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHW4Z_000683"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHW4Z_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR8880451.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHW4Z_000684"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHW4Z_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR8880452.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHW4Z_000685"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHW4Z_000685"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR8880453.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHW4Z_000686"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHW4Z_000686"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR8880454.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHW4Z_000687"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHW4Z_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR8880455.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHW4Z_000688"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHW4Z_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHW4Z_000689"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHW4Z_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR8880456.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHW4Z_000690"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHW4Z_000690"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR8880457.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHW4Z_000691"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHW4Z_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHW4Z_000692"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHW4Z_000692"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR8880458.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHW4Z_000693"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHW4Z_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR8880459.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHW4Z_000694"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHW4Z_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR8880460.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHW4Z_000695"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHW4Z_000695"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR8880461.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHW4Z_000696"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHW4Z_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR8880462.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHW4Z_000697"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHW4Z_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR8880463.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHW4Z_000698"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHW4Z_000698"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR8880464.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHW4Z_000699"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHW4Z_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8880465.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHW4Z_000700"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHW4Z_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR8880466.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHW4Z_000701"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHW4Z_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR8880467.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHW4Z_000702"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHW4Z_000702"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8880468.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHW4Z_000703"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHW4Z_000703"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR8880469.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHW4Z_000704"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHW4Z_000704"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR8880470.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHW4Z_000705"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHW4Z_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR8880471.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHW4Z_000706"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHW4Z_000706"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR8880472.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHW4Z_000707"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHW4Z_000707"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR8880473.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHW4Z_000708"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHW4Z_000708"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR8880474.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHW4Z_000709"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHW4Z_000709"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR8880475.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHW4Z_000710"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHW4Z_000710"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR8880476.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHW4Z_000711"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHW4Z_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR8880477.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHW4Z_000712"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHW4Z_000712"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR8880478.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHW4Z_000713"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHW4Z_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR8880479.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16733 c        14849 g        14365 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgctgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacact gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgatg
//
LOCUS       DAZSNG010000011        59875 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZSNG010000011 DAZSNG010000000
VERSION     DAZSNG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59875
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.011"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            546..1835
                     /gene="sdaC"
                     /locus_tag="ACHW4Z_000714"
     CDS             546..1835
                     /gene="sdaC"
                     /locus_tag="ACHW4Z_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR8880480.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1893..3260
                     /gene="sdaB"
                     /locus_tag="ACHW4Z_000715"
     CDS             1893..3260
                     /gene="sdaB"
                     /locus_tag="ACHW4Z_000715"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR8880481.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3371..4126
                     /gene="xni"
                     /locus_tag="ACHW4Z_000716"
     CDS             3371..4126
                     /gene="xni"
                     /locus_tag="ACHW4Z_000716"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR8880482.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="ACHW4Z_000717"
     CDS             complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="ACHW4Z_000717"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR8880483.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="ACHW4Z_000718"
     CDS             complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="ACHW4Z_000718"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR8880484.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6549..7865
                     /gene="fucP"
                     /locus_tag="ACHW4Z_000719"
     CDS             6549..7865
                     /gene="fucP"
                     /locus_tag="ACHW4Z_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR8880485.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7898..9673
                     /gene="fucI"
                     /locus_tag="ACHW4Z_000720"
     CDS             7898..9673
                     /gene="fucI"
                     /locus_tag="ACHW4Z_000720"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR8880486.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9782..11201
                     /gene="fucK"
                     /locus_tag="ACHW4Z_000721"
                     /pseudo
     CDS             9782..11201
                     /gene="fucK"
                     /locus_tag="ACHW4Z_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11203..11625
                     /gene="fucU"
                     /locus_tag="ACHW4Z_000722"
     CDS             11203..11625
                     /gene="fucU"
                     /locus_tag="ACHW4Z_000722"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR8880487.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11683..12414
                     /gene="fucR"
                     /locus_tag="ACHW4Z_000723"
     CDS             11683..12414
                     /gene="fucR"
                     /locus_tag="ACHW4Z_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR8880488.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12458..13558)
                     /gene="rlmM"
                     /locus_tag="ACHW4Z_000724"
     CDS             complement(12458..13558)
                     /gene="rlmM"
                     /locus_tag="ACHW4Z_000724"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR8880489.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13551..13946)
                     /locus_tag="ACHW4Z_000725"
     CDS             complement(13551..13946)
                     /locus_tag="ACHW4Z_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR8880490.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13965..14882)
                     /gene="gcvA"
                     /locus_tag="ACHW4Z_000726"
     CDS             complement(13965..14882)
                     /gene="gcvA"
                     /locus_tag="ACHW4Z_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR8880491.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15233..15460)
                     /locus_tag="ACHW4Z_000727"
     CDS             complement(15233..15460)
                     /locus_tag="ACHW4Z_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR8880492.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15652..16857
                     /gene="csdA"
                     /locus_tag="ACHW4Z_000728"
     CDS             15652..16857
                     /gene="csdA"
                     /locus_tag="ACHW4Z_000728"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR8880493.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16857..17300
                     /gene="csdE"
                     /locus_tag="ACHW4Z_000729"
     CDS             16857..17300
                     /gene="csdE"
                     /locus_tag="ACHW4Z_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR8880494.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17351..18157)
                     /gene="tcdA"
                     /locus_tag="ACHW4Z_000730"
     CDS             complement(17351..18157)
                     /gene="tcdA"
                     /locus_tag="ACHW4Z_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR8880495.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="ACHW4Z_000731"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="ACHW4Z_000731"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR8880496.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19701..19777
                     /locus_tag="ACHW4Z_000732"
     tRNA            19701..19777
                     /locus_tag="ACHW4Z_000732"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19735..19737,aa:Met,seq:cat)
     gene            19811..19887
                     /locus_tag="ACHW4Z_000733"
     tRNA            19811..19887
                     /locus_tag="ACHW4Z_000733"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19845..19847,aa:Met,seq:cat)
     gene            complement(19962..21215)
                     /gene="amiC"
                     /locus_tag="ACHW4Z_000734"
     CDS             complement(19962..21215)
                     /gene="amiC"
                     /locus_tag="ACHW4Z_000734"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR8880497.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21447..22778
                     /gene="argA"
                     /locus_tag="ACHW4Z_000735"
     CDS             21447..22778
                     /gene="argA"
                     /locus_tag="ACHW4Z_000735"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR8880498.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22840..24666)
                     /gene="recD"
                     /locus_tag="ACHW4Z_000736"
     CDS             complement(22840..24666)
                     /gene="recD"
                     /locus_tag="ACHW4Z_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR8880499.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24666..28208)
                     /gene="recB"
                     /locus_tag="ACHW4Z_000737"
     CDS             complement(24666..28208)
                     /gene="recB"
                     /locus_tag="ACHW4Z_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR8880500.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28201..31089)
                     /gene="ptrA"
                     /locus_tag="ACHW4Z_000738"
     CDS             complement(28201..31089)
                     /gene="ptrA"
                     /locus_tag="ACHW4Z_000738"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR8880501.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31265..34633)
                     /gene="recC"
                     /locus_tag="ACHW4Z_000739"
     CDS             complement(31265..34633)
                     /gene="recC"
                     /locus_tag="ACHW4Z_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR8880502.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34646..34996)
                     /locus_tag="ACHW4Z_000740"
     CDS             complement(34646..34996)
                     /locus_tag="ACHW4Z_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR8880503.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34981..35388)
                     /locus_tag="ACHW4Z_000741"
     CDS             complement(34981..35388)
                     /locus_tag="ACHW4Z_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR8880504.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35385..35948)
                     /locus_tag="ACHW4Z_000742"
     CDS             complement(35385..35948)
                     /locus_tag="ACHW4Z_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR8880505.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35939..36409)
                     /locus_tag="ACHW4Z_000743"
                     /pseudo
     CDS             complement(35939..36409)
                     /locus_tag="ACHW4Z_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36593..37387)
                     /gene="thyA"
                     /locus_tag="ACHW4Z_000744"
     CDS             complement(36593..37387)
                     /gene="thyA"
                     /locus_tag="ACHW4Z_000744"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR8880506.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37394..38269)
                     /gene="lgt"
                     /locus_tag="ACHW4Z_000745"
     CDS             complement(37394..38269)
                     /gene="lgt"
                     /locus_tag="ACHW4Z_000745"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR8880507.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38420..40666)
                     /gene="ptsP"
                     /locus_tag="ACHW4Z_000746"
     CDS             complement(38420..40666)
                     /gene="ptsP"
                     /locus_tag="ACHW4Z_000746"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8880508.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40679..41209)
                     /gene="rppH"
                     /locus_tag="ACHW4Z_000747"
     CDS             complement(40679..41209)
                     /gene="rppH"
                     /locus_tag="ACHW4Z_000747"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR8880509.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41562..41708)
                     /gene="ygdT"
                     /locus_tag="ACHW4Z_000748"
     CDS             complement(41562..41708)
                     /gene="ygdT"
                     /locus_tag="ACHW4Z_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR8880510.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41894..42583
                     /gene="mutH"
                     /locus_tag="ACHW4Z_000749"
     CDS             41894..42583
                     /gene="mutH"
                     /locus_tag="ACHW4Z_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR8880511.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42652..43365
                     /locus_tag="ACHW4Z_000750"
     CDS             42652..43365
                     /locus_tag="ACHW4Z_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8880512.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43503..43721
                     /gene="ygdR"
                     /locus_tag="ACHW4Z_000751"
     CDS             43503..43721
                     /gene="ygdR"
                     /locus_tag="ACHW4Z_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR8880513.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43829..44869
                     /locus_tag="ACHW4Z_000752"
     CDS             43829..44869
                     /locus_tag="ACHW4Z_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR8880514.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44902..46092)
                     /gene="lplT"
                     /locus_tag="ACHW4Z_000753"
     CDS             complement(44902..46092)
                     /gene="lplT"
                     /locus_tag="ACHW4Z_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR8880515.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46085..48244)
                     /gene="aas"
                     /locus_tag="ACHW4Z_000754"
     CDS             complement(46085..48244)
                     /gene="aas"
                     /locus_tag="ACHW4Z_000754"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR8880516.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48830..49861
                     /gene="galR"
                     /locus_tag="ACHW4Z_000755"
     CDS             48830..49861
                     /gene="galR"
                     /locus_tag="ACHW4Z_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR8880517.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49868..51130)
                     /gene="lysA"
                     /locus_tag="ACHW4Z_000756"
     CDS             complement(49868..51130)
                     /gene="lysA"
                     /locus_tag="ACHW4Z_000756"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR8880518.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51252..52187
                     /gene="lysR"
                     /locus_tag="ACHW4Z_000757"
     CDS             51252..52187
                     /gene="lysR"
                     /locus_tag="ACHW4Z_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR8880519.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52174..52866)
                     /gene="ygeA"
                     /locus_tag="ACHW4Z_000758"
     CDS             complement(52174..52866)
                     /gene="ygeA"
                     /locus_tag="ACHW4Z_000758"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR8880520.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52995..54413)
                     /gene="araE"
                     /locus_tag="ACHW4Z_000759"
     CDS             complement(52995..54413)
                     /gene="araE"
                     /locus_tag="ACHW4Z_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR8880521.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54728..55489)
                     /gene="kduD"
                     /locus_tag="ACHW4Z_000760"
     CDS             complement(54728..55489)
                     /gene="kduD"
                     /locus_tag="ACHW4Z_000760"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR8880522.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55519..56355)
                     /gene="kduI"
                     /locus_tag="ACHW4Z_000761"
     CDS             complement(55519..56355)
                     /gene="kduI"
                     /locus_tag="ACHW4Z_000761"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR8880523.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56642..57823)
                     /locus_tag="ACHW4Z_000762"
     CDS             complement(56642..57823)
                     /locus_tag="ACHW4Z_000762"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR8880524.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58078..59307
                     /locus_tag="ACHW4Z_000763"
                     /pseudo
     CDS             58078..59307
                     /locus_tag="ACHW4Z_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            59734..>59875
                     /locus_tag="ACHW4Z_000764"
     CDS             59734..>59875
                     /locus_tag="ACHW4Z_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8880525.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NSC"
BASE COUNT        14781 a        16065 c        14582 g        14447 t
ORIGIN      
        1 ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac ttactcatca actcatttca
       61 tttgttatat gaatgtttct taccaccctc acgcgacaaa tatcatcaca gttaatatgt
      121 catacaattt atgttgcaac acaaacgttt ccctattttc ataaaccgta ttttatcgct
      181 atgaaaaaga aattatcgcc atgattaact aaaagtattg attttttcag ttcaacctac
      241 atatattgcg cgccccggaa gaagtcagat gtcgtttaat gggcaaatat tgcccttaaa
      301 ttctctttta cttttgattt acagagtaaa gcgttgggat aatctatctt ccaagcagat
      361 tattgtattt gggatcaaga tcactgatag atacataact tgtgtgtatc tttccgccct
      421 caaattatta cggcggtaaa tgattaagcc atcgccgata gacagatttc atttttacgg
      481 tcaggcacct tcccgggctg aactggctaa aagctgaatt atttgcattc ctccaggaga
      541 aatagatgga aacgactcaa accagcacga ttgcgtcgaa agactctcgt agtgcctggc
      601 gcaagacaga caccatgtgg atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc
      661 tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc gctgatcatc atggctatcc
      721 ttgcgttccc gatgacgttt tttgctcacc gcggcctgac tcgcttcgta ctgtctggta
      781 aaaacccggg cgaagacatc accgaggttg tagaagaaca ctttggtatt ggcgcaggta
      841 aactgattac cctgctctac ttcttcgcta tctacccgat cctgctggtt tatagcgtgg
      901 caatcaccaa taccgttgaa agcttcatgt ctcaccagct gggtatgacg ccaccgccgc
      961 gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac catcgttcgc tttggtgagc
     1021 agatgatcgt taaagcgatg agtattctgg tattcccgtt tgttggcgta ctgatgctgc
     1081 tggctctgta cctgatcccg cagtggaacg gcgcagcact ggaaacgctg tctctggaca
     1141 ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg
     1201 tgttctcgtt caaccactct ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt
     1261 acggcgatat ggcagaacag aaatgctcga agatcctggc attcgcacac atcatgatgg
     1321 tgctgaccgt aatgttcttc gtcttcagct gcgtactgag cctgactccg gcagacctgg
     1381 ctgcggctaa agagcagaac atctcgattc tgtcttacct ggctaaccac tttaacgcac
     1441 cggttatcgc gtggatggct ccgattatcg cgattatcgc tatcaccaaa tccttcctcg
     1501 gccactacct gggcgcacgt gaaggcttca acggtatggt gattaaatct ctgcgtggta
     1561 aaggtaagtc tatcgaaatc aacaagctga accgtatcac tgcgctgttc atgctggtaa
     1621 cgacctggat tgttgccacc ctgaacccga gcatcctggg tatgattgaa accctgggcg
     1681 gcccaatcat cgcgatgatc ctgttcctga tgccgatgta cgcaattcag aaagtaccgg
     1741 caatgcgtaa gtacagcggt cacatcagca acgtattcgt tgtcgtgatg ggtctgattg
     1801 caatctccgc aatcttctac tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg
     1861 cgcttcctcc gttttaacgc gatgtatttc ctatgattag cgtattcgat attttcaaaa
     1921 tcggcattgg cccttccagt tctcataccg ttggaccaat gaaagcgggt aaacaattta
     1981 ccgacgatct gattgcccgt aacctgctta aagacgtgac ccgcgtggtg gttgacgtgt
     2041 acggctcgct ctctctgact ggtaaaggcc accacacaga tatcgccatt attatgggcc
     2101 tggcgggtaa cctgccggat accgtggata tcgattccat ccccggtttt attcaggatg
     2161 tgaatactca tggtcgcctg atgctggcaa acggtcagca tgaagtggag ttcccggttg
     2221 atcagtgcat gaacttccat gcagacaacc tttctctgca tgaaaacggt atgcgcatta
     2281 ccgcgctggc gggcgataaa gtcgtttaca gccagactta ctactctatt ggcggtggct
     2341 ttatcgttga tgaagagcat tttggccagc aggatagcgc accggttgaa gttccttatc
     2401 cgtacagttc agcagccgat ctgcaaaaac attgtcagga aaccgggctg tcactctctg
     2461 gcctgatgat gaaaaacgag ctggcgctgc acagtaaaga agagctggaa cagcacctgg
     2521 cgaacgtctg ggaagtcatg cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt
     2581 tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact acgccggatg ctggtcagcc
     2641 aggataaaac caccactgac ccgatggcgg ttgttgactg gatcaacatg tttgcactgg
     2701 cagtgaacga agagaacgct gctggcggtc gcgtggtgac tgcgccgact aacggtgcgt
     2761 gcgggattat cccggctgtg ctggcgtact acgataagtt tatccgcgaa gtgaacgcta
     2821 actcactggc tcgttacctg ctggtagcca gcgccattgg ttctctttat aagatgaacg
     2881 cgtcgatttc tggtgccgaa gtgggttgcc agggtgaagt tggcgtggcg tgctctatgg
     2941 cggcagctgg tctggcagaa ctgttaggcg caagcccggc gcaggtttgc atcgcagcgg
     3001 aaatcgcgat ggagcacaac ctcggtctga cttgtgaccc ggtcgcgggg caggttcagg
     3061 taccgtgcat tgagcgtaac gccattgcag cagtgaaagc ggtgaacgcc gcgcgtatgg
     3121 cactgcgccg taccagcgaa ccgcgcgtct gcctcgataa agttatcgaa accatgtacg
     3181 aaacaggtaa agacatgaac gccaagtacc gcgaaacctc tcgcggcggc ctggcgatga
     3241 agatcgttgc ctgcgattaa tctctcccag aggcctcgtt ttgcgaagcc tcttcctgtt
     3301 ttctcaacca gccatagcct gttccggcat cgaatgttac ccttatcgcc tgatctttaa
     3361 gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta tccgtcgcat
     3421 tcatgccgtt cagggttcgc cctgtgtcga aacctgccag catgcgctcg atcagctcat
     3481 tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg
     3541 ctggcgtcat cagcgtttac cagattacaa agcggatcga ccgccaatgc cggaagagtt
     3601 gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc
     3661 agccagcggc aacgaagccg atgacttagc cgccacactg gcggtcaaag tgacacaggc
     3721 cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac
     3781 attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga
     3841 atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga tcagcagttc
     3901 aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc tggtcgagtt
     3961 tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa
     4021 attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga
     4081 tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc gagccggata
     4141 cgccgcaaat cgtatccggc attatcacat cagcgcattt accaggcggt atggtaaagc
     4201 tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac atcatccagt
     4261 gccgcctgag ccagtgccga aatgtcttcc ttgcgtacac caacatcacg caaatgtggc
     4321 ggaataccga catcacggtt gagggcaaac accgcttcaa cagcggcatt acgcgcccct
     4381 tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg gtacttctca
     4441 ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt cgcaacaccg
     4501 tgtggagtgt tataaaacgc gcccagtgga tgcgccatac catgcaccaa ccctaaccca
     4561 acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc tccggcatcc
     4621 ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc
     4681 agtgcatcgg ttagtgccca cgcgccacgg gtaatatacc cctcaatagc atgagtgagc
     4741 gcatcaacac ccgtcgcggc tttcagcgcc ggaggcatac catccatcat gtcagcatca
     4801 ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct
     4861 tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt ggtggggatt
     4921 gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct acgcacatcg
     4981 gcaaactccg ggttgttgct gataatgcca attgctttac aagtatcctg tggagaacca
     5041 ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag cccttctttg
     5101 acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca
     5161 gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt tttatcggtg
     5221 acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc cccaacagca
     5281 ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat ccttctcctt
     5341 gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt ctccagcact
     5401 acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag ggtcgtcagg
     5461 taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc cagattcacc
     5521 tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt gagagccagc
     5581 gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat aaggcgcgca aggaatagaa
     5641 ttaccgccag ccgccgcaat catgtagtga atagcgggga tcgatcggtt aagaatggaa
     5701 actgccgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg tctgctttga
     5761 taggctgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc atgtttaccg
     5821 ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat gcctgtaggc
     5881 gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc ctggttcagt
     5941 cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt atttcgttcc
     6001 attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc acactattga
     6061 attagccgtt taattaccca ccatcttctt cctgattaac aagaaagaaa ttcacaagct
     6121 tatattttgt gacctagctc aactaatcac agtaaataac tgcaagttct ctttttataa
     6181 ccccgttaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt tccatttccc
     6241 ctcgccagaa atgcccgttt tcggcacaaa caattaatac ggtcatctgg tttgtgtttt
     6301 ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat ggtagtcaca
     6361 taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg ttttttattt
     6421 gaaaatgaat ccatgagttc atttcagaca ggcaaatatt caccgatatg aagcccgaac
     6481 tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc tgaacctaag
     6541 aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta gataaagatg
     6601 cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg ttttttcttt
     6661 gggcggtagc caataacctt aacgacattt tattacctca attccagcag gcttttacgc
     6721 tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat ttcattatcc
     6781 caatccctgc tgggatattg atgaaaaaac tcagttataa agcagggatt attaccggat
     6841 tatttttata tgccttgggc gctgcattat tctggcccgc tgcagaaata atgaactaca
     6901 ccttattttt agttggccta tttattattg cagccggatt aggttgtctg gaaactgccg
     6961 caaacccttt tgttacggta ttagggccgg aaagcagtag tcacttccgc ttaaatcttg
     7021 cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt
     7081 tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca gagcaattga
     7141 gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc atcgtggcta
     7201 tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg cagagtgata
     7261 atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgtctg gcgcgtattc
     7321 gtcactggcg ctgggcggta ttagcgcaat tctgctatgt cggcgcacaa acggcctgct
     7381 ggagctattt gattcgctac gctgtagaag aaattctagg tatgactgca ggctttgccg
     7441 ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc ggtacctggc
     7501 tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc gctatggcac
     7561 tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg actttatgca
     7621 gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag aatctcggcc
     7681 aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc ggtattgtca
     7741 ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct gaactgatcc
     7801 ccgcactctg cttcgcggtc atctttatct ttgcccgttt ccgttctcaa acggcaacta
     7861 actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa
     7921 aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga
     7981 acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac tgcgccatgc
     8041 ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta tggctgaagc
     8101 cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc ctcaccatta cggtaacgcc
     8161 ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga aagccatttg
     8221 gggctttaac ggcactgaac gccccggcgc tgtttacctg gctgcggctc tggcagctca
     8281 cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg atgccgatga
     8341 cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc
     8401 cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc
     8461 cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag tccaggcggt
     8521 ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag ccgaactgga
     8581 aatggcactg gcctgggctg ataaaaactt ccgctatggc gaagatgaaa ataacaaaca
     8641 gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac tgatggcgat
     8701 gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc gcgtggaaga
     8761 atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact ggaccgatca
     8821 atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact ggaatggcgt
     8881 gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat
     8941 gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct actggtcacc
     9001 agaagcaatt gagcgtgtaa cggggcataa actggacgga ctggcagaac acggcatcat
     9061 ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga
     9121 aggtaacccg acgatgaagc cgcactggga aatctctcag caagaggctg acgcttgcct
     9181 cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg gttactcttc
     9241 ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg
     9301 cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga aggatgtgca
     9361 tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg caccgcgcct
     9421 cactggtaaa gggccgttta cggatgtgta ttcggtaatg gcgaactggg gtgccaacca
     9481 cggagttctg accatcggcc acgttggcgc agactttatc acccttgcct ccatgctgcg
     9541 tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc
     9601 tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact acggtccgtt
     9661 gtacaagcgt taatacatta caccctgatg tgatgcccgg tcgctccggc taccgggcct
     9721 gaacaagcaa gagtggttag ccggataaac aatgttatcc ggctatattg caggagcgat
     9781 tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca gggccatcgc
     9841 ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc
     9901 gatggaaaac aacacctggc accagtggtc tttagactgc cattttgcaa cgctttgctg
     9961 attgctgtcg gcaaatcaat agtgaactga ctgaatgcca catccgcggt atcgccgtca
    10021 ccacctttag tgtggatggc gctctggtag ataagcaagg caatctgctc tatccgatca
    10081 ttagctggaa atgtccgcga acagcagcgg ttatggacaa tattgaacgg ttaatctccg
    10141 cacagcggtt gcaggctatt tctggcgtcg gagcctttag tttcaatacg ttatataagt
    10201 tggtgtggtt gaaagaaaat catccacaac tgctggaacg cgcgcacgcc tggctcttca
    10261 tttcgtcgct gattaaccac cgtttaacca gcgaattcac tactgatatc acgatggccg
    10321 gaaccagcca gatgctggat atccagcaac gcgatttcag tccgcaaatt ttacaagcca
    10381 ccggtattcc acgccgactc ttccctcgtc tggtggaagc gggtgaacag attggtacgc
    10441 tacagaacaa cgccgcagca atgctcggct tacccgttgg cataccggtg atttccgcag
    10501 gtcacgatac ccagttcgcc ctttttggcg ctggtgccga acaaaatgaa cccgtgctct
    10561 cctccggtac atgggaaatt ttaatggttc gcagcgccta ggttgatact tcgctgttaa
    10621 gtcagtacgc cggttccacc tgcgaactag atagccaggc atggttgtat aacccaggta
    10681 tgcaatggct ggcatccggc gtgctggaat gggtgagaaa actgttctgg acggctgaaa
    10741 caccctggca aatgttgatt gaagaagctc gtctgatcgc acctggcgcg gatggtgtga
    10801 aaatgcagtg tgatttattg tcgtgtcaga acgctggctg gcaaggagtg acgcttaata
    10861 ccacgcgggg gcatttctat cgcgcggcgc tggaagggtt aactgcgcaa ttacagcgca
    10921 atctacagat gctggaaaaa atcgggcact ttaaagccta tgaattattg ttagtcggtg
    10981 gaggaagtcg caacacattg tggaatcaga ttaaagccaa tatgcttgat attccggtaa
    11041 aagttctcga cgatgccgaa acgaccgtcg caggagctgc gctgttcggt tggtatggcg
    11101 taggggaatt taacagcccg gaagaagccc gtgcacagat tcattatcag taccgttatt
    11161 tctacccgca aactgaacct gaatttatag aggaagtgtg aaatgctgaa aacaatttcg
    11221 ccgttaattt ctcccgaact attgaaagtg ctggcagaga tgggacatgg agatgaaatt
    11281 attttttccg atgctcactt tcccgcccat tcgatggaac cgcaggtgat ccgcgctgat
    11341 ggcctgttgg tgagcgactt gctccaggcg attatcccgt tatttgagct ggacagttat
    11401 gccccgccgc tggtgatgat ggcggcggta gagggtgaca ctctcgatcc tgaagtagaa
    11461 cgacgttacc gtaatgcgct ttcactacaa gccccgtgtc ctgacatcat ccgcatcaat
    11521 cgttttgcgt tttatgaacg ggcgcaaaaa gcctttgcga tcgttatcac aggcgaacga
    11581 gcgaagtacg ggaatattct tttaaaaaaa ggggtaacac cgtaatctca taccggtacg
    11641 cccgcatgac gcgggcggtt atcgaatgat ggggtgaaaa atatgaaagc ggcacgccag
    11701 caagcgatag tcgacctgct gctgaaccat accagcctga ccacggaagc tctctctgaa
    11761 cagctaaagg tcagtaaaga aaccattcgt cgcgatctca atgaattaca gacgcagggt
    11821 aaaattctgc gcaatcatgg acgcgctaaa tatatccacc gtcaaaatca agacagtggc
    11881 gatccctttc acatcaggct gaaaagccat tatgcgcata aagcagatat cgcgcgcgag
    11941 gcgctcgcgt ggattgaaga agggatggtg atagccttag acgccagttc aacttgctgg
    12001 tatctggcac gccagttgcc tgacatcaac attcaggtct tcaccaatag ccatccgatt
    12061 tgccatgaac tcggtaaacg cgaacgcatt caactgatca gttccggcgg cacacttgag
    12121 cgcaaatatg gctgttacgt caatccctcg ctgatttccc aacttaaatc gctggaaatc
    12181 gatctgttta ttttttcttg tgaagggatc gatagcagcg gcgcactgtg ggactccaat
    12241 gcgatcaacg ctgattacaa atcgatgcta ttaaaacgtg ccgcgcaatc gttgttattg
    12301 attgataaaa gtaaatttaa tcgttcaggg gaagcccgca tcgggcatct ggatgaggta
    12361 acgcacatta tttctgatga gcgccaggtt gcaacttctt tggtaacagc ctgacggaaa
    12421 gggtagcagg ccggagacga cgccccggcc ttgcctgtta tcgctcgtcg cgacgaccac
    12481 ccaccgcagc ccagatgcgg cggacgtgca ccgtcacttc ttcgcgatcg tgatacaact
    12541 gccgcgcctg aatctgagca tttatgccat gttcatcaag ctgtgcctga atatacgcca
    12601 gattgtgtga tacttcttcg tagcgttttt tcatcggcag tttgagattg aaaatggttt
    12661 cacggcacca gccattaacc agccactgcg ccatcaatgc cgcaacttta gccggttttt
    12721 caaccatatc gcataccatc caggagatat tgctgcgtgt cggacggaat ttgaaaccgt
    12781 cttcccgcag ccacgtcacc tgcccggtat ccatcagact ttgcgccatc gggccgttgt
    12841 cgacggaata aacccacatg ttgcgcttca ccagttggta ggtccagccg ccagggcaag
    12901 cgcctaaatc caccgcccac atcccgttcg ccaggcgttc atcccactca tccgcaggaa
    12961 taaacacatg aaatgcctct tccagtttga gcgtggaacg actcggcgcc tctgccggaa
    13021 atttcaggcg cggaatgccc atatagaacg gcgaattatt gttgctgtat gagtaaccgg
    13081 tatagcagca tcctggtgca ataaagaata catgcacaac cggacgcttc ggcgtttcat
    13141 agttcgccag caccccagca tcgcgcaagg cagcgcgtag cggaacagtg aacttacggc
    13201 agaatttcag caactctttg ctttcgttgg tatcggcaac ttcaacacgc agttcaccgc
    13261 ccttctctac tacaccctgt aacatgccga caatgggggt aatacgatct tctggcggca
    13321 aatgctgcag gagttccccc accacaaacc actggcgggc aaaaattaat gaactgaacg
    13381 gcagctcacg gattaactta tcgccatcat caggttgata acattcataa atgacataac
    13441 ccgcattctc tttcacgcgg gcaaaaccga aaatttcccg ctggccggct ttatcggtaa
    13501 tttctgcggc gcactctttt tcaaagcccg gacggcacag caatacaacc ttattcatga
    13561 cttacgccct tgcgctttaa acggatagca ccaactaaca ttaacgccca gcccgcaagg
    13621 aagctcacgc cgccaaccgg tgtgacaaac gcccacaaac gcagatggga cagcgccagg
    13681 caataaaggc tgccgctgaa cagcaccgtg cctaacgcga ggaaaacgct actccagtaa
    13741 aaccagatac tgatgcgacg ctgcattgcc accgccagac ctaagatcgc cagcgtatga
    13801 aacgcctggt attcgagacc ggtttggatc cagcccatct caacggcccc catggtttta
    13861 cttaacacat gcgcaccaaa agcgcccagt gccacaaaaa tgaagccgct aatggcggcg
    13921 aaaatcagca taaaacggct ggtcatggtc gtaccctacg taaattattg ttcataacga
    13981 aagcggaatt tttcttgttc agcagcggct ttcgccagga tccattggcg aaaggcggct
    14041 attttaccca gttctgcctg gctgtcatga caaaccagat aaaaagcatt tttactgacc
    14101 agaacatcat taaacgggca aacaagacgt ccggcctcga tttcagattg cgccatcacg
    14161 ttatttgcca gcgccactcc ctgcccgtga atagccgctt gcagcaccat ggcgctatga
    14221 ctaaaaattg gcccttgctg aacgttgata tgattaaacc ccaactgtcg ggtatatgtc
    14281 tgccagtcac ggcgtgacgc atcatgtaat aacgtatgtt tagccagatc ttccggggtc
    14341 ttcaagggtt tttcgcctgt cagcagtagc ggcgaacaca ccggcaataa atattcggcg
    14401 tacagttttt ccacccgtag ccccggccag ttgccccgac cataaaatat cgccacatca
    14461 acatcatccg ccagcttatc ttcctgacga tcaaccgcct ggattcgaac gtcaattccc
    14521 ggataagctg aattaaagct ggaaagtcgc ggaaccaacc aatgaatggc gaaactgggg
    14581 agtaaactga ccgtcaacgc ccccttggcg ctacgggcct ggagtttacg cgtcgcttcg
    14641 gttaattgcg aaaatatctc tttgatatcg aggaaatagc tttgcccttc ctcggtcagc
    14701 aggagtgaac gattacggcg gcggaacagt tttagcccca aaaaatcctc aagagacttg
    14761 atttgatgac ttactgcggc ctgggtcaca aaaagctctt ctgctgcgcg agtaaaactt
    14821 aaatggcgtg ctgcggcatc aaaaactcgt aaggcattta gcggtggtaa tcgtttagac
    14881 atggctttta aactttgatg ttaaatgaat ttaacaatta gatcacactg tgtaacctat
    14941 tagttttttt aatctgagcc attataaatt gtccgttgag cttctaccag caaataccta
    15001 tagtggcggc acttcctgag ccggaacgaa aagttttatc ggaatgcgta ttctggtgaa
    15061 cttttggctt acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac
    15121 catggtagca aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga
    15181 ttaatgtagc accgcctaat tgcggtgctt ttttttacct tgcgatcgcg aattactgtt
    15241 ccagttcgac catctctttc acgtcagtac ggttgatctg ctgtttgttg ccattagcgt
    15301 ctttatacga aatcataccg atatcgttat cagtctgtgg tttgccgtca gagacgatgg
    15361 tacgtccgtc attggtgtgc atcacatagt tcgaaccgga acaggcgctc agggcaaaag
    15421 tcagcataca ggcagaaata attgcggcag tctttttcat catcttctcc ttaaaacgat
    15481 taataaatct taatagtatt gattaacagg ctaaaattaa cgcctaacac tattcagcat
    15541 atgttactta cgcggcttcg ccaggatatc cagataattc tgatggttag cactctcctt
    15601 gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt
    15661 tttaatcccg cgcagtttcg cgcccagttt cccgcactac aggatgcggg cgtctatctc
    15721 gacagcgccg cgaccgcgct taaacctgaa gccgtggttg aagccactcg acagttttac
    15781 agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg
    15841 cgttatgaag ctgcacgaga gaaagtggcg caattactga atgcaccgga tgataaaact
    15901 atcgtctgga cgcgcggcac cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt
    15961 ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg cagaacacca cgctaacctc
    16021 gtcccctggc tgatggtcgc ccaacaaact agagccaaag tggtgaaatt gccgcttaat
    16081 gcgcagcgac tgccggatgt cgatttgttg ccagagctga ttactccccg tagtcggatt
    16141 ctggcgttgg gtcagatgtc gaacgtgact ggcggttgcc cggatctggc gcgagcgatt
    16201 acctttgctc attcagccgg gatggtggtg atggttgatg gtgctcaggg gacggtgcat
    16261 ttccccgcgg atgttcagca actggatatc gatttctatg ctttctctgg tcacaaactg
    16321 tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg
    16381 ccctggctgg gtggcggcaa aatgattcac gaagtgagtt ttgacggttt caccactcaa
    16441 tctgcgccgt ggaaactgga agcaggaacg ccaaatgtcg ctggcgtcat aggattaagc
    16501 gcggcgctgg aatggctggc agattacgat atcaaccagg ccgaaagctg gagccgtagc
    16561 ttagcaacgc ttgcagaaga agcgctggcg aaacgtccag gctttcgttc attccgctgc
    16621 caggattcca gcctgctggc ctttgatttt gctggcgttc atcacagcga tatggtgacg
    16681 ctactggcgg agtacggtat tgccttgcgg gccgggcaac attgcgctca gccgctactg
    16741 gcagaattag gcgtaaccgg cacactgcgc gcctcatttg cgccatataa tacaaagagt
    16801 gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg aattattggt ggattaatga
    16861 caaacccgca attcgccgga catccgttcg gcacaaccgt aaccgcagaa acgttacgca
    16921 ataccttcgc accgttgtcg caatgggaag ataaatatcg ccagttgatc atgctgggga
    16981 aacagcttcc ggctttgcca gacgagttaa aagcgcaggc taaagagatt gccggatgcg
    17041 aaaaccgcgt ctggctggga tataccgtgg ctgaaaacgg caaaatgcac ttctttggca
    17101 acagcgaagg gcgcattgtg cgcggcctgt tggcggtgtt gttgactgcc gttgagggga
    17161 aaaccgccgc cgaattacag gcacagtcgc cgctggcatt gtttgatgag ctgggattac
    17221 gtgcgcagct tagcgcctca cgcagccagg ggttaaatgc gttaagcgag gcgattatcg
    17281 ctgcgacgaa gcaggtttaa gcacagaacc gggtcggata agacgttcgc gtctcatccg
    17341 acctgattgt ttaaccctga cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa
    17401 ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc gccaaatcca gatgcacaat
    17461 ccatccgctt cggcccttct gccgtggctt tcatcgcaca caccgtaccg tctgactgcg
    17521 ggtacaccag cgcttcagta gaaaacacgc aatccacgcc gagcttacct ttactgtttt
    17581 tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa
    17641 tcgttttcgc cagatcggta acctgaatct gcgtcgggtc aatctgccca cccgcgccac
    17701 cggtcgtgac cagcgggatt ttattgcgcc gacaataagc aatcagcgcc gctttgggcc
    17761 gtacactatc aatggcatca atcacgtacg aataaccggc actcatatat tgcgctacgt
    17821 tatccggcgt cacgaaatca tccaccaccg ttacacggca ctccgggtta atctggcgaa
    17881 tgcgctccgc catgacttcc gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt
    17941 gccgattggt attggtgacg cacacatcat ccatatcgat aagcgtgatt gcgccaatcc
    18001 ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc accgatacca accacacaaa
    18061 tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata cagacgcgct gtgccaccaa
    18121 aacgctgacg ccatgcatca ctaattacca cagacatagc acctcagaat taaaaagggc
    18181 gaggttatcc ctcaccctaa acgcataaat gcctgatgcg ctacgcttat caggcctaca
    18241 taactcctgc aatattttgg atttgcatga tttgtaggcc ggataaggcg ttcacgccgc
    18301 atccggtatg aacaacgcgc acttagtcaa taatcaggat gtgaggttac ccctcccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgtttcgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataggc tttagtggtt tgtttttgtt
    19621 caaaatcatg ctaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga
    19681 gtatagtgcg catccacgga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac
    19741 ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatc aaatttgatg aagtaaaagc
    19801 agtacggtga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg
    19861 tcggttcaaa tccggccccc gcaaccaatt attgaacacc ctaacgggtg tttttttgtt
    19921 tctgatctcc cataaaaaaa gcgccattca gcgccttttt atcatcccct tctcgccagc
    19981 gttgccccat cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc
    20041 tggaaagtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg
    20101 accagaatgg agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga
    20161 tttttatgca gcttgttgat ttttccgagc ttattcagta ccgctttacc aaacttaagg
    20221 ctgtcagcaa tagtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca
    20281 ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt
    20341 gccgcagtac tggttgcgcc tttggttgag agcgcaaaca ccgaggaacc gctcggctga
    20401 cgactggtaa aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct
    20461 tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc
    20521 atattgccct cttcctcgat cagagagcgc aaacggcgag ctatttgcaa taccacatct
    20581 ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca
    20641 agcataatga caatcggacg atcgcgccct gctttacccg gttgtggacc actttgcgct
    20701 ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg
    20761 tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg
    20821 gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatg
    20881 cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca
    20941 cgaatttgtg ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc
    21001 accacgcgtt cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct
    21061 accgtcacgc gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctaacc
    21121 gcagccaggc tgacctgact tacgctcaat agccacatag cgcccgcgcc ttgcagtaaa
    21181 cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta
    21241 agctgtgtaa caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct
    21301 gtcatctaat aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg
    21361 ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataattt
    21421 caaataatca tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg
    21481 gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc
    21541 tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt
    21601 tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa
    21661 atctggctgc gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca
    21721 aaacactgga actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc
    21781 tgtcaatgag tctcaataac acgccgctgc agggcgcgca tatcaacgtc gttagtggca
    21841 attttattat tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc
    21901 gtatccggcg gattgatgaa gacgcgctcc atcgtcaact ggaaagcggt gcaatagtgc
    21961 taatggggcc ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga
    22021 ttgccactca actagccatc aaactgaaag ctgaaaagat gattggtttt tgctcttccc
    22081 agggcgttac taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag
    22141 cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc
    22201 gtggcgcagt gaaagcctgc cgcagcggcg tgcgccgctg tcatttaatc agttatcagg
    22261 aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga
    22321 tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt
    22381 tgattcgccc gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga
    22441 tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc
    22501 tctatccatt cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt
    22561 accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga
    22621 gcggcttaag caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg
    22681 gatttacccc ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc
    22741 agcgtaaatc caaagtgttg atggcggatt tagggtaagc gatgaaaatc accggatgcg
    22801 gcaggcgtaa cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa
    22861 cgccgccaga ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc
    22921 atcggcatac agcgacagac gacgacgcgc gcgggtcacc gcggtgtata ccagctctcg
    22981 cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc
    23041 ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg
    23101 cacagactta atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc
    23161 cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat
    23221 caccggtcgg ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg
    23281 cataaactgt tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc
    23341 gcacaaaagc tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg
    23401 cagcagatcc agataacgtc cgtaacccgc aagagcttcc tcaagcatcg caatataatc
    23461 ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt
    23521 cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct
    23581 gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt
    23641 gccggttcct gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc
    23701 ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga
    23761 ggccagttga tcacgatcgc cgagaaagat cactcgcgca tgatctggca aggcgtcgat
    23821 cagtctcgac atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag
    23881 atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa
    23941 tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa
    24001 ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc
    24061 agccagacgg atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt
    24121 cgccacggtg gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt
    24181 cagcgccact gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt
    24241 gtccagggtt tgcgccagta gagcttcatc aacctcaatg gtatgattca cctcattgaa
    24301 gaagcgcgct accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca
    24361 gaggatcatc ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc
    24421 ttcccaattt tgtagctcac cgatttcact gacacaggtc gccaacagcg gattcgacgc
    24481 ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact
    24541 taacagtgcc gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa
    24601 ttgcacatcc agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa
    24661 tttcattacg cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac
    24721 ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc
    24781 aggaacagat agataacgcc gccaaagtga agatcatagt cgtaatcagc aatgcgatgg
    24841 cgcagataac gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc
    24901 tgcatcgctg ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg
    24961 gatttatagt cgagcaggta ataacgccct tcgtggcgga ataccaggtc gataaagcct
    25021 tttaacatgc cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca
    25081 aactggcgaa ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa
    25141 aactccatct ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccgtttca
    25201 ttgagaggtg cctggaggac agccgtgatc cactcggtca acaccggttc ccactgcgat
    25261 tcaaagccgc cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa
    25321 tcgaggtctt caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga
    25381 tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc
    25441 cgtggcatca gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg
    25501 acacgccagt tatcgccggg caatcgttgt agtgtcctcg cattcagttc tgcagttaaa
    25561 gcatcattaa cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca
    25621 tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt
    25681 tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga
    25741 cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg
    25801 taaagcaaac gcagatcttc cgccagacgt tcgacctccg cgaggtcgac gctttctggc
    25861 gcagcattaa gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc
    25921 tggacgcgga aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat
    25981 ttatggatcg tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg
    26041 gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca
    26101 ctttccagct gcgttccggc ttcttgaagc agttcgctga tatgcaagat atcggtaaga
    26161 cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc
    26221 gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct
    26281 tcgactaccg catcccacgc atgttcgtca ttattcagcg tttcaatatc cagcgcattc
    26341 agccccatca ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc gggcgtcatc
    26401 accgcctgca acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg
    26461 ttcgaaaggt aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc
    26521 tcctggcggc tgcgcaccag cacgctgatg tccgaagcac gcaccggacg cgcgtcgtcg
    26581 ccgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca
    26641 gcacatacct gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc
    26701 agccacattt tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt
    26761 ttcccggctg atttcactgg aataaacggt atttcgcgaa acatgaacgt gtcatcagtc
    26821 tggctgaaaa gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct
    26881 aaagtgtagt gggcatgaac ttcactacgc gccttcatat aagtgaagat atccgcaccc
    26941 cggaatgcat atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga
    27001 tggtgccaga tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg
    27061 atcattgcca ccgggaatcg cgtacggatc gccgctgcca acacctcgcc gctttcgcta
    27121 cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt
    27181 ttttcacgcg ctactgtttc gcggatctca gccaatgcgc gggtgatcaa cagatcgcgg
    27241 atcgacaatg gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt
    27301 tcccccccgg ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc
    27361 ggcaactgat aactgtttgt ctcttcttct gcccaggcgc tgatcttttc gatccattta
    27421 gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc
    27481 agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc
    27541 gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcggttcg
    27601 ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag
    27661 acgacctggg ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag
    27721 gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat
    27781 tcaaaggcat tcaggttgag catgcgctgg caaaagccgt gaatagtaaa gactgccgct
    27841 tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc
    27901 tcttccagca ggcgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc
    27961 aactcgtgga tattgctacg gatacgaccg cgcaattctg ccgtggcagc ctcggtaaag
    28021 gtgaccacca gcagttcttc aacgatcagc gggcggggaa aggcggcgga accgcctagt
    28081 ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag
    28141 gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca
    28201 tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca
    28261 cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg
    28321 cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt
    28381 ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt
    28441 gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg
    28501 cagcatctgg gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg
    28561 caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg
    28621 tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa
    28681 cgcaaacacg gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg
    28741 tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg
    28801 tacaaatacc gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga
    28861 ttgtttttta tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa
    28921 ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt tgccgataac
    28981 cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa
    29041 cgtaatggag ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag
    29101 catctgcgcg ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat
    29161 ctggttatac caggacttcg cctgctcaag ctgctcttcc gtagcggtat agctaaagta
    29221 cccctcgagc aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaat
    29281 cataaggccg ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct
    29341 taactgatca agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg
    29401 ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcagctt tgggctcgct
    29461 ggcaaaataa cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc
    29521 tggatggtcg tatttcttct ctgacttaat cagcgagaaa tcatcaggaa tataagggtt
    29581 aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcgg cgaaagtttg
    29641 tgcgctgatt ttatcgacct gatacggcgc atcgacaaag taagccgttt tgttgtgcgg
    29701 ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc
    29761 ctttactgct ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac
    29821 aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgatcga
    29881 cggataacgg aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat
    29941 gcctttttca cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt
    30001 agccaggcct ttatcagtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac
    30061 gataggatcg gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcaga
    30121 aagtgtacct gggctgcgat tgccaatcag ataggtaatc aattcatcgg ttttactacg
    30181 gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc aacactttac gcggcagcgc
    30241 agggacgtaa tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc
    30301 cggttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa
    30361 ctccggcagc ggtttattac tgtaaataac cgccttcatc aaattggcgg aatagtactt
    30421 ctcgtggaaa tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc
    30481 gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac
    30541 ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcactgcatt
    30601 acgctcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcat cggccaggcg
    30661 gtctaccgca ccgggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg
    30721 cgcagtgctg gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc
    30781 ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg
    30841 gtacgcctcg ggatcttcca gcgacccaac gggcactacc agcgccgaga gcgatttaac
    30901 tgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata
    30961 ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc
    31021 tgcctgactt aatggtgccc agagggcaac aaacaacaat aatgctttga accaggtgct
    31081 gcggggcatt cacggacctc ataatcaact ttattttctg gacagattca acaggttaac
    31141 gcaaatcatc tgccagaatt taatcttgtg ctgcacgagc cagcctatgt ttatataacc
    31201 atcagtccgt gactggtgcg catcataaag taagcagata gattgcgcaa tttttataca
    31261 acactcatga ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga
    31321 tggcctccat tgtctctggt gttaattgcc gccagagccg ttgataccag atatcatcac
    31381 cttcgccacg caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt
    31441 gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc
    31501 acgcgccgcc actttcaggt aacaccagca atggcgcgga cattccttca cgatacccct
    31561 caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact
    31621 caccgtcttt gcgtagaaaa aggcgacttt cgccattacc accgctggca cagtagacaa
    31681 ggtgttccag ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca
    31741 acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga
    31801 gatcaatttc catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct
    31861 gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc
    31921 ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca
    31981 ataactgctg attgatttga taacggctaa gtccttccag aataaatggc tcggtgtctg
    32041 ggatttcgct gtcttcagta tggaagttca cctgcaaacg catctggaag aacgcccgca
    32101 ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg
    32161 gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt
    32221 cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac ggcatccggg
    32281 tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt
    32341 catcgcccgg tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg
    32401 acgggaaacg ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt
    32461 gctgcgcgga aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt
    32521 cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat
    32581 aaacgccgtc gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca
    32641 tcagagtaca aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca
    32701 gacgctgtgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac
    32761 ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg
    32821 tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt
    32881 ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag
    32941 ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt
    33001 gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc
    33061 gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc
    33121 cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga
    33181 tgtcaaaccg cgccgccagc accggcacat ccagcagcgc cagcacatcc tctgacacaa
    33241 agcgactgtc aggcagtgat aacaggctga taaacgcttc cagtaccgga tgtgactgcc
    33301 gcgcacgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca
    33361 ctgcctgaat aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg
    33421 gagtaagtgt cggggcttcc tccagcatcg ccagcaagcg atcgtgtaaa acttcaactt
    33481 cacgctgcgg gctatggcaa acgtggaatg tgatactgct atccagtgga tcaagcgggc
    33541 gtttgttatc gctacgggaa aactcttcga tgttcacacc cgcaacggcg cggttttcca
    33601 gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtcaca tcgacaaaag
    33661 cgtccagctc ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct
    33721 tcccccatga ggccagcagc gagttgccga catcctgttc accatcgctg ttaaagagct
    33781 gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac
    33841 gctgacgagt cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac
    33901 ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct
    33961 ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg
    34021 gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt
    34081 gccagcgcgg ttgtccgagt tcatgggtat attccaccag cgccttccac aacggggctt
    34141 gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc
    34201 agtccggacg atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga
    34261 acagttttcg cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc
    34321 gctccagcaa ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg
    34381 cgctctcttt ggggatttcc ggtaacaccc ggacgaacat atcccagata aagctcgctg
    34441 gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc
    34501 actgcgccat accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc
    34561 gttcgcgttc gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt
    34621 agacccttaa catagcggct cctgactact gacaattcgg gcaatgcagg cgggtcatct
    34681 cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg
    34741 atgtctgcat tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt
    34801 gctgccaacc atgccgccag agcttttggt actggtttct gctggcgaga ctgttcattg
    34861 atgttcgctg gatacccgat aacgcagtga caatcatcac catcagcacc atcagcacca
    34921 tcagcaccat cagcaccatc gcaaccatta cctccggcag gctaaagcct tgttgattct
    34981 tcagggaagc tgacataatg ccccctcttt cagcggacaa aaatcgctcc agccgcgtgg
    35041 cgaaaaaaca atttttccat caatgacttc gcctgttcgc cacaacgaaa cgccttcata
    35101 accggcaatc aataaggctt cattatctgc cagtaaacgc aaacaaacct gggcatcggt
    35161 tccagcgtac tgcgagcact gaactgctgg ctgcgtctgc caggagtgca tttttcccca
    35221 cgccagcgcc gactgaacga tggcctggcg gcgcaatgac tgactttcca tgctcacgcg
    35281 agaggcaaaa ctgcgatcct gctgactcat tccttgcaat agcaagctac ccaaaaccag
    35341 cagcatcagg accagagcca gtgacgaaac acctttttcg cggttcacag gttgaatcct
    35401 gtcacgctat agctggcatc caccacggtt tgcggttcag acttactggc agcacgcata
    35461 ttaaccgtca acaccggcga gaagccgctg acatcctgac gtacgacctg aaaagtgtcg
    35521 ataatgatgg catccggatt agtgacttta tcccagccct taccttcaca ggatgtcgca
    35581 ccgcgtagcg tttccagcac atgctccttc agacgaaatc caatctggtc ggactctttt
    35641 accggttcgc gatcccagat accgttactg ttcgcatccc actgcacaat gatacagtca
    35701 ccctgtccga caatttccag cccttcgccg gtacagctgc catgacaata acccgccctc
    35761 tggagatgct tcgcgacggt aaatacccgc agccagattt catcttccag tgccagctta
    35821 cgagtgctcg ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac
    35881 aatacgctac tgatcgccat agcaatcaac acttccagca gagaaaaacc ttgctctttt
    35941 acaggcatcc ttctgtttct ccttgctgat aaagccggag tcttccccac ggcgaaacca
    36001 ccagccacca ctcgcccgtt gagtttttga agcgaatatg cacggcccat gcggtattgc
    36061 gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg
    36121 gcacaaagac caatggtgaa ctgccatgac aggtattggc cccagcagcg gaactcacaa
    36181 ggcaccataa cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta tgccagttgg
    36241 catcttcacg taaatagagc aaatagtccc gcgcctagct ggcggtttgc caaagccgtt
    36301 gcgactgctg ccagtattgc cagccataga gtccacttgc gcttagcatg accaaaatca
    36361 gcatcgcgac cagcgtttca atcagcgtat aaccacgttg tgttttcatg ccggcagtat
    36421 ggagcgagga gaaaaaaaga cgagggccag tttctatttc ttcggcgcat cttccggact
    36481 atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga
    36541 gggtaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag
    36601 ccaccggcgc tttaacgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac
    36661 ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt
    36721 cgcggcttaa ttgcagatga gtttgatcca tatggttgct gtacaggtgc gtatcgccac
    36781 cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta
    36841 acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa
    36901 gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca
    36961 gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg cgcgaatccg
    37021 ggtcgttttt cagctggttc agtaccgtag taatctggtc aatatgacga ccatctggcg
    37081 ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccattt tcatcggccc
    37141 attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagta tcgccctgca
    37201 ggaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttggtt gtcaccagcg
    37261 ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc
    37321 cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact
    37381 gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca
    37441 tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca
    37501 cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg
    37561 cgccgtagcc aatcaggaac aagcctgaga cagctcccat tgggcgcggt ttacgaatat
    37621 acaggttgag gataataaac agcaccacac cttccagcag cagttcgtaa agctgtgatg
    37681 ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca
    37741 gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt
    37801 caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa
    37861 acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta gtacggcggg
    37921 cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt
    37981 cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa
    38041 cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa
    38101 cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc agccacattg
    38161 caaaaatgaa ccccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg
    38221 agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca
    38281 agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc
    38341 gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa
    38401 gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct
    38461 ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc agactacgct
    38521 gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt acccgcgcta
    38581 cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca
    38641 tgggatcgcc cgccatttca ccgcacaaac gaagatcgat accatgtatt tccgcttccc
    38701 gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcgtaa atgttcgcca
    38761 cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag
    38821 agatgaaatc gaccctcttt gccagatgcg gcagcataaa taccattgac ggcacttcca
    38881 gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc
    38941 cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg accatcggca
    39001 acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca
    39061 agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct
    39121 cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg
    39181 tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt
    39241 ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat
    39301 caatacagct gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt
    39361 ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc
    39421 taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat
    39481 cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag
    39541 gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat
    39601 gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag
    39661 caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg
    39721 gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcac
    39781 cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa
    39841 tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc
    39901 gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt
    39961 tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca
    40021 gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct
    40081 gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg
    40141 ccggtaatgc gcggattcgc gtctggcgat attgcccaaa cagggcagtc aactgcgact
    40201 gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt
    40261 catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa
    40321 taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga
    40381 agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc
    40441 cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc
    40501 gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct
    40561 cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg
    40621 gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt
    40681 aacctctttt acgtcgataa gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg
    40741 acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca
    40801 ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac
    40861 tgctggtttg catattgatt tctgcatcgc cgcttaccag ctgcaagaga aaccattttt
    40921 gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt
    40981 agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt
    41041 cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt
    41101 gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat
    41161 tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc
    41221 aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt aaaccactct
    41281 ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc agagttataa
    41341 acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga
    41401 actacggaat tactacggga aaacccggag cattctgaat aagcccgata tagacatttt
    41461 aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc
    41521 gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtgttta
    41581 tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac
    41641 gtcaaggctg taaatgaaaa cagtagtgga ggtttttcac agttatccca gctttctgtg
    41701 gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag
    41761 tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac
    41821 acatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc
    41881 aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa ctgaagaaca
    41941 gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttga
    42001 cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga
    42061 gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt
    42121 ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg
    42181 tgttgcccgg ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa
    42241 gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc tggcgcagcg
    42301 tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga
    42361 agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg
    42421 tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc ttaccgaagc
    42481 cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa
    42541 tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc
    42601 cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt
    42661 gcatggataa ccgatcctaa cgcttggctt gcgctcggta cgctgacgct gctggagatc
    42721 gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct tcccacagca
    42781 caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg
    42841 ctggcatcaa tctcttgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag
    42901 gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc
    42961 agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca
    43021 tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag cctcgactcg
    43081 gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc cgtggtgatt
    43141 gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct
    43201 tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct gattctggaa
    43261 agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg
    43321 gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc cgtacgctct
    43381 cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc ctgctttcaa
    43441 gatatataac gtcggtttat aaacagacta ttatcatagg taggccaaat gaggataacc
    43501 ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct gtttccgggt
    43561 gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc gatggaaaac
    43621 ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt aacgcgatgc
    43681 aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg tcagcatccg
    43741 gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt tatattcctt
    43801 atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc acagttcgct
    43861 ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca gcgaagccga
    43921 cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg acgttgccga
    43981 aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct acgtcggcaa
    44041 ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag tgagcggacc
    44101 gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga agaatatccg
    44161 cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc tttatcaggt
    44221 gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct ggacggattc
    44281 tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc aacgcgcggg
    44341 aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc gctacctgca
    44401 tctggcggac aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt
    44461 aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg tcgaactgct
    44521 ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc
    44581 cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg agcaaacgca
    44641 aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggccttgatc ctgctcagat
    44701 ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac
    44761 gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg aagacgtatt
    44821 agctgaaatt gaagcggtac atcaggttta tacttatcct gcaccataaa gattaacgcc
    44881 ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg ccgttattgc
    44941 cagcgcaaat agcgcaccga agccaatgcc aatgggcacg accgggatgc ctaccattac
    45001 cgccagcgag taaatgccca gcatcaacaa catggcgctg ttttcgccaa ggttttgtac
    45061 tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca acgcattgag
    45121 cggaacgaca aaaaagcccc ccagcacgcc aatcagcatc aacaaggcat aggctggcag
    45181 cagctcgtgt tgcagggaga aaatcggtac caccacgcca atcaaaatcc cggctggcat
    45241 acagcgtgac acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc caaccacgat
    45301 accaatcgct accatcgcgt tgagataggt cggcgtagcg ttatcggtaa tgcccagcgc
    45361 caccggtacc cacagcacca acaggaaacg cagcgtgaca cccgctcccc agaataaact
    45421 ggtgcccacc agcgaaaaac gcgtttcacc attgcaccat agcgaggtgc aggcattcag
    45481 gaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg ccagtttggg
    45541 aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagagcgac
    45601 caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat
    45661 ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc
    45721 gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc
    45781 gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca ggccgttggc
    45841 aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgccaa
    45901 aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa
    45961 ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag
    46021 aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac
    46081 tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag
    46141 gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa
    46201 gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat
    46261 ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat
    46321 ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc gacaccattt
    46381 cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt
    46441 caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct
    46501 cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg ttcattatgt
    46561 tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgtg
    46621 catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga
    46681 tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt
    46741 tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc
    46801 gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg
    46861 taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg cggtaatgca
    46921 gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac aggcctaccg
    46981 tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt
    47041 cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta tggacgacgc
    47101 cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct
    47161 gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag
    47221 tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg
    47281 gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt
    47341 cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc atcattgcgg
    47401 gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca
    47461 gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca
    47521 gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt
    47581 tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg
    47641 ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc
    47701 gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc aaaatatgca
    47761 gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa
    47821 gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac
    47881 ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa
    47941 tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc ataggttgcg
    48001 tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttggc
    48061 ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca agcaaaatgc
    48121 catcaataaa agagacgtga ttaggcgtaa ttagtacgcg ctcgcccttc agtgcctggg
    48181 tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa
    48241 gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat
    48301 aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg
    48361 gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga
    48421 tcttgattgt ggtctgcacg acgactcttc gccagcgaga aaacgcaaag gaatgaaggg
    48481 aaatgcaacg aggtgtgtaa attgtcggtt actgttacag attgatgacc ggcaaaaaaa
    48541 aacctgcgca tctgcgcagg ctggtgtaat tcatgtgctc aacacgaagt tgacttcacc
    48601 tatcaatacc tctgggatca ccactttagc aacctgaagc caaacgccac cagcggtcaa
    48661 tcgcaacagc gtttcgcaaa gtgtaaccaa aggtttgcaa tctctttttt tgtcttactt
    48721 atttgcgttt ggctcacatt cccacgatga aaacacgcca ccccttgaac caacgggcgt
    48781 tttccgtaac actgaaacaa tgtaagcgtt tacccactaa ggtattttca tggcgaccat
    48841 aaaggatgta gcccgactgg caggcgtttc agtcgccacc gtttcccgcg tcattaataa
    48901 ttcacccaaa gccagcgaag cttcccggct ggctgtgcat agtgcaatgg agtctcttag
    48961 ctatcacccg aacgccaacg cccgtgcgct ggcgcagcag accactgaaa cggtcggtct
    49021 gatcgttggt gatgtttccg atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt
    49081 ggcttatcac accggtaatt tcttattgat tggcaacggt taccacaacg aacaaaaaga
    49141 gcgtcaggct attgagcaac tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa
    49201 aatgatccca gatgccgatt tagcctcatt aatgaaacaa atgcccggta tggtgctgat
    49261 caaccgtatc ctgcctggct ttgaaaaccg ttgtattgct cttgacgatc gttacggtgc
    49321 ctggctggca acgcgtcatt taattcagca aggtcatacc cgcattggtt atctgtgctc
    49381 taaccactct atttctgacg ccgaagatcg tctgcaaggg tattacgatg cccttgctga
    49441 aagtggtatt ccggccaatg atcggctggt gacatttggc gaaccagacg aaagcggcgg
    49501 cgaacaggca atgaccgagc ttttgggacg aggaagaaat ttcactgcgg tagcctgtta
    49561 taacgattca atggcggcgg gcgcgatggg cgttctcaat gataatggta ttgatgtacc
    49621 gggtgagatt tcgttaattg gctttgatga tgtgctggtg tcacgctatg tgcgtccgcg
    49681 cctgaccacc gtgcgttacc caatcgtaac gatggcgacc caggctgccg aactggcttt
    49741 ggcactggcg gataatcgcc ctctcccgga aattactaat gtatttagtc cgacgctggt
    49801 acgtcgtcat tcagtgtcaa ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta
    49861 accgcagtta aagcaattcc agcgccagta attcttcgat ggtctggcga cggcgaatca
    49921 accgcgcctg accattatca aacagaactt ctggtaacag cggacggcta ttgtagttgg
    49981 atgacattga tgcgccatat gcccctgtat catgcagtac cagataatca cctgccttca
    50041 cttccggcaa ggcgcgggtt tcaacatttc ccccttcctg ctgggtaaag acatcgcccg
    50101 attcacataa cggcccggcg acgacggttt ccaccgttgg cgcgtgttcc agagaacgac
    50161 catcagctgc cagggcactg atatggtggt aactaccgta cattgccggg cgcatcagat
    50221 cgttgaaccc ggcatcaacc agcacaaagt ggcggctacc catttgtttg acgctccgca
    50281 cctgagtaat taatacgcca gactgcgcta ccaggaagcg acccggttca atttccagtt
    50341 tcacagggtg gcccaaatgg cgagcgattt gctcacgcgc ggcattccac agaccataat
    50401 aatgttcggt atcaaccgcc tcttcaccct gttgataagg aatagaaagc ccaccgcccg
    50461 cagaaatagc ctgtaaatcc tgaccgaatt cgataacctg acgcaccata gcaccacaca
    50521 cctgctccag atgggcataa tcaacgccag aaccaatgtg catgtgaatg ccgactagct
    50581 gcagatgatg acgttgtatc acgtccagtg cggcgggcag atcggtgtac cagataccgt
    50641 gcttgctgtt ttcgccaccg gtattggttt tttggctatg tccgtgacca aaccccggat
    50701 taacgcgcag ccatacccga tgccctggcg aaacctggcc cagttggtcg agcgtatcaa
    50761 cagaacccgc attcaccgga atttgcaatt cactgacgcg ttcaagcgtc gcctgatcga
    50821 taacatctgc cgtaaaaaca atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca
    50881 acgcacgctc tatttcgcct aacgagacgg aatccacttt cacgccctgc tcacgcatta
    50941 agcgcaaaat atgaatattg gaacaggctt tctgtgcaaa gcgcaccact tcaaactgtt
    51001 tcagcgctgc aatctgccga cgaataattt gcgcatcgta gacccacacc gggcagccaa
    51061 attcagcggg caaacgcagc agattttcgg cggtgagatc ggtatcggtg ctgaacagtg
    51121 aatgtggcat aacaaactcc agatgagtgc ttttttatga ttacgccaca tcataaaaag
    51181 aataaaaaat atcgatttat gtcgagtcta tgcaaaaatg atatggatta ccggattgcg
    51241 agagagcgct aatggccgcc gttaacttac gtcatattga aatttttcat gcggtaatga
    51301 ccgccggaag cctgactgag gcagcacacc tgctacacac ctcacagcca accgtcagcc
    51361 gcgaactggc gcgctttgag aaggtgatcg ggctgaaatt gtttgagcgc gtacgtggac
    51421 gattacatcc taccgtgcaa ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg
    51481 gactggatcg cattgtcagc gccgcagaaa gtctgcgcga gtttcgccag ggagaactgt
    51541 ctattgcctg cctgccggtc ttttcgcaat cttttttacc gcagctcctg caaccctttc
    51601 tggcacgtta tcccgatgtc agcttaaata tcgtgcccca ggaatcaccg ctacttgaag
    51661 agtggctctc ggcccagcgt catgatttag gactcactga gacactccat acgcctacgg
    51721 gaacagaacg taccgaatta ctctctttag atgaagtgtg tgtgttacct ccgggtcatc
    51781 cgctggcggt aaaaaaggta ttaacgccgg atgattttca gggtgagaat tacatcagcc
    51841 tttcccgtac tgacagctat cgccagttgc tggatcagct atttactgaa catcaggtta
    51901 aacgacgcat gatcgtagaa acccacagcg ccgcgtcagt ctgcgcaatg gtacgggcgg
    51961 gggtaggtat ttcggtggtt aacccgctca ccgcactaga ttatgcggca agcggtttag
    52021 tggtgcggcg gttcagtatt gcggttccgt tcaccgtcag cctgatccgc cccctgcacc
    52081 gcccgtcatc agcgctggtt caggcgttta gtgggcattt acaagcgggg ttaccgaaac
    52141 tggtcacttc tcttgacgct attttgtcgt cagctacgac agcataaaag cgacagcatc
    52201 ctcggcatgg atcgccgcgg tatcaaacac aggcaaaaca ctgcgctctt ctggcaccag
    52261 taaaccaatt tctgtgcagc caaaaatgac gccctgtgcg ccctgttctg caaggcgagc
    52321 aatcacttgc gcataataag cgcgtgacgc ttcggtaaat tgccccagac acagttcttc
    52381 aaaaataatc tgattaattt tcgcccgttc atccgcttca ggaataagac aattgatgga
    52441 aaattgttcc gtcagccgcc cgcgataaaa atcctgttcc atggtgtaac gcgtacccag
    52501 cagcgccaca cgagtcattc cggccccggt aattgcacgt ccggtggcat ccgcaatgtg
    52561 taagaaaggc agagtgcaac gtgactcaat ggcatccgcc actttatgca tcgtattggt
    52621 acataacaca ataccttctg cgcccgcccg ctgtaagcca agcgccgcct cagccagaat
    52681 gtccccggtt ttatcccatt ccccgcgacg ctggcactct tctatttcat gaaaatcgac
    52741 gctatgtagc agcatttgcg cagagtgaag cccaccaagc cgctgtttaa tgccttcatt
    52801 tatcagacga tagtaaggaa tggtggattc ccagctcatt cctcccagca aaccaattgt
    52861 tttcattcct ctctcctgat gtgtgttacc gcaacaaggc tgaataagga acgagacaaa
    52921 cgcctcaacg gccaggtgcc caatctctat taacgaaaaa agggccggat gtacagcaca
    52981 tccggcccgt gaaatcagac gccgatattt ctcaacttct cgcctgccat cagtttgcgt
    53041 tcgatatgtt ccagcgtgac atttttggtt tccggaatga gccagaaagt aatacccaca
    53101 aacgcaatgt tcagcgcagt gtagagccag aacgtaccgg cagcgccaat gctatcaagc
    53161 agtgtcagga aggccgcgcc gataatcata ttcgacaccc agtttgtcgt cgtcgaacag
    53221 gtaataccga aatcgcggca tttcagcggc tgaatttcag agcacaggat ccacaccact
    53281 ggcgcggcgc tcatcgcata accggcaata cacatcatcg tcatgccaac agagagccag
    53341 gacaagccac tggaagccgt accgttatca aactgcatca ggcaatagcc cagcaccaga
    53401 gtgcctaacg ccatcacgct gaaaccaatt ttcagagccg gtttacgacc tgctttatct
    53461 accgtaaaga ccgcaatgaa ggtcgcgaac ataaaggtca gtcccacgac cagagtcgca
    53521 atcatctgtt gttctgtggt cgtaaagccc gccattttga agatacgcgg cgcgtagtac
    53581 atgatgatgt tcataccggt aaactgctgc atcgcctgca acaacatacc gagaaacaca
    53641 gcacgacgga cgttacggtt gatcttaaac agtgcccaac cgccctgttt taacttcagg
    53701 ctttcacgaa tttcgttgag ttcttctcgc gctttttccg acgtatcgcg cagcatacgc
    53761 aacacttctt ccgcctcaat atgacgcccc ttttccgcca gccagcgcgg gctatttggc
    53821 aggaagacca ccagaataat cagcagaact gctggtaaag caagaacccc caacattgcg
    53881 cgccagttac cgctataact gaacgctgta tcggataaaa acgccagcac gatgccgagt
    53941 gtgaccatca actggtacat actgatcatc ttaccgcgaa cgttttcact tgccatttca
    54001 gaaagataca gaggagcggt gtaagacgcg atcccgacag caatgcccag caccacacga
    54061 gcggcgatta acatctctac gctggtcgca aaagcggacc ctatagaacc gagtacaaac
    54121 aggatggccc ccgccatcag gctgtattta cgccccaggc ggaacgacag ccaaccatta
    54181 aacagcgcac caattgctgc gccgagcatc atgctactaa ccacccattc ctgcaaacga
    54241 ctggtcagca caaagtgatc ggtaatgaac ggcaacgctc cggcgattac gccgatatca
    54301 agaccaaata acaatcctgc gaccgcagca gctaccgaaa caaacatatt catacgccgc
    54361 gtatcacgca aagaacgtgg cgttaaagca gattccgtat tgatagtaac cattttttcc
    54421 tgccagcaga gagtaagaca ttgtgaaaaa atacgtgaac aattcacaca ggtgtcaggt
    54481 cggaaacagc ataaatatgg attaaattgc tgcgacatgc cgttatgtga tggatattcc
    54541 aattttaaaa ttaagttgaa ttattgagat tattattaac ctcctaattt taaagcagat
    54601 aaaattcata aagttcatta attgataatt gatatggatt atttcataac catgatatgg
    54661 attatgatga tctagagata taaaaaaccc tgccatgcgg cagggtcata aaagtaagag
    54721 gaatgaatta acgcgccagc caaccgccat ccacagcaat ggtataacca tttacataat
    54781 ctgaagcgct ggaggcaaga aacactaccg gccccatcag gtcactcggc agtccccaac
    54841 ggccagctgg aatgcgatcg agaatttccg cgctacgttg ttcatctgcc cgcagttgtt
    54901 gggtattgtt ggtcgccatg taacccggag ctatcgcatt aacattaatg ttgtgtttag
    54961 cccattcgtt tgccatcaat cgcgtcacac ccatcacgcc gctttttgat gcggtataag
    55021 aaggcacacg gatcccgccc tggaaggaga gcattgacgc gatattgata atcttgccgc
    55081 cattgccttg cgcgataaag tgtttcgccg ctgcctgaga catgaagaat acgctcttga
    55141 tattcaggtt catgacatcg tcccagtctt tttcgctgaa ctcgagagcg tcttcgcggc
    55201 gaatcaatcc ggcgttattc accaggatat caatatgacc aaattcagct accgcgcgat
    55261 ccagcagtcc aggaatgcca tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac
    55321 gccccagcgc cgtgacctgc tcgatggttt cagtcggttc aacgatgtta atgccaacaa
    55381 tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg gcccagccca gtatcacaac
    55441 cagtgacgac cgcaacttta ccttggagag aaaatgcact taaaatcata acaacacctt
    55501 attcgtttat gcccacaact agcgcaaatc tttaacgccc acatggtcca tatcattaaa
    55561 gacctggttt tcaccgacca tgccccagat aaaggtataa gctttggttc cgacgccgga
    55621 atggatcgac cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt
    55681 ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa
    55741 ataaacttcc atccggcgct cgtgggtgtg acacggcatg gtgttccaca agttacctgg
    55801 agccagctcc gtcagcccca tactcaattg acaggtttcc agtacatccg ggacaaaata
    55861 tttgttaatc gtgcgacggt tactggtgag gttatcgcct aacgtgactg gagatacttc
    55921 gtccggtgtg acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa
    55981 cttcgccgga gtggcggtat caatactggc aaagacaact tcttttgcac ctttaccaac
    56041 atacagggcg tcgcggtgac cgatttcata gcattgacca tcgacagtaa tcgtaccggc
    56101 accgccaata ttgataacac ctaactcgcg acgttcaagg aaatagctta cgccgagttg
    56161 tttaccaact tccccgccaa cggaaaccgt tttagttatc ggcataatgc cgccaacaat
    56221 aattcggtca atgtggctgt aaaccatggt gtactcatcg gcgacaaata ccttttcaac
    56281 caaaaattca ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac cgtggatgct
    56341 ctgtcttacg tccacatcaa cctccgataa tcagtgaagc gtgaaaatag aaaacgatag
    56401 tgaaacactg tttcgttttc tctataaagg gatgatatgc ccatgggcta gggttttcaa
    56461 ttaaaaataa aacaatgttt tatttttaat ttaaggatta aaaaaagtgc agtgtcgatc
    56521 acgaatatgt catgagaatg gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca
    56581 gccatcatta ttcaacagat taatgacggc agatatttat cggtaaggag gaaatgcaga
    56641 gctactcgtc acgttcaatg gtcaatgcca cgccctggcc cccgccgata caaagcgttg
    56701 ccagcccttt gcgggcatta cgtttcacca tttcatgaac cagagaaacc aggattcggc
    56761 aaccggaagc gcctatcggg tgaccgagtg cgatcgcgcc accattgaca ttgacccgac
    56821 gctcatccca ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag
    56881 cctcgataag atcgacatca gccaactgcc agcctacacg ctccaggcaa cggcgggtcg
    56941 catacaccgg cgcaattccc atcaatgccg gatctacacc aacgctggca aatgcgcgga
    57001 tgcgggccag cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc atcattaccg
    57061 cagctgcgcc atcgtttatg gatgatgcat tccccgcggt cacagaaccg agactatcaa
    57121 atgaaggatt taaacgggct aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg
    57181 tatcaacaac caacgtctga ccgttacttt ggatcattac cgggacgatc tcatctttaa
    57241 atcgtccggc gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg taagcatcct
    57301 gcaactgacg gctgatgcca tattcgcgag ccagattttc ggcggtgaca ccaatatgat
    57361 aatcattgaa ggcatcccac aacccatcat gcaccagact gtcaaccaac tggctattgc
    57421 caagctgttc accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc atgttttcct
    57481 ggccacctgc gatgacaata tcagcctcgc cacactgtat cgcctgagta gccagatgca
    57541 gtgctttaag accggaaccg caaacgtcat taatcgtgat tgcagaaacg ctgttaggaa
    57601 gaccaccttt aatagccgat tgccttgccg gattctgccc tgcccctgca gtcaacacct
    57661 gaccaagaat tacttcatcc accgcatatg caggaacgcc ggtacgttct attaacgctt
    57721 tcacgaccag actaccaagt tccacggcgg aatgacccgc taacgcacca cgaaagcagc
    57781 cgataggtgt ccgtaacgcc ccgacaatca caacgtcttt catcacaacc tcgcaccaat
    57841 taacagtgca atagtaaatg attgttaatt acagttatct taattgttta aaaaaagtga
    57901 tttttatcac aaaggaaata tgcctgagca gtagtcagag tcataactgg cacgtaaggt
    57961 ttgcaaccac taacccacca atagatgggt agatagggcg ttaatctccc atacttaact
    58021 tggtttatgg taaattgccc tccattttgt ttaatttgta gatgatacgt tcagataatg
    58081 tctaatattt ggtctaaaga agaaactctg tgaagtttcg cgctctatgg cacagccgtt
    58141 gatgcaggca cgctattcct tcctattcag ttaggttcgg caggggctgt ggtcctgttt
    58201 attactgctc tggtcgcctg gcctttaaca tattggccac ataaagcctt atgccagttc
    58261 atcctctcat caaaaacatc agcaggtgaa gggataacgg gcgcggtaac acactactat
    58321 ggcaagaaga ttggtaatct gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg
    58381 ttgatatatg cagtggcaat taccaactca cttacggaac agctggcaaa gcatatggtt
    58441 attgatcttc gcatccgtat gttggtgagt ctgggtgttg tattaattct gaatctcatt
    58501 tttctgatgg gacgtcatgc cactattcgg gtaatgggat ttttggtatt cccattgatt
    58561 gcctatttct tatttctttc catttacctt gtcggtagtt ggcaacctga tctattaaca
    58621 acccaggtag agttcaatca gaataccctt caccagatat ggatatcgat tcccgtgatg
    58681 gttttcgcct ttagccatac gcccattatt tctacgtttg ccatagacag acgtgaaaaa
    58741 tatggcgaac acgctatgga taaatgcaaa aaaattatga aagtcgctta tctcatcatc
    58801 tgcataagtg tactgttctt tgtctttagc tgcctgcttt ctattccatc ttcgtatatt
    58861 gaagcggcta aagaagaagg ggtcaccatt ttatcggcgc tttctatgct gccgaacgcc
    58921 ccagcatggt tgtcaatttc cgggattatt gtcgcagtag ttgcgatgtc gaaatcattc
    58981 ctgggtacgt actttggcgt tattgaaggt gccacagagg tcgtcaaaac aacactacag
    59041 caggttggtg taaagaaaag tcgtgcattt aaccacgcac tatcaattat gttggtatcg
    59101 ctgattacct tcattgtttg ttgcattaac ccgaacgcga tttcgatgat ttacgcgatc
    59161 agcggcccgc tcattgccat gatacttttc atcatgccta cgctgtcaac gtatctcatc
    59221 ccggcgctta aaccctggcg ttccatcgga aatctgatta cgctgatcgt gggtatcctg
    59281 tgcgtatcgg taatgttctt tagctaatat ccgttgtgga tggttcaaaa ttgctacaat
    59341 tttgaaccat tccatttttc ataatattca tttgttatta ttccgcccta tcacttctct
    59401 cgtcatatcc tagaattatc agttagttaa acacacaaag agatctgaac atcttaaata
    59461 agactattta agatgcataa cttagattcg caagatatct tctggtcatt caaaaacaat
    59521 ttgcgatata gatattctca taccatgaga tagttatctg gaccacgttc gaccatgcaa
    59581 gattaaatca gtgaaacata atcatattta ataacgagaa atgcattttt aaatatctta
    59641 atcagaatta tagcaaatac agattaatcg caatttttcc cagcaaaatt acaaaatcaa
    59701 ggtgtattga catcatcaat caccaatgaa attatgcaat tatatacgga tagggaggtt
    59761 cttaacatgg ggctttgtag tcgttataaa agtcttacat gcaatagttg ctcaatgcat
    59821 tgccaaataa tgccagaaga gtcaccgcgt ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAZSNG010000012        59835 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZSNG010000012 DAZSNG010000000
VERSION     DAZSNG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59835
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.012"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            123..1811
                     /locus_tag="ACHW4Z_000765"
     CDS             123..1811
                     /locus_tag="ACHW4Z_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR8880526.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            1808..2722
                     /locus_tag="ACHW4Z_000766"
     CDS             1808..2722
                     /locus_tag="ACHW4Z_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8880527.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2755..3003
                     /locus_tag="ACHW4Z_000767"
     CDS             2755..3003
                     /locus_tag="ACHW4Z_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8880528.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3003..4174
                     /locus_tag="ACHW4Z_000768"
                     /pseudo
     CDS             3003..4174
                     /locus_tag="ACHW4Z_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4210..5289)
                     /gene="lptG"
                     /locus_tag="ACHW4Z_000769"
     CDS             complement(4210..5289)
                     /gene="lptG"
                     /locus_tag="ACHW4Z_000769"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8880529.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5286..6356)
                     /gene="lptF"
                     /locus_tag="ACHW4Z_000770"
     CDS             complement(5286..6356)
                     /gene="lptF"
                     /locus_tag="ACHW4Z_000770"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8880530.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6387..6947)
                     /locus_tag="ACHW4Z_000771"
     CDS             complement(6387..6947)
                     /locus_tag="ACHW4Z_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8880531.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6959..7783)
                     /locus_tag="ACHW4Z_000772"
     CDS             complement(6959..7783)
                     /locus_tag="ACHW4Z_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880532.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7783..9132)
                     /locus_tag="ACHW4Z_000773"
     CDS             complement(7783..9132)
                     /locus_tag="ACHW4Z_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR8880533.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9178..9936)
                     /locus_tag="ACHW4Z_000774"
     CDS             complement(9178..9936)
                     /locus_tag="ACHW4Z_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880534.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(9968..10681)
                     /locus_tag="ACHW4Z_000775"
     CDS             complement(9968..10681)
                     /locus_tag="ACHW4Z_000775"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8880535.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10855..11547
                     /locus_tag="ACHW4Z_000776"
     CDS             10855..11547
                     /locus_tag="ACHW4Z_000776"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8880536.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11596..13095)
                     /gene="pitB"
                     /locus_tag="ACHW4Z_000777"
     CDS             complement(11596..13095)
                     /gene="pitB"
                     /locus_tag="ACHW4Z_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR8880537.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13387..15246)
                     /gene="gss"
                     /locus_tag="ACHW4Z_000778"
     CDS             complement(13387..15246)
                     /gene="gss"
                     /locus_tag="ACHW4Z_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR8880538.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15451..16317
                     /gene="yghU"
                     /locus_tag="ACHW4Z_000779"
     CDS             15451..16317
                     /gene="yghU"
                     /locus_tag="ACHW4Z_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR8880539.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16346..16427)
                     /locus_tag="ACHW4Z_000780"
                     /pseudo
     CDS             complement(16346..16427)
                     /locus_tag="ACHW4Z_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16440..16688)
                     /gene="hybG"
                     /locus_tag="ACHW4Z_000781"
     CDS             complement(16440..16688)
                     /gene="hybG"
                     /locus_tag="ACHW4Z_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR8880540.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16822..17163)
                     /gene="hypA"
                     /locus_tag="ACHW4Z_000782"
     CDS             complement(16822..17163)
                     /gene="hypA"
                     /locus_tag="ACHW4Z_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8880541.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17156..17644)
                     /gene="hybE"
                     /locus_tag="ACHW4Z_000783"
     CDS             complement(17156..17644)
                     /gene="hybE"
                     /locus_tag="ACHW4Z_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR8880542.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17637..18131)
                     /locus_tag="ACHW4Z_000784"
     CDS             complement(17637..18131)
                     /locus_tag="ACHW4Z_000784"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR8880543.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18131..19834)
                     /gene="hybC"
                     /locus_tag="ACHW4Z_000785"
     CDS             complement(18131..19834)
                     /gene="hybC"
                     /locus_tag="ACHW4Z_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR8880544.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19831..21009)
                     /gene="hybB"
                     /locus_tag="ACHW4Z_000786"
     CDS             complement(19831..21009)
                     /gene="hybB"
                     /locus_tag="ACHW4Z_000786"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR8880545.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20999..21985)
                     /gene="hybA"
                     /locus_tag="ACHW4Z_000787"
     CDS             complement(20999..21985)
                     /gene="hybA"
                     /locus_tag="ACHW4Z_000787"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR8880546.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21988..23106)
                     /gene="hybO"
                     /locus_tag="ACHW4Z_000788"
     CDS             complement(21988..23106)
                     /gene="hybO"
                     /locus_tag="ACHW4Z_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR8880547.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23295..23582)
                     /gene="yghW"
                     /locus_tag="ACHW4Z_000789"
     CDS             complement(23295..23582)
                     /gene="yghW"
                     /locus_tag="ACHW4Z_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR8880548.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23701..24588)
                     /gene="yghX"
                     /locus_tag="ACHW4Z_000790"
     CDS             complement(23701..24588)
                     /gene="yghX"
                     /locus_tag="ACHW4Z_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR8880549.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24745..25785
                     /gene="gpr"
                     /locus_tag="ACHW4Z_000791"
     CDS             24745..25785
                     /gene="gpr"
                     /locus_tag="ACHW4Z_000791"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR8880550.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25825..26319)
                     /locus_tag="ACHW4Z_000792"
     CDS             complement(25825..26319)
                     /locus_tag="ACHW4Z_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR8880551.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26510..27394
                     /gene="yghA"
                     /locus_tag="ACHW4Z_000793"
     CDS             26510..27394
                     /gene="yghA"
                     /locus_tag="ACHW4Z_000793"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8880552.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27666..28091)
                     /gene="exbD"
                     /locus_tag="ACHW4Z_000794"
     CDS             complement(27666..28091)
                     /gene="exbD"
                     /locus_tag="ACHW4Z_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR8880553.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28098..28832)
                     /gene="exbB"
                     /locus_tag="ACHW4Z_000795"
     CDS             complement(28098..28832)
                     /gene="exbB"
                     /locus_tag="ACHW4Z_000795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8880554.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29084..30271
                     /gene="metC"
                     /locus_tag="ACHW4Z_000796"
     CDS             29084..30271
                     /gene="metC"
                     /locus_tag="ACHW4Z_000796"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR8880555.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30411..31070
                     /gene="yghB"
                     /locus_tag="ACHW4Z_000797"
     CDS             30411..31070
                     /gene="yghB"
                     /locus_tag="ACHW4Z_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR8880556.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31110..32009)
                     /gene="yqhC"
                     /locus_tag="ACHW4Z_000798"
     CDS             complement(31110..32009)
                     /gene="yqhC"
                     /locus_tag="ACHW4Z_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR8880557.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32203..33366
                     /gene="yqhD"
                     /locus_tag="ACHW4Z_000799"
     CDS             32203..33366
                     /gene="yqhD"
                     /locus_tag="ACHW4Z_000799"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR8880558.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33471..34298
                     /gene="dkgA"
                     /locus_tag="ACHW4Z_000800"
     CDS             33471..34298
                     /gene="dkgA"
                     /locus_tag="ACHW4Z_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR8880559.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34498..35424
                     /locus_tag="ACHW4Z_000801"
     CDS             34498..35424
                     /locus_tag="ACHW4Z_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR8880560.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35475..35732
                     /gene="yqhH"
                     /locus_tag="ACHW4Z_000802"
     CDS             35475..35732
                     /gene="yqhH"
                     /locus_tag="ACHW4Z_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR8880561.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35775..37994)
                     /locus_tag="ACHW4Z_000803"
     CDS             complement(35775..37994)
                     /locus_tag="ACHW4Z_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR8880562.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38105..39517)
                     /gene="ftsP"
                     /locus_tag="ACHW4Z_000804"
     CDS             complement(38105..39517)
                     /gene="ftsP"
                     /locus_tag="ACHW4Z_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR8880563.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39592..40329)
                     /gene="plsC"
                     /locus_tag="ACHW4Z_000805"
                     /gene_synonym="parF"
     CDS             complement(39592..40329)
                     /gene="plsC"
                     /locus_tag="ACHW4Z_000805"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR8880564.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40563..42821)
                     /gene="parC"
                     /locus_tag="ACHW4Z_000806"
     CDS             complement(40563..42821)
                     /gene="parC"
                     /locus_tag="ACHW4Z_000806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR8880565.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43367..43849)
                     /locus_tag="ACHW4Z_000807"
     CDS             complement(43367..43849)
                     /locus_tag="ACHW4Z_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR8880566.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43902..44294)
                     /gene="ygiW"
                     /locus_tag="ACHW4Z_000808"
     CDS             complement(43902..44294)
                     /gene="ygiW"
                     /locus_tag="ACHW4Z_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR8880567.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44446..45105
                     /gene="qseB"
                     /locus_tag="ACHW4Z_000809"
     CDS             44446..45105
                     /gene="qseB"
                     /locus_tag="ACHW4Z_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR8880568.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45102..46376
                     /gene="qseC"
                     /locus_tag="ACHW4Z_000810"
     CDS             45102..46376
                     /gene="qseC"
                     /locus_tag="ACHW4Z_000810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR8880569.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(46422..46754)
                     /locus_tag="ACHW4Z_000811"
     CDS             complement(46422..46754)
                     /locus_tag="ACHW4Z_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR8880570.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            47073..47654
                     /gene="mdaB"
                     /locus_tag="ACHW4Z_000812"
     CDS             47073..47654
                     /gene="mdaB"
                     /locus_tag="ACHW4Z_000812"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR8880571.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            47685..47999
                     /locus_tag="ACHW4Z_000813"
     CDS             47685..47999
                     /locus_tag="ACHW4Z_000813"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR8880572.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(48047..49939)
                     /gene="parE"
                     /locus_tag="ACHW4Z_000814"
     CDS             complement(48047..49939)
                     /gene="parE"
                     /locus_tag="ACHW4Z_000814"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR8880573.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(49968..50549)
                     /gene="yqiA"
                     /locus_tag="ACHW4Z_000815"
     CDS             complement(49968..50549)
                     /gene="yqiA"
                     /locus_tag="ACHW4Z_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR8880574.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(50549..51376)
                     /gene="cpdA"
                     /locus_tag="ACHW4Z_000816"
     CDS             complement(50549..51376)
                     /gene="cpdA"
                     /locus_tag="ACHW4Z_000816"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR8880575.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(51401..51823)
                     /locus_tag="ACHW4Z_000817"
     CDS             complement(51401..51823)
                     /locus_tag="ACHW4Z_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR8880576.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(51824..52453)
                     /gene="nudF"
                     /locus_tag="ACHW4Z_000818"
     CDS             complement(51824..52453)
                     /gene="nudF"
                     /locus_tag="ACHW4Z_000818"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR8880577.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            52658..54139
                     /gene="tolC"
                     /locus_tag="ACHW4Z_000819"
     CDS             52658..54139
                     /gene="tolC"
                     /locus_tag="ACHW4Z_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR8880578.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            54287..54958
                     /locus_tag="ACHW4Z_000820"
     CDS             54287..54958
                     /locus_tag="ACHW4Z_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR8880579.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            54964..56124
                     /locus_tag="ACHW4Z_000821"
     CDS             54964..56124
                     /locus_tag="ACHW4Z_000821"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8880580.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(56162..56950)
                     /gene="ygiD"
                     /locus_tag="ACHW4Z_000822"
     CDS             complement(56162..56950)
                     /gene="ygiD"
                     /locus_tag="ACHW4Z_000822"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR8880581.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            57093..57866
                     /gene="zupT"
                     /locus_tag="ACHW4Z_000823"
     CDS             57093..57866
                     /gene="zupT"
                     /locus_tag="ACHW4Z_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR8880582.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(57924..58094)
                     /gene="yqiD"
                     /locus_tag="ACHW4Z_000824"
     CDS             complement(57924..58094)
                     /gene="yqiD"
                     /locus_tag="ACHW4Z_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR8880583.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(58355..59008)
                     /gene="ribB"
                     /locus_tag="ACHW4Z_000825"
     CDS             complement(58355..59008)
                     /gene="ribB"
                     /locus_tag="ACHW4Z_000825"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR8880584.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(59113..59261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            59383..59673
                     /gene="ubiK"
                     /locus_tag="ACHW4Z_000826"
     CDS             59383..59673
                     /gene="ubiK"
                     /locus_tag="ACHW4Z_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR8880585.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        14984 a        15865 c        14829 g        14157 t
ORIGIN      
        1 aaaatactat ctcatttatc ttttatgcct atataatcca ccgcattatt gtcattttat
       61 ttttgatatc caattttttc gtggtgtata tgtctaataa aatctttacg cattccctac
      121 ctatgcgcta tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc gctctgagta
      181 gagccggaat gaatttttat gacagacgtt gccaacttga agatcaactg gaatatcaga
      241 cattaaaaac gcgtgccgaa gctggtgcga agcggttgtt atcgctgaac ctgaaaaaag
      301 gcgaacgcgt ggcgctgatt gccgaaacga gtagcgggtt tgtagaggct ttttttgcct
      361 gtcagtatgc tggcttagtc gccgtcccgt tggcgattcc aatgggcgtc ggtcagcggg
      421 attcctggag cgccaaactc cagggtttac tggcaagttg ccagcccgca gccattatca
      481 ctggtgatga gtggttgcca ctggtcaatg ccgcgacgca taacaacccc gaattacatg
      541 ttttaagcca cgcttggttt aaggcattac cggaagccga tgttgcgctc cagcgtccag
      601 ttccgaacga tatcgcctac ctccagtaca cctccggcag cacccgtttt ccccgtggcg
      661 tcattatcac ccatcgcgaa gtgatggcta atctacgtgc tataagccac gatggcatta
      721 aattacgccc tagcgaccgc tgcgtctcct ggcagccttt ctaccatgat atgggactgg
      781 tcggctttct cctgcccccc gtcgccacgc aactttcagt agattatttg cgcactcagg
      841 attttgccat gcgtcctttg caatggctta aattgatcag taaaaatcgc ggcaccgttt
      901 ccgttgcgcc gccgtttggc tatgaattgt gccaacgccg cgtgaatgaa aaagatctcg
      961 ctgaactgga tctttcctgc tggcgcgtcg ctggtattgg tgctgagccg atctccgcag
     1021 aacaactcca tcaattcgct gaatgtttcc gtcaggttaa ctttgacaat aaaactttca
     1081 tgccgtgcta cggactggca gaaaatgcgc tggctgtcag cttctctgat gaagcctccg
     1141 gggttgtggt taacgaagtg gatcgcgaca tcctcgaata tcagggtaaa gccgtcgcgc
     1201 cgggtgcaga gacacgcgcc gtatcgactt tcgtcaactg cggcaaagcg ttgccggaac
     1261 atggtattga aatccgcaat gaagcaggta tgccggtcgc ggaacgtgtg gtaggccata
     1321 tttgcatctc cggtcccagt ctgatgagcg gttactttgg cgaccaggtt tcgcaagacg
     1381 agattgccgc gacgggctgg ttagacaccg gcgacctcgg ttatctgctg gacggttatc
     1441 tgtatgtcac cggacgcatt aaagatctga ttattattcg tggccgtaat atctggccgc
     1501 aggatattga atatatagcg gaacaggaac cggaaattca ttctggcgat gcgattgctt
     1561 ttgttaccgc ccaggaaaaa atcattttgc agatccagtg tcggatcagc gacgaagaac
     1621 gtcgcgggca gcttatccac gcgctggcgg cacggatcca aagcgaattt ggcgtgaccg
     1681 cggctatcga tctgttgccg ccccacagta ttccccgaac gtcctccggc aagcctgccc
     1741 gtgcggaagc gaaaaaacgt tatcagaagg cttatgctgc cagtcttaat gtgcaggaat
     1801 ccctggcata aatcaaactg tcgcggtgac gggcgctacc gggtttatcg gtaaatatat
     1861 tatcgataac ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta ctgcccgcgc
     1921 tcacgtcaat gataatctca cctgggtgcg cggttcgctg gaagatactc attcacttag
     1981 cgagctggtt gccggagcca gcgtagtggt ccattgcgcc gggcaagtgc gcgggcacaa
     2041 agaagagatt ttcacccgct gtaacgttga cggcagcctg cgcctgatgc aagcagcaaa
     2101 agagagcggc ttttgccaac gttttctgtt tatctcttcg ctggcggcgc gccatcccga
     2161 gctctcctgg tatgcaaaat ccaaatacgt cgccgagcaa cgactggcag caatggcgga
     2221 cgaaattacg ctgggcgttt ttcgcccgac agccgtgtat ggtcccggcg ataaagagtt
     2281 aaaaccgctg tttgactgga tgctacgcgg cctgctgcca cgacttggtg cgccagatac
     2341 acaactctct tttcttcacg tcaccgattt tgcgcaagca gtgggtcagt ggttaagcgc
     2401 cgaaactgta cagacgcaaa cctatgaatt atgcgatggc gtcgccggtg gctatgactg
     2461 gcaacgcgta cgacagcttg ccgccaaagc ccgttgtggt tccgtgcgaa tggttgacat
     2521 tcctctgccg gtactcacag gccttgcgga tatcagtacc gcgttgagtc gcctggcagg
     2581 taaagaacct atgctgaccc gctcgaaaat tcgtgaatta acccacgccg actggtcggc
     2641 aagtaataac cgtatttctg aagatatcaa ttggtttccc gggattagcc tggaacacgc
     2701 attacgcaac gggctatttt gattgtcttg ttttaaaggt gtgttaacgt ttctatggta
     2761 aatcgtgaaa tagtaatgga ttacatcctt tcctgtttac aggatttggt cgaaaacggt
     2821 gtggaaatta agcccgacag cgatctcgtc aacgatcttg gcctggagtc gatcaaagtg
     2881 atggatctct taatgatgct ggaagacaga ttcgatattt ccattcctat taatattctg
     2941 ctggatgtca aaacccctgc gcaattaatg gaaactctac ttccatggct ggagaataaa
     3001 taatggggct atacgataaa tatgcgcgcc ttgctggcga gcgtctgcaa tttagtgaca
     3061 acgggttaac accgtttggc acctgtattg atgaagtgta ttccgccacc gaaggccgga
     3121 tcggcaataa aaaagtgatt ctggccggga ccaataatta tctcggttta acctttaatc
     3181 atgatgccat cagcgaaggt caggccgcgt tagcggccca aggcaccggc accaccggtt
     3241 ctcgtatggc aaacggcagt tatgcgccgc acctggcgtt agagaaagag attgccgagt
     3301 ttttcaatcg ccctaccgct atcgtttttc aaccggttat accgctaatt taggcgtcat
     3361 cagcgcactt gccgaccata acgccgtggt gttactggat gccgacagcc acgccagtat
     3421 ttatgacgtc tgttctctcg gtggcgcgga aattattcgt ttccgccata acgatgctaa
     3481 agatctggaa cggcgcatgg ttcgcctcgg tgaacgggcg aaagaggcga ttatcatcgt
     3541 tgagggcatc tacagcatgc tcggtgacgt tgcgccgctg gcggaaattg ttgatatcaa
     3601 acgtcgtctc ggtggttatt tgattgtcga tgaagcgcat tcgtttggcg tgttaggcgc
     3661 aacggggcgc ggactggctg aagctgtcgg cgttgaagat gatgtggata ttatcgttgg
     3721 tactttcagt aaaagtctgg cctcgattgg cggttttgct gtcgtttcgg aagcgatgga
     3781 agtgctgcgc tacggcagcc gcccgtatat ttttaccgcc tctccttctc cgtcctgcat
     3841 tgcgaccgtg cgctcgtcgt taagaactat tgccacacaa ccagagctgc ggcaaaagct
     3901 gatggataac gccaaccatc tgtatgacgg tctgcaaaaa ctgggttatg agttaagttc
     3961 acatattagc ccggtagtgc cagtaataat cggttcgaaa gaggaaggtt tacgcatctg
     4021 gcgcgagctt atctcgccgg gcgtgtatgt gaatcttatc ctgccgcctg ccgcgcctgc
     4081 aggcatcacg ctgctgcgct gtagcgtaaa tgctgcccac agtcgtgagc aaatcgatgc
     4141 catcattcag gcatttgcta ctctgaagca gtaatgtgag cccgccgtgg tgtttgcgcc
     4201 acggcgggtt taatgttgtc tgtcatacca atacaccagg ctcaacgcca ccaacacaaa
     4261 cgctaccggt aaaccgagcg cggtaacagg tgcgcttaac gcaaatagca atcccagatt
     4321 aaccatgatt tgatagctga tccaggtgag taagcccacg atgacgccta cagcgagacg
     4381 gctacccatc ccaggcgaac gcggggcact aaaggtaaac ggcacagcca gcaaaatcat
     4441 cgccagggtc aggatgggtt gccccagttt ttcccacagt gcaaggtggt attcgctgct
     4501 gggttgcccg gtttcctgca aataatggat gtaatgatta agctgacgga cagaaaaagt
     4561 attccccggc attgataact cttcaagatt catactggtg aaggccgatt gccaggcaag
     4621 attatctaat gtttccagcg tttctttgcc attcagccat tttttatgat tcacaccatg
     4681 caggacccac gttttgtcgt cttcaatggt ggcactgcgt gcataaatgt aggattcaag
     4741 cgagagatcg tcgcgataat gaaatatctc cacgcccacc ggctggccct gctcgttcag
     4801 cgatttcacc gtcacaaatt cattaccacg cctggcccac agcatattgc cggtaatgtc
     4861 atcgtcctcc cccaacgcgg tggcggtcga tttgatttgc aacgcctgtt gctgcaatgg
     4921 cgacgccacc cactcatcta tcgcgcctaa cgaaacagtc cacaatatcc cggcaaccag
     4981 cgccaccagt gcaatacgga agatagaaaa ccccgtgctg cgaatggcgg taagctcact
     5041 gtttttcgat aactgcccca gtccaacaat accacccaat aacgcgataa acggcgaaag
     5101 ttcgaccagt gtgcgcggta aggtcattag caccaccagc accgcctgag tccagcgata
     5161 accgcccggg ctgacaccat ccagttcgtt aatcaggttg aaggtggtaa aaagcggcag
     5221 caatagccct gcagcggcgg caaaaccgag aaagagatga cggattaaat agcgactgaa
     5281 aacattcatc gggaaaattt ctgcagcaaa gagaagtcac gtgcgaccag tatcagcagg
     5341 cccattagca tgagtccggg cactaaccac aaaccaggaa tgaggggaat cgcgccgtta
     5401 gccaccagtg tacggcagac gtcgccgcca taaaaaatgg caataaacag caacgtcagt
     5461 ggcaataacg ttgaaaatcg cccttgccgc ggttttaccc gacttaatga aatggccagc
     5521 agcgccatca acaatgcggt caggccacgg ctttgtcgcc attgcagttc ggcatgatcg
     5581 gcaggaaagg cggagcgcgc cagctccgtg accgatttcg ctttgcgttt gacgttcggg
     5641 ctttgatcca gcggattcag atgtaattgc agattacggt agatctgttc gttgtcgtca
     5701 cgcccctgat gatcgagaag ataggcagtg ccgttgtgca acataacggt cggtttctcg
     5761 ggtgatgggt caaccacatc aaccgaaccg gcgcggaaaa tacgggttcg attggcagta
     5821 gaagtataaa tcagcgcgtc agtcaggcga ttattatcct gatcaaccgt ctgcgaaagg
     5881 atcattcgtc cgttatcgtt agtgttaaat ttctttgccc gcaactggcg aacatccagc
     5941 tccgactgcg actgctgctc cagttgataa atctgcgcat aagcccacgg acgaccatac
     6001 atcgacagaa gggtgacaaa aatacttagc ggtatcgcca gataaagaac ggctttgtac
     6061 aagcgtcccg gactgccccc cgccgcagag atagcggtaa tttccgagtc ggtatacatc
     6121 tgccctagcg tcacgccaac tgacacatac agaccaacag gtaacaacat ctccagtgca
     6181 atcagtactt tgtagaaaac gatatccagc acaacatcaa gagctaatgt cccatttgcc
     6241 gcttcggtca gataacgctg tgcggagtag ctggcgaaaa taaagatcag aaaaccgacg
     6301 ataatcagca ccagacggcg cgtaccacgc atgatgtagt gttcaaccag cttcatctca
     6361 ctaacgccct caatcctttg agttttttat tcattgatca gtttctgcgc caacgcaatg
     6421 acatgatcta cctcttccgg cgtcaggctg ccgtcctttg cccactgaat ttgcccggct
     6481 ttattcagca ccagaatggt tgaactctgc tccggcaagc gccaggcaac gcgtccctgt
     6541 ccattgccgt caataacgaa ttgcgcccac ggatagcggc gtttattttt ttcgattttg
     6601 ccaacaacaa aatagccagt accaaagatg gcatcatcgg tgttcacaat ggtggtgggc
     6661 tgaaaacgat cctgcggaaa gtttgccgct tcaaccgctt taattaacag tgagttcttt
     6721 ttcttcgccg acttacgccc ggcgatatac tgaataatgc ggacttttcc cgctaattgt
     6781 gtgctgttcc acttttggta gtgaatttcg tcgtcgctat tcagaagcag ctcgccgcct
     6841 tcctggatat aaacaggggt aaccgttttg ccagtgacga aattgtgtgc ctgcacaccc
     6901 ggggctaaga gcatgataat caacgcaata atcaaacgag atgacatcat tgctccggct
     6961 atagcggaaa acgcgaaatc agcagctcta agcgctgaca aatatcgcta tccagcgcca
     7021 acggtgcata atacgactgg cggttttcca tatcatccgc caccatgtcg tcgtgtttat
     7081 ctcccatcag gccattaatg gcggcgagga aacgggaaat ctgtaacgcc tctttatttt
     7141 caggatcaag cgatcttaag gtgtcgtaat aagaaacctg ctggctgatg atttttcgca
     7201 catcatgaaa ttccctgccg gttagtcgcg gtttggcaat aatctctttc agggtatgca
     7261 tttgcttttg ccgataggca ataaaactgc ttacgctttc taactggcta tggcgtaatg
     7321 aatagttcac caacgaccag accggagagc ttaaataaat ccgtaacagg ttgccgtaat
     7381 acgacacaat atttttctta ccgttacgaa agccgtcctg aaaacgcccc agaaaaacgg
     7441 tcgttttgcc gaataaaaat ccggcctgat gcttctttaa atacagccgt tgcgcaaaac
     7501 gcaggtgctt gtagcgcgct cgcatgtatt taaattgttt gcgctcatca gcagtcagct
     7561 catcgccttt ggcttgtttg cgaactaaac gcagtaattc ctgacgatca acgccattga
     7621 gccagaattg caggcataac gcgtaattat catgaatgat ttcggtggta caacttagtg
     7681 caatagtcgg cggcaaacta acctgtgcat caacgacatc atcttcttca atcgcaacaa
     7741 atagacgctc gctcgcctca agcgggaaat attttctcat catcaggatt tctctccggc
     7801 tgcgttataa agcaaggaat ggcgctgaaa aaatgccttt aaggttggaa ttaacgagag
     7861 cgccacgtcc agacattgcc caccgaagta agcaaacgct gctcccagca caccaaacag
     7921 atgacagaac atgaacagca atacgatgta gccgattgag gcgatggttt gcgccacgag
     7981 aaaagcgcgc tgttttcccg ccatcaatag caatgactcc tgcgggaagc ccagcatcga
     8041 aatcacaatt gcgcccagca tcacctgaat caggtcatac gcttcgagat acttaacgcc
     8101 aaacaccagc gaaatgagcg gcttgccgac gatcaacacc gcgagcgcca ccagaatacc
     8161 gattccgccc gccagtaaac cggacttcac acctaacaac cacggtctgg tggtgcgcgg
     8221 atctaaacgc atcacctctg ggtaaaaact tttacccagc aaacccgctg gcgttccggc
     8281 ggcgtcgaag aatgtcatgg cgattttaaa taacccggcg gcagcgggtc ctaacacgat
     8341 ccccaccaac actgtgctgc acgagttacg cgccgaccag atggagtggg caatgttggt
     8401 tgaccagaca aaactccacg cgcctttaat gtgtcgggca gactcaaaca gattcaattt
     8461 gaaggcgtta tggatatttc tacggcgtaa ttcgcgcgcg gcaaaccacc agtacatggt
     8521 gccgccaacc agattcgaca cgtaccaagc aataacaaaa cccacaaaac caaagtcaaa
     8581 ataccaggct gcgacgctcc ccgctgcgcg cagaaaaggt ttcgtcgcct gctgtacagc
     8641 aatcaaatca aagcgatcta ccgcacgcag aatgccggtc ggcgtggagg aagccattga
     8701 aggaatgagc gtgcaataca gcgccgccag ccaaaagctt tggtcatcca gacctaatga
     8761 atgggaaagg aatggcagta aggcaatgcc gccgacaatc gccaccgcgc cgctgacgat
     8821 atccagcgag aaggaaaatg agacgacatt gcgaaattgc tgcggattat tgttggtcaa
     8881 ggctggcgtt ccgtactgaa ccaccagctg ccatgtctga aacttaataa aatcgctgat
     8941 cgacttggcg tacgattgca caatcaccag tacaccaaac atggcgggcg tcatcccttt
     9001 accggcacac gagagcgcca acagccccag caaggcgctc tcgacattac tggagcctaa
     9061 ccaggcgcta ttgcgaataa tggtgcgaaa cgcgccatct gcaaaccaat gtttgatgtt
     9121 aaaacccgcc aattcagcct gaccttatcg ttgatagtaa aaaatatccc gccagcctta
     9181 agttaaactt cggcggtcag aaacgatggc aaccagagaa accgccttct gtacctgttc
     9241 cagcacttcg ctgtagggcg ctctggaatc aatctcaaga atttttgtgc cgttatagcc
     9301 aatcgtcgac atgacgccga ttttgtcctg cagctcggca tactcatggt caggcttgcg
     9361 ggagatggca gtatcaatat caatccccaa acgaatgatt aattcaggac ggtattgcgc
     9421 cattttttgg tataaactcc gttcgcgctg cgccagaaac atgctgattt tcccggtcgc
     9481 acgttcgacg ccgatccccg gtccgtcata ataaaagccc gaaatttcag cctgcgggaa
     9541 gcgatcgctg accaccagaa cgccactttg cgccagtcgc tgaaccttgc gtagattcgc
     9601 cattcttcgc agcgagaagc agtacataat caccgccgcc catagcgccg gagatttggt
     9661 tttcatgctt tgggttttcg aggatttggc cgccaatcgc cgttccagcc agacgccaac
     9721 caacggcaat cgtttgattt tgtcgccgtc ttcgccggag agcagcccca gatagcggcg
     9781 ctcggtttgc cagtgttgtt gcagcgattt caccaggtcg gtggtcagcg tggatttacc
     9841 ggtgccatca cacccgacca ccgcaatcag ccccggaatg tagttgggct gcggcgcggt
     9901 tgtactatta acagtttgtg tttgtagtgc atccattcgt taaatcatcc cctatccatt
     9961 ttggtgatca ggaaagcgcg gcgtgaattg cgcgtagtga cgcttgcata atttcatcgg
    10021 ctggttgccg accatccagt tcaaggatct tggcgccatt gaatgtcagt tgcggcgtaa
    10081 cggcgatttt ttcctgtaac gctgccagtt ggtggtcagg tttacgcgca aacgcggttt
    10141 gttcatcaat gccaagacga atcaacaata cgggtaaata agacgccatc cattggtaca
    10201 gcttcaactc gcgctgcctt aacattttta tccaaccgtt accgcccgtg gtttttgcca
    10261 attgcgggcc atcaaagcga aaacccggca cttcaacctg cgggtagcgg tcggtgatga
    10321 gcagatagcc ttgctggctt ttacacaaca ttttgcgaaa cttgtacgcc cgccagcagg
    10381 aaagcagata gataaccagc gcagtaatat tgccaggcgg agttaagggc ttttcgtgta
    10441 catgtgccgc tttacttcgc agataacacc cacaaggtgc gccaataaca gggagctgtg
    10501 aaatccattc gccaattcgc ccggacgatt gcccgagata aatgtgttct gttggcattc
    10561 ttgctgccag ttcatttacc aggcttgccg tgagggtcga tttacctgaa ccatcacatc
    10621 ctacaatggc aataacacgc actggcgtgg aattaattat tgacatatta cgttgattca
    10681 cgaaaaaccc ggcagtaaat aatgtattga atattacgtg gtccgtgctc agaatatccg
    10741 ttcaacctta ttgtgacaat gactaattgt gacattgatc tgaattacgc tttcataaaa
    10801 acatattaac caaataaata tttttaatgg atatttaaat taaaggatat attcatgcag
    10861 tcaataacac ctccattaat tgccgttatt ggtagcgatg cttcaggcaa gtcaacggtg
    10921 tgtgaacatc ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga
    10981 aaacaggccg gaaatgtcgg tcgtgcagtg acaaaattac ctttgatggg aaaatcctta
    11041 cataaaacaa ttgaacgaaa tcaggtgaaa acagcaaaaa aattgcctgg accagttccg
    11101 gcgctggtaa ttacagcgtt tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc
    11161 tgtcgtcgtc gcgggttaat tgttctaacc gaccgttatc ctcaggacca aattcctggc
    11221 gcttacgatg gtacggtgtt cccacctaac gttgaaggtg gtcgttttgt ctcatggctg
    11281 gcaagccagg aacgtaaagc gtttcactgg atggcgagcc ataagcctga tctggtcatc
    11341 aaactcaatg ttgaccttga agttgcctgt gcacgtaaac ccgaccataa acgggaatcg
    11401 ctggcgagga agattgccat aacgccacag ttaacctttg gtggtgcaca actggttgat
    11461 atcgatgcca atcagccact ggaacaggtg ttggttgatg cagaaaaagc gattacggat
    11521 tttatgaccg cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg
    11581 gcattttcag gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa
    11641 aattgccgcc ggtaaagtaa acacccacgc catcaggatg ctggttaccg ttttacgctg
    11701 taacccaccg ccgtccacca ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt
    11761 ggagacaggc atcccaatat aactggcaag gccgatagac actgccgccg tcatttgtgc
    11821 cgccatgcct tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc
    11881 tacacgacgc cagccaatca tggtgccaat gccgagcgcc agtgctaccg ccatgatgat
    11941 ccacaccgga gcgtactcaa tggtgcttaa catatcgctg cgcagttttt tcagcaggtt
    12001 ctggtcttct ttactgacgc ctggcagttt cgccagcttc gcggaggtat cggagatgca
    12061 cagcataatg cggcgcagct ggctgcgctg actcacgctt aacggctcgt aactttccat
    12121 attgcctggc agcatcgttt taacgcgcgc aatcgcatca aaggtatttg ccggatgaca
    12181 gtgaaactct gttacttgcg tgccatcagt cgatgctgca ggcaatggag gttccatcgc
    12241 aatcaacttc tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt tggtaacggc
    12301 atcgcgggta cgggtaattt catagccgga cgcattcata ttgacgacga agccagcagg
    12361 ggcaatcccc accagtacca gcattaccag gccgatccct ttctgcccgt cgttcgcgcc
    12421 gtgcgaaaac gccacgcccg cagcggaaac aatcagcgca atacgcgtcc agaatggcgg
    12481 tttacgtttg ccctttttct ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt
    12541 tttcgtccca ctccagaaac ggcgcagcag gaaaatcaga ccacccgcaa tgaccaggcc
    12601 gacgataggg gaaacaatca gcgaggagaa aattttggtt acttcacgca ggtttaacgc
    12661 atccatcacc gatgagccgg ttaacagcgc gttggttaaa ccgatgccga taatcgcacc
    12721 aatcaaggtg tgcgaactgg aggccggtaa accaaagaac cacgttccca ggttccagat
    12781 aatcgccgcc agcagcatgg aaaagaccat cgccaaaccg tgagttgacc ccatattcag
    12841 caacaaatcg attggtaaca tatggacaat ggcataggca acgctaagtc cgcccaataa
    12901 cacgccaaaa aagttaaaaa atgccgccat caccacagca agttgtggct gcatggcacg
    12961 agtataaata acggttgcca ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta
    13021 gaacaacaca aatgccagag caagtaataa caaaagcccc gtatatatat caaggccaac
    13081 aaataaattt agcatatatt agattacgcc attttgaatt tacgaacgga cgcattatca
    13141 gtgactttaa cggcatgggc aaagtgaaat atcatttttt aacgataaaa agagtgttat
    13201 ttcttgtgta tcagacaata agcattcatt caaaaccttt agaaataatc aaaacacagg
    13261 tattccactg gttaatatat tcacatatga aatgaataaa tattggaata tataaatatt
    13321 gaatattttg attaatcccc gtactaatta ttcttcataa tcagtacggg gttgctacaa
    13381 catgtattac tttttcacca caattaacgg ttcaatatca ctctcttttt tgatgaccag
    13441 tgattcatca ccgcgcaaac acgtcccacc gtagttgccg ccaacggtga aggtacatac
    13501 ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg
    13561 ctcggcaaat ttaccgctgg ttttgtccag cacctcttca tgatggctga ccaggtcgat
    13621 attgctacca cagcgaccgg cgatcggttt cactgcgtaa cctgttttca ccaattcatc
    13681 attaacagtg aaatcggtat ccagcaggta acgatggtgc gggaacagcg accagaggat
    13741 cggcagaatc gctttgttgc cggggatcac cgtccacagc ggctcaaaga ccagcacttc
    13801 cgggcgcagc aatacgtcga taagacgcac ttcgttttgc ggatgaccgg tacggattgg
    13861 caccgcagca aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc
    13921 ccaggttttc cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc
    13981 atcccagccc agttcatcca gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag
    14041 cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata tctttgtcct gcatgatatg
    14101 gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc
    14161 cgccggattg aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc
    14221 cgcttcggta tgacaggagg cggagtcggc gttgtattcg taaaccttca ggccacgctc
    14281 atccatgcag aaatccatac gaccagtgat catatggtga cggcgacgct gccaggagag
    14341 acgcaaacgt ggccagagga ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt
    14401 cagcaccttg tcggttgcgt gaagatacat cagatgcagc tcgttggtgg ctttaattag
    14461 ctcctgctcg gcactctcgg taatggtgta gtaatgatac ggatcctgat tgatcacctg
    14521 accgttggcc tgcacatagg cgttttgtag cggatctttt tcatccagcc atttaccgtc
    14581 aaactggcct ttgttttcca gacgcgctcc actgattttc agcagctcgc ctgcaatttc
    14641 cggctgcggt aagctgtatt cagtatcttc cgtctggatc atccagccca gaatggtggt
    14701 gtcatcaaaa gtgtctttaa gggtatagca gccgttttcg accaccatct ccagctcgcg
    14761 cgtccactgt tgcccttgcg gcaacgggga atgaatcacg ttctgttccg caatacggac
    14821 tttgttgcca tgcaattggg taatgatggc gacatggcca gtgtctttaa attcaccgcc
    14881 tttatcccag ataagaagcg cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc
    14941 ctgcaatggc aggatgttgt cattaaccac ttctcgcagg aagcgcagcg agaaaatctc
    15001 ccacgccata cccacgtcag taaagaccac accgtaattc agaaagagaa aacggcgagc
    15061 aaattcaacg cattgccact tgtggcccat atattcgtcg tcgatatagc tacggaatac
    15121 ggcgtcatct tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac
    15181 cccacctggg gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt
    15241 gctcatcact ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga
    15301 ttacctgctc agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc
    15361 ggctggcgca aagggttaaa aaattcacat tctgtacagc aagtgacctg ctacactgct
    15421 tcaacactac cactcagaag gcaactcact atgacagaca atacttatca gcccgcgaaa
    15481 gtctggacgt gggataaatc cgctggcggc gcgttcgcca atatcaatcg cccgttttct
    15541 ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacatc cattgcaact ttattcgctg
    15601 ggaacgccga acggtcagaa agtaacgatt atgcttgagg agctactggc gctgggcgtt
    15661 actggtgcag agtacgacgc ctggctgatt cgtattggcg atggcgatca attctccagc
    15721 ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat
    15781 ccgccgatcc gcgtgtttga atctggttcg atcctgcttt atctggcgga gaaatttggc
    15841 tacttcctgc cgcaggatct ggcaaaacgg actgaaacgt tgaactggct gttctggtta
    15901 cagggtgccg cgccgttcct cggcggtggt tttggtcact tttaccatta cgcgccggtg
    15961 aaaattgagt acgccatcaa ccgctttacc atggaagcca agcgcctgct cgacgtgctg
    16021 gataagcaac tggcgcagca taagtttgtt gcgggcgatg agtacaccat tgcggatatg
    16081 gcgatttggc cgtggtttgg caacgtggtg ctcggcggcg tgtatgatgc cgcagagttt
    16141 ctggatgcgg gaagttataa acatgtgcaa cgctgggcga aagaagtggg cgaacgtccg
    16201 gcagtgaaac gtgggcgtat tgttaaccgc accaacgggc cgctgaacga gcagttgcat
    16261 gagcgccatg acgccagtga ttttgagaca aatacagaag ataagcgtca ggggtaaggg
    16321 ttggtgttcg tcgcagcaag ccatccaggc cggataaggc gttcacgccg catccggcaa
    16381 tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa agctaatcat
    16441 cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt ctaatgtggc tttggcttca
    16501 tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca gcacccactg gcccagcaga
    16561 tcggcagggt taccttcgca aatcagggcg atattcacgt cgcgcttgat accacatact
    16621 tcaacctgcg caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca
    16681 atacacattg ttaactccgg ttatttcgat tgccggacaa acgcgaaaaa gaacctttag
    16741 cgggtgttcc ctctcaccct aaccctctcc ccggaggggc gaggggaccg tccgtgcgct
    16801 ttttaggcgt tgtcctcagt cttattcaac ttcaatactt ttgacgatca gcgaatcgcc
    16861 ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat
    16921 ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga cgatatgtaa
    16981 atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac ggacggcgct
    17041 ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc gcttaacatc
    17101 gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg acaactcatg
    17161 cattttcgcc cctcctgcgg ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg
    17221 gattggtgac tggcaacgaa aggatcattc gtgcgcagtc atccgtcagg cgttggcctt
    17281 cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagagg tattgcgaaa
    17341 caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc agaccaattt
    17401 tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata actgcactca
    17461 gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta aaatcagaaa
    17521 catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc cgggcaattt
    17581 cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct atctcttcag
    17641 tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc agccagaacc
    17701 tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat gtgtggctcc
    17761 agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc ggtgaagcgc
    17821 agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt ggtaaacaac
    17881 gccggaactt cttcatcccg caggatcatc atcgttcccg gagtgttctt tttcgacaca
    17941 atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag ctccattccc
    18001 gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg ctctaacgct
    18061 tcgacaatcc gcacgccgat ggcttcatcg gtcagcaaaa tattgccgac ccctaagact
    18121 aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca tccactacgt
    18181 gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact tccagcggtt
    18241 tattcggatc ggcaaccggt gtacccacca gcgactgctc gtaaggaccg acgtcatcat
    18301 tgaagttacg cggaccagag ttccaggttg atggaacaac cgcctggtag ttgctgatga
    18361 taccgtcttt aataaccatc cagtgagaga gcataccgcg cggcgcttcg aggaagccaa
    18421 cacctttgaa ctcacccgtt gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt
    18481 tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc aattcacagc
    18541 agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc acttccagcg
    18601 tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc tgggtagatt
    18661 cgcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct accgttttgc
    18721 cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa ccatcataag
    18781 ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac caggagtgct
    18841 tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg atcggacgat
    18901 acgaggacag atccgcattc tcaatgtagc ctcccgggaa caggaagctg ccgtttttgc
    18961 tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg cgcgtcagcc
    19021 attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc tcaacaaagt
    19081 cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc aacacgccca
    19141 acccgtcgag gttgattggg ttcgcgacac cacccacagc caggttctga atgtgcggcg
    19201 ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc agcgcctgca
    19261 ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc gggtgacccc
    19321 agtagccatt ggcgaaaata cccaactgac cgctggcaac cagatctttg atcttgttct
    19381 gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct ttcagcattt
    19441 cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag tccagcgccg
    19501 aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga atgatgttac
    19561 ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc gcacgaacgg
    19621 aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc atccatgcat
    19681 cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta ccggaagccc
    19741 atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac
    19801 gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat gacgattatg
    19861 atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga tgtaagcgca
    19921 aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg ggaagtaggc
    19981 gtaaccgccg cccgggttga atgccaccaa cgaataggtc agacgccagg ttgcacaacc
    20041 taacagtgcg ctcagtgctg acaggaacag catacgggaa tcattacgca gcttcgccac
    20101 acgcagaacg accagcggga agagcatcag caggacttca atccagaaca tcacggagta
    20161 gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc caaagcgcag
    20221 cccgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga acaggctctt
    20281 ttcatccgga ccgttgccac gcagacccgc ctgcaccagt gaaccttcaa agatgacaat
    20341 cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt catagctctg
    20401 ccagagcgga tgcaccttgt agcccgccga gatcatcagc gaccccattg aagactggtg
    20461 catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt tcagacgttg
    20521 tagcgacacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca gagccatcac
    20581 gccgatgtag atggtcatac agaccgccgt ctcgaacagt accgagttca cgttgaagtg
    20641 acccggaatg tagaagtacg gcaggttcca gtagcgaccc acatcgatag tgatcgacaa
    20701 gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca gcggatggta
    20761 ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac aggcaaagcc
    20821 ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc cgttcaggtc
    20881 agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac agatgacgat
    20941 taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg gttgtggatc
    21001 atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca
    21061 cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg gtgtgttgaa
    21121 tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg agattttcat
    21181 aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg ccgtacagat
    21241 gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg
    21301 gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc gccatcagct
    21361 cttcacgcgt accgaaaatc accgcgcccg ccgggcacac ttctacgcag ccaggcagac
    21421 cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta tgcagcgcac
    21481 caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc atgcagtaac
    21541 ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca
    21601 gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata cactgtttct
    21661 taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg ctggtccaca
    21721 cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc
    21781 cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa gcctggcagc
    21841 ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc gggcggtttt
    21901 cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg caggaggctg
    21961 ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt tatctttctt
    22021 ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca gcccaaccac
    22081 accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag cgttaacatc
    22141 cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga tgcctttatg
    22201 gaagccgata cctttttcgt tacagccata gcacgggtga ccaatcgcca ccggccacac
    22261 accgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg gccctttaca
    22321 gccgaggtgg tacaagcacc agccttcgcg gtggccttca tcaccgaact ctttggcaaa
    22381 acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac ggccataggc
    22441 gaaggtcgga cggtttttgt catccagttt cggcggtttg ccgtaagtga tgatgtgcgc
    22501 aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga taacggtttt
    22561 gcctggcaga acttcttgca agctgactgc gccagttggg ttaactccag ctgcggcaac
    22621 accgccccac gcggagcagg aaccaatagc gataatggct gctgagcctt ccgccgcttt
    22681 gcggatgtga tccacaatcg gctcaccggc aaccatgcaa taaataccgt tatcttttaa
    22741 tgggatggaa ccatccacca ctaacacata ctgccctttg tacttctcga gagcgttatg
    22801 tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat attccagaga
    22861 gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca gagattccgt
    22921 acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg ggttagtaac
    22981 cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg ctaatgctgc
    23041 acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg tgttatctcc
    23101 agtcatttat agttattccg ttgcgagaac ctggcatata ttttgcctca atcgcaaaat
    23161 caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga tatcatgata
    23221 ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta aagcaagata
    23281 atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg caacgtagaa
    23341 caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc atagcggcgc
    23401 gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc gtacatcctg
    23461 tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt cacattaacc
    23521 gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa atggttattc
    23581 atcgttcacc tcattcatta tatatcgcca tatataacga tattttaggc aaagaacgac
    23641 ttttgcgcac gctatttttt catacttgtt taagacgtaa tcccgtaaga gataaaccta
    23701 tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat cggcggcaga
    23761 tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg gatagatata
    23821 agcctcataa accttattat tgtctttcaa cgccgcctcg taaggaggcc agccctcgtt
    23881 gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct tcgccacatc
    23941 ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg gatacgcgac
    24001 agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca ctttacctgt
    24061 cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt
    24121 tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat aacctccaac
    24181 ggaacttaat ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc gccgtgccac
    24241 atcttcgata tacggattca gtccacgatt ctcatgcacc acccccacgg ctggcgtttt
    24301 gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac catttggcga
    24361 aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct gagtcgccag
    24421 cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata
    24481 cttcgccgca aggttgagga actcacgttt cgaaattttc ccgtgaacgt aatagtcgta
    24541 gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg ttgcattcct
    24601 caactggtgt ttttttaagc aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc
    24661 tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa
    24721 attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg ttacgggcag
    24781 atgcagtacc gctattgcgg aaaaagtggt ttacgcctgc ccgcattatc gctcggttta
    24841 tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcaatcct gcgtaaagcg
    24901 tttgatttag gcattacgca ctttgattta gccaacaatt acgggccgcc tccaggaagc
    24961 gcagaagaga actttggtcg cctgctgcgc gaggattttg ctgcttatcg cgatgaactg
    25021 attatctcta ccaaggctgg ctacgatatg tggcccggcc cgtacggctc tggcggttca
    25081 cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct tgagtatgtc
    25141 gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg
    25201 ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca
    25261 gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct tttaattcat
    25321 caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct ggataccctg
    25381 caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt gctgaccgga
    25441 aaatatctca acggcattcc gcaagattca cggatgcatc gtgaagggaa taaagttcgt
    25501 ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt attgaatgaa
    25561 atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt gctgaaagat
    25621 gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga ggagaacgtg
    25681 caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga tcagcatatc
    25741 gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt taataacggg
    25801 cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt caagatagcc
    25861 catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca tcagtttgtt
    25921 atccgggaca tttttcgcat ccataaagac gcgcagtaag tgaatggaag aaattgccac
    25981 aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca gccacttcag
    26041 cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac cggaaaacat
    26101 caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca acacgaggat
    26161 caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct cctggaagaa
    26221 cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg caagcagcca
    26281 gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc gtgtagttaa
    26341 aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc aacatatacg
    26401 ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct tatcaacgca
    26461 tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga tgtctcattt
    26521 aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac aaccgacgcc
    26581 aggcatccag gcgaagatga caccgatacc ggattgcggc gagaaaacct atgttggtag
    26641 cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa taggtcgcgc
    26701 tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga
    26761 agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca aagccgttct
    26821 gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa
    26881 ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg ccattccgga
    26941 tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg ttttcgcgct
    27001 gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagtg tcatcaccac
    27061 ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg ctacgaaggc
    27121 ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag gtattcgggt
    27181 gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg gacaaacgca
    27241 ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga
    27301 actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca ccgcagaagt
    27361 gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac
    27421 cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat ccgcctacaa
    27481 acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg tagcgcatca
    27541 ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta caaacgcacg
    27601 aagattcaac atgttgcaat agattttgta ggcctgatga gcgtagcgca tcaggcaatt
    27661 ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc
    27721 tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg
    27781 aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt aatcattgtt
    27841 tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac cgacagataa
    27901 accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt caccttcaca
    27961 tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa catcacgtcg
    28021 ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga
    28081 tgcattgcca tcggacatta tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca
    28141 gcggcgctgg cttccagatc taggtcacgg ctttgcagca acaatacctg cgctgcaaca
    28201 tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata gataacgacc
    28261 gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc gatacccggc
    28321 gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct gttcatgatg
    28381 ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt tgccagatag
    28441 ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag acggaagctg
    28501 gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag ctcattctgc
    28561 gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc gatatcgttg
    28621 gcctggttta aggaacgtgc ttcagccagc agttgctgct cgcgcttaag gcgacgcttc
    28681 tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac agaggccaaa
    28741 ataagcccaa tcatcacgca cttaacgaca atatcggcgt actgatacat accccagacg
    28801 gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc aagtcacaaa
    28861 atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat tactatttgc
    28921 atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac
    28981 gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg cgaatattca
    29041 tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg acaaaaagct
    29101 tgatactcaa ctggtgaatg caggacgcag caaaaaatac actcttggcg cggtaaatag
    29161 cgtgattcag cgcgcttctt cgctggtatt tgagagtatg gaagccaaaa agcacgcgac
    29221 gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa cccatttttc
    29281 cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg
    29341 ggcggcagcg gttgctaatt cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat
    29401 gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca gcaaactggg
    29461 cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc atctgcagcc
    29521 aaacactaaa atcgtgtttc tggaatcgcc gggttccatc actatggaag tccacgacgt
    29581 tccggcgatt gttgccgccg tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa
    29641 cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg tttctattca
    29701 ggccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg ccgtgtgcaa
    29761 tgcccgttgc tgggagcagc tacgggaaaa tgcctatctg atgggccaga tggtcgatgc
    29821 cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca
    29881 tcatgaaagc agtctgaaag tggccgaatg gctggcagaa catccgcaag ttgcgcgagt
    29941 taaccaccct gccctgcctg gcagtaaagg acacgaattc tggaaacgag actttacagg
    30001 cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa
    30061 ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt atgaatcgtt
    30121 gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggcg agatcgattt
    30181 tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc tgattgccga
    30241 tctggacgcc ggttttgcac ggattgtata acattgccac ttttggacaa ttttgcagac
    30301 attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc ggacgattca
    30361 ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc atggctgtta
    30421 ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg gatcctcata
    30481 ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc
    30541 ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg attgcccaag
    30601 gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt ctgggctgct
    30661 ggctaagtta tattcagggg agctggttag ggaataccaa aacggtgaaa ggctggctgg
    30721 cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc cacggtctgc
    30781 tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca accatggcgg
    30841 gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt ggattgctgt
    30901 gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc gttaaacgcc
    30961 atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta accgctggct
    31021 tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga cgattttccc
    31081 cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg cattcttgcc
    31141 gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg
    31201 ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg gatgatcatc
    31261 atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt gctggtgaca
    31321 gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc cagttgctcg
    31381 acgctcaggt tttcggtgta tttattctca attcgtttca gcacgcggct aatcagactg
    31441 aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg
    31501 tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag tggtcgctcc
    31561 atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt ggcggagtta
    31621 atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc catcaacagt
    31681 tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac ctctgacgtg
    31741 gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc attggcatca
    31801 taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag aattatgatg
    31861 ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca
    31921 tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac tttatccgcc
    31981 agcaagcgac aaatctcttc acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa
    32041 tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag gcaagacatt
    32101 ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac atcaggcaga
    32161 tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa ctttaatctg
    32221 cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt
    32281 cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt
    32341 ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg cggtattgag
    32401 ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga acagaaagtg
    32461 actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt tatcgccgca
    32521 gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg cggtaaagag
    32581 attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc
    32641 aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt ccattctgca
    32701 catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct gccgccgcgt
    32761 caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta tgttaccaaa
    32821 ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa
    32881 gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg
    32941 gcggcgactc aggcgctgaa cggtttgatt ggcactggcg taccgcagga ctgggcaacg
    33001 catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca aacactggct
    33061 atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa
    33121 tatgctgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat tgacgccgcg
    33181 attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct ctccgactac
    33241 ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca cggcatgacc
    33301 caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata cgaagccgcc
    33361 cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga cttaagttca
    33421 caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt atggctaatc
    33481 caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg ggcgtctggc
    33541 aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct
    33601 cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg
    33661 cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac gaccacaagc
    33721 gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat atcgacctct
    33781 acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg aaaggcatga
    33841 tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc
    33901 acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag atcgaacttc
    33961 atccgctgat gcaacaacgc cagctacacg cttggaacgc gacacacaaa atccagaccg
    34021 aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg
    34081 atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg catctggata
    34141 gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa aactttgatg
    34201 tctgggattt ccgtctcgac aaagacgaac tcggcaaaat tgcaaaactc gatcagggca
    34261 agcgtctcgg tcccgatcct gaccagttcg gcggctaacg tgcaaattct cccggtggcg
    34321 gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg
    34381 ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga gccggaagct
    34441 cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga aaaacagatg
    34501 aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc gcagcctcct
    34561 tcacagaccg tagaacaaac agtccggcag atttatcaga actataaatc agatgccagt
    34621 acgccttatt tcggtgaaac cggagagcgg gcgataactt cagcgcgtat tcaacaggcg
    34681 cttaccctga acgacaatct tacactaccg ggcaatattg gctggctgga ttatgatccg
    34741 gtttgtgatt gtcaggattt tggcgatctg gtgttagaaa gcgtagcgat aacccaaact
    34801 gacgtcgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag
    34861 accacacaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga tgatattgtc
    34921 agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct
    34981 ttagcttcgt tgcagaaaga acagccagaa tcctttgttg ctgaactctt tgaacatatt
    35041 gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggttaat
    35101 gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat gcaaatagaa
    35161 cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt
    35221 gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt tcgttttacg
    35281 ctgaccaatg gcaacaatga agagcaagag ctggttttac agcggcgcga aggtaagtgg
    35341 gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa
    35401 accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga acaataaata
    35461 aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg gcaacctgct
    35521 tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat
    35581 taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca caaatctatg
    35641 ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgcacaggac tattttgatt
    35701 gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag catgtcagtt
    35761 gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc accagcattc
    35821 accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg acgctgagtc
    35881 gccatcggcg tatgtttagt caacgccgga cgggtattgc ggttctggcg acgagcttca
    35941 cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc aatcagatgc
    36001 tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg atcgtggtaa
    36061 cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa gacgccttcg
    36121 ctcttataac caatcttcgc cagcggtttt ttcccggtgt aatacatggt ggttgagttc
    36181 gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg ctttttcagc
    36241 cacagcgcca aattcaccat atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac
    36301 gggatcagat actgctcttt acctgcctgt ttcgagtagg tatcgaacag ctctttaaag
    36361 cggtcatagc tgcccatgcc cggcttcatc atcttcgaca acggcccttc ttcggtatgt
    36421 tccggagcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt gatatagcgc
    36481 ggatcttcca cggcgatgtc ataacgtacg cctgaggcga taaggatttt tttaatgcct
    36541 ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt gtccatgtgc
    36601 ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat
    36661 ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat cacgcccgta
    36721 aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg
    36781 ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc gccaaagcag
    36841 ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg ggcattgcca
    36901 taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta
    36961 gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt ttgcatcaat
    37021 gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg ggcgtacagc
    37081 actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt ttcccacggt
    37141 ttctggcgcg gtggctgcac gattacagct ttcgcttcct gctttttcgg cgcaacaggt
    37201 ttgttatccg cacacggcaa atcttcgcca tacggatgcg ggattgggtc gattttccct
    37261 agggtatcaa gacgggtgga atccacaccg ctccagccag gcagcgcctc ttttacgata
    37321 attgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc cagacgatgc
    37381 gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc tttcgaatct
    37441 accagaacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt acggcgcagg
    37501 ctagcctcaa taccgccgag gatcaccggc acatctttcc acgcctcttt acaacgctgg
    37561 gtataaacca gcgtggcgcg atccggtcgc ttacccgcga cattatctgg cgtgtaggca
    37621 tcatcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc catgttgcca
    37681 gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc tttgctgctc
    37741 cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat acgaccgcaa
    37801 atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg
    37861 cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa cggtgccggt
    37921 ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg ttggatcagg
    37981 gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc cggggattat
    38041 aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg ggcgagtatg
    38101 aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc gccatttcca
    38161 gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg aaataaacaa
    38221 gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct tccgggaacg
    38281 gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg aacgcttgcg
    38341 gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg acatcaatac
    38401 ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg atatcgcgac
    38461 tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga agactgtctg
    38521 tgaccagcgg cagaaggccg gttgggcgca gcgttagcac caaggtagaa accagaatac
    38581 tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttca ccacaggtga
    38641 tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg cccggtgcca
    38701 gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca gaaatcacat
    38761 gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac gcgttcagca
    38821 gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca ccgttaacca
    38881 gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt
    38941 tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa tggttgggga
    39001 taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg ccgttataga
    39061 cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct gcgttctggc
    39121 gaataggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc ggaccgccca
    39181 tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct gtcaggcggt
    39241 tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc cccaggtaac
    39301 gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt
    39361 gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga acgggtagcg
    39421 gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa agtgcaatcc
    39481 ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt aaaactggct
    39541 aaagtaatac catgcaggaa agtgttcccc gcaaaaatac agattgccga cttaaacttt
    39601 tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt gttccattat
    39661 cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac tgacgtcaat
    39721 tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat taatcttatt
    39781 cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa
    39841 tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt ccgggaacat
    39901 ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg tgccgtgagc
    39961 tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca actgcccgaa
    40021 gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt gcacaatgtt
    40081 cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag cattgccgta
    40141 gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagtg gcgcaagacg
    40201 gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga aaaggcagta
    40261 aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa gacgaaagat
    40321 atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac ctgaaatcag
    40381 gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa
    40441 taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc tgccggatga
    40501 cgacttaacg tttcatccgg cgttccttgc tagcgggagg aaacagcgcc ctccccggca
    40561 tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc aacacgatcg
    40621 atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt gactttctgt
    40681 agctcttccg gacgcagttt aattttgcgt ttcccaacat gaatggtcag cgtgctttgc
    40741 ggcggcagaa cgtacagttg cgccagacca tcctcgccac gcgcggcttc tgccgatgga
    40801 atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct taccgggaac
    40861 atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc ttcaatcacc
    40921 accggcggca taacatgggc attttccggt aaggtgatca aagccttacc cgcacggtta
    40981 cgcgctacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc
    41041 agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg cggcaacgtt
    41101 aatttgccgg tgagtggttc gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg
    41161 gcatagctac gaccggtgga atcaacaaac actaccggtt ggttactctt acctttcacc
    41221 gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat atcatggcct
    41281 ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc agacggcagc
    41341 atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga acgacgatcg
    41401 tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt cattttacgc
    41461 tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagctcact ctgctcaccg
    41521 cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc
    41581 gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc atcttcgtta
    41641 cgaatgatct caatcacttc gtcgatgttg agaaacgcca ccagcaaacc ttccaggata
    41701 tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac ggtatcgcga
    41761 cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg acgaccatcc
    41821 agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt agcgaagagg
    41881 tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac cagacgggtc
    41941 gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc
    42001 atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg taatgcgctg
    42061 ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga accacgtccg
    42121 ttctcgtaga ttttacgaat ctcggcgcgc gaagtgataa tttccgcttc agtcggataa
    42181 tccggcccct gcacgatatc cagcagctga tcgagcgtag ttttcggctg gtcgattaat
    42241 gcgattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg
    42301 acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgggcagg tagcattttc
    42361 ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg ccccagctcg
    42421 ctcaatagca gctcggaata tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac
    42481 gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg ataacggtaa
    42541 gagaacggct gcgccatcag gaccatcgct tcataacagg cgatatcgcc gtgcggatgg
    42601 tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc gctggcattc
    42661 aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttcag accatcacca
    42721 ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta ggcgttttcc
    42781 gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga ttcctcaact
    42841 tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct gccgcgtcga
    42901 gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga taaaggcatc
    42961 aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg gcagcacaag
    43021 gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc gagaagttga
    43081 ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag tatacggttc
    43141 agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc
    43201 tcatggatat ccccgagacg gcttacggcg caccggataa taaatttcgc gtatacatgg
    43261 tattccttac cggagaaatt ataagcccaa caacaatacg cctgtgacat tgttcgctca
    43321 aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac gccaacggca
    43381 cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg gtgtagtgaa
    43441 acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaataatgc
    43501 cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg gcataacgtc
    43561 caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt tcgctaacgc
    43621 tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcaggggcg tcccaggcga
    43681 cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa
    43741 atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag
    43801 ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt ttgcctccct
    43861 gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg
    43921 atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc ctgaatctca
    43981 accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt tgtgatcgat atcaacattg
    44041 atagtaccgc tggcatcttt aaacacgtag agatcgtcag agatgcgttc aacgatattg
    44101 ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg
    44161 ctgccgttcg gcccctgaaa tcctccggcc tgactttgcg ttaccgatgg gccagaaaaa
    44221 ccgccctgct ctgctgccat caccggtgca ctgcacaggg ccattactgc gattactgct
    44281 gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata
    44341 gtccttaaca acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca
    44401 acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg
    44461 atagaagatg acatgctgat tggtgacggc atcaaaactg gccttagtaa aatgggtttt
    44521 agtgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag cgcaccttat
    44581 gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa
    44641 tggcgagaga aaggtcagcg tgagccggta ctgaacctga ccgcgcgtga tgcgttagcg
    44701 gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg
    44761 atagaagtcg ccgccaggct ggaggctctg atgcgccgaa ccaacggcca ggccagcaac
    44821 gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc
    44881 gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct
    44941 ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc
    45001 accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt
    45061 attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc
    45121 ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc
    45181 tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg ttcgacaccc
    45241 aactgatgct gtttgccaaa cggttaagta cgctcgatct caacgaaatc aacgcggcgg
    45301 atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga
    45361 cctttgccat ctttacccac gacggcagaa tggtccttaa tgatggcgat aacggagaag
    45421 atattcccta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg
    45481 atccttggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggcc
    45541 aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt
    45601 ggctggtcgc actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg
    45661 cgccgctgaa caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac
    45721 taaacgcgac tggcgtaccc agcgaagtgc ggccactggt tgagtcgcta aatcaactgt
    45781 tcgcccgcac acatgcgatg atggttcgag aacgacgctt tacctccgat gcagctcacg
    45841 aactacgtag cccgttaaca gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg
    45901 atgatccgca ggcgcggaaa aaagcactgc tccaattaca ttccgggatc gatcgcgcta
    45961 ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg
    46021 acgtcgcgga gatcccgctt gaagatctcc tacaatcgtc ggtgatggat atttaccaca
    46081 cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc atcaaacgca
    46141 ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac ggccccggtg
    46201 tgacaccaga ggcactggcg cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg
    46261 ctaccggcag tggacttggg ctatcgattg tccagcgaat cgccaagctg catgacatga
    46321 atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc
    46381 tctgtctatg agagccgtta aaacgactct catagatttt actaatagca aaatataaac
    46441 cgtccccaaa aaagccacca accacaaacc agacaaataa cgagcgagaa tacatatagc
    46501 caaaactcga acatttcccg cataaagagt tttcttaaga taagaataat aagtggcgta
    46561 agaagaaaaa atgctgcaat cattgcttgc aatatacgat cttcgcctga aacggaaggt
    46621 atactgcaaa tattgcctcc cgactcaagc atccagtcat acttattgaa agcaaaaaaa
    46681 gaatctatta accaataagt aagcattata tatggcaatg cttcaaatat tgttttaatt
    46741 ttctgcttta acatatatca gtacgctcat aaaaatccat ttataattat caacatgaca
    46801 cgttgatatt ttcatctttt acttattcaa agccatattt tcaatcccca aaaaaaccga
    46861 accaaacgtg taaattattc cggtgcgagt aagaattatg aggaatggct atcagtattg
    46921 tcattttcag aaaatattta tcctgcatcg gtgagtcaga gctagatcag acttttgcta
    46981 aattcgcaaa agactttgca cattttgcta atttcacagt accgctctgt gacgtactat
    47041 agtcgacaaa cgtctcacct tgagattaaa aaatgagcaa catcctgatt atcaacggcg
    47101 cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg
    47161 gcacactgcg cgacctcggg catgatgtcc gcatcgttcg cgccgacagc gactacaatg
    47221 tcaaagcgga agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct
    47281 ggtggatggg cgcgccgtgg acagtgaaaa aatatattga tgatgtattc accgaaggtc
    47341 acgggactct gtatgccagc gatggtcgta cccgcaaaga tccgtcgaaa aaatacggtt
    47401 ccggcagcct ggtacaggac aaaaaatata tgctttctct aacctggaac gcaccaatgg
    47461 aagccttcac cgaaaaagat cagttcttcc acggcgttgg cgttgacggt gtgtatctgc
    47521 cgttccataa agcaaaccaa ttcctcggta tggaaccgct gccgacattt atcgctaatg
    47581 acgtgataaa aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg
    47641 aaatttttgg ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg
    47701 cagaaatccg tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta
    47761 aaatcgttcc aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt
    47821 gcgctgctgg cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt
    47881 gggaaagcat cgcgcacctt gaagcgcatc tgcaaacccc gcacatgaag gcgtacagcg
    47941 aagccgtaaa aggcgacgtg ctggagatga atatccgtat tctgcagcca gggatttaat
    48001 cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc tttcctttaa acctcaatct
    48061 ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc ttccgagcgt ttcttcgcca
    48121 gcagcatatc catcatcgcg tcagtacgct gatcgtcttc atcatcgata gtcaactgca
    48181 ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa ttgcatcggg ttcatttccc
    48241 ccagaccttt aaaacgctgg acgttcggct tgcctttctt gcgttttaat tgctcaagta
    48301 cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc tttcccgaga tcaatacggt
    48361 agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac caacgcgcgg aagtgtttta
    48421 cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc agagtccgca tccgcgagga
    48481 tacagatttt gccataacga agctggctca gatcgtcgct gtcaggatcg ataccgatcg
    48541 ctaccgaaat atcatgcact tcctgcgagg ccagcacttc gtcggaagaa acttcccagg
    48601 tgttaaggat cttacctttc agcggcatga tcgcctgata ttcacgatcg cgcgcctgct
    48661 tggcagatcc gcctgcggag tcaccttcca cgaggaacag ttcggtacgg ttaaggccct
    48721 gcgcggtaca atcagccagt ttgccaggca gcgccgggcc gctggtcagc tttttacgca
    48781 ccactttttt agccgcacgc atacgacgct gggcgctgga aatcgccatc tctgccagca
    48841 actccgccgc ctgaacgttc tggttcagcc acaggatgaa ggcatctttc accacgcccg
    48901 aaacgaatgc cgcacactga cgcgaagaga gacgctcttt ggtctgcccg gcaaactgcg
    48961 gatcctgcat tttaaccgac agaacatagg cgcagcgatc ccagatatct tccgccgaca
    49021 gcttcacgcc gcgcggcaga atgttgcggt attcacagaa ctcacgcatc gcgtccaaca
    49081 ggccctgacg cagaccatta acatgggtac cgccctgcat cgttgggata aggttgacgt
    49141 agctttcggt cagcagttca ccgccttccg gcagccacag tagcgcccag tccacagctt
    49201 cagtatcgcc agcgaaatta ccgataaacg gtttttctgg cagcgtcggt aaaccgttta
    49261 ccgcttccgc caggtaatca ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt
    49321 tgatctcatc tttaaaggta atctcaacgc cggggcacag tactgctttg gccttcagca
    49381 catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca tccggccaga
    49441 agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt aaatcctgca
    49501 ccttttcgcc attttcaaag gcgatgttat aaacctgacc atcgcggcgc acgttaactt
    49561 ctacgcgctt cgacaaggcg ttaactaccg aaatccccac gccatgcagg ccgccagaga
    49621 actggtaatt tttgttagag aatttaccgc ctgcatgcag acggcaaaga atcagttcaa
    49681 ccgccggtac tccttcttcc gggtgaatat ccaccggcat cccgcgccca tcgtcaatca
    49741 cttctaacga ctggtcagca tgtaagatta catccacgcg ttttgcgtga cccgccagtg
    49801 cttcatcgac actgttatca ataacttctt gccccaaatg gttagggcga gtggtatcgg
    49861 tatacatccc cggacggcgg cgaaccggct caagcccggt gagtacctca atggcatcag
    49921 cgttataagt ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca gagatggtgc
    49981 agaccaagaa aatcgacgat cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg
    50041 ccgccttcta tgacagtctg gcggcaggaa gcatagtacg ccaccgcctg gcggtaatcc
    50101 agcacttcat ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca
    50161 atctgcatga ctttgagatc gtaaatatgg cgtgactcta gcacatattg ctgcccggtg
    50221 taggggttct cgttctgacc gagatagtcc gtcagcaatt caaacgggcg taccgccggg
    50281 tttaccacca ctgcgggcag cataaaacat tgcgacaacc aggtggcgta atatcccccc
    50341 agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat ggattccagc
    50401 agttctgccg cgtcggaagg atacggcggc aactgcggaa tgatcatctc aacgtcaggg
    50461 tgatgttccg ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg
    50521 aaaccgtgta aataaagaag cgtagacatc agtagccttc tgaagcggta tcaggttgga
    50581 aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt gccatcagca tgtaactcga
    50641 gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt ggaacagtgc ggcttaaact
    50701 gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga tccagctcct
    50761 gatgaatatg accgcacagc aagtatttga cgtgcggaaa cttcgccagc acggtatcca
    50821 gttcgcccgc gttacgcaga ctgtgttgat cgagccaact acaacccgca ggtagcggat
    50881 gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc ggccagttta cgttccagcc
    50941 actcaagctg aaactcgctc agctcaccgt gcggcacgcc aaacacctgg ctatccagca
    51001 acaggatttg ccattgctca ccaataaaca cccgcttcgc cggggagata cccgcatcct
    51061 gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc cggcagccag acgcagggcg
    51121 cacgaaaact tgcgatgcct tcagcgaaat gctgataggc cgcagaggat tgatcctgcg
    51181 ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg gtgtggacga atcgcctcca
    51241 gcaccgcctg gtagctttcc caggtgttta cccctaacag ggcttcgtgc ttttgtgcaa
    51301 acaggtgagt atcggtaatt tgtaaaatcc ttcctctggc ctcaccagcc agaggaaggg
    51361 ttaacaggct ttccaaatgg tgtccttagg tttcacgacg ctagtaaacc ggaatcgcca
    51421 tcgctccatg tgctaaacag tatcgcaacc agtccgctaa aaactggtta atttgatgct
    51481 tttcgtcgcg ttgatgcaac tttttattag gataatcata ccgcgctttg aagcgaaaaa
    51541 tctgctggct tgaacacact tcagccacca tcgcgtcatg atacagacgc accgtcattg
    51601 acggaaggct ccagtaactg atcgcgggcg cagtctgttc tattgtcacc agggtagtgt
    51661 atcgggtcga ttccacaatc gtcagccgat attgtgcgtt tgccacctga tagcttacag
    51721 tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc
    51781 acaggcgcat catttcagga aagtcaggtg tgtaacgctt catttatgcc cactcatttt
    51841 ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga
    51901 ttttcccctc ttctacccac tggtatgcct gttcccggct taccacatga acgcgaatat
    51961 cttcgttttc atcagccaga ccatgaatac cgcttgcggt cgtggcgtcc acttcgccca
    52021 ccataattga cgaacgctca ctggtgcccc ccgggcttgc caggaaactt aacaccggtt
    52081 tggtccgttt gactatcagt cccgcctctt caatcgcttc gcgacgggca acatcttcca
    52141 cactttcacc ctcttcaatc atcccggcaa ccatctccag tagccagggg gtttcgctgg
    52201 tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac ttcatcacgc actgggtcaa
    52261 agggtagcaa gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc tcatgactca
    52321 tttgcccgtt gaatagacga tgacgaaatc tataaagatc taatgaaaaa aagccgcgat
    52381 aaagtgtttc tcgtgcaata atttctacat cgtttttgcc aaatgtaacg ggcaggttgt
    52441 ctggcttaag cattgttaat gtcctagcac taatagtgaa ttaaatgtga atttcagcga
    52501 cgtttgactg ccgtttgagc agtcatatgt taaattgagg cacattaacg ccctatggca
    52561 cgtaacgcca accttttgcg gtagcggctt ctgctagaat ccgcaataat tttacagttt
    52621 gatcgcgcta aatactgctt caccacaagg aatgcaaatg aagaaattgc tccccattct
    52681 tatcggcctg agcctttctg ggttcagttc gttgagccag gccgagaacc tgatgcaagt
    52741 ttatcagcaa gcacgcctta gtaacccgga attgcgtaag tctgccgccg atcgtgatgc
    52801 tgcctttgaa aaaattaatg aagcgcgcag tccattactg ccacagctag gtttaggtgc
    52861 agattacacc tatagcaacg gctaccgcga cgcgaacggc atcaactcga acgcgaccag
    52921 tgcgtccctg cagttaactc aatccatttt tgatatgtcg aaatggcgtg cgttaacgct
    52981 gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag accgatcagc aaaccttgat
    53041 cctcaacacc gcgaccgctt atttcaacgt gttgaatgct attgacgttc tttcctatac
    53101 acaggcacaa aaagaagcga tctaccgtca attagatcaa accacccaac gttttaacgt
    53161 gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg cagtacgata ccgtgctggc
    53221 gaacgaagtg accgcacgta ataaccttga taacgcggta gagcagctgc gccagatcac
    53281 cggtaactac tatccggaac tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc
    53341 acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc aacctgtcgc tgttacaggc
    53401 acgcttgagc caggacctgg agcgcgagca aattcgccag gcgcaggatg gtcacttacc
    53461 gactctggat ttaacggctt ctaccgggat ttctgacacc tcttatagcg gttcgaaaac
    53521 ccgtggtgcc gctggtaccc agtatgacga tagcaatatg ggccagaaca aagttggcct
    53581 gagcttctcg ctgccgattt atcagggcgg aatggttaac tcgcaggtga aacaggcaca
    53641 gtacaacttt gttggtgcca gtgagcaact ggaaagcgca catcgtagcg tcgtgcaaac
    53701 cgtacgttcc tccttcaaca acattaatgc ttctatcagt agtattaacg cctacaaaca
    53761 agccgtagtt tccgcgcaaa gctcattaga cgcgatggaa gcgggctact cggtcggtac
    53821 gcgtaccatt gttgatgtgt tggatgcgac caccacgctg tacaacgcca agcaagagct
    53881 ggcgaatgcg cgttataact acctgattaa tcagctgaat attaagtcag ccctgggtac
    53941 gttgaacgag caggatttgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa
    54001 tccggaaaac gttgccccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc
    54061 tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca
    54121 taaccctttc cgtaactgat gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa
    54181 cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc
    54241 atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa
    54301 atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga caccagtcgc
    54361 tctcgcggtt gccactgttc ttatgctggc tggctgtgaa aagagtgatg aaacagtgtc
    54421 tctctatcaa aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac
    54481 caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat acgccacccg
    54541 tgaagactgt gttgctgaat ttggtgaagg tcagtgtcag caagcaccag cccaggctgg
    54601 catggcacca gaaaaccagg cgcaggctca gcaatccagc gggagtttct ggatgccgct
    54661 gatggccggt tacatgatgg ggcgtctgat gggcggcggc gcgggatttg cacagcagcc
    54721 gctgttctcc tcgaaaaacc cggccagtcc ggcttacggt aaatataccg acgcgacggg
    54781 taaaaactat ggcgcagccc agccaggccg caccatgacc gtaccgaaga cggcaatggc
    54841 accaaaaccg gcgaccacca ctaccgttac ccgtggcggt tttggtgaat ctgttgccaa
    54901 acaaagcact atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg gtggctgata
    54961 ccgatggaaa gagtcagtat taccgagcgc ccggactggc gtgagaaagc ccacgaatac
    55021 ggtttcaatt ttcacaccat gtacggcgag ccgtactggt gtgaagatgc ttactacaag
    55081 ttgaccctcg cccaggttga aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc
    55141 ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga tgaccaaatt ccgcatacca
    55201 aaacacacct ggagttttgt gcgtcaatca tggctgactc accagccatc gctttattcg
    55261 cgtcttgatc tggcgtggga tggcactggc gaacctaaac ttctggaaaa taacgccgat
    55321 acgccaacgt cactatacga ggcggcgttc tttcagtgga tctggctgga agatcagctt
    55381 aacgccggta acttgccgga gggcagcgac cagtttaaca gtctgcaaga aaaactgatc
    55441 gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt tgctgcatct cacctgctgt
    55501 cgcgacacgg tggaagatcg cggaaccatt cagtatttgc aggactgcgc aaccgaagct
    55561 gaaatcgcca ccgagttcct ctacatcgac gagataggtt taggcgaaaa aggccagttc
    55621 acggatttac aggatcaggt gatttctaac ctgttcaagc tttatccgtg ggaatttatg
    55681 ctgcgcgaaa tgttctccac caagctggaa gatgctggcg tacgctggct ggaaccggcg
    55741 tggaagagca ttatctccaa caaggcactt ctaccgctac tgtgggagat gttcccgaat
    55801 cacccgaacc tgctgcccgc ttattttgcg gaagatgatc atccgcaaat ggaaaaatat
    55861 gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa
    55921 accattgaag cagcggaagg tccgtatggc gaagaaggga tgattgttca gcaattccac
    55981 ccgttaccga aattcggcga cagctatatg ctgattggta gctggctggt gaacgatcaa
    56041 cctgccggaa ttggcattcg tgaagaccgt gcgttgatca cccaggatat gtctcggttt
    56101 tatccacata tttttgttga ataagccacg ataccggatg gcactcgcca tccggtaatt
    56161 gttagcctat ctgcaccgac agcatactta aactgcccat ttctatacca tcgaccggaa
    56221 tggtaattgg ctcctgccca tcccacacac ctaacacata caacaacggc agatagtgct
    56281 ctggcgttgg gttcgataac gcgccacctt catggtcgag ataattcacc agaggatgct
    56341 gctctaccgg cccttgccac gtcagattcg ctttcacata ctcattaaac gacgtcgccc
    56401 acggatacgg tgaactatca ccgtgccact tcactgtgcg caggttatgc accacgttac
    56461 cgctggcgac caacattatt ccttcatcac gcagcgccgc gagtttgcgc cccatttcga
    56521 aatgccaggc ggcaggtttg ctactgtcga tactcaactg caccataggg atatcggcgt
    56581 caggatacat cttaatcagc acgccccacg agccgtggtc aaagccccag gcttctttat
    56641 ccagcgtcac cgggatcggc gctaacagct caaccagacg ctgtgccagc gcaggcgaac
    56701 ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg
    56761 tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt aaaccagtga gccgaaacca
    56821 ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg cgggtataca
    56881 aattatcttc cagcacgttc atcggactac cgtgacctaa aaacaatgct ggcatacgtg
    56941 ttgaagacat gatgatatcc ttaactaaag gtgtcatttt gatatcctca caatacgctt
    57001 gttcggcgga gtaagaaccc ggataacaat gatgatgatc atcagttatt ttgacgatct
    57061 gcctgaaggt gaagatttat aaggagttgt cgatgtcagt acctctcatt ctgaccatac
    57121 tggcgggggc agccacgttt attggcgcgt ttctcggcgt tctcggacaa aaaccctcga
    57181 accgcttact ggcgttttcg ctaggttttg cggcggggat catgttgctc atctcattaa
    57241 tggaaatgct tcctgccgca ctagcagctg aaggaatgtc gccggtgttg ggttatggaa
    57301 tgtttatctt cggtctgctt ggctactttg gcctggaccg catgttgccg catgcgcatc
    57361 cgcaggattt aatgcaaaaa tcggtgcagc tgttgccaaa atcgatcaag cgcacagcca
    57421 ttctgctcac tctcggcatc agtctgcata acttcccgga agggattgcc acctttgtca
    57481 cggcgagcag caacctggag ctgggatttg gcatcgcact ggccgtcgcg ttgcacaata
    57541 tccctgaagg tctggcagtg gcaggcccgg tttatgcggc aacggggtct aaacgcaccg
    57601 cgattctgtg ggcggggatt tctggactag cagaaattct tggtggtgtg ctggcgtggt
    57661 taatcctcgg tagcatgatt tccccggtgg tcatggcggc aatcatggcg gcggttgcag
    57721 gaattatggt ggcgctctcg gttgatgaat taatgccgct cgccaaagag attgacccta
    57781 ataataaccc cagctatggc gtactatgtg gaatgtcagt gatgggattc agtttagtgc
    57841 tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc acatggacaa
    57901 cttctgccag cttcacgaaa gccttattct accttttcgg ctgtctcttc tctcgtactg
    57961 tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa
    58021 taacgtttca aatgcaggaa ataacccacc acaaccagag caatcaatac aatccagtac
    58081 caggcgataa acatcctttt acccttaata tttaacaata ctaaacattt agcgcataaa
    58141 tttcacatat cctttttcgg atatattcat atggtcgcat aataacggaa caattatatg
    58201 attttgatca taatgaaaaa gaatgcctga tattaatgaa attttctcat agaaagagaa
    58261 gcggaattaa catcttgcat agcagggcaa aataaaaacc gaagccagct acaacttcgg
    58321 tttcttgatt aaggcagtaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct
    58381 gacggtatgc caccaggtct tcaatagtca cgagcgccat attgtgttta ttggcaaact
    58441 caatgcactc tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac
    58501 cagccggttt aaagcctgcc agcgtcatca aatcaatagt cgcttcagta tgaccgccac
    58561 gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat
    58621 cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag
    58681 aaacaccggt agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg
    58741 cgctggtgtt attttctacc atcattggca gatcaagttg tttacggcga tcttcagtga
    58801 tgcacaggca aacaataccg ctaccgtggc gaatggtcag cgccatctgc tcaacggtca
    58861 tggtttctgc cgggaagatc atatcgcctt cgttttcacg gtcttcatca tcaagcacca
    58921 ttacaccgcg tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac
    58981 caaaagagga aagtagcgtc tgattcatgg taaaaaaaac ctcactaaaa ttatggttac
    59041 cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat
    59101 gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac
    59161 cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat
    59221 ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact ataacgctaa
    59281 tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc
    59341 actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa
    59401 attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg
    59461 gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt
    59521 gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg
    59581 gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag
    59641 ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt
    59701 atgaattaat cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga
    59761 gtatcatcac aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt
    59821 tttacttaat cgata
//
LOCUS       DAZSNG010000013        58573 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZSNG010000013 DAZSNG010000000
VERSION     DAZSNG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58573
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.013"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1101)
                     /locus_tag="ACHW4Z_000827"
     CDS             complement(<1..1101)
                     /locus_tag="ACHW4Z_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8880586.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHW4Z_000828"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHW4Z_000828"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR8880587.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHW4Z_000829"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHW4Z_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR8880588.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHW4Z_000830"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHW4Z_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR8880589.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHW4Z_000831"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHW4Z_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR8880590.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHW4Z_000832"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHW4Z_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR8880591.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHW4Z_000833"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHW4Z_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR8880592.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHW4Z_000834"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHW4Z_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR8880593.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHW4Z_000835"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHW4Z_000835"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR8880594.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHW4Z_000836"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHW4Z_000836"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR8880595.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHW4Z_000837"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHW4Z_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR8880596.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHW4Z_000838"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHW4Z_000838"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR8880597.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHW4Z_000839"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHW4Z_000839"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR8880598.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHW4Z_000840"
     CDS             11851..12015
                     /locus_tag="ACHW4Z_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880599.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHW4Z_000841"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHW4Z_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR8880600.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHW4Z_000842"
     CDS             13524..13769
                     /locus_tag="ACHW4Z_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR8880601.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHW4Z_000843"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHW4Z_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR8880602.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHW4Z_000844"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHW4Z_000844"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR8880603.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHW4Z_000845"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHW4Z_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR8880604.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHW4Z_000846"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHW4Z_000846"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR8880605.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHW4Z_000847"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHW4Z_000847"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR8880606.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHW4Z_000848"
     CDS             19432..19785
                     /locus_tag="ACHW4Z_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR8880607.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHW4Z_000849"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHW4Z_000849"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR8880608.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHW4Z_000850"
     CDS             21085..22212
                     /locus_tag="ACHW4Z_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR8880609.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHW4Z_000851"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHW4Z_000851"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR8880610.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHW4Z_000852"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHW4Z_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHW4Z_000853"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHW4Z_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHW4Z_000854"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHW4Z_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR8880611.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHW4Z_000855"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHW4Z_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR8880612.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHW4Z_000856"
     CDS             26834..27583
                     /locus_tag="ACHW4Z_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR8880613.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHW4Z_000857"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHW4Z_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR8880614.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHW4Z_000858"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHW4Z_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR8880615.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHW4Z_000859"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHW4Z_000859"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR8880616.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHW4Z_000860"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHW4Z_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR8880617.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHW4Z_000861"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHW4Z_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR8880618.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHW4Z_000862"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHW4Z_000862"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR8880619.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHW4Z_000863"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHW4Z_000863"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR8880620.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHW4Z_000864"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHW4Z_000864"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR8880621.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHW4Z_000865"
     CDS             complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHW4Z_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR8880622.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHW4Z_000866"
     CDS             complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHW4Z_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR8880623.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36031)
                     /locus_tag="ACHW4Z_000867"
     CDS             complement(35891..36031)
                     /locus_tag="ACHW4Z_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR8880624.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHW4Z_000868"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHW4Z_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR8880625.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37527..40130)
                     /gene="topA"
                     /locus_tag="ACHW4Z_000869"
     CDS             complement(37527..40130)
                     /gene="topA"
                     /locus_tag="ACHW4Z_000869"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR8880626.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKNNL"
     gene            40510..40761
                     /locus_tag="ACHW4Z_000870"
     CDS             40510..40761
                     /locus_tag="ACHW4Z_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR8880627.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="ACHW4Z_000871"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="ACHW4Z_000871"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR8880628.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="ACHW4Z_000872"
     CDS             42066..42824
                     /locus_tag="ACHW4Z_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR8880629.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="ACHW4Z_000873"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="ACHW4Z_000873"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR8880630.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="ACHW4Z_000874"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="ACHW4Z_000874"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR8880631.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="ACHW4Z_000875"
     CDS             complement(44424..45044)
                     /locus_tag="ACHW4Z_000875"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR8880632.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="rnm"
                     /locus_tag="ACHW4Z_000876"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(45041..45922)
                     /gene="rnm"
                     /locus_tag="ACHW4Z_000876"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR8880633.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="ACHW4Z_000877"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="ACHW4Z_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR8880634.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="ACHW4Z_000878"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="ACHW4Z_000878"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR8880635.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="ACHW4Z_000879"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="ACHW4Z_000879"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR8880636.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="ACHW4Z_000880"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="ACHW4Z_000880"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR8880637.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="ACHW4Z_000881"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="ACHW4Z_000881"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR8880638.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="ACHW4Z_000882"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="ACHW4Z_000882"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR8880639.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="ACHW4Z_000883"
     CDS             53106..53285
                     /locus_tag="ACHW4Z_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR8880640.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="ACHW4Z_000884"
     CDS             53371..53871
                     /locus_tag="ACHW4Z_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8880641.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="ACHW4Z_000885"
     CDS             53917..54423
                     /locus_tag="ACHW4Z_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8880642.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="ACHW4Z_000886"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="ACHW4Z_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR8880643.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="ACHW4Z_000887"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="ACHW4Z_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="ACHW4Z_000888"
     CDS             55478..56221
                     /locus_tag="ACHW4Z_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR8880644.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="ACHW4Z_000889"
     CDS             56251..56790
                     /locus_tag="ACHW4Z_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR8880645.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="ACHW4Z_000890"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="ACHW4Z_000890"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR8880646.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="ACHW4Z_000891"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="ACHW4Z_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR8880647.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="ACHW4Z_000892"
     CDS             58276..58572
                     /locus_tag="ACHW4Z_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR8880648.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14061 a        15144 c        14644 g        14724 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaactcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggatttttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gag
//
LOCUS       DAZSNG010000014        58031 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZSNG010000014 DAZSNG010000000
VERSION     DAZSNG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58031
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.014"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(24..278)
                     /locus_tag="ACHW4Z_000893"
     CDS             complement(24..278)
                     /locus_tag="ACHW4Z_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR8880649.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHW4Z_000894"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHW4Z_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR8880650.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHW4Z_000895"
     CDS             1228..2694
                     /locus_tag="ACHW4Z_000895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8880651.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHW4Z_000896"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHW4Z_000896"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR8880652.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHW4Z_000897"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHW4Z_000897"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR8880653.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHW4Z_000898"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHW4Z_000898"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR8880654.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHW4Z_000899"
     CDS             5709..7265
                     /locus_tag="ACHW4Z_000899"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR8880655.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHW4Z_000900"
     CDS             7347..9161
                     /locus_tag="ACHW4Z_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR8880656.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHW4Z_000901"
     CDS             9162..10256
                     /locus_tag="ACHW4Z_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR8880657.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHW4Z_000902"
     CDS             10256..11281
                     /locus_tag="ACHW4Z_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8880658.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHW4Z_000903"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHW4Z_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR8880659.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHW4Z_000904"
     CDS             complement(12876..13220)
                     /locus_tag="ACHW4Z_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR8880660.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACHW4Z_000905"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACHW4Z_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR8880661.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACHW4Z_000906"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACHW4Z_000906"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR8880662.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="ACHW4Z_000907"
     CDS             15616..17376
                     /locus_tag="ACHW4Z_000907"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR8880663.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="ACHW4Z_000908"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="ACHW4Z_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR8880664.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACHW4Z_000909"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACHW4Z_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR8880665.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="ACHW4Z_000910"
     CDS             19113..19340
                     /locus_tag="ACHW4Z_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR8880666.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="yejM"
                     /locus_tag="ACHW4Z_000911"
     CDS             19360..21120
                     /gene="yejM"
                     /locus_tag="ACHW4Z_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR8880667.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="ACHW4Z_000912"
     tRNA            21195..21271
                     /locus_tag="ACHW4Z_000912"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACHW4Z_000913"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACHW4Z_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR8880668.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="ACHW4Z_000914"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="ACHW4Z_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR8880669.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACHW4Z_000915"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACHW4Z_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR8880670.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACHW4Z_000916"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACHW4Z_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR8880671.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACHW4Z_000917"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACHW4Z_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR8880672.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACHW4Z_000918"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACHW4Z_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR8880673.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACHW4Z_000919"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACHW4Z_000919"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR8880674.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACHW4Z_000920"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACHW4Z_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR8880675.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACHW4Z_000921"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACHW4Z_000921"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR8880676.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACHW4Z_000922"
     CDS             complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACHW4Z_000922"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR8880677.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACHW4Z_000923"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACHW4Z_000923"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR8880678.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACHW4Z_000924"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACHW4Z_000924"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR8880679.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACHW4Z_000925"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACHW4Z_000925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR8880680.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACHW4Z_000926"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACHW4Z_000926"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR8880681.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACHW4Z_000927"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACHW4Z_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR8880682.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACHW4Z_000928"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACHW4Z_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR8880683.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACHW4Z_000929"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACHW4Z_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR8880684.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /gene="yojO"
                     /locus_tag="ACHW4Z_000930"
     CDS             37391..37555
                     /gene="yojO"
                     /locus_tag="ACHW4Z_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR8880685.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37669..38178
                     /gene="eco"
                     /locus_tag="ACHW4Z_000931"
     CDS             37669..38178
                     /gene="eco"
                     /locus_tag="ACHW4Z_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR8880686.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACHW4Z_000932"
                     /pseudo
     CDS             complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACHW4Z_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="ACHW4Z_000933"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="ACHW4Z_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR8880687.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="ACHW4Z_000934"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="ACHW4Z_000934"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR8880688.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="ACHW4Z_000935"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="ACHW4Z_000935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR8880689.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="ACHW4Z_000936"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="ACHW4Z_000936"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR8880690.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="ACHW4Z_000937"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="ACHW4Z_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8880691.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="ACHW4Z_000938"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="ACHW4Z_000938"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR8880692.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="ACHW4Z_000939"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="ACHW4Z_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR8880693.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="ACHW4Z_000940"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="ACHW4Z_000940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR8880694.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="ACHW4Z_000941"
     CDS             complement(53338..54114)
                     /locus_tag="ACHW4Z_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR8880695.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="ACHW4Z_000942"
     CDS             complement(54119..55768)
                     /locus_tag="ACHW4Z_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR8880696.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..58031)
                     /locus_tag="ACHW4Z_000943"
                     /pseudo
     CDS             complement(55769..58031)
                     /locus_tag="ACHW4Z_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14198 a        15655 c        14257 g        13921 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtagc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga cgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta a
//
LOCUS       DAZSNG010000015        55420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZSNG010000015 DAZSNG010000000
VERSION     DAZSNG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.015"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHW4Z_000944"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHW4Z_000944"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR8880697.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHW4Z_000945"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHW4Z_000945"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR8880698.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHW4Z_000946"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHW4Z_000946"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR8880699.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHW4Z_000947"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHW4Z_000947"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR8880700.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHW4Z_000948"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHW4Z_000948"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR8880701.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHW4Z_000949"
     CDS             7826..9559
                     /locus_tag="ACHW4Z_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR8880702.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHW4Z_000950"
     CDS             9708..10559
                     /locus_tag="ACHW4Z_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8880703.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHW4Z_000951"
     CDS             complement(10546..10887)
                     /locus_tag="ACHW4Z_000951"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8880704.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHW4Z_000952"
     CDS             complement(10889..11767)
                     /locus_tag="ACHW4Z_000952"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR8880705.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHW4Z_000953"
     CDS             complement(11733..14030)
                     /locus_tag="ACHW4Z_000953"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8880706.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHW4Z_000954"
     CDS             complement(14081..14401)
                     /locus_tag="ACHW4Z_000954"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8880707.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHW4Z_000955"
     CDS             complement(14416..15495)
                     /locus_tag="ACHW4Z_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR8880708.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHW4Z_000956"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHW4Z_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHW4Z_000957"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHW4Z_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHW4Z_000958"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHW4Z_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHW4Z_000959"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHW4Z_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHW4Z_000960"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHW4Z_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR8880709.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHW4Z_000961"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHW4Z_000961"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR8880710.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHW4Z_000962"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHW4Z_000962"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR8880711.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHW4Z_000963"
     CDS             25474..25608
                     /locus_tag="ACHW4Z_000963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880712.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHW4Z_000964"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHW4Z_000964"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR8880713.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHW4Z_000965"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHW4Z_000965"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR8880714.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHW4Z_000966"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHW4Z_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR8880715.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHW4Z_000967"
     CDS             30120..30728
                     /locus_tag="ACHW4Z_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR8880716.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHW4Z_000968"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHW4Z_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR8880717.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHW4Z_000969"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHW4Z_000969"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR8880718.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHW4Z_000970"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHW4Z_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR8880719.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHW4Z_000971"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHW4Z_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR8880720.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHW4Z_000972"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHW4Z_000972"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR8880721.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHW4Z_000973"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHW4Z_000973"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR8880722.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHW4Z_000974"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHW4Z_000974"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR8880723.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHW4Z_000975"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHW4Z_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR8880724.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHW4Z_000976"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHW4Z_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR8880725.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHW4Z_000977"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHW4Z_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHW4Z_000978"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHW4Z_000978"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR8880726.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHW4Z_000979"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHW4Z_000979"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8880727.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHW4Z_000980"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHW4Z_000980"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR8880728.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHW4Z_000981"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHW4Z_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR8880729.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHW4Z_000982"
     CDS             complement(44843..45142)
                     /locus_tag="ACHW4Z_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR8880730.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHW4Z_000983"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHW4Z_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHW4Z_000984"
     CDS             45895..46494
                     /locus_tag="ACHW4Z_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR8880731.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHW4Z_000985"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHW4Z_000985"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR8880732.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHW4Z_000986"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHW4Z_000986"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR8880733.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHW4Z_000987"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHW4Z_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR8880734.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHW4Z_000988"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHW4Z_000988"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR8880735.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHW4Z_000989"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHW4Z_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR8880736.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHW4Z_000990"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHW4Z_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR8880737.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHW4Z_000991"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHW4Z_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR8880738.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHW4Z_000992"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHW4Z_000992"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR8880739.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHW4Z_000993"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHW4Z_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR8880740.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14166 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcggggtca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAZSNG010000016        55370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZSNG010000016 DAZSNG010000000
VERSION     DAZSNG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55370
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.016"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..358
                     /locus_tag="ACHW4Z_000994"
     CDS             <1..358
                     /locus_tag="ACHW4Z_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8880741.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="ACHW4Z_000995"
     CDS             complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="ACHW4Z_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR8880742.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="ACHW4Z_000996"
     CDS             complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="ACHW4Z_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR8880743.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3113..3265
                     /gene="mokC"
                     /locus_tag="ACHW4Z_000997"
     CDS             3113..3265
                     /gene="mokC"
                     /locus_tag="ACHW4Z_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR8880744.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="ACHW4Z_000998"
     CDS             complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="ACHW4Z_000998"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR8880745.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="ACHW4Z_000999"
     CDS             complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="ACHW4Z_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR8880746.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6881..7285
                     /locus_tag="ACHW4Z_001000"
     CDS             6881..7285
                     /locus_tag="ACHW4Z_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR8880747.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7311..8024
                     /gene="msyB"
                     /locus_tag="ACHW4Z_001001"
     CDS             7311..8024
                     /gene="msyB"
                     /locus_tag="ACHW4Z_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR8880748.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8173..8739
                     /gene="satP"
                     /locus_tag="ACHW4Z_001002"
     CDS             8173..8739
                     /gene="satP"
                     /locus_tag="ACHW4Z_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR8880749.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8774..9361)
                     /gene="mog"
                     /locus_tag="ACHW4Z_001003"
     CDS             complement(8774..9361)
                     /gene="mog"
                     /locus_tag="ACHW4Z_001003"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR8880750.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9476..10429)
                     /gene="tal"
                     /locus_tag="ACHW4Z_001004"
     CDS             complement(9476..10429)
                     /gene="tal"
                     /locus_tag="ACHW4Z_001004"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8880751.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10708..12138
                     /locus_tag="ACHW4Z_001005"
                     /pseudo
     CDS             10708..12138
                     /locus_tag="ACHW4Z_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12208..12984
                     /gene="yaaA"
                     /locus_tag="ACHW4Z_001006"
     CDS             12208..12984
                     /gene="yaaA"
                     /locus_tag="ACHW4Z_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR8880752.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13137..13433)
                     /locus_tag="ACHW4Z_001007"
     CDS             complement(13137..13433)
                     /locus_tag="ACHW4Z_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR8880753.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="ACHW4Z_001008"
     CDS             complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="ACHW4Z_001008"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR8880754.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="ACHW4Z_001009"
     CDS             complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="ACHW4Z_001009"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR8880755.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="ACHW4Z_001010"
     CDS             complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="ACHW4Z_001010"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR8880756.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18356..18475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18411..18476)
                     /gene="thrL"
                     /locus_tag="ACHW4Z_001011"
     CDS             complement(18411..18476)
                     /gene="thrL"
                     /locus_tag="ACHW4Z_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR8880757.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18690..19376)
                     /locus_tag="ACHW4Z_001012"
     CDS             complement(18690..19376)
                     /locus_tag="ACHW4Z_001012"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8880758.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19776..19916)
                     /gene="yjjY"
                     /locus_tag="ACHW4Z_001013"
     CDS             complement(19776..19916)
                     /gene="yjjY"
                     /locus_tag="ACHW4Z_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR8880759.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20012..20728
                     /gene="arcA"
                     /locus_tag="ACHW4Z_001014"
     CDS             20012..20728
                     /gene="arcA"
                     /locus_tag="ACHW4Z_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR8880760.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20787..22139)
                     /gene="creD"
                     /locus_tag="ACHW4Z_001015"
     CDS             complement(20787..22139)
                     /gene="creD"
                     /locus_tag="ACHW4Z_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR8880761.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22197..23621)
                     /gene="creC"
                     /locus_tag="ACHW4Z_001016"
     CDS             complement(22197..23621)
                     /gene="creC"
                     /locus_tag="ACHW4Z_001016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR8880762.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23621..24310)
                     /gene="creB"
                     /locus_tag="ACHW4Z_001017"
     CDS             complement(23621..24310)
                     /gene="creB"
                     /locus_tag="ACHW4Z_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR8880763.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24323..24796)
                     /gene="creA"
                     /locus_tag="ACHW4Z_001018"
     CDS             complement(24323..24796)
                     /gene="creA"
                     /locus_tag="ACHW4Z_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR8880764.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25007..25876
                     /gene="robA"
                     /locus_tag="ACHW4Z_001019"
     CDS             25007..25876
                     /gene="robA"
                     /locus_tag="ACHW4Z_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR8880765.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25873..26520)
                     /gene="gpmB"
                     /locus_tag="ACHW4Z_001020"
     CDS             complement(25873..26520)
                     /gene="gpmB"
                     /locus_tag="ACHW4Z_001020"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR8880766.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26572..27092
                     /gene="yjjX"
                     /locus_tag="ACHW4Z_001021"
                     /pseudo
     CDS             26572..27092
                     /gene="yjjX"
                     /locus_tag="ACHW4Z_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27177..27503)
                     /gene="trpR"
                     /locus_tag="ACHW4Z_001022"
     CDS             complement(27177..27503)
                     /gene="trpR"
                     /locus_tag="ACHW4Z_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR8880767.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27593..29530)
                     /gene="sltY"
                     /locus_tag="ACHW4Z_001023"
     CDS             complement(27593..29530)
                     /gene="sltY"
                     /locus_tag="ACHW4Z_001023"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR8880768.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29741..31408
                     /gene="ettA"
                     /locus_tag="ACHW4Z_001024"
     CDS             29741..31408
                     /gene="ettA"
                     /locus_tag="ACHW4Z_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR8880769.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31515..31682)
                     /gene="nadS"
                     /locus_tag="ACHW4Z_001025"
     CDS             complement(31515..31682)
                     /gene="nadS"
                     /locus_tag="ACHW4Z_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR8880770.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31716..32948)
                     /gene="nadR"
                     /locus_tag="ACHW4Z_001026"
     CDS             complement(31716..32948)
                     /gene="nadR"
                     /locus_tag="ACHW4Z_001026"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR8880771.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32969..34351)
                     /gene="radA"
                     /locus_tag="ACHW4Z_001027"
     CDS             complement(32969..34351)
                     /gene="radA"
                     /locus_tag="ACHW4Z_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR8880772.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34400..35368)
                     /gene="serB"
                     /locus_tag="ACHW4Z_001028"
     CDS             complement(34400..35368)
                     /gene="serB"
                     /locus_tag="ACHW4Z_001028"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR8880773.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35474..36118
                     /locus_tag="ACHW4Z_001029"
     CDS             35474..36118
                     /locus_tag="ACHW4Z_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR8880774.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36146..37161
                     /gene="lplA"
                     /locus_tag="ACHW4Z_001030"
                     /pseudo
     CDS             36146..37161
                     /gene="lplA"
                     /locus_tag="ACHW4Z_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37193..37456
                     /locus_tag="ACHW4Z_001031"
     CDS             37193..37456
                     /locus_tag="ACHW4Z_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8880775.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37617..38336)
                     /gene="deoD"
                     /locus_tag="ACHW4Z_001032"
     CDS             complement(37617..38336)
                     /gene="deoD"
                     /locus_tag="ACHW4Z_001032"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR8880776.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38393..39616)
                     /gene="deoB"
                     /locus_tag="ACHW4Z_001033"
     CDS             complement(38393..39616)
                     /gene="deoB"
                     /locus_tag="ACHW4Z_001033"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8880777.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39668..40990)
                     /gene="deoA"
                     /locus_tag="ACHW4Z_001034"
     CDS             complement(39668..40990)
                     /gene="deoA"
                     /locus_tag="ACHW4Z_001034"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR8880778.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41117..41896)
                     /gene="deoC"
                     /locus_tag="ACHW4Z_001035"
     CDS             complement(41117..41896)
                     /gene="deoC"
                     /locus_tag="ACHW4Z_001035"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR8880779.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42154..43695
                     /locus_tag="ACHW4Z_001036"
     CDS             42154..43695
                     /locus_tag="ACHW4Z_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR8880780.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43667..44530
                     /locus_tag="ACHW4Z_001037"
     CDS             43667..44530
                     /locus_tag="ACHW4Z_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR8880781.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44744..45526)
                     /locus_tag="ACHW4Z_001038"
     CDS             complement(44744..45526)
                     /locus_tag="ACHW4Z_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8880782.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45523..46596)
                     /locus_tag="ACHW4Z_001039"
     CDS             complement(45523..46596)
                     /locus_tag="ACHW4Z_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR8880783.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46718..46879)
                     /locus_tag="ACHW4Z_001040"
     CDS             complement(46718..46879)
                     /locus_tag="ACHW4Z_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR8880784.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46996..47601)
                     /gene="osmY"
                     /locus_tag="ACHW4Z_001041"
     CDS             complement(46996..47601)
                     /gene="osmY"
                     /locus_tag="ACHW4Z_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR8880785.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47994..49583)
                     /gene="prfC"
                     /locus_tag="ACHW4Z_001042"
     CDS             complement(47994..49583)
                     /gene="prfC"
                     /locus_tag="ACHW4Z_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR8880786.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49674..50351)
                     /gene="yjjG"
                     /locus_tag="ACHW4Z_001043"
     CDS             complement(49674..50351)
                     /gene="yjjG"
                     /locus_tag="ACHW4Z_001043"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR8880787.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50366..50812)
                     /gene="rimI"
                     /locus_tag="ACHW4Z_001044"
     CDS             complement(50366..50812)
                     /gene="rimI"
                     /locus_tag="ACHW4Z_001044"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8880788.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50781..51194)
                     /gene="holD"
                     /locus_tag="ACHW4Z_001045"
     CDS             complement(50781..51194)
                     /gene="holD"
                     /locus_tag="ACHW4Z_001045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR8880789.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51297..52328
                     /gene="rsmC"
                     /locus_tag="ACHW4Z_001046"
     CDS             51297..52328
                     /gene="rsmC"
                     /locus_tag="ACHW4Z_001046"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR8880790.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52605..52691
                     /locus_tag="ACHW4Z_001047"
     tRNA            52605..52691
                     /locus_tag="ACHW4Z_001047"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52639..52641,aa:Leu,seq:cag)
     gene            52720..52806
                     /locus_tag="ACHW4Z_001048"
     tRNA            52720..52806
                     /locus_tag="ACHW4Z_001048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52754..52756,aa:Leu,seq:cag)
     gene            52841..52927
                     /locus_tag="ACHW4Z_001049"
     tRNA            52841..52927
                     /locus_tag="ACHW4Z_001049"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52875..52877,aa:Leu,seq:cag)
     gene            complement(52966..53202)
                     /locus_tag="ACHW4Z_001050"
     CDS             complement(52966..53202)
                     /locus_tag="ACHW4Z_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR8880791.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53343..54131
                     /gene="fhuF"
                     /locus_tag="ACHW4Z_001051"
     CDS             53343..54131
                     /gene="fhuF"
                     /locus_tag="ACHW4Z_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR8880792.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54169..54846)
                     /gene="bglJ"
                     /locus_tag="ACHW4Z_001052"
     CDS             complement(54169..54846)
                     /gene="bglJ"
                     /locus_tag="ACHW4Z_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR8880793.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(54804..>55370)
                     /locus_tag="ACHW4Z_001053"
     CDS             complement(54804..>55370)
                     /locus_tag="ACHW4Z_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8880794.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13252 a        14942 c        13817 g        13359 t
ORIGIN      
        1 aaattatcag tttcatcata taagatttga ctgcatccag ggcgagcaag gtgaattagt
       61 ttttgaaccc agtgacgttg aatattactt tgaaccagtg acgatacagg agtcaggaac
      121 cagcatatta aaaaatgagt tagaaaacag tgaggacggt gcgggtcaaa taggctttca
      181 actctccaat gatggtacga atgaaatcca gtatggtaaa agcaattact actcttttca
      241 gcatccccat gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa
      301 caatgtctca agcgggcaaa taatgagccg agtcaaaatc gtggtaatgt acaactaatc
      361 tatctccccc accttaactt tgggggagct gccgattaac gcaccgctgg actaaaaagc
      421 gcagaataat ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca
      481 aaaatagcat ggtactcttc catcacattc tcgacgcgac caatttctac gacagtttta
      541 tcggcataaa agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcatcgca
      601 ccaaaagctt tcatcaaagc ggcatcatca aactcgccga ggatttctac gtttaatccc
      661 tgggagttaa accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa
      721 agtcgccgtt cttccagaca agccgggaac ggtttttctg gtggtggatt tgtacaccag
      781 aaactcacgc cacattcgcc aattctcacg gagaacaggc cttcctgctg cgtagagtct
      841 atcggacagt cagaaatgat catatccagt ttatgctgac ttaattgctc cagcagcatt
      901 tcgtgggtgg attcgaagca gcgaagatga atgggctcgc cttctaccac tgcggcgtta
      961 agtacgctac tgaccaggcg tttggaaagt gcatcagcca cgccaacgtc aaacaataaa
     1021 ttggattctt tgcgatagtt cacaatatcc agcatttcct ggcttaaggt gaacatttta
     1081 tcggcatagc gatagaccag ttctcccagc tcgctgggtt cgagaccacg tcccttgcgt
     1141 ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt aatggtttgt
     1201 ggagttaaat aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag
     1261 aagtaataca agtggttgta attgatatga gacatgctca tttctctccc tgataacaat
     1321 gaaaagggag ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac
     1381 gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga
     1441 gtttcgccca gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga
     1501 tagacatagt aaaaccgata ccgcacagga tccccaccac cataatttgc tgataagtcg
     1561 ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc
     1621 ccagcggttt gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc
     1681 catccagcgt gacgccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa
     1741 tcagatacgc cacccacggg tgcaacacat gctccagtcg cttcgctgga gaacgcccat
     1801 gcttctcttt caaaggaata aagaagccga caattacccc cgccagagtt gcgtgaaccc
     1861 ccgatttcaa caccgcagtc cacaacacca cgccaacaag aatatagacg cccgtgcggc
     1921 gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaaggg
     1981 aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt
     2041 cgataatagc cagagccatc aaaaagatct tcagcactaa cggaacacga cttcccaaca
     2101 gcgccagtac accaagtgca aaagcaatgt cagtagccgc cgggatcgcc cacccttcgc
     2161 gggtaatcgg atcggcatag ttaaaagcca gatagagtaa tgccggcaca atcatcccac
     2221 caatagcggc gataactgga aatgcggcct ggcgtaagct ggctagcgat ccttgcatca
     2281 gttcacgttt aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcatcattta
     2341 tccataacag catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct
     2401 ccagaaagtc gtgataccat ccactggttg cgccgctgtt ggccataatc atcgccagga
     2461 tagcggcaat gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat
     2521 gtttcacttt ttatttctct ttcaggtgaa tagatcgttt ttactatttt accccgcacg
     2581 aatcatcgtt aaaatcagat tatagtgtag gaataattcg ttttttacga gccattgaag
     2641 aatttgcgcc atcagcggtg agatgcgttt ttgacggctg taagcgctca cgagtgaccc
     2701 taagcccaga taatcatagc ttaaggtgag gattcctgag ggattaatta ggaaaagtaa
     2761 taattaattt gttgtcagtc ggcgatgctt gttaggccaa tatttcaaaa ataatccagc
     2821 ttgtcgtcaa taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa
     2881 attacgccac aaaaatgcga ggcgtcttca ggattagaat cgataaaaca aaatgggtta
     2941 aagagagcga tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac
     3001 ggcagataga gaaaagcccc gagtgatatt ttaccatcaa cccgaggcct cctatatgct
     3061 gaacacatgt agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca
     3121 gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct
     3181 ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt
     3241 tttcacggct tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca
     3301 ggtgctcgca tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt
     3361 tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt
     3421 gagcgcgggc tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc
     3481 agctgtttct gcttttcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa
     3541 tcaccctgtg cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaatagctta
     3601 ccggtctggg tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg
     3661 atttcgccac ccagcgccgc catagcgaag ttgatcggga cttcgcaata caggttgttg
     3721 ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc
     3781 ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc
     3841 acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga
     3901 catttgttgc acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc
     3961 tgtacagcga agaatccctg gcgcatctgc acctgaccag aaccatgaca ggtcggacag
     4021 gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga
     4081 gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg
     4141 ttatagcgta aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata
     4201 tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa accaccgccg
     4261 cccatgcctc cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc
     4321 gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg
     4381 tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttctgatt
     4441 tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc
     4501 ttttttaaat tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt
     4561 cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc
     4621 gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg
     4681 ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg
     4741 cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag
     4801 cgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg
     4861 cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa
     4921 cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc
     4981 gttcagacca gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt
     5041 cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat
     5101 gccgcgcggt gccgggttga taccatctag gttgaactga cccagagatt tgttatcagc
     5161 cgcacgttta cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc
     5221 ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt
     5281 catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac
     5341 gtcttttacg tcaccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc
     5401 gtccgggtta acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac
     5461 cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag
     5521 gccagcatcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac
     5581 caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc
     5641 tgcagtgatg tatggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc
     5701 tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc
     5761 ctgatctttc ttgaattctt caaccagata gttgatcaga cggctgtcga agtcttcacc
     5821 acccaggtgg gtatcaccgt tggttgccag aacttcgaag gttttttcgc cgtcaacttc
     5881 gtcgatttcg ataatagaaa tatcgaaagt accacccccc aggtcataaa ccgcgatagt
     5941 acggttgcca gtgcctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat
     6001 gatacgtttt acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc
     6061 atcgttaaag tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc
     6121 ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa atctgcggcg gtgccatttt
     6181 ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat
     6241 gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat
     6301 cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag
     6361 agtttcacca tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt
     6421 ctccagcacg cgaggaatgg tgccatccat aatcgctaca caagagttgg tagtacccag
     6481 gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg
     6541 ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc
     6601 agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag
     6661 atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatggcagga caagacgtaa
     6721 aattgacagc aattttattc atataaagaa tgaatcgcag ccaaggcgtt catcaaaaaa
     6781 ttgtaataaa aagaaaagat tacgtgcctg aatcttctct ttatcagcag taaactagtg
     6841 ggtattcatc cccctacctc ttcccactaa gagaatcctt atgaaatccg tttttacgat
     6901 ttccgccagc ctggcgatta gcctgatgct gtgctgcacg gcgcaggcaa acgaccataa
     6961 actcctcggc gtcattgcaa tgccgcgtaa cgaaaccaac gatctggcgc tgaaacttcc
     7021 tgtttgtcgc attgtgaaac gcatacaact ctctgccgac catggcgatt tacagttaag
     7081 tggtgcatcg gtttatttca aagccgcccg tagcgccagt cagagcctga atattccttc
     7141 agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata acgacaataa
     7201 acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg aactcatcgg atatggccac
     7261 gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta
     7321 attacctgaa tgattcagat ctggattttc ttcagcattg tagtgaggaa cagttggcaa
     7381 atttcgcccg attgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcatactga
     7441 tgcgcaacga actgtttaaa tcgatggaag ggcatcccga gcaacatcgc cgcaactggc
     7501 agctgattgc cggagaatta cagcattttg gtggcgatag tatcgccaac aaactgcgcg
     7561 gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaagcgattg aagctgaaag
     7621 ccgacaaaga gatgtctacg tttgaaattg agcaacagtt actggaacaa tttctgcgta
     7681 atacctggaa gaaaatggac gaggaacata agcaggagtt tctgcacgcg gtcgatgcca
     7741 gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag
     7801 gtgtgtcgca tttgctttcc agccaactga cccgcatttt acgcacccac gcagcaatga
     7861 gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg aggccctgtg ggcgcagcat
     7921 taaatggggt taaagcggtc agcggcagca cctatcgcgt gacgattcca gccgtactgc
     7981 aaatcgcctg cctgcgccgg atggttagcg ccactcaggt ctgataccgg aaaccgggga
     8041 aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc
     8101 gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat
     8161 ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc
     8221 ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttatttcgc tctggacggt
     8281 attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg
     8341 gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg
     8401 ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca
     8461 cagttccttg gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc
     8521 actctgaaag gcgcgcgcgt cctgcaattc gttttcttta gcctgaccgt gctgtttgcc
     8581 ctgctggcga tcggtaacat tgccggtaat gccgcgatca tccactttgc cggctggatt
     8641 ggtctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtgct gaacgagcag
     8701 tttggtcgca ccgttctgcc gattggtgaa tcccactaat tgtcttgaga tccccccgct
     8761 cggggggatt tttttattcg ctaacgtcgc gtcttgcact cttcggtctg aatgcagcaa
     8821 ccacttctgg tgccgtttca acgtatggcc cttccagcaa ctgaatgcag tacggtacgc
     8881 tggcaaaaat accatgcacc acaacgttac cctcagcgtc cttcacacct tccagcgtct
     8941 ctttaataga cttcggctga ccgggtaagt taaggatcag cgcctgtttg cgaatcacgc
     9001 ccacctgacg cgaaaggatc gcagttggta caaaatgcag gctgatctgg cgcatctgtt
     9061 caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac
     9121 gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacca
     9181 gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt
     9241 caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgctggaatg cctttatcct
     9301 gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca
     9361 tcaagatttt tccgtttaaa caatctcgtt aaacagaatg atacactgcg aagggagtga
     9421 cagacaggct tcagaagagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca
     9481 gcagatcgcc gatcattttt tccagttttt cctggtcaat agcaaactta cggatacctt
     9541 ccgccagttt atctactgcc attggatcct ggttgtgctg ccacaggaac tcggactcag
     9601 tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc
     9661 cttcgctctc cgccagctct ttcagcagtg ccggtgcgat ggtcagacgg tcacagcctg
     9721 ccagttccag aatttcgccg atgttacgga agcttgcgcc cataaccacg gtttcataac
     9781 cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg
     9841 gagcgtactc tttcttatcg gtattcgctt tgtaccagtc aagaatacgg ccaacaaacg
     9901 gcgagatcag gaacacgccc gcctccgcac aagcacgagc ctgagcgaag gagaacagca
     9961 gggtcaggtt acagttgata ccttcttttt ccagctgttc tgcagcacgg ataccctgcc
    10021 aggtagaagc cagtttgatc agaatacgat cgttgctaat acctgcatcg ttgtagagtt
    10081 tgatcaggcg ttttgctttc gcaattgacg cttcagtgtc ataggaaaga cgcgcatcaa
    10141 cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata tttactgcca
    10201 gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg
    10261 cgacagcatc atcaatcaac ttacggtatt ccggaatctg cgctgcgtta agaatgagag
    10321 aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt
    10381 cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc
    10441 tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac
    10501 gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag actgtttcgg atttctgaca
    10561 ctgcgttgat atcgcccacc atttttatac aaaacctcat gtatgctacg cagaagttat
    10621 caagtacctc gtagagtata tacttcttaa acaattggta acgtttacac aggaaagtca
    10681 tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc
    10741 ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc
    10801 accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt
    10861 catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc
    10921 gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta ccgccggtgg accaggtgcc
    10981 gtcttttgga tgtgggttgc tgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc
    11041 cttgcacaac tttataaaga acgtgacgtc aatgggcagt ttcgtggcgg accggcatgg
    11101 tatatggcgc gcgggctggg gatgcgctgg atgggcgttc tgttcgccgt ctttttgctc
    11161 atcgcctacg gcataatttt cagcggagtt caggcgaacg ccgttgcccg agccctgagt
    11221 ttttcttttg attttcctcc gctggtgacg ggcattattc tcgctgtctt tgctctgctg
    11281 gcaatcactc gtggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg
    11341 gcgataatct gggtactgac cagcctggtg atttgcgtaa taaatatcgg gcaacttccc
    11401 cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg
    11461 gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat
    11521 gaggtgggaa tgggatcgac gccaaacgcg gcagcggcag ctgcatcctg gcctccgcat
    11581 ccggcagcac aagggattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt
    11641 acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat gccgctggaa
    11701 ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg gttcctaggg tgctgaattt
    11761 gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc
    11821 gaaaacaatc tcttcttttt acgcctgaac aaccctaaag cgatctggtg tttgcggatc
    11881 tgcaccttcg caacggtcat cggcggcacc ttgctaagcc ttccgctgat gtggcaactg
    11941 gcagatatca taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
    12001 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
    12061 gtgtttgatc cgttgcgtta tccggatatc gggcgccagc tttctccgga cgcgtgggat
    12121 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
    12181 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
    12241 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
    12301 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
    12361 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
    12421 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
    12481 caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
    12541 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
    12601 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
    12661 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
    12721 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
    12781 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
    12841 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
    12901 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
    12961 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
    13021 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
    13081 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
    13141 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
    13201 cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
    13261 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
    13321 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
    13381 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
    13441 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
    13501 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
    13561 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
    13621 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
    13681 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
    13741 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
    13801 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
    13861 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
    13921 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
    13981 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
    14041 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
    14101 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
    14161 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
    14221 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
    14281 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
    14341 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
    14401 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
    14461 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
    14521 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
    14581 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
    14641 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
    14701 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
    14761 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
    14821 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
    14881 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
    14941 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
    15001 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
    15061 agagcgaaca aggtcgggcc ggagccggag ataccgctcg ctaccgcgcc gatttccgcg
    15121 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
    15181 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
    15241 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
    15301 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
    15361 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
    15421 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
    15481 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
    15541 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
    15601 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
    15661 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
    15721 agtttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
    15781 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
    15841 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
    15901 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
    15961 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
    16021 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
    16081 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
    16141 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
    16201 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
    16261 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
    16321 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
    16381 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
    16441 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
    16501 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
    16561 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc
    16621 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
    16681 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
    16741 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
    16801 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
    16861 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
    16921 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
    16981 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
    17041 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
    17101 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
    17161 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
    17221 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
    17281 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
    17341 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
    17401 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
    17461 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
    17521 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
    17581 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
    17641 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
    17701 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
    17761 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
    17821 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
    17881 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
    17941 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
    18001 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
    18061 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
    18121 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
    18181 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
    18241 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
    18301 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
    18361 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
    18421 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
    18481 gttgtgtact ctgtaatttt tatctgtttg tgcgctatgc ctatattggt taaagtattt
    18541 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
    18601 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
    18661 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
    18721 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    18781 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt
    18841 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    18901 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    18961 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    19021 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    19081 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    19141 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    19201 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    19261 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    19321 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    19381 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    19441 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    19501 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    19561 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    19621 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    19681 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    19741 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    19801 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    19861 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    19921 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    19981 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    20041 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    20101 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    20161 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    20221 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    20281 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    20341 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    20401 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    20461 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
    20521 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    20581 cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    20641 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    20701 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    20761 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    20821 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtctat attacgagtc
    20881 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    20941 tcggcagagt tgagcagtcc ccacatcact ccatccagca acaacagcgc gagggtaaac
    21001 aacatgctat tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    21061 ccaatcagac tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc
    21121 aagagcaaat aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa
    21181 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga
    21241 atggcgtact tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg
    21301 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    21361 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc gctagcttcc
    21421 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    21481 atttcgctat tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    21541 gccatattca gctgcaggtt ctgcttccgc cagtcccctt cgggtaaagg gatatgcacg
    21601 ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg tcagtgccgt tccgttaact
    21661 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaagggc
    21721 ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac gcgaaacatc gaaatcggct
    21781 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    21841 acgttctgat tgccatcaac catcaatgac tcaggtaacc aaaaatggat aaaacttcgt
    21901 ttccgctcca cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg
    21961 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    22021 tcgctacggt aatcggcacg ttcgacaatc acttgccgaa tcagcattat cggaatcaac
    22081 aacagcaata ctgcaccaaa caaggtggtc attttccaga acagggggga tttcaacata
    22141 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    22201 gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    22261 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    22321 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    22381 tagtcaggaa taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact
    22441 tcggcgctta gcgttatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa
    22501 ttccccagcg cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcata
    22561 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    22621 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    22681 tggcgtagta acgtttccac cagcgcctgc attcgcgcat tttgtgtcag aatgttgtca
    22741 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    22801 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    22861 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg
    22921 ctactaccga gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga
    22981 gtgagccggg caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca
    23041 atccccaaca aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga
    23101 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgcccata
    23161 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    23221 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    23281 ccaacggctt tatttgccga atcgaacaat actttgccat gcgcatcggt catatagaca
    23341 tgatattcat tgcgcacttt gttaatgcca ccaatattgg cgcgaaacgg gcgatgttgt
    23401 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    23461 cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg tcccctccgt tgctcttcgc
    23521 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    23581 agtaaaaaat agcccagcag caaccgcatg ccgatacgca ttacaagccc ctcaggctat
    23641 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    23701 cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    23761 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    23821 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg
    23881 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gtcgagaact
    23941 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    24001 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    24061 gggccgtcag gaacagtaca ggtaacgccg gatagagcgc cagtaactgg cggcacaatt
    24121 caaagccact gatatcaggc aaaccaacgt cgagaatcat gacgtcggga acctgctggc
    24181 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    24241 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    24301 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    24361 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    24421 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    24481 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    24541 cgacttgctg acaagaaatg gccgcatcgg aggtatcttc tgccaggccc aatccccctt
    24601 taataccacc ggttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt
    24661 caaaggcttc cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg
    24721 aaccaatctc ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca
    24781 aatgcttgta tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac
    24841 ggtaaataac atattgatta agaacaaaat ctcaattctt ttatttccgt caagccctaa
    24901 aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca
    24961 attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg
    25021 cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga
    25081 caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt
    25141 cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc
    25201 actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca
    25261 acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg
    25321 ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat
    25381 gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta
    25441 ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca
    25501 cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga atgaaacgcg
    25561 tccgagtcag gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga
    25621 tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt
    25681 gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg
    25741 aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt caggatattg agcgatacta
    25801 cccggcagaa gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat
    25861 tcctatccgc cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc
    25921 ccctgcggtt tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg
    25981 cgaaatagaa cagttacgca gacgtaagcg gcgctctgcc catgctggta atccgagaat
    26041 cgtactcacc agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg
    26101 cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga
    26161 ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc
    26221 ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg
    26281 caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg
    26341 ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt
    26401 tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg
    26461 aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat
    26521 gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa
    26581 gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag
    26641 atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa
    26701 caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc
    26761 cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcattga tggcgacagc
    26821 actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact
    26881 ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg
    26941 agtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    27001 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    27061 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    27121 acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    27181 tcgcttttca gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg
    27241 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    27301 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    27361 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    27421 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    27481 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac
    27541 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    27601 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt
    27661 aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    27721 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    27781 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    27841 catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag
    27901 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    27961 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    28021 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    28081 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    28141 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    28201 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat
    28261 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc
    28321 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg
    28381 ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg atgcaaaatc tctttcgctt
    28441 cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct
    28501 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    28561 caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt
    28621 catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga
    28681 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca agcgacggga
    28741 tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca
    28801 tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt
    28861 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    28921 ccagcactgt taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat
    28981 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    29041 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    29101 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    29161 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    29221 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    29281 tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa
    29341 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc
    29401 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    29461 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    29521 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    29581 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    29641 actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg
    29701 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    29761 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    29821 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    29881 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    29941 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    30001 tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt
    30061 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    30121 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    30181 ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga
    30241 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    30301 cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga
    30361 cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg
    30421 ctggatcctc gaactggacc gcggtgaagg aattccgtgg gaaggtaact actcctcctg
    30481 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    30541 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    30601 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga
    30661 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    30721 caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc
    30781 gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    30841 tatgatctcc ggtcaggaac cgccggacag cggcaccatc actttgggtg aaacggtgaa
    30901 actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    30961 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    31021 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg
    31081 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    31141 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    31201 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    31261 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    31321 tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    31381 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct
    31441 tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg
    31501 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc
    31561 cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc
    31621 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc
    31681 attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc
    31741 cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg
    31801 aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc
    31861 accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg
    31921 atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct
    31981 tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta
    32041 ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtgac ccaacgcgat tttatcgtag
    32101 tcggaatact gcaatgcgat ttcatcaccg ccgaggtgtg aaaagacata atcgcggcca
    32161 tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta
    32221 ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta
    32281 ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata
    32341 aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc
    32401 gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac
    32461 tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg
    32521 ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga
    32581 cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg
    32641 gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata
    32701 aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc
    32761 ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg
    32821 gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca
    32881 tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat
    32941 gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg
    33001 agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg
    33061 ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac
    33121 tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg
    33181 gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcggcgctgg
    33241 tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca
    33301 gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca
    33361 ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca
    33421 gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc
    33481 cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg
    33541 cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa
    33601 aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt
    33661 tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc
    33721 gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag
    33781 gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg
    33841 gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga
    33901 gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt
    33961 cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca
    34021 gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc
    34081 cgcctcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa
    34141 ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga
    34201 gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc
    34261 aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac
    34321 aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg
    34381 caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc
    34441 ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat
    34501 ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa
    34561 tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg
    34621 ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga
    34681 tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa
    34741 agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca
    34801 ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg
    34861 cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc
    34921 cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc agcttggcaa
    34981 tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg
    35041 gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca
    35101 ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc
    35161 accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct
    35221 gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg
    35281 gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag
    35341 gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga
    35401 aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt
    35461 caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt
    35521 cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa
    35581 ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt
    35641 gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc
    35701 gatcctcgat cagttaacag atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca
    35761 aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga
    35821 cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa
    35881 cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca
    35941 acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg
    36001 cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt
    36061 cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc
    36121 cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta
    36181 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
    36241 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
    36301 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
    36361 tggcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg
    36421 caagccggag tacgataaaa ctatctccac gtcgattgtg ctcaatgcgc tgaacgcgct
    36481 cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga
    36541 ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac
    36601 cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa
    36661 actggcggcg aaaggcatta cttcagtgcg ttcccgcgtg accaacctca ccgagctgct
    36721 gccggggctc accatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
    36781 ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    36841 gccgaaacct ttgcccgtca gagtagctga gagtggaact tcggtcaggc tccggcattc
    36901 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    36961 aaaggccata tcacccgcgc ccaggtattt accgacagcc tcaaccccgc gccgctggaa
    37021 gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc
    37081 gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg
    37141 atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga
    37201 tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct
    37261 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    37321 ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    37381 caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    37441 ggctgaacat aaatgaatag attcgtgaat agatacttga atgtttttta ttttctatcc
    37501 atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag
    37561 ccggagcagt cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac
    37621 tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc
    37681 tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg
    37741 gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga taccagccgc ttccatttcc
    37801 acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac
    37861 aggtcagcgg agaacaggtt acccacgcga gcatcaacgc ccagtgcttt agctgcatct
    37921 actgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatggtc tttaaaacgg
    37981 atgcggttaa ctttggaatc ggtgcaggcc cccataccga taacgacgtc gcgcagtttt
    38041 acgtgcggca gaactgcgcc acaggaacca acgcggataa ttttcttcac gccgaaatcg
    38101 gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg
    38161 gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac gttgttcact
    38221 tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc
    38281 atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt
    38341 ttatcctttt tgtgacataa caaaggcgga gtcaacgctc cgcccaaatc catcagaaca
    38401 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    38461 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggcca tataccagta
    38521 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcgcaa ccgtggtcag
    38581 cggtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    38641 cgaacagttc cagacccgca gcataaccgg cgacgtcgcg gcggtggccc caggaagagt
    38701 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    38761 tggtggcgtc aaacagcgcg tccaagccag tcgctttcac ttttttggtg ataccgcagt
    38821 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg gccgtgtttt tcatcaacca
    38881 gtttctgcag cacggtcggt gctggcggct caacagccaa gtcgtgacgg ttaccggtac
    38941 gctggaagtt accagctttg tcaccgataa acggacgagc gataacacga ccgatattgt
    39001 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac
    39061 cgaaagtttc ttcatggcag gcaatctgga acacggagtc agcggaggta tagaaaatcg
    39121 gcttgccggt tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt
    39181 ggcagttacc gaggtaaccc ggcagattag cgcgttcgac cagtttatcc agcagctctt
    39241 gcgggaagct gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg
    39301 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    39361 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    39421 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcggc cctttacgac
    39481 cattatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    39541 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    39601 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    39661 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gcgttatcgg
    39721 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    39781 cgaggataac cgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag
    39841 taaagccgac gctgtaatcg atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    39901 ctactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    39961 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    40021 tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta ccgttgtcca
    40081 gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagatca
    40141 acatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    40201 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    40261 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    40321 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    40381 tcacgtccat caccagcgcg tccagacctt ccgccagttt cttcgccagg atcgaggcag
    40441 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    40501 cagccggagc cagtgaactg gtctgaccga taatcgccac gccgacgtct ttaataattt
    40561 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    40621 gcgtaccgcc agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg
    40681 ctgcgaccat cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt
    40741 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    40801 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    40861 tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc gcgaataccg ttgataaaga
    40921 aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg
    40981 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    41041 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    41101 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcgccgtgg cccagcgctt
    41161 tcagcaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tcagcccagt
    41221 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    41281 cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    41341 tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    41401 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    41461 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    41521 tacaggcttt caccaggtca aaaccaacct gctcgtcacc cgccatcagc gcgcggtacg
    41581 ggaacaccac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    41641 cgatttcgat gtcgtcgtta ccgtgtggga agttggttac cgtagcgata cggatttccg
    41701 gggtgccctg ctctttcaga gttttgcgag caatcgggat aaaacgagga tagatacaga
    41761 tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt actttctcgt
    41821 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    41881 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    41941 tgttagtatt ctaacatcta ctccgcaaca cacttcgata cacatcacaa ttaaggaaat
    42001 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    42061 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    42121 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    42181 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    42241 tttctggcac tggaagcaga aaacaatctg ccttacccac agcttcctgc cgaagcccgc
    42301 cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    42361 cgctatgtct tacccgatta cgctcgtttt ctggcgaacg gttccgaatg gctggagctg
    42421 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    42481 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccgtat
    42541 gttagaattc taacacaaaa cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    42601 gacagaaccc tgccagatgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    42661 acccgcgcga tcttacgtgc agacgcgcag gtttcaccga acctgacctt tatctacgat
    42721 cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc
    42781 aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg
    42841 attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt
    42901 aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta
    42961 ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa
    43021 caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt
    43081 tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgagaaa
    43141 gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg ttatcgcatc
    43201 agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc
    43261 gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt
    43321 ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat
    43381 gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaac
    43441 ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc
    43501 gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat
    43561 ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc
    43621 gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg
    43681 cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc
    43741 tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg
    43801 acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt
    43861 tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagat
    43921 gtgtccgcaa catgccacac caatggcgca atccatgagc gtggacgaag tgcttagcca
    43981 tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac
    44041 ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca
    44101 tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg aaaaattact
    44161 cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat gtcatcaaca
    44221 attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg cagagcgcgg
    44281 caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt tgcaacacat
    44341 cgaagaactg gcggcgttta tcaagggact tggtgatgtt ccggttcgcc tgaacgcgtt
    44401 tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt
    44461 tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct
    44521 ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg cctgataaga
    44581 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
    44641 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag cgtcgcatca
    44701 ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca cgaacgttaa
    44761 acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt tccgggcgta
    44821 actcgcaaag cacggcaaac acacgggcag cctgttccgg gcggttaggc tgcccctgaa
    44881 aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc agcggtaatt
    44941 ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg cctacgccga
    45001 ttttgtagcc cagctgtaca aaccgttcag cctgttgctg gctgccagaa aatccgtgga
    45061 caacaccagc acgcggcaaa tcgtggcgtt taagatgcat cgccagtttg tcgtgcgtgc
    45121 gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt tgttcgtcga
    45181 gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga ccgatctccc
    45241 ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctgc tcaagagaca
    45301 catcgctatg tttttccaac ataccaggat gcaagcccaa tacggcatac agcggttgat
    45361 aattttccgc taacgcctgc acacgggcaa aattttccgc ctctgtcgcc ggaacaatga
    45421 tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg ccaataaaag
    45481 gcgggaaatc aaaatggcag tgagtgtcga taaaacggca aatcaagcca gatcctcatt
    45541 gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact tcggcaacca gcgtatcgtt
    45601 ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca ccagatggcg
    45661 ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat aacgaccgca
    45721 taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa gcgcgatact
    45781 atgtaatggc ttcggcggat aaatttcgga tatccgcagc ttgcccggtg gtttctcaat
    45841 aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga ccagcggctg
    45901 caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg tgtagtacat
    45961 ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg ccgcctcttt
    46021 aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca gcgacactcc
    46081 gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc agttttgttt
    46141 ggttggtaaa aagtaattcg gcgcgtagtc atcctgacga caggcgcaca tataaaacga
    46201 tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct ggcttgcggt
    46261 ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc gcaatggatc
    46321 gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac cgggctgatt
    46381 gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa gatcgaaagg
    46441 attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc cacgctgtcc
    46501 gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg acaacggcgc
    46561 aatattacca agcgttacag gtattcgctg ccccacttct gccttcctgt ttttgttgtg
    46621 aaagaaagac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc agtccgctgg
    46681 actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt cggcccatga
    46741 acaaactcac taggaacaga ataatcccga cgacaaagac aattttagct gcgcctgcag
    46801 cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata accagaaata
    46861 tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc gcttgatatg
    46921 agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc tcccgacaaa
    46981 gttcgggagg acgaattact tagttttcag atcattttta acgcttttca caccatctac
    47041 cgctttggcg atactttcag cacggtcact ttgtgcctga gaatctacgg taccggagag
    47101 ctgaaccacg ccatcggtgg tttcaacttt cactttacgt gaagggacga tatcgtccgc
    47161 cagcagtttg gctttgattt cactggtggt ggcggtgtca cccgcgtagc ccttcaccga
    47221 gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca gaggtcacgc cttcaacgcc
    47281 tttcgccact ttcactgcct cttcggcctg ggcctgactt tcaacgaaac cgctcagggt
    47341 cacgactttt tgatcggttt tgacagagat atcggtgctc ttgatgttgt catgatccac
    47401 cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc atgaaattac cgactttatt
    47461 catagagcta tcgacttttt gccctgcgct ttcattggta gtctgcgcgt tgttttccgc
    47521 gtaggcagag ccggtcgcga cggcagaggt caacattaca gccagcagag ttttcgaaat
    47581 cttcagtctt gtcatagtca tcgatttatt cctgtatgtt tgctcgtaat ttgagcctag
    47641 caacacgagg ttgcattgct gaatagggag agacttcacc ctctacagaa gtcaatggtc
    47701 gccatcacaa aagcgatggg tgatgaataa cgaccattac agcctctgaa tcagttatta
    47761 atatcggtag aatgacaatc gacggcttta gatactaata tctacgcatt gaacggtatt
    47821 taacgccatc agaaatgtca tcactttgtt aaatatagat cacaattttg aaaccgctcg
    47881 ggatatcagc gaaaacataa gcaaaagtga atgttttaag aacattccgt aagcggctaa
    47941 taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc
    48001 tcgcgggtct ggtggaactg aacgtccgga taacgttcct gtgccaggcg caggttgacc
    48061 atgctggtag cgatgtaagc gaggttatcg ccgccatcaa gcgccagttg gctttcgttc
    48121 ttacgcttga actcttcgaa tttcttcgcg tctgcacatt ctacccagcg ggcagtggca
    48181 acgttgactg actcatacac tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc
    48241 acatcaaact gcagcacacc aaccgcacca acgatcagat cgttgttgga gattggacgg
    48301 aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc
    48361 ttcagcggat ctttcaggcg gatacgacgg aacagttctg gtgcgaagtt cggaataccg
    48421 gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg
    48481 tgcaggccga ggatatcgcc cggatacgct tcttcaacgt gcgaacggtc acccgccata
    48541 aaggtcagcg cgtcggagat caccacatct ttcgcagtgc gcacctggcg cagtttcatg
    48601 cctttttcat atttaccgga caccacacgc ataaacgcca cgcggtcgcg gtgtttcgga
    48661 tccatgttgg cctgaatttt aaatacgaag ccggtaaatt tatcttcgct cgcttctacg
    48721 gtacgggtat cggtctgacg cggcatcggc gcaggcgccc actccaccag gccattcagc
    48781 atatgatcaa cgccgaagtt acccagcgca gtaccgaaga ataccggagt gatttcgccc
    48841 gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt taaccagttc cagttcgtca
    48901 cgcagctgct gtgccagatc ttcaccaacc gcagcatcga gatccgggtt attcagccct
    48961 ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc tctgatagag ataggtttca
    49021 tctttataaa ggtggtaaac gcctttaaac agcttgccgc agccaattgg ccaagtgatc
    49081 ggtgcgcagc caattttcag ctcgttctca acttcatcga gcagttccat cgggtcgcgg
    49141 atatcacggt caagtttgtt cataaaggtg aggatcggcg tgtcgcgcag acgggtaact
    49201 tccatcagct tacgggtacg atcttcaaca ccttttgcgg cgtcgataac catcaggcag
    49261 cagtccaccg ccgtcagggt acgataggta tcttccgaga agtcttcgtg ccccggggtg
    49321 tcgagcaggt taaccaggca atcgtgatac ggaaactgca tcacagacgt agtaatggag
    49381 atcccacgct gcttttccat ctccatccag tccgacttag cgtgctggtt ggaaccacgg
    49441 ccttttactg taccggcggt ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg
    49501 gtagttttac cggcgtccgg gtgagaaata atggcaaaag tgcggcgctt cgccacctct
    49561 ttcaaataag gagacaacgt cataattctt tcttcttgag taagcggcat cgcgccgcgc
    49621 ttgttgaaaa cgaaaaattg cggctatttt acccatcaac gggggggagg caatcagtgt
    49681 ttacacagga gctgctccag ttcgtgcaac gaagaaacgg tccaggtggg cgcgatgcct
    49741 tctggttgct cgcgattgtg cgcattcagc cagcaggtcg caagcccggc gttgatgcca
    49801 ccgagaatat cggactcggc agtgtcgcca accatcagca cgcgtgaacg gtcaggattg
    49861 cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg gtttggcaac gccaacttct
    49921 tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg tccgttccag acgcacttgc
    49981 tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag cgttgctacg aatggcgtta
    50041 agcagagaaa ccgcgcccgg caacggcgta cagatttccg ccatcgcatt aataaaggca
    50101 tcgttaagca agcctgcttc aactttcagc cgttcggccc agctctcgaa acgcccgtgc
    50161 tgaagctgta atgaagtgat cgcgccgttt tgataatcca cccacagtgg cttgttaacg
    50221 gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc tgtaatcaag aaacatccgc
    50281 tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat cggcatcaaa gaaaatccag
    50341 tcccacttca ttattccacc ttgtcttaca tactgattgg caacgccatg atgatggcgt
    50401 cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat cgtcgcctcg ttaaagccta
    50461 aactttcgta cagggcaatg gcggcagcat ttgaggcacg gacttccagc catagtgtcg
    50521 ccacgccgcg tttttccagt tcgtcgatca gatgttccag cagcgcccta cccagcccct
    50581 gacgctgata gtcgggatcg accgcaatat tgaacaatgt agcttcatcc agcaccactt
    50641 gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa ctgaaagttg agataacgct
    50701 cgccctggtt gctggcaaag gttttttcac tccacggaaa ggcgtgggcg cgttgttcaa
    50761 tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga aatcgtgttc atatgtgcaa
    50821 atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc gtaattcggt gagcgccggt
    50881 gatgccacct gagcgccttc caatgatagc ggttcgtcag tacccaaccg ccagctgttg
    50941 cagcgactgc cttgcggcag catcgcgatt ttttctggcg tcagttgcag cacttgatcg
    51001 gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag gatcagtcag ggcgggaaga
    51061 tcgtttgcca ccatcaccag acggacgtgt gccgggctcg caatggcaat ctccccctgc
    51121 aacgcgccag ggcgacgcag cgaccactgg gtaatgccca gttgctgtaa ctgccagtct
    51181 cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc gcaaatatag caaattcgtc
    51241 gataccgcgc caacatatgg ctataatcgc cgccagtatc aattgaggag cattccatgt
    51301 ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag tgatgatttc gaacaaagcc
    51361 gtattctgtt tgccggagac ttacaggatg acctgcccgc gcgtttagat accgcggcca
    51421 gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt aagctgccag atgggggata
    51481 acgcccgttt tagtctggtc gccacggcga atgatgtcgc agattgcgat acgctgattt
    51541 actactggcc gaagaacaaa ccggaagccc agttccagtt gatgaattta ctttctctgc
    51601 tgccagtggg gacagatatt tttgtcgttg gcgagaaccg cagcggcgtg cgcagcgctg
    51661 agcagatgct ggcagattat gcgccgttga ataaagtcga cagcgctcgt cgctgtggcc
    51721 tctattttgg tcgtctggaa aaacagccag tatttgatgc ggataaattc tggggcgaat
    51781 acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt gtttagccgc gacggtctgg
    51841 atgtcggtag ccagttgctg ctctcgacgt taactccgca cacgaaaggt aaagtgctgg
    51901 atgtcggctg tggcgcgggt gtactttcag ttgcctttgc gcgccattcg ccgaaaattc
    51961 gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc cagccgcgca acacttgcag
    52021 ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt ttccgaggtg aaaggttgtt
    52081 ttgatatgat catctccaac ccgccgttcc acgatgggat gcaaaccagc ctggatgcgg
    52141 cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag cggcggcgag ctgcgaattg
    52201 tagcgaacgc cttcctgcct tacccggacg tgctggatga gacatttggc ttccatgaag
    52261 ttatcgcgca aaccgggcgc ttcaaggtat atcgcgccat tatgacccgc caggcgaaga
    52321 aaggttgagt atctgatatc tgtcgccgca taccgtgtcg gatgcggcgt gaacgcctta
    52381 tccgacctac aaaacctgca cgttagccct tcgtaggcca gataagacgc gccagcgtcg
    52441 catctggcat ctgcccccgc atgcctacca aacctgcacg ctagtttcct gatggacatt
    52501 tttccagcaa ttacacatca ggcgataatt aactattgac gaaaagctga aaaccactag
    52561 aatgcgcctc cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat
    52621 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    52681 ccctcgcacc aaaaatcacg ttgatattgc tcgcactggg cgaaggtggc ggaattggta
    52741 gacgcgctag cttcaggtgt tagtgttctt acggacgtgg gggttcaagt cccccccctc
    52801 gcaccaaacg aggcgatatc aaaaaaagta agatgactgt gcgaaggtgg cggaattggt
    52861 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
    52921 cgcaccaatt atctttactt cctttcttgt ttcttccctg ttttgtcatc cctgattcaa
    52981 attcattatc atcagcatga tgccagcaat aagcgccagg cacgatggta gcaaaatgta
    53041 atgccgcaac tgtttgtgat acagcatcac cggcgtcaat actaatccca tcagaatgac
    53101 aatccgccac tgatccggcg ataaatccgc atacatcagc cctgcaatca gcaatcccgg
    53161 cagcaacact ccccagccac tgcccagcgt acgttgcaac atggtttcat ctctttcatt
    53221 gataatgaaa accaatatca tatgataatt tttatcattt gcaagccaga gaaatccctt
    53281 gctatcgagt aaacctatcg ctaggattag caatcattat catttagatt actatcacga
    53341 ttatggccta tcgttccgca ccgctctatg aagatgtcat ctggcgaacg catctccagc
    53401 cgcaggatgc tgggcttgca caagccgtac gggcgatgat cgccaaacat cgtgaacatt
    53461 tgctggagtt tatccgcctg gatgaacccg ctccgcttaa cgccatgacg ctggcgcaat
    53521 ggtcatcacc gaatgcgcta agttctctac tggcggtcta ttccgatcat atctatcgca
    53581 accaaccaac gatgatccgc gagaacaaac cgctgatctc actatgggca caatggtata
    53641 tcggcctgat ggtgccacca ttaatgctgg cgctactgac gcaggaaaaa gcattagatg
    53701 tgtcgccgga acatttccat gctgagtttc acgaaaccgg acgcgtcgcc tgtttctggg
    53761 tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc gcagcagcga atggaaacgt
    53821 taatcagcca ggcgctggtt ccggttgtgc aagcactaga agcgaccgga gagatcaacg
    53881 gcaaacttat ctggagtaat accggttatt tgatcaactg gtatctcact gagatgaagc
    53941 aactgctcgg cgaggctact gttgaatcgc tgcgccatgc cctctttttt gagaaaacgc
    54001 tcacgaacgg tgaagataat ccactctggc gtaccgtggt actgcgcgac ggcctgctgg
    54061 tgcgccgcac ttgctgccag cgttatcgct taccggatgt gcagcaatgt ggctattgta
    54121 cgctgaaatg aaagcactgc cggggaagta aacccggcat catgcggatt aatagggatg
    54181 caacacatta cttgcttcgc aatgccgcat acataacaga atatcttcgg cctccaacaa
    54241 ccctgcatca gaactgacgc ccagtttcga catcacatta aatttatgcg ctcggatcgt
    54301 tttgatatta cgtttaagct gctcggcaat ttgtgtcatt gagtagccac gcgacataaa
    54361 gcgcaatatt tctctctccg tcgggcttaa cgtccggctt tggttaatgt accactgatt
    54421 gttcagtcgg tcggtcgcct gacgtacacc atttaatgac aaaaagagtt cctgatgaaa
    54481 aatctccagc gttgacgctt tacttaatac accgtccagc ggtgatggcg acaatgaacc
    54541 aatcagccga gcttcaatat catcatccac aataactaaa cgccgggtac gcggaaactt
    54601 aatcgccagc tcagtcaggc aagataatcc ctctctgcgc tcacttctca tggcagaaaa
    54661 agaaaaaatg accgccgcaa acgagatccg cgacatggca tcctgaaatg actcctgact
    54721 ggtataggta tggagcttat aagggttgcc cgcaaacttt ctgaataaac tctcaatacc
    54781 gatactactc atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg
    54841 ttccattctg cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg
    54901 ctatttatcc atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg
    54961 tttttttggg cgctgatagt tttattactt ttcttaagca atgaagctat ctggttgatg
    55021 ccccatcctt tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag
    55081 cgatagctaa aatcatgagt ttcagtcatt gccggagagg tcagcatggc gctgatacgc
    55141 tctgcgtgcg tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca
    55201 gacaataacg tcgccgtagg gcacatgagc agttctacag cctgggaata ccaggagcgt
    55261 gacaccatga aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca
    55321 caaacagaac gtggatcttc actttcaccg gctaaatcag caatgactaa
//
LOCUS       DAZSNG010000017        49830 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZSNG010000017 DAZSNG010000000
VERSION     DAZSNG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.017"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACHW4Z_001054"
     CDS             <1..1224
                     /locus_tag="ACHW4Z_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8880795.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHW4Z_001055"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHW4Z_001055"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8880796.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHW4Z_001056"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHW4Z_001056"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR8880797.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHW4Z_001057"
     CDS             2714..3427
                     /locus_tag="ACHW4Z_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR8880798.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHW4Z_001058"
     CDS             complement(3399..4076)
                     /locus_tag="ACHW4Z_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8880799.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHW4Z_001059"
     CDS             complement(4070..4747)
                     /locus_tag="ACHW4Z_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8880800.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHW4Z_001060"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHW4Z_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHW4Z_001061"
     CDS             complement(5443..6021)
                     /locus_tag="ACHW4Z_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR8880801.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHW4Z_001062"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHW4Z_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHW4Z_001063"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHW4Z_001063"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR8880802.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHW4Z_001064"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHW4Z_001064"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR8880803.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHW4Z_001065"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHW4Z_001065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR8880804.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHW4Z_001066"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHW4Z_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR8880805.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHW4Z_001067"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHW4Z_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR8880806.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHW4Z_001068"
     CDS             12291..13031
                     /locus_tag="ACHW4Z_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR8880807.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHW4Z_001069"
     CDS             13198..14148
                     /locus_tag="ACHW4Z_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8880808.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHW4Z_001070"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHW4Z_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHW4Z_001071"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHW4Z_001071"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR8880809.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHW4Z_001072"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHW4Z_001072"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR8880810.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHW4Z_001073"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHW4Z_001073"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR8880811.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHW4Z_001074"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHW4Z_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR8880812.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHW4Z_001075"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHW4Z_001075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR8880813.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHW4Z_001076"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHW4Z_001076"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR8880814.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHW4Z_001077"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHW4Z_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR8880815.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHW4Z_001078"
     CDS             23768..23827
                     /locus_tag="ACHW4Z_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8880816.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHW4Z_001079"
     CDS             complement(23928..24530)
                     /locus_tag="ACHW4Z_001079"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR8880817.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHW4Z_001080"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHW4Z_001080"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHW4Z_001081"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHW4Z_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR8880818.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHW4Z_001082"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHW4Z_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR8880819.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHW4Z_001083"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHW4Z_001083"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR8880820.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHW4Z_001084"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHW4Z_001084"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR8880821.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHW4Z_001085"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHW4Z_001085"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR8880822.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACHW4Z_001086"
     CDS             complement(29676..29759)
                     /locus_tag="ACHW4Z_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880823.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHW4Z_001087"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHW4Z_001087"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR8880824.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHW4Z_001088"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHW4Z_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR8880825.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHW4Z_001089"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHW4Z_001089"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR8880826.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="ACHW4Z_001090"
     CDS             34816..35559
                     /locus_tag="ACHW4Z_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8880827.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHW4Z_001091"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHW4Z_001091"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR8880828.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHW4Z_001092"
                     /pseudo
     CDS             37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHW4Z_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37568..38227
                     /locus_tag="ACHW4Z_001093"
     CDS             37568..38227
                     /locus_tag="ACHW4Z_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR8880829.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHW4Z_001094"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHW4Z_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR8880830.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHW4Z_001095"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHW4Z_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR8880831.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="ACHW4Z_001096"
     CDS             39893..40300
                     /locus_tag="ACHW4Z_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR8880832.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHW4Z_001097"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHW4Z_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR8880833.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="ACHW4Z_001098"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="ACHW4Z_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR8880834.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHW4Z_001099"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHW4Z_001099"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR8880835.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="ACHW4Z_001100"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="ACHW4Z_001100"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR8880836.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="ACHW4Z_001101"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="ACHW4Z_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR8880837.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="ACHW4Z_001102"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="ACHW4Z_001102"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR8880838.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHW4Z_001103"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHW4Z_001103"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR8880839.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="ACHW4Z_001104"
     CDS             47705..47776
                     /locus_tag="ACHW4Z_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880840.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHW4Z_001105"
     CDS             complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHW4Z_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR8880841.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHW4Z_001106"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHW4Z_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR8880842.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12451 c        13406 g        11922 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caagcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattgattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DAZSNG010000018        49737 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZSNG010000018 DAZSNG010000000
VERSION     DAZSNG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49737
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.018"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..185
                     /locus_tag="ACHW4Z_001107"
     CDS             <1..185
                     /locus_tag="ACHW4Z_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8880843.1"
                     /translation="SRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVE
                     LYDKVIGHYLNIKHYQ"
     gene            complement(179..1357)
                     /gene="setB"
                     /locus_tag="ACHW4Z_001108"
     CDS             complement(179..1357)
                     /gene="setB"
                     /locus_tag="ACHW4Z_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR8880844.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1725..2855
                     /gene="fruB"
                     /locus_tag="ACHW4Z_001109"
     CDS             1725..2855
                     /gene="fruB"
                     /locus_tag="ACHW4Z_001109"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR8880845.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2855..3793
                     /gene="fruK"
                     /locus_tag="ACHW4Z_001110"
     CDS             2855..3793
                     /gene="fruK"
                     /locus_tag="ACHW4Z_001110"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR8880846.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3810..5501
                     /gene="fruA"
                     /locus_tag="ACHW4Z_001111"
     CDS             3810..5501
                     /gene="fruA"
                     /locus_tag="ACHW4Z_001111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR8880847.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5925..6866
                     /locus_tag="ACHW4Z_001112"
     CDS             5925..6866
                     /locus_tag="ACHW4Z_001112"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR8880848.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6854..7792
                     /gene="psuG"
                     /locus_tag="ACHW4Z_001113"
     CDS             6854..7792
                     /gene="psuG"
                     /locus_tag="ACHW4Z_001113"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR8880849.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7886..9136
                     /locus_tag="ACHW4Z_001114"
     CDS             7886..9136
                     /locus_tag="ACHW4Z_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8880850.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9207..9901)
                     /locus_tag="ACHW4Z_001115"
                     /pseudo
     CDS             complement(9207..9901)
                     /locus_tag="ACHW4Z_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            10032..10973
                     /gene="rihB"
                     /locus_tag="ACHW4Z_001116"
     CDS             10032..10973
                     /gene="rihB"
                     /locus_tag="ACHW4Z_001116"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR8880851.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            11073..12323
                     /locus_tag="ACHW4Z_001117"
     CDS             11073..12323
                     /locus_tag="ACHW4Z_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8880852.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12379..13467)
                     /locus_tag="ACHW4Z_001118"
     CDS             complement(12379..13467)
                     /locus_tag="ACHW4Z_001118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8880853.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13470..14327)
                     /gene="nfo"
                     /locus_tag="ACHW4Z_001119"
     CDS             complement(13470..14327)
                     /gene="nfo"
                     /locus_tag="ACHW4Z_001119"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR8880854.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14401..15450)
                     /locus_tag="ACHW4Z_001120"
     CDS             complement(14401..15450)
                     /locus_tag="ACHW4Z_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR8880855.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15549..16430
                     /gene="yieE"
                     /locus_tag="ACHW4Z_001121"
     CDS             15549..16430
                     /gene="yieE"
                     /locus_tag="ACHW4Z_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR8880856.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16636..18105
                     /gene="lysP"
                     /locus_tag="ACHW4Z_001122"
     CDS             16636..18105
                     /gene="lysP"
                     /locus_tag="ACHW4Z_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR8880857.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18397..20388
                     /gene="cirA"
                     /locus_tag="ACHW4Z_001123"
                     /pseudo
     CDS             18397..20388
                     /gene="cirA"
                     /locus_tag="ACHW4Z_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20420..21255)
                     /gene="yeiG"
                     /locus_tag="ACHW4Z_001124"
                     /pseudo
     CDS             complement(20420..21255)
                     /gene="yeiG"
                     /locus_tag="ACHW4Z_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21513..22181
                     /gene="folE"
                     /locus_tag="ACHW4Z_001125"
     CDS             21513..22181
                     /gene="folE"
                     /locus_tag="ACHW4Z_001125"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR8880858.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22198..23274
                     /gene="yeiB"
                     /locus_tag="ACHW4Z_001126"
     CDS             22198..23274
                     /gene="yeiB"
                     /locus_tag="ACHW4Z_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR8880859.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23416..24456
                     /gene="galS"
                     /locus_tag="ACHW4Z_001127"
     CDS             23416..24456
                     /gene="galS"
                     /locus_tag="ACHW4Z_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR8880860.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24736..25734
                     /gene="mglB"
                     /locus_tag="ACHW4Z_001128"
     CDS             24736..25734
                     /gene="mglB"
                     /locus_tag="ACHW4Z_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR8880861.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25795..27315
                     /gene="mglA"
                     /locus_tag="ACHW4Z_001129"
     CDS             25795..27315
                     /gene="mglA"
                     /locus_tag="ACHW4Z_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR8880862.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27331..28341
                     /gene="mglC"
                     /locus_tag="ACHW4Z_001130"
     CDS             27331..28341
                     /gene="mglC"
                     /locus_tag="ACHW4Z_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR8880863.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28377..29590)
                     /gene="preA"
                     /locus_tag="ACHW4Z_001131"
                     /pseudo
     CDS             complement(28377..29590)
                     /gene="preA"
                     /locus_tag="ACHW4Z_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29584..30822)
                     /gene="preT"
                     /locus_tag="ACHW4Z_001132"
     CDS             complement(29584..30822)
                     /gene="preT"
                     /locus_tag="ACHW4Z_001132"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR8880864.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(31016..31255)
                     /locus_tag="ACHW4Z_001133"
     CDS             complement(31016..31255)
                     /locus_tag="ACHW4Z_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR8880865.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31258..31977)
                     /gene="sanA"
                     /locus_tag="ACHW4Z_001134"
     CDS             complement(31258..31977)
                     /gene="sanA"
                     /locus_tag="ACHW4Z_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR8880866.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32127..33011)
                     /gene="cdd"
                     /locus_tag="ACHW4Z_001135"
     CDS             complement(32127..33011)
                     /gene="cdd"
                     /locus_tag="ACHW4Z_001135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR8880867.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33141..33836)
                     /locus_tag="ACHW4Z_001136"
     CDS             complement(33141..33836)
                     /locus_tag="ACHW4Z_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR8880868.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33833..34231)
                     /locus_tag="ACHW4Z_001137"
     CDS             complement(33833..34231)
                     /locus_tag="ACHW4Z_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR8880869.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34470..35417
                     /gene="dusC"
                     /locus_tag="ACHW4Z_001138"
     CDS             34470..35417
                     /gene="dusC"
                     /locus_tag="ACHW4Z_001138"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR8880870.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35788..35871)
                     /gene="yohP"
                     /locus_tag="ACHW4Z_001139"
     CDS             complement(35788..35871)
                     /gene="yohP"
                     /locus_tag="ACHW4Z_001139"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR8880871.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36095..37531
                     /gene="mdtQ"
                     /locus_tag="ACHW4Z_001140"
                     /pseudo
     CDS             36095..37531
                     /gene="mdtQ"
                     /locus_tag="ACHW4Z_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37623..38345
                     /locus_tag="ACHW4Z_001141"
                     /pseudo
     CDS             37623..38345
                     /locus_tag="ACHW4Z_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38462..39040)
                     /locus_tag="ACHW4Z_001142"
     CDS             complement(38462..39040)
                     /locus_tag="ACHW4Z_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8880872.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39210..39797
                     /locus_tag="ACHW4Z_001143"
     CDS             39210..39797
                     /locus_tag="ACHW4Z_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR8880873.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39971..40903
                     /gene="pbpG"
                     /locus_tag="ACHW4Z_001144"
     CDS             39971..40903
                     /gene="pbpG"
                     /locus_tag="ACHW4Z_001144"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR8880874.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40941..42656)
                     /gene="dld"
                     /locus_tag="ACHW4Z_001145"
     CDS             complement(40941..42656)
                     /gene="dld"
                     /locus_tag="ACHW4Z_001145"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8880875.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42852..45149
                     /gene="bglX"
                     /locus_tag="ACHW4Z_001146"
     CDS             42852..45149
                     /gene="bglX"
                     /locus_tag="ACHW4Z_001146"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR8880876.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45401..46318
                     /gene="osmF"
                     /locus_tag="ACHW4Z_001147"
     CDS             45401..46318
                     /gene="osmF"
                     /locus_tag="ACHW4Z_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR8880877.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46325..47482
                     /gene="yehY"
                     /locus_tag="ACHW4Z_001148"
     CDS             46325..47482
                     /gene="yehY"
                     /locus_tag="ACHW4Z_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR8880878.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47475..48401
                     /gene="yehX"
                     /locus_tag="ACHW4Z_001149"
     CDS             47475..48401
                     /gene="yehX"
                     /locus_tag="ACHW4Z_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR8880879.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48406..49137
                     /gene="yehW"
                     /locus_tag="ACHW4Z_001150"
     CDS             48406..49137
                     /gene="yehW"
                     /locus_tag="ACHW4Z_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR8880880.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49118..49225)
                     /locus_tag="ACHW4Z_001151"
     CDS             complement(49118..49225)
                     /locus_tag="ACHW4Z_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR8880881.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49285..49737)
                     /locus_tag="ACHW4Z_001152"
                     /pseudo
     CDS             complement(49285..49737)
                     /locus_tag="ACHW4Z_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        12023 a        12249 c        13359 g        12106 t
ORIGIN      
        1 aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgaac
       61 gacataatct gaatctgaga caacatctgg caaggctggg acggaagtca ctgtcgttct
      121 caaaatcggt ggagctgtat gacaaggtca tcgggcatta tctgaacata aaacactatc
      181 agtaagttgg agtcattacc caaaacagag tggcgataat catcaccatc gcaaaccaga
      241 acacagcgtg ataattccag atctcggcga cgatgcccgc cactgatcct gcgatgatcc
      301 agcccacgcg cgacgtgttg gtatagagcg tggtggctga acctgcctga ccgggcatca
      361 aatcctgaaa atagagcatc ccgatgccgc ccagaatgcc aataaaaata gcatttagca
      421 gctgcaagcc caacagaatg gcaggtgaat gcgccatcag cattcctgcg taaaaacaga
      481 cgccacccac ggcagcaacg cgcattaaga aacgcttacc cagacgtttg gcgaaatatc
      541 cggcaatcaa catcgtcggg atttccagcc cggcggcggt ccccatcatc acaccggcca
      601 gtttctcggg aagatgcagt tcgttgataa taaatagcgg catgttgatg atgtacaggc
      661 tgttcgagcc ccacatcaat gtacaaatga caaacagcag tagcgtatcg cgacggttac
      721 ggcgcggcgc ttcaaccgtg ccggtcgcca gcggaagctc tttttgcatc gacggtaaaa
      781 acagccacac catcacgccg caaacaataa acgctaccgc tgcgctcaga tacattaccg
      841 taaagctgaa acccatcgct aacgcataag ccagcggtgg gccaatgacc catgccagtg
      901 aaacctgagc gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt ttgtcggcat
      961 gttcacgggc aagggcaaac atttgcgggt tagcggtcga gccaaagctg ctaagaaaga
     1021 cgccaacgaa tagcaaaaca aagtagttgc gattccaagc aaaaagggtg caggccagca
     1081 cgcctaacag gcagcaaaag acaatcagcg atttgcgatc gccgcgctta tcagagcgcc
     1141 cagcgagaaa ctgacttacc agaatcccaa tgacagcgct gccggtaaag aagaatccca
     1201 ccatcgccgg acgggcatgt acttcatcgg taaggaaaat actgagtgtc ggggtttgca
     1261 gagcacccgc aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag gtcaggtcaa
     1321 acgatttcgc gctggagact gcgggggagt tatgcatgct ggtcgatcac tggaagagga
     1381 agacgcggag tttacgctgc ttgtcagaaa ataaacagac agcgaaatca aaagcgccac
     1441 gaataatcaa aatgccattt cactgttgct gattggctgc tgacgctgct gaaatgaaat
     1501 tgctgataac ggattttccc atcagcaatt aggaaaaatg acaaaaaatt gtgcagcaca
     1561 tcaaactttt gctcataact ttacggcttt ccttgcgtgc tgaaacagaa gtattatgct
     1621 ttcttgaaac gtttcagcgc gatcttgtct ttaaccctaa gctaggttgg cgcttttttt
     1681 ctcatagagg ctgaatcgtt tcaattcagc aagagaggag aactatgttc cagttatccg
     1741 tacaggacat ccatccgggc gaaaaggccg gagacaaaga agaggcgatt cgccaggtcg
     1801 ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc atgctggcgc
     1861 gcgaacagca aacctcaacg ttcctcggca atggtattgc tattccacac ggcactaccg
     1921 acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg gaaggcgtca
     1981 cctggggtga cggtcaggta gcgtacgtgg caatcggtat tgctgccagc tcggataaac
     2041 atctgggcct gctacgccag ctgacccacg tactgagcga tgattccgtt gctgaacaac
     2101 tgaagtcagc aacaacagca gaagaacttc gcgcattact gatgggcgaa aagcagagtg
     2161 agcagctgaa gctcgacaac gaaatgctga cgctggatat cgtcgccagc gatctgctga
     2221 ctcttcaggc gctgaacgct gcgcgtctga aagaggcgag ggcagttgac gccactttcg
     2281 tcaccaaagc catcaatgaa caaccgctga atctcggaca gggtatctgg ctgagcgata
     2341 gcgccgaagg caatctgcgt agtgcgattg cggtaagccg tgcggcaaat gcttttgatg
     2401 tggacggcga aacggcagcc atgctggtga gtgtggcgat gaatgacgat cagcccatcg
     2461 cggttcttaa gcgtctcgct gatttgttgc tcgacaataa agctgaccgc ttgctgaaag
     2521 cggatgcggc aacgttgctg gcgctgctga ccagcgatga tgcgccgacc gacgacgtgt
     2581 taagcgcgga gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca ggtaccatgc
     2641 tggtcaatac cattaaacaa tttaacagtg atattaccgt gacaaacctt gatggcaccg
     2701 gcaaaccggc aaacggacgt agtctgatga aagttgtggc acttggcgtt aagaaaggtc
     2761 atcgcctacg ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa gccattggcg
     2821 acgctatcgc tgctggtctt ggggagggcg cataatgagc agacgtgttg ctacgatcac
     2881 ccttaatccg gcttatgacc ttgttggttt ctgcccggaa attgaacgcg gcgaagtgaa
     2941 cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg ccaaagtatt
     3001 aaaagacctg ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca atcaggatgg
     3061 ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt ttccaggttg tacaggggcg
     3121 cacccgaatt aacgttaagc tgacggaaaa agacggcgaa gtgaccgact tcaacttctc
     3181 gggtttcgaa gtcacccccg ccgactggga acgctttgtg actgattctc tgagctggct
     3241 cggtcagttc gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca gcccggaagc
     3301 gttcaccgac tggatgactc gcctgcgtag tcagtgtcct tgcattatct ttgatagtag
     3361 ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac ctaaccgccg
     3421 cgagttggaa atctgggcag gccgtaaact gcctgaaatg aaagatgtga ttgaagctgc
     3481 acatgcgcta cgtgaacaag gtattgcgca tgttgttatt tcactgggtg ccgaaggcgc
     3541 gctttgggtt aatgcctccg gcgaatggat cgctaaacca ccgtcagtcg atgtcgtaag
     3601 caccgttggc gcaggggatt ctatggttgg tggcctgatt tatggcttgc tgatgcgtga
     3661 atccagtgaa cacacactgc gtctggcgac agctgttgca gccctggcgg taagtcaaag
     3721 caatgtgggt attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg tcgacttaca
     3781 accttttaac tgacagcagg agaggcataa tgaaaacgct gctgattatt gacgctaatc
     3841 tcggtcaggc acgcgcctat atggcgaaga ccctgctggg cgcggcggcg cgaaaagcaa
     3901 aactggaaat catcgacaat ccgaacgacg cagaaatggc gattgttctc ggtgattcca
     3961 tcccgaatga cagcgcgctg aacggtaaaa atgtctggct gggtgatatt tcccgggcag
     4021 ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct tacactgcgc
     4081 cggtcactgc gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt gcggtgactg
     4141 cttgcccgac tggcgtagca cacaccttta tggcggctga agccattgaa accgaagcga
     4201 aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg ggtaatgcaa
     4261 tcactcccga ggaagtagcc gcagcggatc tggtgattgt ggcggcagat atcgaagtgg
     4321 atctggcgaa atttgctggt aaaccgatgt atcgtacctc taccggtctg gcgctgaaga
     4381 aaacctcgca ggaactggat aaagcggttg ctgaagcaac gccgtatgaa ccggcgggca
     4441 aagctcaaac ggcgaccact gaaggtaaga aagagagtgc aggcgcttat cgtcacttgc
     4501 taacgggcgt ctcttacatg ctgccgatgg tcgttgctgg tggtctgtgt atcgcgcttt
     4561 cttttgcttt tggtatcgaa gcgtttaaag agccgggtac gttggcagcg gcgctgatgc
     4621 agattggtgg tggttcagcc tttgcgctga tggtgccggt actggcaggt tatattgcct
     4681 tctccattgc cgatcgtccg ggcctcacgc cgggtctgat tggcggtatg ctggcggtca
     4741 gcaccggttc tggcttcatt ggcggtatta ttgcgggctt cctggctggt tacattgcga
     4801 agttaatcag tacgcaactg aaactgccgc agagtatgga ggcgctgaaa ccgatcctga
     4861 tcattccgct aatttccagt ctggtggtcg gtctggcgat gatctacctg atcggtaaac
     4921 cagttgctgg cattctcgaa gggctgactc actggctgca gaccatgggg actgcgaatg
     4981 cggttctgct gggggcgatc ctcggtggca tgatgtgtac tgacatgggc ggtccggtaa
     5041 acaaagcagc gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat ggcccgatgg
     5101 cggcgattat ggcggcaggt atggtgccac cgctggcaat gggcctggca acaatggtgg
     5161 cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa agccgctctg gtattgggac
     5221 tgtgcttcat ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg cgtgtgctgc
     5281 cgtgctgtat cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt ggtgcgaaac
     5341 tgatggcacc gcacggtggt ctgtttgttc tgctgatccc tggcgctatt acgccggtat
     5401 tgggttacct ggtagcaatt attgccggta cgctggtggc gggtttggcc tatgccttcc
     5461 tgaaacgtcc ggaagtggac gcagtagcga aagcagcgta ataaaaggtg tgttacaggg
     5521 cagaaatttc ctgccctgta acatttgatg agcgcaaaga gaatgaccag cttgtgaatg
     5581 taattttatg aatttaaatg gttttatctt tcagtagaaa cttcaaacgt tcatcaaaca
     5641 ctaaggttat ttcctcaagg gcagattatt ttctgccctt ttttagtgtt cctgttctgt
     5701 gcatcccgtc acaaattcct cgcttttgta attcaggcat ttaatcgcat tttgtgagcc
     5761 tgctcaaatc tgcccatttt gcctctctgg cggtaattta ctttacgtaa aacatgcata
     5821 ctatgagcac atgtttaaat atgaacacat gtttaaaccg tctttgcagt atctatattc
     5881 ttgtggattg acagggttgg taatttttta ccagaggacg tgctatgcgc gaaaaggatt
     5941 atgtcgtaat tataggttcg gcgaatattg atgtcgccgg atattcacat gaatcattaa
     6001 attatgcgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagta gggcgcaata
     6061 ttgcacaaaa cctggcgttg ctgggtaaca aagcctggct actgagcgcc gtaggcagtg
     6121 atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggtgtttat gtcgataaat
     6181 gcctgattgt gccgggagaa aatacgtcga gttatttatc attactcgat aataccggtg
     6241 aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct gaatatctcg
     6301 cacagcaccg tgaatttatt cagagggcaa aggtcattgt cgcggactgt aatatcagtg
     6361 aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt gtcgatccgg
     6421 tttcagcgtg gaaatgtgtc aaagtgcgcg accgtctaaa tcagaaccac actctgaagc
     6481 caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt gacgatgtgg
     6541 caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg agcatgggcg
     6601 gcgacggcgt ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg ccgatcaaaa
     6661 ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt gcttcgtgtt
     6721 gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt tcgtcaatgg
     6781 cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt atatcgttag
     6841 tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa tttctccgga
     6901 agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcga ccattatttc
     6961 tcacgggatg ccgttcccac aaaatgccca gaccgcaatt gaagtagaag aaactattcg
     7021 taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga aagtgggttt
     7081 aagcaaagaa aaaattgaat tactgggtcg tgaagggcat aacgtgacca aagttagtcg
     7141 tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg tggcttcaac
     7201 gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa ttggtggtgt
     7261 gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac tggcaaatac
     7321 taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat taaccactga
     7381 gtatttagaa accttcggtg tgccgttaat tggctatcag actaaagcgc tgcctgcgtt
     7441 tttctgccgt accagctcgt ttgacgtcag cattcgtctc gacagcgcca gtgaaattgc
     7501 ccgtgcaatg gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg tagcgaaccc
     7561 gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag atcaagccgt
     7621 cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc tgctggcgcg
     7681 cgttgctgag ctgaccagcg gtgacagcct gaaatccaat atccagctgg tgttcaacaa
     7741 cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt aatttctgac
     7801 gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct gacgccgggt
     7861 ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg ggatggtggt
     7921 gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt tgcgcacggt
     7981 tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact tcccaccggg
     8041 aaaatgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt acagtgatgc
     8101 cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg
     8161 tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta ctgcgctcat
     8221 cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg gcagcatttt
     8281 ccagaaagcc ctcaacatca gcaaaatcga atcttttgtt gcggttacca ctattttcct
     8341 cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcgtga atcgcaacga
     8401 gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcggtga tgattggtta
     8461 tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg cgatccccgg
     8521 cggtattttg tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg tcacatttga
     8581 aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc
     8641 gatgaccggg ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt ttgtcgcaat
     8701 tattgcgctg atcaacggca ttatcggcgg aattggcggc tggtttggtt tcgccaatgc
     8761 ctctctggaa agtatttttg gctatgtgct ggcaccgctg gcgtggatca tgggtgtgga
     8821 ctggagtgat gccaaccttg ctggtagcct gattgggcag aaactggcga ttaacgaatt
     8881 cgtcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac
     8941 cgtggcgatt atatcctttg cgctttgtgg ttttgctaac tttggttcta tcggtgttgt
     9001 cgttggcgca ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc agcttggttt
     9061 acgggcgctg gcagcagcaa cgctttccaa cctgatgagt gcgactattg ccgggttctt
     9121 tattggtctg gcgtaatgct cgatttgtag accggataaa acgtattaag cgttgcatcc
     9181 ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc cggagaattt attctccggg
     9241 tccatctcca gcgccagtcc tgacaactgc tttctgtttt taattagata tcctttcttt
     9301 taattaataa accgtcgtga atgaattgcg cgagaacata taaaagatgt cgataagaaa
     9361 cgcctaaata ctctgccgct tgcgtatgct tttcgtgata aagatcacct tcttgtgaga
     9421 gtaatataaa tgctgccagg cgattcacta acggaaatga ttgattctga gttaaagaaa
     9481 caatattacg ataattttta tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt
     9541 cgtttaataa cagcggacgg taatgtttca tagggagcgc aaggcaccaa cactcttcaa
     9601 tagcctgcac cgcacgcggt tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac
     9661 agggagcggc aaagaaatcg atcagcgaca cgcgaccatt ggcaagcgtg gcgtaaagcc
     9721 tggcgcggcc tcgcgtcaga taaaacagcc atgacggctg ttgcccttcc tgcacaatgt
     9781 agtcacgcgc taaaaaatga aacagccgcg tatcggctga aacatccgtt gaaaagcaat
     9841 ccttaaacgc agattcactc atcaattgct gcttaaggtc attattgtgg atttctttca
     9901 cgatgtttat ccgctatgag atttctcata tttagcatac gtacgctgtt gttaagattc
     9961 aatcattggc ccggtcgaat tttctttgct gtagaaaaaa ataacgcaac tggaaacaga
    10021 ggaaataaac aatggaaaag agaaaaatta ttctggattg tgagccgggt catgatgatg
    10081 ctattgcaat gatgatggca gcgaaacatc cggcaataga tttattaggc atcactattg
    10141 tagcgggtaa tcagacgctt gataaaacat taattaatgg cctgaatgtt tgccagaaac
    10201 tggagattaa tgttccggtt tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg
    10261 ttgccgataa tattcacggt gaaaccggac tggatggccc ggtattcgag ccgctgaccc
    10321 gccaggcaga aagtacccat gcggtgaaat atatcatcga taccctgatg gcaagcgatg
    10381 gcgatatcac tctggtgccg gttggtccgc tttcaaatat cgcggtggca atgcgtatgc
    10441 aacccgcaat cctgcccaaa atccgtgaaa ttgtgctaat gggcggcgct tacggtacag
    10501 gcaacttcac cccatctgcc gagttcaata tctttgccga cccggaagcc gcccgcgtgg
    10561 tgttcacctc cggcgttcca ttagtgatga tgggcctcga tcttaccaac cagaccgttt
    10621 gcaccccgga tgtaattgct cggatggaaa gggtaggcgg ccctgccgga gagctgttca
    10681 gcgacatcat gaacttcact ctcaaaacgc agttcgaata ctacggcctg gctggcggcc
    10741 cggtgcatga cgccacctgc attggttatc tgatcaaccc tgatggcatt aaaacccagg
    10801 atatgtacgt cgaagtggac gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg
    10861 aactgggcgt tcttggcaag cccgctaata ccaaagtcgg catcactatt gataccgact
    10921 ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat caaaacccat taagtgttga
    10981 taacaagccg ggacagtatc gcaagatgca ggcccggcta tttctgatgg gcagaaagcc
    11041 gtcgcccaaa atgacatgaa gagataatca ctatggatgt catgagaagt gttctgggaa
    11101 tggtggtact gctggcaatt gcgtttttac tgtcagtaaa caagaagaag atcagcctgc
    11161 gtaccgttgg cgcggcgtta gtgttacagg tcgtgattgg cgggattatg ctttggttac
    11221 cgccagggcg ttgggtcgca gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca
    11281 gcgacgcagg tagcgcattt atcttcggtt ctctggtcgg accgaaaatg gataccttat
    11341 ttgatggcgc aggatttatc tttggtttca gggtattacc ggcaattatc ttcgtcactg
    11401 cactggtgag tattctctac tacatcggtg tgatggggat cttaattcgc attctcggcg
    11461 gtatcttcca gaaagcttta aatatcagca agatcgagtc attcgtcgcg gtcaccacca
    11521 ttttcctcgg gcaaaacgaa attccggcga tcgtcaaacc ctttatcgat cgtctgaatc
    11581 gcaatgaatt atttacagcg atttgtagtg gcatggcctc gattgctggt tcgacaatga
    11641 ttggttacgc cgcactgggc gtacctgtgg aatatttgct ggcggcatcg ttaatggcga
    11701 tccctggcgg gatcttgttt gcccgcctgt taagcccggc tacggaatct tcgcaggttt
    11761 cttttaataa cctctctttc accgaaacac cgccaaaaag cattattgaa gccgatgcga
    11821 caggggcaat gaccgggctg aaaatcgccg ccggtgtagc gacagttgtt atggcatttg
    11881 tcgccatcat tgcgttaatt aacggtatta tcggcggcgt tggcggctgg tttggttttg
    11941 aacatgcctc gctggagtcc attttaggtt acctgctggc cccacttgcg tgggtaatgg
    12001 gtgttgactg gagtgatgca aatcttgccg ggagtttgat tggacagaaa ctggcaataa
    12061 atgaatttgt cgcttatctc aatttctcac cctatctgca aacggctggc actctggatg
    12121 ctaaaaccgt ggcgattatt tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg
    12181 gggtggtggt gggggcgttt tctgcggttg cgccacaccg tgcgccggaa atcgcccagc
    12241 ttggtttacg cgcgctggcg gcggcgacac tttctaatct gatgagtgcc accattgccg
    12301 ggttctttat tggtttagcg tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg
    12361 tcgaatccgg caacattgtt acaccattgg cactaatgac agcgcgttat cggcagacag
    12421 ggtagggttg tttaagctgc cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg
    12481 ggcgctgtca cggaaattgt ttccctccag aaagctatat accagacccg ccataaaacc
    12541 atcgtcagca ccaaaactgt ctaccgtcgt atgcgctggc acagtcagca aaaattgttc
    12601 accatccttt tcgctgcaat aaactgactc atcgggcaaa taaacaaaca gttgctgaac
    12661 accttgctga tgcaatgcat tcactgcggc attacggtca gcatcgctgg tgatcgcctg
    12721 tccccataaa atttccagct ccggtaaagt gggtttcagg gtgtgaatat gcgccagcca
    12781 gtgtttgatt ttgcccgctt tgaattctga aacggtatcg acaaacaccg ggatctcatc
    12841 agcgagggta aagacccatt ccagcgcctc ggctgtcagg ttgcaatcag ccagtaccac
    12901 gcccgcatga cgaagtaaat cgcgcgaccc gttcagtagt tgcggtgtca actgttccag
    12961 cagatgggta tcgttaattg ccagcacggt ttgatcgtct cgattggcaa ttgccagata
    13021 cgtcgatgtg ctttgaccat gcaaacgaac gcagccggag acattcacgc cggcacggcg
    13081 cgtttcttcc aggagcattt cgccataaaa gtcatcgcca atcactgaaa gcaaatggac
    13141 gtcacggcct aacagcgcca gattgtgggc gatattgcgt ccaacgccgc ctgccgaaca
    13201 atgaattgta ccgggatgag aagccgcttg cgggtaatgg atatccgcca tcccgcgaat
    13261 atccatattg attgtcccca ccactacgca gtattcctgc tcggtgagaa tgtaaccttt
    13321 gtctttaatc cggcctttgc gcattaaatc cataatatgc gccgcaacgc gcgaacgact
    13381 gatttgcagc atgtccgcaa tttcgttctg ctgaatcagc gggttacgcc gtaaaattgc
    13441 aaggatctcc ttttcccggt tatttatctt caggctaccg ctttttcagt ttgttgcgct
    13501 ttcagccagg cgatctcttc tgcccagata tccgggttga tggtttcaag gatcagcgga
    13561 atgccgtcga aacggtcgtt ctgcataatc cagcggaacg catcatgacc gatattgcct
    13621 tcaccgaggc tatgatggcg gtcaacgcgg ctgccaaagg tacttttcgc atcgttaagg
    13681 tgcatcccgc gcaaatactt aaagccgaca atacgggcaa aatcagcgaa cgttttttcg
    13741 cattcggctg gagtacgcaa atcatacccg gcagcgaaag catggcaggt atcaatgcag
    13801 acgccgacgc gggatttatc ttccacgccg tcgataatcg ccgcgagatg ttcgaattta
    13861 aaccctaagt tactgccctg accggcggtg ttttcaatca ccgctgttac accttgagtt
    13921 ttatccagcg caatgttgat ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc
    13981 tgcatcagat ggctgccagg gtggaagttg agcaaagaaa gccccagttg ttcgcaacgc
    14041 tgcatttcat ctataaaggc atcgcgcgat ttttccagag cttcagtgac cggatgtccg
    14101 aggttgatca gataactgtc gtggggaaga atttgcgccg atgtgtagtg atatttttca
    14161 caggcggctt tgaattcatc gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt
    14221 tggtttttgg tgaacaaggc aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca
    14281 tttgccagac cgccagcagc actaacgtgc gctccaatgt atttcatgcg aggactcctg
    14341 ttaaacccgc tggaggaaaa cggtaatgat agcgggttaa caggagcaag aagtagtggt
    14401 ttatgcgatg acgctttgaa tcacatagtt aatcgcacca ccaccaacaa tcagccaggc
    14461 aaacagtacc agtgccatca gcagaggttt cgccccagct tttttcagcg cgctgacgtg
    14521 agtggtcaga cccagcgccg ccatcgccat tgccagcagg aaggtatcca gcgttaccag
    14581 catgttcacc acgctctgcg gtaacaggtg gaacgagtta aagatggcaa ctacgatgaa
    14641 caagatggca aaccacggaa tagtgatttt gcttttctcg ccgctgttcg ccccagacag
    14701 ctgtttaaca cgcgccgcca gcaggatgag gaacggagcc agcatcatca cgcgcagcat
    14761 tttggaaata actgctgcgt tttctgcatc cgggctgatg gcatgacctg ccgccaccac
    14821 ctgcgccact tcgtgcacag tagaaccaat gtagataccg aaagtttccg gactaaacca
    14881 ttgagacatc agcggatata tcgccgggta gaggaaaatc gcgacggtcc cgaagataac
    14941 aacggttgca acagccacgg ttactttact ggcttccgct ttcactaccg gctcagtcgc
    15001 cagtaccgcg gcagcaccac agatactgct accggcaccg atcaaccaac tggtgtgctt
    15061 atccagacca aacactttct gccccaggaa gcaagccagc aggaaggtac tggacagcgt
    15121 cagcacgtca atgatgatcc cactgatacc gacatcggca atttgcgaga acgtcagacg
    15181 gaagccataa agaatgatac ccagacgtaa taaatattgc ttggcaaaca gcacaccacc
    15241 gtcacagctt ttccagatgt gcggatagat ggtgttgcct aaaaccatcc ccaacaagat
    15301 tgcgagggtg agggcactaa acccggcacc cgcaaccgcg ggaatggaac caccccacag
    15361 ggcgaccccg gtgataactg cactcagggc taaccccgga ataaaatgcc acagtgtacg
    15421 atgttgtttc tgtaaggtga tattcgtcat aaccctctcc tttaaccggg cataaggtta
    15481 cgactaactg gtttaaaaat aaaattgatt atatatttat aattaatctt tataagtggt
    15541 aagcgactat gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg
    15601 gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct
    15661 tgactgacct ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg
    15721 ttgttaatga acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg
    15781 ttgaaatcga acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta
    15841 ccatcggtaa ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt
    15901 tgccgattga acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc
    15961 gcgttgatat tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt
    16021 ggctggaaga cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg
    16081 tcaccttaga acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc
    16141 gggatattgt cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat
    16201 taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt
    16261 cgcgacgtgt gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc
    16321 tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg
    16381 cgctacggcg ctttctggac tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac
    16441 aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc cttcagcatg cattcgccag
    16501 aaaagagatt ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg
    16561 tgtatttctg ccggaaggat tgccaatcgt ctgctacaat cgcgccatca tttttaagat
    16621 ggatagcatt tttgtatggg ttccgaaact aaaactacag aagcgccggg cttacgccgt
    16681 gaattaaagg cgcgtcacct gacgatgatt gccattggcg gttccatcgg tacaggtctt
    16741 tttgttgcct ctggcgcaac gatttctcag gcaggtccgg gcggggcatt gctctcgtat
    16801 atgctgattg gcctgatggt ttacttcctg atgaccagtc tcggtgaatt ggctgcatat
    16861 atgccggttt ccggttcgtt tgccacttac ggtcagaact atgttgaaga aggctttggc
    16921 ttcgcgctgg gctggaacta ctggtacaac tgggcggtga ctattgccgt tgacctggtt
    16981 gcagcacagc tggtcatgag ctggtggttc ccggatacac cgggctggat ctggagtgcg
    17041 ttgttcctcg gcgttatctt cctgttgaac tacatctctg ttcgcggctt tggtgaagcg
    17101 gaatactggt tctcactgat caaagtcacg acagttattg tctttatcat cgttggtgtg
    17161 ctgatgatta tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc
    17221 ggcgaagcgc cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat gattgtcggc
    17281 ttctctttcc agggaaccga gctgatcggt attgctgcag gcgagtccga agatccggcg
    17341 aaaaacattc cacgcgcggt acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc
    17401 gcgatcctga ttatcagcct gattattccg tataccgatc cgagcctgct gcgtaacgat
    17461 gttaaagaca tcagcgtcag tccgttcacc ctggtgttcc agcacgcggg tctgctctct
    17521 gcggcggcgg tgatgaacgc agttattctg acggcggtgc tgtcagcagg taactccggt
    17581 atgtatgcgt ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt
    17641 ttcgctaaat tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt
    17701 gccggtttgt gcttcctgac ctccatgttt ggcaaccaga cggtatacct gtggctgctg
    17761 aacacctccg ggatgacggg ttttatcgcc tggctgggga ttgccattag ccactatcgc
    17821 ttccgtcgcg gttacgtatt gcagggacac gacattaacg atctgccgta ccgttcaggt
    17881 ttcttcccac tggggccgat cttcgcattc attctgtgtc tgattatcac tttgggccag
    17941 aactacgaag cgttcctgaa agataccatt gactggggcg gcgtagcggc aacgtatatt
    18001 ggtatcccgc tgttcctgat tatttggttc ggctacaagc tgattaaagg aactcacttc
    18061 gtacgctaca gcgaaatgaa gttcccgcag aacgataaga aataagtttc ctcccttcct
    18121 tgctaagccc tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg
    18181 atataaaatt taacatttgg attgataatt gttatcgttt gcattatcgt tacgacgcaa
    18241 tcaaaaaagg ctgacaaatc agaggctgtt ccggctttct gggatgatca cctgcataca
    18301 aataagtcca ccgcgatgct gccgtacgca aggggacgta aagaagatgt gagcgatacc
    18361 cattttattt tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta
    18421 cgggtcgggc tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat
    18481 gatggcgaaa cgatggttgt cactgcatct tccgtggaac aaaatcttaa agatgcaccc
    18541 gccagtatca gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag
    18601 gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc
    18661 gttagtattc gtggtctgga cagcagctac accctgattc ttgtcgacgg taaacgcgtt
    18721 aactcccgca atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat
    18781 tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg
    18841 ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt
    18901 accgtcgata ccaccattca ggaacatcgc gatcgcggtg atacctataa cggtcagttc
    18961 tttaccagtg gaccattaat tgatggtgtg ctgggaatga aagcttacgg cagcctggca
    19021 aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt
    19081 attgaaggat tctccagccg cgacggcaat gtcgaatttg cctagacacc gaatcaaaat
    19141 cacgatttta ctgccggata cggtttcgac cgtcaggatc gtgattccga ttcgctggac
    19201 aaaaaccgcc tggaacgcca gaactactgc gtcagccata atgggcgttg ggattacggc
    19261 accagcgaac tgaaatccta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc
    19321 ccgataactt ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt
    19381 aatcagtttc tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac
    19441 ctgaccgggg gaaccagctc caaaacgtct gccagccagt acgcgctgtt tgtggaagat
    19501 gaatggcgga tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa
    19561 acctacggtg aacactggag tccgcgtgcc tacatggttt ataacgccac cgacaccgta
    19621 acggtgaaag ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct
    19681 gactggacga gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa
    19741 ccagaaacca gcgaaagttg ggagctgggg ctttactaca tgggtgaaga aggctggctg
    19801 gaagggggtg aatccagcgt tatcgttttc cgtaacgatg tgaaagatcg tatcagcatc
    19861 agccgtacgt ctgacgtcaa cgctgcaccg ggctaccaaa actttgttgg ttttgagacg
    19921 ggcgctaacg gacggcgcat accggtattt agctactaca gcgttaacaa agctcgtatt
    19981 cagggcgtgg aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcgatcaac
    20041 tacacctaca acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg
    20101 ccgttccata ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca
    20161 ttctatgttt ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca
    20221 ccgggcggtt ataccatctg gaataccggc gcggcctggc aggtgactaa agacgtcaaa
    20281 ctgcgcgcag gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat
    20341 aacgaagacg gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc
    20401 cggcgaaccg ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct
    20461 ctataaaaga ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca
    20521 tcggccacgc tttctggcgc gcggcttctg ccaataccgc aggttgcaac tggtcggcaa
    20581 gaaactgatc attatcgccc tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg
    20641 catacatcag cgcgcaacta tcccattcca gcatgcattt ttgtcctcac ctaaataggt
    20701 gctaaacgct ttgattcccc acgggacgct gcacggattc acaatgggcg caaaggccga
    20761 aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat
    20821 tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg
    20881 taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc
    20941 attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc
    21001 gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttccgccg ctacccgctg
    21061 ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag
    21121 cactggtggc ggggtgtgat cacgaggggg ggggagaaag atactgaacg tcatcgggca
    21181 gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc
    21241 ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt
    21301 ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc
    21361 tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg
    21421 ccgcctcacg cacaataatc aagctgtaaa tcagcttaat aactttgccc ccacgcaggg
    21481 tggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc
    21541 tggttcatga agcgttagtt gcgagaggac tggaaacacc gctgcgcccg cccgtgcatg
    21601 aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc
    21661 tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa
    21721 tgtatgtcga tgaaattttc tccggtctgg attacgccaa tttcccgaaa atcaccctca
    21781 ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta
    21841 cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag
    21901 attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc
    21961 aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca
    22021 ataacgtggc tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg
    22081 atgcaaccag tgccacgaca acgacctctc ttggtggatt gttcaaatcc agtcagaata
    22141 cgcgccacga gtttctgcgc gctgtgcgcc atcacaactg attaaaaggc aggaaccatg
    22201 gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta
    22261 aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg gtacgatgct
    22321 attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc
    22381 ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga
    22441 cgctggatcc agtcgcggtt aacgctgtta gtcttgttgg gctttattca cggtttattg
    22501 ttctgggacg gcgatattct gctggcttac gggctggtgg ggcttggcgt tttgctgtta
    22561 ttggggctga tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt
    22621 ttgtatgaaa aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat
    22681 ggtgttggca acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg
    22741 atgctcattg gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt
    22801 cactatcgtc gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc
    22861 atcgccctgc aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg
    22921 ccgcgggaac tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc
    22981 tggccgcaat tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg
    23041 ctgaccaact atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg
    23101 tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat
    23161 atcctcttct ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg
    23221 cgtcagttaa ctttgcgtgc tgccggaccg gcaatatcta aaacatcaag ataacgatct
    23281 ggattacatt cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga
    23341 agtcctgtat tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca
    23401 taacaggtag tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg
    23461 gcaacggttt cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca
    23521 gtaatgaaag ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca
    23581 actcaggtta gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc
    23641 gcgctggtaa aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc
    23701 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt
    23761 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg
    23821 gataacattc ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
    23881 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
    23941 catcaacgta ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa
    24001 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
    24061 actggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
    24121 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
    24181 ttaaaagata atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt
    24241 cccatcgccc gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg
    24301 gctaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc
    24361 agccactgtt ttatgccgac gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg
    24421 gcggcgatca ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt
    24481 cacagtatct taacaatgtg atagctatga ttgcaccgtt ttaatgttgt aacccgtatg
    24541 taacagtgaa taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt
    24601 tctggagcgt taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta
    24661 acacttcatt aactctactg cctcgccgag catttatctc aagcactacc ctgcataaga
    24721 aaaaccggag ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg
    24781 ttattcggtg ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac
    24841 gacgataact ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca
    24901 gatgttcagc tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc
    24961 gacgtattgc tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct
    25021 gcgggtacgg tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa
    25081 gaaccgtctc gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc
    25141 aaagagtccg gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt
    25201 tgggatctga acaaagacgg tcagattcag ttcgtactgc tgaaaggtga accgggccat
    25261 ccggatgcag aagcacgtac cacttacgtg attaaagaat tgaacgataa aggcatcaaa
    25321 actgaacagt tacagttaga taccgcaatg tgggacaccg ctcaggcgaa agataagatg
    25381 gacgcctggc tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat
    25441 gcgatggcaa tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg
    25501 tttggcgtcg atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc
    25561 acagtactga acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg
    25621 gccgatggca aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc
    25681 gtaccttatg ttggcgtaga taaagacaac ttggctgaat tcagcaagaa ataagactga
    25741 ttgggcgcag tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc
    25801 agctcaacga ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtcc
    25861 tttcctggtg ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat
    25921 gcattaatgg gggaaaacgg tgcaggaaaa tcgacattat taaaatgcct gtttggtatt
    25981 tatcaaaaag actccggcac cattttattc cagggtaaag agatcgattt ccattccgcc
    26041 aaagaggcac tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa
    26101 cgttcggtga tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat
    26161 caggacaaaa tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat
    26221 ccgcgtgcgc gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa
    26281 gcgttttcct ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa
    26341 aaagaggtca atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt
    26401 gtttatatct cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg
    26461 cgcgacggtc agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc
    26521 gccatgatgg ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc
    26581 gaagtcatcc tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc
    26641 tcgtttgatc tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt
    26701 accgatattg ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg
    26761 cacggcaaac agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg
    26821 gtaactgagg agcgccgctc aacgggaatt tatgcctatc tggatattgg ttttaactcg
    26881 ttaatttcca atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg
    26941 aaaagcgata cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg
    27001 caaattggtt cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta
    27061 acgcaaccag aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag
    27121 tttgaaattt atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc
    27181 tcctctgaaa tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt
    27241 ctcgtttccg gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg
    27301 tctttgcacc tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt
    27361 acttacctga aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt
    27421 ttccaggacc caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg
    27481 gtgcgtatta ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt
    27541 tctgctggtc gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg
    27601 gataacgcca acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg
    27661 attgtctgtg ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc
    27721 aacgtgacgc cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg
    27781 ctctattacg actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc
    27841 tttgctcagg gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg
    27901 ttgattgcgg tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt
    27961 tttgccattg gcggtaaccc ggaagcggca aaaatatctg gtgtcaacgt cggcctgaac
    28021 ctgctgatga tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc
    28081 ggacgtatcg gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg
    28141 gcgtgcgtgg taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg
    28201 accggggtaa ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca
    28261 tactggcagt acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg
    28321 aaatacgcgc gtaagaagtg atgtgattgc ggatattgcc ggatgcgtct ctggttttac
    28381 aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa
    28441 cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc
    28501 ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata
    28561 taacagcgtc cacagccaac acatttgtca agattgatac ggggatagac aatataactg
    28621 cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac
    28681 gaatcaaatc cctgatcggc gagataatgc gataacccgc tcgccatatc ttctacgatg
    28741 cgatagccgt actgcataat gccggtggtg acctgtaacg ttgctgcgcc gagcaataaa
    28801 aactccgccg catcttccca ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc
    28861 agttctggat gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt taccgcttta
    28921 ccggaatatc cggaaatact cgatttaccg ttaacgatcg gcataccgat tttctgatta
    28981 agatcgatat tggtgatgga tttaacggtg ttaattgccg caatgccatc tgcgccgccg
    29041 cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat cttcgccagc
    29101 attggcagcg tggaaccccg tttcactgcc cgacaatatt tttctaccag ctccgggctt
    29161 tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa gttacactcg
    29221 atcatatccg cgccagctgc tcccattgct gctcattttc ccccatgatc gaagcgatca
    29281 atactttgtc cgggtaatct tccttcagcc gacgcagggc ggtcagattc tcttccaacg
    29341 gatgttcagc aatctgctcc atatttttga agccgataaa accggtatct tctttcacca
    29401 gatgatcaaa acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt ttaaacacca
    29461 caccgcccca acctgtgtcg taggctttgg cacacatctc atagcagttg cctaccggcg
    29521 aagaagagag gcagaacggg ttgggaaagt tcacgccgca aaaagtaatc gaaagatctt
    29581 tcgttaacat gagcaagctc cctctaaata gtgatgaatc gcctcggcgg cttctttccc
    29641 ggttttcacg gcatagacca cggttttgtc accctcaaca atatcgccag cagcaaagac
    29701 ttgcggatcg cggatctgat aattttgtgt tttgatggtg ttacgctgct gctctaactc
    29761 cgcaaaggca tccagtctgg catgctgacc gacggcgaga ataattttat ctgccgccat
    29821 cgtcagttcg cctgataacc gtacatgctt aaacgtgact ttattgcctt ccacggctac
    29881 tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa actctttttc
    29941 gcttgccgga aactcatcta actcttcacg tgctacgcaa gttaccgcct gacagccgag
    30001 aactttcagc gtgctggcta cgtccatcgc gacatcaccg ccgccgataa ttaatgcgct
    30061 ttgtggaatg ctgatatcgc cttgcgcctg tcgtacacgt tgcaggaagt cgacggcaat
    30121 ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg cttgataacc ccacggtgac
    30181 cagtaccgcg cggttttccg ctttcagttg ctccagggtg agtgttttac cgatttcgtt
    30241 attgcactta atggtcacgc ccattttttc aatacgggcg atctctgcat ccagcactga
    30301 ttgcggtaac cggaattgcg gaataccgtt acgcaaccag ccaccggggt gcgcttcttt
    30361 ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtc acactggcct gcaatccggc
    30421 aggacctgcg ccaataatcg cgactttgcc gagcgtttta gtaccgggct gataaatttc
    30481 catcccggtt tgttgttcaa aatcagtaac aaaacgctgt aagcggccga tatcaatggg
    30541 agcatcaaca ccggcacggg tacaaccgct ttggcataat ttctccgtcg ggcacactct
    30601 ggcacaaacg gcaccgaggg cattattttc gcgaattgtc tcggcagcgc ctttaaaatt
    30661 acgaaagtag attgagcgaa taaatttccc cggatcggtc tgtgccgggc aagcctgact
    30721 acagggagcg tcgtgacata ataaacagcg agaggcttct ttaatcgcca gtaacggcgt
    30781 aaaagccgga gtgagttcat ccagataatt ttgttgcggc atgtgatctt ccttaatgct
    30841 atgagtaatt atggcgaacg ctaaataaat attgcgtgct gatattaagg ctatgcgcgt
    30901 ggggaaattt aagctgtgaa aggattcaca aatagctgcg gttcagaatg ttatatccgc
    30961 agcttattga gtgaagtaat aattattctc aataattaac agaaaagaga aaatattaac
    31021 gatagaaaat aagataaaca atcatgccga tagataaaat tccgaagcca atatatgcca
    31081 ccatataagc gaagaagagt ccgggatttt ttgctcgcca gacatacacc ggttccggtg
    31141 catttttaac ccgtttatca atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac
    31201 aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta
    31261 ctttccttgt ttcttttgta attcaagtaa ctgttcgggt gtaacggcgg gatacccctg
    31321 cgcatcttcc ggcacctggt gcatagccgg aatagggacc agcggcccta aaaaacgcgg
    31381 ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa cgggcggcaa attcgcgaat
    31441 acgtactgac agcatatctt tcggtgacgg tacggcataa cactgagcct gaatccccat
    31501 atgcagcgca ataaataatg ctcgctcaca gtggaaacgt tgggtgataa taatgaaatc
    31561 attagtatcg aaaactttgc gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta
    31621 atcgagaaca atatctgatg ggtcgacacc agcagcgatt aaatctttgc gcatggtcat
    31681 cggctcgtta taactttgca atgcgttatc gccgctcagt aatagataat ttaccttacc
    31741 gctgttatag gcattaatcg ctccttgaat gcggtagcga taatactgat taataacgcc
    31801 ggtacgataa tattttgctg tgccgagcac cacgccgacc tggcggtagg ggagatcctg
    31861 caattcgtcg tagatataag gcgcggtttt ccagctcatc cagcgatcga ggccgagcac
    31921 agtcaacagc agcaagccga tcaggactaa caggctgagg aacacgcgct ttaacatgca
    31981 ggagactcga gctaaggaaa atttccatca ggctacttta cccgctggtt ttgcgcaaga
    32041 aacggcaaac tgtttgccgg gaatttcgac accaggagag ggctggcaag ccggacaagg
    32101 cgttcacgcc gcatccggca ccaggcttaa gcgagaagca cgcggtcgat actgtgacag
    32161 ccgagagctt tcagcgttgc ggaggtggca tcccactgaa tcaacggcgc atcggctttt
    32221 tctgccagaa ccgcgcgctg gatatccggg taatcataac ccttgagatt caacagaatt
    32281 aacgccccct gcaacggcgg cagagtcggg ttgaatgcgg cgttttcagc gtagctgccg
    32341 ctgaaaatac gaccgtcttt acattccagc gcgacaccgc ttggtgactt actgtaaggc
    32401 atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca
    32461 tagccgtgat cctgctcgtc catcagcagc gttttaatct ccagatcttt cggcccaaag
    32521 gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc ccggcaaatg aatacgcaga
    32581 tccagaccgc tgttcagttc attcataaac tgacggcagt gaccacaagg cgtatagtta
    32641 acggtgatgg ctgcaagcgc tttttcacca ctcaaccaag cgtggctgat cgcgctttgt
    32701 tcggcatgaa cggtttgctg cattgttgca ccaataaatt ccatattggc accaaaatac
    32761 caggttccgc tcacaccgcg cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc
    32821 gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg cgtcttcgtc cagtcccgtt
    32881 gcgctcttca gcgatgagac ttgctccccg gtcaacaaag cggggaagta cttgtctgcc
    32941 agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt gggcaaaagc ggtttgaaaa
    33001 cgtggatgca tggcgttgcc tcataatcat gtaatgggat aacagtttac gaacccgtta
    33061 tggctttata tgtgatctga atctcattaa ttatgcaaca cgtgaaactt acatcaaaaa
    33121 tgcgcgacgt atcgcaaatt ttagcccatt actgccagaa taatcgggaa aaggaacggt
    33181 gcgatcagcg aagtaattat cccgcataac accagcgcta gcgaactaaa tgcaccttcc
    33241 tgataatcca gctcggcgca gcgcgccgta ccgagggcgt gcgaggcagt ccccattgcc
    33301 agaccgcgcg cagctttggt acgaatacgc atcgcattaa gcaatgtatg gccaaatacc
    33361 gcgccgagga tgccgacgaa aatcacgcaa actgcgctga ttgccggaat accgccaata
    33421 ctgccgccaa ccgccattgc aataggcgtg gtgactgatt tcggcaggat tgacgcggcg
    33481 atttccggtg aagcgcccat caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg
    33541 ctgccgataa aacagatggt gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc
    33601 tcatataaag gataggccag cgcgacgacc gccggttgca gcagatcgtt tagcacttcg
    33661 ctacctttaa agtaactgtc gtaagagatg ccggtcagca ttaaaaaagg aatgatcacc
    33721 accattgcta ccagcaacgg gttaagcagg ggaaacttat accgtgccgc cagtttgcgg
    33781 gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc agatattcgc catcattctt
    33841 ctgatccttt ctgacctacg actttacgtt caccgtgtac cagttgtgaa ctccagctca
    33901 ccaccagaaa aaccaccagc gtactgacag cacaggaaac cactaccggg ccaaactgcg
    33961 cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat cggcacaaac aatagcgcca
    34021 tatagcgaat cagtacgtag caccccggat tgacccattt cgccggaaga atttgcaagg
    34081 ccagcaggac aaacaggatc agcatcccga tgatgctgcc cggaatggtt accggtagca
    34141 gggaagcaat gaaaatgcct gcatacaggc aggcataaat caggacgaaa gcgcgtaaat
    34201 attgccagat aatgttcagt gtcttactca taatcaacac cccagatgag atggattcat
    34261 catacaatta attcgtaaaa cgtgctaccg atcacattaa gaaatcagcg cttatttttt
    34321 cgctacccgg tattacgcaa tgagcagcga actcaggcat acatcaagca tagagaggga
    34381 ttgcagtttg cagcaatccc tctttgcaga cctttaatcc gctgaccata ggcgctaaca
    34441 tagcgcctca tttttttgcg ggtgatgata tgcgtgtgtt actggcaccg atggagggag
    34501 tgcttgactc tctggtgcgt gaattgctga ccgaagttaa cgactacgat ctgtgcatca
    34561 ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa agtctttcat cgcatttgcc
    34621 ctgagctaca aaacgccagc cggacaccat ctggtacgct ggtgcgcgtg cagttgttag
    34681 gtcagttccc acaatggctg gcagagaacg ccgcccgtgc ggtggagtta ggtttctggg
    34741 gcgtggatct caattgcggc tgcccgtcga aaacggttaa cggtagcggc ggcggggcga
    34801 cgttactcaa agatcctgaa ctcatctacc agggggcaaa agcgatgcgt gaagctgtac
    34861 cggcgcattt gcctgtcagc gtgaaagtgc gtctgggctg ggacagcggt gagaagaaat
    34921 ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga gctggtggtg catgggcgga
    34981 cgaaagagca gggttaccgc gcggagcata ttgactggca ggcgattggc gagattcgcc
    35041 agcggctgaa tattccggtg attgccaacg gtgaaatctg ggactggcag agcgcgcaac
    35101 aatgcatggc gatcagcggc tgcgacgcag tgatgattgg tcgcggggcg ctcaatattc
    35161 ccaacctgag ccgggtggta aaatataacg aaccgcgaat gccgtggccg gaggtggttg
    35221 ctttgctgca aaaatatacc cgtctggaaa agcagggcga taccgggtta tatcacgttg
    35281 cgcggattaa acagtggttg agttatttgc gtaaagaata cgatgaagca acggaattat
    35341 ttcagcatgt tcgggtgttg aataactccc ctgatattgc aagggctatt caggcaattg
    35401 atatcgagaa actctaaaaa ccatagtgat gtttttgaca atgtgttagc tctttatcaa
    35461 taggccgcac ggttgtttgg tggcgtcaaa atcgccgagg cgttccctga aggccggggc
    35521 agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgaccc
    35581 ggtagccgta agggataagc cgagggcacc gcgcagcggc gattttgttc gccagagccc
    35641 gggggggcag ggggcggcgg cgattggtcg ccccctgcgc gctccttgcg ccagtgatta
    35701 tatgaagcaa tgcacatgac aggagcgcaa caggatgaga gactgaaata ttctcgttat
    35761 tttcggatct gatgacatta attaatttta aaatatcatc ttaaatacgc cagtcaccac
    35821 cagtagccca atcaggaaaa taatcaatac agcccagagt ataattttca ttggcgttcc
    35881 tttatttgtg agtgctgaat aagtatagga aaaaacgaag ccctttgccg ttatccataa
    35941 acgattaaga taaatcggaa gatatcattt acttagtcta tatttttctg ggtatgtcgt
    36001 tttattgcta ctgatgtatt gccacgcgaa actaaaaaat atatcgcaac acacggtaat
    36061 ctccgcgcac cgtttcttac ctgattattc ccccatgaat cgtgattcct tttatccagc
    36121 catcgcctgt tttccgctgt tactgatgct ggccgggtgt gcgcctatgc atgaaacccg
    36181 ccaggcgtta agccagcaaa cgcccgctgc acaagttgac accgcattac ccacggcgct
    36241 gaaaaatggt tggccagaca gccaatggtg gctggagtat cacgataatc agctcacttc
    36301 attaattaac aatgcactac agaacgcacc ggatatgcag gtggctgaac agcgcattta
    36361 gctagctgaa gcccaggcga aggcagttgc cactcaggat ggtccgcaga tcgacttttc
    36421 ggcggatatg gagcggcaaa aaatgtcggc agaagggcta atggggccgt ttgctctgaa
    36481 cgatccggcc gcaggtacga ccggtccgtg gtacaccaac ggaacttttg gcttaacggc
    36541 gggctggcat ctcgatatct ggggaaagaa tcgggcggag gttactgccc gcctgggtac
    36601 ggttaaagca cgggcggcgg aacgcgagca aacccgccaa ttgctggctg gcagcgtagc
    36661 ccgcctgtac tgggagtggc aaacccaggc ggcgttaaac acggtcttgc agcaaataga
    36721 aaaagagcag aacaccatta tcgcgaccga tcgccagcta tatcagaacg ggattacttc
    36781 ttcagttgac ggtgtggaaa ccgatattaa tgccagcaaa acccggcagc agctcaacga
    36841 tgtcgcgggg aaaatgaaaa ttattgaggc acggttaaat gcactcacaa atcaccagac
    36901 taagtcattg aagcttaaac cggtcgcgtt gccgaaagtg gcaagccagc ttcctgatga
    36961 actggggtac tccttactgg cccggcgggc agatttgcag gcggcgcact ggtacgttga
    37021 gtcatcgtta agcactattg atgcggcaaa agcggcattt tatcctgaca tcaacctgat
    37081 ggccttcctg caacaggatg cgttgcactt aagcgatctg ttccgtcatt ccgcgcagca
    37141 aatgggcgtt acggcaggcc tgacgctacc tattttcgat agtggtcgtc ttaacgccaa
    37201 tctcgatatc gcaaaagccg aaagcaactt gtctatcgcc agctacaaca aagcggtggt
    37261 tgaagcagtg aatgacgtgg cgcgggcagc cagtcaggtt cagacactgg cggagaaaaa
    37321 ccagcatcag gcgcaaattg agcgcgatgc cttgcgtgtg gtaggtcttg cgcaggcccg
    37381 ctttaacgcg ggcatcattg ctggttcccg cgtcagcgaa gccagaatcc cggcgctgcg
    37441 tgagcgggcc aatggcctgt tattgcaagg gcagtggctg gatgcctcca ttcgactcac
    37501 cggtgcgctg ggcgggggtt acaaacgctg atgatatgcg ccttctatac ttaacgttta
    37561 ttcagcgtta agtggagaac tcgatggcat aggtcgcgat aattaccgct tccgattcgg
    37621 ggatcggcaa agagtgcgcg ttattactgg cgcagcaggg gtttgatatt ggcattacct
    37681 ggcactcaga tgaagaaggg gcaaaagata ccgcgcgtga ggtggttacc cacggcgtac
    37741 gtgcggagat cgtgcaactg gatctcggca atctgccaga aggtgcgcag gcgctggaga
    37801 cactcatcca gcgatttggg cgtattgatg tgctggtgaa taatgcgggt gcaatgacca
    37861 aagcgccgtt tcttgatatg gcttttgatg agtggcgcaa gatttttacc gttgatgttg
    37921 atggtgcatt tttatgctcg caaattgcgg ctcgtcagat ggtgaaataa gggcagggcg
    37981 gtcgcatcat caacattacg tcggtacatg aacatacgcc gctgccggat gccagcgcct
    38041 acacagccgc taaacatgcg ctcggcgggt taaccaaaac gatggcgctg gagttggtca
    38101 ggcataagat tttggtgaac gcagtcgcgc ctggggcgat agccacgcca atgaacagca
    38161 tggatgatag cgacgtgaag cccgacgcgg agccttcgat tcctctacgg cgttttggcg
    38221 caacgcatga gattgccagc ctggtggtgt ggctttgttc ggagggcgca aattacacga
    38281 cagggcagtc gttgatagtg gatggcggct ttatgttggc gaatccgcag ttcaacccag
    38341 aatagcgcag tgaggtgcgt ggatatttca atgggttagg ccagataaga caccagcgtc
    38401 gcatcaggca tggaacaccc ggtgccggat gtggagtgaa tgcttatctg tggcaactgc
    38461 tttacgcctg attatccggc tttttcttcc cgcgccgttt cagccaccag cgcacgccca
    38521 ccaccagaac cacgaccaga atcaaccaga cccagtgctt caaatgctgg tcgagattgt
    38581 gcaaccacgg cgcaatcacc tgaccaccag cgtaaccaat agtggtaaaa atcaacgccc
    38641 aggcaaatgc gccgagaata ttcagcggca gaaagatttt cggcggcagc tggctggcac
    38701 caatcagcgt cgggccaatc acccgaaagc catacataaa gcgcgtacca atgacaaaca
    38761 gatacggatg gcgttggata agtttctgtg cccgctcaat tttatcctga tgtttcgaga
    38821 aacggcgtaa cagcttgccg ccaaaccgcc gcccgcacag atagagcacc tggtcgccaa
    38881 tcatgccacc aagcgccaca gaaagtacca ccagtgggaa ctttaatagc ccctgatgtg
    38941 ccgcaacgcc tcccagcaaa gtcacggttt caccttccgc caggctaccg ataaccagcg
    39001 cggcataacc atattgtgag atgagtgtat tgagatccat aggccagttt gtctcccttt
    39061 ggttgcctgt aagcatacac cctgtgaatc attcacgctg aaaaaggaca tacatcagac
    39121 ggtgcaaatt acatggatga acaaaaaata atcacaagct ggcttatact tgaactgtca
    39181 ggttcatgaa ctcacaacaa gggggcggta tgagccatgt ctggggactg ttttcccatc
    39241 ccgatcgtga aatgcaggtg attaatcgcg aaaacgaaac gatttctcac cattacaccc
    39301 accacgtttt gctgatggcg gcgattccgg tgatttgcgc cttcattggc actacacaga
    39361 ttggctggaa ttttggcgat ggcactatcc tgaagttatc ctggtttacc ggactggcgc
    39421 tggctgtctt gttttacggc gttatgctgg ctggggttgc ggtgatgggg cgggtcatct
    39481 ggtggatggc gcgcaattac ccgcagcgtc cgtctcttgc gcactgcatg gtcttcgcgg
    39541 gctacgttgc aactccgctg tttttaagcg gtctggtggc gctttatcca ctggtctggt
    39601 tgtgtgcgct ggtcggcacg gtggcgctgt tttacaccgg ctacctgctt taccttggta
    39661 ttccgagctt cctgaatatc aacaaagagg aaggccttag cttctccagt tcgacgctgg
    39721 cgattggcgt gctggtactg gaagtgctgc tcaccctaac cgttatacta tggggctacg
    39781 gttatcgttt gttctagaaa gttctttgac gttgcattgc tggcgtaaat gtcagcaatg
    39841 cagcatttac tcacgattct cctttgacga tcgcactttt tgctcgttat gatggcgtcg
    39901 ctagcctcag taaatcctta ctccggcggt gcgcaacccg tgcgcgtgaa ccactatctg
    39961 aatgctcatc atgccgaaat ttcgagtttc tttatttagc ttggccctga tgctggctgt
    40021 gccttttgca ccgcaggccg ttgcgaaaac ggcagccgct accaccgctt cacaaccgga
    40081 aattgcctcc ggtagcgcga tgattgttga tctgaatacc aacaaagtga tctattcgaa
    40141 ccacccggat ctggtgcgtc cgattgcgtc tatcagcaaa ttaatgaccg cgatggttgt
    40201 gctggatgca cgactgccgc tggatgaaaa actaaaagtg gatatcagcc agacgccgga
    40261 gatgaaaggg gtctattcgc gcgtacgact gaatagcgaa atcagccgta aagatatgct
    40321 gttgctggcg ctgatgtctt cagaaaaccg cgcggcggca agccttgcgc accattatcc
    40381 cggtggttac aaagccttta ttaaggcaat gaatgcgaaa gcgaaatcgc tcggaatgaa
    40441 caacacgcgc tttgttgaac ctaccggatt gtcggtgcat aacgtttcaa ctgcccgtga
    40501 cttaaccaaa ctgctcattg ccagcaaaca atatccgttg atcgggcagt taagtactac
    40561 ccgggaagat atggcaacct tctctaaccc gacgtatacg ctgccgttcc gcaatactaa
    40621 tcatctggtg tatcgcgata actggaatat tcagttaacc aaaaccggct ttaccaatgc
    40681 ggcgggccat tgtctggtga tgcgtacggt tatcaataat aaaccggtgg cgctggtagt
    40741 gatggacgcg tttggcaaat atacccattt tgccgatgcc agccgcctgc gtacctggat
    40801 tgaaaccggt aaagtgatgc ctgtgccggc agcagcgttg agctataaaa aacaaaaagc
    40861 cgcccaaatg gcggcggcgg ggcagacggc acagaacgat taagtgaatt cggatggcga
    40921 tactctgcca tccgtaattt ttactccact tcctgccagt ttttccgttt actggtttta
    40981 ccgatccccg gattcatgct attggtcgga tcattctcgc gatagaactt ctgcaacgtc
    41041 tccggtgctt tatacaaatg accgacgtta tgctcggcag ggtactgcgc gccgcgctgc
    41101 tgtagcagtt ccagcatctg ttcttttaac gcatgcacat ccacgccttt tttcactatg
    41161 taatcctgat ggaagacata gcacataaaa tggccgtaat agagcttgtg caccagctgg
    41221 ctgtcgatct ccggcggtaa atgctcatac cactcggtgt cgttacgccg cagagcgata
    41281 tccagtgcca gaatgtcttc gacttcatcg gaatgcaccg cctgataacg aattgctgcg
    41341 cccgcagcgg cgaaacggtg taaaaacgct ttgctacctt cctccggcgt acagacaaag
    41401 aaatcgccct cggcctgttt gaaataatcc accagccacg atttggcttc accgacgcca
    41461 tcgcctgcca tttttaacag cagatgatgc tcgtatttat cgcgccagtt tttcatgcgc
    41521 ggcggtaaat ggctggggaa caggtgaccg aatttttgca ttgcacggtc agtaaaatgc
    41581 ggacggaaga atttcacttt ctccagcatc gcatcggtgc gtcccttgag attaaagaag
    41641 aacggcatct tgtcggtgcc taacttatca atcatcagga aggtgtcttt gccgtatttt
    41701 tccgcaatat cgtagatatc ccggtgcata tattccccgg caaccggcag attttcgaag
    41761 ttagccagaa tatgacggcg gatttcggtc agcacttccg gctggttggt gccgatataa
    41821 aacacctgct gatttttttc cgcttcgaag gtatcaagac gtaccgcaaa gacggccagc
    41881 ttcccggcgc aaccagaaga ttcaaataac cgatccggat cggcgttata acgtgcgggc
    41941 gtgtcggctt caatatcacg aacgcggtgg acatagtcat aatcgtgggc gtgacgacca
    42001 tcgtgacgca catcgtcatc tttgatgcga tcgtcatcca gcttgctaag gatctgctcc
    42061 ggcgtttcgc ccagatcaat ccccagatgg ttcaccagcg tcagtttgcc atcttcatct
    42121 atacgcgcga ataacgacat ttcggtatac gccgggccac gttgcaccag cgagccgccg
    42181 gagttgttac aaataccgcc gatgaccgat gcgcctatac acgatgatcc aatcaccgaa
    42241 tgcggttcgc gtcccagcgg tttgagggct ttttccagcg aatagagcgt ggtgcccgga
    42301 taggccagca cctgttcgcc cttgccgaga acgtgcagct tgtcgagacg cagggtgctg
    42361 atgatcacga tatcgcgatc atagtcgtta ccgtttggcg tcgatccttc ggtcaggcct
    42421 gtattggcag cctgcatcag aataattttg tcggcggtga cgcaggcttt cagcacccgc
    42481 cacaattcca gtagtgagcc agggaaaacg acagccagcg cgtcgccctg accagaacgg
    42541 aagcccttgc gatagcgggc cgtttttgcg ggatcggtga gcaggtgtga atgaccgacc
    42601 agacgggcaa gttcattcaa aaaggcttta ttatcagttg ttgtcatgga agacattttc
    42661 cactccttgt ggtggcgaaa aaatatcaca aactagaata gcgccttgta tgacaattca
    42721 gcgagtgaat attttgaaaa ttcagacaat aaactgagca ttctttttct ctctccatca
    42781 tgcttatggc aaactacggt ttttgcacca ttttcgctgc cacgtcgggc aacaaaggaa
    42841 gaaaaatcca tatgaaatgg ctatgttcag taggaatcgc ggtgagtctg gtcctgcagc
    42901 cagcactggc ggatgattta ttcggtaacc atccattaac gcctgaagcg cgggatgcgt
    42961 tcgtcaccga tctgctcaag aaaatgacgg ttgatgagaa aattggtcag ctgcgcttaa
    43021 tcagcgtagg cccggataat ccgaaagagg cgatccgcga gatgatcaaa gacggtcagg
    43081 ttggggcgat tttcaacacc gtaacccgtc aggatatccg cgccatgcag gatcaggtga
    43141 tggaattaag ccgcctgaaa attcctcttt tctttgctta cgacgtgctg cacggtcagc
    43201 gcacggtgtt cccgattagc ctcggtctgg cctcgtcttt taacctcgat gcggtgaaaa
    43261 cggtcgggcg tgtctctgct tatgaagcgg cagatgatgg cctgaatatg acctgggcac
    43321 cgatggtcga tgtctcgcgc gatccgcgct ggggacgtgc ttccgaaggt tttggcgaag
    43381 atacgtatct cacctcaata atgggtaaaa ccatggtgga agcgatgcag ggtaaaagcc
    43441 cggcagatcg ttactcggtg atgaccagcg tcaaacactt tgccgcatac ggcgcggtag
    43501 aaggcggtaa agagtacaac accgtcgata tgagtccgca gcgcctgttt aatgattata
    43561 tgccgccgta caaagcgggg ctggacgcag gcagcggcgc ggtgatggtg gcgctgaact
    43621 cgctgaacgg cacgccagcc acctccgatt cctggctgct gaaagatgtt ctgcgcgacc
    43681 agtggggctt taaaggcatc accgtttccg atcacggtgc aatcaaagag ctgattaaac
    43741 atggcacggc ggcagacccg gaagatgcgg tgcgcgtggc gctgaaatcc ggaatcaaca
    43801 tgagtatgag cgacgagtac tactcgaagt atctgcctgg gttgattaaa tccggcaaag
    43861 tgaccatggc agagctggac gatgctgccc gccatgtact gaacgttaaa tatgatatgg
    43921 ggttgtttaa cgacccatac agccatttgg ggccgaaaga gtctgacccg gtggatacca
    43981 atgccgaaag ccgcctgcac cgtaaagaag cgcgtgaagt ggcgcgcgaa agcctggtgt
    44041 tgctgaaaaa ccgtctcgaa acgttaccgc tgaaaaaatc ggccaccatt gcggtggttg
    44101 ggccactggc ggacagtaaa cgtgacgtga tgggcagctg gtccgcagcc ggtgttgccg
    44161 atcaatccgt gaccgtactg accgggatta aaaattccgt cggtgaaaac ggtaaagtgc
    44221 tgtatgccaa aggggcgaac gttaccagtg acaaaggcat tatcgatttc ctgaatcagt
    44281 atgaagaagc ggtcaaagtc gatccgcgtt cgccgcaaga gatgattgat gaagcggtgc
    44341 agacggcgaa acaatctgat gtggtggtgg ctgtagtcgg tgaagcacag gggatggcgc
    44401 acgaagcctc cagccggacc gatatcacta ttccgcaagg ccaacgtgac ttgattgcgg
    44461 cgctgaaagc caccggtaaa ccgctggtgc tggtgctgat gaacgggcgt ccgctggcgc
    44521 tggtgaaaga agatcagcag gctgatgcga ttctggaaac ctggtttgcg gggactgaag
    44581 gcggtaatgc aattgccgat gtattgtttg gcgattacaa cccgtccggc aagctgccaa
    44641 tttccttccc gcgttctgtc gggcagatcc cggtgtacta cagccatctg aacaccggtc
    44701 gtccgtataa tgccgacaag ccgaacaaat atacttcgcg ttattttgat gaagctaacg
    44761 gagcgcttta tccgttcggc tatggtctga gctataccac tttcaccgtc tctgatgtga
    44821 aactttctgc gccgaccatg aagcgtgacg gcaaagtgac ggccagcgtg caggtgacga
    44881 acaccggtaa gcgcgagggg gccacggtag tgcagatgta cttgcaggat gtgacggctt
    44941 ccatgagtcg ccctgtgaaa cagctgaaag gctttgagaa aatcaccctg aaaccgggcg
    45001 aaactcagac tgtcagcttc ccgatcgata ttgaggcgct gaagttctgg aatcaacaga
    45061 tgaaatatga cgccgagcct ggcaagttca atgtatttat cggcactgat tccgcacgcg
    45121 ttaagaaagg cgagtttgag ttgctgtaat gctttagtaa gggcgcgacg tttgcgccct
    45181 ttgcctgatg ttcatatctt aacttgctga attttcgccc tttgtaggcc ggataaggcg
    45241 ctcgcgccgc atccggcagg aaacgccgga ccgaatatgc ctcttaagta tttatctgat
    45301 tctgcaactg aatccttccg ctcaggctaa ctctgtcatt atcaactatg cttttctctt
    45361 aattcgctga aaacaacagt aaaagaatga ggaaagcatc atgccactct taaagctctg
    45421 ggcaggttca ctggttatgt tggcagccgt gagcctgccg ctacaggcgg cttcccccgt
    45481 taaagtcggt tcaaaaatcg ataccgaagg cgcgctgctc ggcaacatca tcttgcaggt
    45541 actcgaaagc cacggcgtac cgacggtgaa taaagtgcaa ctcggaacga ctcctgtggg
    45601 gcgcggggcg attacttccg atgaactgga tatctatccg gaatataccg gcaatggcgc
    45661 tttcttcttt aaagatgaaa acgatgcagc gtggaaaaat gccgggcagg gctacgagaa
    45721 agtcaaaaag ctcgatgcgg agcacaacaa gttaatctgg ctgacgcccg cgcctgcaaa
    45781 taacacctgg actatcgccg tgcgtcagga tgtggcagag aaaaacaaac tcacttcgct
    45841 tgccgacctg agtcgttatc tgcaagaggg cggcaccttc aaactggcag cctcggcaga
    45901 gtttatcgaa cgcgccgatg cgttacccac gtttgaaaaa gcctatggct ttaagctcgg
    45961 tcaggatcag ttgctgtcac tggctggtgg cgacacggcg gtgacgatta aagccgctgc
    46021 ccagcaaacc tctggcgtta atgccgcaat ggcttacggc actgacggtc cggtcgcggc
    46081 gctggggctg caaaccttaa gcgatccgca aggcgtgcaa cctatctacg cgcctgcacc
    46141 agtggtgcgt gaatcggtgt tgaaggagta tccgcaaatg gcacagtggc tacagccagt
    46201 cttcgccagc ctcgatgcaa aaacattgca gcaactgaat gccagcattg cagtggaagg
    46261 actggatgcc aaaaaagtgg ctgccgacta cctgaaacaa aaagggtggg cgaagtaatt
    46321 tcccgtgact tatctacgta ttaatcctgt tctggcgctg ctgttgttgc tgacggcaat
    46381 cgcagcggcg ctgccgttta tcagttacgc gcctaatcgt ttagtctcgg gtgaagggct
    46441 tcatctctgg caactgtggc cgcaaacgat ctggatgctg gtgggcgttg gttgcgcctg
    46501 gctgacagcc tgttttgtcc ccgctaaaaa aggcagcatt tttgcactca ttctggcgca
    46561 attcgtcttc gtattgttgg tgtggggagc tggaaaggcg gcgacccaac tggcgcaaaa
    46621 tggcagtgcg ctggcgcgta ccagcctcgg cagaggtttc tggctggctg cggcgctggc
    46681 attgctggcc tgtagcgatg ccatccgccg aatctccacg catccgctgt ggcgctggtt
    46741 gttgcatatg cagattgcca ttattccgct gtggttgctg tactccggca cgcttaacga
    46801 tctctcacta atgaaagaat acgccaaccg tcaggatgtg tttgacgacg cgctggcaca
    46861 acatctgacg ttgctgtttg gtgcggtgct gcctgcgtta gtgattggtg tgccgttggg
    46921 catctggtgc tacttttcca ctgcgcggca gggggcaatt ttttctctgc tcaatgtcat
    46981 tcagaccgtg ccttcggtgg cgctctttgg cctgttgatt gcgccgcttg ccgcgctggt
    47041 tacggccttt ccgtggctgg ggaagctcgg catagcagga accggaatga cacccgcact
    47101 gattgcgctg gtgctctatg ccttgctgcc gctggtgcga ggcgtggtag tcggtttgaa
    47161 ccagatcccg cgcgatgtgc tggagagcgc cagagcgatg gggatgagcg gggcgcagcg
    47221 tttcctgcat gttcagttac cgctggcgtt accggtattt ctgcgcagcc tgcgggtggt
    47281 gatggtgcaa actgtaggta tggcggtgat tgcggcgtta atcggcgcag gcggttttgg
    47341 tgcgctggtt ttccaggggc tgctaagcag cgccattgat ttagtgttgc tgggggtgat
    47401 cccggtaatt gttctggcgg tgcttaccga cgcgctgttc gatttgctta tcgcactgct
    47461 gaaggtgaaa cgtaatgatt gaatttagcc atgtcagcaa actgttcggc gcacaaaaag
    47521 ccgttaacga tctcaatctc aattttcagg aagggagttt ttcggtgctg attggcacat
    47581 ctggctccgg caaatccacc accctgaaaa tgattaaccg cctggtggag catgacagcg
    47641 gagtgatccg ctttgccgga gaagaaattc gctcgctgcc agtactggag ttgcgccgcc
    47701 ggatgggcta tgccattcaa tctattggcc tgttccccca ctggagcgtg gcgcaaaaca
    47761 tcgctaccgt gccgcaatta caaaaatggt cgcgggcgcg gattgacgat cgtatcgacg
    47821 aattaatggc gctactgggg ctggagtcaa atttgcgtga gcgttatccg catcagcttt
    47881 ccggtggtca gcagcaacgt gtgggagtgg cgcgtgcact ggctgccgat ccgcaagtct
    47941 tactgatgga tgaacctttt ggcgcactgg acccggtaac gcgcggcgcg ttgcaacaag
    48001 agatgacgcg cattcaccgt ttgctggggc gcactattgt gctggtcact catgatattg
    48061 atgaggcgct acggctggca gaacatctgg tattgatgga tcacggtgaa gtggtgcagc
    48121 aggggaatcc gctgacgatg ctgactcgtc cggcgaatga ttttgtccgc cagttttttg
    48181 gacgtagtga actgggggtg cacctgcttt cgttacgtag cgtggcggat tacgtgcgtc
    48241 gcgaagaacg ggcagaaggt gaggcactgg cagaagagat gacgctacgc gatgcgctct
    48301 ccctgtttgt cgcgcgggga tgcgaggtgc tgccggtggt gaacacgcag ggccagcctt
    48361 gcggcacgct gcattttcag gatctgctgg tggaggcgta agcgtatgaa gatgttgcgc
    48421 gatccgctgt tctggctcat tgctcttttt gtggcgctga ttttctggct gccttacagc
    48481 cagccgctgt ttgctgcctt gttcccacaa ctgccacgac ccgtttatca gcaagaaagt
    48541 tttgcagctc tggcactggc tcatttctgg ctggtgggaa tttcgagttt gtttgcggtg
    48601 atcattggca caggtgccgg aattgctgtt actcgcccgt ggggcgcgga atttcgccca
    48661 ctggtggaaa ctattgccgc cgttggacag acttttccgc ctgtcgcagt gctggcgatt
    48721 gccgttccgg tgatcggctt tggtctgcaa ccagcgatta tcgccttgat cctttacggt
    48781 gtgctgccca tcctgcaggt gacacttgcc gggctgggag cgattgatcc cagcgtgaca
    48841 gaagttgcga aaggtatggg aatgagtcga ggtcagcgac tgcgtaaggt cgagctaccg
    48901 ctggcggctc cggtgattct ggcgggcgtg cgaacttcgg tgattatcaa cattggtacg
    48961 gcgacgatcg cctcaacggt aggggccagt acgctgggta cgcccatcat catcgggctt
    49021 agcggattta ataccgcgta tgtgatccag ggggcgttac tggtggcact ggcggcgatc
    49081 atcgcagacc gcctgtttga aaggctggtg caggcgctta gccagcacgc aaaataaagg
    49141 tataacctgc gagcatgacg ccaccaattc cgcctaacgc cataaacagg aacagggcga
    49201 tgaccccaat tttagctatg cgcataatgc actccttatg ttaacgaaag gattgtacag
    49261 taaagcgcat ttgttaacga atcattaaat accgagtggg aaaatatcat ggccttgttc
    49321 ttgccaactg gtgagttgct gctgttgggc ggaggttcga ttttcaccgc accacaccag
    49381 caatgtacgg ccttcgaata gttcagggcg tagttgattg agcgagtggg cgagcgagga
    49441 catcaatgcg ccatccttgt tgactggcaa tccagccctc cagccacaga cgggtggtat
    49501 cctgaatatt ccagccaacc accagcgcat ctttaccctg ttttttacgt gccgaagcca
    49561 gacaaatggc gatgtagttg atcagtacgc cgtcgaggat cgccagcagc gcctggagag
    49621 tcggttgttg gcactgaagc cgtcggcgca gaggaataaa cagatgtgtg gtgagtgtct
    49681 gggcggggta atcctgaccg cgctctttga tccacgttcg caggctatgt agattgc
//
LOCUS       DAZSNG010000019        48321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZSNG010000019 DAZSNG010000000
VERSION     DAZSNG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.019"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACHW4Z_001153"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACHW4Z_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR8880882.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHW4Z_001154"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHW4Z_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR8880883.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="ACHW4Z_001155"
     CDS             complement(1922..2374)
                     /locus_tag="ACHW4Z_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR8880884.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHW4Z_001156"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHW4Z_001156"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR8880885.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="ACHW4Z_001157"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="ACHW4Z_001157"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR8880886.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHW4Z_001158"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHW4Z_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR8880887.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHW4Z_001159"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHW4Z_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR8880888.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHW4Z_001160"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHW4Z_001160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR8880889.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHW4Z_001161"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHW4Z_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR8880890.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHW4Z_001162"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHW4Z_001162"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8880891.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHW4Z_001163"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHW4Z_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR8880892.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHW4Z_001164"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHW4Z_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR8880893.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="ACHW4Z_001165"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="ACHW4Z_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR8880894.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHW4Z_001166"
     CDS             complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHW4Z_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR8880895.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="ACHW4Z_001167"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="ACHW4Z_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR8880896.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="ACHW4Z_001168"
     CDS             complement(15085..15582)
                     /locus_tag="ACHW4Z_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR8880897.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="ACHW4Z_001169"
     CDS             complement(15579..15941)
                     /locus_tag="ACHW4Z_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR8880898.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="ACHW4Z_001170"
     CDS             complement(15931..16278)
                     /locus_tag="ACHW4Z_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR8880899.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /gene="yidI"
                     /locus_tag="ACHW4Z_001171"
     CDS             16387..16800
                     /gene="yidI"
                     /locus_tag="ACHW4Z_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR8880900.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="ACHW4Z_001172"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="ACHW4Z_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="ACHW4Z_001173"
     CDS             complement(18324..20039)
                     /locus_tag="ACHW4Z_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR8880901.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="ACHW4Z_001174"
     CDS             20206..21099
                     /locus_tag="ACHW4Z_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR8880902.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="ACHW4Z_001175"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="ACHW4Z_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="ACHW4Z_001176"
     CDS             complement(21910..23526)
                     /locus_tag="ACHW4Z_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8880903.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="ACHW4Z_001177"
     CDS             23821..24495
                     /locus_tag="ACHW4Z_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8880904.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="ACHW4Z_001178"
     CDS             complement(24534..26195)
                     /locus_tag="ACHW4Z_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR8880905.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACHW4Z_001179"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACHW4Z_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR8880906.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACHW4Z_001180"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACHW4Z_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR8880907.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="ACHW4Z_001181"
     CDS             27649..27981
                     /locus_tag="ACHW4Z_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR8880908.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="ACHW4Z_001182"
     CDS             complement(27983..29215)
                     /locus_tag="ACHW4Z_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR8880909.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="ACHW4Z_001183"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="ACHW4Z_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR8880910.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="ACHW4Z_001184"
     CDS             complement(30377..31714)
                     /locus_tag="ACHW4Z_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8880911.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACHW4Z_001185"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACHW4Z_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACHW4Z_001186"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACHW4Z_001186"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR8880912.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACHW4Z_001187"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACHW4Z_001187"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR8880913.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACHW4Z_001188"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACHW4Z_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR8880914.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /gene="yidX"
                     /locus_tag="ACHW4Z_001189"
     CDS             35377..36033
                     /gene="yidX"
                     /locus_tag="ACHW4Z_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR8880915.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACHW4Z_001190"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACHW4Z_001190"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR8880916.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="ACHW4Z_001191"
     CDS             complement(37005..37403)
                     /locus_tag="ACHW4Z_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR8880917.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACHW4Z_001192"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACHW4Z_001192"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR8880918.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACHW4Z_001193"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACHW4Z_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR8880919.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACHW4Z_001194"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACHW4Z_001194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR8880920.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACHW4Z_001195"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACHW4Z_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR8880921.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="ACHW4Z_001196"
     CDS             43964..44044
                     /locus_tag="ACHW4Z_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880922.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="ACHW4Z_001197"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="ACHW4Z_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR8880923.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="ACHW4Z_001198"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="ACHW4Z_001198"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR8880924.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="ACHW4Z_001199"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="ACHW4Z_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR8880925.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="ACHW4Z_001200"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="ACHW4Z_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR8880926.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="ACHW4Z_001201"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="ACHW4Z_001201"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR8880927.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12218 g        11058 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagctcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DAZSNG010000020        48062 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZSNG010000020 DAZSNG010000000
VERSION     DAZSNG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48062
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.020"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACHW4Z_001202"
     CDS             complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACHW4Z_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR8880928.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACHW4Z_001203"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACHW4Z_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR8880929.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="ACHW4Z_001204"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="ACHW4Z_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR8880930.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="ACHW4Z_001205"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="ACHW4Z_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR8880931.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACHW4Z_001206"
     CDS             complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACHW4Z_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR8880932.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACHW4Z_001207"
     CDS             complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACHW4Z_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR8880933.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9762..10139
                     /locus_tag="ACHW4Z_001208"
     CDS             9762..10139
                     /locus_tag="ACHW4Z_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR8880934.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10292..10843
                     /gene="apt"
                     /locus_tag="ACHW4Z_001209"
     CDS             10292..10843
                     /gene="apt"
                     /locus_tag="ACHW4Z_001209"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR8880935.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10972..12897
                     /gene="dnaX"
                     /locus_tag="ACHW4Z_001210"
     CDS             10972..12897
                     /gene="dnaX"
                     /locus_tag="ACHW4Z_001210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR8880936.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12230..12294
                     /gene="dnaX"
                     /locus_tag="ACHW4Z_001210"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12950..13279
                     /locus_tag="ACHW4Z_001211"
     CDS             12950..13279
                     /locus_tag="ACHW4Z_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR8880937.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13279..13884
                     /gene="recR"
                     /locus_tag="ACHW4Z_001212"
     CDS             13279..13884
                     /gene="recR"
                     /locus_tag="ACHW4Z_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR8880938.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13994..15868
                     /gene="htpG"
                     /locus_tag="ACHW4Z_001213"
     CDS             13994..15868
                     /gene="htpG"
                     /locus_tag="ACHW4Z_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR8880939.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16049..16693
                     /gene="adk"
                     /locus_tag="ACHW4Z_001214"
     CDS             16049..16693
                     /gene="adk"
                     /locus_tag="ACHW4Z_001214"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR8880940.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16825..17787
                     /gene="hemH"
                     /locus_tag="ACHW4Z_001215"
     CDS             16825..17787
                     /gene="hemH"
                     /locus_tag="ACHW4Z_001215"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR8880941.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACHW4Z_001216"
                     /pseudo
     CDS             complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACHW4Z_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18895..20199
                     /gene="gsk"
                     /locus_tag="ACHW4Z_001217"
     CDS             18895..20199
                     /gene="gsk"
                     /locus_tag="ACHW4Z_001217"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR8880942.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACHW4Z_001218"
     CDS             complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACHW4Z_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR8880943.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22246..23466)
                     /gene="fsr"
                     /locus_tag="ACHW4Z_001219"
     CDS             complement(22246..23466)
                     /gene="fsr"
                     /locus_tag="ACHW4Z_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR8880944.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23684..25336
                     /gene="ushA"
                     /locus_tag="ACHW4Z_001220"
     CDS             23684..25336
                     /gene="ushA"
                     /locus_tag="ACHW4Z_001220"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR8880945.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25373..25852)
                     /gene="ybaK"
                     /locus_tag="ACHW4Z_001221"
     CDS             complement(25373..25852)
                     /gene="ybaK"
                     /locus_tag="ACHW4Z_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR8880946.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26056..26850)
                     /locus_tag="ACHW4Z_001222"
     CDS             complement(26056..26850)
                     /locus_tag="ACHW4Z_001222"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR8880947.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26988..27329
                     /locus_tag="ACHW4Z_001223"
     CDS             26988..27329
                     /locus_tag="ACHW4Z_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8880948.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27544..30048)
                     /gene="copA"
                     /locus_tag="ACHW4Z_001224"
     CDS             complement(27544..30048)
                     /gene="copA"
                     /locus_tag="ACHW4Z_001224"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR8880949.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30310..31242
                     /gene="glsA"
                     /locus_tag="ACHW4Z_001225"
     CDS             30310..31242
                     /gene="glsA"
                     /locus_tag="ACHW4Z_001225"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR8880950.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31245..32537
                     /locus_tag="ACHW4Z_001226"
     CDS             31245..32537
                     /locus_tag="ACHW4Z_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR8880951.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32568..32665
                     /locus_tag="ACHW4Z_001227"
                     /pseudo
     CDS             32568..32665
                     /locus_tag="ACHW4Z_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32662..33069
                     /gene="cueR"
                     /locus_tag="ACHW4Z_001228"
     CDS             32662..33069
                     /gene="cueR"
                     /locus_tag="ACHW4Z_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR8880952.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33070..33528)
                     /locus_tag="ACHW4Z_001229"
     CDS             complement(33070..33528)
                     /locus_tag="ACHW4Z_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR8880953.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33525..34442)
                     /locus_tag="ACHW4Z_001230"
     CDS             complement(33525..34442)
                     /locus_tag="ACHW4Z_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8880954.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34588..35265
                     /gene="fetA"
                     /locus_tag="ACHW4Z_001231"
     CDS             34588..35265
                     /gene="fetA"
                     /locus_tag="ACHW4Z_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR8880955.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35252..36031
                     /gene="fetB"
                     /locus_tag="ACHW4Z_001232"
     CDS             35252..36031
                     /gene="fetB"
                     /locus_tag="ACHW4Z_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR8880956.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36094..36948)
                     /gene="cnoX"
                     /locus_tag="ACHW4Z_001233"
     CDS             complement(36094..36948)
                     /gene="cnoX"
                     /locus_tag="ACHW4Z_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR8880957.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37009..37818)
                     /gene="ybbO"
                     /locus_tag="ACHW4Z_001234"
     CDS             complement(37009..37818)
                     /gene="ybbO"
                     /locus_tag="ACHW4Z_001234"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8880958.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(37808..38430)
                     /gene="tesA"
                     /locus_tag="ACHW4Z_001235"
                     /pseudo
     CDS             complement(37808..38430)
                     /gene="tesA"
                     /locus_tag="ACHW4Z_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            38401..39087
                     /gene="ybbA"
                     /locus_tag="ACHW4Z_001236"
     CDS             38401..39087
                     /gene="ybbA"
                     /locus_tag="ACHW4Z_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR8880959.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39084..41499
                     /gene="ybbP"
                     /locus_tag="ACHW4Z_001237"
                     /pseudo
     CDS             39084..41499
                     /gene="ybbP"
                     /locus_tag="ACHW4Z_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(41717..41872)
                     /gene="dinQ"
                     /locus_tag="ACHW4Z_001238"
     CDS             complement(41717..41872)
                     /gene="dinQ"
                     /locus_tag="ACHW4Z_001238"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8880960.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(42212..43306)
                     /gene="mnmH"
                     /locus_tag="ACHW4Z_001239"
     CDS             complement(42212..43306)
                     /gene="mnmH"
                     /locus_tag="ACHW4Z_001239"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR8880961.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(43375..44301)
                     /gene="allS"
                     /locus_tag="ACHW4Z_001240"
     CDS             complement(43375..44301)
                     /gene="allS"
                     /locus_tag="ACHW4Z_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR8880962.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            44531..45013
                     /gene="allA"
                     /locus_tag="ACHW4Z_001241"
     CDS             44531..45013
                     /gene="allA"
                     /locus_tag="ACHW4Z_001241"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR8880963.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45091..45906
                     /gene="allR"
                     /locus_tag="ACHW4Z_001242"
     CDS             45091..45906
                     /gene="allR"
                     /locus_tag="ACHW4Z_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR8880964.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            45996..47777
                     /gene="gcl"
                     /locus_tag="ACHW4Z_001243"
     CDS             45996..47777
                     /gene="gcl"
                     /locus_tag="ACHW4Z_001243"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR8880965.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47790..>48062
                     /locus_tag="ACHW4Z_001244"
     CDS             47790..>48062
                     /locus_tag="ACHW4Z_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8880966.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        11432 a        12507 c        12792 g        11331 t
ORIGIN      
        1 ttttatgaat tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt
       61 caattactcc ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg
      121 attacatgtt gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga
      361 tcggacgtaa acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa
      481 tggttgtgag cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc
      601 agctctcgta cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc
     1021 cccatgcagc gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcagcat atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca
     1621 gataggccat gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt
     2041 tttctaccac aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca
     2221 taaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg
     2401 tcgccagctt gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg
     2641 caacctgggc gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt
     2701 tgttcagctc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 catcacccac gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca
     3541 tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg
     3841 ctggactggg tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcacagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta
     3961 atcggagagg tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc
     4081 ttactgatgt actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacctg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta
     5281 acgaataacc ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg
     5521 tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac
     5881 agccagctgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     6001 ttacgtccca gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt
     6061 gaccagcagc gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag
     6421 attgccgtcc tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg
     6481 ctgccctcgg cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg
     6661 gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg
     6721 atggccatta acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa
     6781 tccatcctga ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg
     6841 atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6901 tgggaaaaag cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct
     7021 tccgctgtgg gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg
     7141 accatgcagc tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac
     7261 ttcggcatgc cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc
     7321 gcgttgctgc ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta
     7441 tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc
     7501 attaccgtgc tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg
     7621 aacttgctct accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc
     7861 gtgttcagct atctcgacag catcacgctc tggcattaca acggcactga agctggcgct
     7921 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg
     8041 aatatgcgcc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg
     8161 gccgcagcat tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt
     8281 agcttctcgg ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc
     8641 gatcagctgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg
     8761 acaccagcgg ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac
     8821 aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc
     8881 aagaattcaa tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt
     8941 ttgcgtttgc ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg
     9001 cctcatcatc gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca
     9061 gcacggtact ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt
     9121 tgacatcgtc aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc
     9181 gctagcctgt tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag
     9241 gcttccactt cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg
     9301 gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt
     9361 ttacgctgcc agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg
     9421 gaggcttcac gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact
     9481 tgcggcagct gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg
     9541 tcgagacaag actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca
     9601 cttagcgtgg cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct
     9661 tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg
     9721 ttatgctaaa gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa
     9781 tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac
     9841 cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc
     9901 acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc
     9961 atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca
    10021 tttctctgtg gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct
    10081 gtttgctttt ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa
    10141 gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca
    10201 caggcgcagg cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt
    10261 gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta
    10321 tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga
    10381 tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga
    10441 gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt
    10501 tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa
    10561 actgccgcgt gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga
    10621 aatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc
    10681 aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc
    10741 tgacgctgcg ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca
    10801 gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg
    10861 ctgcccacgc tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt
    10921 gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat
    10981 caggttttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat
    11041 gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt
    11101 tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac
    11161 tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag
    11221 caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat
    11281 acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat
    11341 ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt
    11401 gaagagccgc cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg
    11461 gtgacgattt tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt
    11521 cgccatcagc ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg
    11581 caattgttgg cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag
    11641 gcgattgcca gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg
    11701 cttgacgacg atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc
    11761 gtaatggcac tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg
    11821 gaaatgctcg gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc
    11881 aacgacatgg ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg
    11941 gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg
    12001 gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg
    12061 ccgctgcctg agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta
    12121 atgacgccaa ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc
    12181 ccggaaacca ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca
    12241 accaaagcaa aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct
    12301 gcgctggaaa gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg
    12361 ctggaaaaag cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg
    12421 cagcaaaaag aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa
    12481 acgccggaac tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca
    12541 caggtgagcc aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa
    12601 gaggagagcg acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac
    12661 cgcggtgcac agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa
    12721 ctgactatcg ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg
    12781 atatacgaag aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag
    12841 accctgcgtc ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg
    12901 taagcacagc ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa
    12961 aggcggtctg ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat
    13021 gcaggaagag atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt
    13081 gaccatcaac ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga
    13141 cgacaaagag atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat
    13201 tgaagaaacg cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg
    13261 ctttaagatg ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg
    13321 cgctgtctgc cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag
    13381 cgcgatcgta gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc
    13441 ggccactgcg ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat
    13501 ccgcgtcgtc aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc
    13561 attgagcaga cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg
    13621 ctggacggca tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag
    13681 gaaaaaatca ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct
    13741 aactacattg ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc
    13801 gtaccggttg gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc
    13861 gggcgtcata agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa
    13921 ttattctccc ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt
    13981 gaggtagacc tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct
    14041 tctgcacctg atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat
    14101 ctctaacgcc tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct
    14161 gtacgaaggt gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct
    14221 gaccatctcc gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac
    14281 tatcgctaaa tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga
    14341 cagccagctg atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa
    14401 agtgaccgtg cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc
    14461 ggctggcgaa ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat
    14521 caccctgcat ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat
    14581 catcagcaaa tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa
    14641 agatggcgaa accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg
    14701 taacaagtcg gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga
    14761 ctttaacgat ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag
    14821 cctgctgtac atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg
    14881 cctgaaactg tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa
    14941 ctatctgcgc ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg
    15001 tgaaatcctc caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt
    15061 gctgcaaatg ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca
    15121 acagtttggc ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat
    15181 cgccaaactg ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct
    15241 ggaagactac gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga
    15301 cagctatgcg gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga
    15361 agttctgctg ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga
    15421 cggtaaaccg ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga
    15481 agttgatgag agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa
    15541 agctctgctc ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc
    15601 agcgatcgtt tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc
    15661 ggcgggccag aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact
    15721 ggtgaaacgt gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct
    15781 gctggatcag gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg
    15841 tcgtatgaat cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc
    15901 atttttcttt tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct
    15961 caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa
    16021 atcgacacat tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc
    16081 ggggaaaggg actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac
    16141 tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga
    16201 cattatggat gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg
    16261 cattgctcag gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc
    16321 gcaggcagac gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt
    16381 accggacgaa ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg
    16441 tgtttatcac gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga
    16501 agaactgact acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata
    16561 ccatcagatg actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac
    16621 caaatacgcg aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa
    16681 aatcctcggc taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc
    16741 aggcaatacc tctcacagca attagttctt cttcctcact tttccgctac aattatcaac
    16801 aagttgaatc gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac
    16861 ctgggtacgc ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta
    16921 agcgacagac gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg
    16981 attttgccgc tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac
    17041 ggctcgccgc tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg
    17101 gatacgccag tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat
    17161 gaactcctgg cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc
    17221 tgttctacgg tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc
    17281 attccgggga tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg
    17341 gcgaacagcg tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct
    17401 tatcatggca ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc
    17461 acaacgactc gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc
    17521 tttcagtcgc gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa
    17581 atgctcggag aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat
    17641 tgtctggaga cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc
    17701 gggaaaaaat atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg
    17761 gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga
    17821 agagcctcgt cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct
    17881 gggtagagtt tgaactcaca gggctgctga tgcgccgcta acgtctagta aagcagacgg
    17941 ctgtcatcca gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga
    18001 gtgagatcat tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa
    18061 tacgcctctt catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac
    18121 agacgacgag tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca
    18181 attttaccgc aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg
    18241 gcacctgcgg aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc
    18301 tgctggtgga aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct
    18361 tcaggtgaaa gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg
    18421 cgcatgatgc gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa
    18481 aatagcgtcg ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat
    18541 tttgttggaa ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg
    18601 cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt
    18661 aaatccggct gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca
    18721 ggtagtttgt tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac
    18781 cactgccatg aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct
    18841 cccgtcagat tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa
    18901 tttcccggta aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt
    18961 cagcaattcc agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg
    19021 ctggtcgata ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc
    19081 gggcattcac tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag
    19141 aaaaacctga ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac
    19201 tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt
    19261 ggcagttatg cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta
    19321 caaggcgtgg atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt
    19381 acctttgcta tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat
    19441 gtgattgccg gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt
    19501 gaacccatgc cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg
    19561 gtggtgctga cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa
    19621 ttcctcaaag accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc
    19681 ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg
    19741 tgcaccgccg ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt
    19801 aaaacccagc atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc
    19861 cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg
    19921 tacatgggcg ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca
    19981 gcgttgctgc atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc
    20041 aaacataaat tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac
    20101 cgtgtgagct atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag
    20161 cgtgaagaca gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc
    20221 agataaggcg ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg
    20281 cgtcttatca tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt
    20341 caccacttca cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg
    20401 ttcacccatc actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata
    20461 atgggcgcgg gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc
    20521 acccgcttca taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc
    20581 cagttgcata atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg
    20641 cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag
    20701 caatttctcc cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca
    20761 aatatccact gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag
    20821 cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat
    20881 cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc
    20941 cattcccagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat
    21001 ggttaatgcc gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga
    21061 tttaccaaac agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat
    21121 taacggatca aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa
    21181 tggcaacgta tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc
    21241 aaagaacgca ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc
    21301 taacgccagc gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg
    21361 tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat
    21421 caccttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg
    21481 ttccatcatt cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc
    21541 cacaatcaac caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc
    21601 aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc
    21661 ggtcattaac gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc
    21721 tatctgggcg atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga
    21781 aaaatgcaaa ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc
    21841 aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag
    21901 atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag
    21961 cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt
    22021 tcctggtggt tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg
    22081 acaatttcat tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc
    22141 tgcaaaaaaa gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg
    22201 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
    22261 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
    22321 gactaactcg atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc
    22381 catgccgaaa gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag
    22441 ctcctgagcg tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac
    22501 cgttaaaacc cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac
    22561 gccgaggata gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc
    22621 gatcaccgta cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg
    22681 gatagataat ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat
    22741 atagaaatat ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt
    22801 gcgcggcagt ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc
    22861 cgagtaccaa cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa
    22921 ccaggcaacg ttgcctttgc cataaggctc gataatcacc gccgccagca agggtcccag
    22981 ggaactgcca aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc
    23041 ggaagccata cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac
    23101 cagcgccgct gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc
    23161 acttaaggta aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca
    23221 gtagccgacc actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc
    23281 aatctgcata aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag
    23341 cgattggatc atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga
    23401 tgttcgggcc ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat
    23461 tgccatagga aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta
    23521 ttacccgact aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa
    23581 tttctaatct ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgttgcgtc
    23641 atactatttt tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg
    23701 gggcgtggcg ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta
    23761 tgagcaggat aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt
    23821 ttggcgcaat gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat
    23881 ccgcaaagag gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa
    23941 cactggcgtg cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct
    24001 ggtgggctat gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt
    24061 acgccagcag gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag
    24121 tactggcgag cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc
    24181 cgttattggg ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga
    24241 tatcgaattt cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac
    24301 agaaaagcca gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca
    24361 cggctctaac gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc
    24421 gatgatcgtc ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca
    24481 ggtcgattac gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt
    24541 gcaggcgcat gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga
    24601 aatgaaaatg gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga
    24661 agacgggaaa agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat
    24721 ctcgctgtta tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa
    24781 aaccaatggt cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg
    24841 gcggttgatt ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg
    24901 aggcggaatt cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt
    24961 gcagccattc ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta
    25021 cctgaccgcc gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt
    25081 tagctttgtg gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga
    25141 tccggcgaaa acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata
    25201 tccgcgcctt gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct
    25261 gaaagcgtat atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga
    25321 ggtgagctgg cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc
    25381 ggcgggcgat atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt
    25441 cgatatccag tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg
    25501 cgtcgataat cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt
    25561 atcccgtcga acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg
    25621 cttttgctac ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt
    25681 gtttcatatc accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac
    25741 ctaatttttt gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat
    25801 ggatttgaaa cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt
    25861 tcctgcctta aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag
    25921 tcatcttgcc taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt
    25981 cgcttaaagt gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg
    26041 atattggcca tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag
    26101 tgcaccgacc gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc
    26161 gagatttcgc tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt
    26221 tatatcatta ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg
    26281 tgcgttggta tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag
    26341 ctggagcaac atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg
    26401 ttgcttcgcc gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt
    26461 ttgtgcctgc gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag
    26521 tgagggagaa atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct
    26581 aatgcgctct tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga
    26641 aacatccgcc tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg
    26701 caggggagcc atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt
    26761 gccggggagg gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca
    26821 aagcgttttt acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag
    26881 cgcgcggtga gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc
    26941 agtatgttct tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa
    27001 caagaaaacc gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt
    27061 tgaaaatcaa tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga
    27121 tcaataacaa tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata
    27181 ccacagtcga ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca
    27241 acatgcgcac ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc
    27301 gtgaagagcg tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg
    27361 ataaggcgtt cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg
    27421 tcttatcagg cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca
    27481 tccggcagtc atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg
    27541 tacttattcc ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga
    27601 cgagagcgcc attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca
    27661 caaaataccg gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt
    27721 ctgcttcatg ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag
    27781 gctatggcgc atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat
    27841 cgcaatgccg acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat
    27901 tgccacctga cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag
    27961 cactccggca atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg
    28021 gttatccccg gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc
    28081 cacgctatca ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat
    28141 cgccagcagc acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc
    28201 tttagtatcg acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc
    28261 ttctgcttca ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag
    28321 ctgcatatcg cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc
    28381 cagcgccgcc gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc
    28441 gacaacctgc ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag
    28501 tgtactggcg cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg
    28561 cccgacgccg gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat
    28621 aatcagcacc gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa
    28681 ataccagatt gccgcactga caagcgcaat aaccactact accggcacaa atatggctga
    28741 gattttatcc gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg
    28801 aatgatcctt gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc
    28861 gtcctgtacc actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg
    28921 ttcgcccgtc agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac
    28981 cggcacgcga tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg
    29041 cacgcttttt tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa
    29101 tttctccagt gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt
    29161 gatcagaccg ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa
    29221 ccactgcggc cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc
    29281 caccagcgta tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa
    29341 atggccgctg gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct
    29401 gcggttgtcg gcggtgacta tcatgttatc gccgatcatc ccccagacca tcaccgggat
    29461 acccaccgcc agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt
    29521 ttcttgctgg cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc
    29581 tttttccacc gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg
    29641 ctccgccagg tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac
    29701 gcgggtgaca cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc
    29761 atcggcggtc gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga
    29821 tgactccgcc agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt
    29881 gatggtttcg atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga
    29941 cacatccgct tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca
    30001 gtgaccgcag gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt
    30061 taagacagtt ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga
    30121 aattaataaa ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc
    30181 aggattagac atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc
    30241 aggatgcctg aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt
    30301 taacaaaaga tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa
    30361 tttcactcac ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca
    30421 ggtcaactgg cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac
    30481 agtgattacc gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta
    30541 gaagatgtcg gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc
    30601 tttaactcag ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat
    30661 gctggcgcta ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag
    30721 cgaattttac atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc
    30781 aaccagtcgg aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc
    30841 gccggatatc tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct
    30901 acgctcatca atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg
    30961 aatccgttga cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa
    31021 atgatgatgg aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg
    31081 ggcaaaagcg gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc
    31141 gcgttctcac caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca
    31201 tcggtcgcta agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag
    31261 gtaataacgg taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa
    31321 tggtgggggc ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga
    31381 cctggggcgc ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc
    31441 gtctgggggc gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag
    31501 gcaacggcgt cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca
    31561 tcgccatggt tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc
    31621 aggaggagca ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca
    31681 actccttaag caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga
    31741 tgatcctgtt attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg
    31801 tctctgcgcc ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct
    31861 atgcgggctt tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta
    31921 tgccacgggc gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc
    31981 tggttttgct tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag
    32041 cgcaggctgc ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt
    32101 tactggcgac ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca
    32161 acatgggcag cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta
    32221 cctggggcaa tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag
    32281 actcactcgc cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg
    32341 tggcgatccg cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt
    32401 tggtgatgtt gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg
    32461 cgttgatatg gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc
    32521 gtaataaaac cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg
    32581 tcgatattcg cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc
    32641 acaggaggcg ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc
    32701 agcaaagcca ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa
    32761 aacggttatc gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca
    32821 cggcaggtgg gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg
    32881 cagcggcaca gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga
    32941 cacattgagg agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct
    33001 ggcgatgaca gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg
    33061 gcagggtgat taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac
    33121 ttcaacatgc gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc
    33181 gacgcgcata tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat
    33241 cagctgctgc ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg
    33301 cgacaaccat ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc
    33361 ttgccactcc caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac
    33421 gccgctccac aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc
    33481 gagactgagc cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt
    33541 ttgttggcgc tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg
    33601 gcctctaatg gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct
    33661 tcggtgtatt tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct
    33721 tcagacacca ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc
    33781 tgtaaaaacg ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct
    33841 tcggctttga ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg
    33901 gtacgttccg ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg
    33961 tcgcgaattt caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata
    34021 cgcagcaggc gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa
    34081 cccaacacgg tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg
    34141 acttcatagg cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta
    34201 acgttggcgt tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc
    34261 atattgatct tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt
    34321 aacgttttgg tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt
    34381 ttgacgcccg cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc
    34441 atcggaaaaa cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa
    34501 attatacctg attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt
    34561 agtaaaaatc agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa
    34621 aacgtaggat atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt
    34681 gctggcgaat ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa
    34741 atagttgctt cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc
    34801 agcacactaa aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg
    34861 ctgtttggcg atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag
    34921 cctgacccgg ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg
    34981 acgaagaata tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac
    35041 ctgcaattta tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt
    35101 aataaacata acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg
    35161 ctgtgggtga cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa
    35221 ccgcatgccg gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc
    35281 attagcactg gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact
    35341 ggcgctggag aaagatattc tatggagcgt cgggcgagcg ataattcagc tgattattgt
    35401 cggctatgtg ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt
    35461 gttatttatc tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa
    35521 agcttttatc tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct
    35581 gattctctca ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat
    35641 tgccggtaac gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat
    35701 tagcgaacag caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc
    35761 agcgatattg attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa
    35821 aacggttggc ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga
    35881 tccggtgaag gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag
    35941 cttgtcgacc attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca
    36001 gttggtggtg actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc
    36061 ggcgttgtgg ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg
    36121 gcgatacttc gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt
    36181 tttacgcgtc tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc
    36241 cagcgcctct tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc
    36301 ggcatcttct ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc
    36361 cgcctgcttc agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg
    36421 caacggaatg gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt
    36481 ttctgccagc agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag
    36541 caatggcagg gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt
    36601 cagctcttct tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg
    36661 cggcccctgg aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc
    36721 gcgcagacca aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat
    36781 aaattgcccg ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg
    36841 ctggctacgt tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac
    36901 ctgttgcagg ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc
    36961 tgttgtcgat attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa
    37021 tattttgtcc atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac
    37081 cagcgttacc ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc
    37141 gaccaccgct tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt
    37201 atcactttgc gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat
    37261 caggctgact ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc
    37321 cagcgcatat ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac
    37381 cgatgatgtc atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat
    37441 ggtgagctgg tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct
    37501 gatggtggaa agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca
    37561 attatcggtc agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag
    37621 atcgatcaac acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg
    37681 gcaacctgcc agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat
    37741 tccactggaa catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc
    37801 cgttgcttta tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa
    37861 taaacggctg ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga
    37921 ggtagacctc ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg
    37981 ggtaaatggc gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt
    38041 gcattaacaa tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt
    38101 gctcggtttg ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac
    38161 ccaacgcggc tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt
    38221 gtcgccgctg atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa
    38281 ggcaggccag gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat
    38341 caataacgtg tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa
    38401 atgccagcgg aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag
    38461 catgaactct ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca
    38521 ctggtgggcg agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat
    38581 gacggcagca gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa
    38641 gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt
    38701 cctaccctta acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc
    38761 gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg
    38821 gatcatcttc cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc
    38881 tttaatggtc gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag
    38941 acgggcgata aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg
    39001 attatggtga cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg
    39061 aacgggcagt tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc
    39121 cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg
    39181 gcaatatcag cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg
    39241 gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa
    39301 agcgcggcct gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca
    39361 caccgcagct ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcggtctgc
    39421 aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga
    39481 tggcactgct taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga
    39541 ttgccggaga agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc
    39601 gtctgatgat gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg
    39661 tcacctggcg ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt
    39721 tgttacctca gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc
    39781 tggggcgatc gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc
    39841 tgctggcagt ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc
    39901 tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg
    39961 gtcagtggtt gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt
    40021 tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca
    40081 gcctctggcc gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc
    40141 tacgaccata ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg
    40201 tcgcgaacgt ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc
    40261 tgctcgccgg attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag
    40321 tactggcttt gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga
    40381 cgctgaaatc gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa
    40441 cgttaagcca gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc
    40501 tgcgtggcga tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact
    40561 ttttaattaa catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc
    40621 atatagtccc ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaatgata
    40681 agccgacaga aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa
    40741 atacgcggcc cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag
    40801 tgtcgatgga agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt
    40861 ttatgggcga tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg
    40921 aaagtctgcg gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc
    40981 agagctggct taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc
    41041 gccagttccc gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg
    41101 tgctggagca ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta
    41161 tgttgctgtt gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg
    41221 tatggcgcac actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgatgttc
    41281 gccatgcttg ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca
    41341 gtgttgcagg cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt
    41401 ctgccgtgca gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg
    41461 gttaagggta aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg
    41521 atgcgctacg cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg
    41581 taggccggat aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa
    41641 tatgaaacca actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt
    41701 agctacgttt cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg
    41761 ccttaagaac gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag
    41821 aaagacaatc acagcttacc cttacgttgc ctataactat gattttcttc atgtgctaga
    41881 tgaggactgt ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag
    41941 gacttaaaag tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc
    42001 gggattgtat cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt
    42061 gctagatgag gactgtttaa tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa
    42121 aaaaaccgac tgtcatgtcg gtttttttat atctgtattc tggtgcaacc attttgttct
    42181 cacgacaatc gccgtttcag aataccgccc atcactgcgc cttaacccat accgccactt
    42241 ccgcccactc accgcgaaag acaacttttt ccgctttttt ctcaagctga tagcgataca
    42301 ttgggtcgta atattcttta agtaacggca ccagccaggc cagatgaccg tcggtgctgc
    42361 cggtggtgag ttgcgttgtc agcgccgtat ccagctgggc agcgagttcg ttatagcgct
    42421 gtaaccccag ccgacgctta atcgccgaaa gtccgtgatg caggtattcg caatattcct
    42481 gccagccctg ttcgtcgcca taagcgtggg taaaatcatg atgcatacgc aagaaatact
    42541 cttcattcag gcgttcaaga cggatctcaa acggatcttc taccaccgca atcgccgcct
    42601 gagtcattcg ctcgcgcaga cattccggca ggtgattcga accgatcatc cggctttcat
    42661 cttccagcac ccacaggcgc aaatcctgac gggcgtcggt ttttagcatt tcggcagcca
    42721 gcaggttttc aaaactcgcc tggctaagtt gtggttgtaa cgtgcgacca aacgccgaac
    42781 cgcgatgacg cgccagccct tccagatcaa caccgtttgg ctgttgttgc actaatagcg
    42841 ttttaccgct gccggtacaa ccgccaatca gcactatcgg tttttgtgcc agttcaatgg
    42901 tcgcctgaat cgcggtctgg cgcagtgcct tataaccgcc ttccaccagc ggataatcaa
    42961 tccccgcttc atgcagccag cgttgcacaa tatgtgaacg ttgaccgcca cgggcgcagc
    43021 agagaatacc ttgcggattt tgcaggcacg ctgcccgcca ggcgtacata cgctgctgac
    43081 gaatttcacc cgccaccagt ttatgcccca gcgccagcgc tgcgtctgag ccttgctgtt
    43141 tatagcaggt gccaacggag gcgcgttcat cgttatccat taacggcaga ttgatagcgg
    43201 cgggcattgc gccttgctca aactcgatag gggcgcgaac atcaataatg ggcgtatcag
    43261 caatcagcag ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac
    43321 ctcaaatcag cttgcgcgct attttacgcg ccaacgcgca aggaaacttg atttttaact
    43381 gcgtggggtg ccgaaaattt ctaaaaatcc gctgatttcc ggcctgtgtt gggtaaacaa
    43441 ggtcacaata tcttctaccg ctttgcagct gccaaatttg cgccatgcca gactcaatgg
    43501 cgaaggaggg cgcatcgttg ggattacccg gctgaccagt tgttgattat cgatcattga
    43561 ctggcaaagt gattttggca aaaaaccaat gccaacaccc gccagatggg cggcgatttt
    43621 cgtttccata tcgggaacaa taatctcttt ttgccccggc aatcgccagg cgacgcgttt
    43681 ggttaaggtg ccggcgctgt cttcaatatt gaccgccgga aagcgacgca actgcgcttc
    43741 tgttagcggc tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa atcgccattg
    43801 caccgatcct aagggatcaa gactaaaggt atttgccagc gcctcagttc ccgtgacccc
    43861 gatagccagc gaaaaacctt cgtacaatag cgagtcccag acgcccatat agatttgtcg
    43921 ggagatgtga aactgggtaa aggggtaacg ctcattcagc cacgccagca actgggcgac
    43981 ggcctggggg ttatagagca ggttgttgat gacaatattc acctggcgtt ccacgccatc
    44041 attcacctgt tgcagctcgc tgggcatact ttccagccag ctcagccagt ctctggcctg
    44101 ggaaagtaga tgctcgccag ccgctgtcaa cgttacgcta cgagtcgtac ggaaaaacag
    44161 cgctactccg gtattctctt ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt
    44221 acataatcgt tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg
    44281 caaggtttct ggatcgaaca tcttcaggta tcccctttta aatccgcaag ttgcgtgatt
    44341 ttcttatcct ctgatttatc agtattttta catgataatc ctgttcaatt tgtggactaa
    44401 atctagtttt ggaaaaacat tccaactttt gtattaatgt tgttctctta aggttttaga
    44461 ttgcctgtta ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat tatgttacaa
    44521 ggacaaaaag atgaaacttc aggtattacc gttaagtcag gaagccttta gtgcttatgg
    44581 cgacgtaatc gaaacgcagc aacgggattt tttccatatt aacaatggcc tggtggagcg
    44641 ttaccacgat ttggcgctgg ttgagattct tgagcaagac cgtacgctta tcagcattaa
    44701 ccgcgcgcaa ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc atccgctggg
    44761 cattcaggcc tttatcccaa tgaaaggtga agtgtttgtg gtggtcgtgg cgttaggcga
    44821 cgacaaacca gacctgtcaa cgctgcgggc gtttatcacc aacggtgaac agggggtgaa
    44881 ttaccatcgt aacgtctggc atcacccact tttcgcctgg cagcgcgtca ccgattttct
    44941 gaccatcgat cgcggcggca gtgacaactg tgatgttgaa agtattcctg aacaggaact
    45001 ctgttttgcg tgacgcctgc aaccgacttg cataagataa actaattgtt cattgtttat
    45061 gctcacttgt aggtcggagt taacgtaggt atgacggaag ttagacggcg cggcaggcca
    45121 ggacaggcgg agcctgtggc acagaagggc gcacaggcgt tagagcgggg aattgcgatt
    45181 ctgcaatatc tggaaaaaag tgggggaagt tcgtcggtta gcgatatttc tctcaatctg
    45241 gatttgccgc tctccacgac ctttcgcttg ctgaaggttt tacaagcagc ggattttgtc
    45301 tatcaggaca gtcaattagg ctggtggcat ataggattag gtgtctttaa cgtcggtgcg
    45361 gcgtacatcc ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg ccgcctgatg
    45421 ttactttccg gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc ggtattaatt
    45481 ggtcagttag agtgtaaatc gatggtcagg atgtgtgcgc cactgggcag tcgtctgccg
    45541 ctgcatgctt ccggtgcggg caaagcgctg ctttatccgc tggcggaaga ggagttgatg
    45601 agcatcattc tgcaaaccgg tttgcagcag tttacgccaa ctacgcttgt ggatatgccc
    45661 accttgctga aggacctgga acaagcgcgt gaactgggct ataccgtaga taaagaagag
    45721 catgttgtag gtctgaattg catagcttca gcaatttacg atgatgtcgg tagagttgtt
    45781 gccgctatct ccatctccgg gccttcatca agactgacag aagatcgttt tgtcagtcag
    45841 ggtgagctgg tcagagacac cgcccgcgat atcagcacgg cgttgggact gaaagcacat
    45901 ccatagtgtc tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa tttgaaagtt
    45961 ggaaaatttt tccaactaat agaggtagga ataaaatggc aaaaatgaga gccgttgacg
    46021 cggcaatgta tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt ccgggagctg
    46081 caattaatcc gttctactca gcgatgcgta agcacggcgg tattcgtcac attctggcgc
    46141 gtcatgtgga aggtgtttcg cacatggcgg aaggttatac ccgcgcaacg gcagggaata
    46201 tcggcgtatg tctggggact tccggtcctg cgggcacgga catgatcacc gcgctctatt
    46261 ccgcttctgc tgattccatt cctattctgt gcattaccgg ccaggcaccg cgcgcccgtc
    46321 tgcataaaga agattttcag gccgtagata ttgaagcaat tgctaaaccg gtcagcaaaa
    46381 tggcggttac agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag gcatttcacc
    46441 tgatgcgttc tggtcgtccg ggtccggtac tggtggattt accgttcgac gttcaggttg
    46501 cggaaatcga gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa cctgctgcca
    46561 gccgtatgca gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt ccggtgattg
    46621 ttgccggggg cggggtaatc aatgctgacg cagctgcact gttacaacag tttgctgaac
    46681 tgaccagcgt tccggtgatc ccaacgctga tgggctgggg ctgtatcccg gacgatcatg
    46741 aactgatggc cgggatggtc ggtctgcaaa ccgcgcatcg ttacggtaac gcaacgttgc
    46801 tggcgtccga catggtgttt ggtatcggta accgttttgc taaccgtcat accggttcgg
    46861 tagagaaata caccgaaggg cgcaaaatcg ttcatattga tattgagccg acgcaaattg
    46921 gtcgcgtgct gtgtccggat ctcggcattg tctctgatgc taaagcggcg ctgacactgc
    46981 tggttgaagt ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt aaagaatggg
    47041 tcgccgactg ccagcagcgt aaacgcactt tgctgcgcaa aacccacttc gacaacgtgc
    47101 cggtgaaacc gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc gatgtttgtt
    47161 atgtcaccac tattggtctg tcacaaatcg ctgcggcaca aatgctgcat gtctttaaag
    47221 accgccactg gatcaactgt ggtcaggctg gtccgttagg ctggacgatt ccggcggcgc
    47281 tggggggttg tgccgctgat ccgaaacgca atgtggtggc gatttctggc gactttgact
    47341 tccagttcct gattgaagag ttagccgttg gcgcgcagtt caacattccg tacatccatg
    47401 tgctggtgaa taacgcctat ctcggcctga ttcgtcagtc acaacgcgct tttgacatgg
    47461 actactgcgt gcaactcgct ttcgagaata tcaactccag tgaagtgaat ggctacggtg
    47521 ttgaccacgt aaaagtagcg gaaggtttag gttgtaaagc tattcgggtc ttcaaaccgg
    47581 aagatattgc gccagccttt gaacaggcga aagccttaat ggcgcaatat cgggtaccgg
    47641 tagtcgtgga agttattctc gagcgtgtga ccaatatttc gatgggcagc gaactggata
    47701 acgtcatgga atttgaagat atcgccgata acgcagcgga tgcaccgact gaaacctgct
    47761 tcatgcacta tgaataaggg agataaatga tgttacgttt ctctgctaat ttatcgatgt
    47821 tatttggtga atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt ggttttcgcg
    47881 gcgttgaatt tatgtttcct tatgactacg acattgaaga attaaaacag gtactggcga
    47941 gtaataaact cgaacatacg ctgcacaatt taccggcagg tgactgggcg gcgggcgagc
    48001 gcggtattgc ctgtattcct ggccgtgaag aagagtttcg ggatggcgta gcagcagcga
    48061 tt
//
LOCUS       DAZSNG010000021        47956 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZSNG010000021 DAZSNG010000000
VERSION     DAZSNG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.021"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..79
                     /locus_tag="ACHW4Z_001245"
     CDS             <1..79
                     /locus_tag="ACHW4Z_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8880967.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHW4Z_001246"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHW4Z_001246"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR8880968.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHW4Z_001247"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHW4Z_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR8880969.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHW4Z_001248"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHW4Z_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR8880970.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHW4Z_001249"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHW4Z_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR8880971.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHW4Z_001250"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHW4Z_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR8880972.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHW4Z_001251"
     CDS             5377..5835
                     /locus_tag="ACHW4Z_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR8880973.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHW4Z_001252"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHW4Z_001252"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR8880974.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHW4Z_001253"
     tRNA            6887..6971
                     /locus_tag="ACHW4Z_001253"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHW4Z_001254"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHW4Z_001254"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHW4Z_001255"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHW4Z_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR8880975.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHW4Z_001256"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHW4Z_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR8880976.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHW4Z_001257"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHW4Z_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR8880977.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHW4Z_001258"
     CDS             complement(10252..13995)
                     /locus_tag="ACHW4Z_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR8880978.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACHW4Z_001259"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACHW4Z_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR8880979.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="ACHW4Z_001260"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="ACHW4Z_001260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR8880980.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="ACHW4Z_001261"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="ACHW4Z_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR8880981.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACHW4Z_001262"
                     /gene_synonym="iatA"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACHW4Z_001262"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR8880982.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="ACHW4Z_001263"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="ACHW4Z_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR8880983.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACHW4Z_001264"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACHW4Z_001264"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR8880984.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACHW4Z_001265"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACHW4Z_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR8880985.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="ACHW4Z_001266"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="ACHW4Z_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR8880986.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="ACHW4Z_001267"
     CDS             23392..23499
                     /locus_tag="ACHW4Z_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8880987.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACHW4Z_001268"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACHW4Z_001268"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR8880988.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACHW4Z_001269"
     CDS             complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACHW4Z_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR8880989.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACHW4Z_001270"
     CDS             complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACHW4Z_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR8880990.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACHW4Z_001271"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACHW4Z_001271"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8880991.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACHW4Z_001272"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACHW4Z_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR8880992.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACHW4Z_001273"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACHW4Z_001273"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR8880993.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="ACHW4Z_001274"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="ACHW4Z_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR8880994.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="ACHW4Z_001275"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="ACHW4Z_001275"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR8880995.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="ACHW4Z_001276"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="ACHW4Z_001276"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR8880996.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="ACHW4Z_001277"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="ACHW4Z_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR8880997.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACHW4Z_001278"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACHW4Z_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR8880998.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="ACHW4Z_001279"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="ACHW4Z_001279"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR8880999.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="ACHW4Z_001280"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="ACHW4Z_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR8881000.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="ACHW4Z_001281"
     CDS             36790..39657
                     /locus_tag="ACHW4Z_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8881001.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="ACHW4Z_001282"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="ACHW4Z_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR8881002.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="ACHW4Z_001283"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="ACHW4Z_001283"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR8881003.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="ACHW4Z_001284"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="ACHW4Z_001284"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR8881004.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="ACHW4Z_001285"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="ACHW4Z_001285"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR8881005.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="ACHW4Z_001286"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="ACHW4Z_001286"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8881006.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /gene="ycgY"
                     /locus_tag="ACHW4Z_001287"
                     /pseudo
     CDS             complement(47142..47581)
                     /gene="ycgY"
                     /locus_tag="ACHW4Z_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
BASE COUNT        12041 a        12343 c        11743 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAZSNG010000022        47664 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZSNG010000022 DAZSNG010000000
VERSION     DAZSNG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47664
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.022"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHW4Z_001288"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHW4Z_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR8881007.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHW4Z_001289"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHW4Z_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR8881008.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHW4Z_001290"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHW4Z_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR8881009.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHW4Z_001291"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHW4Z_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHW4Z_001292"
     CDS             1631..2341
                     /locus_tag="ACHW4Z_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR8881010.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHW4Z_001293"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHW4Z_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR8881011.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHW4Z_001294"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHW4Z_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHW4Z_001295"
     CDS             4282..6480
                     /locus_tag="ACHW4Z_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR8881012.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHW4Z_001296"
     CDS             complement(6449..6889)
                     /locus_tag="ACHW4Z_001296"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8881013.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHW4Z_001297"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHW4Z_001297"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR8881014.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHW4Z_001298"
     CDS             7607..8305
                     /locus_tag="ACHW4Z_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR8881015.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHW4Z_001299"
     CDS             8534..9742
                     /locus_tag="ACHW4Z_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881016.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHW4Z_001300"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHW4Z_001300"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR8881017.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHW4Z_001301"
     tRNA            11849..11925
                     /locus_tag="ACHW4Z_001301"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHW4Z_001302"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHW4Z_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR8881018.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHW4Z_001303"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHW4Z_001303"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR8881019.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHW4Z_001304"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHW4Z_001304"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR8881020.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHW4Z_001305"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHW4Z_001305"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR8881021.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHW4Z_001306"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHW4Z_001306"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR8881022.1"
                     /translation="MSTQETTSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHW4Z_001307"
     CDS             complement(18540..19811)
                     /locus_tag="ACHW4Z_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR8881023.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="ACHW4Z_001308"
     CDS             20287..20394
                     /locus_tag="ACHW4Z_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8881024.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20770..20877
                     /gene="ldrD"
                     /locus_tag="ACHW4Z_001309"
     CDS             20770..20877
                     /gene="ldrD"
                     /locus_tag="ACHW4Z_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR8881025.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21253..21360
                     /locus_tag="ACHW4Z_001310"
     CDS             21253..21360
                     /locus_tag="ACHW4Z_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8881026.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21736..21843
                     /locus_tag="ACHW4Z_001311"
     CDS             21736..21843
                     /locus_tag="ACHW4Z_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8881027.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACHW4Z_001312"
     CDS             complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACHW4Z_001312"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR8881028.1"
                     /translation="MTQFTQNTAMPSSLWQYWSGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACHW4Z_001313"
     CDS             complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACHW4Z_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR8881029.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACHW4Z_001314"
     CDS             complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACHW4Z_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR8881030.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25638..25826
                     /gene="bcsR"
                     /locus_tag="ACHW4Z_001315"
     CDS             25638..25826
                     /gene="bcsR"
                     /locus_tag="ACHW4Z_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR8881031.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACHW4Z_001316"
     CDS             25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACHW4Z_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR8881032.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26587..29204
                     /gene="bcsA"
                     /locus_tag="ACHW4Z_001317"
                     /pseudo
     CDS             26587..29204
                     /gene="bcsA"
                     /locus_tag="ACHW4Z_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29215..31554
                     /gene="bcsB"
                     /locus_tag="ACHW4Z_001318"
     CDS             29215..31554
                     /gene="bcsB"
                     /locus_tag="ACHW4Z_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR8881033.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACHW4Z_001319"
     CDS             31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACHW4Z_001319"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR8881034.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32862..33251
                     /locus_tag="ACHW4Z_001320"
     CDS             32862..33251
                     /locus_tag="ACHW4Z_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR8881035.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            33333..35321
                     /gene="hmsP"
                     /locus_tag="ACHW4Z_001321"
     CDS             33333..35321
                     /gene="hmsP"
                     /locus_tag="ACHW4Z_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR8881036.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            35510..36796
                     /gene="dctA"
                     /locus_tag="ACHW4Z_001322"
     CDS             35510..36796
                     /gene="dctA"
                     /locus_tag="ACHW4Z_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR8881037.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            37017..38513
                     /locus_tag="ACHW4Z_001323"
     CDS             37017..38513
                     /locus_tag="ACHW4Z_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8881038.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(38609..39538)
                     /gene="kdgK"
                     /locus_tag="ACHW4Z_001324"
     CDS             complement(38609..39538)
                     /gene="kdgK"
                     /locus_tag="ACHW4Z_001324"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR8881039.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            39770..40537
                     /gene="pdeH"
                     /locus_tag="ACHW4Z_001325"
     CDS             39770..40537
                     /gene="pdeH"
                     /locus_tag="ACHW4Z_001325"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR8881040.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            40607..42667
                     /locus_tag="ACHW4Z_001326"
     CDS             40607..42667
                     /locus_tag="ACHW4Z_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8881041.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(42849..44171)
                     /locus_tag="ACHW4Z_001327"
     CDS             complement(42849..44171)
                     /locus_tag="ACHW4Z_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881042.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(44582..45595)
                     /gene="yhjD"
                     /locus_tag="ACHW4Z_001328"
     CDS             complement(44582..45595)
                     /gene="yhjD"
                     /locus_tag="ACHW4Z_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR8881043.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(45644..46543)
                     /locus_tag="ACHW4Z_001329"
     CDS             complement(45644..46543)
                     /locus_tag="ACHW4Z_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8881044.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            47063..>47664
                     /locus_tag="ACHW4Z_001330"
     CDS             47063..>47664
                     /locus_tag="ACHW4Z_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR8881045.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11414 a        11877 c        12826 g        11547 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatagct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 accacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggggtgaaa
    20461 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    20521 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    20581 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    20641 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    20701 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
    20761 ggcggctata tgacgttcgc agaactgggc atggccttct ggcatgattt agcggctccg
    20821 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    20881 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg
    20941 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
    21001 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
    21061 cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    21121 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    21181 cgggggccat tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac
    21241 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    21301 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    21361 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
    21421 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
    21481 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
    21541 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    21601 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    21661 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
    21721 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
    21781 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    21841 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    21901 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    21961 ttcaggcgaa cgtacggttt atcttggtat tgaataacta ctgcatttga gttctcggag
    22021 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    22081 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt
    22141 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    22201 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    22261 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    22321 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    22381 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    22441 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
    22501 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    22561 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    22621 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    22681 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    22741 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    22801 ttcttgaaat caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    22861 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    22921 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    22981 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    23041 accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
    23101 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    23161 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    23221 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    23281 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    23341 ctttccgggc caggcaacca gatgtcatgc cagaacaaag caaagccgat cggcagggca
    23401 atccagtggc gcaagcgatg caggctgtaa cgcggaatgg gcatcagcag aaacgcggca
    23461 aacaccaaat tgaggagcgg gtggaagtta agatatcccg cccacaacag gccaaactta
    23521 accagaaaat agaagttcca gccggaaagg ccgctccagt attgccagag ggaagaaggc
    23581 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
    23641 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc
    23701 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    23761 cgataataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    23821 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    23881 gaccggttta gaactttgcg ttaaaggcag cggcattccc cattgttctg gggcaagcag
    23941 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    24001 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    24061 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    24121 acggttaggg cgacatagcg tcagggcttg ctcaacgcgg atacccggta ccggacgtag
    24181 ggcaaccaga acgcctttac cgtgggcagg caagagaggg ttattcatca tgttgttgac
    24241 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    24301 cagcaaggta gtgatatctt ccggtacata gcgactaaac ttctgccctt gcacgctttc
    24361 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    24421 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    24481 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    24541 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    24601 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    24661 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    24721 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    24781 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    24841 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    24901 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    24961 attcatttta tccaaccatg agcgaagccg ctcggcagga atattttgcc atgcgttatt
    25021 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
    25081 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    25141 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    25201 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    25261 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    25321 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    25381 gattatcagt ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc
    25441 agatttagca tgtgaatcaa tttagaaggt cacaagacgt gtgattttag tcttttcgtt
    25501 tttaactttt tctgttgcgt aacggaaagt caaaaagtga gcaaattccc gtctcgccgt
    25561 aaacaataaa ccggcgaaat gctcacggtt agaattgtct catgaatggt acggttattt
    25621 catagggatc aagcaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag
    25681 gctatatctt ccagaatgat attgtggcgt taaagcaggc attttcactg cctgatattg
    25741 attatgccga tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc
    25801 tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg gccgtactgg gattgcaggg
    25861 ggtgcgggga ggcgtgggga caacaaccat caccgccgca ttagcctggt cattacaaat
    25921 gttgggagaa aatgtcctgg tggtcgatgc ctgcccggac aacttgttgc gcctgtcatt
    25981 taatgttgat tttacccacc gtcagggctg ggccagagcg atgctggatg gccaggactg
    26041 gcgtgacgct gggttgcgct acacctcgca gctcgatttg ctgccttttg gtcagttatc
    26101 cattgaagaa caagaaaatc cacagcactg gcaaacccgg ctgagcgata tttgctccgg
    26161 cttacagcaa ctaaaagcca gcgggcgtta ccagtggatt ttaatcgact taccgcgtga
    26221 tgcctcgcag ataacccacc agctgctgag tttgtgcgag cactcgctgg caatcgtcaa
    26281 tgtggatgcc aactgccata tccgactgca tcagcaagcg ctgccggatg gcgcacatat
    26341 tttgattaat gacttccgta ttggcagtca ggttcaggac gatatttacc agctttggtt
    26401 gcaaagccag cgccgattac tgccgatgct cattcatcgt gatgaagcga tggctgaatg
    26461 cctggcggct aagcaaccag taggtgaata tcgcagtgat gcgctggcgg ctgaagagat
    26521 actgacgctg gcgaactggt gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag
    26581 tgcatcatga gtatcctgac ccggtggttg cttatcccgc cggtcaacgc gcggcttatc
    26641 gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt
    26701 ttctggatga tcctggcctg gatttttatt ccgctggagc acccgcgctg gcagcgtatt
    26761 cgcgcagaac ataaaaacct gtatacgcat atcaacgcct cgcgtccgcg tccgctggac
    26821 ccggtccgtt atctcattca aacatgctgg ttattgatcg gtgcatcgcg caaagaaacg
    26881 ccgaaaccgc gcagcgggca ttttcaggtc tgcaaaatat tcgtggacgt taccatcaat
    26941 ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatctggat gaaaaaaaag
    27001 agctcggtca tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc gtcaccttct
    27061 cgctgattct ggcgttaatc tgcgttactc agccgtttaa cccgctggcg cagtttatct
    27121 tcctgatgct gctgtggggg gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg
    27181 cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg ttatatctgg tggcgttaca
    27241 cctctacgct gaactgggac gatccggtca gcctggtgtg cgggcttatt ctgctcttcg
    27301 ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt ccaggtagta tggccgctga
    27361 atcgtcagcc ggtgccattg ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg
    27421 tcccgactta caacgaagat ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta
    27481 tcgactggcc gaaagacaaa ctgaacatct ggatcctcga tgacggcggc agggaagagt
    27541 ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgcaccact catgaacatg
    27601 cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc gtgtcgattt
    27661 tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc tggttcctga
    27721 aagagaaaca gctggcgatg atgcagactc cacaccactt cttctcgccg gacccgtttg
    27781 aacgcaacct gggacgtttt cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg
    27841 tgcaggatgg caacgatatg tgggacgcca ctttcttctg cggttcctgt gcggtgattc
    27901 gtcgtaagcc gctggatgaa attggcggca ttgctgtcga aaccgtgact gaagatgcgc
    27961 atacttctct gcgtttgcac cgtcgtggct atacctccgc gtatatgcgt attccgcagg
    28021 cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc
    28081 gcgggatggt ataaatcttc cgtctcgata acccgctcac cggtaaagga ctgaagtttg
    28141 ctcagcggct gtgctacgtc aacgccatgt tccacttctt gtcgggcatt ccacggctga
    28201 tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag
    28261 cgttgatgat cgccctattc gtgctgccgc atatgatcca tgccagcctg accaactcga
    28321 agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg gtgctggcgt
    28381 ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg
    28441 tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc tcgcggccct
    28501 acatcttcct tgttctactc aacctggtgg gcgttgcggt aggcatctgg cgctacttct
    28561 atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc
    28621 tgattgttct tggcggcgca gttgcggtat cggtagaaag caaacaggta cgccgatcga
    28681 accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt
    28741 gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt caggcgcaga
    28801 ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc
    28861 cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg ccgctcacca
    28921 cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca tgggcgctct
    28981 ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt ctgaagctcg
    29041 gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc
    29101 gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc ccggagcgga
    29161 gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa cgcgatgaaa
    29221 agaaaactat tctggatttg tgcagtggct atggggatga gtgcgttccc ctctttcatg
    29281 acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt agccgcccag
    29341 acggaacaaa atccgcaggt ggggcaagtg atgtcaggcg agcagggagc tgatgccccc
    29401 atcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca aatcgcgccg
    29461 ccgccgggca gcatggtgct acgtggtatt aacccgaacg gcagcattga gtttggtatg
    29521 cgcagcgatg aagtggtgac gaaggcgatg ctcaacttcg aatacacgcc atcgccatcg
    29581 ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgagctgat gggcgtgctg
    29641 ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat taatccactg
    29701 tttattaccg acttcaaccg tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc
    29761 gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg actggatctg
    29821 acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc attctttgac
    29881 ccgcgcgata accgtactaa caccttgccg atggtctttg cgggtgcgcc ggatgttggg
    29941 ctgcaacaag catctgccat tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg
    30001 cagaacttcc cggtgctcta taaccaattg ccggatcgca atgccattgt ctttgcaacc
    30061 aacgacaaac ggccggactt cctgcgcgat catccggcgg taaaagcccc ggtgattgag
    30121 atgattaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac
    30181 aaagacctgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt ccgtggtgaa
    30241 agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac
    30301 tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga aaacctatga agaacagtta
    30361 caatccagcg gccttgagcc agcagcgatt aacgtttcgc taaacctgcc gccggatctc
    30421 tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac catgccgccg
    30481 gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca atccttcaac
    30541 ctgagcagca aacaggaggc gaaccgtctg ctgctgcgta ttccggtatt acaaggtttg
    30601 ctggatggca aaacagatgt ctctattccg gcgctgaaac tgggcgcgac caaccagctg
    30661 cgcttcgact ttgagtatat gaacccgatg ccaggcggtt cggtagataa ctgtattacc
    30721 ttccagccgg tgcagaatca tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag
    30781 tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg cttcccattc
    30841 agccggatgg cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc taacgaagca
    30901 cagatggaaa cgttgctgaa tactgttggt tttatcggcg cacagacggg cttcccggcg
    30961 attaatctga cggtgaccga tgatggcagc accattcagg gcaaagatgc cgacatcatg
    31021 atcatcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct attggtgcag
    31081 gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc cgttccccgg cattgtgccg
    31141 gacgagagcg atcgcgcggc agaaacccag tcaacgctga cctcttccgg tgcgatggcg
    31201 gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc gctgttggca
    31261 gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg caaacgcgcc
    31321 accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct acgtgttggc
    31381 gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac
    31441 catccgattc tgctggcggt gctggcgact atcagtgtga tattgctggc atgggtactg
    31501 tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc cggataacga gtaattgaag
    31561 atgaatgtgt tgcgtagtgg aatcgtgacg atgctgttgt tggctgcctt tagtgttcag
    31621 gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag tcaggaaggg
    31681 cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca aagttacggt
    31741 atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatattct cgactggacg
    31801 cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag
    31861 aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg tgatgtctgg
    31921 atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta taccgacatc
    31981 ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt
    32041 tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg ttttaacccc
    32101 agctacatgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg
    32161 ctgcgcgaaa ccaatcaaca tttattgctg gaaaccgccc cgaaaggctt ttcgccagac
    32221 tgggtgcgct atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac attgatcagc
    32281 agctacgacg ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa
    32341 aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa aaacggttat
    32401 ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt
    32461 tctgccgcca tgctgccttt tttacaaaac cgcgatgcgc aggcagttca gcgccagcgc
    32521 gtggccgata actttcccgg cagcgatacc tattacaact atgtgctgac cctgtttgga
    32581 caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt acctgactgg
    32641 ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt ctggccgtca
    32701 tgccgatggt cgaggcagca ccaaccatca gcagaccctg cggatggaca gcggcaacac
    32761 taatgccgtg cgcgggctgg cgaatattta ccgccagcag tcgccagaaa aagctgaagc
    32821 gtttatcgcc tcgctctctg ccagccagcg gcgcagcatt gatgatatcg aacgcagcct
    32881 gcaaaacgac cgtctggcac agcaggcaga ggtactggaa aaccagggca aatgggcgca
    32941 ggcggcagca cttcagcggc aacgactggc gctgggcccc ggcagcgtat ggattactta
    33001 ccgactggca ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg
    33061 gctacacggc gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg
    33121 caattgatat ccagcaggtg aaagattacg cacccagcca tttcctgctc tacgtacgtt
    33181 attccgccgc cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg
    33241 ccgactggta agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg
    33301 gcggcaatct gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca
    33361 agcagatggc aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt
    33421 tgctgttcca tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg
    33481 ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg
    33541 aagctattct tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc
    33601 ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga gcgtccggtg ccggtaatgg
    33661 ttactcgcct gtttgagcta ccggttcaaa tctcgctggg cgtctactcg ctcgaacgtc
    33721 cggcaaatcc gcagccaata gcctatctgg tgctacaggc ggattccttc cgtatgtata
    33781 agttcgtgat gagcactctc tcaacgttag tgaccattta cttacttttg tcgcttatcc
    33841 tgaccgtcgc catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg
    33901 ctcgcgaact taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc
    33961 gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat
    34021 tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt
    34081 tgccgaacaa agccttgctg atggagatgc tggagcaggt tgtcgcgcgt aaacaaacca
    34141 ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg
    34201 cgcaacgaga aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac
    34261 gtatgatcct cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg
    34321 aaccgtggca cgcaatcact ttgggtcagc aagtactcac tatcatgagc gagcgcctgc
    34381 cgattgaacg tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg
    34441 atctcaccgc cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata
    34501 aaggcaagaa tcagattcag ttctttgatc cgcagcagat ggaagcagcc cagaagcggt
    34561 tgacggaaga gagcgatatc cttaatgcac tggaaaatca tcagtttgca atttggttac
    34621 agccacaggt cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta ctgcgtatcc
    34681 agcaaccgga tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg
    34741 ggctgatggt taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct
    34801 ggcaagagcg cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc
    34861 acccgaatat ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa
    34921 cactgattct ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa
    34981 tcctccgtcc gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct
    35041 acgcagggct gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctgaaaatcg
    35101 acaaaatgtt tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc
    35161 tggcgcagag cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgag
    35221 actggctggc aaaagcgggc gttggtattg cccagggctt cctttttgct cgcccactcc
    35281 ctattgaaat cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca
    35341 aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca
    35401 attatgatcc aggtacattt ctgtgatgtt gtctgggtgt tattttaagg ccgcaggtac
    35461 ccccccccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    35521 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    35581 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    35641 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    35701 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    35761 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    35821 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    35881 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    35941 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    36001 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt tctcgcaggt
    36061 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgc tcggggcaat
    36121 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    36181 ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    36241 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    36301 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    36361 ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    36421 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    36481 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    36541 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    36601 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    36661 gctaactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    36721 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    36781 cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt
    36841 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    36901 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    36961 gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc
    37021 agggcataaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    37081 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    37141 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    37201 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    37261 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    37321 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    37381 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    37441 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    37501 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    37561 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    37621 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    37681 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    37741 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    37801 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    37861 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    37921 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtgacagc aaagacatcg
    37981 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    38041 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    38101 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    38161 tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg ggccagcgga
    38221 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    38281 aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt cagcagttgt
    38341 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    38401 cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg accacctctg
    38461 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    38521 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    38581 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    38641 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    38701 cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    38761 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatcaact aacgcttcac
    38821 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    38881 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcgt
    38941 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgctggtac acctgctgtg
    39001 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    39061 ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    39121 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    39181 ccgcagactg ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc cagtagtaga
    39241 acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga cggttttcca
    39301 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atctgctggc
    39361 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    39421 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    39481 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    39541 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    39601 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    39661 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgccctta cgcgccgata
    39721 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    39781 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    39841 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcgggcggtt
    39901 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    39961 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    40021 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacggcctgc tggcatcggt
    40081 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    40141 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    40201 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat
    40261 ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact
    40321 gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    40381 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    40441 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    40501 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgccgggct
    40561 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    40621 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    40681 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    40741 gttgaatcgt ccgttcgcta tccgtggcaa tctgggcgtg gtgtgggagc ggcaaaaaca
    40801 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    40861 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    40921 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    40981 gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatcttgc
    41041 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    41101 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    41161 aatttttgtt gatccgttag gcaagccgct gccattcagc gaagttactg gatcgaaagg
    41221 taaagcggat aaagaaaagg tgggcgatta catttttggc ctgaaggcgc agggacgtta
    41281 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    41341 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    41401 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    41461 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    41521 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    41581 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    41641 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    41701 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    41761 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    41821 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    41881 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    41941 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca
    42001 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    42061 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    42121 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    42181 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    42241 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    42301 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    42361 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cggaacagct
    42421 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    42481 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    42541 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc
    42601 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    42661 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    42721 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    42781 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    42841 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    42901 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agcctgcaac
    42961 caggctgcga tatacggcgc aacggaagcc ccgagaatcg acgatacgtt gtaagagaac
    43021 gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccatcggc
    43081 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    43141 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    43201 atgatcacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    43261 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    43321 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    43381 aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg
    43441 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    43501 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag
    43561 aagataaatg gcacacgcca gccccagctc ataaactgct catcggtcag tagccaggaa
    43621 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag
    43681 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    43741 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagtggg
    43801 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt cgaaatcccc
    43861 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    43921 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    43981 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    44041 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    44101 aggactttat tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct
    44161 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    44221 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    44281 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    44341 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    44401 agttggagtg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    44461 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    44521 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    44581 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    44641 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    44701 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    44761 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    44821 tgcgcggacg gtggcgcggc aaccgccaga agatccagaa gaaaagcaga tagttggcga
    44881 agatggaaat cgccaggcca atcaatcgcc acgtcggttt cagccactca atgctgttca
    44941 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    45001 tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga tattttaccc
    45061 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcacgaatcg
    45121 cttcacgcag gttacccatc cagttgatgc cggaataaag tgccaccgcc aggccgacaa
    45181 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    45241 gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    45301 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    45361 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    45421 ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    45481 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    45541 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    45601 cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc aggtgctctt
    45661 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    45721 gcgggtaaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    45781 gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    45841 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    45901 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    45961 gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    46021 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt tagtttgccg aggggacggg
    46081 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    46141 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    46201 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    46261 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    46321 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    46381 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    46441 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    46501 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    46561 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    46621 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    46681 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    46741 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    46801 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    46861 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    46921 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    46981 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    47041 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    47101 tcatggaata aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggtcag
    47161 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    47221 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    47281 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    47341 ccattttaat gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat ttgcgttggc
    47401 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    47461 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    47521 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    47581 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    47641 aatgatgtta aatatctcct cctg
//
LOCUS       DAZSNG010000023        47463 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZSNG010000023 DAZSNG010000000
VERSION     DAZSNG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.023"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="ACHW4Z_001331"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="ACHW4Z_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            1316..1441
                     /gene="timP"
                     /locus_tag="ACHW4Z_001332"
     CDS             1316..1441
                     /gene="timP"
                     /locus_tag="ACHW4Z_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR8881046.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACHW4Z_001333"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACHW4Z_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR8881047.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACHW4Z_001334"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACHW4Z_001334"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR8881048.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACHW4Z_001335"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACHW4Z_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR8881049.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACHW4Z_001336"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACHW4Z_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR8881050.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACHW4Z_001337"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACHW4Z_001337"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR8881051.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACHW4Z_001338"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACHW4Z_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR8881052.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACHW4Z_001339"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACHW4Z_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR8881053.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACHW4Z_001340"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACHW4Z_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR8881054.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACHW4Z_001341"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACHW4Z_001341"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR8881055.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACHW4Z_001342"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACHW4Z_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR8881056.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACHW4Z_001343"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACHW4Z_001343"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR8881057.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="ACHW4Z_001344"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="ACHW4Z_001344"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR8881058.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="ACHW4Z_001345"
     CDS             11405..12259
                     /locus_tag="ACHW4Z_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8881059.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="ACHW4Z_001346"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="ACHW4Z_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR8881060.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="ACHW4Z_001347"
     CDS             complement(13722..14861)
                     /locus_tag="ACHW4Z_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR8881061.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACHW4Z_001348"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACHW4Z_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR8881062.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /gene="hcaE"
                     /locus_tag="ACHW4Z_001349"
     CDS             16047..17408
                     /gene="hcaE"
                     /locus_tag="ACHW4Z_001349"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR8881063.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /gene="hcaF"
                     /locus_tag="ACHW4Z_001350"
     CDS             17405..17923
                     /gene="hcaF"
                     /locus_tag="ACHW4Z_001350"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR8881064.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="ACHW4Z_001351"
     CDS             17923..18243
                     /locus_tag="ACHW4Z_001351"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR8881065.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="ACHW4Z_001352"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="ACHW4Z_001352"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR8881066.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20279
                     /gene="hcaD"
                     /locus_tag="ACHW4Z_001353"
     CDS             19062..20279
                     /gene="hcaD"
                     /locus_tag="ACHW4Z_001353"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR8881067.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20376..20798
                     /locus_tag="ACHW4Z_001354"
     CDS             20376..20798
                     /locus_tag="ACHW4Z_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8881068.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20846..21718)
                     /locus_tag="ACHW4Z_001355"
     CDS             complement(20846..21718)
                     /locus_tag="ACHW4Z_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR8881069.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21730..22824)
                     /locus_tag="ACHW4Z_001356"
     CDS             complement(21730..22824)
                     /locus_tag="ACHW4Z_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8881070.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22857..23855)
                     /locus_tag="ACHW4Z_001357"
     CDS             complement(22857..23855)
                     /locus_tag="ACHW4Z_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8881071.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23880..25391)
                     /locus_tag="ACHW4Z_001358"
     CDS             complement(23880..25391)
                     /locus_tag="ACHW4Z_001358"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR8881072.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25414..26396)
                     /locus_tag="ACHW4Z_001359"
                     /pseudo
     CDS             complement(25414..26396)
                     /locus_tag="ACHW4Z_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26493..29774)
                     /locus_tag="ACHW4Z_001360"
                     /pseudo
     CDS             complement(26493..29774)
                     /locus_tag="ACHW4Z_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29892..31085
                     /locus_tag="ACHW4Z_001361"
     CDS             29892..31085
                     /locus_tag="ACHW4Z_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR8881073.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="ACHW4Z_001362"
     CDS             complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="ACHW4Z_001362"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR8881074.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32863..34053
                     /gene="hmpA"
                     /locus_tag="ACHW4Z_001363"
     CDS             32863..34053
                     /gene="hmpA"
                     /locus_tag="ACHW4Z_001363"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR8881075.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="ACHW4Z_001364"
     CDS             complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="ACHW4Z_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR8881076.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="ACHW4Z_001365"
     CDS             complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="ACHW4Z_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR8881077.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="ACHW4Z_001366"
     CDS             complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="ACHW4Z_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR8881078.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIECQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="ACHW4Z_001367"
     CDS             complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="ACHW4Z_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR8881079.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38684..42571)
                     /gene="purL"
                     /locus_tag="ACHW4Z_001368"
                     /gene_synonym="purI"
     CDS             complement(38684..42571)
                     /gene="purL"
                     /locus_tag="ACHW4Z_001368"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR8881080.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42830..44386
                     /gene="mltF"
                     /locus_tag="ACHW4Z_001369"
     CDS             42830..44386
                     /gene="mltF"
                     /locus_tag="ACHW4Z_001369"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR8881081.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44383..44886)
                     /gene="tadA"
                     /locus_tag="ACHW4Z_001370"
     CDS             complement(44383..44886)
                     /gene="tadA"
                     /locus_tag="ACHW4Z_001370"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR8881082.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="ACHW4Z_001371"
     CDS             complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="ACHW4Z_001371"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR8881083.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45788..46636
                     /locus_tag="ACHW4Z_001372"
     CDS             45788..46636
                     /locus_tag="ACHW4Z_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR8881084.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46975..47463)
                     /locus_tag="ACHW4Z_001373"
                     /pseudo
     CDS             complement(46975..47463)
                     /locus_tag="ACHW4Z_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11316 a        13012 c        11942 g        11193 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc
    19621 gtaatgcacc accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc
    19681 gcattctgct caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc
    19741 tgcaaagtgg ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca
    19801 acgagcaact ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg
    19861 cttgccgcac ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg
    19921 ataatggtgc actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg
    19981 ccgctgccgc aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg
    20041 atcagtacag tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc
    20101 gtggcaaccc ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg
    20161 gtgcggtcac gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg
    20221 gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat
    20281 caggataatt agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta
    20341 cgtttaaatc acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt
    20401 ttggagctgc ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca
    20461 ttatttttgg ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt
    20521 tgggcgcgcc aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg
    20581 cgatattaat cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca
    20641 cgctgggtac ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc
    20701 caaatatgat taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa
    20761 ttaccggacc gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg
    20821 aacgccttat ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg
    20881 ttgttgattc ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat
    20941 ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg
    21001 cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg
    21061 cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt
    21121 tcacccactg acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct
    21181 cgcaaaactc acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct
    21241 gaatccgcgt ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg
    21301 tctctgcccc ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa
    21361 acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac
    21421 tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt
    21481 gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt
    21541 tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac
    21601 cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc
    21661 cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc
    21721 gctaactcct taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag
    21781 cacataggca tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca
    21841 cagcttccag tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca
    21901 ggagccgata atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt
    21961 tccggtttca ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa
    22021 gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc
    22081 agcaattatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa
    22141 ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc
    22201 gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac
    22261 gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca
    22321 actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc
    22381 ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc
    22441 tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga
    22501 aatgtgatac accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc
    22561 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
    22621 tgccgccgcc gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc
    22681 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
    22741 cagatcgacg gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc
    22801 attaacaata cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag
    22861 cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
    22921 atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta
    22981 ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg
    23041 gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
    23101 gcacctgcgt taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat
    23161 aatcccgaaa gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc
    23221 gccgccgtgg cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta
    23281 atgaacacaa acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga
    23341 aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg
    23401 gtcataaaca gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt
    23461 ggtacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc
    23521 aacaccagca gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac
    23581 accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
    23641 agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg
    23701 ttattcaatg agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc
    23761 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
    23821 ttgccctgcg gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat
    23881 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc
    23941 tgcgagaacg tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc
    24001 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg
    24061 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
    24121 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc
    24181 gtgccgatgg gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg
    24241 gtcaggcggc ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg
    24301 gtcagcactg tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt
    24361 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc
    24421 tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc
    24481 aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc
    24541 gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc
    24601 tgatccacaa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt
    24661 agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca
    24721 tcgcgcataa cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca
    24781 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact
    24841 tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
    24901 atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt
    24961 tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc
    25021 aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc
    25081 gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca
    25141 cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
    25201 ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt
    25261 ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
    25321 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
    25381 gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
    25441 cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt
    25501 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat
    25561 tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc
    25621 aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg
    25681 taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc
    25741 cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc
    25801 ctggttagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt
    25861 acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
    25921 aattttattg gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt
    25981 ggatcgccga ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg
    26041 ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca
    26101 ttttcagaca ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca
    26161 aaggtacttt ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact
    26221 gaagaatctt ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta
    26281 tcaaggtata ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta
    26341 cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg
    26401 cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc
    26461 gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc
    26521 atcacagtgg tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg
    26581 cgagcagact catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc
    26641 aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt
    26701 gaaacagcaa agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc
    26761 atttcactaa acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct
    26821 tgccagaaga gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga
    26881 tcgccggtcg ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg
    26941 gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg
    27001 cttaaattct ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct
    27061 ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc
    27121 ttcccttccc cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg
    27181 tctgcctgac ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc
    27241 agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt
    27301 aacttatcca gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa
    27361 gcattatcaa gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc
    27421 gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag
    27481 gcaatggctt tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca
    27541 aaccaacact cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg
    27601 acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc
    27661 agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc
    27721 tctgccgcca gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta
    27781 acgtcgcgtc cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg
    27841 aaccagttca gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc
    27901 tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc
    27961 acttcctgat tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg
    28021 aagttatcat tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg
    28081 ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg
    28141 taagcactgg cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt
    28201 gcacgtttca gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat
    28261 tccagcatcg ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc
    28321 aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata
    28381 aaccaggctt catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc
    28441 ggcaacggca actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca
    28501 tcggagagtt caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg
    28561 gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg
    28621 atcgccagac ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc
    28681 tcactacgct ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa
    28741 tgataaggca ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa
    28801 tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg
    28861 gtcagactct tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta
    28921 cctggcgcaa tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac
    28981 gcgccgacaa aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt
    29041 ttatagcgag aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg
    29101 atggggaaag cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg
    29161 aaaacgctct gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga
    29221 cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg
    29281 cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac
    29341 accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc
    29401 gggcgatggt gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc
    29461 cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta
    29521 tcccatgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg
    29581 ttttccagcc acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc
    29641 acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg
    29701 ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact
    29761 tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg
    29821 cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag
    29881 gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg
    29941 tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg
    30001 cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg
    30061 tggtgaatat tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg
    30121 cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg
    30181 ttgctaatgc ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga
    30241 cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc
    30301 cggaccgcac catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg
    30361 tatcgcaaac catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg
    30421 aagagaagct gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg
    30481 agaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca
    30541 ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac
    30601 agattggtca caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca
    30661 gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat
    30721 cacaaccggt taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg
    30781 cctggcagag tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg
    30841 gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc
    30901 gcagttgtgc ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc
    30961 cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa
    31021 gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga
    31081 tttgatggcg cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa
    31141 aaattgcctg atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg
    31201 caagattttg taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat
    31261 tgtcagcaaa tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
    31321 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
    31381 tccagccagc cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag
    31441 tacctacacg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
    31501 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
    31561 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
    31621 cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag
    31681 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
    31741 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
    31801 cgctaccacc tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
    31861 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
    31921 ccgcaacgtg cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca
    31981 ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct
    32041 tgtgttcttt ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac
    32101 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
    32161 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
    32221 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
    32281 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
    32341 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
    32401 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
    32461 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
    32521 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
    32581 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
    32641 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
    32701 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
    32761 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
    32821 catttgagat acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc
    32881 atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc
    32941 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
    33001 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
    33061 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
    33121 agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg
    33181 gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta
    33241 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
    33301 ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt
    33361 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
    33421 caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac
    33481 tctttgactc gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt
    33541 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
    33601 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
    33661 accggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
    33721 acagcacaag tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat
    33781 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
    33841 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
    33901 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
    33961 ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
    34021 tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg
    34081 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
    34141 acccgagcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
    34201 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
    34261 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
    34321 tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt
    34381 gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta
    34441 ttccttgaaa aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
    34501 tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg
    34561 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta
    34621 cgcaagtagt tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt
    34681 tcaccctcca gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc
    34741 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc
    34801 aggcgtttca tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc
    34861 tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga
    34921 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
    34981 atcgcttttg acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
    35041 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt
    35101 aacggcgcag gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg
    35161 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
    35221 tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg
    35281 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga
    35341 ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
    35401 agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg
    35461 gattgctcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg
    35521 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca
    35581 gtaagaataa ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata
    35641 ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt
    35701 acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg
    35761 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
    35821 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg
    35881 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcatt caatatcggt
    35941 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
    36001 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc
    36061 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
    36121 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
    36181 cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
    36241 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
    36301 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat
    36361 gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
    36421 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg
    36481 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
    36541 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggactgcg
    36601 cggagaaacc ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac
    36661 tgacaaataa aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac
    36721 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
    36781 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc
    36841 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
    36901 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
    36961 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
    37021 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
    37081 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc
    37141 ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca
    37201 agcagttgtt cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc
    37261 tctttttgct ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg
    37321 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
    37381 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
    37441 aactcgctcg gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg
    37501 ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc
    37561 atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
    37621 gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc
    37681 atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca
    37741 ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt
    37801 ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
    37861 ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc
    37921 tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg
    37981 cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc
    38041 agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag
    38101 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
    38161 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
    38221 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
    38281 gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg
    38341 gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa
    38401 gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc
    38461 tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt
    38521 agattgcaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat
    38581 tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt
    38641 gcttaatgcc gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac
    38701 gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg
    38761 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac
    38821 tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt
    38881 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
    38941 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
    39001 gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca
    39061 ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac
    39121 tccccgggat cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg
    39181 ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
    39241 ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc
    39301 acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat
    39361 gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt
    39421 tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg
    39481 ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt
    39541 tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
    39601 gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct
    39661 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc
    39721 aatccgcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
    39781 cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
    39841 aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca
    39901 gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct
    39961 gaatcgcgtc atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc
    40021 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
    40081 aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta
    40141 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
    40201 cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg
    40261 tcaggcccag cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg
    40321 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga
    40381 tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag
    40441 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
    40501 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
    40561 ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga
    40621 aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga
    40681 tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct
    40741 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
    40801 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
    40861 gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
    40921 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
    40981 gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg
    41041 cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa
    41101 gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct
    41161 gtacggaagc aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc
    41221 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt
    41281 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    41341 tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag
    41401 tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca
    41461 gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt
    41521 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac
    41581 cagctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    41641 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    41701 tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt
    41761 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    41821 gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt
    41881 caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct
    41941 gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    42001 gcagatagtc aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca
    42061 gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg
    42121 caaacaactg ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca
    42181 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    42241 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg
    42301 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    42361 gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct
    42421 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    42481 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    42541 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    42601 acccaagggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    42661 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    42721 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    42781 tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt
    42841 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
    42901 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
    42961 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
    43021 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
    43081 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
    43141 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
    43201 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
    43261 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct
    43321 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
    43381 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
    43441 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
    43501 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
    43561 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
    43621 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
    43681 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
    43741 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
    43801 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
    43861 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
    43921 gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta
    43981 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
    44041 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
    44101 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
    44161 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
    44221 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
    44281 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
    44341 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
    44401 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
    44461 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
    44521 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
    44581 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
    44641 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
    44701 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
    44761 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
    44821 ggcacgtttc gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc
    44881 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
    44941 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
    45001 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
    45061 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
    45121 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
    45181 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
    45241 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
    45301 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
    45361 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
    45421 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
    45481 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
    45541 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
    45601 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
    45661 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
    45721 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
    45781 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga
    45841 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
    45901 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
    45961 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
    46021 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
    46081 cggcgaaaaa ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag
    46141 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
    46201 cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg
    46261 cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
    46321 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
    46381 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
    46441 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
    46501 cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
    46561 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
    46621 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
    46681 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa
    46741 tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt
    46801 ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt
    46861 taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa
    46921 cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat
    46981 aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac
    47041 cttctggcga gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga
    47101 aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt
    47161 tgttcgttca acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa
    47221 aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc
    47281 tcgttcgcgc aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc
    47341 cagcttccag acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc
    47401 cggtctttcg gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca
    47461 gtt
//
LOCUS       DAZSNG010000024        45786 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZSNG010000024 DAZSNG010000000
VERSION     DAZSNG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.024"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..2248
                     /locus_tag="ACHW4Z_001374"
     CDS             95..2248
                     /locus_tag="ACHW4Z_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR8881085.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="ACHW4Z_001375"
     CDS             2301..4031
                     /locus_tag="ACHW4Z_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8881086.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="ACHW4Z_001376"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="ACHW4Z_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR8881087.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="ACHW4Z_001377"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="ACHW4Z_001377"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR8881088.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="ACHW4Z_001378"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="ACHW4Z_001378"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR8881089.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="ACHW4Z_001379"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="ACHW4Z_001379"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR8881090.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="ACHW4Z_001380"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="ACHW4Z_001380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR8881091.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="ACHW4Z_001381"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="ACHW4Z_001381"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR8881092.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="ACHW4Z_001382"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="ACHW4Z_001382"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR8881093.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="ACHW4Z_001383"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="ACHW4Z_001383"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR8881094.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="ACHW4Z_001384"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="ACHW4Z_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR8881095.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="ACHW4Z_001385"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="ACHW4Z_001385"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR8881096.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="ACHW4Z_001386"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="ACHW4Z_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR8881097.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="ACHW4Z_001387"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="ACHW4Z_001387"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR8881098.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="ACHW4Z_001388"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="ACHW4Z_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR8881099.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="ACHW4Z_001389"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="ACHW4Z_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR8881100.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="ACHW4Z_001390"
     CDS             16289..16828
                     /locus_tag="ACHW4Z_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8881101.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="ACHW4Z_001391"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="ACHW4Z_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR8881102.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /gene="yajD"
                     /locus_tag="ACHW4Z_001392"
     CDS             complement(18187..18534)
                     /gene="yajD"
                     /locus_tag="ACHW4Z_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR8881103.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="ACHW4Z_001393"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="ACHW4Z_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR8881104.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="ACHW4Z_001394"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="ACHW4Z_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR8881105.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="ACHW4Z_001395"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="ACHW4Z_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR8881106.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="ACHW4Z_001396"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="ACHW4Z_001396"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR8881107.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="ACHW4Z_001397"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="ACHW4Z_001397"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR8881108.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="ACHW4Z_001398"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="ACHW4Z_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="ACHW4Z_001399"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="ACHW4Z_001399"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR8881109.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="ACHW4Z_001400"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="ACHW4Z_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR8881110.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="ACHW4Z_001401"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="ACHW4Z_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR8881111.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="ACHW4Z_001402"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="ACHW4Z_001402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR8881112.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="ACHW4Z_001403"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="ACHW4Z_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR8881113.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="ACHW4Z_001404"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="ACHW4Z_001404"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR8881114.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="ACHW4Z_001405"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="ACHW4Z_001405"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR8881115.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="ACHW4Z_001406"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="ACHW4Z_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR8881116.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="ACHW4Z_001407"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="ACHW4Z_001407"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR8881117.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="ACHW4Z_001408"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="ACHW4Z_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR8881118.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="ACHW4Z_001409"
     CDS             complement(39992..40447)
                     /locus_tag="ACHW4Z_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881119.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="ACHW4Z_001410"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="ACHW4Z_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="ACHW4Z_001411"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="ACHW4Z_001411"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR8881120.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="ACHW4Z_001412"
     CDS             complement(41290..41967)
                     /locus_tag="ACHW4Z_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR8881121.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="ACHW4Z_001413"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="ACHW4Z_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR8881122.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="ACHW4Z_001414"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="ACHW4Z_001414"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR8881123.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="ACHW4Z_001415"
     CDS             complement(43173..43631)
                     /locus_tag="ACHW4Z_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR8881124.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="ACHW4Z_001416"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="ACHW4Z_001416"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR8881125.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="ACHW4Z_001417"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="ACHW4Z_001417"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR8881126.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11901 a        11944 c        11202 g        10739 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcacta
//
LOCUS       DAZSNG010000025        42870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZSNG010000025 DAZSNG010000000
VERSION     DAZSNG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.025"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHW4Z_001418"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHW4Z_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR8881127.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHW4Z_001419"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHW4Z_001419"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR8881128.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHW4Z_001420"
     CDS             complement(3736..4092)
                     /locus_tag="ACHW4Z_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8881129.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHW4Z_001421"
     CDS             complement(4096..4512)
                     /locus_tag="ACHW4Z_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR8881130.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHW4Z_001422"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHW4Z_001422"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR8881131.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="ACHW4Z_001423"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="ACHW4Z_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="ACHW4Z_001424"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="ACHW4Z_001424"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR8881132.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="ACHW4Z_001425"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="ACHW4Z_001425"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR8881133.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="ACHW4Z_001426"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="ACHW4Z_001426"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8881134.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="ACHW4Z_001427"
     CDS             10521..11504
                     /locus_tag="ACHW4Z_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR8881135.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="ACHW4Z_001428"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="ACHW4Z_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR8881136.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="ACHW4Z_001429"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="ACHW4Z_001429"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR8881137.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14153)
                     /locus_tag="ACHW4Z_001430"
     CDS             complement(13446..14153)
                     /locus_tag="ACHW4Z_001430"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR8881138.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14177..14431)
                     /gene="yjbL"
                     /locus_tag="ACHW4Z_001431"
     CDS             complement(14177..14431)
                     /gene="yjbL"
                     /locus_tag="ACHW4Z_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR8881139.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="ACHW4Z_001432"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="ACHW4Z_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR8881140.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="ACHW4Z_001433"
     CDS             complement(15306..15515)
                     /locus_tag="ACHW4Z_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR8881141.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="ACHW4Z_001434"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="ACHW4Z_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR8881142.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="ACHW4Z_001435"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="ACHW4Z_001435"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR8881143.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="ACHW4Z_001436"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="ACHW4Z_001436"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR8881144.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="ACHW4Z_001437"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="ACHW4Z_001437"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR8881145.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="ACHW4Z_001438"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="ACHW4Z_001438"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR8881146.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="ACHW4Z_001439"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="ACHW4Z_001439"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR8881147.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="ACHW4Z_001440"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="ACHW4Z_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="ACHW4Z_001441"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="ACHW4Z_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR8881148.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="ACHW4Z_001442"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="ACHW4Z_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR8881149.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="ACHW4Z_001443"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="ACHW4Z_001443"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR8881150.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="ACHW4Z_001444"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="ACHW4Z_001444"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR8881151.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="ACHW4Z_001445"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="ACHW4Z_001445"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR8881152.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="ACHW4Z_001446"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="ACHW4Z_001446"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR8881153.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="ACHW4Z_001447"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="ACHW4Z_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR8881154.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="ACHW4Z_001448"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="ACHW4Z_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR8881155.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="ACHW4Z_001449"
     CDS             complement(34461..34604)
                     /locus_tag="ACHW4Z_001449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881156.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="ACHW4Z_001450"
     CDS             34605..34883
                     /locus_tag="ACHW4Z_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881157.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="ACHW4Z_001451"
     CDS             complement(34930..37026)
                     /locus_tag="ACHW4Z_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8881158.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="ACHW4Z_001452"
     CDS             complement(37026..37763)
                     /locus_tag="ACHW4Z_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8881159.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="ACHW4Z_001453"
     CDS             complement(37760..38398)
                     /locus_tag="ACHW4Z_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR8881160.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /gene="yjbE"
                     /locus_tag="ACHW4Z_001454"
     CDS             complement(38512..38754)
                     /gene="yjbE"
                     /locus_tag="ACHW4Z_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR8881161.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="ACHW4Z_001455"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="ACHW4Z_001455"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR8881162.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      41103..41299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="ACHW4Z_001456"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="ACHW4Z_001456"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR8881163.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10673 a        11029 c        10522 g        10646 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagctaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgccgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc actatgaggt agcctgagtt taacggacac tccttcctga aatagaatgg
    42841 catcagaagg agctaataat gagcagaaaa
//
LOCUS       DAZSNG010000026        40567 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZSNG010000026 DAZSNG010000000
VERSION     DAZSNG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40567
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.026"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..956)
                     /locus_tag="ACHW4Z_001457"
     CDS             complement(<1..956)
                     /locus_tag="ACHW4Z_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR8881164.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHW4Z_001458"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHW4Z_001458"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR8881165.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHW4Z_001459"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHW4Z_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHW4Z_001460"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHW4Z_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR8881166.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHW4Z_001461"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHW4Z_001461"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR8881167.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHW4Z_001462"
     CDS             complement(5018..6346)
                     /locus_tag="ACHW4Z_001462"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8881168.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="ACHW4Z_001463"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="ACHW4Z_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHW4Z_001464"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHW4Z_001464"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR8881169.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHW4Z_001465"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHW4Z_001465"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR8881170.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHW4Z_001466"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHW4Z_001466"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR8881171.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHW4Z_001467"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHW4Z_001467"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR8881172.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACHW4Z_001468"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACHW4Z_001468"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8881173.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="ACHW4Z_001469"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="ACHW4Z_001469"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR8881174.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="ACHW4Z_001470"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="ACHW4Z_001470"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR8881175.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACHW4Z_001471"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACHW4Z_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR8881176.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="ACHW4Z_001472"
     CDS             18267..19166
                     /locus_tag="ACHW4Z_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR8881177.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="ACHW4Z_001473"
     CDS             19172..21604
                     /locus_tag="ACHW4Z_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR8881178.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="ACHW4Z_001474"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="ACHW4Z_001474"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR8881179.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="ACHW4Z_001475"
     CDS             22717..23982
                     /locus_tag="ACHW4Z_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR8881180.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="ACHW4Z_001476"
     CDS             complement(24223..25815)
                     /locus_tag="ACHW4Z_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR8881181.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="ACHW4Z_001477"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="ACHW4Z_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8881182.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="ACHW4Z_001478"
     CDS             complement(27012..28130)
                     /locus_tag="ACHW4Z_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR8881183.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACHW4Z_001479"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACHW4Z_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR8881184.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="ACHW4Z_001480"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="ACHW4Z_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR8881185.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="ACHW4Z_001481"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="ACHW4Z_001481"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR8881186.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACHW4Z_001482"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACHW4Z_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR8881187.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="ACHW4Z_001483"
     CDS             complement(31379..31444)
                     /locus_tag="ACHW4Z_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881188.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="ACHW4Z_001484"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="ACHW4Z_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR8881189.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33391
                     /gene="dps"
                     /locus_tag="ACHW4Z_001485"
                     /gene_synonym="pexB"
                     /pseudo
     CDS             32865..33391
                     /gene="dps"
                     /locus_tag="ACHW4Z_001485"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
     gene            33795..34541
                     /gene="glnH"
                     /locus_tag="ACHW4Z_001486"
     CDS             33795..34541
                     /gene="glnH"
                     /locus_tag="ACHW4Z_001486"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR8881190.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34680..35339
                     /gene="glnP"
                     /locus_tag="ACHW4Z_001487"
     CDS             34680..35339
                     /gene="glnP"
                     /locus_tag="ACHW4Z_001487"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR8881191.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35336..36058
                     /gene="glnQ"
                     /locus_tag="ACHW4Z_001488"
     CDS             35336..36058
                     /gene="glnQ"
                     /locus_tag="ACHW4Z_001488"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR8881192.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36319..38543
                     /gene="ybiO"
                     /locus_tag="ACHW4Z_001489"
                     /pseudo
     CDS             36319..38543
                     /gene="ybiO"
                     /locus_tag="ACHW4Z_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38540..39466)
                     /gene="rlmF"
                     /locus_tag="ACHW4Z_001490"
     CDS             complement(38540..39466)
                     /gene="rlmF"
                     /locus_tag="ACHW4Z_001490"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR8881193.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39742..40002
                     /gene="mcbA"
                     /locus_tag="ACHW4Z_001491"
     CDS             39742..40002
                     /gene="mcbA"
                     /locus_tag="ACHW4Z_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR8881194.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40267..>40567
                     /locus_tag="ACHW4Z_001492"
     CDS             40267..>40567
                     /locus_tag="ACHW4Z_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8881195.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10103 a        10727 c         9870 g         9867 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacatacccg
    32941 caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat
    33001 ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa
    33061 cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga
    33121 taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca ctcaagttat
    33181 caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct
    33241 gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga
    33301 agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt
    33361 cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt
    33421 acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca
    33481 cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt gtatccacat
    33541 catcacacaa tcgttacata aagattgttt tttcatcagg ttttacgcta aataatcact
    33601 gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg
    33661 tattgcccta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca atgagttgca
    33721 aaattggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga
    33781 aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg accctggctt
    33841 ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg
    33901 ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg
    33961 ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca
    34021 ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg
    34081 agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg
    34141 tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg
    34201 tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc
    34261 gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg
    34321 ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca
    34381 aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg
    34441 acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca
    34501 acgaaatcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac acctgtaaag
    34561 cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc gcaaacccgc
    34621 tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata
    34681 tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa
    34741 tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag
    34801 gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc tttattgaag
    34861 tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc ctgccgatgg
    34921 cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc atgatcaact
    34981 ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac aaaggttttc
    35041 gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac gtcattttac
    35101 cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc agcattaaag
    35161 acacctcgct gtttattgtt atcggcgtgg cggaactgac ccgtcagggg caagaaatta
    35221 ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc tatctgatta
    35281 ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa atcctgtgat
    35341 tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca atatcgattt
    35401 gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg gtaaatcgac
    35461 cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga ttgtcgatgg
    35521 cctgaaggtc aacgatccga aagttgacga gcgcctgatt cgccaggaag caggtatggt
    35581 gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca tgtttggtcc
    35641 gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg agctgctggc
    35701 gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg gtggtcaaca
    35761 gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc tgtttgatga
    35821 accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta tgcaggatct
    35881 ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg ccgagaaagt
    35941 agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca atccgcaggt
    36001 gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg tctcttaata
    36061 agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc aacttattaa
    36121 aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa aaaagcccgc
    36181 acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg gaggcacacc
    36241 agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt
    36301 tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca
    36361 cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact
    36421 gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca ctggcggatg
    36481 tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact gtcgccgcga
    36541 cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg
    36601 tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg
    36661 ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca
    36721 gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga
    36781 ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg caaaaaaatc
    36841 gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt attatcgacc
    36901 tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag
    36961 gcagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat
    37021 ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca
    37081 cgattcagga cgagagcgcc cgttactggg gtcgtcgcct gagctggtta agcagcctga
    37141 ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag
    37201 gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct
    37261 tccgaaataa aaaagagatt acccagcata tgctcaactt tgcggagcat tcgctggctt
    37321 ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt
    37381 ttatcatgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa tttatgatgg
    37441 gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt
    37501 cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac tatccggagc
    37561 tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg
    37621 tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc
    37681 tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca ctcattcttt
    37741 tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg
    37801 atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta
    37861 acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg
    37921 tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt
    37981 cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa aacggcatga
    38041 acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc
    38101 gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa
    38161 ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc
    38221 gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg gaattgatgg
    38281 aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg attgtcggct
    38341 taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga
    38401 cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg
    38461 cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct gaaccgctgc
    38521 cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc tgctcgtcgt
    38581 tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta
    38641 ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac
    38701 gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca
    38761 tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc tgaccaccaa
    38821 agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc
    38881 gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt
    38941 ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc agacgaatgg
    39001 cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc
    39061 tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac
    39121 agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt
    39181 cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga
    39241 gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat
    39301 tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct gcgggtgtaa
    39361 gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc
    39421 tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta
    39481 agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat
    39541 tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg
    39601 catgtcattg atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcatttctct
    39661 gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt
    39721 tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg
    39781 agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag
    39841 ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat
    39901 aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc
    39961 ggaaatgacc agatgttagg tactgcaacc atctacaaat aaccccacca acgtaactgc
    40021 attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc
    40081 aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc atcaagtgat
    40141 aatgcttatc aaaattatta tcacttgcac gaacactatc acgggattaa cagtggcatc
    40201 gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc
    40261 tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt
    40321 gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga agggcaagct
    40381 aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa
    40441 tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt
    40501 tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgtc
    40561 cccggcg
//
LOCUS       DAZSNG010000027        39989 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZSNG010000027 DAZSNG010000000
VERSION     DAZSNG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39989
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.027"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..156
                     /locus_tag="ACHW4Z_001493"
     CDS             <1..156
                     /locus_tag="ACHW4Z_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8881196.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /gene="uidR"
                     /locus_tag="ACHW4Z_001494"
     CDS             385..975
                     /gene="uidR"
                     /locus_tag="ACHW4Z_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR8881197.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3066
                     /gene="uidA"
                     /locus_tag="ACHW4Z_001495"
     CDS             1363..3066
                     /gene="uidA"
                     /locus_tag="ACHW4Z_001495"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR8881198.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="ACHW4Z_001496"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="ACHW4Z_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="ACHW4Z_001497"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="ACHW4Z_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR8881199.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="ACHW4Z_001498"
     CDS             complement(5627..7135)
                     /locus_tag="ACHW4Z_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8881200.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="ACHW4Z_001499"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="ACHW4Z_001499"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR8881201.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /gene="fumB"
                     /locus_tag="ACHW4Z_001500"
     CDS             8610..10256
                     /gene="fumB"
                     /locus_tag="ACHW4Z_001500"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8881202.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="ACHW4Z_001501"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="ACHW4Z_001501"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR8881203.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="ACHW4Z_001502"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="ACHW4Z_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR8881204.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="ACHW4Z_001503"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="ACHW4Z_001503"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR8881205.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="ACHW4Z_001504"
     CDS             complement(14284..15603)
                     /locus_tag="ACHW4Z_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR8881206.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="ACHW4Z_001505"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="ACHW4Z_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR8881207.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="ACHW4Z_001506"
     CDS             16700..17035
                     /locus_tag="ACHW4Z_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR8881208.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="ACHW4Z_001507"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="ACHW4Z_001507"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR8881209.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="ACHW4Z_001508"
     CDS             complement(17791..19173)
                     /locus_tag="ACHW4Z_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8881210.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /gene="ydgH"
                     /locus_tag="ACHW4Z_001509"
     CDS             complement(19359..20303)
                     /gene="ydgH"
                     /locus_tag="ACHW4Z_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR8881211.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="ACHW4Z_001510"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="ACHW4Z_001510"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR8881212.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="ACHW4Z_001511"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="ACHW4Z_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR8881213.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="ACHW4Z_001512"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="ACHW4Z_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR8881214.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="ACHW4Z_001513"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="ACHW4Z_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR8881215.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="ACHW4Z_001514"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="ACHW4Z_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR8881216.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="ACHW4Z_001515"
     CDS             complement(25948..26769)
                     /locus_tag="ACHW4Z_001515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR8881217.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="ACHW4Z_001516"
     CDS             complement(26869..26952)
                     /locus_tag="ACHW4Z_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881218.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27353)
                     /gene="asr"
                     /locus_tag="ACHW4Z_001517"
     CDS             complement(27045..27353)
                     /gene="asr"
                     /locus_tag="ACHW4Z_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR8881219.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27624..27728
                     /locus_tag="ACHW4Z_001518"
     CDS             27624..27728
                     /locus_tag="ACHW4Z_001518"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR8881220.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27777..29030)
                     /locus_tag="ACHW4Z_001519"
                     /pseudo
     CDS             complement(27777..29030)
                     /locus_tag="ACHW4Z_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29137..30048
                     /locus_tag="ACHW4Z_001520"
     CDS             29137..30048
                     /locus_tag="ACHW4Z_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8881221.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30183..31403
                     /gene="mlc"
                     /locus_tag="ACHW4Z_001521"
                     /gene_synonym="dgsA"
     CDS             30183..31403
                     /gene="mlc"
                     /locus_tag="ACHW4Z_001521"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR8881222.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31528..32223
                     /gene="bioD"
                     /locus_tag="ACHW4Z_001522"
     CDS             31528..32223
                     /gene="bioD"
                     /locus_tag="ACHW4Z_001522"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8881223.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32176..33468)
                     /gene="clcB"
                     /locus_tag="ACHW4Z_001523"
     CDS             complement(32176..33468)
                     /gene="clcB"
                     /locus_tag="ACHW4Z_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR8881224.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33627..34241)
                     /gene="dmsD"
                     /locus_tag="ACHW4Z_001524"
     CDS             complement(33627..34241)
                     /gene="dmsD"
                     /locus_tag="ACHW4Z_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR8881225.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34284..35138)
                     /gene="ynfH"
                     /locus_tag="ACHW4Z_001525"
     CDS             complement(34284..35138)
                     /gene="ynfH"
                     /locus_tag="ACHW4Z_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR8881226.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35140..35757)
                     /gene="ynfG"
                     /locus_tag="ACHW4Z_001526"
     CDS             complement(35140..35757)
                     /gene="ynfG"
                     /locus_tag="ACHW4Z_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR8881227.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35768..37806)
                     /locus_tag="ACHW4Z_001527"
                     /pseudo
     CDS             complement(35768..37806)
                     /locus_tag="ACHW4Z_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37867..38901)
                     /locus_tag="ACHW4Z_001528"
                     /pseudo
     CDS             complement(37867..38901)
                     /locus_tag="ACHW4Z_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38959..>39989
                     /locus_tag="ACHW4Z_001529"
     CDS             38959..>39989
                     /locus_tag="ACHW4Z_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8881228.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10165 a         9700 c        10241 g         9883 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcagccaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa cactctcgaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggtat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 acttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgtca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacca
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttccaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccgcgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgcgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    29101 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    29161 cgttactttg ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat
    29221 atttcgcaac cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga
    29281 ctgctggcac gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca
    29341 gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat
    29401 cagggtgaag cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg
    29461 gtgtccgata cgttatcgct gtttcgccgt gattatcctg atgtccattt acaaacccgc
    29521 gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    29581 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    29641 atggcgatga tcccgtacga tcatcccctg gcaaataatc cgagtgtaac actggctgaa
    29701 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
    29761 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
    29821 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
    29881 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
    29941 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
    30001 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
    30061 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
    30121 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
    30181 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
    30241 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
    30301 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
    30361 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
    30421 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
    30481 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
    30541 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
    30601 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
    30661 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
    30721 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
    30781 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
    30841 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
    30901 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
    30961 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
    31021 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
    31081 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
    31141 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
    31201 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
    31261 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
    31321 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
    31381 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
    31441 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
    31501 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
    31561 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
    31621 aaaagggttg cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg
    31681 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
    31741 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
    31801 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
    31861 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
    31921 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
    31981 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
    32041 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
    32101 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
    32161 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
    32221 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
    32281 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
    32341 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
    32401 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
    32461 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
    32521 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
    32581 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
    32641 cccgcctagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
    32701 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
    32761 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
    32821 gtcgctatga ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg
    32881 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
    32941 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
    33001 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
    33061 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
    33121 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
    33181 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
    33241 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc
    33301 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
    33361 atgacgaaac ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag
    33421 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
    33481 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
    33541 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt
    33601 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
    33661 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
    33721 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
    33781 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
    33841 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
    33901 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
    33961 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
    34021 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
    34081 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
    34141 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
    34201 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
    34261 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
    34321 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
    34381 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
    34441 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
    34501 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
    34561 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
    34621 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
    34681 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
    34741 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
    34801 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
    34861 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
    34921 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
    34981 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
    35041 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
    35101 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
    35161 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
    35221 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
    35281 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
    35341 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
    35401 ttcagcattg tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat
    35461 acaaacatct tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg
    35521 gcagactttt gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc
    35581 gaacacgttc tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata
    35641 aatacggcgg aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca
    35701 ggttttacag ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg
    35761 ctactcctta aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca
    35821 gcggtgaagg gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac
    35881 caaacatatc cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac
    35941 gcggagtcac ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat
    36001 cgccatgacg gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc
    36061 aggcctgctg taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc
    36121 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
    36181 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
    36241 tgttggcatc aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
    36301 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
    36361 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
    36421 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg
    36481 gtcgatggca tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat
    36541 cgggaaccat tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt
    36601 tatgccgacg ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc
    36661 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
    36721 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
    36781 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
    36841 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
    36901 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
    36961 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
    37021 gcgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    37081 agtgacacca ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    37141 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    37201 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga
    37261 gttcatcaga cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc
    37321 gtacaggaca tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact
    37381 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc
    37441 gacgcgcttc atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg
    37501 cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga
    37561 ctccacccag tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca
    37621 gttaacggtg caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac
    37681 catttgggaa aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga
    37741 ggttttcatc agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat
    37801 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    37861 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    37921 tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc
    37981 agacatattg ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg
    38041 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    38101 gccgttggca atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca
    38161 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    38221 accaaacagt tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga
    38281 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    38341 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    38401 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt
    38461 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    38521 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata
    38581 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc
    38641 gaatttttcg ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc
    38701 gttaggaata atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata
    38761 ttttgccgat gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc
    38821 ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc
    38881 gtagttccag atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat
    38941 gctacttaag gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt
    39001 tctttctctg ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg
    39061 ctgacacaaa tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt
    39121 cgatatacta tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc
    39181 cgcagcacag acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag
    39241 gccttcatgt tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct
    39301 atggctgagc agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct
    39361 actctggtcg acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg
    39421 gaccagcaga agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc
    39481 gacactggtg atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac
    39541 tcaactatag gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt
    39601 ttcacacagg agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct
    39661 gagaaggtcc agaccctgaa gacatccagc tacaaggacc tctcgtcctc actcaatact
    39721 gtctcccggt tcctgcctga agacatggac ctgatgtccc gctctgggtg gctggctgtc
    39781 agggactcta tgctggcttc tgaggtgaga ccatcgacaa tcaacaagct gctgaccaaa
    39841 gcgaagatgt gtctcgatta tggcctgatg aatgggcaac ttgagggccg caaccctatt
    39901 gaacggatga agctgaccaa ggatatcgac tccaagcgca gagcgttcac agacgaggag
    39961 ctggagagac ttctggtccg tgttgagtc
//
LOCUS       DAZSNG010000028        37628 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZSNG010000028 DAZSNG010000000
VERSION     DAZSNG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.028"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..176)
                     /locus_tag="ACHW4Z_001530"
     CDS             complement(<1..176)
                     /locus_tag="ACHW4Z_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR8881229.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHW4Z_001531"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHW4Z_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8881230.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHW4Z_001532"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHW4Z_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR8881231.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHW4Z_001533"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHW4Z_001533"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR8881232.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHW4Z_001534"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHW4Z_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR8881233.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHW4Z_001535"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHW4Z_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR8881234.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACHW4Z_001536"
     CDS             complement(5180..5422)
                     /locus_tag="ACHW4Z_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR8881235.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHW4Z_001537"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHW4Z_001537"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR8881236.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHW4Z_001538"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHW4Z_001538"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR8881237.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACHW4Z_001539"
     CDS             complement(8339..9313)
                     /locus_tag="ACHW4Z_001539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR8881238.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACHW4Z_001540"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACHW4Z_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR8881239.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACHW4Z_001541"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACHW4Z_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR8881240.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACHW4Z_001542"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACHW4Z_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR8881241.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACHW4Z_001543"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACHW4Z_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR8881242.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHW4Z_001544"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHW4Z_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR8881243.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACHW4Z_001545"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACHW4Z_001545"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR8881244.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHW4Z_001546"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHW4Z_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR8881245.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACHW4Z_001547"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACHW4Z_001547"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR8881246.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHW4Z_001548"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHW4Z_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR8881247.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHW4Z_001549"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHW4Z_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR8881248.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHW4Z_001550"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHW4Z_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR8881249.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHW4Z_001551"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHW4Z_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR8881250.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACHW4Z_001552"
     CDS             26388..26660
                     /locus_tag="ACHW4Z_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR8881251.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHW4Z_001553"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHW4Z_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR8881252.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACHW4Z_001554"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACHW4Z_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR8881253.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="ACHW4Z_001555"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="ACHW4Z_001555"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8881254.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACHW4Z_001556"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACHW4Z_001556"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR8881255.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="ACHW4Z_001557"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="ACHW4Z_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /gene="zapG"
                     /locus_tag="ACHW4Z_001558"
     CDS             complement(31907..32305)
                     /gene="zapG"
                     /locus_tag="ACHW4Z_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR8881256.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="ACHW4Z_001559"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="ACHW4Z_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR8881257.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="ACHW4Z_001560"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="ACHW4Z_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR8881258.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="ACHW4Z_001561"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="ACHW4Z_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR8881259.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="ACHW4Z_001562"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="ACHW4Z_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR8881260.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="ACHW4Z_001563"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="ACHW4Z_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR8881261.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="ACHW4Z_001564"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="ACHW4Z_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8881262.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9116 a         9414 c         9900 g         9198 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAZSNG010000029        36587 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZSNG010000029 DAZSNG010000000
VERSION     DAZSNG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.029"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..190
                     /locus_tag="ACHW4Z_001565"
     CDS             <1..190
                     /locus_tag="ACHW4Z_001565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881263.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHW4Z_001566"
     CDS             complement(156..1391)
                     /locus_tag="ACHW4Z_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8881264.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHW4Z_001567"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHW4Z_001567"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHW4Z_001568"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHW4Z_001568"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHW4Z_001569"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHW4Z_001569"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHW4Z_001570"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHW4Z_001570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHW4Z_001571"
     CDS             complement(2077..3462)
                     /locus_tag="ACHW4Z_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8881265.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHW4Z_001572"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHW4Z_001572"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR8881266.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHW4Z_001573"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHW4Z_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR8881267.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHW4Z_001574"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHW4Z_001574"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR8881268.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHW4Z_001575"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHW4Z_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR8881269.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHW4Z_001576"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHW4Z_001576"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR8881270.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHW4Z_001577"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHW4Z_001577"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR8881271.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHW4Z_001578"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHW4Z_001578"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8881272.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHW4Z_001579"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHW4Z_001579"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8881273.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHW4Z_001580"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHW4Z_001580"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR8881274.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHW4Z_001581"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHW4Z_001581"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR8881275.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHW4Z_001582"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHW4Z_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR8881276.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHW4Z_001583"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHW4Z_001583"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR8881277.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHW4Z_001584"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHW4Z_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR8881278.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHW4Z_001585"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHW4Z_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR8881279.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHW4Z_001586"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHW4Z_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR8881280.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHW4Z_001587"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHW4Z_001587"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR8881281.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHW4Z_001588"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHW4Z_001588"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR8881282.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHW4Z_001589"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHW4Z_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR8881283.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHW4Z_001590"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHW4Z_001590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR8881284.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHW4Z_001591"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHW4Z_001591"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR8881285.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHW4Z_001592"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHW4Z_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR8881286.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHW4Z_001593"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHW4Z_001593"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR8881287.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHW4Z_001594"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHW4Z_001594"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR8881288.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHW4Z_001595"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHW4Z_001595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR8881289.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHW4Z_001596"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHW4Z_001596"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR8881290.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHW4Z_001597"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHW4Z_001597"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR8881291.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHW4Z_001598"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHW4Z_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR8881292.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHW4Z_001599"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHW4Z_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR8881293.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHW4Z_001600"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHW4Z_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHW4Z_001601"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHW4Z_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR8881294.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHW4Z_001602"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHW4Z_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR8881295.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHW4Z_001603"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHW4Z_001603"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHW4Z_001604"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHW4Z_001604"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8940 a        10012 c         9217 g         8418 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZSNG010000030        34999 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZSNG010000030 DAZSNG010000000
VERSION     DAZSNG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.030"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..815)
                     /locus_tag="ACHW4Z_001605"
     CDS             complement(<1..815)
                     /locus_tag="ACHW4Z_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8881296.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHW4Z_001606"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHW4Z_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHW4Z_001607"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHW4Z_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACHW4Z_001608"
     CDS             complement(1398..1874)
                     /locus_tag="ACHW4Z_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR8881297.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHW4Z_001609"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHW4Z_001609"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR8881298.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACHW4Z_001610"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACHW4Z_001610"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR8881299.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACHW4Z_001611"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACHW4Z_001611"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR8881300.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACHW4Z_001612"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACHW4Z_001612"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR8881301.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACHW4Z_001613"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACHW4Z_001613"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8881302.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHW4Z_001614"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHW4Z_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR8881303.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPEALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHW4Z_001615"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHW4Z_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR8881304.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="ACHW4Z_001616"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="ACHW4Z_001616"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR8881305.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="ACHW4Z_001617"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="ACHW4Z_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR8881306.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="ACHW4Z_001618"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="ACHW4Z_001618"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR8881307.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="ACHW4Z_001619"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="ACHW4Z_001619"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR8881308.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="ACHW4Z_001620"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="ACHW4Z_001620"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR8881309.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="ACHW4Z_001621"
     CDS             13849..14553
                     /locus_tag="ACHW4Z_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8881310.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="ACHW4Z_001622"
     CDS             14758..15471
                     /locus_tag="ACHW4Z_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8881311.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="ACHW4Z_001623"
     CDS             complement(15507..16463)
                     /locus_tag="ACHW4Z_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR8881312.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHW4Z_001624"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHW4Z_001624"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR8881313.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="ACHW4Z_001625"
     CDS             complement(17701..18462)
                     /locus_tag="ACHW4Z_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8881314.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="ACHW4Z_001626"
     CDS             18595..19005
                     /locus_tag="ACHW4Z_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR8881315.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="ACHW4Z_001627"
     CDS             complement(18967..20073)
                     /locus_tag="ACHW4Z_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8881316.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="ACHW4Z_001628"
     CDS             complement(20084..21217)
                     /locus_tag="ACHW4Z_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8881317.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="ACHW4Z_001629"
     CDS             complement(21210..22946)
                     /locus_tag="ACHW4Z_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8881318.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHW4Z_001630"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHW4Z_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR8881319.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHW4Z_001631"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHW4Z_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR8881320.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHW4Z_001632"
     CDS             24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHW4Z_001632"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR8881321.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26051..26149)
                     /locus_tag="ACHW4Z_001633"
                     /pseudo
     CDS             complement(26051..26149)
                     /locus_tag="ACHW4Z_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHW4Z_001634"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHW4Z_001634"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR8881322.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="ACHW4Z_001635"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="ACHW4Z_001635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR8881323.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHW4Z_001636"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHW4Z_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR8881324.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHW4Z_001637"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHW4Z_001637"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR8881325.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHW4Z_001638"
     CDS             31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHW4Z_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR8881326.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHW4Z_001639"
     CDS             complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHW4Z_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR8881327.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHW4Z_001640"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHW4Z_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR8881328.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHW4Z_001641"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHW4Z_001641"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR8881329.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34999)
                     /locus_tag="ACHW4Z_001642"
                     /pseudo
     CDS             complement(33012..34999)
                     /locus_tag="ACHW4Z_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8294 a         8973 c         9457 g         8275 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccggaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccatagtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgccgg
//
LOCUS       DAZSNG010000031        34788 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZSNG010000031 DAZSNG010000000
VERSION     DAZSNG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.031"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2098
                     /locus_tag="ACHW4Z_001643"
     CDS             <1..2098
                     /locus_tag="ACHW4Z_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR8881330.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHW4Z_001644"
     CDS             2095..3183
                     /locus_tag="ACHW4Z_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR8881331.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHW4Z_001645"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHW4Z_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR8881332.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHW4Z_001646"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHW4Z_001646"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR8881333.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHW4Z_001647"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHW4Z_001647"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR8881334.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHW4Z_001648"
     CDS             8051..8602
                     /locus_tag="ACHW4Z_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR8881335.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHW4Z_001649"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHW4Z_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR8881336.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHW4Z_001650"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHW4Z_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR8881337.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHW4Z_001651"
     CDS             complement(11683..12681)
                     /locus_tag="ACHW4Z_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR8881338.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHW4Z_001652"
     CDS             complement(12760..13452)
                     /locus_tag="ACHW4Z_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR8881339.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHW4Z_001653"
     CDS             complement(13529..14032)
                     /locus_tag="ACHW4Z_001653"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR8881340.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHW4Z_001654"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHW4Z_001654"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR8881341.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHW4Z_001655"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHW4Z_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR8881342.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHW4Z_001656"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHW4Z_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR8881343.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHW4Z_001657"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHW4Z_001657"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR8881344.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHW4Z_001658"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHW4Z_001658"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR8881345.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHW4Z_001659"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHW4Z_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHW4Z_001660"
     CDS             complement(22127..23560)
                     /locus_tag="ACHW4Z_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8881346.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHW4Z_001661"
     CDS             complement(23623..24072)
                     /locus_tag="ACHW4Z_001661"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR8881347.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHW4Z_001662"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHW4Z_001662"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR8881348.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHW4Z_001663"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHW4Z_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR8881349.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHW4Z_001664"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHW4Z_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHW4Z_001665"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHW4Z_001665"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR8881350.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHW4Z_001666"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHW4Z_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHW4Z_001667"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHW4Z_001667"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR8881351.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHW4Z_001668"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHW4Z_001668"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(34082..34157)
                     /locus_tag="ACHW4Z_001669"
     tRNA            complement(34082..34157)
                     /locus_tag="ACHW4Z_001669"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="ACHW4Z_001670"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="ACHW4Z_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8484 a         8944 c         9141 g         8219 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccattaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcacaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DAZSNG010000032        34446 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZSNG010000032 DAZSNG010000000
VERSION     DAZSNG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.032"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..333)
                     /locus_tag="ACHW4Z_001671"
     CDS             complement(<1..333)
                     /locus_tag="ACHW4Z_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881352.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            495..551
                     /gene="ysgD"
                     /locus_tag="ACHW4Z_001672"
     CDS             495..551
                     /gene="ysgD"
                     /locus_tag="ACHW4Z_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR8881353.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="ACHW4Z_001673"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="ACHW4Z_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR8881354.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="ACHW4Z_001674"
     CDS             complement(1694..2074)
                     /locus_tag="ACHW4Z_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR8881355.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2468)
                     /gene="yigG"
                     /locus_tag="ACHW4Z_001675"
     CDS             complement(2088..2468)
                     /gene="yigG"
                     /locus_tag="ACHW4Z_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR8881356.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="ACHW4Z_001676"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="ACHW4Z_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR8881357.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /gene="yigI"
                     /locus_tag="ACHW4Z_001677"
     CDS             complement(3505..3972)
                     /gene="yigI"
                     /locus_tag="ACHW4Z_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR8881358.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="ACHW4Z_001678"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="ACHW4Z_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR8881359.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="ACHW4Z_001679"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="ACHW4Z_001679"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR8881360.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="ACHW4Z_001680"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="ACHW4Z_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR8881361.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="ACHW4Z_001681"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="ACHW4Z_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR8881362.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="ACHW4Z_001682"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="ACHW4Z_001682"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR8881363.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /gene="yigL"
                     /locus_tag="ACHW4Z_001683"
     CDS             9475..10275
                     /gene="yigL"
                     /locus_tag="ACHW4Z_001683"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR8881364.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="ACHW4Z_001684"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="ACHW4Z_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR8881365.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="ACHW4Z_001685"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="ACHW4Z_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR8881366.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="ACHW4Z_001686"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="ACHW4Z_001686"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR8881367.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="ACHW4Z_001687"
     CDS             complement(14627..15442)
                     /locus_tag="ACHW4Z_001687"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR8881368.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="ACHW4Z_001688"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="ACHW4Z_001688"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR8881369.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="ACHW4Z_001689"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="ACHW4Z_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR8881370.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="ACHW4Z_001690"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="ACHW4Z_001690"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR8881371.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="ACHW4Z_001691"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="ACHW4Z_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR8881372.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="ACHW4Z_001692"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="ACHW4Z_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR8881373.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="ACHW4Z_001693"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="ACHW4Z_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR8881374.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="ACHW4Z_001694"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="ACHW4Z_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR8881375.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="ACHW4Z_001695"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="ACHW4Z_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR8881376.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..23609
                     /gene="tatD"
                     /locus_tag="ACHW4Z_001696"
     CDS             22827..23609
                     /gene="tatD"
                     /locus_tag="ACHW4Z_001696"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR8881377.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="ACHW4Z_001697"
     CDS             complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="ACHW4Z_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR8881378.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24261..25754
                     /gene="ubiD"
                     /locus_tag="ACHW4Z_001698"
     CDS             24261..25754
                     /gene="ubiD"
                     /locus_tag="ACHW4Z_001698"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR8881379.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25800..26501
                     /gene="fre"
                     /locus_tag="ACHW4Z_001699"
     CDS             25800..26501
                     /gene="fre"
                     /locus_tag="ACHW4Z_001699"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR8881380.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="ACHW4Z_001700"
     CDS             complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="ACHW4Z_001700"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR8881381.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="ACHW4Z_001701"
     CDS             complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="ACHW4Z_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR8881382.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30245..31576
                     /gene="pepQ"
                     /locus_tag="ACHW4Z_001702"
     CDS             30245..31576
                     /gene="pepQ"
                     /locus_tag="ACHW4Z_001702"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR8881383.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31576..32190
                     /locus_tag="ACHW4Z_001703"
     CDS             31576..32190
                     /locus_tag="ACHW4Z_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR8881384.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32229..33680
                     /gene="trkH"
                     /locus_tag="ACHW4Z_001704"
     CDS             32229..33680
                     /gene="trkH"
                     /locus_tag="ACHW4Z_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR8881385.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33692..34237
                     /gene="hemG"
                     /locus_tag="ACHW4Z_001705"
     CDS             33692..34237
                     /gene="hemG"
                     /locus_tag="ACHW4Z_001705"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR8881386.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8698 c         9302 g         8338 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctagatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggtggctt agcctggcta
     1681 agccacctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggtcacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcatcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aatttcctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acttggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gctctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    23041 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    23101 gtggtctcga ctttaaccgt aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    23161 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    23221 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    23281 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    23341 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    23401 tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc
    23461 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    23521 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    23581 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    23641 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    23701 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    23761 gataaggggg ggccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatta
    23821 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    23881 tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    23941 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    24001 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    24061 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    24121 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    24181 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    24241 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    24301 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    24361 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    24421 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    24481 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    24541 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    24601 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    24661 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    24721 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    24781 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    24841 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    24901 gtttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    24961 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    25021 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    25081 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    25141 actataatga agtcgataat ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    25201 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    25261 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    25321 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    25381 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    25441 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    25501 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    25561 ctattgatta tctggatttt gcctcgcctg tctctgggct gggttcaaaa atggggctgg
    25621 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    25681 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    25741 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    25801 tgacaacctt aagctgtaaa gtgacctcgg tagcagctat cacggatacc gtatatcgtg
    25861 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    25921 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    25981 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    26041 tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg
    26101 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    26161 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    26221 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccttg
    26281 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    26341 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    26401 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    26461 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc
    26521 ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc
    26581 atccggcaat tggtgcacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt
    26641 ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    26701 gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact
    26761 gaacgtcttt gcgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    26821 ccagcggatg acccagcgcg atcgcaccac cattgaggtt gatcttctcg tcaatctgct
    26881 ccattagccc gagatcttta atgcacggca ggatctgcgc ggcaaaggct tcgttcatct
    26941 caaacacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    27001 gaaccgggcc gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac
    27061 gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag
    27121 ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa
    27181 acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    27241 aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt
    27301 taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat
    27361 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    27421 tgcccgccgc tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca
    27481 tcggcacatg gcctatatgc tccacgccgc caaccagaca tgcctgcgta tcgccagtca
    27541 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacgg
    27601 taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa
    27661 aaccctgctc cagcgtctgt tgcacacaac cccagtaaat atcgtcgagg gccgccgctt
    27721 ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    27781 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    27841 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    27901 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    27961 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    28021 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    28081 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcgcacca
    28141 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    28201 tcggttggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    28261 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    28321 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    28381 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacaacg tccagcagat
    28441 atgccgggcc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    28501 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    28561 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    28621 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    28681 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccggg cgttccagcg
    28741 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatcctggc
    28801 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcaacaaccg
    28861 cttctaccac aatatccacg cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg
    28921 agatcacgcc agccagtttc agaccatcga ttttgccgcg ctcaagctgc ttgttcagca
    28981 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    29041 ccggcacgcc tttccacgcg gactggtaag cgataccgcc acccatgatg cctgcgccca
    29101 gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    29161 catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg
    29221 ggacaaaact tttgttttcc agatttaagg cttcttcacg accaaaacgg gccgcagctt
    29281 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgcgcgacca
    29341 ttcctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcct
    29401 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    29461 ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    29521 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc
    29581 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    29641 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    29701 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    29761 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    29821 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    29881 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    29941 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    30001 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    30061 tctcctgaat ccgcaaggta atctggtcgt accagatgca tcggagtgtg tgttttgtgt
    30121 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    30181 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    30241 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    30301 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    30361 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    30421 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    30481 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    30541 ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    30601 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    30661 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attattaccg
    30721 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    30781 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaatat
    30841 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    30901 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    30961 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    31021 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    31081 tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccacat
    31141 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    31201 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtattggcca
    31261 tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacaca
    31321 cctcgcggca ccggcaaaat atccgtacct gcgctacacc cgtattctcc agccgggcat
    31381 ggtgttaacc atcgaaccgg gtatctactt cattgaatcg cttctggcac cgtggcgtga
    31441 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    31501 cattcgtatc gaagacaacg tggtgatcca cgaaaacaac gtggaaaaca tgacccggga
    31561 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    31621 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    31681 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    31741 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    31801 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    31861 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    31921 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    31981 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    32041 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    32101 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    32161 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    32221 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    32281 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    32341 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    32401 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    32461 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    32521 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    32581 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    32641 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    32701 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    32761 ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    32821 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    32881 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    32941 ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt
    33001 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    33061 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgtct
    33121 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    33181 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    33241 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    33301 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    33361 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    33421 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    33481 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    33541 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    33601 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attactggtg ctctttaccc
    33661 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    33721 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    33781 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    33841 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    33901 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    33961 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    34021 gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    34081 ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg aagatgtcag gcggtgaaac
    34141 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    34201 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    34261 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    34321 ccactgacac ggaacaacgg caaacacgca gccgggtcag cggggttctc ctgagaattc
    34381 cgacagagaa agcaaaaata aatgcttgac tttgtagcgg gaaagcgtat tatgaacacc
    34441 ctgcgc
//
LOCUS       DAZSNG010000033        33534 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZSNG010000033 DAZSNG010000000
VERSION     DAZSNG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33534
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.033"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHW4Z_001706"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHW4Z_001706"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR8881387.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHW4Z_001707"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHW4Z_001707"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR8881388.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHW4Z_001708"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHW4Z_001708"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR8881389.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHW4Z_001709"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHW4Z_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR8881390.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHW4Z_001710"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHW4Z_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR8881391.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHW4Z_001711"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHW4Z_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8881392.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHW4Z_001712"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHW4Z_001712"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR8881393.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHW4Z_001713"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHW4Z_001713"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR8881394.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHW4Z_001714"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHW4Z_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR8881395.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHW4Z_001715"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHW4Z_001715"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR8881396.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHW4Z_001716"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHW4Z_001716"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR8881397.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHW4Z_001717"
     CDS             8212..9075
                     /locus_tag="ACHW4Z_001717"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR8881398.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHW4Z_001718"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHW4Z_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR8881399.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHW4Z_001719"
     CDS             10411..11028
                     /locus_tag="ACHW4Z_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR8881400.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHW4Z_001720"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHW4Z_001720"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR8881401.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHW4Z_001721"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHW4Z_001721"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR8881402.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHW4Z_001722"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHW4Z_001722"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR8881403.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHW4Z_001723"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHW4Z_001723"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR8881404.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHW4Z_001724"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHW4Z_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR8881405.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHW4Z_001725"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHW4Z_001725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR8881406.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHW4Z_001726"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHW4Z_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR8881407.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHW4Z_001727"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHW4Z_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR8881408.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHW4Z_001728"
     CDS             21859..23568
                     /locus_tag="ACHW4Z_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8881409.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHW4Z_001729"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHW4Z_001729"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR8881410.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHW4Z_001730"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHW4Z_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHW4Z_001731"
     tRNA            27625..27719
                     /locus_tag="ACHW4Z_001731"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHW4Z_001732"
     CDS             27991..29202
                     /locus_tag="ACHW4Z_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8881411.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="ACHW4Z_001733"
     CDS             29614..30657
                     /locus_tag="ACHW4Z_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8881412.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="ACHW4Z_001734"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="ACHW4Z_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="ACHW4Z_001735"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="ACHW4Z_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            33237..33494
                     /locus_tag="ACHW4Z_001736"
     CDS             33237..33494
                     /locus_tag="ACHW4Z_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881413.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         8402 a         8816 c         8262 g         8054 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta cttgaaccgc cccg
//
LOCUS       DAZSNG010000034        33447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZSNG010000034 DAZSNG010000000
VERSION     DAZSNG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.035"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHW4Z_001737"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHW4Z_001737"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR8881414.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHW4Z_001738"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHW4Z_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR8881415.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHW4Z_001739"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHW4Z_001739"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR8881416.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHW4Z_001740"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHW4Z_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR8881417.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHW4Z_001741"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHW4Z_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR8881418.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHW4Z_001742"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHW4Z_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR8881419.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHW4Z_001743"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHW4Z_001743"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR8881420.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="ACHW4Z_001744"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="ACHW4Z_001744"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR8881421.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="ACHW4Z_001745"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="ACHW4Z_001745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR8881422.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="ACHW4Z_001746"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="ACHW4Z_001746"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR8881423.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="ACHW4Z_001747"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="ACHW4Z_001747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR8881424.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="ACHW4Z_001748"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="ACHW4Z_001748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR8881425.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="ACHW4Z_001749"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="ACHW4Z_001749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR8881426.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="ACHW4Z_001750"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="ACHW4Z_001750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR8881427.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="ACHW4Z_001751"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="ACHW4Z_001751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR8881428.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /locus_tag="ACHW4Z_001752"
     CDS             15215..15634
                     /locus_tag="ACHW4Z_001752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR8881429.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="ACHW4Z_001753"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="ACHW4Z_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR8881430.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="ACHW4Z_001754"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="ACHW4Z_001754"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR8881431.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="ACHW4Z_001755"
     CDS             19508..20329
                     /locus_tag="ACHW4Z_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR8881432.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="ACHW4Z_001756"
     CDS             20316..22424
                     /locus_tag="ACHW4Z_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR8881433.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="ACHW4Z_001757"
     CDS             22421..24088
                     /locus_tag="ACHW4Z_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8881434.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="ACHW4Z_001758"
     CDS             24091..25617
                     /locus_tag="ACHW4Z_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR8881435.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..27234
                     /locus_tag="ACHW4Z_001759"
     CDS             25618..27234
                     /locus_tag="ACHW4Z_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR8881436.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="ACHW4Z_001760"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="ACHW4Z_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27465..27842)
                     /locus_tag="ACHW4Z_001761"
     CDS             complement(27465..27842)
                     /locus_tag="ACHW4Z_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881437.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="ACHW4Z_001762"
     CDS             complement(28252..28623)
                     /locus_tag="ACHW4Z_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881438.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="ACHW4Z_001763"
     CDS             complement(28684..29181)
                     /locus_tag="ACHW4Z_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881439.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29210..30045)
                     /gene="aadA1"
                     /locus_tag="ACHW4Z_001764"
                     /pseudo
     CDS             complement(29210..30045)
                     /gene="aadA1"
                     /locus_tag="ACHW4Z_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="ACHW4Z_001765"
     CDS             complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="ACHW4Z_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8881440.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30722..31195)
                     /gene="dfrA1"
                     /locus_tag="ACHW4Z_001766"
     CDS             complement(30722..31195)
                     /gene="dfrA1"
                     /locus_tag="ACHW4Z_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8881441.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31527..32504
                     /gene="intI2"
                     /locus_tag="ACHW4Z_001767"
                     /pseudo
     CDS             31527..32504
                     /gene="intI2"
                     /locus_tag="ACHW4Z_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(32692..32931)
                     /locus_tag="ACHW4Z_001768"
     CDS             complement(32692..32931)
                     /locus_tag="ACHW4Z_001768"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR8881442.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8675 a         7805 c         8446 g         8521 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttatgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag
    29401 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
    29461 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
    29521 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
    29581 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
    29641 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
    29701 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
    29761 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
    29821 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
    29881 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
    29941 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
    30001 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg
    30061 aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc
    30121 tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata
    30181 cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg
    30241 tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta gtgcccactt
    30301 tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac
    30361 aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct tcccgacaag
    30421 ctcttggtcg atgaatgcgc catagcaagc agagtcttca tcagagtcat catccgagat
    30481 gtaatccttc cggtaggggc tcacacttct ggtagatagt tcaaagcctt ggtcggatag
    30541 gtgcacatcg aacacttcac gaacaatgaa atggttctca gcatccaatg tttccgccac
    30601 ctgctcaggg atcaccgaaa tcttcatatg acgcctaacg cctggcacag cggatcgcaa
    30661 acctggcgcg gcttttggta caaaaggcgt gacaggtttg cgaatccgtt gctgccactt
    30721 gttaaccctt ttgccagatt tggtaactat aatttatgtt agaggcgaag tcttgggtaa
    30781 aaactggcct aaaattgctg gggatttcag gaaagtaaac atcaccttcc ggctcgatgt
    30841 ctattgtaga tatatgtagt gtatctactt gatcgatcag gcttttgtat atctccccac
    30901 cacctgaaac aatgacatga tccgttattt tctttaggtt ggttaaagca tctttaattg
    30961 atggaaagat caatacgttc tcattgtcag atgtaaaact tgaacgtgtt acgaccgcat
    31021 actttcggtt gggtaatgct cccattgatt caaaagtctt gcgtccaacc aacagccatt
    31081 ggttataggt aatagcttta aacaggagct gttcaccttt ggcactccat ggaatatcag
    31141 ggccattccc gataactcca ttcttcgata tagctaccat tagtgatagt ttcacaattc
    31201 ttcctcagag gttaacattt tattagtaag catgctcgtt ttgtcacccg ctgatgctta
    31261 ccgttaatta atataatcac ttgtattaaa tagactttct ttaaaaatac aagacactct
    31321 gttattacaa atcgtgcatg ccgtctatcc tgcttgcacg atgcacatta tctcacccaa
    31381 agaactcata acattttaat ttatttagca ttaaattaaa actctaccgg taaacaagca
    31441 tctctaggcg taaaaaacag gcactttttt aaatcccagc tagaataggc tgtataggca
    31501 gacagttgca agacaaggag cgttttatgt ctaacagtcc atttttaaat tctatacgca
    31561 cggatatgcg acaaaaaggt tatgcgctga aaactgaaaa aacttacctg cactggatta
    31621 agcgttttat tctgtttcac aaaaaacgtc atcctcagac catgggcagt gaagaggtca
    31681 ggctgttttt atccagctta gcaaacagca gacatgtagc cataaacacg cagaaaatcg
    31741 ctttaaatgc cctagctttt ttgtacaaca ggtttttaca acagccgttg ggcgatattg
    31801 attatatccc tgcaagcaag cctagacggc taccctctgt tatctctgca aatgaagtgc
    31861 aacgcatttt gcaggttatg gatactcgca accaagttat ttttacgctg ctgtatggtg
    31921 caggtttgcg cattaatgaa tgcttgcgtt tgcgggttaa agattttgat tttgataatg
    31981 gctgcatcac tgtgcatgac ggtaagggtg ggaaaagcag aaacagccta ctgcccacgc
    32041 gcctaatccc agcaataaaa taactcattg agcaagcgcg gcttattcag caagacgaca
    32101 acttacaagg cgtagggcca tcgctgcctt ttgctttaga tcacaaatac ccttctgctt
    32161 atcgacaagc ggcgtggatg tttgtctttc cctccagcac gctctgcaac cacccgtata
    32221 acggcaaatt atgccgccat catctgcatg actccgttgc gcgaaaggca ttgaaggcag
    32281 ccgtacaaaa agcaggcatc gttagcaagc gtgtcacttg tcatacattt cgtcactcgt
    32341 ttgctacgca tctattacaa gcggggcgtg atattcgcac tgtgcaagaa ctcttagggc
    32401 ataacgatgt taagaccacg caaatctata cgcatgtgtt gggtcagcat tttgccggca
    32461 ccaccagtcc tgcggatgga ctgatgctac ttatcaatca gtaaaagtag ataaatatct
    32521 ttatgtagcc ctgagtagcc cctgatactt tttatggtga aaaagataaa caatcataga
    32581 ggggataaat atgagccagt tctaccattg ttcagataag tttaagcaag atactgtcaa
    32641 tcacagtgac cattcatggt cattacactt cctccagttg ccagacaatg cctaacataa
    32701 aaacgcaaaa gcccaaaagt cagagcttag gtgttctagg cgttttctaa gcgctgaaga
    32761 aatacgtctt accgtagatt gcgtgcgttt ctgaagcaca ttttccttat acatggcttt
    32821 atcccaggca gcttcatcaa gctcttggag taacaagcca gtaataatgc gacactctcg
    32881 ggtttgtagc gagccgctgg tgagatcgga gttatagcgg aaaggggtca tttgtaatta
    32941 tttgtgggtg gaggatagtt aacttattat tgtagttgga agaggctgct ttccaaacat
    33001 tagcacctcg taatgcttgt tactgtatat taatttatgc tcttagcgca tgttaatggg
    33061 ctattacaat gcataatgtc cacatcaaga taactgtggc ttaacacctg tacaagcgaa
    33121 aaaaatcaat cacctgtccg gaaatacggg atcactgtaa agtgttggtg aggcagtcta
    33181 ggagggggat ttaatttggg atactgatga aaggattatg atcagttgga tataggatcg
    33241 tactaagctg agcggcaggt tagctagatt agttaaccag ataagtgaaa tctagttcca
    33301 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
    33361 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
    33421 aactattttg tccgcccaca gctgccg
//
LOCUS       DAZSNG010000035        31186 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZSNG010000035 DAZSNG010000000
VERSION     DAZSNG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.036"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            389..2047
                     /locus_tag="ACHW4Z_001769"
     CDS             389..2047
                     /locus_tag="ACHW4Z_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR8881443.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHW4Z_001770"
     CDS             complement(2044..3000)
                     /locus_tag="ACHW4Z_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR8881444.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHW4Z_001771"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHW4Z_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR8881445.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHW4Z_001772"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHW4Z_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR8881446.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHW4Z_001773"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHW4Z_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR8881447.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHW4Z_001774"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHW4Z_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR8881448.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHW4Z_001775"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHW4Z_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR8881449.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHW4Z_001776"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHW4Z_001776"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR8881450.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHW4Z_001777"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHW4Z_001777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHW4Z_001778"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHW4Z_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR8881451.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHW4Z_001779"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHW4Z_001779"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR8881452.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHW4Z_001780"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHW4Z_001780"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR8881453.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHW4Z_001781"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHW4Z_001781"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR8881454.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHW4Z_001782"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHW4Z_001782"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR8881455.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHW4Z_001783"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHW4Z_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR8881456.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHW4Z_001784"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHW4Z_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR8881457.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHW4Z_001785"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHW4Z_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR8881458.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHW4Z_001786"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHW4Z_001786"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR8881459.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHW4Z_001787"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHW4Z_001787"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR8881460.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHW4Z_001788"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHW4Z_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHW4Z_001789"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHW4Z_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR8881461.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHW4Z_001790"
     CDS             28042..28833
                     /locus_tag="ACHW4Z_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR8881462.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHW4Z_001791"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHW4Z_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR8881463.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHW4Z_001792"
     CDS             29810..30958
                     /locus_tag="ACHW4Z_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881464.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31186
                     /locus_tag="ACHW4Z_001793"
     CDS             30976..>31186
                     /locus_tag="ACHW4Z_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8881465.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7689 a         7645 c         8374 g         7478 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagg
//
LOCUS       DAZSNG010000036        30666 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZSNG010000036 DAZSNG010000000
VERSION     DAZSNG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.037"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACHW4Z_001794"
     CDS             <1..588
                     /locus_tag="ACHW4Z_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR8881466.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACHW4Z_001795"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACHW4Z_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR8881467.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACHW4Z_001796"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACHW4Z_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR8881468.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACHW4Z_001797"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACHW4Z_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR8881469.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACHW4Z_001798"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACHW4Z_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR8881470.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHW4Z_001799"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHW4Z_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR8881471.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACHW4Z_001800"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACHW4Z_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR8881472.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHW4Z_001801"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHW4Z_001801"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR8881473.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHW4Z_001802"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHW4Z_001802"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR8881474.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHW4Z_001803"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHW4Z_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR8881475.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHW4Z_001804"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHW4Z_001804"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR8881476.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHW4Z_001805"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHW4Z_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR8881477.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHW4Z_001806"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHW4Z_001806"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR8881478.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHW4Z_001807"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHW4Z_001807"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR8881479.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHW4Z_001808"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHW4Z_001808"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR8881480.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHW4Z_001809"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHW4Z_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR8881481.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACHW4Z_001810"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACHW4Z_001810"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR8881482.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACHW4Z_001811"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACHW4Z_001811"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR8881483.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACHW4Z_001812"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACHW4Z_001812"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR8881484.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACHW4Z_001813"
     CDS             22192..22782
                     /locus_tag="ACHW4Z_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR8881485.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACHW4Z_001814"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACHW4Z_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR8881486.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACHW4Z_001815"
     CDS             23254..23949
                     /locus_tag="ACHW4Z_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR8881487.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHW4Z_001816"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHW4Z_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR8881488.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACHW4Z_001817"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACHW4Z_001817"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR8881489.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACHW4Z_001818"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACHW4Z_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR8881490.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHW4Z_001819"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHW4Z_001819"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR8881491.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHW4Z_001820"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHW4Z_001820"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR8881492.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7189 a         7983 c         8440 g         7054 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgattg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgccgcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAZSNG010000037        30665 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZSNG010000037 DAZSNG010000000
VERSION     DAZSNG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30665
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.038"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHW4Z_001821"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHW4Z_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR8881493.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACHW4Z_001822"
     CDS             complement(1899..2636)
                     /locus_tag="ACHW4Z_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881494.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHW4Z_001823"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHW4Z_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR8881495.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACHW4Z_001824"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACHW4Z_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR8881496.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACHW4Z_001825"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACHW4Z_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR8881497.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACHW4Z_001826"
     CDS             complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACHW4Z_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR8881498.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACHW4Z_001827"
     CDS             complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACHW4Z_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR8881499.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACHW4Z_001828"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACHW4Z_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR8881500.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACHW4Z_001829"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACHW4Z_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR8881501.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACHW4Z_001830"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACHW4Z_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR8881502.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="ACHW4Z_001831"
     CDS             9819..10154
                     /locus_tag="ACHW4Z_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR8881503.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACHW4Z_001832"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACHW4Z_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR8881504.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACHW4Z_001833"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACHW4Z_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR8881505.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /locus_tag="ACHW4Z_001834"
     CDS             complement(11226..11483)
                     /locus_tag="ACHW4Z_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR8881506.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACHW4Z_001835"
     CDS             complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACHW4Z_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR8881507.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="ACHW4Z_001836"
     CDS             complement(13650..14030)
                     /locus_tag="ACHW4Z_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881508.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACHW4Z_001837"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACHW4Z_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR8881509.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="ACHW4Z_001838"
     CDS             complement(14674..14979)
                     /locus_tag="ACHW4Z_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881510.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACHW4Z_001839"
     CDS             complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACHW4Z_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR8881511.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..16630)
                     /gene="traW"
                     /locus_tag="ACHW4Z_001840"
     CDS             complement(15998..16630)
                     /gene="traW"
                     /locus_tag="ACHW4Z_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR8881512.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="ACHW4Z_001841"
     CDS             complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="ACHW4Z_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR8881513.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17010..19640)
                     /gene="traC"
                     /locus_tag="ACHW4Z_001842"
     CDS             complement(17010..19640)
                     /gene="traC"
                     /locus_tag="ACHW4Z_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR8881514.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19766..20113)
                     /locus_tag="ACHW4Z_001843"
     CDS             complement(19766..20113)
                     /locus_tag="ACHW4Z_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881515.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20141..20359)
                     /locus_tag="ACHW4Z_001844"
     CDS             complement(20141..20359)
                     /locus_tag="ACHW4Z_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881516.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20458..20931)
                     /locus_tag="ACHW4Z_001845"
     CDS             complement(20458..20931)
                     /locus_tag="ACHW4Z_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881517.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20924..21337)
                     /locus_tag="ACHW4Z_001846"
     CDS             complement(20924..21337)
                     /locus_tag="ACHW4Z_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881518.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21330..21551)
                     /gene="traR"
                     /locus_tag="ACHW4Z_001847"
     CDS             complement(21330..21551)
                     /gene="traR"
                     /locus_tag="ACHW4Z_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR8881519.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21686..22201)
                     /gene="traV"
                     /locus_tag="ACHW4Z_001848"
     CDS             complement(21686..22201)
                     /gene="traV"
                     /locus_tag="ACHW4Z_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR8881520.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22198..22518)
                     /locus_tag="ACHW4Z_001849"
     CDS             complement(22198..22518)
                     /locus_tag="ACHW4Z_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR8881521.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(22505..23095)
                     /gene="traP"
                     /locus_tag="ACHW4Z_001850"
     CDS             complement(22505..23095)
                     /gene="traP"
                     /locus_tag="ACHW4Z_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR8881522.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23085..24512)
                     /gene="traB"
                     /locus_tag="ACHW4Z_001851"
     CDS             complement(23085..24512)
                     /gene="traB"
                     /locus_tag="ACHW4Z_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR8881523.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24512..25240)
                     /gene="traK"
                     /locus_tag="ACHW4Z_001852"
     CDS             complement(24512..25240)
                     /gene="traK"
                     /locus_tag="ACHW4Z_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR8881524.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25227..25793)
                     /gene="traE"
                     /locus_tag="ACHW4Z_001853"
     CDS             complement(25227..25793)
                     /gene="traE"
                     /locus_tag="ACHW4Z_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR8881525.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(25815..26126)
                     /gene="traL"
                     /locus_tag="ACHW4Z_001854"
     CDS             complement(25815..26126)
                     /gene="traL"
                     /locus_tag="ACHW4Z_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR8881526.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(26141..26500)
                     /gene="traA"
                     /locus_tag="ACHW4Z_001855"
     CDS             complement(26141..26500)
                     /gene="traA"
                     /locus_tag="ACHW4Z_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR8881527.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(26534..26761)
                     /gene="traY"
                     /locus_tag="ACHW4Z_001856"
     CDS             complement(26534..26761)
                     /gene="traY"
                     /locus_tag="ACHW4Z_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR8881528.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(26881..27528)
                     /locus_tag="ACHW4Z_001857"
     CDS             complement(26881..27528)
                     /locus_tag="ACHW4Z_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR8881529.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(27720..28103)
                     /gene="traM"
                     /locus_tag="ACHW4Z_001858"
     CDS             complement(27720..28103)
                     /gene="traM"
                     /locus_tag="ACHW4Z_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR8881530.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            28436..29026
                     /locus_tag="ACHW4Z_001859"
     CDS             28436..29026
                     /locus_tag="ACHW4Z_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR8881531.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(29323..30144)
                     /locus_tag="ACHW4Z_001860"
     CDS             complement(29323..30144)
                     /locus_tag="ACHW4Z_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8881532.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(30255..30551)
                     /locus_tag="ACHW4Z_001861"
     CDS             complement(30255..30551)
                     /locus_tag="ACHW4Z_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881533.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(30575..>30665)
                     /locus_tag="ACHW4Z_001862"
     CDS             complement(30575..>30665)
                     /locus_tag="ACHW4Z_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR8881534.1"
                     /translation="VMVNTGGNKRQVPAKRQNRGSRNSTDDGG"
BASE COUNT         7774 a         8119 c         6906 g         7866 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
    16561 aggtgccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
    16621 ggcatctcat ggttccgctc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
    16681 atccggagcc ccctgtacca cggcaggcga caccagaatg accgcgtggt gtttctgctg
    16741 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
    16801 tgcttctgac agttgtttct gactggcgct gtcaaaaaat gcatccacag tctgtttcat
    16861 gttgaaggcg gcagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
    16921 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcggggggc
    16981 tgtaacatca gtcgtttttt gcgttgtggt catgcaacac tcctgtattt ctcatgttct
    17041 tccagccagg cttccagcga agccatctcc ggccctgact tcttccacgc cagctgccac
    17101 accgcctcat gaatactcag gccctccctg cgtttctgct gcacaaactc aaaatccggg
    17161 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
    17221 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaacttg
    17281 ccaatcatgt cgcgctgcag tggctggaac tgatccggga ataactggtt atatttcgcg
    17341 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcacgg
    17401 gccgcactgg aggccttgtc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
    17461 gcaccggtat ggcggcgaca ggtgcggtaa cctttttgaa taaactcacc gaccttgcga
    17521 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gagagtacgc
    17581 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
    17641 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
    17701 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
    17761 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
    17821 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
    17881 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
    17941 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
    18001 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
    18061 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
    18121 gagtccagca cgctgcggat aagtggctgt atcagtccgg ttttaccggc cccggaggtg
    18181 ccacagaccg ccatgttgta gttggtgtta ttcatcccct taaagaagat atcgataaac
    18241 gccagctggt tacggtaggt gggcgccagc agacctgccg gtgtcagggg gttatccgcc
    18301 actaacggca tcaggttggc cacattaaag ctctctgcgc gctgaaccac accggcctct
    18361 ttcagctgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
    18421 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
    18481 tcctgctcca cttccagtgc cgtttcatta ttgtccttgc agaaggctgt gatgttgagg
    18541 aagtaggaca cgacagaaga ctggccggag cccagccgct ggcgcagttc cccccactcc
    18601 ttcgcctctt tctccacgga cggaaaccat ttggcatatg aggtcttcga cttcttctcc
    18661 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
    18721 gtcagcgtca ggatgaacgg gcaggaaatg gacatttccg ggttcagcag attgctgtag
    18781 ttgtcggcca tgttccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
    18841 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
    18901 acattcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
    18961 tgacgtcttt tcgggtacag agagtccgga ttatggttaa tcatctcccc gacaatctcg
    19021 atgaaagcct gtgcatccac tgtctgtgtg gtgatttttg cgccgtgaaa agaggcacgg
    19081 atgattttca ccagattttc catttccaga atgtcagccc ggcttttttt cttcgaggga
    19141 gaacagtacg agataaacac ccggtaatga cgcagggtca ggggcagatt catcccctcc
    19201 ggcagcggga actgtgtcgc tgccgctttc atataatagg cacgggtaat ggcgttaaac
    19261 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat cctgtcacca
    19321 accagctgac tggacatcag atggatacac aacggaatac cgcgcggcag tttggtgcgc
    19381 agcatgtgat cgagtgcctc cacaatggac ttattcgccc cattgatggg aatggcttcc
    19441 agcataaagc ccatcgtggt gtcattcatg aacagaccgg attcctggtt gtaatcacgg
    19501 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
    19561 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
    19621 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
    19681 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
    19741 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
    19801 cctgaatttg tttttcgcga atcttattgc gctcattttc tcttacccgt ttcacctctg
    19861 ccatttcgtt tctttttctg cattctgatg cgattttatt tgccttacca agtgttaaag
    19921 gttcagactg ataattaagt gtctcctgaa atgcttctgc tgcacaggga gtttcctgta
    19981 aaatctctgt cagttgttct ggtgttgcag gttgttgcgc aaggtaaacg atgtaaaaag
    20041 gaatacatac aatcatagcc ataacgccaa caaatataaa agccagaaca gattttaaat
    20101 atatcgactt catttcattt tccttcgaag cagtaagcac tcattgccca ccaccattta
    20161 gtgtttccat cacgctgttg aaaggttcat ttgttttaac ttcaatacac tcccctgtat
    20221 ttccaaagcc cctgccgcca tgtgcttcga ttgtccgaag aacctgcacg catacgtccg
    20281 tatttggcat gggtgtaata ttccagcttc cgccaccgtt aatatttttt atcagcacaa
    20341 gaaaaataaa ggtatgcatt ctttattatc tccgcttgta tattaatcac tgaatatccc
    20401 ttcagttaac ttatgaggtc ataaaacact gcagattttc attcagattt actctcttca
    20461 gcaaacttta ctctcttcag caaacttccg gatggtatcc tgttcacgct gacgggtggc
    20521 agactcctgc attgcatcca ctttctcctg ccagagacgc cggatatcct tttcatcccg
    20581 accagtcagt tttttaccgg acagcagacg gttaagcagt tcctgtctcg catccggtac
    20641 gtcttcagtc agacgcataa gctgaagcag gctctgacgg ctgagaacca tgagtgattc
    20701 cggcaggagg gtgaagaaga aaataaaaaa gcataccgca ccacagcagg caaggaccgc
    20761 aaacaggatt ttcagatata taccatcaac gccgaacgca gcaataatac agggaatcca
    20821 tgcaatacac gcacaatacg ccaggatgtt atctattcgg ttatgtctgc gatatatctg
    20881 ttgatgttca tccagaatag aatctatttt ttcagcttct gatttatgca tagtgtttcc
    20941 tctgcgtttc ctgccagaga cgccggatat cctgttcatc ccgaccagtc agttttttac
    21001 cggacaacaa acggttaagc agttcctgtc tcacatctgg tacgtcttca gtcagacgca
    21061 taagccggag taattcctga cgactgagta ccatgcgtga ttcctccggt acgctgtaat
    21121 aattaaaaat acgactgaat accccacaac agcaggcaac ggacaggcac agggatttca
    21181 gatacaggcc agaaacacag aatgtggcaa caccacaaag aatagctata actaaaaatc
    21241 ccttacccag tgccatacac aaagaaccct gttttctgga tatccgctca tgctcatcca
    21301 gaataaaatc tattttttca ttttctgatt tatgcatagt gtttcctctg cctttcctga
    21361 tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg ggcttccgga
    21421 ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt tatttcctgg
    21481 cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc ttcatcggct
    21541 tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc atcccttttc
    21601 acgcacaggg cactcgtttt acaggcggat tttgaataca gaaagcccgc cggcattgcc
    21661 ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac gcagacggtt
    21721 taataacaaa aaacacgctg gatggctgat gataaacatc ctggttatca atataaggcg
    21781 ctatccataa tgctgccgtc tgctcacccg ttcttagcgg acgaggaggt gttaccggcg
    21841 taactgaact gaccggaaca accgttttca cttcccgggc agcggtaaac agcgggcgtg
    21901 gtgccagcag tttctgctcc ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg
    21961 tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg
    22021 atgccgcaac cggctttgct tctgaggact gctccagttt tttggccttc tcattggcct
    22081 gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc
    22141 ctgcgcagcc ggaaagtaat aaggttccca gcagaggaat aaataaagaa atctgtttca
    22201 ttgcccttct ccttttttat tactccccac aacagcagaa attaactctg ctgtcagatt
    22261 tgttttccct gcacagggat gtccggagac cactgtattt cccggagaag aaaataagct
    22321 catcgacatt cccttcttca tcacaccgac agcagccata ttaaatttgt gcggcaatga
    22381 ttcagaactg aacaccttcg gagcaggaag aactattttt gtggtgcagt tactgagaac
    22441 agcatgcata aaatcatctg atacgttttt atccggcaca acgcgggctg taataacatt
    22501 aatattacgc atgttcattt ttatctccgt tgcttctgtt tgttcggtca tcagggaaat
    22561 aaacatggca tttcctgatt tttcccggca gggaagcgtc ttccgtataa acgtccttca
    22621 gatattcatc tgcggttacc acccggatac atttcatttt tccgggcagc accggatatt
    22681 tcccggtgat cgctgccagc gagacataca tcagtgccag tgttgcccat aatgcggtga
    22741 ccagttgcat cagtgaccgg tcagtctccc tgacataatc tgcagcatca gccgcgcggt
    22801 aatggcagat atcctgtatt tctgatctgc ctgaagaatc agaggaagaa aaccagcatt
    22861 cactgaccgg gaactgtccg gcaggtgctg tttgtcttac gaagttaatc tccttaacca
    22921 gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa cagagccatc agggccattg
    22981 tggccagtgg cagaatgacg gtgtacttca gacaccagaa cagtcctcgc agaacccggg
    23041 cgacaacata ccgggcggca tggcacgcct gacgggacga catattattt gccatcgttg
    23101 cctcctccct ggctgccgga cataaactgc tgcggattca ggttgcccag ctgcttcagc
    23161 atctggtcag tattaaagcc gttaagatga ctttcagctg atggcggaac aggaaccgga
    23221 ctttccggat tatcttcttc cgctctgttc tgcgtgcgct ccagcgccat ctcttccacg
    23281 gttttcagct ggaagccgtc ctggaacacc acggtcactt cgttgcccgc accaatcggt
    23341 atcaccgggt gatactgttc ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct
    23401 ttcgatgtgc cgccaccgat acccattttc aggacatcac cagccccgta agcggctgtg
    23461 gcacccagcc cgacagccgg ctgggaggcg cgctccattc cctgaccgat accgtcaaca
    23521 aatcccgcgc cccatgccca gccgaggatt ttgccgttac gcatcaccac ttcgcccttg
    23581 ataccgtttt taccccggaa gctgacatgc cccttaatcg gcatatcaat agttttgccg
    23641 tccttcaggc aactgatatt gcgggtgcgt acaatggcac gctcactgga cacatccccc
    23701 caggcttcca gccccacaaa acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc
    23761 accaggccgg tgatacgcaa ctgcatcggc accgtggatt cattaccggt gactgaggca
    23821 ttggcatccg ccccttcaat cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc
    23881 agcgatggtc cctgttttcc ctcattacgg gtaaacacct tacgctgtat ccggttaggc
    23941 accggcacag actggtacgt cacctgtggt ggtggcgtga caccattccc gggataaaac
    24001 gccgtcggcg gtggaacagc aacgctgccc tgcggcggga aggatacggg ggtgtttcct
    24061 ggctgtggtt cgccttccgg cccgggcggt gaggcaggca tctgaggtac aggctcaccg
    24121 gtcgccgtga cgggattagc acccagggct tttacctgct ctgccagggc ggcattgtcc
    24181 tgtcccagct tttcaatacg acgctggtca tcaccgcgct gtttgttcag gacatccagt
    24241 tcacgacgga tttcctcata ctgcttctgc atctgcgctg ccgtcacctg catctctgtg
    24301 gtggcatgct gacgcacctt gtcatcaaag gtcgtatcca ccacacctgt catatccggc
    24361 acaggctcct gttcagccac cgcctcaccg ttaccggaca tgtccacatc agacagatac
    24421 agtaccccac caatcgcgga ggctgcaccg acaaccacaa tccccagcca caggtactgc
    24481 ttgcgtttca caatggtatt gatactggcc atcagttgcc ctccccgtca cggatgacat
    24541 acacatccat gcgcgcacct gccagtaact ggctggcagg ctggctgaac atcacggcac
    24601 gggttcccgg ctgccagaag tcactttccc ggatattcag ggcagacagc gttttatttt
    24661 ccacggcaaa gcggaccatc ttcaggtgat tccccgtcca tacggcatct gccactgaag
    24721 acagccccgc cggggcctgc agggtttcct ttgtcacagg aacctgatac cagcctgcag
    24781 gtaattttcc gccacggaca gcctggctga tggtcacaag cagggattcg taaggcgtgg
    24841 acgtttccca cgctccggct tcttctccgg ttccgcgcag gtcactgacc agctgaatgg
    24901 tacgccccgc gccttcacgg ggaacagcct gaatggaaag gttcagacca cgctctgttt
    24961 ccagaatgaa cgtaaagggc tttttgttga cggtggccac caccacaccg ccggaggcgg
    25021 tctgctcatt attggtcagg gcaccatcca gactgttcac ggcgataatc cggtcaccgg
    25081 gaacggtaaa cagattcggg ctggtattgc tgatggcaat actggcctga ccaccgttca
    25141 ccattggcac cacagtgggg gccagcgtac cgtttgcggc catcacgctg caggaaaaaa
    25201 acaggccgcc gaatattatt gccgtattat tttttctcat cgtctgtttc cccgaaatta
    25261 tccagccagg tcaccccgtt ttcccgcttc agaataagga tgtaatgttt gatatcagta
    25321 aaaggttcgg agttaccaat ccatgttttc cgaattccac ggatttccac acgaccatac
    25381 tgaggccaga cacggacact ggtctggaaa aaagcagaat tcacgttatc ttttttaatg
    25441 agtttcgctt cttcagccag aataactttc atctggttct gtgcacccgg gcggatatat
    25501 tgcagaagcg cctgatgtga agcatcgacg atttccggtg agacattcag acggagggca
    25561 ataaatgaca gcgccatctg ctgcaaataa gaggcatcgg cactgttctg tgacacggca
    25621 aagggcacat taaatcccat tggggtcacg gcagtgcgtt gctcattctg aagacggtag
    25681 ttattcaccc cctgaatgag gttaacagag aggctgagaa caataagcac tgacataaat
    25741 ataaaggcga tggccattac acgactggta cttaaacggg caccgtgttc catataacaa
    25801 tcctggtatc agttctattt aatccactgc cggaaacacg aatcgggaac attatgaaaa
    25861 ataccgcgca gcagggctgt tggcatatac cagtaaatca ggtcacgtaa ccaggaactg
    25921 ccccgccctt ttttcagttt tttaatcccg aaataaacca gaaccgctgc accaataccg
    25981 aacagatatt tcgatgttgt gataccccag ccaacacaga ttgctgcggg gatcagttca
    26041 tccagtggca ggccaaacca gcggctctgg ttggtcagtg tttccgggaa acgatatttc
    26101 ttaagtttat tctcgtctcc cgacatcgtt ttatttcctg tcagaggcca acgacgctca
    26161 tacccacagc aataaataca gagatgatgg caaagccgac caggaacttg acgtttttgg
    26221 tcatcatgta catgaccgca ccgaccagca cctcagccag aacaacccat ttaacgatac
    26281 tggagtcctt accgaaggtc gccttaaccg tatcgttacc tttagccatc aggtcgcctt
    26341 taccagcagc catagctaac tcagggaaca acatcatcag aacagcaacc gggatcactg
    26401 cacgaaccag gcgaagcata ttcagacgag tgaatttaga aaaaaacgat ttattttcaa
    26461 cgggcgcaga agcaccctga acacttaaaa cagtattcat atgattcctc tgattaatat
    26521 tgacagatga aaactaaagt tctttaaatg ttgactcgtt ctcttcgatc acctccctga
    26581 atatttcttc attataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg
    26641 cttcgtttgt tttcgttctt ccactacgct ctctggctcc aagtaataat ttattcgttt
    26701 cctcatcaag ggtaagtaaa acttttctac ttggcgcaga tcttaaagct cctttaatca
    26761 ccctccctcc cataaataaa agagatttag tcaaaatatt cacataagac gcacacaaaa
    26821 ataaacaaca aaatagtaaa ttacgttaac aaaaaccggc acttttagtg ccgggaatgc
    26881 ttacatttct ttacagtgaa ggctgttaat attactagcg aagtattgat atagataaaa
    26941 taacacatca gagattatag catatcgcaa ccactcatcc ctgtctgaga aaccatagtg
    27001 ttttttaaaa ttcataaata tttttttaga ttggtcaaca gagatatcca ttctctgaga
    27061 agaatactca tgactataac ccaaaagata cggtattatc ttattaaagc gatatttgtc
    27121 aggttcacaa ccaaactcct tgccctttat aaaaaagcca ctaactacgt atctttcaaa
    27181 gaatctccag acaaacaaat tcaagttatt cacttccgta ctttcaacca ccacatccca
    27241 tgatacatca ttcaatatta caccattaat aatactcact ccattcgagg acgagaaagc
    27301 atcaacttca gcagtatgaa ataaaatttg cgtatcaata tcaataccag aaaaccatat
    27361 ttcagaatca ctattctgac actttattaa gtgtaaaaaa acagagtttg agtctatgaa
    27421 ttttcctgac tctgtgcgga cacagactgg atattttgcg gataaaataa tttccggcag
    27481 gcctgattga cgtatgagtg ctctttctct agtatccatc gcacacatag gaacctcctc
    27541 aaaggattct atggacagtc gatgcaggga gggacaagct ccctgcatcg gcgattttca
    27601 agttcaaatt ttaacgtggc attaaccacg taattaatat actaattatt ctgattattg
    27661 tcatcagttt gaaccattaa aaagtctgat atttgaacaa agtcaaaatt tatttccctt
    27721 tattcatcat cattttttgg aaagaacctt tccatctcag ccgacacctt ctctctgata
    27781 tcttcaacca tattggcata ttcaaacttt ggatttcctg agacatgagg actgagagac
    27841 tcaataccca aaattttcgc tactgatgac tgtgttttta caacgcattc aagaagtagt
    27901 ttattaaact cggcctgatt aaatgcagac tctttgcgct ccatctgagc ctcatataca
    27961 cgaagaccca gttcaagaag cattgaagcc gttgctgaaa aactgacatc tttttctctc
    28021 gccccctcct gtcgacgttt ttcaataatc gcgtttattt tttcataagc atcattgctg
    28081 atatacagat tcaccttagc cattagatat tacctttttt aaacataaaa caaagttaat
    28141 ttcgaaacac tagaattcat agtgcaaaca gggacgcacc gctagcagcg cccctgacgg
    28201 tatcctataa aaaaacacac cgcgccgcta gcagcacccc taatataaaa taatgttttt
    28261 tataaaaata gtcagtacca cccctacaaa gcggtgtcgg cgcgttgctg tagctgcgtt
    28321 aacgacgctg ctttaaaaaa atcagattta aacaatataa atccacaaat acaactcaat
    28381 gatattaaag ataaatcagc aaaaacttgt ttttgcgtag tgtgtggtgc ttttggtggt
    28441 gagaaccacc aacctgttga gcctttttgt ggagtgggtt aaattattta cggataaagt
    28501 caccagaggt ggaaaaatga aaaaatggat gttagcaatc tgcctgatgt ttataaatgg
    28561 gatctgcgaa gccgccgatt gctttgatct tgcaggtcgg gattacaaaa tagacccgga
    28621 tttactgaga gcgatatcat ggaaagaatc ccgttaccgg gttaatgcca tcggtattaa
    28681 tccggtaacg ggatatggca gcggactgat gcaggtagat tcccagcatt ttaacgaact
    28741 ggcccgctat ggaattaagc cggaacatct gacaacagat ccctgtatga acatttatac
    28801 cggtgcttat tatctggcaa tagcctttaa aaaatggggc gtctcctggg aggccgttgg
    28861 tgcatacaat gccggattca ggaagaccga acgccagaac cagagacgtc ttgcctacgc
    28921 atcagaggtt taccggattt atacctggat aaagagcagt aaaggcatcc gggttccgac
    28981 cacgaagaaa tcactttccc aaattaacag tgtgcagaaa aattaatatt gtatacattg
    29041 tcaaataccg gttatgccgc gacagcggca tggaaggcca cacacaaaaa tatcatatga
    29101 gtgataatgc tgactggccg aagccccgca aagcgggatc ccatcttata tatatccggt
    29161 aactgcgcag cctcctgcca gaccattttt cgcgggcaca atatttacag actaccgaaa
    29221 agccagttga tgaaattgag gcatatcaga cgacacagca atgccgcagg atgataatat
    29281 aagtaaagcc ccgaaaaatt tttcggggct ttacttatat tatcatgact tccactccct
    29341 gaactgttcg gcaatcatcc ataatgcttt gttgattcgg atatcaccgt caatgcctgt
    29401 aatggctctt gttctggtat ttttaccgga ggcacttcgc cccgataatc caccttttat
    29461 catattctcc tgaacccgct gccaggttgt ccagagatcg ttctgtttat cctcccaacg
    29521 gcggggagta attatttgtt ctggcgtcac tggcgtttta ttttcatctt cgtacctggc
    29581 catcaatgca gctctgccaa ataaatgttg ttcgtcactg ttaagatgta tctctttcat
    29641 tgtctccata ttttcagtga ctttatcaaa gacaccgagc acttcatacg ctccctcaat
    29701 aacctgcccg acaatatcac ctttatgtgg aacgcggatt tcgccaaaat tattcccaca
    29761 caccaggcca tttgtgcaga caaaacgaaa aattcctggg atcatctgat aactggatga
    29821 accatcatgt gaattaagca ggataatttc aggaacttct ttcccgttaa tatgcccttc
    29881 cctgcgaaga cgtaacatat gtttactgta ttcgcggcgt cccaaatcac gaacccgact
    29941 ctgacaggca aagaatggct ggaaaccttc atcacgtaac ttattgatga tattgatggt
    30001 tgggatatat gtataacgct cactccggga ttcatgttta tcaccagaaa agacagatgg
    30061 aacaaactgc attaattcgt catccgttaa cgggcgctca cggcgaatag aattatatcg
    30121 accaaaacgg gaagctaatc gcataacagt aactccttta tgaaggcata aaattaataa
    30181 aagttttttc gcttaatatc atcccccctc ggttatcgtt aacaaaaaga caacaattcc
    30241 gaggggaagg accatcaaat caggtcagtt cgggactgac caacctgccg agtgaaaagc
    30301 agtaacagcg cgatcaactc atcagcgttt tttctgacaa gaggagtatg attctccagg
    30361 ctatccagta agtcatgcag ctcattattg acctggtgga gcaccttaaa gagtgcacca
    30421 gaatcccggc agctctgata tgcaagattt gcctgaacag cacgattctg catgatcccg
    30481 atttgtgcga ttgcgtcttt ctgctgatcg cctgttggct gataagtggt gttaaaagaa
    30541 aatgcgctca taattcctcc tgtatctgat gttttcagcc cccatcatct gttgagtttc
    30601 gggagccgcg gttttgacgt tttgcgggga cctgcctttt gttccctccg gtattcacca
    30661 tcacc
//
LOCUS       DAZSNG010000038        30296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZSNG010000038 DAZSNG010000000
VERSION     DAZSNG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.039"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="ACHW4Z_001863"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="ACHW4Z_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR8881535.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACHW4Z_001864"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACHW4Z_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR8881536.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACHW4Z_001865"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACHW4Z_001865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR8881537.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACHW4Z_001866"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACHW4Z_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACHW4Z_001867"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACHW4Z_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR8881538.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACHW4Z_001868"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACHW4Z_001868"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR8881539.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACHW4Z_001869"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACHW4Z_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR8881540.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="ACHW4Z_001870"
     CDS             7489..7638
                     /locus_tag="ACHW4Z_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881541.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="ACHW4Z_001871"
     CDS             7839..9422
                     /locus_tag="ACHW4Z_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8881542.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="ACHW4Z_001872"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="ACHW4Z_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR8881543.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="ACHW4Z_001873"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="ACHW4Z_001873"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR8881544.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="ACHW4Z_001874"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="ACHW4Z_001874"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR8881545.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="ACHW4Z_001875"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="ACHW4Z_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="ACHW4Z_001876"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="ACHW4Z_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="ACHW4Z_001877"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="ACHW4Z_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="ACHW4Z_001878"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="ACHW4Z_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR8881546.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="ACHW4Z_001879"
     CDS             20752..22167
                     /locus_tag="ACHW4Z_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881547.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="ACHW4Z_001880"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="ACHW4Z_001880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR8881548.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="ACHW4Z_001881"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="ACHW4Z_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR8881549.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="ACHW4Z_001882"
     CDS             24477..24809
                     /locus_tag="ACHW4Z_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR8881550.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="ACHW4Z_001883"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="ACHW4Z_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR8881551.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /gene="yegR"
                     /locus_tag="ACHW4Z_001884"
     CDS             complement(26659..26976)
                     /gene="yegR"
                     /locus_tag="ACHW4Z_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR8881552.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="ACHW4Z_001885"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="ACHW4Z_001885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR8881553.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="ACHW4Z_001886"
     CDS             complement(28363..29136)
                     /locus_tag="ACHW4Z_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8881554.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="ACHW4Z_001887"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="ACHW4Z_001887"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8881555.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7010 a         8049 c         7828 g         7409 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagcta
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgatt
//
LOCUS       DAZSNG010000039        29468 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZSNG010000039 DAZSNG010000000
VERSION     DAZSNG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.040"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..215)
                     /locus_tag="ACHW4Z_001888"
     CDS             complement(<1..215)
                     /locus_tag="ACHW4Z_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR8881556.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
     gene            525..2297
                     /gene="aspS"
                     /locus_tag="ACHW4Z_001889"
     CDS             525..2297
                     /gene="aspS"
                     /locus_tag="ACHW4Z_001889"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR8881557.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2415..2867
                     /gene="nudB"
                     /locus_tag="ACHW4Z_001890"
     CDS             2415..2867
                     /gene="nudB"
                     /locus_tag="ACHW4Z_001890"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR8881558.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2896..3636
                     /locus_tag="ACHW4Z_001891"
     CDS             2896..3636
                     /locus_tag="ACHW4Z_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8881559.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3671..4192
                     /gene="ruvC"
                     /locus_tag="ACHW4Z_001892"
     CDS             3671..4192
                     /gene="ruvC"
                     /locus_tag="ACHW4Z_001892"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR8881560.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4194..4796)
                     /locus_tag="ACHW4Z_001893"
     CDS             complement(4194..4796)
                     /locus_tag="ACHW4Z_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR8881561.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4866..4931
                     /gene="yobI"
                     /locus_tag="ACHW4Z_001894"
     CDS             4866..4931
                     /gene="yobI"
                     /locus_tag="ACHW4Z_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR8881562.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5070..5681
                     /gene="ruvA"
                     /locus_tag="ACHW4Z_001895"
     CDS             5070..5681
                     /gene="ruvA"
                     /locus_tag="ACHW4Z_001895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR8881563.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5690..6700
                     /gene="ruvB"
                     /locus_tag="ACHW4Z_001896"
     CDS             5690..6700
                     /gene="ruvB"
                     /locus_tag="ACHW4Z_001896"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR8881564.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACHW4Z_001897"
     CDS             complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACHW4Z_001897"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR8881565.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACHW4Z_001898"
     CDS             complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACHW4Z_001898"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR8881566.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8463..9395
                     /gene="znuA"
                     /locus_tag="ACHW4Z_001899"
     CDS             8463..9395
                     /gene="znuA"
                     /locus_tag="ACHW4Z_001899"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR8881567.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9411..10733
                     /gene="mepM"
                     /locus_tag="ACHW4Z_001900"
     CDS             9411..10733
                     /gene="mepM"
                     /locus_tag="ACHW4Z_001900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR8881568.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10853..11824
                     /gene="lpxM"
                     /locus_tag="ACHW4Z_001901"
                     /gene_synonym="msbB"
     CDS             10853..11824
                     /gene="lpxM"
                     /locus_tag="ACHW4Z_001901"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8881569.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACHW4Z_001902"
     CDS             complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACHW4Z_001902"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR8881570.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACHW4Z_001903"
                     /gene_synonym="yebK"
     CDS             complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACHW4Z_001903"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR8881571.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14731..16206
                     /gene="zwf"
                     /locus_tag="ACHW4Z_001904"
     CDS             14731..16206
                     /gene="zwf"
                     /locus_tag="ACHW4Z_001904"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR8881572.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16441..18252
                     /gene="edd"
                     /locus_tag="ACHW4Z_001905"
     CDS             16441..18252
                     /gene="edd"
                     /locus_tag="ACHW4Z_001905"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR8881573.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18289..18930
                     /gene="kdgA"
                     /locus_tag="ACHW4Z_001906"
                     /gene_synonym="eda"
     CDS             18289..18930
                     /gene="kdgA"
                     /locus_tag="ACHW4Z_001906"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR8881574.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACHW4Z_001907"
     CDS             complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACHW4Z_001907"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR8881575.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20298..20588
                     /gene="yebG"
                     /locus_tag="ACHW4Z_001908"
                     /pseudo
     CDS             20298..20588
                     /gene="yebG"
                     /locus_tag="ACHW4Z_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20655..21011
                     /gene="yebF"
                     /locus_tag="ACHW4Z_001909"
     CDS             20655..21011
                     /gene="yebF"
                     /locus_tag="ACHW4Z_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR8881576.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21338..21997
                     /locus_tag="ACHW4Z_001910"
     CDS             21338..21997
                     /locus_tag="ACHW4Z_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR8881577.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22207..24267
                     /gene="ptrB"
                     /locus_tag="ACHW4Z_001911"
     CDS             22207..24267
                     /gene="ptrB"
                     /locus_tag="ACHW4Z_001911"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR8881578.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACHW4Z_001912"
     CDS             complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACHW4Z_001912"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR8881579.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACHW4Z_001913"
                     /pseudo
     CDS             complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACHW4Z_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACHW4Z_001914"
     CDS             complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACHW4Z_001914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR8881580.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26077..26451
                     /gene="yobA"
                     /locus_tag="ACHW4Z_001915"
     CDS             26077..26451
                     /gene="yobA"
                     /locus_tag="ACHW4Z_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR8881581.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26455..27327
                     /gene="copD"
                     /locus_tag="ACHW4Z_001916"
                     /pseudo
     CDS             26455..27327
                     /gene="copD"
                     /locus_tag="ACHW4Z_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27340..27681
                     /locus_tag="ACHW4Z_001917"
     CDS             27340..27681
                     /locus_tag="ACHW4Z_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR8881582.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28074..29151)
                     /locus_tag="ACHW4Z_001918"
                     /pseudo
     CDS             complement(28074..29151)
                     /locus_tag="ACHW4Z_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(29117..29398)
                     /locus_tag="ACHW4Z_001919"
     CDS             complement(29117..29398)
                     /locus_tag="ACHW4Z_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR8881583.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7437 a         7236 c         7569 g         7226 t
ORIGIN      
        1 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac tcgcaccaga
       61 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
      121 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
      181 accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa aacaaaatcg
      241 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
      301 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
      361 gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
      421 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
      481 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
      541 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
      601 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
      661 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
      721 agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
      781 atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
      841 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
      901 acctcgacct gcgtcgcccg gaaatggcgc aacgcctgaa aacccgcgct aaaatcacca
      961 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     1021 ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta
     1081 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     1141 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     1201 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     1261 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     1321 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     1381 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     1441 tatttgcggg gccagcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     1501 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     1561 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     1621 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     1681 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacggga
     1741 tgggcgcact gctcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     1801 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     1861 caatgcacca tccgttcacc tcaccgaaag atatgactgc tgcagaactg aaagctgcac
     1921 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     1981 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     2041 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     2101 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     2161 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     2221 aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga
     2281 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     2341 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     2401 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     2461 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     2521 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     2581 aagtaaagga agaggttacc attgatgttg tcgctgaaca actgacctta attgactgtc
     2641 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     2701 cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag atcgttttca
     2761 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc actaagtcct
     2821 ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta
     2881 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     2941 gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     3001 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa
     3061 gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc
     3121 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     3181 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcac
     3241 gcattcagca aatgtggcgg taacctcggc actgacggtt ccgtagctta tctgttcagc
     3301 aaaaaaggcg tgatctcctt cgagaaaggc gacgaagaca ccatcatgga agcagctctg
     3361 gaagcaggtg ctgaagacgt tgtgacatat gatgacggcg cgattgatgt ctacactgca
     3421 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     3481 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcgccgaaa
     3541 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac
     3601 ggtgaaatct ctgatgaggt tgcagcgact ctctgatgat gcctgctaaa cagcaaaacg
     3661 gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga ccggctacgg
     3721 cgtcatccgc caggtaggta ggcaactgtc ctacctgggt agcggatgca tacgcaccaa
     3781 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     3841 ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc
     3901 agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtt gcggcggtga atcaggaatt
     3961 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta tgggtagtgc
     4021 cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     4081 ggcggatgcc gccgatgcgc tggcgattgc catcacccac tgccacgtta gtcagaatgc
     4141 gatgcagatg agcgaatcgc ggctgaacct gacgagaggg cgactgcgtt aagttatacc
     4201 gcctcggtca gttccggctg aggcgtttca actccctccg cgtgaatcaa caccaaaccc
     4261 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     4321 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttgtccaaac
     4381 gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa gacaaatttt
     4441 gaacagaact ggcgcgaaga ttcgtattta aaacccgtgt ggtaaagttt gcgtagccgg
     4501 gagggaacct gttcaacaat tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc
     4561 tttatagcat agcgttgatt agacgaacgt ttaataaaac gagatagcgt ggtgatggtt
     4621 gaaaggggaa cccggctttc tgcaaccagg aagtcttcac cgttatgacc gataatgatc
     4681 ccgacgtgat tactccagca atttgatgca gctgatattt gaccaaataa ggcagcacct
     4741 atacatgtaa agacgatatc tccaatttca tattcagcag ggtaattaat attcacgttg
     4801 tcattccatt gaaatagata caaatatacg taatagcata aaaaaattat ttttcaggag
     4861 tcgcaatgta tatttttatt acgcatttct tcactgaata tgtaatattc aaatatttgc
     4921 ttccaatata acctgttgaa taaattatac tgtgccattt ttcagatcat cgggacacct
     4981 cgcaagtttt cttcatcctt cgctggatat ctatccagca tttttttatc atacagcatt
     5041 atctttgatt cattacgcag gagcgtcatg tgataggcag actcagaggc atcatcattg
     5101 aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt aggctatgaa gtgcatatgc
     5161 cgatgacctg tttttatgaa ctccctgaag cgggtcagga agcgatcgtt ttcacccact
     5221 ttgtggtgcg tgaagacgcg caactgctgt acggttttaa caataaacaa gaacgcacgt
     5281 tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa gttggccctg gcgatcctct
     5341 ccggaatgtc agcgcagcag ttcgttaatg ccgttgagcg tgaagaagtg ggggcactgg
     5401 tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt gattgttgaa atgaaagacc
     5461 gatttaaagg tttgcatggc gatctcttta cgccagccgc cgacctggta ctcacgtctc
     5521 ctgctagccc ggcgaccgac gatgctgaac aagaagcagt tgccgcgctg gtggcgctag
     5581 gctataaacc acaagaagca agccgcatgg tgagcaaaat cgctcgccct gacaccagca
     5641 gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg aggtaaagga tgattgaagc
     5701 agaccgtctg atttctgccg gtaccacttt gccggaagat gtagcagatc gcgccattcg
     5761 ccctaaatta ctggaagagt atgttggtca gccgcaggtt cgttcacaga tggagatttt
     5821 catcaaagca gcgaaactgc gcggcgatgc cctcgatcat ttgttgattt ttggtcctcc
     5881 ggggttgggt aaaactacgc ttgccaacat tgtcgccaat gaaatgggcg ttaatttacg
     5941 cacgacttct ggtccggtgc tggaaaaggc gggcgatttg gctgcgatgc tcactaacct
     6001 tgaaccgcat gacgtgctgt ttattgatga gatccaccgt ttatcgccag ttgttgaaga
     6061 agtgctgtac ccggcaatgg aagactacca actggatatc atgattggtg aaggtccggc
     6121 ggcacgctcc attaaaattg atttgccgcc gtttaccctg attggcgcaa ccacgcgcgc
     6181 aggttcgctg acatcaccgt tgcgcgaccg ttttggtatt gtgcaacgtc tggagtttta
     6241 tcaggtgccg gatctgcaat atatcgtcag tcgcagtgca cgctttatgg ggcttgagat
     6301 gagtgatgac ggtgcgcagg aagtagctcg tcgcgctcgc ggtacgccac gtattgccaa
     6361 ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag cacgatggca ccatctcggc
     6421 agatatcgct gctcaggcgc tggatatgtt gaatgtcgat gctgaaggtt tcgattatat
     6481 ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt ggtgggccgg taggtctgga
     6541 taacctggcg gcagccattg gcgaagaacg tgaaaccatt gaagatgtac tggaacctta
     6601 tttgattcag caaggctttt tgcagcgtac accgcgcggg cgtatggcga cgacgcgggc
     6661 gtggaatcac tttggcataa cgccgccaga aatgccgtaa gtcggattgg tcgctcgcgc
     6721 cgcatccgac actggcagtg tgtctgatgc gacactgttc gcgtcttatc aggccagcca
     6781 gagatagcga accgtaggtc ggataaggcg ctcgcgccgc atccgacaaa tgtgttcagc
     6841 gatagattag ctggcctgct ttttcatcat actgataata aataacagtg ccgcacatag
     6901 caccaccgac ggacccgccg gcgtatcgta aaacgcggaa aaggttaaac cgccagttac
     6961 tgccaccatc cccaccaaaa cagcgacacc agccatctgt tccggcgtac gggcaaagcg
     7021 acgcgcagta gctgcaggaa taatcagcaa cgaagtaata atcaacgcac cgacaaattt
     7081 catcgctaca ccaatcgtca atgccgtcac cagcatcaac aacaatttca cttgctgtaa
     7141 tttcacacca tcaacaaacg ccagatccgg gctaatcgtc atcgacagca aattgcgcca
     7201 ttgccagaac aaaatagcca ccacgatgac cacgccaatc gcaatagaga tgagatcttc
     7261 tggcgtcact gccagtaaat caccgaacag gtaagccatc aaatcaacac gaatgttaga
     7321 catcagacta acgaccacta ggcccagcga cagggcactg tgcgccataa tccctaataa
     7381 cgtgtcgatc gccagctgtg gacgcttctc cagccatacc agaccgcccg ccagcagcag
     7441 cgtaacggca atcaccgcat agaatggatt cacgtccagc aacaaaccaa acgcgacacc
     7501 aagtaatgag gcatgagcca gcgtatcacc gaaataagac atacgacgcc agactacaaa
     7561 cgaacccagc ggacccgcgg cacaggcgag catgatcccg gctaaccaac cgggaaataa
     7621 taattcaatc atgagcgatc atttccccga cgcaaaacaa ttcgtccctg taaatcgtga
     7681 cgatgattat gatgatggcg atagataccc agttgttcag caccacgagg accaaacatt
     7741 gaaataaact ccggatgcag ggaaacaact tccggtgtgc cggaacaaca aatgtggtga
     7801 ttcaggcaca gcacttcatc ggtttttgcc atcaccagat gcagatcgtg agaaaccatt
     7861 aaaacgccac aatccagttc acgacgcaac tggtcaataa ggtcatataa cgccacttgg
     7921 ccatttacat ccacgccttg agtgggttca tccagcacta ataattgcgg gcaatttaac
     7981 aatgctcgcg ctaatagtac acgctgcgtt tcgccaccag agagcttttg catcggtgcg
     8041 ttaatcaggt gcccggcctg gacacgtttc agtgcaggca aaatatcttc tttatgtgta
     8101 ccagggcgta agcgtaaaaa acggtttacg gtcagtggca acgtggtgtc gagatacagc
     8161 ttctgcggta catagccgat gcgcagtttt ccgttgcgct tgataacccc ttcatcgggt
     8221 gttaccagcc cgagcactac ccgtaccagt gtcgacttac ctgcgccatt tggcccaagt
     8281 aaagtcaaaa tttttccagg tttaagttcc agcgacacat cagagaggac gcggcgttgg
     8341 ccaaaagaaa ccgagacatt ttccagggaa accagacttg tcatgttaat tttagtcttg
     8401 cagtagtcat gaaatgttat aatatcacac ttctcatatt cattacgatg attggtcgca
     8461 ttatgttaca taaaaaaacg cttcttttcg cagcattatc cgccgctctc tggggaggtg
     8521 caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa acccgttggg ttcatcgctt
     8581 ctgccattgc tgatggtgta acagaaacgg aggttttact gcctgacggc gcttcagaac
     8641 atgattattc actgcgtcca tcggatgtaa aacgcttaca gaacgcggac ttagtcgttt
     8701 gggttggccc ggagatggaa gcgtttatgc aaaaaccggt aagcaaatta ccagaagcga
     8761 agcaggtaac gattgcgcag cttgaagatg tgaaaccgct actgatgaaa agtattcacg
     8821 gcgatgatga tgatcacgac cacgcggaaa aaagtgacga agatcaccat cacggcgatt
     8881 tcaacatgca tctttggctt tccccagaga tagcgcgggc tacagcggtt gcaatccatg
     8941 gaaaattagt ggaacttatg ccgcaaagtc gagccaaact tgacgccaac ctgaaggatt
     9001 ttgaggcaca attggcctca accgaaacgc aggttggtaa cgagctcgcg ccgctcaagg
     9061 ggaaaggtta tttcgttttt cacgatgctt acggctattt cgaaaaacag ttcggactga
     9121 caccgcttgg tcattttacc gttaaccctg agattcaacc tggcgcgcag cgtttacatg
     9181 aaataagaac acagttggtt gagcaaaaag caacctgcgt ttttgctgag ccacagttca
     9241 ggccagcggt cgttgagagc gtcgcacgag ggacatccgt tcgtatggga acgttggatc
     9301 ccctggggac gaatatcaaa ctgggtaaaa caagctattc agaattcctg agtcaattag
     9361 ccaaccagta tgcgagctgc ctgaaaggag attaatgagg aagtgattac gtgcaacaga
     9421 tagcccgctc tgtcgccctg gcgtttaata atttaccgcg accacaccgc gttatgttgg
     9481 ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg gccctatgtt tatcaccgtg
     9541 atgccacgcc aattgtcaaa accattgagc tggaacagaa cgaaattcgt tcgctcttac
     9601 ctgaagccag tgagccgatt gatcaagctg cacaagaaga tgaagccatt ccccaggacg
     9661 aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga atatgttgtt tccactggcg
     9721 atacgctaag cagcattctc aatcagtatg gtattgatat gggtgatatc acccaacttg
     9781 ctgctgccga caaagaattg cgtaacctga aaatcggtca acaactctcc tggacattaa
     9841 ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc tcgtcgtgaa acccgaacct
     9901 atgaccgtac tgccgctaac ggttttaaaa tgaccagcga aatgcagcaa ggagagtggg
     9961 ttaacaatct gctgaaaggt accgtcggag ggagctttgt tgccagcgcc agaaacgccg
    10021 gtttaaccag cgccgaagtg agcgcagtga ttaaagccat gcagtggcaa atggatttcc
    10081 gcaaactgaa aaaaggcgat gaatttgcgg tattaatgtc tcgagaaatg cttgatggta
    10141 aacgtgagca aagccagctg ctgggcgtac gtttgcgttc agaaggtaaa gattattacg
    10201 caatccgcgc tgaggatggc aaattctacg atcgtaacgg tactggtctg gcgaaaggat
    10261 tcttgcgatt cccgacggcg aaacagttcc gtatctcgtc taactttaac ccgcgtcgaa
    10321 ctaatccggt gacaggtcgc gttgcaccac acagaggtgt tgatttcgcc atgccacaag
    10381 gtacgccagt gctttcagtg ggtgacggtg aagtggtggt tgccaaacgc agtggcgcag
    10441 caggttatta tgtggctatt cgtcatggtc gcagctacac cacgcgttat atgcacttgc
    10501 gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg cgaccgtatc gcgctttccg
    10561 gtaataccgg acgttcaact gggccgcatc tgcactatga agtatggata aaccagcagg
    10621 ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg gctgaccggc tccgatcgtc
    10681 gcgaattcct ggcgcaggcc aaagagattg tgccgcagct acggtttgat taattaacat
    10741 ccattcgcag ccggtacgca gtcagtaccg gcttttttta tttggtgcgg ggcaagttgt
    10801 gccgctacac tatcaccaga ttgatttttg ccttatccga aactggaaaa gcatggaaac
    10861 gaaaaaaaat aatagcgaat acattcctga gtttgataaa tcctttcgcc acccgcgcta
    10921 ctggggagca tggctgggtg tagcagcgat ggcgggtatc gctttaacgc cgccaaagtt
    10981 ccgtgatccc attctggcac ggctgggacg ttttgccgga cgactgggaa aaagctcacg
    11041 ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa cgtagtgaag ctgaacgcga
    11101 agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg atggcaatga tggctgagtt
    11161 ggcaatacgc gggccggaga aaattcagcc gcgcgttggc tggcaagggc tggagatcat
    11221 cgaagagatg cggcgtaata acgagaaagt tatctttctg gtgccgcacg gttgggccgt
    11281 cgatattcct gccatgctga tggcctcgca agggcagaaa atggcagcga tgttccataa
    11341 tcagggcaac ccggtttttg attatgtctg gaacacggtg cgtcgtcgct ttggcggtcg
    11401 tctgcatgcg agaaatgacg gtattaaacc attcatccag tcggtacgtc aggggtactg
    11461 gggatattat ttacccgatc aggatcatgg cccagagcac agcgaatttg tggatttctt
    11521 tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg atgaaagtat gccgtgcgcg
    11581 cgttgtaccg ctgtttccga tttatgatgg caagacgcat cgtctgacga ttcaggtgcg
    11641 cccaccgatg gatgatctgt tagaggcgga tgatcatacg attgagcggc ggatgaatga
    11701 agaagtcgag atttttgttg gtccgcgacc agaacaatac acctggatac taaaattgct
    11761 gaaaactcgc aaaccgggcg aaatccagcc gtataagcgc aaagatcttt atcccatcaa
    11821 ataaaaaagc ctctcgcgag gagaggcctt cgcctgatga taagttcaag tttgcttcag
    11881 aatattcgaa atctgttgaa ctatcattga actataggcc ggatgtggcg ttttcgccgc
    11941 atccggcaac gtacttactc taccgttaaa atacgcgtgg tattagtaga acccacggta
    12001 ctcatcacgt cgccttgggt gacaatcacc aggtcaccag acatcaagta acctttatcg
    12061 cgcagcagat taaccgcttc gctggcagct gctacgccgt cattagcgct atcaaagggc
    12121 accggcgtaa cgccacgata gagagcagtc aggttcagcg tacgttcatg gcgcgacatg
    12181 gcgaaaattg gtagaccaga gctgatacgg gaggtcatca gcgcggtacg acccgattcg
    12241 gtcatggtaa tgatcgccgt aacgcccttc aggtggttag ctgcgtacat tgccgacatg
    12301 gcaatagctt cttccacatt gtcgaactga acgtccagac ggtgtttaga aacgttgatg
    12361 ctcgggattt tttccgcacc caggcaaacg cgcgccatgg ctgcaacagt ttctgacgga
    12421 tactgcccag cggcagtttc tgcagacagc atcacagcgt cagtaccatc cagaacggcg
    12481 tttgctacgt ccatgacttc tgcacgcgtc ggcatcgggt tagtaatcat tgactccatc
    12541 atctgggtcg ccgtgattac cgctcggttt agctgacgcg cacgacggat caacgctttc
    12601 tgaatgccga ccagttccgg gtcgccaatt tccacaccga ggtcgccacg tgcaaccatt
    12661 accacgtcag aggcgaggat gatgtcatcc attgcatcct ggctgcaaac ggcttccgca
    12721 cgttcaacct tggcaacaat tttcgcatca catcctgcat cgcgtgccag gcgacgggca
    12781 tagttcagat cttcgccaca gcgtgggaag gagacagcca ggtaatctac gccaatcaac
    12841 gccgcagtct taatgtctgc tttgtctttt tcggtcagcg cttcagccga caaaccgccg
    12901 ccaagtttgt tgataccttt attgttggag aggggaccac cgacggtgac ttcggtgaac
    12961 actttcatgc cctgaacttc cagtactttt aactggacgc gaccatcgtc cagcagcagg
    13021 atgtcaccag gcacgacgtc agtaggcagg cctttgtagt cgataccgac tttttctttg
    13081 tcgccttcac ctttacccag gttggcgtcg agcaggaatt tatccccaat attgaggaaa
    13141 actttgcctt ctttaaaggt ggatacacgg attttgggcc cctggaggtc acccagaata
    13201 gccacatgac gccccagttt tgcggcaatc tcacgaactt tatccgcgcg cattttgtga
    13261 tcttcaggcg agccgtgaga aaagttcata cgtacaacgt tggcacccgc cgcgataact
    13321 ttttcaagat tattatcgcg atctgttgct gggcctaacg tggtaacgat ttttgttctg
    13381 cgaagccttt tggacatgta atactccgtt gactgaaaca accaggtgtt gcttgaacat
    13441 gaaatccgaa tgaaataacg ccgcgatggc gataaattaa tctcattcgg atgtcgtata
    13501 agattaggac agtgacagtc gcttttagcg atcgtcactt aaattaagta actgcttatc
    13561 aaaacgcgat tccttcagcg cttccttgac ccgcttcaag ttatctctga attttgcacc
    13621 gcgtcgcaaa gtaaatcctg tcgccagcac atctatcacg gtcagctgtg caagtcgaga
    13681 aaccatgggc atataaatgt cagtatcttc cggtacgtcg agggtaattg ccagcgttgc
    13741 ttcccgggcg agcggggtac ccgcagaggt gagggcaatc accatggcgt cgttttcgcg
    13801 tgccagctgc gccagctcga ccagattttt tgttcttcct gtgtgagaaa tcagcaccac
    13861 cacgtctccg tcgctacaat tcatacaact catgcgttgc agcacgatat catcggagta
    13921 caccaccgga acattaaaac gaaagaactt attcatcgca tcgtgggcaa cggcggcaga
    13981 agagcctaat ccgaaaaagg cgattttttt tgcctgagtg agcaagtcga cggcgcggtt
    14041 gatggcagat ttatccagtg aatgacggac atgatcgagc gttgccattg cggactcaaa
    14101 tattttcccc gtgtatgatt caacgctgtc atcttcattg acgttgcgat taacataggg
    14161 agtgccattc gccagacttt gtgccagatg aagtttaaaa tcaggaaaac cgcgcgtgtc
    14221 catgctgcga cagaaacgat tcaccgtcgg ttcgctaaca ttggcttcca gcgccagagc
    14281 agcaatactc gaatggatcg cgttatcggg cgaagccaga atgacctcgg caactttgcg
    14341 ctctgatttg ctcaaatgtt ccagctgaga ctggattttt tccagcatat tcatgatgta
    14401 aagagactca cgggtaatga cgatttccgc actgaaagaa atcgaaatgc agttttgtca
    14461 gatattacgc ctgtgtgccg tgttaatgac aaaagcagat aaaaaagttg ttattttttt
    14521 cataacatga tcagtgtcag atttttaccg aatggaaaac gatgattttt ttatcagttt
    14581 tgccgcgctt tgcgcgcttt tcccgtaatc gcacgggtgg ataagcgttt acagttttcg
    14641 caagctcgta aaagcagtac agtgcaccgt aagaaaatta caagtatacc ctggcttaag
    14701 taccgggtta gttaacttaa ggagaatgac atggcggtaa cgcaaacagc ccaggcctgt
    14761 gacctggtca ttttcggcgc gaaaggcgac cttgcgcgtc gtaaattgct gccttccctg
    14821 tatcaactgg aaaaagtcgg tcagctcaac ccggacaccc ggattatcgg cgtagggcgt
    14881 gctgactggg ataaagcggc atataccaaa gttgtccgcg aggcgctcga aactttcatg
    14941 aaagaaacca ttgatgaagg tttatgggac accctgagtg cacgtctgga tttttgtaat
    15001 ctcgatgtca atgacactgc tgcattcaac cgtctcggcg cgatgctgga tcaaaaaaat
    15061 cgtatcacta ttaactactt tgccatgccg cccagcactt ttggcgcaat ttgcaaaggg
    15121 cttggcgagg caaaactgaa tgctaaaccg gcacgcgtag tcatggagaa accgctgggg
    15181 acgtcgctgg cgacctcgca ggaaatcaat gatcaggttg gcgaatactt cgaggagtgc
    15241 caggtttacc gtatcgacca ctatcttggt aaagaaacgg tgctgaacct gttggcgctg
    15301 cgttttgcta actccctgtt tgtgaataac tgggacaatc gcaccattga tcatgttgag
    15361 attaccgtgg cagaagaagt ggggatcgaa gggcgctggg gctattttga taaagccggt
    15421 cagatgcgtg acatgattca gaaccacctg ctacaaattc tctgcatgat tgcgatgtct
    15481 ccgccatctg acctgagcgc agacagcatc cgcgatgaaa aagtgaaagt actgaagtcc
    15541 ctgcgccgca tcgaccgctc caacgtacgc gaaaaaaccg tacgcgggca atatactgcg
    15601 ggcttcgccc agggcaaaaa agtgccggga tatctggaag aagagggcgc gaacaaaagc
    15661 agcaatacag aaaccttcgt ggcgatccgc gtcgacattg ataactggcg ctgggccggt
    15721 gtgccattct acctgcgtac tggtaaacgt ctgccgacca aatgttctga agtcgtggtc
    15781 tatttcaaaa cacctgaact gaatctgttt aaagagtcgt ggcaggatct gccgcagaat
    15841 aaactgacta tccgtctgca acctgatgaa ggcgtggata tccaggtact gaataaagtt
    15901 cctggccttg accacaaaca taacctgcaa atcaccaagc tggatctgag ctattcagaa
    15961 acctttaatc agacgcatct ggcggatgcc tatgaacgtt tgctgctgga aaccatgcgt
    16021 ggtattcagg cactgtttgt acgtcgcgac gaagtggaag aagcctggaa atgggtagac
    16081 tccattactg aggcgtgggc gatggacaat gatgcgccga aaccgtatca ggccggaacc
    16141 tggggacccg ttgcctcggt ggcgatgatt acccgtgatg gtcgttcctg gaatgagttt
    16201 gagtaatatc agcgcttatc ctttatggtt attttaccgg taacatgatc ttgcacagat
    16261 tgtagaacaa tttttacact ttcaggcctc gtgcggattc acccacgagg ctttttttat
    16321 tacactgact gaaacgtttt tgccctatga gctccggtta caggcgtttc actcataaat
    16381 cctctgaatg aaacgcgttg tgaatcatcc tgctctgaca actcaatttc aggagccttt
    16441 atgaatccac aattgttacg cgtaacaaat cgaatcattg aacgttcgcg cgagactcgc
    16501 tctgcttatc tcgcccggat agaacaagcg aaaacgtcga ccgttcatcg ttcgcagttg
    16561 gcatgcggta acctggcaca cggtttcgct gcctgccagc cagaaaacaa agcctctttg
    16621 aaaagcatgt tgcgtaacaa tatcgccatc atcacctcct ataacgacat gctctccgcg
    16681 caccagcctt atgaacacta tccagaaatc attcgtaaag ccctgcatga agcgaatgcg
    16741 gttggtcagg ttgcgggcgg tgttccggcg atgtgtgatg gtgtcaccca ggggcaggat
    16801 ggaatggaat tgtcgctgct aagccgcgaa gtgatagcga tgtctgcggc ggtggggctg
    16861 tcccataaca tgtttgatgg tgctctgttc ctcggtgtgt gcgacaagat tgtcccgggt
    16921 ctgacgatgg cagccctgtc gtttggtcat ttgcctgcgg tgtttgtgcc gtctggaccg
    16981 atggcaagcg gtttgccaaa taaagaaaaa gtgcgtattc gccagcttta tgccgaaggt
    17041 aaagtggacc gcatggcctt actggagtca gaagccgcgt cttaccatgc gccgggaaca
    17101 tgtactttct acggtactgc caacaccaac cagatggtgg tggaatttat ggggatgcag
    17161 ttgccaggct cttcttttgt tcatccggat tctccgctgc gcgatgcttt gaccgctgcc
    17221 gctgcgcgtc aggttacacg catgaccggt aatggtaatg aatggatgcc gatcggtaag
    17281 atgatcgatg agaaagtggt ggtgaacggt atcgttgcac tgctggcgac cggtggttcc
    17341 actaaccaca ccatgcacct ggtggcgatg gcgcgcgcgg ccggtattca gattaactgg
    17401 gatgacttct ctgacctttc tgatgttgta ccgctgatgg cacgtctcta cccgaacggt
    17461 ccggccgata ttaaccactt ccaggcggca ggtggcgtac cggttctggt gcgtgaactg
    17521 ctcaaagcag gcctgctgca tgaagatgtc aatacggtgg caggctttgg tctgtctcgt
    17581 tatacccttg aaccatggct gaataatggt gaactggact ggcgggaagg ggcggaaaaa
    17641 tcactcgaca gcaatgtgat cgcttccttc gaacaacctt tctctcatca tggtgggaca
    17701 aaagtgttaa gcggtaacct gggccgtgcg gttatgaaaa cctctgccgt gccggttgag
    17761 aaccaggtga ttgaagcgcc agcggttgtt tttgaaagcc agcatgacgt tatgccggcc
    17821 tttgaagcgg gtttgctgga ccgcgattgt gtcgttgttg tccgtcatca ggggccaaaa
    17881 gcgaacggaa tgccagaatt acataaactc atgccgccac ttggtgtatt attggaccgg
    17941 tgtttcaaaa ttgcgttagt taccgatgga cgactctccg gcgcttcagg taaagtgccg
    18001 tcagctatcc acgtaacacc agaagcctac gatggcgggc tgctggcaaa agtgcgcgac
    18061 ggggacatca ttcgtgtgaa tggacagaca ggcgaactga cactgctggt agacgaagcg
    18121 gaactggctg ctcgcgaacc gcacattcct gacctgagcg cgtcacgcgt gggaacagga
    18181 cgtgaattat tcagcgcctt gcgtgaaaaa ctgtccggtg ccgaacaggg cgcaacctgt
    18241 atcacttttt aagacgacaa atttgtaatc aggcgagaga aaactctgat gaaaaactgg
    18301 aaaacaagtg cagaatcaat cctgaccacc ggcccggttg taccggttat cgtggtaaaa
    18361 aaactggaac acgcggtgcc gatggcaaaa gcgttggttg ctggtggggt gcgcgttctg
    18421 gaagtgactc tgcgtaccga gtgtgcagtt gacgctatcc gtgctatcgc caaagaagtg
    18481 cctgaagcga ttgtgggtgc cggtacggtg ctgaatccac agcagctggc agaagtcact
    18541 gaagcgggtg cacagttcgc aattagcccg ggtctgaccg agccgctgct gaaagctgct
    18601 accgaaggga ctattcctct gattccgggg atcagcactg tttccgaact gatgctgggt
    18661 atggactacg gtttgaaaga gttcaaattc ttcccggctg aagctaacgg cggcgtgaaa
    18721 gccctgcagg cgatcgcggg tccgttctcc caggtccgtt tctgcccgac gggtggtatt
    18781 tctccggcta actaccgtga ctacctggca ctgaaaagcg tgctgtgcat cggtggttcc
    18841 tggctggttc cggcagatgc gctggaagcg ggcgattacg accgcattac taagctggcg
    18901 cgtgaagctg tagaaggcgc taagctgtaa aagtcaaaat gcccgatcta ggatcgggca
    18961 tttttgtagc gtttttttgc cgggtttaac cctgtacttt tacctgtccg gcggcgtgct
    19021 tcgcgcgttc aatggcgtca acaacactct ctgaagtagc cagtaccacc cccagatgac
    19081 ggctgccatc aatttccggc ttaccaaata aacgaatctg caaatctgcg cctacggcat
    19141 tctgcacatt atcaaacgtg acattctgac tggtcagttg tggcagaata acggcagaag
    19201 ctgcaggacc atactgacgg atcccgccaa caggaagtcc gaggaaggca cgtacatgca
    19261 gggcaaactc tgagagatct tgagaaatta acgtcaccat cccggtatca tgtggacgag
    19321 gggagacctc actgaaaatc acctcatcac cacagacaaa cagctcgaca ccaaacagac
    19381 cataaccgcc cagtgccagc accactttac gggcaatctc ctgcgcacgt tcaagggcaa
    19441 gcgggctcat ttgctgtggt tgccaggatt cacggtagtc gccatcttcc tggcgatgac
    19501 ctactggtgc acagaaatgg acgccatcca ccgcgctgac ggttagcagg gtaatttcga
    19561 agtcaaactt aacgacgcct tcaacaatta cgcggcccgc tccggcgcga ccgccttgct
    19621 gagcgtattc ccatgcctga gcaagttgct ctgcagagcg aataaacgtc tgccccttgc
    19681 cggaagagct catcaccggt tttacaatgc agggatagcc aatggcggca accgcctcgc
    19741 ggaaaaggct tttgctatcg gcaaaacgat aagtggaagt gggcagctgc agctcttctg
    19801 ccgccaggcg acggataccc tcgcgattca tcgttaattt cgttgcgcga gcgcagggga
    19861 caacattcag tccttcctct tcaagttgga tcagcatatc ggtggcaata gcttcgatct
    19921 ccggcacgat ataatgtggt ttttccagtt caaccacacg gcgtaatgca tcaccatcaa
    19981 gcatattaat gacatgggag cgatgcgcga catgcatggc tggtgcgtcg gcatagcgat
    20041 cgacggcaat cacctctacg ccgagacgct gacactcgat tgccacttct ttacccagtt
    20101 caccggagcc taataacatc acgcgagttg ctgccggacg cagcgcagtg cctaataacg
    20161 tcataacgaa tatcccctga ttaattccgc gcgcagtata aacgaaaacg tttgcgtgtg
    20221 tctttattgc tgatgttgat ttctcaaccg aaaagaaata tactgtataa aatctcagtt
    20281 attatgagag gctgcaaatg gcggttgaag ttaaatacgt agtcattcgt gagggcgaag
    20341 agaaaatgtc gtttaccagc aaaaaggaag ccgatgctta tgacaaaatg cttgatacgg
    20401 cggatctgct tgacacctag ctgacaaatt ctccagtgca aatggaagac gagcaacgtg
    20461 aagccctttc gctatggctg gcagaacaaa aagaggtgct gagcaccatt ctgaaaaccg
    20521 gcaaattacc gtccccacag gtcgtcggtg ctgaatccga agaggaagat gcctcacacg
    20581 cggcctgaag caacttgcgc tcctgacaat ttgtaccatg ctttctcctg aatcattgtg
    20641 ctatggagaa aaacatgaaa aaaagagggg cgtttttagg gcggttgttg gtttctgcct
    20701 gcgcatcagt tttcgctgcc aataatgaaa ccagcaagtc ggtcactttc ccaaagtgtg
    20761 aaggtctgga tgctgccgga attgccgcga gcgtaaaacg tgattatcaa caaaatcgcg
    20821 tggcgcgctg ggcagatgat caaaaaattg tcggtcaggc cgatcccgtg gcctgggtca
    20881 gtttgcagga cattcagggt aaagatgata aatggtcagt accgctaacc gtgcgtggta
    20941 aaagtgccga tattcattac caggtctgcg tggactgcaa agcgggaatg gcggaatatc
    21001 agcggcgtta atggcttctg ataattcgat tgaccgttgg cggatgcggc gcgagtgcct
    21061 tgtccgtccc agaacgcacc acaatcgtag gcctgagaag acgcaacagc gtcgcatcgg
    21121 gcaatcagca tcaaacaatg ccactcaaaa cgacaaacta ctttcaccta aaataccctt
    21181 atcacctgat atacagtaaa taccttacct ggcgcgaatt tctttgaata acggcctacc
    21241 gccccgctta tttgcgcatc ttttgtctcg atatacattt tctgacatcc tcatccgcta
    21301 tgctacaggc actaaaaatg gttattgagg agcaaatatg gctaactggt taaatcaact
    21361 gcaatccctg cttgggcaaa gcagttcttc tacctcctcg tctgcggatc agggattggg
    21421 caaactgtta gtgtcaggcg cattaggcgg actggctggg ctgctggtcg caaataaatc
    21481 agcacgtaaa cttcttacga aatatggcac caacgcgtta ctggttggcg gcggagcggt
    21541 agcgggtacg gtgctgtgga ataaatacaa agataaaatt cgcgcggcgc atcaggacga
    21601 accgcagttt ggcgcgcaaa gtacgccgct ggatgagcgt acggaacgtt tgatccttgc
    21661 gctggtcttt gccgctaaaa gtgatggtca tattgatgcc aatgaacgtg cggcaatcga
    21721 ccagcaattg cgtgaagccg gcgtggaaga gcaggggcgt gtactcattg agcaggcaat
    21781 cgaacaaccg ctggatccac aacgcctggc taccggggtc cgcaatgaag aagaggcgct
    21841 ggaaatctat ttcctgagct gcgcggctat tgatattgac cattttatgg aacgcagtta
    21901 cctgaatgca ctgggtgacg cgctgaaaat cccccaggat gttcgtgaag gcattgaacg
    21961 cgatctcgaa cagcaaaaac gcactttggc ggaataattc cgctctctgg atagcctgaa
    22021 gttgactatt tcgggctatc cagaaccgca cagccatttg aacaggctga ttttctctgt
    22081 ctgcataaca ttcgatatcc gcgccattac ctgtcatcat ctaagcaatg acgaccctgt
    22141 ttcgcttgca tccccggtga gttttgccac ccttataaga tgtttccaac cagaaagaac
    22201 aataacatgc taccaaaagc cgcccgcatt ccccacgcca tgacgcttca tggcgatacg
    22261 cgcatcgata attactactg gctgcgggac gatacgcgtt ctcagccaaa agtcctggac
    22321 tacctgcaac aagaaaatag ttacggtcat cgggtgatgg cctcacaaca agccttgcag
    22381 gatcgcatct taaaggaaat catcgaccgc attccgcaaa gagaagtttc tgcgccctac
    22441 ataaaaaatg gctaccgcta tcggcatatt tatgaaccag gctgtgaata tgctatctac
    22501 cagcgtcaat cggcattcag cgaagagtgg gatgagtggg aaacattgct cgatgccaac
    22561 aagcgcgcag ctcatagtga gttttattcg atgggcggaa tggcgattac gcccgataac
    22621 accattatgg cgctggcaga agattttctt tcccgacgcc agtacggcat tcgttttcgt
    22681 aatctggaaa caggtaactg gtacccggaa ctgctggata acgttgaacc cagctttgtc
    22741 tgggcaaatg actcctggac tttctactat gttcgcaagc atccggtgac gctgctgcct
    22801 tatcaggtct ggcgtcacgc catcggtacg ccagcatcgc aagatacact gatctacgaa
    22861 gaaaaagacg atacctatta tgtcagcctg cataaaacga cgtcgaagca ctatgtcgtc
    22921 attcatctgg ccagcgccac caccagtgaa gttcgcctgc tggacgcgga aatggccgat
    22981 gccgagccgt ttgtttttct gccgcgccgc aaagatcacg aatacagcct tgatcactac
    23041 cagcatcgtt tttatctgcg ttccaatcgc cacggcaaaa actttggctt ataccgtacc
    23101 cgtatgcgtg atgagcaaca gtgggaagag ttaattccgc cacgcgaaaa catcatgctg
    23161 gaagggttta cgctgtttac cgactggctg gtggttgaag agcgtcagcg cgggttaacc
    23221 agtttgcgcc aaattaaccg caagacccgg gaagtcattg gcattgcctt tgatgatccg
    23281 gcctatgtga cctggattgc ctacaatcca gaacctgaaa ccgcgcgatt gcgttatggt
    23341 tattcttcca tgaccacacc agacactttg tttgaactgg atatggatac cggtgagcgt
    23401 cgtgtattaa aacaaacaga agtgcctggt tttgatgcgg cgaattaccg cagtgaacac
    23461 ctgtggatag tcgcccgtga tggcgtcgaa gttccggttt cgctggtcta tcatcgcaaa
    23521 cattttcgca aaggacacaa cccgttgctg gtgtatggct atggttctta cggcgcaagt
    23581 attgatgccg atttcagttt tagccgcttg agtttgttag atcgtggctt tgtctacgcc
    23641 attgtccatg ttcgcggcgg tggtgagctg gggcaacaat ggtacgaaga cggtaaattt
    23701 ctgaagaaga aaaatacgtt taatgattat cttgatgcct gcgatgcatt gttaaaactg
    23761 ggctatggct ctccttcgct ctgttatgcg atgggcggaa gtgcgggggg catgttgatg
    23821 ggcgttgcaa ttaatcaacg cccggaatta ttccacggcg ttatcgccca ggtaccgttt
    23881 gttgatgttg taacaacgat gcttgatgaa tcaattcctc ttaccactgg tgagtttgaa
    23941 gagtggggaa atccgcagga tccgcaatat tacgagtaca tgaaaagcta cagcccgtat
    24001 gacaacgtca ccgcacaggc ttatccgcat ttactggtaa cgaccggttt acacgattct
    24061 caggtgcaat attgggaacc ggcaaaatgg gtcgctaaat tgcgcgagct gaaaaccgat
    24121 gaccatcttt tattgctctg taccgacatg gactcaggcc atggcggtaa atctggccgc
    24181 tttaaatcgt acgaaggcgt agcgatggaa tatgcttttc tggtcgcgct ggcgcaggga
    24241 acattacccg ctacgcctgc ggattaagta ttttccagat aatgtttcag tgttaaacgc
    24301 agctccgggc tcatgctgtc caggttatta aataaccagc gcagatagcc cggatagcgt
    24361 tcggcaacgt cggaaaccgc tttgccacgg tatttgccaa aggtgaaggt tgtcatcaac
    24421 gacggacgtc cggtgatatc ggccatctgt tctgccgtcc agccggaggt gttcataata
    24481 tcgataagca acgcggcggt gatataacag tcatacaacg cgcggtgatg atgcaggccc
    24541 agcggtgtct gtacattgag cttgcgtgtt ttatataacg ccatattgct gtacttgagc
    24601 cctggccaca aacgacgggc cagtttcata gtgcaaatcc actcaccggg catctcaggc
    24661 agtacgcggc ggtcaaagct ggcgttgtgc gcgacatacc attcactacc gtaatagtgt
    24721 gggatcacat cttcaatcca cggtttatcg gcgaccatgg cttcggtgat gcgatgaatc
    24781 gccatcgctt gtggactaat aggacgatcg gggcgcacca ggaggctcat ggggttgacg
    24841 atttttccgt caatgacatc aacagaggca atctcaacga tccctccctg caaaccgcag
    24901 gtttctgtat cgataatgcg caacatggca tgatccaggc cgaaaaagcc tagcgtaatg
    24961 gaatgatatc gccttgccaa ccctgtgatg aacgctgacc gactaacaat aatgaaccac
    25021 gatcggcgcg aacgatgaga cgaccatatt catcccatag tgcactgtta ccacgggcat
    25081 ttgccatcag cacggcaatc gagtactgat gtgaaaaaaa ttgtaagcga cgggccgacg
    25141 ccagcaggtc gggttctccc agacattgcc ctgtcgtaaa cagtgaacag gtcgggtcca
    25201 tatccatacc ttgcggttgt tcatcgacca ccgtgatggt tctggaacga cggcccagac
    25261 atgcaccatg actctgatgg tagttcccag gcgtttttcg ccacggtgcg aacaccgcaa
    25321 ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc ggcaatgatg gtcattcgct
    25381 aggtcgttgc ggcataacaa agcgggtcta acagtgaaag atcgggtggg gcaggaaggg
    25441 cacgtcgcga ataatcacac cccagtaaag aaagcgatgg aaaaactaac agctgacatt
    25501 gttgcctggc ggcggccctc acaaactcca gatgatgagc tacctgttcg gtgagtgagg
    25561 ttttacgagg ctcatattgc gctgctgcaa ctttccagaa cgacataatg acttcctttt
    25621 caaaatgact gcaaattatg ggggcggttt agaaaatact cataatttag caggcgttat
    25681 gtaagaaagt gtaactctgt ataagtttta tttaagtttg ggctcgtaag gtaaacgtga
    25741 cagattgacc gaagccaaac ggtgggcaat aagccgctcg cgaaaccagc tgcgcaaatg
    25801 ttctggctgt tcacgttcaa ccgcttcagc gatcaccggc atattgtagc gttctttaaa
    25861 agccaccccg gccgccgcca aatcgacatt cactttatcc atttctgttt gatccagttt
    25921 agccagattc ttcagcattc ttaatctcct catcattcgc gggcaaaagt tactaagagt
    25981 gagcctggat ggcaagccgg aattgatgat cctcgactca gacattagac aaggttatgc
    26041 tctgtaaaaa agttattaca aaaaggaata gataatatgg cttcaactgc acgctccctt
    26101 cgctacgcgc tggcaatcct gaccacttcg ttggtgacac cttccgtctg ggcacatgcg
    26161 catttaacgc atcagtatcc tgcggcaaac gcgcaagtga cagctgcccc gcaggcaatc
    26221 actttaaact tctcggaagg cgttgaaaca ggattcagtg gtgcaaaaat cacggggcca
    26281 aaaaacgaaa atattaaaac attaccagcg aagcggaatg agcaggacca aaagcaactg
    26341 attgttccac tggccgattc gctgaaaccc ggaacctaca ccgttgactg gcatgttgtt
    26401 tcggtggacg ggcataaaac gaaaggacac tacaccttta gcgtgaaata aatcatgctg
    26461 gcgtttacct ggatcgcgct gcgatttatc catttcacct cgttgatgct ggtctttggc
    26521 tttgcaatgt atggtgcctg gctggccccc ttaatgattc gtcgcttgct gacgaagcgt
    26581 tctctgcgtt tacaacagca cgctgccgtc tggagtttga tcagtgccac tgcaatgctc
    26641 gccgttcagg gcgggttgat gggaacggga tggacagatg tattttcgcc aaacatttgg
    26701 caggctgttt tgcaaacgca gtttggtggc gtctggttat ggcaaattgt tctcgccctc
    26761 gtaacgttga tcgtcgccct tatgcaaccg cggaatatgc cacgtttgct gtttatgctc
    26821 actaccgcgc aatttattct gctggcggga gtggggtatg cgacgctgaa tgaaggggta
    26881 acagcgaaaa tccatcagac taatcacgcg attcacctga tttgtgcggc ggcgtggttt
    26941 gggggattac tgccggtgct ctggtgtatg caactcatca aaggccgttg gcgacactag
    27001 gctattcagg cgctgatgcg tttttcctgg tgcgggcatt ttgcggtgat cggcgtactg
    27061 gcaagcggcg tgcttaatgc attgttgatt accggatttc cccccacgct tacgacttac
    27121 tggggccaac tcttattgct caaagccatc ctggtgatga ttatggtggt cattgcgctg
    27181 gctaatcgtt atgttcttgt accgcgcatg aggcaggatg aagatcgtgc ggcaccctgg
    27241 tttgtgtgga tgactaaact cgaatgggcg ataggtgcgg tagtgctggt gatcatcagc
    27301 ctgcttgcga ctctcgaacc tttttgatgg attacgaaaa tgatgaaaaa aagtattctg
    27361 gcgtttctgt tactcaccag ttctgcagcg gcgctggcgg ctccacaggt gataaccgtg
    27421 agtcgttttg aagtgggtaa agacaaatgg gcgtttaatc gcgaagaggt aatgctgact
    27481 tgccgaccgg gtaatgcttt gtatgtcatc aacccaagta ccctcgtgca gtatccttta
    27541 aacgatatcg cacaaaagga agttgccagt gggaagacca atgcccaacc catttcggtg
    27601 attcagattg atgatcctaa caatcccggc gaaaaaatga gtctggcacc gtttatagaa
    27661 cgagctgaaa aactctgtta attacctaaa atagcctttt gatttccaat aaaaaaaccg
    27721 cctcagttct ttcaccagaa cgggcggttt ttaacattta agctgatgag caccgcgctt
    27781 tttattgacc attttgcacg caaactggaa aacctggcgt cgtcatctat tcttaaaggg
    27841 caaggcaact aagcctgcat taatgccaac ttttagcgca cggctctctc ccaagagcca
    27901 tttccctgga ccgaatacag gaatcgtatt cggtcttttt ttaattgtat ttaaaatcaa
    27961 ttagttgcaa acgtctcccc gaaattcccc gaaatttact cgaatttctg tattccggtc
    28021 ttttttggtt atatcacaac caaaatacat ttaacaatcc atttacgtta aaatcagagc
    28081 agtaagtacg ttttttctct ctcatcaaga tacatttttg ttgtcttctc cgatgtgtgg
    28141 ccaagtagac gctgagcaaa ttcttctcca catatttctt tgtacaatcg tccagccagg
    28201 cttctgattt cgtgaaaagt tggtgggttt tcactgaaca gtgatacctg ttaattttct
    28261 ggctgcgaca aatttctttg tcaggccgtc cgggtgaatg ctgccgtcag ggctgttttt
    28321 tctaatcccg gcactgatta gataatcccc cctacttacc atgcggcact gttcaactac
    28381 tgcaccaagt cgtaaaccag cgacaggaag gctgagtgac agggggatag caatcatcat
    28441 tcctgtctta atttgcctga tgtggagacg atcatcataa atatcactga accgcatatt
    28501 cgttatgtct tcgcgacgtt gtcctgttac aagggctaag tccatagcta atgggaacca
    28561 tgccggaagt tgatctgctg cctccctgat gcagttgtat gtctttagtt tcagccgttc
    28621 tcttgtaact actattttcg gtgctcttgt tggcgttact ggattttgag atatacgtcc
    28681 ttcaacaatg gcttcgcgga acatatcaga taacacagaa cgcattgatc ctgccatcgt
    28741 gtttttccct ccttcaatct acaaatcaag aaactcggcg atatggcgag tggttatttc
    28801 tgtcagtaaa acccctccta atttttcttt tattgtctcc agttgattta cccgagtttt
    28861 ataagtattt ctggacgctt ttctccttat aagaatcgtt ttgtaacgtt caatccagcc
    28921 tgccatagta aatgagtcga cccctttaag cttttcaatt agggcagcag gagagtagtt
    28981 tttgtatata taatgatttg cttcaattgc ctgcgctact gcatctcttc ttgaaatttt
    29041 acctagtgta aattcttctt tcgtcagagg gttgcgccag taatatgctt tgtctctcct
    29101 tcgatatgtt aagtttctag gcaaattggg atcgtatttt ttccgctgca tgttttaact
    29161 tctccattaa cggactgtct ctcccttgtc gcccattagg ctgatggtgt gttatatcgg
    29221 tatcaacctt atttgggttg atatagaaag cctccggaac caccctgtaa ctcctcccgt
    29281 gtagttcagg tgcaggataa atgtttccat tccttgccca tcgtctcagc gttgatattg
    29341 atggtgggtt atccggatat ctgagttttc cccacgtttt gagtgtcaca agattcattg
    29401 ccatacctct cacgatatga ccgccagtaa atatacagaa tactggcggg tgtggttgat
    29461 ttttaata
//
LOCUS       DAZSNG010000040        29247 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZSNG010000040 DAZSNG010000000
VERSION     DAZSNG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29247
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.041"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..441)
                     /locus_tag="ACHW4Z_001920"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="ACHW4Z_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHW4Z_001921"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHW4Z_001921"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR8881584.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHW4Z_001922"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHW4Z_001922"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR8881585.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHW4Z_001923"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHW4Z_001923"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR8881586.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHW4Z_001924"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHW4Z_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="ACHW4Z_001925"
     CDS             5754..6257
                     /locus_tag="ACHW4Z_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR8881587.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHW4Z_001926"
     CDS             complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHW4Z_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR8881588.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHW4Z_001927"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHW4Z_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR8881589.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="ACHW4Z_001928"
     CDS             7052..7375
                     /locus_tag="ACHW4Z_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR8881590.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="ACHW4Z_001929"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="ACHW4Z_001929"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR8881591.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="ACHW4Z_001930"
     CDS             complement(8080..8319)
                     /locus_tag="ACHW4Z_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR8881592.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="ACHW4Z_001931"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="ACHW4Z_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR8881593.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="ACHW4Z_001932"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="ACHW4Z_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR8881594.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="ACHW4Z_001933"
     tRNA            complement(10643..10729)
                     /locus_tag="ACHW4Z_001933"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="ACHW4Z_001934"
     tRNA            complement(10742..10815)
                     /locus_tag="ACHW4Z_001934"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="ACHW4Z_001935"
     tRNA            complement(10870..10945)
                     /locus_tag="ACHW4Z_001935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="ACHW4Z_001936"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="ACHW4Z_001936"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR8881595.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="ACHW4Z_001937"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="ACHW4Z_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR8881596.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="ACHW4Z_001938"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="ACHW4Z_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR8881597.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14329..14400
                     /locus_tag="ACHW4Z_001939"
                     /pseudo
     CDS             14329..14400
                     /locus_tag="ACHW4Z_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            14483..14659
                     /gene="yecU"
                     /locus_tag="ACHW4Z_001940"
     CDS             14483..14659
                     /gene="yecU"
                     /locus_tag="ACHW4Z_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR8881598.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /gene="yecF"
                     /locus_tag="ACHW4Z_001941"
     CDS             14646..14870
                     /gene="yecF"
                     /locus_tag="ACHW4Z_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR8881599.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="ACHW4Z_001942"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="ACHW4Z_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="tcyN"
                     /locus_tag="ACHW4Z_001943"
     CDS             complement(15753..16505)
                     /gene="tcyN"
                     /locus_tag="ACHW4Z_001943"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR8881600.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="ACHW4Z_001944"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="ACHW4Z_001944"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR8881601.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="ACHW4Z_001945"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="ACHW4Z_001945"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR8881602.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="ACHW4Z_001946"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="ACHW4Z_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8881603.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="ACHW4Z_001947"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="ACHW4Z_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR8881604.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="ACHW4Z_001948"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="ACHW4Z_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="ACHW4Z_001949"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="ACHW4Z_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR8881605.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="ACHW4Z_001950"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="ACHW4Z_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR8881606.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="ACHW4Z_001951"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="ACHW4Z_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR8881607.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="ACHW4Z_001952"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="ACHW4Z_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR8881608.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="ACHW4Z_001953"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="ACHW4Z_001953"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8881609.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /gene="yedD"
                     /locus_tag="ACHW4Z_001954"
     CDS             complement(26368..26781)
                     /gene="yedD"
                     /locus_tag="ACHW4Z_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR8881610.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="ACHW4Z_001955"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="ACHW4Z_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR8881611.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="ACHW4Z_001956"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="ACHW4Z_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR8881612.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="ACHW4Z_001957"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="ACHW4Z_001957"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7288 a         7541 c         6963 g         7455 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtg
//
LOCUS       DAZSNG010000041        29042 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZSNG010000041 DAZSNG010000000
VERSION     DAZSNG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.042"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..90
                     /locus_tag="ACHW4Z_001958"
     CDS             <1..90
                     /locus_tag="ACHW4Z_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881613.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="ACHW4Z_001959"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="ACHW4Z_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR8881614.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="ACHW4Z_001960"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="ACHW4Z_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR8881615.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="ACHW4Z_001961"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="ACHW4Z_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR8881616.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..6990
                     /gene="fdhF"
                     /locus_tag="ACHW4Z_001962"
                     /pseudo
     CDS             4843..6990
                     /gene="fdhF"
                     /locus_tag="ACHW4Z_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            7084..7773
                     /gene="yjcO"
                     /locus_tag="ACHW4Z_001963"
     CDS             7084..7773
                     /gene="yjcO"
                     /locus_tag="ACHW4Z_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR8881617.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="ACHW4Z_001964"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="ACHW4Z_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR8881618.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="ACHW4Z_001965"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="ACHW4Z_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR8881619.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="ACHW4Z_001966"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="ACHW4Z_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR8881620.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="ACHW4Z_001967"
     CDS             complement(10475..12193)
                     /locus_tag="ACHW4Z_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR8881621.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="ACHW4Z_001968"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="ACHW4Z_001968"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR8881622.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="ACHW4Z_001969"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="ACHW4Z_001969"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR8881623.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="ACHW4Z_001970"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="ACHW4Z_001970"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR8881624.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="ACHW4Z_001971"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="ACHW4Z_001971"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR8881625.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="ACHW4Z_001972"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="ACHW4Z_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="ACHW4Z_001973"
     CDS             18432..18746
                     /locus_tag="ACHW4Z_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR8881626.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="ACHW4Z_001974"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="ACHW4Z_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR8881627.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21861
                     /locus_tag="ACHW4Z_001975"
                     /pseudo
     CDS             20570..21861
                     /locus_tag="ACHW4Z_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(22015..23664)
                     /locus_tag="ACHW4Z_001976"
     CDS             complement(22015..23664)
                     /locus_tag="ACHW4Z_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR8881628.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23815..25164)
                     /gene="ghxP"
                     /locus_tag="ACHW4Z_001977"
                     /gene_synonym="yjcD"
     CDS             complement(23815..25164)
                     /gene="ghxP"
                     /locus_tag="ACHW4Z_001977"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR8881629.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25870..26334)
                     /gene="soxR"
                     /locus_tag="ACHW4Z_001978"
     CDS             complement(25870..26334)
                     /gene="soxR"
                     /locus_tag="ACHW4Z_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR8881630.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26420..26743
                     /gene="soxS"
                     /locus_tag="ACHW4Z_001979"
     CDS             26420..26743
                     /gene="soxS"
                     /locus_tag="ACHW4Z_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR8881631.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26746..28323)
                     /gene="pdeC"
                     /locus_tag="ACHW4Z_001980"
                     /pseudo
     CDS             complement(26746..28323)
                     /gene="pdeC"
                     /locus_tag="ACHW4Z_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28752..29033
                     /locus_tag="ACHW4Z_001981"
     CDS             28752..29033
                     /locus_tag="ACHW4Z_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR8881632.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7351 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tgcgtacatg gctgaggtaa atctttataa agtaatagcg
    21361 ccatatgtta atttatttaa agccgacctt agtttctcta aactcgattt aattaacttt
    21421 gaacatgctg atctgtctcg cgtcaatctg aacaaagcaa tcctccagaa tataaactta
    21481 attgatagca aactcttttg tacgtggctg acaaatacat tcctcgaaat ggttatatgt
    21541 accggctcta atatggctaa tgttaatttt aataatgcca atttaagcaa ctgccatttc
    21601 aactgttcta ttttaacaaa agcctgtatg tttaataccc gtctctatcg ggttaatttc
    21661 gatgaggcta gcgtccaggg aatgggcatt tccattctcc gtggggagga aaatatcccc
    21721 attgatagtg ataccctggt aacactacag aaattctttg aagaagattg tacctctcat
    21781 actggcatgt cacaaactga ggataatatt aatgcagtcg ctatgaagat tactgcagat
    21841 attatgcaac acgcagattg aggttaagtc gaagaacagc caatgaattg actttacaag
    21901 agagataact tatttgtttt agaatcatga atgttcgccc gagctaacct gttaaaggtg
    21961 caaaagattg cacctttttc agattacctc gggcctggtt acgtcgcttt cggcttactg
    22021 attttcctca attagcaacg cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt
    22081 ttcgttgctg atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa
    22141 tgccgccaga cggaagcgac gctcaaggtt ctcttcctgc acggaacttt caacgtcgtt
    22201 acgtccatcg gcgcgacgac gcaggttacc aatgacgcga gaactgacct ccgtaagcaa
    22261 ctggttatcg atattctctt cggtatccgc cgccagacgc tcctccattt tctggatcgc
    22321 cacaatcgcc acttctgccg ttgccgctcg cgcaatacgt tcttctttca attgttgcga
    22381 atggtccgcg acttcaatgt gttgtagcag aataggcaac atcaccacgc cgacaaacag
    22441 cgagaagaga atgacgccag ccgccaggaa caccagctca tagcgcgccg ggaagacgtt
    22501 accatccggc aagagcagcg ggattgagag cacaccggcc agagtgatcg ccccacgcac
    22561 cccagcgaaa gacgcgatca ggatttctcg tgtggtccac gaaccaaact ccatcggctt
    22621 cttcttcagg aagcggttgc tgaacttttt catcgtccac agccagccga aacggaccag
    22681 catcagcgcc gcatatatca gaataatatc ggtaaacagc atccagattt cgacgttagg
    22741 gtcgatttct gccgccatca gcgacgtctc cagaataccc ggcagttgca gacctaacag
    22801 caggaacacc atgccgttaa agacaaattc cagcatcgcc caggtgctgt ttgcgcgcag
    22861 gcgcattgcc agtggcgcac ggcgcatcac accggagcgg gtgatggtca tcccggcggc
    22921 aaccgcagcg aggatgcccg aaacgccaat atgttcggca atcagataag aagcgaatgg
    22981 cagcaagaac agcagaacga tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa
    23041 tcgcagcgag cgaccataca gccagctcac cacaaaaccg gcaagaatac cgccaatggc
    23101 tactttcata aattcgaccg tcgcaccgcc gacagtgaag atcatcgtcc ccatcgccac
    23161 tgccacggca aacttcaacg acaccaggcc ggaagcgtca ttcatcaacg cttcgccctg
    23221 caaaatgccc atgatttttt tcgggatgcg cccttcgcca acaatcccgg agagcgccac
    23281 agcatcggtc ggagaaagta ccgccgccag cgcaaaggcg gggatcagcg gaatgcccgg
    23341 caccacccag taaataagga agccaatgcc gaccacggtg accaccacca gcgccagcgc
    23401 gaggccgaaa atctctcgac catgttcgag aaattcacgg gtcggcgttt tccagccatc
    23461 agcgaacagc aacggcggga taaacaagac taaaaagagt tcaggatcaa actccacatg
    23521 caaaccaaac gtcggccacg ccagtagcgc accgatggcg atttgcataa gcggaagcgg
    23581 gatctgaaag ggcatgacac gagtgaccac cccggacagc gagaccacga gggtcattat
    23641 cagtatggtg aagaagattt ccatgcgttc cctgtttgcg atgtttttta tgatctgaga
    23701 aagacgttat gcctgaattc tcattttttc agaatatcgc aaaagctcac acaatgtgcg
    23761 gagaaaataa aaacgggcag ccattgccac ccgttttcag aatccgatga aagattagat
    23821 agcccagcca cccgcataga aggtcaccag cgccacggcg ataacgaccg taccgatgtt
    23881 caacttgcgc cattcaccgg aaaccagacg accaatcacc agagtcgcga agccgatcat
    23941 gatgcctgtt acgatgttac aggtcagcac gatgaatacc gccgtaacca gacccgccat
    24001 cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg ctcagcatca gcaggccaac
    24061 gtacatcagc gccggagccg ttgcataccc cggaacgagg taagaaagcg gagagaggaa
    24121 cagaatcagc aggaacagca cgccaacggt gatagccgtc aacccggttt taccgcccgc
    24181 cgccgtaccc gccgcagact cgatgtatac cgctgccgga gctgcaccca ccaggccaga
    24241 gaaaacgctg ctcatggagt cagtggtcag tgctttcccg ccgtcgatga tctgcccatc
    24301 tttatccagc aggttcgcct ggccggcgac agcgcggata gttccggtgg catcaaatac
    24361 tgccgtcatc accagcgcca gaacgcttgg caggactaca ggattcagcg cgcccataat
    24421 gtccaggctg ccaatcagtg aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac
    24481 gccggagaaa tggacgttag gatcgaagat caaaccgaca attgagatac cgataatggt
    24541 cagcagaatg ccaccaggga ctttcagttt ttccaagccg atgatcaccg ccagacctgc
    24601 cagtgacata atcaccggga aggtcgcgaa atcacccagc gcaacgggca gaccatcaag
    24661 cgggttttta atcaccagac cgacaccatt agcggcaatg agcagcagga acagaccgat
    24721 accgatcccc gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc agctacggat
    24781 acccgtggca gaaattaccg taaacagcac acccatcagg aacacggcac ccagcgcgac
    24841 aggtacgcta atatgttgcc ccagcaccag gctgaatgcg gtaaacgccg tcagggagat
    24901 ggcgcaacca atcgccaacg gcagattagc ccacagaccc atcacgatag aaccgagtcc
    24961 ggcaaccaga caggttgcaa cgaaaactgc cgcaggcggg aagcctgctt tacccaacat
    25021 acctggaacg acgatgaccg agtagaccat cgccagaaac gttgttaacc cggcaaccac
    25081 ttcctgacgg acagtgcttc cacgttgtga aattttaaac caggcgtcga gtgaaccgcc
    25141 ggtacgcgct gatggcgtag acataagaaa acatcccctg agagtttaat tttcgtcaag
    25201 atgcgtggcg gacaatcccc tgccagcgaa acgttatctt cctgctccag gtttgcggca
    25261 atttggtgcg tcgcaaaaaa gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa
    25321 aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt
    25381 tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga
    25441 tgtgcaagat tgcagcgtta tcgagccgta taccgttctg gattgcgata aaaaatcgtc
    25501 tgagatgaca aaaatattgc ggctaaaaag tatactttta cactggcgaa gaaacgtcgg
    25561 tgacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg
    25621 tgttgacgtc gggggaaacc ctcctgtgtt ccagcgggat agagagaaag acaaagaccg
    25681 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt gtttgcggat aattccaaaa
    25741 gagctcgatc agtcccctcg ccccttcggg gagagggtta gggtgagggg aacaggcagc
    25801 accagtgcaa atatttaccc tcaccccggc cccatagtta acgatgagtc agcttgtcct
    25861 tgtggcgctt tagttttgtt catcttccag caagcgtgcg ccggtacctt cttctcctaa
    25921 gcggtcgccc gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca
    25981 tccgtccagt tcgtcacgca gcgccactaa ggtatgaatg cgccgatcca actcttctcg
    26041 ccattgggac gaaagctgtt tccactcttt cgcacttaac gtatgccctt ctggcaacac
    26101 gccaaacgct tcaccaatgg tcgccagcgg aatgccaata cgctgagcga ttttgataat
    26161 tgcgacatat cgcaacacat cacgtttata tcgccgctga ttgccgctgt tacggatact
    26221 agtaatcaac cctttgcttt catagaaatg cagcgccgat accgccacac cgctgcgttt
    26281 cgccacttcg ccaggggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg
    26341 ctttacctca agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac
    26401 actgaaaaga ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat
    26461 tgacgtgcat attgaccagc cgcttaacat tgatgtcgtc gcaaaaaaat caggctattc
    26521 aaagtggtac ttgcaacgga tgttccgcac ggtgacgcat cagacgcttg gcgattacat
    26581 tcgccaacgc cgcctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt
    26641 tgatatcgca atggacctgg gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg
    26701 gcagtttgat cgcactccca gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt
    26761 taactcccgc gcgggtaatt gctccatcca ttgcataaac acctgcggcg gcatcgcctt
    26821 cgcaaaaaac catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgctc
    26881 ctcagtttcg acgccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat
    26941 gtgttccgca atcaaatgac tggttttgtg ggtggtcagc gtttcaacaa acgatttatc
    27001 gattttcaaa atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc
    27061 aaaatcatca attgccactt cgtaaccagc ctggcggaaa gccagaatta atcggcgtca
    27121 ttttttcgac atcaagaaat gcatgctcag tcacttcaaa tttaatttgc tgcggacgca
    27181 ccgcgtattg ctctgttttc tgattgattc gcgctatcaa ccgtgacgta tgaaaatcgg
    27241 aggccgacag gttaatagaa acatagcgat cggcatgtgt tgccaggtac gcgcccagat
    27301 cgcggaagac attatcaata acataatcag ttatctgttc tatcatcccc tccttttctg
    27361 ccagcggaat aaactctgcc ggattcatta tttgcccctg ctcaccaggc caacgtaaca
    27421 acgcttcagc gccgatacat ttttctgttt tgatatcgat tattggctgg taataaagac
    27481 aaagttgatg tttttcgagg gcgcgttgca atttacgttt gggagataaa tagttttgtc
    27541 gaatacgtag ccagagtaat agcagaacaa gactccccaa aataccggcg ggcaacgcaa
    27601 aaataagatg attataaaaa tgggttataa gacgttgata tgaagtcgca acaatggctg
    27661 caattgggcg ttttgtcgaa taaattgtcg catataaata gccatttctt tgtacggtta
    27721 aatccttcaa atgaatcagc ggagaaaacg ttgctgccga ggcctctttg cttaacgaga
    27781 aaaaggtttt catcaccgta tcatacacac cccattgcaa tgtcgggtca tcagacatca
    27841 cttcactcca gaagagaggg ttgataaccg ccacataatt tccccgctgc atataggtca
    27901 ttttatagcc agagaaaaaa ggcgtatcgc ggtaataata gatagaaacg ttaggttcac
    27961 gcttataatc ggccggtgca atcgtatagc cgtttacagg cgctatcagc gatgagcata
    28021 aaaaatggtt atcacgaatt cattaatata aagatagcca cgaataatat tcaacattcg
    28081 cttttgatgg gctggagtgc aaacttgccc ctgatagcgt tcagctgcat cgctcactaa
    28141 atctgcctgg cgaatgacca gctcggattt atccagtgcc agttgagcaa aggttcggag
    28201 ctgattattc acttctgatt ttgcccaaag gaaggcaatc catagcgaaa gaatgatggg
    28261 aaagagaact aaaaagataa tgcccggcaa cgccagtaat tggtgtcgtg cacgatgact
    28321 cattgctatc aatttcctgc ctgtttatac ccggccattg caaggtgaaa ccctggctac
    28381 cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt gaaattgata
    28441 cctttttaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta attttctaca
    28501 ggaatacaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca cataatttgt
    28561 acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga tatagttcac
    28621 aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca
    28681 gagtgtacca cgcctcccgt gaacaacgcc gcgctgtcca gggcatcggc tcttttcaga
    28741 ggaattgttt gatggctacc ctcaccactg gcgtggttct tcttcgctgg caacttctta
    28801 gtgccgtaat gatgtttctg gccagcacac tcaacatccg ttttcgtcgg tctgattatg
    28861 tcgggcttgc ggtgatcagc agcggtctgg gcgttgtttc cgcctgttgg ttcgcaatgg
    28921 ggttgcttgg catcacaatg gcggacatca ccgctatctg gcacaacatc gagtcggtga
    28981 tgatagaaga gatgaatcag acaccgccac aatggccaat gattttgacc tgatacgtag
    29041 aa
//
LOCUS       DAZSNG010000042        28914 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZSNG010000042 DAZSNG010000000
VERSION     DAZSNG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.043"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHW4Z_001982"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHW4Z_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR8881633.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHW4Z_001983"
     CDS             complement(2138..2584)
                     /locus_tag="ACHW4Z_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR8881634.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHW4Z_001984"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHW4Z_001984"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR8881635.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHW4Z_001985"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHW4Z_001985"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR8881636.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHW4Z_001986"
     CDS             complement(5347..6009)
                     /locus_tag="ACHW4Z_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR8881637.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHW4Z_001987"
     CDS             6182..6595
                     /locus_tag="ACHW4Z_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR8881638.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHW4Z_001988"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHW4Z_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR8881639.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHW4Z_001989"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHW4Z_001989"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR8881640.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHW4Z_001990"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHW4Z_001990"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR8881641.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHW4Z_001991"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHW4Z_001991"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR8881642.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHW4Z_001992"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHW4Z_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR8881643.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHW4Z_001993"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHW4Z_001993"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHW4Z_001994"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHW4Z_001994"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR8881644.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRILVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHW4Z_001995"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHW4Z_001995"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR8881645.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHW4Z_001996"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHW4Z_001996"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR8881646.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHW4Z_001997"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHW4Z_001997"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR8881647.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHW4Z_001998"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHW4Z_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR8881648.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHW4Z_001999"
     CDS             14984..15841
                     /locus_tag="ACHW4Z_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR8881649.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHW4Z_002000"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHW4Z_002000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR8881650.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHW4Z_002001"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHW4Z_002001"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8881651.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHW4Z_002002"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHW4Z_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR8881652.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHW4Z_002003"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHW4Z_002003"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR8881653.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHW4Z_002004"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHW4Z_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHW4Z_002005"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHW4Z_002005"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR8881654.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHW4Z_002006"
     CDS             complement(22884..24023)
                     /locus_tag="ACHW4Z_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR8881655.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHW4Z_002007"
     CDS             complement(24069..26165)
                     /locus_tag="ACHW4Z_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8881656.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHW4Z_002008"
     CDS             complement(26165..26911)
                     /locus_tag="ACHW4Z_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8881657.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHW4Z_002009"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHW4Z_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR8881658.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHW4Z_002010"
     CDS             complement(27659..27964)
                     /locus_tag="ACHW4Z_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881659.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHW4Z_002011"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHW4Z_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR8881660.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7319 c         7600 g         6992 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc tggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZSNG010000043        28670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZSNG010000043 DAZSNG010000000
VERSION     DAZSNG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.044"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            164..649
                     /locus_tag="ACHW4Z_002012"
     CDS             164..649
                     /locus_tag="ACHW4Z_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881661.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACHW4Z_002013"
     CDS             646..1032
                     /locus_tag="ACHW4Z_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881662.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACHW4Z_002014"
     CDS             1057..3270
                     /locus_tag="ACHW4Z_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR8881663.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3293..3738
                     /locus_tag="ACHW4Z_002015"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACHW4Z_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACHW4Z_002016"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACHW4Z_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7538..7741
                     /locus_tag="ACHW4Z_002017"
     CDS             7538..7741
                     /locus_tag="ACHW4Z_002017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881664.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7759..8079
                     /locus_tag="ACHW4Z_002018"
     CDS             7759..8079
                     /locus_tag="ACHW4Z_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881665.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8145..8377)
                     /locus_tag="ACHW4Z_002019"
                     /pseudo
     CDS             complement(8145..8377)
                     /locus_tag="ACHW4Z_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHW4Z_002020"
     CDS             complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHW4Z_002020"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR8881666.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9438..9884
                     /gene="ivy"
                     /locus_tag="ACHW4Z_002021"
     CDS             9438..9884
                     /gene="ivy"
                     /locus_tag="ACHW4Z_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR8881667.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHW4Z_002022"
     CDS             complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHW4Z_002022"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR8881668.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHW4Z_002023"
     CDS             12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHW4Z_002023"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR8881669.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13395..14162
                     /locus_tag="ACHW4Z_002024"
     CDS             13395..14162
                     /locus_tag="ACHW4Z_002024"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR8881670.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHW4Z_002025"
     CDS             complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHW4Z_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR8881671.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15257..15778
                     /locus_tag="ACHW4Z_002026"
                     /pseudo
     CDS             15257..15778
                     /locus_tag="ACHW4Z_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15954..16451
                     /gene="rayT"
                     /locus_tag="ACHW4Z_002027"
     CDS             15954..16451
                     /gene="rayT"
                     /locus_tag="ACHW4Z_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR8881672.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16668..18380)
                     /locus_tag="ACHW4Z_002028"
                     /pseudo
     CDS             complement(16668..18380)
                     /locus_tag="ACHW4Z_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18352..19137
                     /gene="lafU"
                     /locus_tag="ACHW4Z_002029"
     CDS             18352..19137
                     /gene="lafU"
                     /locus_tag="ACHW4Z_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR8881673.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19208..20263
                     /gene="dinB"
                     /locus_tag="ACHW4Z_002030"
     CDS             19208..20263
                     /gene="dinB"
                     /locus_tag="ACHW4Z_002030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR8881674.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20315..20608
                     /gene="yafN"
                     /locus_tag="ACHW4Z_002031"
     CDS             20315..20608
                     /gene="yafN"
                     /locus_tag="ACHW4Z_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR8881675.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20611..21009
                     /gene="yafO"
                     /locus_tag="ACHW4Z_002032"
     CDS             20611..21009
                     /gene="yafO"
                     /locus_tag="ACHW4Z_002032"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR8881676.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21019..21471
                     /locus_tag="ACHW4Z_002033"
     CDS             21019..21471
                     /locus_tag="ACHW4Z_002033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8881677.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21717..22760
                     /locus_tag="ACHW4Z_002034"
     CDS             21717..22760
                     /locus_tag="ACHW4Z_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR8881678.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22822..23436
                     /gene="prfH"
                     /locus_tag="ACHW4Z_002035"
                     /pseudo
     CDS             22822..23436
                     /gene="prfH"
                     /locus_tag="ACHW4Z_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACHW4Z_002036"
     CDS             complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACHW4Z_002036"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR8881679.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25212..25670
                     /gene="gpt"
                     /locus_tag="ACHW4Z_002037"
     CDS             25212..25670
                     /gene="gpt"
                     /locus_tag="ACHW4Z_002037"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8881680.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25762..27006
                     /gene="frsA"
                     /locus_tag="ACHW4Z_002038"
     CDS             25762..27006
                     /gene="frsA"
                     /locus_tag="ACHW4Z_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8881681.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27064..27465
                     /gene="crl"
                     /locus_tag="ACHW4Z_002039"
     CDS             27064..27465
                     /gene="crl"
                     /locus_tag="ACHW4Z_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8881682.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACHW4Z_002040"
     CDS             complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACHW4Z_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8881683.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7084 a         7121 c         7606 g         6859 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccacgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg
     7501 tggtgcagat tttttctcaa aaaaacatgt cttcaaagtg gcagtttctg atctcgatga
     7561 tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg
     7621 tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag
     7681 gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc gatgtaatta
     7741 gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg
     7801 ccttgtttcc atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta
     7861 tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt
     7921 agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat
     7981 gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg
     8041 ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat atagcaacga
     8101 taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact ttcgttcagc
     8161 acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata aagttccctg
     8221 cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc gagatcttca
     8281 cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg ctgattttga
     8341 tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc tgtgttttgg
     8401 aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa tctagcccag
     8461 cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc atcccgccat
     8521 gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc atcaatacgc
     8581 gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat cacccggctg
     8641 tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac
     8701 gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct
     8761 ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacactgga
     8821 aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact
     8881 cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta
     8941 tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac
     9001 tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc
     9061 atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg
     9121 ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga
     9181 tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg caaaagcgta
     9241 atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac
     9301 cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata
     9361 tctgatttgc aaattatcgt gttatcgcta ggctttagga ggttaataac atgggcagga
     9421 taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg gtcatcgcca
     9481 ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca
     9541 aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg
     9601 gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg
     9661 cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta
     9721 atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag aaactcacct
     9781 ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga
     9841 ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa
     9901 cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc
     9961 aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc
    10021 agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat cagccccttc
    10081 gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt acccagctct
    10141 ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt
    10201 aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat acgtgaacga
    10261 gtggcgttcg gcacttgtaa aatcttcgcc attttatgat ccagcttgtc agacggtgcc
    10321 agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc
    10381 gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc atcctgtacg
    10441 ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata acgcttcaga
    10501 acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatacgctca
    10561 cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag ggcgaggttg
    10621 gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg
    10681 ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga
    10741 ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgccgcttcc
    10801 atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg
    10861 cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc
    10921 gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc catcgcatca
    10981 ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca
    11041 ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc cattacataa
    11101 gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt
    11161 ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga tttcacgccg
    11221 ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc
    11281 cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca
    11341 tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca
    11401 atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa atcttctgca
    11461 ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc
    11521 ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc
    11581 tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct
    11641 tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag
    11701 tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt
    11761 ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg cagcacacga
    11821 gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat catcgcgaag
    11881 aagcgatgct ctttaaggta cgcccacaac tccggtggca gatccgccag ctcatgagta
    11941 atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc
    12001 tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc
    12061 tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc
    12121 gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta
    12181 cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag tacccacgcc
    12241 gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata
    12301 aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc
    12361 aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg
    12421 gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca
    12481 cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg
    12541 tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa
    12601 ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct
    12661 ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt
    12721 agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg
    12781 cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta
    12841 cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt
    12901 caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg
    12961 gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa
    13021 ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg gcacggcgga
    13081 tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga ttcacattaa
    13141 agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag
    13201 gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg
    13261 acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg
    13321 ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt
    13381 tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct
    13441 gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct
    13501 ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct
    13561 ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg
    13621 ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc
    13681 gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat
    13741 cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct
    13801 ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt
    13861 ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc
    13921 tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg atcacccgcc
    13981 gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc
    14041 agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa
    14101 cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt
    14161 gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac gtaacggcca
    14221 ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc
    14281 agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc
    14341 atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag cgcaccagta
    14401 acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc gatggataca
    14461 caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc ataagcattg
    14521 gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg
    14581 acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg
    14641 ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc cattttgacg
    14701 taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac
    14761 tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca
    14821 aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt
    14881 attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa atcggaatag
    14941 tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgctg
    15001 ggtttacaaa tttagcacag gggcagtttt caatagtccg aaaagcgcat gtatcattta
    15061 tttcctaata ttatgaaatc tcaatagtaa tatgcaaata agcaattaat aatcccgttt
    15121 actcctccgg tcttttaaaa ttctcacctt cattcagccg gtttatgtct ttcatgtctt
    15181 cgttcttgct cggcagattt ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt
    15241 tactcgccag cgccaccaca accgggagca gataaaacag catccagaat ggtttcctgc
    15301 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc actttttaag
    15361 cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgcctggagc agcagcttgg
    15421 caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt
    15481 tttttatgcc tacaacaaga tccttgaggc gaaactcccg cgcacggcca atgagatgta
    15541 ccactaccgt cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctatt
    15601 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
    15661 gtttattgag tcactgcgca ccggggaaac cattcgggta agccggttag ccgaaccttt
    15721 ctggcaggac cattttttag gcgcgcgcag gattttgacg gaagagatga ttttgtaggc
    15781 cggataaggc gtttacgccg catccggcgg ttgtgcacgg atgcctgatg cgacgctgac
    15841 gcgtcttatc atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg atataacaca
    15901 gcgtcattgt cggcctttcc ttttcctctt accgttgaga aaaaggagtc gctatgtcgg
    15961 aatatcgtcg ttattacatc aaggggggaa catggttttt cacggtaaat ttacgcaatc
    16021 gtcgaagcca tcttctgacc acccagttcc agacgctccg taacgccatt attaacgtta
    16081 agcgagacag accttttgaa attaacgcct gggtcgtttt gccggagcat atgcattgca
    16141 tatggacatt acccgaaagc gatgatgatt tttcctctcg ctggcgggag attaaaaagc
    16201 agttcaccca tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca
    16261 ttcgcaatac aaaagattat cgccatcatg ttgattatat ttatataaat ccagagaaac
    16321 atggctgggt aaagcaggtg agtgattggc cattctcaac gttccatcgc gatgtcgcgc
    16381 gagggttata tcccatcgat tgggcgggag acataacgga tttaagtgcc ggggagcgta
    16441 tcattttata actgtgcgct ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt
    16501 gctttaacgg taggccggat aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc
    16561 tgatgcgacg ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga
    16621 taaggcgttt acgccgcatc cggcaattgt gcaccgatgc cggattctta catcaacgcc
    16681 cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac atgcagcccc
    16741 ggcgcaaaca accttgcata acgcggcagc aaagggcgta gctgcggcgg caccagcagc
    16801 accggatctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac
    16861 tggttgagca tattcgggtc aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc
    16921 gcctgattca ctacgttggt cagcagattt tccagctcat tattcagcgt atacaccgtc
    16981 agctcctgct tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag gcgcacatcg
    17041 gccgccagca gaatatgatc tttagtcacc gtgctactgg cgaccagcac ggtggcgatg
    17101 gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaat
    17161 tggctgtaat tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa
    17221 cggttatgca actgcgtgat gtcatcatag ttaaacaaat caggaatata gctgcgcaca
    17281 atcttgttca catgggtggc aatcacactg gcgctgtcga tcacctgata ccccatattc
    17341 agagccttcg ctttctgcgc aggctggatc caggtgaccg gtatcccgta cgccgggtcg
    17401 ttccccagca cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc catcagttta
    17461 tccgccggaa tatccgcttc atcagcctca atgccgttga tgaaaatagc gtactggctg
    17521 ggcttgaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg
    17581 gaaatcacct gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc
    17641 accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatagtt
    17701 tcccaactga cctgttgttc gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt
    17761 ttctcttccg cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc
    17821 agggtgctga acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc cagcacgaac
    17881 atgatcccgg tggcggtgta gagcaccgac gggcttgcca gcaactggtg gcgcacgtca
    17941 tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc
    18001 agcagggaag ggatctgcgc caccaggccg tcgccgatgg tcatcagcac gtattgctgg
    18061 aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc gccgctcagg
    18121 ttgatcgcca gaatcatcat cccggcgatg gcgtctccgc gtacaaactt cgatgccccg
    18181 tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc
    18241 gcctggttga tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc
    18301 gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggcctcttt ggtgacgacg
    18361 ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg
    18421 cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct
    18481 catccgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat
    18541 gtgaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagttggca
    18601 accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt
    18661 gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc
    18721 ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc gccagtgttc
    18781 gactcgctct ataacaaaat tattattacc gggcataccg atgcgatggc ctacaaaaac
    18841 aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg
    18901 gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg
    18961 ctgctggacg cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg
    19021 accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg
    19081 cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat gcgctaaatg
    19141 ctgaatcttt acgcatttct caaaacctga aatcactgta tactttacca gtgttgagag
    19201 gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat
    19261 gcgcgacaat cccgctctgc gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg
    19321 gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc
    19381 tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc gctttgacgc
    19441 ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgcattga
    19501 accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt gccacggttc
    19561 tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc aactgacggc
    19621 atctgcgggc gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc
    19681 caacggtcag tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact
    19741 ggaaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg
    19801 gacctgcggt gatgtacaaa agtgtgatct ggtgattctg cttaaacgct ttggcaaatt
    19861 tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg
    19921 gttacgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc actggtctga
    19981 atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa
    20041 acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt ttcagcagac
    20101 cacccaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa
    20161 aacctgggat gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt
    20221 gcttgacccg caaatggaaa gacaactggt gctgggatta tgatgtatac tattatgtat
    20281 attctggtgt gcattattat gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc
    20341 ggtcaatatc actgagttac gtaaaaaccc agctaaatac tttattgatc aaccagttgc
    20401 ggttctttct aataatcgcc ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt
    20461 aatggacatg cttgctgaac aagaggagaa aaagcccata aaggcgcgct ttcgtccaag
    20521 tgctgcaaga ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg atatgacgga
    20581 tgatgatttc aatgacttta aggaataagg atgcgggtat tcaaaacaaa acttattcgc
    20641 ctgcaactta cagcagagga acttgatgcg ttaacggcgg attttatttc ctataagcgt
    20701 gacggtgttt tgccagatat atttggtcgc gatgcactct acgacgactc gtttacctgg
    20761 ccattaatca aatttgagcg agttgctcat attcatctgg caaatgtgaa taatccattt
    20821 ccgccacagt tgcgccaatt cagcagaacg aatgacgaag cgcatttggt atattgtcag
    20881 ggtgcgtttg atgagcaagc atggttgctc attgccattc tgaagcctga acctcataaa
    20941 ctggctcgag ataacaacca aatgcataaa attggaaaaa tggcagaagc gtttcgcatg
    21001 cgtttttgaa tttatattat gaataacata caaataagag attatcagcc tggcgatttt
    21061 cagcaactat gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca
    21121 ctacaacaaa ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg
    21181 aaatcacaag tgcgggttgc agttattaat gcaaaactgg tcggctttat tacctgcgtt
    21241 gaacactata tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc
    21301 gctttgttaa aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc
    21361 gcaaaaccct tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc
    21421 cggggagcgt ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt
    21481 gcaatgaaaa taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga
    21541 ggtaaatcat gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct
    21601 ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa
    21661 ttcaagtcgc agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg
    21721 gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga
    21781 tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg
    21841 tagtggggat gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct
    21901 ccgcaggccg tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat
    21961 ggcaaacaga tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag
    22021 ctttggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac
    22081 agcatccgtg gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc
    22141 aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc
    22201 atctgcaact gctggttcat agcggctcga gggggctagg gcagtctatt ttacagcggc
    22261 atattgcctc gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg
    22321 cgcgttttaa tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa
    22381 gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag
    22441 gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt
    22501 cacgcggtga ttactccagg ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc
    22561 tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
    22621 aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
    22681 aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt gtggtgcaat
    22741 gtcagggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa
    22801 aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca ggggccggaa
    22861 gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa
    22921 gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac gctgcgttcg
    22981 gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact
    23041 attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg
    23101 ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg gtatgagacg
    23161 ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc
    23221 acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca gcatgctaac
    23281 aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata ggaaaatagc
    23341 gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgcga
    23401 cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc
    23461 gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt
    23521 tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag
    23581 tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg
    23641 aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt gttgaacagg
    23701 cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca
    23761 ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga atccagcatg
    23821 ctcaccacgt agtctttacc gctgtcgatt agtgaacgga tcaggcagtg aatttctacg
    23881 ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc
    23941 acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg
    24001 cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga
    24061 tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat
    24121 ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg caaaggcttc acgcgggatg
    24181 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
    24241 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
    24301 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
    24361 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
    24421 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
    24481 ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc
    24541 agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
    24601 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
    24661 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
    24721 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggttgacga
    24781 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
    24841 acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg
    24901 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
    24961 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
    25021 cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg tcagggagag
    25081 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    25141 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    25201 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    25261 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    25321 gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    25381 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    25441 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    25501 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    25561 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
    25621 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    25681 gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
    25741 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    25801 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    25861 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    25921 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
    25981 gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt
    26041 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
    26101 acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
    26161 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
    26221 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
    26281 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg
    26341 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
    26401 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg
    26461 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
    26521 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
    26581 ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt
    26641 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
    26701 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa
    26761 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
    26821 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
    26881 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
    26941 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
    27001 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
    27061 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
    27121 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
    27181 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg gtggatggag
    27241 ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt cgataaagca
    27301 ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc
    27361 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
    27421 ccggcgaatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga
    27481 tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc gacagtatca
    27541 tcattattaa tattcagctt attatcgcta tccagttggt tgattttata atcaacaaac
    27601 gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt gaccagatct
    27661 tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag cgatggacgc
    27721 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
    27781 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag ataaatatta
    27841 ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt gccacggctt
    27901 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
    27961 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
    28021 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
    28081 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
    28141 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
    28201 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
    28261 gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac
    28321 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgagtttc acctttaaaa
    28381 ccaaaacgga tataactctg gtcgccatct ttactggcgt tatcactcat ataatgcatg
    28441 gctttaactt tgccatagac atccagttta ttaccgtctt tattatatat ttctgcagcc
    28501 tgtacagatg cagatgccac aatgcccatc accactaatg ccagagtgct ctttttcatt
    28561 ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt tgaaaacagg
    28621 aaagttttta acctgagatt gttaaagata tattacagat taatgatatt
//
LOCUS       DAZSNG010000044        28420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZSNG010000044 DAZSNG010000000
VERSION     DAZSNG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.045"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            563..1333
                     /locus_tag="ACHW4Z_002041"
     CDS             563..1333
                     /locus_tag="ACHW4Z_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR8881684.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="ACHW4Z_002042"
     CDS             1324..1797
                     /locus_tag="ACHW4Z_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR8881685.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWPTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="ACHW4Z_002043"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="ACHW4Z_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR8881686.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="ACHW4Z_002044"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="ACHW4Z_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8881687.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /gene="yjjA"
                     /locus_tag="ACHW4Z_002045"
     CDS             3232..3726
                     /gene="yjjA"
                     /locus_tag="ACHW4Z_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR8881688.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="ACHW4Z_002046"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="ACHW4Z_002046"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR8881689.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACHW4Z_002047"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACHW4Z_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="ACHW4Z_002048"
     CDS             7556..8470
                     /locus_tag="ACHW4Z_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR8881690.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="ACHW4Z_002049"
     CDS             8685..10046
                     /locus_tag="ACHW4Z_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881691.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACHW4Z_002050"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACHW4Z_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR8881692.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACHW4Z_002051"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACHW4Z_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR8881693.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /gene="hpaG"
                     /locus_tag="ACHW4Z_002052"
     CDS             12596..13885
                     /gene="hpaG"
                     /locus_tag="ACHW4Z_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR8881694.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="ACHW4Z_002053"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="ACHW4Z_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR8881695.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="ACHW4Z_002054"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="ACHW4Z_002054"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR8881696.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="ACHW4Z_002055"
     CDS             16211..16591
                     /locus_tag="ACHW4Z_002055"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR8881697.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="ACHW4Z_002056"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="ACHW4Z_002056"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR8881698.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="ACHW4Z_002057"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="ACHW4Z_002057"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR8881699.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="ACHW4Z_002058"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="ACHW4Z_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR8881700.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="ACHW4Z_002059"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="ACHW4Z_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR8881701.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="ACHW4Z_002060"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="ACHW4Z_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="ACHW4Z_002061"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="ACHW4Z_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="ACHW4Z_002062"
     CDS             23320..23832
                     /locus_tag="ACHW4Z_002062"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR8881702.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="ACHW4Z_002063"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="ACHW4Z_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR8881703.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="ACHW4Z_002064"
     CDS             26425..26628
                     /locus_tag="ACHW4Z_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8881704.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="ACHW4Z_002065"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="ACHW4Z_002065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR8881705.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28420)
                     /locus_tag="ACHW4Z_002066"
     CDS             complement(27664..>28420)
                     /locus_tag="ACHW4Z_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR8881706.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6957 a         7426 c         7126 g         6911 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggccaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac acagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gatatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtataa
//
LOCUS       DAZSNG010000045        28374 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZSNG010000045 DAZSNG010000000
VERSION     DAZSNG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.046"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHW4Z_002067"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHW4Z_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHW4Z_002068"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHW4Z_002068"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR8881707.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHW4Z_002069"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHW4Z_002069"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR8881708.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHW4Z_002070"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHW4Z_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHW4Z_002071"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHW4Z_002071"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR8881709.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHW4Z_002072"
     CDS             complement(5877..6218)
                     /locus_tag="ACHW4Z_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR8881710.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHW4Z_002073"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHW4Z_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR8881711.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHW4Z_002074"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHW4Z_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR8881712.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12583
                     /gene="msrA"
                     /locus_tag="ACHW4Z_002075"
     CDS             11936..12583
                     /gene="msrA"
                     /locus_tag="ACHW4Z_002075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR8881713.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGGIGVCLPPEA"
     gene            12906..14249
                     /locus_tag="ACHW4Z_002076"
     CDS             12906..14249
                     /locus_tag="ACHW4Z_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR8881714.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14311..14517)
                     /locus_tag="ACHW4Z_002077"
     CDS             complement(14311..14517)
                     /locus_tag="ACHW4Z_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR8881715.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14842..15396
                     /locus_tag="ACHW4Z_002078"
     CDS             14842..15396
                     /locus_tag="ACHW4Z_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8881716.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15459..16397)
                     /locus_tag="ACHW4Z_002079"
     CDS             complement(15459..16397)
                     /locus_tag="ACHW4Z_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881717.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16609..17349)
                     /gene="cysQ"
                     /locus_tag="ACHW4Z_002080"
     CDS             complement(16609..17349)
                     /gene="cysQ"
                     /locus_tag="ACHW4Z_002080"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR8881718.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17539..19482
                     /gene="cpdB"
                     /locus_tag="ACHW4Z_002081"
     CDS             17539..19482
                     /gene="cpdB"
                     /locus_tag="ACHW4Z_002081"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR8881719.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19608..19988)
                     /locus_tag="ACHW4Z_002082"
     CDS             complement(19608..19988)
                     /locus_tag="ACHW4Z_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR8881720.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20077..20937
                     /gene="qorB"
                     /locus_tag="ACHW4Z_002083"
     CDS             20077..20937
                     /gene="qorB"
                     /locus_tag="ACHW4Z_002083"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8881721.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21046..22011
                     /locus_tag="ACHW4Z_002084"
     CDS             21046..22011
                     /locus_tag="ACHW4Z_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8881722.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22119..22781
                     /gene="ytfE"
                     /locus_tag="ACHW4Z_002085"
     CDS             22119..22781
                     /gene="ytfE"
                     /locus_tag="ACHW4Z_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR8881723.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22826..24238)
                     /gene="cycA"
                     /locus_tag="ACHW4Z_002086"
                     /gene_synonym="dagA"
     CDS             complement(22826..24238)
                     /gene="cycA"
                     /locus_tag="ACHW4Z_002086"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR8881724.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24546..25166)
                     /gene="fklB"
                     /locus_tag="ACHW4Z_002087"
     CDS             complement(24546..25166)
                     /gene="fklB"
                     /locus_tag="ACHW4Z_002087"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8881725.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25385..25930
                     /locus_tag="ACHW4Z_002088"
     CDS             25385..25930
                     /locus_tag="ACHW4Z_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR8881726.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25914..26561)
                     /locus_tag="ACHW4Z_002089"
     CDS             complement(25914..26561)
                     /locus_tag="ACHW4Z_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8881727.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26773..27564
                     /locus_tag="ACHW4Z_002090"
     CDS             26773..27564
                     /locus_tag="ACHW4Z_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR8881728.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27574..>28374
                     /locus_tag="ACHW4Z_002091"
     CDS             27574..>28374
                     /locus_tag="ACHW4Z_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR8881729.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         6962 a         7553 c         6996 g         6863 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc ggaattggcg tctgtctgcc gccggaagca tagcgttacg ggtacaaatg
    12601 tagtttgttg ataaagtgct ctttgtctat gcctgatgcg gcttaaacga cttaccaggc
    12661 ctacaaaatc gcccaaattc aatatattgc aaggactgcg taggcctgat cggcatagcg
    12721 catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg actttacagt
    12781 accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat
    12841 gtgtatttca cacaaattaa tcaactttcc cttccgaggg tctggcctga aaggtcggat
    12901 aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt gcgttcttct
    12961 cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg ctggctgatg
    13021 aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc atgttcttta
    13081 ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg
    13141 cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagag ctctctgagc
    13201 aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg tttgcggatt
    13261 taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt atcatcaacc
    13321 cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac ggcctggcga
    13381 acatgatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc acttctgatg
    13441 acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg
    13501 agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca atgacaccgc
    13561 gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag aataaggtgg
    13621 cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac atcatcggtt
    13681 atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca
    13741 gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt tcagaggcgt
    13801 tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag tacgcgctgg
    13861 tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat ctggtcggtc
    13921 aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt gacggcggca
    13981 ccccaattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag tcgggcaact
    14041 acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt
    14101 cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac cgcatcgacc
    14161 agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg
    14221 ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct cctgaatcag
    14281 gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca gacgcataac
    14341 ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt ttgggataag
    14401 aatcttaccc ttatcaaatt caaaagcgcc aatgtccttg atgtataacc gtccacggaa
    14461 caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa ttttcattgt
    14521 tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg
    14581 caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg cgtataactt
    14641 tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc ggtcttcagt
    14701 ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat cacacttgcg
    14761 ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg gatcacctgc
    14821 atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac ctgcctgctg
    14881 ttgccgatga tggcttccgc acatcagttc gaaatcggtc agcgagtgcc gccgattggc
    14941 attaccgatc ggggcgagtt ggtgcttgat aaagatcagt ttagctacaa aacctggaac
    15001 agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg cacctctgca
    15061 aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc gcacgatcgt
    15121 taccagacca ccaccattgt taacaccgac gacgcgattc cgggttccgg catgtttgtg
    15181 cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat tgtcgatagc
    15241 aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt agtggtgctg
    15301 gataaagacg gtcgcgtgca atgggccaaa gacggggcgc tcacaccgga agaggtgcag
    15361 caagtgatgg acctgctgca aaagttgctc aaataatggc aataacataa attctggaaa
    15421 aataattttc ccctatcgtt atctatggga taggggaatt aagtctctga gttcttacac
    15481 cgaactaata ctggtaacgc tagttgctct tttttaaacg cctcaatggt cgatttatat
    15541 ttctcgcaaa tcttttcttt ggtaatatta ctatctgcgg ccagattcgc acttactatc
    15601 gcatccactt gttcggctaa ttcgacttgt tggcaacctg cattaaccag aaaattataa
    15661 ataagagagt aatcttcatc ctccaaaaca acagaggaac cattacgaaa acgttcatcc
    15721 cctccataga ttttaagcgc aaagcccaaa gattctatga ctatggcaaa attatcatca
    15781 attatttttt cgtaaatata ccatccatcg cattctttaa ttccaccgcc tggtatttgg
    15841 agttcaaatt taccccgttt taatttgctt actacatcct ccaataaggc ataaccgaaa
    15901 gatacgtcac caaacgcctg gcgaatataa cctttaatcc tttttggtat gtcagactgt
    15961 tttttctgca aaatatcaca ggcatacaat cttctgtttt gttctaacgg aaactcgcgg
    16021 tttttgaaat ccatgaatga gttatactca tttattgtta tgggaatttc ttcgtattca
    16081 aacccactgc ctcgcaatgt tattgtatat tcagcaccgt tatctttaag gctgaaactg
    16141 ataagaaaat tatccatggc attaacagtt tcattttttg atgaaagcac ttgataacta
    16201 tttttatcaa tctgtgttgc gcaacgaaag gactcccgca aagttaacag actatcataa
    16261 ttcgaaaccc aatctttttt cttaaatgca tgctgaatat tattaaaaaa atcgtttgaa
    16321 tgtgtttttg tatgcgatga ctttacaaat aactccaccg caccaataaa attattgtgt
    16381 ctgatcctgg gtaacatatg gctaccttat tttatttggt tattttatgt cgccattaaa
    16441 tatctcatta tagaagtaaa ctatcaaaaa acgctcatta ttaaaatgta acttactagc
    16501 aattgaattt ttgcacttta aatacaatct ctttttattt ataacttttt aggttgggat
    16561 aaatcaggat ggcagaatca ggaaatacac tgtttctgcc atctgaattt agtaaatgga
    16621 cactctgaac cccggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg
    16681 ccagtcgtga acgtgcgctc cggcagctgc agccacagca tgtccggcgg cggtgtccca
    16741 aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca ccaggcagaa
    16801 tttcagcgaa gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt
    16861 cagctccgca tctgcatggg aacggctgat aaccaccagc ggcgggcgcg catcgcggac
    16921 ctgaatcagc ttgcgcacac cgcactcttc tttccaggcc ttgccttctg ccgcgctgta
    16981 cattacgttc attaccggcg catacaccac acctaaaatc ggtttgccat ggtcaatgag
    17041 cgcaatgtta acggtgaatt cgccattacg tttaataaac tctttagtac catccagcgg
    17101 gtctaccagc cagtaacgct gccagtgctg acggacttcc caaccgggag gatcttcttc
    17161 agaaaggacc ggaatatccg gtgccagcgt acgtaaaccg tccatgataa cggtgtgagc
    17221 ggcaatatcc gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt
    17281 cgtcccgtcg tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg
    17341 atctaacatt tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc
    17401 ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg
    17461 gcattcttca ctgttctata agtaaggcgt ttattctccc ttttctttcg tattcccgat
    17521 gataaaagga tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc
    17581 gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg aaaccactga tctgcatagc
    17641 aacatgatgg atttcgatta ttacaaagac accgccacgg aaaaattcgg actggtacgt
    17701 acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa acagcgtact ggtcgataac
    17761 ggcgatttga ttcaggggag tccgctggcc gattacatgt cggcgaaagg attaaaagca
    17821 ggtgatgttc atccggttta taaggcgctg aatacgctgg attatacggt cggtacactc
    17881 ggcaatcatg aatttaacta cggtctggat tacctgaaaa atgcgctggc gggagcgaaa
    17941 ttcccttatg taaatgccaa cgtcattgac gccagaacca aacagccaat gtttacaccg
    18001 tatttaatta aagataccga agtggtcgat aaagacggaa aaaaacagac gctgaagatt
    18061 ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg ataaagctaa tttatccgga
    18121 aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga aatgcgcgag
    18181 aaaggtgccg atgtcattgt cgttctggcg cattccgggc tgtctgccga tccgtataaa
    18241 gtaatggcgg aaaactcagt ttattacctc agtgaaattc cgggcgttaa cgccattatg
    18301 tttggccatg ctcacgccgt tttcccgggt aaagattttg ctgatatcga aggggctgat
    18361 atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg gggcgatcat
    18421 cttggggtgg tcgacttaca actcagtaat gacagcggta aatggcaggt gacgcaggcg
    18481 aaagcggaag cacggccgat ttacgacatc gccaataaaa aatccctcgc ggcggaagac
    18541 agcaagctgg tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag
    18601 ccaatcggta aatctgccga caatatgtat agctatctgg cgctggtgca ggacgatccg
    18661 accgtgcaag tcgtgaacaa cgcgcaaaaa gcgtatgtcg aacattacat tcagggcgat
    18721 ccggatctgg caaaactgcc ggtgctttca gctgccgcac cgtttaaagt tggtggtcgc
    18781 aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc
    18841 gccgatcttt atctctaccc caatacgctg attgtagtga aagccagcgg taaagaggtg
    18901 aaagagtggc tggagtgctc cgccggacag tttaaccaga ttgctcctaa cagcacgaaa
    18961 ccacagtcac tcatcaactg ggatggtttc cgcacttata actttgatgt tattgatggt
    19021 gtgaattatc agattgatgt tacccaaccc gcccgctatg acggcgagtg ccagatgatt
    19081 aatgccaatg cagagcggat taagaacctg acctttaatg gcaagccgat tgatccgaac
    19141 gctatgttcc tggttgccac caataactat cgcgcttacg gcggcaaatt tgccggtacg
    19201 ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc gctcggtgct ggcagcgtgg
    19261 attgctgatg agtcgaaacg tgcgggggaa attcatccgg cggcagataa caactggcgt
    19321 ttagcaccga tagctgccga taagaaactg gatatccgtt tcgaaacttc cccgtcagat
    19381 aaagccgcag cgtttattaa agagaaaggg cagtatccga tgaataaagt cgcgaccgat
    19441 gatatcgggt ttgcgattta tcaggtggat ttgagtaagt aaaacacttc tttttcggcc
    19501 tataaatcaa ccgcatccgg catttattgg cggatgcgat gctgccgtat cttatccacc
    19561 ctacaagcca tgaaccgtag accggataag cgcagcgcat ccggcagtta tgccgcacgt
    19621 tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc cagtgcagca
    19681 accttttcgc tcacctgctc gcccagcggc gtgaggctat attccacatg cggcggcacc
    19741 accggatacg cgatacggtt aagaaaacca tcctgttcta acgcctgtaa cgactgcgca
    19801 agcatctttt cactcacccc accgattttg cgccgcaggt cgctaaagcg atgagtacct
    19861 tcgcgtagcg ccaccagaat caacaccccc caacggctgg tgacgtgttt caacacctcg
    19921 cgcgacgggc actgttccgc aaagagatta ccctctttca gttgttgtga caggctaacc
    19981 tgactcattt catacttacc tttttgcacg tacttactaa aagtaagttt aggtgttagc
    20041 gtatttaaac acaagacaaa acgatggaga cttcccatga tcgctattac tggtgccact
    20101 ggccaacttg gtcactatat tattgaatcc ttgatgaaaa cggttcctgc cagccaaata
    20161 gtggctatcg ttcgtaatcc ggcaaaagcc caggccctgg cagcacaagg cattaccgtg
    20221 cgtcaggctg actacagcga tgaagccgca ctgacatctg cacttcaggg agtggaaaaa
    20281 ctactgctga tctcttccag cgaagtgggt caacgtgccc cgcagcatcg taatgttatt
    20341 aatgccgcaa aggcggctgg cgtgaaattt atcgcttata ccagcctgct acatgcagat
    20401 acctccccgc tcggcctcgc cgatgagcac atcgagacgg agaaaatgct ggctgattct
    20461 ggcatcgttt acaccctgct gcgcaacggc tggtacagcg aaaactacct cgccagcgcc
    20521 ccggcagcac tggaacacgg cgtatttatc ggtgcggctg gcgatggcaa aatcgcctca
    20581 gcaacgcggg cagattatgc gacagctgcg gcacgcgtga ttagcgaagc cggtcacgaa
    20641 ggcaaggttt acgaactggc gggcgatagt gcctggacgt tgacacagtt agcggcagag
    20701 ctgaccaaac agagcggcaa acaggttacc tatcaaaatc tgagcgaagc cgatttcgcc
    20761 gcggcactga aaagcgtcgg actgcccgac ggactggcgg atatgctggc ggattctgac
    20821 gttggcgcat cgaaaggtgg tctgtttgat aacagcaaaa cgcttagcaa attgattggc
    20881 tgcccaacga caacgttagc cgaaagcgta agccatcttt ttaatgttaa taactagtta
    20941 attaaagtgg catcctcccg catcctctct gataatgacg ggatgccggg agcaatcatg
    21001 tctgcttcct gaacgtttct tctgacagac caatggatgc cagtaatgat tagcggcgtg
    21061 ctgtacgccc tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgtg
    21121 ccggaatacc cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg
    21181 ctacccattg cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg ggactggctt
    21241 accgccttga tgctcactat gatgggcaac ctcatttatt acttctgcct tgccagtgcc
    21301 attcaacgta ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt
    21361 cctgtctttg ccaatctgct ttatagccag cgcgacggca aactcgcgtg gggaaaactc
    21421 gccccggcac tgatttgtat tggcatcggc ctggcgagtg tgaatattgc tgagttaaac
    21481 cacggactcc ccgattttga ctgggcacgt tatacctctg gcatcgtgct agcgttagtt
    21541 tccgtggtct gctgggcatg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc
    21601 gacaaacatc cgatgatgtg ggcgacggcg caggcgctgg tcacgctgcc ggtttctctc
    21661 atcggctatc tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct
    21721 tttggccccc gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca
    21781 tgggttggcg cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg
    21841 ccgctgattg ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa
    21901 atgccgccgc taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcatcgcg
    21961 gtcagagcaa aaccagaaaa acctttaact gaatctgtct cagaaagttg acatgctggc
    22021 agtgagttaa ataagcctct gctacgtaag ggttatagct tttgccttaa aggtgtattt
    22081 aaaatacatc ttatcttatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt
    22141 taggtgaact ggcgctctct atccctcgcg cttcggctct gtttcgtaaa tatgatatgg
    22201 attactgctg tggcggtaag cagacgctgg cgcgcgcggc ggcacgtaaa gaactggatg
    22261 ttgaggtcat tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc
    22321 gtagcgcccc gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc
    22381 gcgagcagct gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca
    22441 aaccgagcgt gccaaaaggg ctgacaaaat acctgaccat gctgcatgaa gagctttcca
    22501 gccacatgat gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc
    22561 aggcaatggg gccaatcagc gtaatggaaa gcgagcacga tgaagcgggc gaactgctgg
    22621 aagtgatcaa acacactacc aataacgtca caccgccgcc agaagcctgc accacctgga
    22681 aagcgatgta taacggcatt aatgaactga ttgatgacct gatggatcac atcagtctgg
    22741 aaaacaatgt actgttccca cgcgccctag cgggtgagtg atctaaaagc tggatggcat
    22801 tgcgccatcc agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa
    22861 taaacagcca gcccaacccc agcgcgataa accacagcgg ggtaaccagc agcgcctggc
    22921 gagtgtcatc ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc
    22981 agcacatcag cttgccgagc ggcatcttgt agatcgactt ttcatgcaga tgaggacgct
    23041 gtttgcggta caccaggtaa gagcaaagga taatcgtcca gacgaacata aacagaatcg
    23101 cggaaacggt tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca
    23161 ccacgccgcc gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct
    23221 tagaaagttt agcgaacgct ttcggcgcaa caccttcctg cgccagacca aacagcatac
    23281 ggctggtaga gaagacaccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt
    23341 tgatcacgct ggcagccgca ggcaaaccta ccaacacaaa cagctcaacg aacgggcttt
    23401 tctccgggac taccgaactc cacggtgtca cggacataat cacaatcagc gcgaagacgt
    23461 agaacataat gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat
    23521 ctttggtttc cgcagctgtt gtacctacca gctcaatccc cacgaaagcg aaaaccgcta
    23581 tctggaatcc ggcaaagaag ccacttaaac ctttcgggaa ccagccgccg tcattccaca
    23641 aatgcgcgaa tgacgcttcc acaccggtcg gtgactgaaa gtgcatcgcc accatgacca
    23701 ggccgacgac aatcagcgac acgatggcga cgattttgat catcgcaaac cagaactcca
    23761 tctcaccgaa cattttcacg gtggcaaggt tgagcgtcag cagcagcact atcaccgcca
    23821 gcgaggcgac ccagtcggag agatcgggga accagaactg agcataagcc gtgatcgcca
    23881 ccacgtctgc cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc
    23941 ccgcccacgg ccccagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg
    24001 aaagcagcaa ttcccccatt gcccgcatca cgaaaaagag cataaaacca atgatcatat
    24061 aaacgaaaat gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc
    24121 cagtaccaat ggcacctcca atggcaataa gctgaacatg tcggtttgtg agattgcgcc
    24181 gtagcgactg ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt
    24241 tttttcttcc tgtacctgtc tgtgttgttc aggctctacg gcctttagtc agtctatgat
    24301 acgacgaatc tgtatcggga ctcatcgata ttaggtaaca atacgcggga tgaatactaa
    24361 gatttagatc taatgttaat tttatgttta aagtgagtgt catatcacgg tgataacgcg
    24421 aaacagctca caaaaataca cgcttacggg gtctttgcaa cacaaaatag cgccgtttgg
    24481 caaactgaag ggtttattgc tgaatgcctg ctcccctctg gttcgagggg aacagaatgc
    24541 gctgcttaga ggatttccag cagttcgact tcaaacacca gggtgctgaa cggagggatg
    24601 gatgcacctg cgccgcgctc gccatatgcc agttcctgcg ggatagtcag ttcccattta
    24661 gaacctaccg gcatcagagt cagtgcttca atccagccag ggatcacgcc attaaccggg
    24721 aattcagcgg gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc gatcagttta
    24781 ccggtgtaat gaacacgaac gcggtcggta cgtgccggaa ttgcgccttc accctggttg
    24841 atcacgcgga attgcaggcc agattcggtg ctattcacac cttctttttt ggcgttttct
    24901 tccaggtatt tcacgccttc agcagccatc gcctggaaac gctgacgacg aacggcatcg
    24961 gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat caaccggcac agccggatgt
    25021 ttgccttcca gcgcatcggc aatacctgca accagtgctt ctggcagcag cccttccagg
    25081 ccagattcac tcagttgttg ccctacctgc aaaccaatgc cgtagcttgc ttgcgcttcg
    25141 atggtgtcaa aagttggggt ggtcatcgta tttcctttct tagggtataa aagtagcggg
    25201 cagcatatca gccacacttc tttgggtaaa actttgtcgc aggaatgatg acaaatcccg
    25261 gggaaaaaga aacaatagca atatcccgta tgaatataat aaaggcgggt ttaccacgga
    25321 catcatagcg ttagcttatt cataggctat gattgaggaa caagacgtgg agcaggagga
    25381 aaccatgccc gggcgctttg aactaaaacc aaccctggag aaagtctggc acgcgccgga
    25441 caattttcgc ttcatggacc cgctgccgcc gatgcatcgt cgggggatca ttattgccgc
    25501 cattgtattg gtggtcggat ttctgcttcc atctgatgat acgcccaacg cgtcggtggt
    25561 gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa ccgccgacag aagaacagcc
    25621 gcaaactacc caaacgcaac cattccagcc agatagcgga attgataatc aatggcgctc
    25681 ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cgcgaccacg gactgcccgc
    25741 taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga gtaatttgca
    25801 aaatgggcag atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg gcttaaccat
    25861 tgatacgggg aataatcaac aggtcctgtt tacccgccag ccggatggca gttttattcg
    25921 tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt gatgacctta
    25981 cgtctggctt ccagtgcctg gggggaaacc atactgatat gataaactag gccaagcgta
    26041 tgctggttga tcaaataata tcccccaagc gcagcaatat taatattcac ctgcaatggg
    26101 tcgatacctg gcttaaaaat attgtatttc ttcccttcat ccaataagga ttccattaac
    26161 tgcaagtgag catggttaat ttctaagaga cgctgtgatt ttgcataatg cacaccttta
    26221 ctttgatttt cactatgcac aatttttaaa aaccaaggat tagcaatata ataatcccat
    26281 gtaaaatcaa taattttctc aatggctttt tgtggcggaa gatctgagaa atcaagggcc
    26341 ttttcttttt gtcgaatatc gttccaggta tattccagca cttcgataaa taaattttct
    26401 ttattaccaa agtggtgata aaccgtctgt ttactacacc ctgctgcctt tacaatatta
    26461 tctactctgg ccccttcata accatattcg gcaaattcat taaccgcaca tagcagcaat
    26521 ttttccttca ggctctgtac gttgctggta tcaatataca taacgcttcc ccacgacctc
    26581 ttcgtggtga ctatcataac ctgatttgat ttcagcttca gaaaaaaggg caaaaacttg
    26641 tactttcatc aggttatcaa atgtcctgtg atcgccatcg catatcaacc aactagatgg
    26701 tttatctttt tatcctccag tcgcaagcta atcatcacca aggaaacaaa attcaaaaaa
    26761 gagagctatt gtatgaaatc cactcgtcct gttgccgtga ttaccggagc cactcgcggt
    26821 attggaaagg gctgtgcgca ggagcttgct cgtggcgggt ttaacctgct gattaacgat
    26881 cgtcccgatg ccgacagcgt agaaaaactg catatcacac aacaagaatg tttggcggaa
    26941 ggcgtggaag tgatttgctt ccctgctgat gtcggtgatc tatctctgca tgaggagatg
    27001 ctcgacgcgg cgcaaaacca gtgggggcgg ctggactgtc tgctcaataa cgcggggatt
    27061 tcagtgaaaa aacgcggtga cctcctcgac ctcgaaccag acagcttcga tcaaaacatt
    27121 gccattaaca cccgcgcgcc gttcttcctt gctcaggcat tcagtaagcg cttactggca
    27181 cagccgaaac cggaagcgga gctgccgcac cgctcgatta ttttcgtcag ttccatcaac
    27241 gccatcatgc tggcgatgaa tcgcggcgaa tacaccattg ccaaaaccgc cgtttctgcc
    27301 gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa ttggcgttta tgaagtgcgc
    27361 cctggtctca tcaaaactga tatgactatt ccggcaacgg cttattacga cgaactgatt
    27421 gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg ctgatattgc ttccaccgtc
    27481 cgcgcgatgg cggaaggtaa actgatttac acctgtggtc aggcggtcgc aatcgacggc
    27541 ggcttaagta tgccgcgctt ctgaggctga atcatgagag atctgttaca acgcccggat
    27601 ttattcagta tcaataccgc gacactgggc tataaaacgc cgctacccgc cattattgat
    27661 gcctgcgccg cacgcggtat tggcgcaatt gccccctggc gcagggagtt gcagggtgaa
    27721 gatctccagc aaatcacccg ccagcttgcc gccagtaaca tgagcgtttc cggtttgtgc
    27781 cgtagcactt actacaccgc gccgacactg gcggaacgca aactggcgat tgatgataac
    27841 cgccgggcgc tggacgacgc agcggtgctt aacgccgcct gttatatgca ggtggttggc
    27901 ggtctgccaa cgggcactaa agatctatat gaagcgcgag aacaagtgaa acaagggatt
    27961 cgccagttgc tgccacattc gaaagatgtc ggcgtgccaa ttgcgctgga gccactgcac
    28021 ccgatgacag ctgccgatcg ctcttgcctg tgcacgttac gtcaggcgct ggactggtgt
    28081 gatgaactcg atcccgatgg tgagtttggc ctcggcgtgg cggtggatgt ttatcacgtc
    28141 tggtgggatc cggatctcgc cagtcagatc ctgcgtgccg gaaaacgcat cctcgcgttt
    28201 catgtttccg actggttaat gccgaccact gatctggtca atgaccgtgg tatgccggga
    28261 gatggcgtca tcaatattcc gtcaattcgt cggctggttg aaaacgccgg atttaatggt
    28321 gctattgaac tggaaatatt ctcgccttac tggtggcaga aagatattaa cagc
//
LOCUS       DAZSNG010000046        28352 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZSNG010000046 DAZSNG010000000
VERSION     DAZSNG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28352
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.047"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..246
                     /locus_tag="ACHW4Z_002092"
     CDS             <1..246
                     /locus_tag="ACHW4Z_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8881730.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHW4Z_002093"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHW4Z_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR8881731.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHW4Z_002094"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHW4Z_002094"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8881732.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHW4Z_002095"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHW4Z_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR8881733.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHW4Z_002096"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHW4Z_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR8881734.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHW4Z_002097"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHW4Z_002097"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR8881735.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHW4Z_002098"
     CDS             complement(4442..5650)
                     /locus_tag="ACHW4Z_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881736.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHW4Z_002099"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHW4Z_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR8881737.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHW4Z_002100"
     CDS             complement(6445..8130)
                     /locus_tag="ACHW4Z_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR8881738.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHW4Z_002101"
     CDS             8400..8777
                     /locus_tag="ACHW4Z_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR8881739.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHW4Z_002102"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHW4Z_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR8881740.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHW4Z_002103"
     CDS             9224..9511
                     /locus_tag="ACHW4Z_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR8881741.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHW4Z_002104"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHW4Z_002104"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR8881742.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHW4Z_002105"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHW4Z_002105"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR8881743.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHW4Z_002106"
     CDS             11268..11744
                     /locus_tag="ACHW4Z_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR8881744.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHW4Z_002107"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHW4Z_002107"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR8881745.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="ACHW4Z_002108"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="ACHW4Z_002108"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR8881746.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="ACHW4Z_002109"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="ACHW4Z_002109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR8881747.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="ACHW4Z_002110"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="ACHW4Z_002110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR8881748.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="ACHW4Z_002111"
     CDS             16301..16789
                     /locus_tag="ACHW4Z_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR8881749.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="ACHW4Z_002112"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="ACHW4Z_002112"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR8881750.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACHW4Z_002113"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACHW4Z_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR8881751.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACHW4Z_002114"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACHW4Z_002114"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR8881752.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACHW4Z_002115"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACHW4Z_002115"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR8881753.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACHW4Z_002116"
     CDS             complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACHW4Z_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR8881754.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACHW4Z_002117"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACHW4Z_002117"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR8881755.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="ACHW4Z_002118"
     CDS             complement(22241..22756)
                     /locus_tag="ACHW4Z_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8881756.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="ACHW4Z_002119"
     CDS             23405..25174
                     /locus_tag="ACHW4Z_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8881757.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="ACHW4Z_002120"
     CDS             25385..25708
                     /locus_tag="ACHW4Z_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR8881758.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /gene="amiD"
                     /locus_tag="ACHW4Z_002121"
     CDS             25705..26535
                     /gene="amiD"
                     /locus_tag="ACHW4Z_002121"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR8881759.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="ACHW4Z_002122"
     CDS             complement(26532..27545)
                     /locus_tag="ACHW4Z_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8881760.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..>28352)
                     /locus_tag="ACHW4Z_002123"
     CDS             complement(27644..>28352)
                     /locus_tag="ACHW4Z_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR8881761.1"
                     /translation="ISVWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLI
                     AFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWL
                     VVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVDSWKVIVVEP
                     KKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFI
                     FRGMAKQIARLAEQSTD"
BASE COUNT         7130 a         6831 c         7230 g         7161 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccatgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaaa taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tc
//
LOCUS       DAZSNG010000047        27601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZSNG010000047 DAZSNG010000000
VERSION     DAZSNG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.048"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHW4Z_002124"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHW4Z_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR8881762.1"
                     /translation="MAESTVTADSKLTSSDTRHRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHW4Z_002125"
     CDS             complement(1614..1937)
                     /locus_tag="ACHW4Z_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR8881763.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHW4Z_002126"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHW4Z_002126"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR8881764.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHW4Z_002127"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHW4Z_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR8881765.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHW4Z_002128"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHW4Z_002128"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR8881766.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHW4Z_002129"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHW4Z_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR8881767.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHW4Z_002130"
     CDS             7537..8574
                     /locus_tag="ACHW4Z_002130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8881768.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHW4Z_002131"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHW4Z_002131"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR8881769.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHW4Z_002132"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHW4Z_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR8881770.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHW4Z_002133"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHW4Z_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR8881771.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHW4Z_002134"
     CDS             10745..11626
                     /locus_tag="ACHW4Z_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8881772.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHW4Z_002135"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHW4Z_002135"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR8881773.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHW4Z_002136"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHW4Z_002136"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR8881774.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHW4Z_002137"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHW4Z_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHW4Z_002138"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHW4Z_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR8881775.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHW4Z_002139"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHW4Z_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR8881776.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHW4Z_002140"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHW4Z_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR8881777.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHW4Z_002141"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHW4Z_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR8881778.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHW4Z_002142"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHW4Z_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR8881779.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHW4Z_002143"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHW4Z_002143"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR8881780.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHW4Z_002144"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHW4Z_002144"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR8881781.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHW4Z_002145"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHW4Z_002145"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR8881782.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHW4Z_002146"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHW4Z_002146"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR8881783.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHW4Z_002147"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHW4Z_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR8881784.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHW4Z_002148"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHW4Z_002148"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR8881785.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHW4Z_002149"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHW4Z_002149"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR8881786.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHW4Z_002150"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHW4Z_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR8881787.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHW4Z_002151"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHW4Z_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR8881788.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHW4Z_002152"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHW4Z_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6865 c         7236 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcaccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZSNG010000048        27399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZSNG010000048 DAZSNG010000000
VERSION     DAZSNG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.049"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..574
                     /locus_tag="ACHW4Z_002153"
     CDS             <1..574
                     /locus_tag="ACHW4Z_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8881789.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="ACHW4Z_002154"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="ACHW4Z_002154"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR8881790.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACHW4Z_002155"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACHW4Z_002155"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR8881791.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACHW4Z_002156"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACHW4Z_002156"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR8881792.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACHW4Z_002157"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACHW4Z_002157"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8881793.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACHW4Z_002158"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACHW4Z_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR8881794.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="ACHW4Z_002159"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="ACHW4Z_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR8881795.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="ACHW4Z_002160"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="ACHW4Z_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR8881796.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="ACHW4Z_002161"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="ACHW4Z_002161"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR8881797.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="ACHW4Z_002162"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="ACHW4Z_002162"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR8881798.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="ACHW4Z_002163"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="ACHW4Z_002163"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR8881799.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="ACHW4Z_002164"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="ACHW4Z_002164"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR8881800.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="ACHW4Z_002165"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="ACHW4Z_002165"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR8881801.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACHW4Z_002166"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACHW4Z_002166"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR8881802.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="ACHW4Z_002167"
     CDS             13934..14314
                     /locus_tag="ACHW4Z_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR8881803.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="ACHW4Z_002168"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="ACHW4Z_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR8881804.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACHW4Z_002169"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACHW4Z_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR8881805.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACHW4Z_002170"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACHW4Z_002170"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR8881806.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="ACHW4Z_002171"
     tRNA            complement(17415..17490)
                     /locus_tag="ACHW4Z_002171"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="ACHW4Z_002172"
     tRNA            complement(17494..17569)
                     /locus_tag="ACHW4Z_002172"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="ACHW4Z_002173"
     tRNA            complement(17621..17696)
                     /locus_tag="ACHW4Z_002173"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="ACHW4Z_002174"
     tRNA            complement(17699..17774)
                     /locus_tag="ACHW4Z_002174"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="ACHW4Z_002175"
     tRNA            complement(17910..17985)
                     /locus_tag="ACHW4Z_002175"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="ACHW4Z_002176"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="ACHW4Z_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR8881807.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="ACHW4Z_002177"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="ACHW4Z_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR8881808.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="ACHW4Z_002178"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="ACHW4Z_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR8881809.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22167)
                     /gene="tolA"
                     /locus_tag="ACHW4Z_002179"
     CDS             complement(20932..22167)
                     /gene="tolA"
                     /locus_tag="ACHW4Z_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR8881810.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKKAA
                     AEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAA
                     AAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQI
                     KSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPK
                     PPSQAVYEVFKNAPLDFKP"
     gene            complement(22232..22660)
                     /gene="tolR"
                     /locus_tag="ACHW4Z_002180"
     CDS             complement(22232..22660)
                     /gene="tolR"
                     /locus_tag="ACHW4Z_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR8881811.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22664..23356)
                     /gene="tolQ"
                     /locus_tag="ACHW4Z_002181"
     CDS             complement(22664..23356)
                     /gene="tolQ"
                     /locus_tag="ACHW4Z_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR8881812.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23353..23757)
                     /gene="ybgC"
                     /locus_tag="ACHW4Z_002182"
     CDS             complement(23353..23757)
                     /gene="ybgC"
                     /locus_tag="ACHW4Z_002182"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8881813.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23907..24200)
                     /gene="ybgE"
                     /locus_tag="ACHW4Z_002183"
     CDS             complement(23907..24200)
                     /gene="ybgE"
                     /locus_tag="ACHW4Z_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR8881814.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24200..24313)
                     /gene="cydX"
                     /locus_tag="ACHW4Z_002184"
     CDS             complement(24200..24313)
                     /gene="cydX"
                     /locus_tag="ACHW4Z_002184"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR8881815.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24328..25467)
                     /gene="cydB"
                     /locus_tag="ACHW4Z_002185"
     CDS             complement(24328..25467)
                     /gene="cydB"
                     /locus_tag="ACHW4Z_002185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8881816.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25483..27051)
                     /gene="cydA"
                     /locus_tag="ACHW4Z_002186"
     CDS             complement(25483..27051)
                     /gene="cydA"
                     /locus_tag="ACHW4Z_002186"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR8881817.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6904 a         7260 c         6705 g         6530 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcaaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gagcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttttttctt cgcgtctgca gccgctttct tcgctgcttc
    21721 ttccgcagct ttagcatctg ccgccgcttt cgctgccgcc acttcagctt gcttctgctt
    21781 taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgctcctgag ccgctaaccg
    21841 ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc
    21901 agcagcctgc tgttccttca tcttgcgctg ttcatcagaa cgcttcgcgc ttgattcctg
    21961 gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat
    22021 ggacgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg aactccagat
    22081 cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt
    22141 gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc
    22201 gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg
    22261 atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat
    22321 cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt
    22381 ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt
    22441 actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct
    22501 gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg
    22561 ccataaagat cagcaacagc accagcagta cgtccagcaa cggaacaatg ttgatttcgg
    22621 acttgagatc gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct
    22681 cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt
    22741 aattcagttc cagtttgttt acgcgctggt tgagacggtt ataagccatt accgccggaa
    22801 ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc tgcgataccg ggcgcaacca
    22861 tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga
    22921 ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt
    22981 gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc gacgcccctt
    23041 ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac tctttgaacc
    23101 cgctgtaaaa gatttgttcc gaaccagtca gattatcccg tttcccctga ctctcttgat
    23161 agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcacgcg
    23221 ccgctgcgtt aagaatacgg gttcgctgga taataatggc ccaggatgcg attgaaaaac
    23281 caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga
    23341 tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg cacgaggctt
    23401 cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc
    23461 ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt
    23521 ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg
    23581 taccacaaag gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat gatgacgcag
    23641 catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc
    23701 ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt
    23761 acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat
    23821 tgaggtttgg gaatgagggc aagttaaggg cgcgtaaaaa tttatgggcc tagagcggcc
    23881 cataattttt taaagagaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga
    23941 acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag
    24001 cccacgccgt gaatcacacc agcacagacg gcccacatca gcaataaacc atgccagatt
    24061 tccagttcac tggtcttcgc ggcaaaacga gatgggtccc aaaacataca tcctgctaac
    24121 agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaag
    24181 gtcgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt
    24241 caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg
    24301 cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc
    24361 ttctttggtg atacgaccga acattttcca gtaacaccag gcggtgtaga gcagaatgat
    24421 cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc tggaagttgc
    24481 atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat
    24541 tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca
    24601 cgcggcttta tccatacgtg cagtcaggat ggtcagcagc agcagaacca cacccagtgc
    24661 cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg
    24721 aaccacttct ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg atttcacgac
    24781 ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaaacaga ccagtgtcac
    24841 cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat
    24901 ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc ctgccagcag
    24961 gccgaacggg ttaagcaact ggaagaagtt accggtgtag tacagacgca gatattcatc
    25021 aacgttgaac ggtacgccct gcaacaggtt accgaacgct acaccaatta ccagcggcgg
    25081 aacgaagcta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat
    25141 cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat
    25201 cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag caaagagtgc
    25261 gccgcccgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa tggagttaat
    25321 cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat
    25381 gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat
    25441 aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt
    25501 agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg
    25561 tgcaaacttg aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat
    25621 cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gaattcgcca cagctgtcgg
    25681 cagcacttca ccgatagccc acggttggcg gccatattca gccacgaacc agcccgcttc
    25741 tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagaagcc attttttctc
    25801 gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca gcagcaggaa
    25861 gccacacgcc accatgatac ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc
    25921 tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt
    25981 cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac gaaccgtctg
    26041 gtcggtagaa ccagaacgca gttgttcgag cagagagtac gccttcatcc cgttacgaat
    26101 gcgttcttca tgctgcacca tcagctcttt caggccgata accggggtat ccacggaacg
    26161 cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca aatttgttcg tctcctcttc
    26221 ctgatcagga atgccgaaca aagtaaaggc agcaggcgca ggttgcgttt cccactcggc
    26281 ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg attcatcacc
    26341 cagaacaata acagacagaa cagcagccat accgaagctg gcagcgatag caaaggagcg
    26401 tttagcgaag gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa
    26461 catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg
    26521 gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc
    26581 ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt
    26641 tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca
    26701 accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat
    26761 tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc
    26821 gaactggaac tccatggtca gaccggtagc cacacccaga gcgaagttga taccaaacaa
    26881 cttgccccag aacttggtca tatctttata aatctgtttg ccggagagga cgtagaccgt
    26941 ttccataatg gccaggagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta
    27001 catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc
    27061 ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc
    27121 caatagaaat ttgtttgaac actttcatca taccatgtga atgcacacaa aaacggcgta
    27181 aagaaaggtt gcaaacatgt taataaaaac tcaaattgat cccacgtata tattacgccg
    27241 caaaatcctt acaataaaca ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc
    27301 aatttatagc taaattgccg cctttcagcc aatttgatcg agaacaattt atctcttttt
    27361 gatgcccatt tccaagactt atacattgat aaatatcaa
//
LOCUS       DAZSNG010000049        26564 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZSNG010000049 DAZSNG010000000
VERSION     DAZSNG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26564
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.050"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHW4Z_002187"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHW4Z_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8881818.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHW4Z_002188"
     CDS             348..641
                     /locus_tag="ACHW4Z_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR8881819.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHW4Z_002189"
     CDS             713..1372
                     /locus_tag="ACHW4Z_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR8881820.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHW4Z_002190"
     CDS             1464..1910
                     /locus_tag="ACHW4Z_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR8881821.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHW4Z_002191"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHW4Z_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="ACHW4Z_002192"
     CDS             3390..3809
                     /locus_tag="ACHW4Z_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR8881822.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="ACHW4Z_002193"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="ACHW4Z_002193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR8881823.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHW4Z_002194"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHW4Z_002194"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR8881824.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHW4Z_002195"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHW4Z_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR8881825.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="ACHW4Z_002196"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="ACHW4Z_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR8881826.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="ACHW4Z_002197"
     CDS             complement(8332..9864)
                     /locus_tag="ACHW4Z_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR8881827.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="ACHW4Z_002198"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="ACHW4Z_002198"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR8881828.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="ACHW4Z_002199"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="ACHW4Z_002199"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR8881829.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="ACHW4Z_002200"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="ACHW4Z_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="ACHW4Z_002201"
     CDS             complement(14765..16501)
                     /locus_tag="ACHW4Z_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR8881830.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHW4Z_002202"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHW4Z_002202"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR8881831.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="ACHW4Z_002203"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="ACHW4Z_002203"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR8881832.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="ACHW4Z_002204"
     CDS             complement(18223..18957)
                     /locus_tag="ACHW4Z_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR8881833.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="ACHW4Z_002205"
     CDS             19158..19412
                     /locus_tag="ACHW4Z_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR8881834.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..21416)
                     /locus_tag="ACHW4Z_002206"
                     /pseudo
     CDS             complement(19462..21416)
                     /locus_tag="ACHW4Z_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHW4Z_002207"
     CDS             complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHW4Z_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR8881835.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="ACHW4Z_002208"
     CDS             complement(22296..23105)
                     /locus_tag="ACHW4Z_002208"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8881836.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="ACHW4Z_002209"
     CDS             complement(23115..23873)
                     /locus_tag="ACHW4Z_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8881837.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="ACHW4Z_002210"
     CDS             complement(23870..24850)
                     /locus_tag="ACHW4Z_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR8881838.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="ACHW4Z_002211"
     CDS             complement(24850..25872)
                     /locus_tag="ACHW4Z_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR8881839.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26222..>26564
                     /locus_tag="ACHW4Z_002212"
     CDS             26222..>26564
                     /locus_tag="ACHW4Z_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8881840.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6674 a         6471 c         6855 g         6564 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaatttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat tatttaaata tgaatgacat tattgttttt ttatcgtcca
    26101 cggttacgcg acgttgaagt tacttgtttt ataatgagcg atttttgata tctcgccgag
    26161 aaaattatca gcattctctc gtttcagtgt cgtgaatata ctgttaacgg aggtgaaaag
    26221 aatgctgggg gttatacaaa acctatgttg ttgtcgtccg gattcaccgc ttgctgatag
    26281 taagcagcgt aaccagaagt taccgcaaca agactttgag gattttaaga acgacgttat
    26341 taatgtcatt aacaacaatg aagatattta ttttcttaga gagagtaatt tactgaatgc
    26401 gtttattcgg acgtgcaaaa aaagagatga taacagttac ggaaatatga tatttacgct
    26461 ttattttgat aacgatatct atgggaacag aattatcaaa gcatcgctga acggcggaga
    26521 gttgcagtta ttaagcacag ggtgtagcaa tgataacatt ctcc
//
LOCUS       DAZSNG010000050        25659 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZSNG010000050 DAZSNG010000000
VERSION     DAZSNG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.051"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHW4Z_002213"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHW4Z_002213"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR8881841.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHW4Z_002214"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHW4Z_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR8881842.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHW4Z_002215"
     CDS             2630..3391
                     /locus_tag="ACHW4Z_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR8881843.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHW4Z_002216"
     CDS             complement(3442..4326)
                     /locus_tag="ACHW4Z_002216"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR8881844.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHW4Z_002217"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHW4Z_002217"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR8881845.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHW4Z_002218"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHW4Z_002218"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR8881846.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHW4Z_002219"
     CDS             6202..6474
                     /locus_tag="ACHW4Z_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR8881847.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHW4Z_002220"
     CDS             6844..7920
                     /locus_tag="ACHW4Z_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8881848.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHW4Z_002221"
     CDS             7947..9326
                     /locus_tag="ACHW4Z_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881849.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHW4Z_002222"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHW4Z_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHW4Z_002223"
     CDS             10409..11245
                     /locus_tag="ACHW4Z_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR8881850.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHW4Z_002224"
     CDS             11250..12197
                     /locus_tag="ACHW4Z_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8881851.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHW4Z_002225"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHW4Z_002225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR8881852.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHW4Z_002226"
     CDS             13324..14082
                     /locus_tag="ACHW4Z_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8881853.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHW4Z_002227"
     CDS             14200..15558
                     /locus_tag="ACHW4Z_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8881854.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHW4Z_002228"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHW4Z_002228"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR8881855.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHW4Z_002229"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHW4Z_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR8881856.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHW4Z_002230"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHW4Z_002230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR8881857.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHW4Z_002231"
     CDS             19503..20054
                     /locus_tag="ACHW4Z_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR8881858.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHW4Z_002232"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHW4Z_002232"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR8881859.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHW4Z_002233"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHW4Z_002233"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR8881860.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHW4Z_002234"
     CDS             23307..24347
                     /locus_tag="ACHW4Z_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8881861.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHW4Z_002235"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHW4Z_002235"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR8881862.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6625 g         6546 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZSNG010000051        24961 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZSNG010000051 DAZSNG010000000
VERSION     DAZSNG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.052"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            125..2353
                     /gene="crfC"
                     /locus_tag="ACHW4Z_002236"
     CDS             125..2353
                     /gene="crfC"
                     /locus_tag="ACHW4Z_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR8881863.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2350..3228
                     /locus_tag="ACHW4Z_002237"
     CDS             2350..3228
                     /locus_tag="ACHW4Z_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR8881864.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFDDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3492..4994
                     /gene="proP"
                     /locus_tag="ACHW4Z_002238"
     CDS             3492..4994
                     /gene="proP"
                     /locus_tag="ACHW4Z_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR8881865.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5106..5195
                     /gene="pmrR"
                     /locus_tag="ACHW4Z_002239"
     CDS             5106..5195
                     /gene="pmrR"
                     /locus_tag="ACHW4Z_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR8881866.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5171..6271)
                     /gene="pmrB"
                     /locus_tag="ACHW4Z_002240"
     CDS             complement(5171..6271)
                     /gene="pmrB"
                     /locus_tag="ACHW4Z_002240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR8881867.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6272..6940)
                     /gene="basR"
                     /locus_tag="ACHW4Z_002241"
     CDS             complement(6272..6940)
                     /gene="basR"
                     /locus_tag="ACHW4Z_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR8881868.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6937..8580)
                     /gene="eptA"
                     /locus_tag="ACHW4Z_002242"
     CDS             complement(6937..8580)
                     /gene="eptA"
                     /locus_tag="ACHW4Z_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR8881869.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8684..10021)
                     /gene="adiC"
                     /locus_tag="ACHW4Z_002243"
     CDS             complement(8684..10021)
                     /gene="adiC"
                     /locus_tag="ACHW4Z_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR8881870.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10158..10919)
                     /gene="adiY"
                     /locus_tag="ACHW4Z_002244"
     CDS             complement(10158..10919)
                     /gene="adiY"
                     /locus_tag="ACHW4Z_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR8881871.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11244..13511)
                     /gene="adiA"
                     /locus_tag="ACHW4Z_002245"
     CDS             complement(11244..13511)
                     /gene="adiA"
                     /locus_tag="ACHW4Z_002245"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR8881872.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13710..14618)
                     /gene="melR"
                     /locus_tag="ACHW4Z_002246"
     CDS             complement(13710..14618)
                     /gene="melR"
                     /locus_tag="ACHW4Z_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR8881873.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14901..16242
                     /gene="melA"
                     /locus_tag="ACHW4Z_002247"
                     /pseudo
     CDS             14901..16242
                     /gene="melA"
                     /locus_tag="ACHW4Z_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            16345..17766
                     /gene="melB"
                     /locus_tag="ACHW4Z_002248"
     CDS             16345..17766
                     /gene="melB"
                     /locus_tag="ACHW4Z_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR8881874.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17905..18534)
                     /locus_tag="ACHW4Z_002249"
     CDS             complement(17905..18534)
                     /locus_tag="ACHW4Z_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR8881875.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(18657..20303)
                     /gene="fumB"
                     /locus_tag="ACHW4Z_002250"
     CDS             complement(18657..20303)
                     /gene="fumB"
                     /locus_tag="ACHW4Z_002250"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8881876.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(20381..21721)
                     /gene="dcuB"
                     /locus_tag="ACHW4Z_002251"
     CDS             complement(20381..21721)
                     /gene="dcuB"
                     /locus_tag="ACHW4Z_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR8881877.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22292..23011)
                     /gene="dcuR"
                     /locus_tag="ACHW4Z_002252"
     CDS             complement(22292..23011)
                     /gene="dcuR"
                     /locus_tag="ACHW4Z_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR8881878.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(23008..24639)
                     /locus_tag="ACHW4Z_002253"
     CDS             complement(23008..24639)
                     /locus_tag="ACHW4Z_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR8881879.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24820..>24961
                     /gene="yjdI"
                     /locus_tag="ACHW4Z_002254"
     CDS             24820..>24961
                     /gene="yjdI"
                     /locus_tag="ACHW4Z_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR8881880.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         6022 a         6066 c         6471 g         6402 t
ORIGIN      
        1 gataaaaaaa caacacacta aatataaaat tatttcttgc agtaattata aaagccgaaa
       61 acagaactca aaaacaatct ggctaaataa aagtaacaaa atttgcttta aggaagaatt
      121 ttctatgtac acacagaccc tgtatgagtt aagtcaggag gctgaacgcc tgttacagct
      181 ttctcgccaa cagttgcagt tactggaaaa aatgcctctc tctgtacccg gagacgacgc
      241 gccacaactg gctttaccct ggagtcagcc taatatcgcc gaacgtcacg cgatgctgaa
      301 taatgagttg tgtaaaattt cccgactgga aatggtgctg gcaattgtcg gtaccatgaa
      361 agcagggaaa tcaaccacca ttaatgccat tgttggtacg gaggttctgc ctaatcgtaa
      421 tcgcccaatg actgcgctgc cgacgcttat tcgccatacg cccgggcaaa aggaaccggt
      481 actgcatttt tcacatgtcg cgccaatcga ttgtttaatt caacaattac aacagcgcct
      541 gcgtgattgc gatattaagc atctgaccga tgtgctggaa atagataaag atatgcgtgc
      601 gcttatgcag cggatcgaaa atggcgtcgc tttcgaaaaa tattatctgg gtgcccagcc
      661 tatttttcat tgtctgaaaa gtttgaatga tttagtgcga ctggcgaagg cgctggacgt
      721 cgattttcct ttttctgctt acgccgccat tgagcatatt cccgtgattg aagtggagtt
      781 tgtccatctg gcggggctgg aaagttatcc cggtcagttg acgttactgg atacccccgg
      841 gccaaatgaa gccgggcaac cgcatctgca aaaaatgctt aaccagcagc tggcacgcgc
      901 ctcggcggta ctggcggtgc tggattatac gcaactgaaa tcgatctccg atgaagaggt
      961 ccgtgaggcg attttggcgg tggggcaatc ggtgccgctg tatgtgctgg tcaataagtt
     1021 cgatcaacag gatcgtaaca gtgacgacgc cgaccaggtg cgggcactga tttccgggac
     1081 gctgatgaaa ggctgtatta cgccacagca gatattcccg gtgtcgtcga tgtggggcta
     1141 cctggcgaat cgggcgcgct atgagttagc caacaacggt aagttaccag cgccagagca
     1201 acaacgctgg gtggaagatt ttgcccatgc cgcgctcggc aggcgctggc gtcatgccga
     1261 tctggcggac ctcgaacata ttcgtcatgc tgccgatcag ttgtgggaag attcgctgtt
     1321 cgcccagcca attcaggcgt tgcttcatgc cgcttacgct aacgcctcat tgtatgctct
     1381 gcgatctgct gcgcataaac tgttgaatta cgcgcagcag gcgcgggaat acctggattt
     1441 tcgtgcgcac gggttaaacg tcgcttgtga acaattgcgg caaaatatcc atcaggtcga
     1501 agaaagtttg cagctattgc aactcaatca ggcgcaggtg agcggcgaga ttaaacatga
     1561 aatcgagctg gccctgacct ccgccaacca ctttctgcgt caacagcaag atgcggtgaa
     1621 tgcgcagtta gccgccttgt ttcaggatga ttcggagccg ttaagcgaga ttcgtacccg
     1681 ctgtgagaca ctgttacaga cggcgcagaa caccatcagt cgcgacttta cgctgcgttt
     1741 tgccgagatg gaatccaccc tttgccgggt gttaaccgat gttattcgcc ccattgagca
     1801 acaagtcaaa atggaattga gcgagtcagg gtttcgtcct gggtttcatt ttcctgtttt
     1861 tcacggcgta gttccccact tcaacactcg ccagctgttc agtgaagtca tttcgcgcca
     1921 ggaagcaacg gacgagcaga gcacgcgttt aggcgttgtg cgtgagactt tttcgcgctg
     1981 gttgaatcag cccgactggg gacggggaaa tgagaaatcc ccgacagaaa cggttgatta
     2041 cagtgtgttg caacgagcat taagcgcaga agtcgatctc tattgccaac aaatggctaa
     2101 agttctggca gagcaggtcg atgaatctgt tacggcaggc atgaatactt ttttcgctga
     2161 gttcgcttca tgtttgacag aattacagac gcgtttacgc gaaagtctgg ctctgcgtca
     2221 acaaaatgaa tcggtggtca ggctgatgca gcagcaattg cagcagactg tgatgactca
     2281 cagctggatt tacaccgacg ctcagctgtt acgcgatgat attcaaacac ttttcacggc
     2341 agaacgatat tgaccaagac gttactggac ggccccggtc gcgtgctgga gtcggttcat
     2401 ccgcgctttt tagtggatct ggcgcagggc gatgatgccc gccatcctca agcccatcag
     2461 cagcagtttc gtgaacgact gatgcaggaa cttctttcgc gtgtgcagct tcagacatgg
     2521 acgaacggcg gcatgttaaa tgcgccgctt agcctgcgac tgacaatggt ggaaaaactg
     2581 gcgtcgatgc tggatcccgg tcatctggcg ctgacgcaga tcgcgcagca tctggcgctg
     2641 ctgcaaaaaa tggatcaccg ccagcactct gctttcccgg agctccccca gcaaattgcc
     2701 gccttgtatg agtggttttc agcccgttgt cgctggaagg aaaaggcgtt aacgcaacga
     2761 ggcctactgg tgcaggcagg tgatcagagc gagcaaattt ttacccgctg gcgtgctggg
     2821 gcgtataacg cctggtcgtt gcctgggcgc tgttttatcg ttctggagga gttgcgctgg
     2881 ggtgcatttg acgatgcctg ccgtctggga agcccgcaag cggtggtgtt gttgctgggt
     2941 gatttgcgcg agaaagcgac acaacatctg gcagagagta tcaatgcggc accgactacg
     3001 cgtcactatt accatcagtg gtttgcctct tcgaccgttc cgacgggcgg ggatcatgct
     3061 gattttttaa gttggctggg aaagtggacc acggcagata aacaacccgt ttgctggtca
     3121 gtgacccaac gctggcaaac tgtcgcgctg gggatgccac gactctgttc agcgcagcgt
     3181 ttggtggggg caatggtcga ggaaatcttc tctgtaaatt tggcgtaaat aatcagttac
     3241 atcaatgagt cctaaacgaa atccatgtgt gaagttgatc acaaatttaa acactggtag
     3301 ggtaaaaagg tcattaactg cccaattcag gcgtcaactg gtttgattgt acattcctta
     3361 accggagggt gtaagcaaac ccgctacgct tgttacagag attgcatcct gcaattcccg
     3421 ctcccctttt gcggccgtcg cgctgatttt tctggcgttt gcggaaatgg gccaactctg
     3481 cgaggaaagc tatgctgaaa aggaaaaaag taaaaccgat tacccttcgt gatgtcacca
     3541 ttattgatga cggtaaactg cgtaaagcca ttaccgcagc atcactgggt aatgcaatgg
     3601 aatggtttga ttttggtgtt tatggttttg ttgcttacgc attaggtaaa gtttttttcc
     3661 cgggggctga ccccagcgtg cagatggttg ctgcacttgc cactttctcc gttccctttc
     3721 tgattcgacc gcttggcgga ctcttctttg gtatgttggg cgataaatat ggtcgccaga
     3781 agatcctcgc tatcactatt gtgattatgt cgatcagtac gttctgtatt ggcttaatac
     3841 cgtcctacga cacgattggt atttgggcac cgattctgct gttgatctgt aagatggcac
     3901 aaggtttctc ggtcggtggt gaatataccg gggcgtcgat atttgttgcg gaatactccc
     3961 ctgaccgtaa acgtggcttt atgggcagct ggctggactt cggttctatt gccgggtttg
     4021 tgctgggtgc gggcgtggtg gtgttaattt cgaccattgt cggcgaagcg aacttcctcg
     4081 actggggctg gcgtattccg ttctttattg ctctgccgtt agggattatc gggctttacc
     4141 tgcgccatgc gctggaagaa actccggcgt tccagcagca tgttgataaa ctggaacagg
     4201 gcgaccgcga aggtttgcag gatggcccga aagtctcgtt taaagagatt gccactaaat
     4261 actggcgcag cctgttgaca tgtattggtc tggtaattgc caccaacgtg acttactaca
     4321 tgttgctgac ctatatgccg agttatttgt cgcataacct gcattactcc gaagaccacg
     4381 gggtgctgat tattatcgcc attatgatcg gtatgctgtt tgtccagccg gtgatgggct
     4441 tgctgagtga ccgttttggc cgtcgtccgt ttgtgctact tggtagtgtt gcactgtttg
     4501 tgttggcgat cccggcgttt attctgatta acagtaacgt catcggcctg atttttgccg
     4561 ggttactgat gctggcggtg atccttaact gctttatggg cgttatggct tctaccttgc
     4621 cagcgatgtt cccgacgcat atccgttaca gcgcgctggc ggcggcattt aatatttcgg
     4681 tgctggttgc cggtctgacg ccaacactgg cggcctggct ggtcgaaagc tcgcagaatc
     4741 tgatgatgcc tgcctattac ctgatggtag tggcggtgat tggtttaatc accggcgtaa
     4801 ccatgaaaga gacggcaaat cgtccgttga aaggtgcgac accggcggcg tcagatatac
     4861 aggaagcgaa ggaaattctc gtcgagcatt acgataatat cgagcagaaa atcgataata
     4921 ttgaccacga gattgccgat ttgcaggcga aacgtacccg cctggtgcag caacatccgc
     4981 gaattgatga ataagctgaa acggatggcc tgatgtgacg ctgtcttatc aggccaattg
     5041 aactcttaag gttcacttaa tctctgacgc gcatactctc ctccaggtta acggaggaga
     5101 gtgcaatgaa aaaccgtgtt tatgaaagtt taactaccgt gttcagcgtg ctggtggtca
     5161 gcagctttct ttatatctgg tttgccacgt tctgatcttt cttcagccgt acccaggccc
     5221 gcgtgccgga agtctcttgc cggttttgca ggaaaaactg cccgtgatgc aactgtgtaa
     5281 tgcggctgac aatacttaac cccagaccaa tcccgccata acggctgtcc atacgtacaa
     5341 acgctttact caactccccg catttactct catcaatacc tggtccttca tcttcaactg
     5401 ccatgaccgc tccgccatct tcttgcagct taatcataat gttgctgcct tgcgggctgt
     5461 aacgatgggc gttttctacc aggtttcgca ataacatccg cagcagggtt gcatcaccct
     5521 gaacggtgat gtcggcggcg ctctctggca atagcagggt ttgctgtcgc tggtcgagca
     5581 tggtactgag ttcgtcatac gaggggagaa tgacatcttc cagcagtttt acatgttgat
     5641 aattaccgga agaaaatgac tgtccggcac gcgccagttg cagcagctgg gagacgctct
     5701 ccatcatctg atcaagccgt gccactaacg gtgctacatc aatgtgatgc gttttcgcca
     5761 gcagttccag atgcaaacgc acccccgcca gtggcgttcg cagttcgtgc gcgacgtcag
     5821 cggtaaacaa cctttcgtta tccagcgtgc tggtcaggcg actgaccaga tcgttcaacg
     5881 ccgaaaccac cgcttcgatt tcgagggtgg cgctgtgaat ggcaatgggc gttaagttgt
     5941 cggcggtgcg cgcttccagc tctttttgca gctccgccag cgggcgggtg atgcggcgta
     6001 ccgcctgata gcagataaat agcgtcaggc tgaccataaa gacgccgggg acaatcaggc
     6061 tggcgaccgc ctcgcggatc tcacgcatga tgtggcgatc gttgttgcga ttgtcgcgta
     6121 gcgcctgctc aaacagctga atctgctcgg tactttcatg ccatagccag aagacgctga
     6181 tcagctcaaa caccaacaaa atggccccga tggtcaatat cagccgttgg cgcagcgata
     6241 taggtcggcg cagaaaacgc atcagattca attagttttc ctcattcgcg accagcatat
     6301 agccaaagcc gcgcacggtg cggatacggg ctttgcccac tttgtcgcgc agattgtgga
     6361 tatgcacttc cagggtgttg gtcgagggtt cattgtccca gttatagatg tcgttgtaga
     6421 gaatttcccg atgtaccgga ctgcctgctt tgagcattaa ccgtgacagc agagcatatt
     6481 ctttgggcgt cagaatcaac tcttcaccgc ccatccatac ctgacggcga cccatattca
     6541 gcgtcagatt gccaacaatc agctcacttt cgccctgatt attatggcgt cgtagcaggg
     6601 cgcggatacg ggcgtgtaac tcttccagcg caaaaggctt caccagatag tcatcggcac
     6661 cgacatccag cccggcgatt ttgtcggtca gcgtatcgcg agcggtgagg atcagtaccg
     6721 gcagggtgta ttttttctgc cggatacggg cgagaaaatg cagtccatct tcgtcgggta
     6781 accctaaatc cagtaccacc aggctgtaat gaccgtcctc aaggctttgt tccgccatcc
     6841 gcgcggttgt cacgccatcg cacgcgtagc cttcggtttg cgccgccaga atcagtccct
     6901 gcaataacag cgtatcgtct tcaacaatca gaattttcat ttactcactc tcctgcaagt
     6961 ttgcagaata tcatccgcgg cctggtaata cttcgtctca acgccagtta atcccaatag
     7021 cgtggagaat aaattgtctt gtgaatagtg ttgcgtttgc gcctgttttt gcaggcagtt
     7081 ctggtcaacc tgataccgct tttgataatc ctccgacagc cacagcagca ttggcacctg
     7141 tttttggcta tccggggcga tggcataagg cagaccgtgc agatagatgc cattttcacc
     7201 taacgattca ccgtggtcag aaagataaac caggctggtg gtaaatttat cctgatgttc
     7261 tttaagcaga ttaatcgctt tatcaacaat atagtcgacg taaaccagcg tgttgtcgta
     7321 agtgttcacc agttgctctt tggtacaggt ctggatctca ttggtgtcgc aggttggggt
     7381 aaatttcctg aactgcggcg gatagcggtt gtaataggtc ggaccgtggc tgccgatggt
     7441 gtgtaagaca atcacgccat caccctgcag gttattgatg tactcttcca gcccgtggaa
     7501 cagcacttcg tcatagcatt cgccgttgat gcactgatca ggcagattca gcgcggtgac
     7561 gttctggtga ggcacgcggt cgcaggcacc tttacagccg ccatcgttgt cattccacag
     7621 cacgttgatg cccgctcgct gaatgatatc cagcacgcct tcctggtgct gtgccagctc
     7681 ttctttgtag tgctcacgcg gcatatccga gaacatgcac ggtactgaaa ctgccgttgc
     7741 cgtgccgcaa gatgcggtat tagggaaata gaccacgtta tctttcgcca gccgcgggtt
     7801 agtttcacgc gggtagccgt tgagggagaa gttctccgcc cgcgaggttt cgccgacaat
     7861 caggatggtc aaatttttac gtttttcgtt ctgcattaac gggttgcggt gcgcgtcttc
     7921 accaattcgc accagcggca gatttgccag tcgctgatgg gagtaccatg accagctggc
     7981 aacaatgctg ttagaggggc ttaaggattt caccagctct ttgttattgc ggaacaacga
     8041 ggcgtagtct ttataaaaca gtgcggcgac cagcaaaatc agcagtacag aaaccagaat
     8101 attggctcca cggaaaagaa cactgcgcag acgcgaggtg gcaggtttga tttttagcca
     8161 gcaggcaatc agctcagcaa gcacgccgct gaatcccagc gttaataaca tttgcggtgt
     8221 catcagcgca taactttctg ctggagtggt atcaataata ttggcaatca tcgagcggtc
     8281 gatgacgatg ccgtaagtca ttatgaaata ttgtgcagcc gcgccaatca gaataaacag
     8341 gcaggccagt ggtcgattaa gccataagaa agagcttagt gtcaggacaa tattaatcac
     8401 gctgaaagcg acaaccggca tcgacaagaa aaccagtacg ttatgcagcg aatccagcgg
     8461 cagcgcctgc aacacctgtt taaaaaaggc aatattcagg cagatagaga tataaaaagc
     8521 ggccaacaat agccaggcga gtaaattcaa agagggtctt tttagtaggc gcttcaacat
     8581 cacggtgttt ccatcgaaca aagtgcgcat atgctcgcaa agcaaaatta agccaacctt
     8641 aagttcttaa ggttggcttt tatgtttgct ggattaaggg gaattaatct ttgcttattg
     8701 gtgcatctaa gggatacggg tttttatgta gccggttgta attcagcgca tacatggcgg
     8761 tgatgaccat cagggtgaca aatgaccaca taacctcttt cgccccggac cccaccacgg
     8821 cccaaatgca gtagaggaag gcaatggtag taacagccag atatgccggg cgtgctttac
     8881 caaagtgacc gtggccgagc agcagtaacg ccgcacaggt gtaaagatat ggcaccagtg
     8941 taaagatgac cgagacggaa gaaaccagac cgaactcttt ggtcgcgttt ggtgaaatgc
     9001 tgctgagctg gaagatggtc atcaaaatac cgacgataat cagccccgcc actggcgtac
     9061 ccgctttatt tacacgggca aaaatcggtg ggaacagtcc gtcatcggca gcggctttcg
     9121 ccgtttgacc cgccagcaac gtccagccgc ccagtgaacc taagcaaccc gcagctgcgc
     9181 agaaggaaac aatggcccca gcggtgtcac ccaacgccat ccgtgcggca tcaccgaatg
     9241 gcgaggcaga aacgcgcagt gcggcattag ggatcatccc cataatcgcg gtggtggaga
     9301 gcacatagca aacggcggca atcaataccc caccaatggt ggcgataggg acattgcgtt
     9361 tcgggttttt caccacacct gcggcaacgg aggcactttc cacaccgatg aacgaccaca
     9421 gcgtaacgtt aagggtactt tgaatcgcac cgaaggtgcc caggccgctg acgttccatg
     9481 ccgccatata ggtttcacca cggaaccaga accagccaaa tacggcaatc ccgacgatgg
     9541 ggatcagcgc cagcacagtg gcaacggcct gcacgcgggt gatcattttc ggaccgacaa
     9601 tgttcagcag gacgaagatc cacagcacca cgacgcaggt gatggttaat accaatggat
     9661 ctttcagaat cgggaagaag taacttaaat atcctacgcc aatgaccacc atggcgatat
     9721 tgccgatcca gcaggccagc cagtagagga cgttggtttg ataaccgaga aacgggccaa
     9781 agcagcggcg ggcgtaagcg taagaaccac caggacttgg gtcgaggaac gacattttgg
     9841 cgtataccat cgagagcccc agcgcaccga taatcgtcac caaccatcca taaatggcaa
     9901 tcccgccagt ggaggccagg tttgcaggta acagaaaaac acctgacccc ataatattcc
     9961 ccgacaccat cagggtgacg gggattaagc ccactttgtg agcatcagca tccgaagaca
    10021 taattaaact cctgcgaagg cgagcttcgt gacaataaat tacgtcatat catacgcctg
    10081 cattcgatag ttgaattatt atcgaccggg ttatcgctga tgccgttatt aatatagctc
    10141 ttcgttaagg ttaaaaatca ggcgacagtg cgttctctgt gctgactaac ataatgcagc
    10201 ggcgtcatac cgtagaagtc tttaaagaca gaaataaagt acgacgtact gttgtagccg
    10261 caggactgtg atacctgaga gatattttta ccgtccatca ttaattcatt tacggcataa
    10321 cgcatgcggc aggtggtgat tatttggcta taactggtgt tttcgctttt caactttttc
    10381 tttaacagac ttgggctaag acataaacaa ctggctacca tactaagatt ccagtctttg
    10441 tgaatatcgc tttcaataat ttgataaacg ctgtcactta cacaattacg taacatatac
    10501 atcattaatg aaaggaattt tttactgtcg agaaaacggg atagcaccgt gaataataat
    10561 gcgcgtgttc gttccttttc tgactcagtt tctgcgggca tcatgctgtg ttgcgcggca
    10621 acgcgaaaga cgtctggcgt caggcatggc agggtgagta tgggcgtagc gctacgttgc
    10681 catactggta tttgcgagag atctttattc agaaaccgga ggtaatcttt gatgatgtcg
    10741 tgactaatat gagcaaccaa agtgttgtta agagaggaga cgtcgataat attattttca
    10801 caatttaata acgccatatg gttagcttta aggctaatag gttctttccc attcaccctt
    10861 atccagacat ctttttcagt caataaaaca atacaaggtt ggtcgctgca aatcctcata
    10921 catgtactcc tgagtgcgaa taaaagacca gttctcgcta aagcaaagcg ataccgggaa
    10981 atggttatat aattgcatga tgaatataaa catgcataaa aataacgagt tttactaata
    11041 ttacaaattt ttatagtaat ttctcatgta catatttttt tacaggataa ataacaggca
    11101 aaaaaaagcg tggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt
    11161 atgaaaacgt tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa
    11221 ttacgccttc agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca
    11281 ataatttcag tcccttcagt ttcgtgttca aatctaggga aatggtggtc ccaggattgc
    11341 agcgagcgta aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc
    11401 agcatcggga ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga
    11461 ttttcaatgg ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg
    11521 gtgatttccg ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt
    11581 tctttcagcc aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg
    11641 tcaggatatt gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag
    11701 tggtgtttga aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc
    11761 atagagaaca ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga
    11821 ccaagccagg cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca
    11881 cccattcccg gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga
    11941 atatctttga agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg
    12001 gtcagcagtt tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg
    12061 acgacttctt tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat
    12121 agccgcgcca tcgcctgacg gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac
    12181 aggccgctgt tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac
    12241 agcggggagg tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc
    12301 ccacgacctt cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg
    12361 atggagtggg tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca
    12421 tagtgatcgg catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc
    12481 agacgatcgg aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg
    12541 tcataggtgc agttggttac cacgcagtaa gacggttttt gcccggcttt gtctttggtc
    12601 agcgggcttt cactgatttt cttttgcaag gtttcaggtt gcatttcctg cggatagatt
    12661 ggcccgataa tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc
    12721 agcatcaaac cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta
    12781 tcggtcatgc aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac
    12841 caggagcgat cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca
    12901 gtatggtcaa gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt acggaacaga
    12961 ttttcaccat agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg
    13021 ccctggtggc ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg
    13081 ctgaacagtg gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg
    13141 gcgataaagt cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga
    13201 tcccggtcca ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt
    13261 tgttggcgct catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgc
    13321 tccatttgat agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa
    13381 ccatcatcaa aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc
    13441 agacgctcaa cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt
    13501 aatactttca tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc
    13561 cggccttttc gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat
    13621 aaacaatacg ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac
    13681 tatttttttg gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg
    13741 ctaagtttgc ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa
    13801 cgactacttg aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg
    13861 ctcagtaacg cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat
    13921 tgcatgaccc gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc
    13981 acatcgttga tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta
    14041 acataaaatt gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc
    14101 agaatcggtt cccagctaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg
    14161 gcgagctggc gaatttgctc attcggactg tttaattcct gctgccagcg gcgtacttca
    14221 aacgggctaa gttgctgtgt ggcaagtgat ttgatcacca tgccgtgagt gacgtggtta
    14281 atcaggtctt tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc
    14341 atgctctgac aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg
    14401 atatgaccct gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg
    14461 aaaggcacat tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg
    14521 cgaaactcaa tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt
    14581 ttttcgtcgc tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctagaat
    14641 atcagaatta tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct
    14701 cttcccagag gggcgagggg actgtccgag catcatgagg ccgaaaactc tacttttcag
    14761 gtaatttatt cccataaact cagatttact gctgcttcac gcaggatctg agtttatggg
    14821 aatgctcaac ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc
    14881 aagcaagcca ggagatctgc atgatgtctg cacccaaaat tacatttatc ggcgctggtt
    14941 cgacgatttt cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg
    15001 cgcatattgc cctgatggac attgacccca cccgcctgga agagtcgcat attgtggtgc
    15061 gtaagctgat ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag
    15121 aagccttaga ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt
    15181 gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata
    15241 cgttggggcc gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt
    15301 gcgagaaccc ccgatgccac catgctcaac tatgttaacc caatggcgat gaatacctgg
    15361 gcgatgtatg cccgctatcc gtatatcaaa caggtcgggc tgtgccattc ggtgcaggga
    15421 acggcggaag agctggcgcg tgacctcaat atcgacccgg ctacgctgcg ttaccgttgc
    15481 gcaggtatca accatatggc gttttacctg gagctggagc gcaaaaccgc cgacggcagt
    15541 tacgtgaatc tctacccgga actgctggcg gcttatgaag cagggcaggc accgaagcca
    15601 aatattcatg gtaatactcg ctgccagaat attgtgcgct acgaaatgtt caaaaagctg
    15661 ggctacttcg tcacggaatc atcagaacat tttgccgaat atacgccgtg gtttattaag
    15721 ctaggtcgtg aggatttgat tgagcgttat aaagtaccgc tggatgagta cccgaaacgc
    15781 tgcgtcgagc agctggcgaa ctggcataaa gagctggagg agtataaaaa cgcctcccgg
    15841 attgatatta aaccgtcacg ggaatatgcc agcacaatca tgaacgctat ctggactggc
    15901 gagccgagtg tgatttacgg caacgtccgt aacgatggtt tgattgataa cctgccacaa
    15961 ggatgttgcg tggaagtagc ctgtctggtt gatgctaatg gcattcagcc gaccaaagtc
    16021 ggtacgctac cttcgcatct ggccgccctg atgcaaacca acatcaacgt acagacgctg
    16081 ctgaccgaag ctattcttac ggaaaatcgc gaccgtgttt accacgccgc gatgatggac
    16141 ccgcatactg ccgccgtgct gggcattgac gaaatatatg ctcttgttga cgacctgatt
    16201 gccgcccacg gcgactggct gccaagctgg ttgcaccgtt aaaacgcgac taaacgctac
    16261 tgcgccgggg gatttattcc ggcgcacacc tctgacgata ccaataacag aaggcgggcg
    16321 ttggtaacag cgacccgata ccctatgagc atttcaatga ctacaaaact cagttatgga
    16381 tttggagcgt tcgggaagga ttttgcgatc ggcattgtgt atatgtacct catgtattac
    16441 tacaccgatg tcgtcgggct gtctgtgggt ttggtcggta ctttgtttct ggtggcgagg
    16501 atctgggatg ctattaacga tccgattatg ggatggattg taaatgctac gcgatcgcga
    16561 tggggtaagt tcaaaccctg gatcctgatc ggtacgttgg caaactctgt aatcttattt
    16621 ctcctcttta gtgcgcatct gtttgaaggt actactcaga ttgtctttgt ttgcgtgacc
    16681 tacatcctct ggggcatgac ttacaccatt atggatattc ccttctggtc gctggttcca
    16741 accatcacgc tcgataaacg tgaacgcgaa caactggttc cttatccgcg tttttttgcc
    16801 agtctggcag gctttgttac ggcaggtgtg acgctaccat ttgttaatta tgtcggcggt
    16861 ggcgatcggg gatttggctt tcagatgttc actctggtat tgatcgcctt ttttattgtt
    16921 tcaaccatca tcactctgcg caatgtgcat gaagtctttt cgtcagacaa tcaatcgtct
    16981 gctgaaggaa gccatctgac acttaaagcc atcgttgcgc ttatttataa aaacgatcag
    17041 ctttcatgcc tcttgggtat ggctcttgct tataatgtag ccagcaacat tattaccgga
    17101 tttgctatct attatttctc atatgttatc ggtgatgcgg atttgttccc ctattatctg
    17161 tcgtatgcgg gagctgctaa cctggtgacg ttagtattct tcccacgctt agttaaatca
    17221 ttatcccgac gcattttatg ggccggagca tctattcttc cggtgttaag ctgtggtgtt
    17281 ctcctgttaa tggcattaat gagctatcac aacgtcgtcc tcattgtgat tgcgggtatt
    17341 ttgctgaatg tgggaacggc gcttttctgg gtattacagg tcatcatggt ggcagatacc
    17401 gttgattacg gtgaatataa actgcacgta cgctgtgaaa gcatcgctta ctccgtgcag
    17461 actatggtgg tgaagggcgg ttcagccttt gcggcttttt tcattgcggt tgtgttaggg
    17521 atgattggct atgtaccgaa tgttgaacag tctacgcaag ccctattagg tatgcagttt
    17581 attatgattg ctctaccgac tctgtttttc atggtaacgc tgattctcta cttccgtttc
    17641 tatcgcctca atggcgacac gctgcgcagg atccagattc atctgctgga taaatatcgc
    17701 aaagtaccgc ccgagcctgt tcatgctgat attccggtcg gtgcagtgag tgatgtgaaa
    17761 gcctgacgtg gacaatagct aacgaaaatg gcctgatgcg atatgtttat caggccacgt
    17821 cttaatgtaa tgctttggat ttgcaaattt tgtagaccgg ataaggaatt cacgccgcat
    17881 ccggcatcaa caaagtgcac gttgttatcc agttatcaag ccatagcgcc gtagctggcg
    17941 gcaatgaaag cgagcgagca atatcactgt cgttaatgcg ccaagcagcg cgcagaacat
    18001 atcggattgc gtgtcccact ggtcgccctg ggtaccgaga aaatcatccg ctccctggcc
    18061 catcgccagt gccgcccacc actcgattaa ttcatacatc gcgcttatcg ccagcgccac
    18121 gcaacagacc agaaacgcca ccattttacg tccgcgcacg tacatcccgc gaacgagaat
    18181 ttctcgtgcc accagtgcag gcaccagccc ctggaaaaag tgccccagct tgtcatacgg
    18241 attacgactc aaccccagcc attcctgcac ctcaaaacca acggggactt tcgcgtaggt
    18301 gtactgcccg cctaccatca ggatgatggc gtgaaagaag atgagcgtat agagcagcag
    18361 cgttaacggg taacgtctgg cggtggcaag tagtagcggc acgacgataa tcaccggtgt
    18421 cacttccagc agccaggtga gtttgtcatg ggccgaaatg ccggtataaa tcaggattaa
    18481 cgttagcgcc agtgcgccag tgttaagaat taacggcttg agtgtgcggg tcatggtgag
    18541 ttcgcaagtc agggaaaata ctgactattc accagcaagc atgaaattac aatcgcaaaa
    18601 gaggtggccc agggggatca cctggcagca tgctgccagg cgctgggccg aagaggttac
    18661 ttagtgcagt tcgcgcactg tttgttgacg atttgctgga agaagtcgtt acctttgtca
    18721 tcgaccagga taaacgccgg gaaatcttct acttcgattt tccagatagc ttccataccc
    18781 agctccggat aagcgacgca ttccagatgc ttgatgctct gctgcgccag taccgccgcc
    18841 ggaccgccga tgctaccgag gtagaagccg ccgtgtttat gacacgcgtc ggtaacctgc
    18901 tggctgcggt tgcctttcgc cagcatgatc atgctgccgc cgtgggattg cagcagatcc
    18961 acataggagt ccatacgacc tgcggtggtt gggccaagtg aacctgatgg ataaccggca
    19021 ggggttttcg ccggacccgc gtagtagatc gggtgatctt tgatgtactg cggaagttct
    19081 ttaccggcgt caatcagctc tttcagcttg gcgtgtgcaa tatctcgacc cacaataatg
    19141 gtgccagtga gcgacaaacg agtggaaacc ggatattgcg aaagctgggc gaggatctct
    19201 ttcatcgggc ggttaaggtc gaccttcacc gcttcgcctt caccggcctg acgcagttct
    19261 tgtggaatgt actgacctag attgtgttcc agtttttcga tccagatacc ttcgcggttg
    19321 attttcgctt taatgttacg gtcagcggag caggagacgc ccatgcctac cgggcaggat
    19381 gcgccgtgac gtggcagacg gataacgcga atgtcgtgcg cgaagtattt accgccaaac
    19441 tgcgcgccaa gaccgagttt ctgggcctct tccagcagtt cctgttccag ctggacatcg
    19501 cggaacgcct gaccatgttc gttcccttcc gtcggcagtt catcgtaata gtgagcgctt
    19561 gctaatttga cggttttcag gttgttttcc gcagacgtac cgccaatcac aaacgcgata
    19621 tggtacggcg ggcaggctgc agtaccgagg gtacgcattt tctcgacgag gaagtttttc
    19681 agtttgccgg gagtcagcag ggctttggtt tcctggtaga gatacgtttt gttggcagag
    19741 ccgccgcctt tcgcaacgca aaggaatttg tactcatcgc catctaccgc gtacaggtcg
    19801 atttgcgcag gcaggttagt gccggtgttg acctctttgt acatgtccag cgccgcgttt
    19861 tgcgagtagc gcaggttatc ttcgatatag gtgttataga cgcctttcga cagcgcttct
    19921 tcatcaccgc caccggtcca cacgcgctgg ccttttttac cgacgatgat cgcggtgccg
    19981 gtatcctggc aggtcggcag cacgcctttg gcggcgattt cggagtttct taagaattgc
    20041 agtgccacgt acttgtcgtt ttcgctggct tctggatcgt gaagaatagc cgcaacctgt
    20101 ttctggtgtg ccggacgtag catgaaagaa gcgtcgtgaa aggcttgctg cgccagcagg
    20161 gtcagggctt ctggttccac tttcaggatg gtttcgccgt cgaagtcggc aacgctaacg
    20221 taatcggaag tgagtagata gtattcggta ttgtctttcc ccatcgggaa aggtgcctgg
    20281 tagataaagg gtttgtttga catagcttcc agcctgtaac tctgtatttg ttattcgaaa
    20341 atggcgtgcc gcgtagtcac gggcggcacg caaagtgcat ttataagaac ccgtacatcg
    20401 cggcgaagat ccagccgaag acgcacgata cgctcacacc aatcaacccc ggcagaataa
    20461 aactgtggtt gatgacgaag cgaccgatgt gggtggtgcc ggaacggtca aactgaatcg
    20521 ctgccagatc gctcggataa gtcggcagga tgtaataacc gtagcaagcc ggtgctgaag
    20581 ccacgatgta tgccggatca acgccgatcg ccagcgcgac cggaacaatc gccgccagcg
    20641 ccgcagcctg agagtttaca aacttggaaa ccagcagcag aacaatggca tacgcccacg
    20701 gatactcttt caccatttca cccagtacgc cctgaatttc agacatatgc gcaccgaaca
    20761 tggtttctgc catccatgcg ataccgtaca ccgccacgat ggcgatcata ccggaacgga
    20821 agacttcgtt ttttgagata gacgcgggat tggttttggt caggataata atcagcgccc
    20881 cggtcagcag cataaacatc tgaataacca gtaccatcga cagcggtttg ccgccgaagg
    20941 atggacgcag gtccgaatca gcaccaagaa gggcgactac agcgattgcc ccgaggaaaa
    21001 tccacattgc cagccagttg cttttcggca gttttttatc cagcagcgtc gcggtatcac
    21061 cgtaaacata ctcacggttt tccggtacgg agatgaattt ctggaactct tcgtctttat
    21121 ccagatcttt accgcggaac cagctgaaga taccgatcgc caggataccg attaacgtcg
    21181 atggaatggt gattgccagc agatcgagga actcaagatg gcgaccatca aaggtgacat
    21241 tacccagcat cgcaaccaga gacacgaccg caaccgacac cggactggcg ataatcccca
    21301 tctgtgcacc gatagaactt gccgccatcg gacgttccgg acggatgttg ttcttaatgg
    21361 cgacgtcgta gatgatcggc agaatggtgt aaaccacatg acccgtaccg caaagaatgg
    21421 tcagtgtaca ggtcacaaac ggcgcgacaa ttgagacata tttcgggttg cggcgcagca
    21481 gcttctcggc aatttgcagc atgacatcaa gaccgcccga agcttgcaag gtcgccgatg
    21541 ccgccaccac cgcaatgata accagcatga catcaactgg tggtttacct ggctgaaggt
    21601 ggaagacgaa gaccagaatg accagaccga taccgcctaa taaacccagc gcgataccac
    21661 cctttctggc accataaaac agacatatta gtattatgat aagttggata gtaaataaca
    21721 tgtgtgaacc ctcgcgataa tcctatttaa atttttgctg aatagatcac agtcacgttc
    21781 tgttttgtat gaactgtttt cagagcctga ctaaatatcc gtttggtcgt taccggcttt
    21841 agcaaatacc tcacagtgaa tattggctga ttaaagcagc ggcaaattta tgcactgtga
    21901 taagcggctt tttcagagag aaaaactctc tggcgatggg tttatagttt gtactgcgca
    21961 gatgttaatg ttttgtttgc tgttatctta ttgatattta tcggtctatt ttttgttctc
    22021 agggcagata tcattaggtt tatagattaa tctgatctac ccatttgtgg gtaaaaatac
    22081 acataatgcg ggtgacataa tagttaatta acttttgtta tcgttttgaa attaaaaaca
    22141 ccgttcatct gaagagatat taatttttaa cgatgatgga gggataatta tatttgatct
    22201 ggcacaagtt ttactgatta acgatgtaac ttgtgccagg ggtattttgc attacggtaa
    22261 taattattac ttacagataa ccagcgatcc gttattggca atattgtttc agcagtgagt
    22321 agtgttctgc ctgaatacgg taacggtaaa ccggacgccc cgtgacgcca taatggatac
    22381 tggtgaacaa gatgtggcag ttgaccagcc agatgaggta ttttcggcag gaaacacgcg
    22441 agatgttaac ctcgttagcc agctcgtcgg ttgaaaactc atagtcctga tgcgcgtcaa
    22501 tccactggca cagcgtgcgt aacgtctgcg gcgttaagcc ttttggcaag cgacgaggat
    22561 cctgttcgtt ggagctgctg ccgtggatta gctgatcaag ctcggcctgg tcataatact
    22621 gatgtttttc cagcgccatt ttcttttgcc gccagccggt gagcgcctct tcaaagcggg
    22681 aagcctggaa gggtttgatc aggtaatcca cgacaccgta atgcagcgaa tctttaatgg
    22741 ttgccgcatc ggctgcggag gagatgacaa tcacatcact tttgcaacgc gcgttatgca
    22801 ggacaggcag taaatcgagc ccgttctctt tttgcatata gatatcgagc aatatcaggt
    22861 cgataggcgc atcgctattg aagataatct ctttggcttt ctccagcgtc gaggctgttc
    22921 cacagcattg aaagcctggg atttgtgcta cgtatcggcg attcagctcc gcgaccattg
    22981 cgtcgtcatc gataattaat acattgatca tctgttcgac ctctccccgt cccagggtat
    23041 ctggacaaaa aattgtgtga aaatccccgg ttccgattcc acggcgatgc tgccgccgag
    23101 attttctacc tgttgtttga caagtgctaa accgacgcct cgctcgcttc cttttgtcga
    23161 gacacctttg tcaaaaatgt gatcgatttt gtcgggtgcg atccccggtc catcatcgtt
    23221 aacttcgcag tgcagccagc cgtgacggta gtgcaatgtt acgctaattt cgccaccggg
    23281 ttccggccct aatgcttcca gcgcgttttc tatcagattt cccaatgtgg taatcagcgt
    23341 cgcgacctgg tcctcactgc cgctgtctgg cagctggctt tcactgttta aaatcagcgt
    23401 atggcctaaa tcggtcgcgc ggttaatctt gctgattaaa aaaccagcga taaccggaga
    23461 tttgatctta cccagcagag agccaatctc ttcctgatag ttattggctg ttttgagaat
    23521 gtaatcttcc aactgcttat aactcttcag atgcaataat ccgagaatca catgcaattt
    23581 attcataaat tcgtgggatc gttcacgaag tgcgtcagca tagttgacca gaccgtcgag
    23641 tcgctgcatc agtttacgaa cttcagtttt gtccctgaag gttgaaatgg caccgatgat
    23701 aacgccatta ctgcgcaccg gaacggtgtt gatcagtaat agccggtctt taatcgtaat
    23761 ctcttcgtcg cggcgcggcg taccgtcgcg taacacttcc gagacatcta ccacctgtga
    23821 ccatgagtgg ctgagcgtcg acagtttctc atcgtcctgc gacttacggt aattcagcaa
    23881 ttcttgtgcg gcatcgttga tcagcgtgac ctcgccgcga tcgtccacgg caacgacgcc
    23941 ttctttgata gactgcaaca tggcctggcg ttgctcaaac agcgtggaga tttcgtaggg
    24001 ttccaggccg aaaaggattt ttttcagtac cttaaccaga atgcaggtgc caatcagtcc
    24061 gaccagcatg ccaaataata ccgaccagat aatgctccag cgactgtcat tgatctgttg
    24121 ggtcacgcgg cttaactcaa ggccgatcgc caccacgcca atttgtttat gattttcatc
    24181 gtagatgggg gtaaatacgc gtaaagcctg cgccagaaaa ccgcgattga tagcgacatt
    24241 ttcttcgcca ttcagcgcat taaggatgtc atcaccttta aatggctgac caatacgctg
    24301 ggcttcagga tgtgagtagc gaagactttg catatcggta acgacaataa acagcagatc
    24361 gttgcgtttg cgcacggctt ccgcgatggc ctggatgcca ctctcctgcg gttttttctg
    24421 caagccctga cggatttccg gcgagtcggc gagggtacgc gccactgcca gtgccttgtt
    24481 ggccagcccg tctcgcgtca tatcactgat ttgcgagaag taaatcagat gcaccaccaa
    24541 tagcaccgag aacagtaccg cgctgaccat taagatcaca gtggtactca atttcatcgg
    24601 acgtttgcgt aatatgtggt agggcaatga atgtctcatc agcttccttg tgtgacaaat
    24661 ttcttaagca ttatctctga tgaggcgggt aattcaaagg gagtaaaaat gattggctat
    24721 ataggggaag agactctggc aacggaaact gccagtgctg tatgaagatt ccggggctat
    24781 gcttatagcg ataatcatac tgatgagaga gggaaggtca tggatcaggc gctactggac
    24841 gggggttatc gctgttatac cggcgaaaag atcgatgtct atttcaacac tgcgatatgt
    24901 cagcattctg gcaattgcgt acgtggcaac ggcaagttat ttaatctcaa acgaaagtcg
    24961 t
//
LOCUS       DAZSNG010000052        24589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZSNG010000052 DAZSNG010000000
VERSION     DAZSNG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24589
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.053"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..94)
                     /locus_tag="ACHW4Z_002255"
     tRNA            complement(2..94)
                     /locus_tag="ACHW4Z_002255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHW4Z_002256"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHW4Z_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR8881881.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHW4Z_002257"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHW4Z_002257"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR8881882.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHW4Z_002258"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHW4Z_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR8881883.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHW4Z_002259"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHW4Z_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR8881884.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHW4Z_002260"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHW4Z_002260"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR8881885.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHW4Z_002261"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHW4Z_002261"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR8881886.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="ACHW4Z_002262"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="ACHW4Z_002262"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="ACHW4Z_002263"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="ACHW4Z_002263"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR8881887.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="ACHW4Z_002264"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="ACHW4Z_002264"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR8881888.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="ACHW4Z_002265"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="ACHW4Z_002265"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR8881889.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="ACHW4Z_002266"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="ACHW4Z_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR8881890.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="ACHW4Z_002267"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="ACHW4Z_002267"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR8881891.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHW4Z_002268"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHW4Z_002268"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR8881892.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHW4Z_002269"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHW4Z_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR8881893.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="ACHW4Z_002270"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="ACHW4Z_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR8881894.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="ACHW4Z_002271"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="ACHW4Z_002271"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR8881895.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHW4Z_002272"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHW4Z_002272"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR8881896.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHW4Z_002273"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHW4Z_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR8881897.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHW4Z_002274"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHW4Z_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR8881898.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20948..21019)
                     /locus_tag="ACHW4Z_002275"
                     /pseudo
     CDS             complement(20948..21019)
                     /locus_tag="ACHW4Z_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHW4Z_002276"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHW4Z_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR8881899.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHW4Z_002277"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHW4Z_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR8881900.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24589)
                     /locus_tag="ACHW4Z_002278"
     CDS             complement(23682..>24589)
                     /locus_tag="ACHW4Z_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR8881901.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5799 a         6918 c         6307 g         5565 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgtta
//
LOCUS       DAZSNG010000053        24369 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZSNG010000053 DAZSNG010000000
VERSION     DAZSNG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24369
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.054"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            197..1279
                     /locus_tag="ACHW4Z_002279"
     CDS             197..1279
                     /locus_tag="ACHW4Z_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR8881902.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1768..2566)
                     /locus_tag="ACHW4Z_002280"
                     /pseudo
     CDS             complement(1768..2566)
                     /locus_tag="ACHW4Z_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2576..3127)
                     /gene="ybcL"
                     /locus_tag="ACHW4Z_002281"
     CDS             complement(2576..3127)
                     /gene="ybcL"
                     /locus_tag="ACHW4Z_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR8881903.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3296..3628)
                     /gene="emrE"
                     /locus_tag="ACHW4Z_002282"
     CDS             complement(3296..3628)
                     /gene="emrE"
                     /locus_tag="ACHW4Z_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR8881904.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3973..4287)
                     /gene="fliE"
                     /locus_tag="ACHW4Z_002283"
     CDS             complement(3973..4287)
                     /gene="fliE"
                     /locus_tag="ACHW4Z_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR8881905.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4501..6160
                     /gene="fliF"
                     /locus_tag="ACHW4Z_002284"
                     /pseudo
     CDS             4501..6160
                     /gene="fliF"
                     /locus_tag="ACHW4Z_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            6153..7148
                     /gene="fliG"
                     /locus_tag="ACHW4Z_002285"
     CDS             6153..7148
                     /gene="fliG"
                     /locus_tag="ACHW4Z_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR8881906.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7141..7827
                     /gene="fliH"
                     /locus_tag="ACHW4Z_002286"
     CDS             7141..7827
                     /gene="fliH"
                     /locus_tag="ACHW4Z_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR8881907.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7827..9200
                     /gene="fliI"
                     /locus_tag="ACHW4Z_002287"
     CDS             7827..9200
                     /gene="fliI"
                     /locus_tag="ACHW4Z_002287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR8881908.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9222..9563
                     /gene="fliJ"
                     /locus_tag="ACHW4Z_002288"
                     /pseudo
     CDS             9222..9563
                     /gene="fliJ"
                     /locus_tag="ACHW4Z_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            9560..10687
                     /locus_tag="ACHW4Z_002289"
     CDS             9560..10687
                     /locus_tag="ACHW4Z_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR8881909.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10792..11256
                     /gene="fliL"
                     /locus_tag="ACHW4Z_002290"
     CDS             10792..11256
                     /gene="fliL"
                     /locus_tag="ACHW4Z_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR8881910.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11261..12265
                     /gene="fliM"
                     /locus_tag="ACHW4Z_002291"
     CDS             11261..12265
                     /gene="fliM"
                     /locus_tag="ACHW4Z_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR8881911.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12262..12675
                     /gene="fliN"
                     /locus_tag="ACHW4Z_002292"
     CDS             12262..12675
                     /gene="fliN"
                     /locus_tag="ACHW4Z_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR8881912.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12678..13043
                     /gene="fliO"
                     /locus_tag="ACHW4Z_002293"
     CDS             12678..13043
                     /gene="fliO"
                     /locus_tag="ACHW4Z_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR8881913.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13043..13780
                     /gene="fliP"
                     /locus_tag="ACHW4Z_002294"
     CDS             13043..13780
                     /gene="fliP"
                     /locus_tag="ACHW4Z_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR8881914.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13790..14059
                     /gene="fliQ"
                     /locus_tag="ACHW4Z_002295"
     CDS             13790..14059
                     /gene="fliQ"
                     /locus_tag="ACHW4Z_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR8881915.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14068..14853
                     /gene="fliR"
                     /locus_tag="ACHW4Z_002296"
     CDS             14068..14853
                     /gene="fliR"
                     /locus_tag="ACHW4Z_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR8881916.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15138..15761
                     /gene="rcsA"
                     /locus_tag="ACHW4Z_002297"
     CDS             15138..15761
                     /gene="rcsA"
                     /locus_tag="ACHW4Z_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR8881917.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15805..15993)
                     /gene="dsrB"
                     /locus_tag="ACHW4Z_002298"
     CDS             complement(15805..15993)
                     /gene="dsrB"
                     /locus_tag="ACHW4Z_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR8881918.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16156..16383
                     /gene="yodD"
                     /locus_tag="ACHW4Z_002299"
     CDS             16156..16383
                     /gene="yodD"
                     /locus_tag="ACHW4Z_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR8881919.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16681..17496
                     /locus_tag="ACHW4Z_002300"
                     /pseudo
     CDS             16681..17496
                     /locus_tag="ACHW4Z_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17493..19187)
                     /gene="dgcQ"
                     /locus_tag="ACHW4Z_002301"
                     /pseudo
     CDS             complement(17493..19187)
                     /gene="dgcQ"
                     /locus_tag="ACHW4Z_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(19358..19540)
                     /locus_tag="ACHW4Z_002302"
     CDS             complement(19358..19540)
                     /locus_tag="ACHW4Z_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR8881920.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19619..20536)
                     /locus_tag="ACHW4Z_002303"
     CDS             complement(19619..20536)
                     /locus_tag="ACHW4Z_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR8881921.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20709..21629
                     /gene="yedA"
                     /locus_tag="ACHW4Z_002304"
     CDS             20709..21629
                     /gene="yedA"
                     /locus_tag="ACHW4Z_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR8881922.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21618..22088)
                     /gene="vsr"
                     /locus_tag="ACHW4Z_002305"
     CDS             complement(21618..22088)
                     /gene="vsr"
                     /locus_tag="ACHW4Z_002305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR8881923.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22069..23487)
                     /gene="dcm"
                     /locus_tag="ACHW4Z_002306"
     CDS             complement(22069..23487)
                     /gene="dcm"
                     /locus_tag="ACHW4Z_002306"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR8881924.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23554..24055)
                     /locus_tag="ACHW4Z_002307"
                     /pseudo
     CDS             complement(23554..24055)
                     /locus_tag="ACHW4Z_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(24134..>24369)
                     /gene="drpB"
                     /locus_tag="ACHW4Z_002308"
     CDS             complement(24134..>24369)
                     /gene="drpB"
                     /locus_tag="ACHW4Z_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR8881925.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6109 a         6272 c         6054 g         5934 t
ORIGIN      
        1 ctaccaaatt tatattcgtt cacaaaatag tcatgcattg tgtgcaatag aaacagttca
       61 gataaaaata gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat
      121 aatctcattg attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca
      181 aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt
      241 actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta acaagctgga
      301 tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag atgatggcga
      361 cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc aactgactgg
      421 tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa
      481 agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta
      541 tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc tgccagaatt
      601 cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca caggtgttgc
      661 aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta
      721 tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt tcggtttctc
      781 cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac
      841 taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt
      901 atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga
      961 aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga
     1021 agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc
     1081 taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata ttgatgtagg
     1141 tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat
     1201 tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg
     1261 attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc tttgcctccc
     1321 caacatataa gtggcgctct caagccactt tccttagaag cactatcttg cttctgacta
     1381 tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata atactcagtg
     1441 aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac ctgatatcta
     1501 atgtcataag catcccttta attatcccct caacctcttg taaatttccc actcttgagc
     1561 tccacatagc ccaaacgccg gatgcaaaaa aaatcatacg tgcatctctg aaattgtgat
     1621 ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca cagaagtcac
     1681 cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa ataaaaatcg
     1741 atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta gctctcttgg
     1801 tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact tgtaaccatt
     1861 ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac aacgcattat
     1921 ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc agacggcgat
     1981 gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc
     2041 taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata
     2101 aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt
     2161 cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata
     2221 ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcagttatcg
     2281 cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt tctgcaagat
     2341 aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga aataatatta
     2401 tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt tttccctgac
     2461 ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga tgtttatgca
     2521 tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc ttaccctatt
     2581 ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta agcatataac
     2641 caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt agagcccata
     2701 ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca ccaaatcctg
     2761 cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta ccatcacgtc
     2821 ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca gtccagtgcc
     2881 accatccact gcctgtagga gcatctggat catacacagt tacggcaaag cttttggtgc
     2941 cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca cacccaaatc
     3001 cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta ctagtgacct
     3061 gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa acgataagtt
     3121 ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg ccttgatctc
     3181 attatgcata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag gtgccccgaa
     3241 cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt attttttaat
     3301 gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc aacatcatgc
     3361 ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc aaactaatca
     3421 gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata taagccagcg
     3481 tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca gagggccata
     3541 accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct gcaagtattg
     3601 cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata ctggagagaa
     3661 ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt atccaagggg
     3721 ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac cccaggctaa
     3781 cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc gcgtttacgg
     3841 atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga aattgagtga
     3901 gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag catagtcaac
     3961 aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca ccagcttatt
     4021 acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg tcatcacatc
     4081 gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc gggcgactgt
     4141 ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac taatggtcgg
     4201 ttgcggcagt gattcctgcg cacgcgcact catcgccgta gcctgtaact ggctgataac
     4261 cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg gtaacaaagc
     4321 ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg gtttattggc
     4381 ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtg tgctgacccg
     4441 agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac gaagtgcgcg
     4501 atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct gcgtgcgaat
     4561 ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt cgcactgatc
     4621 ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga tcaggatggt
     4681 ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag cgaagccagc
     4741 ggcgctattg aggttccggc agataaagtt cacgaactgc gcctggcgca acaaggtttg
     4801 cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg tatcagccag
     4861 ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgaa
     4921 actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc
     4981 gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca
     5041 ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt tgctggtctg
     5101 ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca gtccaatacc
     5161 agcgggcgcg atcttaatga cgctcagttg aaatatgcca gcgatgtcga aggccgtatt
     5221 cagcagcgta ttgaagcgat cctgtcacct attgttggta acggtaatat tcacgcctag
     5281 gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac
     5341 ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt
     5401 tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac
     5461 gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc agcagcaggc gagcaccacc
     5521 agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc
     5581 accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg
     5641 gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag
     5701 caaattgaag ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc
     5761 aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagcact gccgttttgg
     5821 caacagcaag tgtttatcga tcagttgctg gctgccggtc gctggttgct ggttgctggt
     5881 actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc
     5941 cgaggcggtg aagactgtgc agcaacaggc gcaggcccgc gaggaagtgg aagatgcggt
     6001 ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc aacgactggg
     6061 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
     6121 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
     6181 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
     6241 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
     6301 agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt gctgcactga
     6361 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg
     6421 ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt attgaaacgc
     6481 tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat ccgcaaatta
     6541 tcgccaccat tctggtgcat ctgaggcgcg cccaggccgc cgatattctg gcgttgttcg
     6601 atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc gtgcagccag
     6661 ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
     6721 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg aaaacgcagc
     6781 aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca
     6841 tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc attcagcgtc
     6901 tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac
     6961 tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc
     7021 tcgccaagcg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc
     7081 tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc gaggatacct
     7141 atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc accaccggca
     7201 gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga acccagcctt
     7261 gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca ggcggggatt
     7321 gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc ccaggggctg
     7381 gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa
     7441 ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg
     7501 atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac ggtggataac
     7561 tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa
     7621 ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct cggcgcaact
     7681 ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg cggatgtaaa
     7741 gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca agaactctgc
     7801 cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc
     7861 tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
     7921 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
     7981 gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
     8041 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
     8101 cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag cagttgccgc
     8161 tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc gatggcctgc
     8221 ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
     8281 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
     8341 tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc
     8401 tggggatgat ggcacgctac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
     8461 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga cgtgcacgct
     8521 cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct
     8581 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
     8641 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
     8701 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
     8761 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
     8821 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
     8881 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
     8941 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
     9001 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
     9061 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
     9121 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
     9181 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaagaa cagctcaaaa
     9241 tgttgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga
     9301 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
     9361 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
     9421 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
     9481 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
     9541 ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac
     9601 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctca cgttgttgag
     9661 cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtagcaac
     9721 agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca
     9781 agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac
     9841 atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg acaaaaatac
     9901 gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct
     9961 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt
    10021 gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc
    10081 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
    10141 ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct
    10201 aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc
    10261 gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca
    10321 ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc
    10381 cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg
    10441 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
    10501 gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
    10561 gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggaag acgacgatac
    10621 gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt
    10681 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
    10741 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
    10801 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
    10861 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
    10921 gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg
    10981 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat
    11041 gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta
    11101 ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
    11161 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
    11221 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
    11281 tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc
    11341 cagtattagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
    11401 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
    11461 ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca
    11521 gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct
    11581 gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt
    11641 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
    11701 ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga
    11761 cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga
    11821 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
    11881 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
    11941 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
    12001 cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga
    12061 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc tgccgataga
    12121 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg
    12181 tactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
    12241 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
    12301 gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc
    12361 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
    12421 atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg
    12481 accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg
    12541 ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga agtggtggtt
    12601 gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc
    12661 cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg
    12721 ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
    12781 ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga
    12841 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag
    12901 atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc
    12961 cttcagcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc
    13021 ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg
    13081 gctgattacg cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg
    13141 cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt
    13201 cattccagcg attttactga tgatgaccag tttcacccgc atcattattg tttttggttt
    13261 attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc
    13321 actgtttttg acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta
    13381 ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc
    13441 gctgcgtgag tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact
    13501 ggcgaatacc ggcccgttgc aggggcctga agctgtgccg atgcgcattt tgctcccggc
    13561 ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt
    13621 tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc
    13681 ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca
    13741 attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga
    13801 atcggtcatg atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct
    13861 gttgctggta gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat
    13921 taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat
    13981 tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct
    14041 gccgtatatc atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc
    14101 tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca
    14161 attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg
    14221 ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg
    14281 tggctggccg tacagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc
    14341 tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg
    14401 acatttgtcg atcctggcag ccatctcaat atgcccgttt tagcgcgcat catggatatg
    14461 ctggcattac tgctgttcct gacatttaac ggtcatttat ggttgatttc actgctggtc
    14521 gatacctttc acaccctgcc gattggtagc gaaccgttga acagcaatgc gtttctggca
    14581 cccaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt accgctcatt
    14641 actctgttgc tgaccctgaa tctggcatta ggtttactga atcgtatggc cccgcaatta
    14701 tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt aatggcggca
    14761 ttaatgccgt taattgcgcc tttttgcgaa catttattca gtgaaatttt taatttgctg
    14821 gctgatatta ttagtgaatt accattaata taattccgta acgtttatca tgctacccta
    14881 aggattatcc gaaaaataat atctgcgaac atcttccagg atactcctgt agcgaaatat
    14941 ttgttttaag ctcactcaca tatcgcaaca tttactttaa gacaattcca ggcaaattat
    15001 acaacacttt acgggatagt aagtccgcct gaaaaattgc gagagtggcg cattaggtga
    15061 cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta tcgttacgca
    15121 ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac acccgactag
    15181 gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac gacattgaaa
    15241 ccgttgatga ccttgccata gcttgtgatt cacagcgtcc ttcagtggtg tttattaatg
    15301 aggactgttt catccacgat gcttctaaca gtcagcgcat caagcacatc attaatcaac
    15361 atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttt gatgaatatc
    15421 tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg
    15481 atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta aatatgccga
    15541 cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca ggtcagggaa
    15601 ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg cataaaggta
    15661 atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc gtccgactga
    15721 cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg actggtggtt
    15781 tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac cgtcctgatg
    15841 ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa ccaggtacat
    15901 tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg gaccgccatc
    15961 cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc tttgtgccag
    16021 tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga ttttctcgac
    16081 ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac agagtaacgg
    16141 ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa acgtgaggtc
    16201 agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag cgaagttcat
    16261 cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca tacatggcgt
    16321 gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga agtgaatcgt
    16381 tgaatgcaca ataaaaaaat cccgaccctg agggtgtcgg gatgaaactt gcttaagcaa
    16441 gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc ttatctgcaa
    16501 tttttttcgc tgacaagaaa atattcgcgc tatgaatgat taacgctact tatgaataga
    16561 aatgtgacgc gcgtcacccc ttggtggctc tggtgtcaac gggatcgctc ccgaaaaaat
    16621 catctttgat tatttgtgct gttttgagaa tcatcctggt aaggggagta ctgcgcgacc
    16681 atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac cctgctggac
    16741 agtcatagtt atgactggca accggcagcc ccctggctca gccgtttaca cgaagccaat
    16801 attcccgtca ttctctgtag cagtaaaact ttagcggaaa tgctgtactt gcaaaaaatg
    16861 ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca gcttgctgaa
    16921 caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca tggcgaaatc
    16981 tgccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac ttttgatgat
    17041 gtcgacgatg caaccattgc cgaatggacg ggattaagcc gtagctaggc ggcgctgacg
    17101 cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa
    17161 tttatcgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg cttctggcac
    17221 gtactggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc gacctatcaa
    17281 caattgtcag gcagacgccc aaccacactt ggcctgggcg atgggccaaa cgatgcgcct
    17341 ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga aggggtacat
    17401 ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg
    17461 cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc gaatacccga
    17521 ttgcgcccgg cctgttttgc gaggtaaagc cgacggtcgg ccaatgactg gagttgttca
    17581 aaatcataat caccggtttc ctcgctgcta cttaccccca gcgaggcact gatgcgtatc
    17641 gttgtactct tggcgatcag catctctttt tcatttaact tcaggcgaat acgttctgcg
    17701 acctccgcag cctgcgtcag attcgcgcct ggcagaatca cacaaaactc ctcaccaccg
    17761 acccgcccgg caacgtcctg cgcacgcaag gaactgctaa ttaatccggc agcatgagaa
    17821 agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa atggtcaaga
    17881 tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttagc gagcggacgg
    17941 gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg ccacgcctgc
    18001 cactgcaacg agctttgcag aacatacatg ctgctgacca tccggcgaat cacataccag
    18061 gagaggagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat gctgatactg
    18121 ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc gcgccagcac accgtcgaaa
    18181 tggtccagac gttcccagct aacatagcga ctgtccatac gaatgccgcc ccgcgtgtca
    18241 tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt ccctgttgga
    18301 tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg atactcacca
    18361 tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tagtacgcac gggaatactc
    18421 atccccagca cgccatacca gtagttatta ctgtctaccg gaacactgac ggtaacctgc
    18481 ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc gcggtgacga
    18541 ttttctcgtt gcgaatggcc gataaaccag ggttgggtga catagccgta ataacgcgtt
    18601 ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccagc acgcgagaca
    18661 tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca gtcgcagcaa gtaaccaact
    18721 tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag gaaattcccc
    18781 tcgctgacta atgcatccga tacgccattg actggcaggg tgcgccgtcg gttgagttcg
    18841 atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt acgcagagag
    18901 gtgaaatcca gtggcgccac gagagcttcg cgcatgccat tacgcaaaaa gatcagtttg
    18961 tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag atgattacgc
    19021 tagctggaga tataggcatc ttccagcacc accacttcac gccaggtgag caaggtggaa
    19081 aaaagcaata ccacgataaa gcagagatta acgacatgac cgggccccag gcggcgtgcg
    19141 agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct gacgactccc
    19201 aacctgcttt tttatgattc tggcataacc cggcaatagc gtaccgcaaa acagcgataa
    19261 ttttataatg gtggcatgat aagacgcgac tatcattcat tgcaggcatt gggacaccag
    19321 gccggatgtg atccggcctg gtgttaatga taccctttca aacttcttcc gcagaacgac
    19381 tcccctcccc cggaacaaga tcggtttcat gaaaagcttc ccgcttgacc ccgtaaccgt
    19441 cataccaacg acactcaacc ataccgctgg agtatccagt gacaatcatc cgcgggccgc
    19501 cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc ttttttcgag
    19561 tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagccag tgcgagtatt
    19621 aatgtgcctg accgcgcatt ttcgctactg ctttcactcc cagcaccacg atgccgccga
    19681 tgataaatcc aagaatcaga tttaaaacag tcggtaatat catcgccacc actgcacttt
    19741 gctgcccggc gatatgttca atagcgtgat gcagcggcgc aataccatgt accacaatcc
    19801 cgccgccgac gaggaacatc gccagcgtgc cgacaatcga taatgctttc atcagccaag
    19861 gcgcgataat caataaccct ttacctaatg cctgcatcag cgcgctagat ttttccgcca
    19921 gccaataccc taggtcatca atcttaacga taacccctac cagaccgtag acgcccacgg
    19981 tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc gcctctgcca
    20041 caatccccag cgtgatggcg acgatttccg cagacaaaat aaaatcggta cgaatcgccc
    20101 ctttaatttt gtccttttca aacttcagcg gatcctgcgc cgccagcttc tccagacgct
    20161 gctggctctg cgctggatct tctttatgtt tacgcgcctc cagcatatgc agcactttct
    20221 ctaccccttc aaagcagaga aacgcgccac caatcatcaa cagtggcgta atcgcccacg
    20281 ggataaacgc actgatgatc agcgccagcg gcaccagaat cactttattg atcagcgatc
    20341 ctttcgccac gccccagacc acgggaagtt cccggttggc ccgcacgcct gaaacttgtt
    20401 gcgcattgag cgataaatca tcccctaata caccggcggt tttcttcgcc gccagtttgc
    20461 ccatcacaga gatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc agtaaactac
    20521 tcccggccaa aagcattccc tctcattttt attgttgaag ggtgtaagta tgaagcaaaa
    20581 acggcagctc tgatacttgc ttcacaggtc aacatttatt taacaatcaa gtgcaattat
    20641 atctctcgcc agcagaatga ttttcacgtt tactatgaac caccttttta tttccgccgt
    20701 gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt gcgttgtata
    20761 tcatttgggg ctcaacctat tttgtcattc ggattggcgt gggaagctgg cctccgttaa
    20821 tgatggcggg cgttcgattc caggcagccg gtattttatt gctggcattt ttgctactgc
    20881 gcggacacaa acttcccccg ctacgtccgc tgctcaatgc cgcgctgatt ggcctgttat
    20941 tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt ccttccggca
    21001 tcgccgccgt agtgattgca accgtgcccc tctttaccct gtgcttcagt cgcctgtttg
    21061 gcattaaaac gcgcaaactg gaatgggtgg gtattgccat tgggcttgcc ggaatcatca
    21121 tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg attttaatcg
    21181 gctcgattag ctgggcgttt ggctcagttt atggctcgcg cattacctta cctgtaggga
    21241 tgatggcggg tgcgattgag atgctggcgg caggcgtggt gttaatgatc gcatcgatga
    21301 ttgcgggtga aaaactgacg gcgctccctt ccctgtcagg cttccttgcg gtcggctatc
    21361 tggcgctgtt tggttcgatt atcgccatta acgcttatat gtatttaatc cgtaatgtca
    21421 gtccggctct cgccaccagc tacgcctacg ttaacccggt ggtcgcggtc ttgctgggta
    21481 cgggactggg tggcgaaacg ctgtcgaaga ttgaatggct ggcgctcggc gtaattgttt
    21541 tcgcggtggt actggtcacg ttgggaaaat atctcttccc ggccaaaccc gttgtcgcgc
    21601 cagttattca ggatgcatca agcgagtaaa tgaatcccct gcgtgtcgat ctgcgcgctg
    21661 gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgccgcatc cgtcagcttc
    21721 tcgcgcctac gtaacgcaca ctcccagaca atcagtacgc gccagccgag ctcctgcaag
    21781 cgactgatat cgcggcgatc gcgttcaaca tttttaccta tcttctccag ccagaactca
    21841 gttcgagtcg caggcacttt aaacagatag cagtgatgat gatgccagaa gcagccgtgg
    21901 gtaaatatca cgcagcgata ttcatcaacg acaaaatccg ggcgtccggg cagactggcg
    21961 tcctgaacgc gaaacgcaag gccctgcccg gttaacaaac tggcgaggcg cttctcaatc
    22021 gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gggtggcctt atcgtgaacg
    22081 tcgaccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt ttggctcaag
    22141 cagttttgcc accgcggcaa agaccggcac gaccaccgag ttgccgaact ggcgataggc
    22201 ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc ggcgcttcaa aacccattaa
    22261 gcgcgcgcat tcacgaggcg ttaaccgacg tggacgatgt tgctgattca gcggatcgtc
    22321 aaagtctttc tcacccgtgg ccatatccca accgcgatcg attaaaattt ccgcgccatc
    22381 tttgtaataa cgagcagaca gcgtacgggt gacgctttgc ggattgttcg gataaaccat
    22441 tccataaccg aagccgttac cgcgcgcctg atgttttttc gcatatcgat agaggtactt
    22501 ccacagcacc ggcgtcagga tatatttcgc ctcaaccatc gggtccagca gctgcgccag
    22561 cgtcactcgc tgtgcaggga aacattcgct gatatcacgc agagtaaaat cggctttcag
    22621 attcagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg gcaggaaatg
    22681 tttaccatcg atgattttcg gatcgtccgg cccgttatct tctgcatcgg caacgtcata
    22741 gcccagttcg tccagcgtct gcatgatgat gcggaacgtt tttcccttat cgtgactctt
    22801 caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga taatgcgcac
    22861 cacatcaaag aacagcgtgc cctgggtatc gcaggcaaaa ccgtgcgccc gcccgagcga
    22921 gttctttttc gatacgccag ccagcgaaaa tggctgacaa gggaaaccgg ccagtaaaac
    22981 atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc gcctcatcac tcacgccttc
    23041 ttgatgactg agggtgatgt cgcggatatc ttcattaaaa tgatgcgtcg ccgggtcgca
    23101 ataatggttg gctttataag tgcgtaccgc atgtttgttc cattcgctgg taaacacgca
    23161 ctgtccacca atcgattcaa aaccacgacg gatgccgcca attccggcaa atagatcgat
    23221 aaagcgaaac gcataatgcg gatgatgttc cggtggtttg ggtaacaacg tttgcagatg
    23281 ggcaaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg cacgttttaa
    23341 aattgccgcg ctccagtgat tctcacccac accattaagc tgcgccacca gcattttaac
    23401 atcataaatt tgcagcagtt tctccagcat tgcctgtgcg gcattcccgg tgctgtatga
    23461 atcggttact gatatatttt cctgcataga tttaaccggc catctgagat ggccgacagg
    23521 ttaacataat tactgccgtt tagggccacg cgctcagcca gcgggtgaaa acgcatctat
    23581 caccttgtga tcgatacctt cattctcccc cgccagttcg gcaccgagct tcgccatcaa
    23641 acagcgccaa gctcaatgcc cctgaaacgg caaatacgcg cgccaggcca atcgccccca
    23701 gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag ggggcgattt
    23761 gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcaattttc tccgccggaa
    23821 attgcacgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg attgaagaac
    23881 gctgccgttg cggatgattt ttcgccaggc tcacgatatc gtgaaaataa caggcggtta
    23941 aaatcaccag catatcgaca tcgtcatctg ccgccagttt ctgtgccgta gcccagacac
    24001 ggcgaaaatg acacaggtca tgcgccgcgt cctgatgttg atgatggttt ttcaaccagt
    24061 tctcgaacta tgcctgccag tgttgtaact ccatacgcgc tccgttgcca aagagtctca
    24121 accttagcag tttttattca tagcgtctgc aacgtgcggg acgaggtcgg gtgtaccatg
    24181 caatgccgcc taacaaggca ccaaccaggg ccataaacag aaatccgacc aatgctccta
    24241 accacttccc cgcggtagat cctaaatcac tttcaaatcc cgacacgggg gcatcgggga
    24301 tccggctcat cacccagatg tatcgcgcca ttgcccagac gaaatagcca caccatgtat
    24361 agaaagccc
//
LOCUS       DAZSNG010000054        24184 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZSNG010000054 DAZSNG010000000
VERSION     DAZSNG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.055"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(25..597)
                     /locus_tag="ACHW4Z_002309"
     CDS             complement(25..597)
                     /locus_tag="ACHW4Z_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR8881926.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACHW4Z_002310"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACHW4Z_002310"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR8881927.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHW4Z_002311"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHW4Z_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR8881928.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHW4Z_002312"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHW4Z_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR8881929.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACHW4Z_002313"
     CDS             complement(3559..5340)
                     /locus_tag="ACHW4Z_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8881930.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACHW4Z_002314"
     CDS             complement(5333..7105)
                     /locus_tag="ACHW4Z_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8881931.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHW4Z_002315"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHW4Z_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR8881932.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHW4Z_002316"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHW4Z_002316"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR8881933.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="ACHW4Z_002317"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="ACHW4Z_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="ACHW4Z_002318"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="ACHW4Z_002318"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR8881934.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHW4Z_002319"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHW4Z_002319"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR8881935.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="ACHW4Z_002320"
     CDS             complement(11661..12032)
                     /locus_tag="ACHW4Z_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR8881936.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHW4Z_002321"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHW4Z_002321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8881937.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHW4Z_002322"
     CDS             complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHW4Z_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR8881938.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHW4Z_002323"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHW4Z_002323"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR8881939.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHW4Z_002324"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHW4Z_002324"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR8881940.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHW4Z_002325"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHW4Z_002325"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR8881941.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHW4Z_002326"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHW4Z_002326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR8881942.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHW4Z_002327"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHW4Z_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR8881943.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="ACHW4Z_002328"
     CDS             21803..22381
                     /locus_tag="ACHW4Z_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8881944.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="ACHW4Z_002329"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="ACHW4Z_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR8881945.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6666 c         5828 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gtta
//
LOCUS       DAZSNG010000055        24086 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZSNG010000055 DAZSNG010000000
VERSION     DAZSNG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24086
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.056"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHW4Z_002330"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHW4Z_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR8881946.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHW4Z_002331"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHW4Z_002331"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR8881947.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHW4Z_002332"
     CDS             1531..2478
                     /locus_tag="ACHW4Z_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR8881948.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHW4Z_002333"
     CDS             2548..3354
                     /locus_tag="ACHW4Z_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8881949.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHW4Z_002334"
     CDS             3364..3771
                     /locus_tag="ACHW4Z_002334"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR8881950.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4262
                     /locus_tag="ACHW4Z_002335"
     CDS             3771..4262
                     /locus_tag="ACHW4Z_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR8881951.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQL
                     IAN"
     gene            4328..5125
                     /locus_tag="ACHW4Z_002336"
     CDS             4328..5125
                     /locus_tag="ACHW4Z_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR8881952.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5136..5924
                     /locus_tag="ACHW4Z_002337"
     CDS             5136..5924
                     /locus_tag="ACHW4Z_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR8881953.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5976..7205
                     /gene="sorE"
                     /locus_tag="ACHW4Z_002338"
     CDS             5976..7205
                     /gene="sorE"
                     /locus_tag="ACHW4Z_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR8881954.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7587..8276
                     /gene="pepE"
                     /locus_tag="ACHW4Z_002339"
     CDS             7587..8276
                     /gene="pepE"
                     /locus_tag="ACHW4Z_002339"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR8881955.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8367..9998)
                     /locus_tag="ACHW4Z_002340"
     CDS             complement(8367..9998)
                     /locus_tag="ACHW4Z_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR8881956.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10218..13901)
                     /gene="metH"
                     /locus_tag="ACHW4Z_002341"
     CDS             complement(10218..13901)
                     /gene="metH"
                     /locus_tag="ACHW4Z_002341"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR8881957.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14101..14925
                     /gene="iclR"
                     /locus_tag="ACHW4Z_002342"
     CDS             14101..14925
                     /gene="iclR"
                     /locus_tag="ACHW4Z_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR8881958.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15242..17426
                     /gene="espL4"
                     /locus_tag="ACHW4Z_002343"
                     /pseudo
     CDS             15242..17426
                     /gene="espL4"
                     /locus_tag="ACHW4Z_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17395..19132)
                     /gene="aceK"
                     /locus_tag="ACHW4Z_002344"
                     /pseudo
     CDS             complement(17395..19132)
                     /gene="aceK"
                     /locus_tag="ACHW4Z_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19316..20620)
                     /gene="aceA"
                     /locus_tag="ACHW4Z_002345"
     CDS             complement(19316..20620)
                     /gene="aceA"
                     /locus_tag="ACHW4Z_002345"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR8881959.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20650..22251)
                     /gene="aceB"
                     /locus_tag="ACHW4Z_002346"
                     /pseudo
     CDS             complement(20650..22251)
                     /gene="aceB"
                     /locus_tag="ACHW4Z_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22520..23449)
                     /gene="metA"
                     /locus_tag="ACHW4Z_002347"
     CDS             complement(22520..23449)
                     /gene="metA"
                     /locus_tag="ACHW4Z_002347"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR8881960.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23606..24049
                     /locus_tag="ACHW4Z_002348"
     CDS             23606..24049
                     /locus_tag="ACHW4Z_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR8881961.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6145 c         6107 g         5890 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatattaa cgcatttcat gagttaaata atcttggtgt catactggat ttacgcgtcg
     4201 tagcgtcaga tccctcaatc aatattatcg acaaaataaa cgaacagtta atcgcaaatt
     4261 aaataatagc gcctgtgtac atatgcttta acaggcaggg atgacgacac ttaaaaggtg
     4321 acaaatcatg gaaataagta ccctacaaat cattgctata tttctttttt cctgtattgc
     4381 cggaatgggc agcgtgctgg atgaatttca gactcatcgc ccgttaattg cctgtacggt
     4441 gattggttta attctcggtg atttaaaaac cggaataata ctcggcggta cgctggaatt
     4501 gatagctctc tgctggatga acgtcggcgc ggcgcaatct ccggattctg cactcgccag
     4561 cataatctcc gccattctgg ttatcgttgg tcagcaaagc atcgccaccg gaatcgccat
     4621 cgcgttgcct gtggctgcgg caggccaggt gctgacggtg tttgcccgta ccatcaccgt
     4681 agtgttccag cacgcggcgg ataaagcagc agaagaggcg cggtttcgca ctctcgatat
     4741 tctgcatgtc tccgcgcttg gcgtgcaggc gctgcgcgtc gccattccgg cactgattgt
     4801 ctcactgttc gtcagcgccg atatggtcag caatatgtta aacgccattc ccgaatttgt
     4861 gacccgagga ctacagattg ctggcggttt tatcgtggtg gtcggttacg ccatggtgct
     4921 acgtatgatg ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc tcgcaggtgg
     4981 ctacctcgat ctcagcctgc tggcgttcgg tggcgtcggc gtgatcatgg ccctggtcta
     5041 catccagcta aatccacagt ggtgtaaagc tgaacctcaa ctccaggcca ccgcctccac
     5101 cgcccttgac caacttgacg attaacggag ccatcatgga acagagaaaa attacacgca
     5161 gcgatctggt gagcatgttt ctgcgctcca acctgcaaca ggcgtccttt aactttgaac
     5221 gtattcacgg gctgggcttt tgctacgaca tgatccccgc catcaagcga ctttacccat
     5281 taaaagagga tcaggttgcg gcgctcaggc tacacctggt gttcttcaat accacgccag
     5341 ccgtatgtgg cccggtcatc ggcgcggaaa ttgatgacgg taccatcaac ggcatcaaag
     5401 tcggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga acgctgcgcc
     5461 cgattaccgc cgcgctcggc gcatctctgg cactttcggg caacattctc ggcccgctgc
     5521 tgttcttctt tattttcaac gcggtgagtc tggcgatgaa gtggtatggc ctacagctcg
     5581 gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg caaaaactca
     5641 ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt gctggtgacc aaatggacgt
     5701 caatcaacgt accgttggtg gtttcacaaa cgcatgccgc cgatggctcc accgtcacca
     5761 tgaccgtgca gaacattctc gaccaacttt gccctggttt gctggcgctc ggtctgacgc
     5821 tactaatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt ttcgccctgt
     5881 ttggcttagg gattatcggc aatgctctgg gcttcctgtc ctgattcttc gccccggcac
     5941 gactgccgag gccatcgctc aacatgaggt ggtttatgaa aacaacagct ctgcgtcttt
     6001 atggtaaacg tgatttacgc ctggaaacct ttgaccttcc tgaaatgcag gaggatgaaa
     6061 tcctcgcaac ggtggtcact gacagcctgt gcctctcttc ctggaaagag gccaatctgg
     6121 gtgaaaacca taaaaaagta cccgacgatg tgacgaccaa cccaatcatc atcggccacg
     6181 aattttgcgg cgatattctg gccgtgggta aaaagtggca gcacaaattc cagccgggtc
     6241 agcgttatgt gattcaggcc aacctgcaac tccccgaccg cccggattgc cccggctact
     6301 ccttcccgtg ggtagacggc gaagcgacgc atgtggttat tcccaacgag gtcatggaac
     6361 aatattgcct gctggcatac gacggcgaaa cctattttga aggctcactg gttgaaccgc
     6421 tttcctgcgt gattggcgcg ttcaacgcca actatcatct tcaggaaggt agttataacc
     6481 acacgatggg gattcgcccg caagggcaca cgctgatcct cggcggcacc ggaccaatgg
     6541 gactgttggc gattgattat gcgctgcatg gaccagttaa cccgtcgctg ctcgtcatta
     6601 ccgataccga caacgacaaa ttgagttatg cgcgcaagca ctatccatca gaaccgcaaa
     6661 cactgattca ttatctcaat gccaccgatg cagcatttga tacgttaatg gcgctgagtg
     6721 gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa tgaaggactg gtgactctcg
     6781 cctcttcctt gctggcgaca gatggttgcc tgaatttctt cgccggaccg caggataaac
     6841 atttcagcgc gccaattaat ttctacgatg tgcattatgc atttacccac tacgtgggca
     6901 cgtcaggcgg caataccgac gacatgcgcg cggcggtcaa actgatcgaa gagaaaaaag
     6961 tgcaggccgc aaaagtggta acacatattc ttgggctgaa tgccgcgggc gaaaccacgc
     7021 ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa tacctgccgc
     7081 tgacgtcact cacgcagatt caggatcaag cactggcggc gattctggcg cgtcatcagg
     7141 ggatctggtc cggtgaggtg gagcaatacc tgctcgccca tgcagaggca atttcccatg
     7201 attaatcgcg ctacttaact ctgcatgtcg ctggctggtc gccccagcaa cttcgcgacc
     7261 ggatgtgcgg ctgattgctg aagcagcaca atttgcacga gaaggataaa aaatatcagc
     7321 gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg atgcggaatg
     7381 aagccttatt cggtctacat aattttgaaa atccaatata ttgcagttat ctggtaggcc
     7441 aggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa
     7501 tacgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag catgccttcc
     7561 gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac tcgacgctgc
     7621 cgggtaaagc ctggctggaa catgcactgc cgctaattgc tgaacagttg cagggtcgcc
     7681 gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgggatgat tacacagaga
     7741 aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat agcgttgtcg
     7801 atcccgttgc cgcgattgaa aatgcggaaa ttgtgattgt cggcggcggt aatactttcc
     7861 agttgctgaa acagtgccgc gagcgcgggc tgctggctcc aattactgac gtggttaaac
     7921 gtggcgcgct gtatattggc tggagcgcag gcgctaacct tgcttgccca accattcgta
     7981 ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat ctgttcccgt
     8041 tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt gaaacccgtg
     8101 agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt ggtctgccgg
     8161 aaggtaactg gatcacagtg agtaaaggtc acgctacgct gggtggcccg aacaccactt
     8221 atgtgtttaa ggctggtgaa gaagcggttc cgctggaagc tggtcaccgt ttttaagtga
     8281 attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc ttgacgcgtc
     8341 ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccttcatc ttcatccggc
     8401 tgttccagca cactgtaagc caccgaacaa aacagcgagt tcaggcgctg catatctccc
     8461 agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg atgcaggcga
     8521 tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa aacgatgttt gctgcgacgc
     8581 aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag ctttaaatta
     8641 ctgagcaatt gctcatagag cgcatccagt tccttcaacc catcaagcga aaatgcccgc
     8701 cgtgctgcca gcgatttatc ggcaatttcg ctgcccatgc gctcgacgat atcggaggcc
     8761 tgttcaaggt tgagcgacat ttcgatgatc tccgcccagc ggcgcgactc ttcctccgcc
     8821 aactcttctt ttggcatccg cgccagatac agcttaatgg cggtgtagag aacgttgata
     8881 tcatctgcca gcttacgcag ctctttctcc tgccgtggct cgccgtgcat cactttattc
     8941 agcccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc
     9001 gccagagcaa gcgtgggcgt gtccagcgcg ctgacatcca gatgtttagg ccgtagctgg
     9061 gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaaca
     9121 aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata gatcaccagt
     9181 tccgcttttg gcaatgacag cttccccatt gtctctgcca gcaaatggac aaacggcagg
     9241 ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg gcgggctgcg
     9301 gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt agctccaatc
     9361 accagacaga gcgccacggg gaaggagata atgcctgcag cggtcagagt cgcggtcagc
     9421 agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat cagcgcatcc
     9481 agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc
     9541 ggcgttacgg cctgcacaat caactccagc gccagcaaaa tcagcccaag accaataccg
     9601 acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata
     9661 aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc cattagcgcc
     9721 gtcccgacat ctgcccccag cacaatgacc agagccggtg cgagggctac cagatcctga
     9781 gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag tgcggtaacg
     9841 ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc
     9901 aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc ccagaccagc
     9961 agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcagattc tccttatcgt
    10021 tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac tggcatcttt
    10081 actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgtt tccagtataa
    10141 gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa
    10201 agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag ccagcgctca
    10261 acatcggaga cgctcatacc tttacggcgg gcatggtctt caacctgatc gcgctgaatt
    10321 tgcgccaccg cgtagtactt gctgtccgga tgactgaaat accaacccga aaccgatgca
    10381 ccaggccaca tcgcgaaaga ttctgtgagt ttcatgccag tatgtttttc cacttccagc
    10441 agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccggatagcc cggtgccgga
    10501 cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc gttcggcgca
    10561 tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc ttcggctaaa
    10621 cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca
    10681 tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat gtaatctgct
    10741 ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa tcccgttttt
    10801 tcggtctgtt gacgcagatg gtggctgacg ttgatcacat gggtgcgcgt ttcgtcacgg
    10861 tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac gccacgcgga
    10921 ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tggcgtcttt aaacagccgc
    10981 tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgag gatacttccc ggccagcgac
    11041 caggtcataa agaacggtgt ccagtcgatg taattacgca gcgtttcgat gctggcttcc
    11101 acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca gtcaaatgcg
    11161 aaatcgttat cgcgcgccgc ttccagcgtg accggtggtg tgcgtggttt cttgcgcccg
    11221 tgctgaatac gcacggtttc gtactccttg caggtacgag cgacaaaatc atcacgctga
    11281 gtatcggaaa gcagcgccgc taccacacca acggtgcgcg aggcattctg cacatacacc
    11341 gtagggccgc tgtagttctg ctcgattttc accgccgtgt gcgcttttga ggtggtcgcg
    11401 ccgccaatca gtaacggaat ggcgaagccc tgacgctcca tctctttcgc cacgttaacc
    11461 atctcgtcca gcgacggcgt gataagcccc gaaaggccaa tcaaatcagc attcacttct
    11521 ttggcgatac ggagaatttt ttccgcaggc accataacgc cgagatcgac aatttcgtag
    11581 ttgttacatt gcagcaccac accaacgata tttttaccga tgtcgtggac atcgcctttc
    11641 acggtggcga tcaccatctt gccgttggtt ttaccctgct ctttgctggc ttcaataaac
    11701 ggttcgagat acgccactgc ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg
    11761 aacattttgc cttcgccaaa cagatcgccg accacgttca tgccgtccat caacgggcct
    11821 tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcagtatc ctgctcgata
    11881 aactcggtta tgcctttgac cagcgagtat tccagacgtt tattcacgtc ccacgagcgc
    11941 cactccgctt gctgggcgtt agcagtgtcg tccgctttac tgccgcgata tttctcggca
    12001 agctccagta aacgctcagt gccatcgtca cgacgattaa gaatcacatc ttccaccgcg
    12061 tcgcgcagtt cagcgggtag gtcgtcgtaa atcgccagtt gcccggcgtt gacgatcccc
    12121 atatccatgc cattgcgaat agcgtagtag aggaacactg cgtgaatggc ttcgcgcacc
    12181 ggatcgttgc cacggaatga gaaagaaacg ttagatacgc cgccggaaat cagcgcgtgc
    12241 ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt cctgcgcgta gttgttgtgc
    12301 tcttcaatac cggtggcgac cgcgaagatg ttcgggtcga agatgatatc ttctggcggg
    12361 aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat tttccgtgcg
    12421 cgagtatcgg cctgtccctg ttcgtcaaag gccatcacca ccactgccgc accgtagcga
    12481 cgcaacaatt tcgcgtgatg gataaaggcg tcgacgccct ctttcatcga gatagagtta
    12541 acaatgcctt tgccctggat acacttcaga cctttttcaa tgacatccca ttttgaggag
    12601 tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt gagaaaacgc
    12661 accatcgccg cttcggcatc gagcatccct tcatccatgt tgatatcgat aatctgcgcg
    12721 ccgttttcca cctgctgacg cgccacatcc agcgcctcgc tgtatttctc ttctttgatc
    12781 aggcgcttga acttagcaga gccggtgacg ttggtgcgtt cacccacgtt cacaaacagg
    12841 ctatcgtcgc caatgttcag cggctccagg ccagacaaac ggcaggctac gggaatttcc
    12901 ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat atgttgcggc
    12961 gtggtcccgc agcaaccacc gacgatattg agaaagcccg cttgcgccca ttcacgtatc
    13021 tgttttgcca tcgtgtcggc gtcgagatcg tattcaccaa aggcgttagg cagcccggcg
    13081 ttcgggtggg cggtgacgta gcattccgca atccgtgata gttcctgcac gtactggcgc
    13141 agttcatcgg gccccagcgc acagttcagg ccaaaggtca gcgcttcggc gtggcgcaat
    13201 gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg
    13261 gtgccggaga tcataatcgg cagctcaacg cccagcgctt caaactccgt tttcaccgca
    13321 aagaccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcaggat cagatccgca
    13381 ccaccttcca ccagcgcttt ggtggattcg cgataggccg ccgccagctg gtcaaaagtg
    13441 atgttgcgga atgccggatc gttgacgtcc ggagaaatag acgccgtgcg gttggttggg
    13501 ccaagaacac cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca ctcgtcagca
    13561 caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga ttccatctgg
    13621 taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat atccgcgccc
    13681 gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttactgag taccagcagg
    13741 tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc gttcaccacg aaaatcggct
    13801 tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccaacac cagaatacgt
    13861 tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc cgacacgctt
    13921 aacccagatt taataaaaat tcaacaaacc atactggaat aaacgcatct gtggtaaaag
    13981 cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt tcagcaacta
    14041 ttgcattagc taacaataaa aatgaaaata atttccacga tacagaaaaa ggaggctgtc
    14101 atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg
    14161 actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc
    14221 aatggcagcg tggcactcac agagctggcg caacaagccg ggttacccaa ttccacgacc
    14281 caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggtcgg cgaactggga
    14341 cactgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca gagccgtaat
    14401 ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg cgaaacggtc
    14461 aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgacca ggtacagtgt
    14521 acccacctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca cgcttccggt
    14581 gcgggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct gctgcaccgc
    14641 aaagggttac atgcctatac ccacgcaact ctggtgtctc ctgtgcattt aaaagaagat
    14701 ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc gctggggcta
    14761 cgttgccttg cagcgtgtat tttcgatgag caccgcgaac cgtttgccgc aatttctatt
    14821 tccggaccga tttcacgtat taccgatgat cgcgtgaccg agtttggcgc gatggtgatt
    14881 aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt tctggcgggc
    14941 agaggcaata ttctgcccat catacctgag tggcaataga ataagggtgt ctgttaatcg
    15001 cattgacgcc aaaataactt aatgtcatac acttcacaca cttttattaa ctcagcatta
    15061 tttttaaaca tcaaaccact taattattaa caaaatcgca agatctcatt ccaccgaaac
    15121 ttttacggct gcattatgag attgagcgat ttatgatatt tttttagttg taatcatatt
    15181 aaattttttc actgttctga tagttaaaat tcaagacatc aataaacaat gagatattta
    15241 aatgattact cgtattcctc gtagttcttt ctctgcaaat attaataata cagcccagac
    15301 aaatgaacac caaaccctga gtgaattgtt ttataaagaa ctcgaggata aattttctgg
    15361 caaggagctg gcgacgcctc tattaaaaag cttctcagag aactgtagac aaaatgttcg
    15421 tcatatcttt agcaacaagg attttgtcat taaattttcc acgtccgtct tacaagctga
    15481 taagaaagaa attacgatga ttaataaaaa cgaaaacacg acactcactc aaaccattgc
    15541 tacaatattt gaagaatacc taatggaaat tttacctcaa cgctcagaca ctcttgataa
    15601 acaagaatta aaccaaaaat cagatagaaa agaaaaagaa ttcccaagaa ttaaacttaa
    15661 tggtcaatgt tatttcccgg ggcgccccaa aaccgtatag tataccgaca cattgctgca
    15721 caatatatta atgatattta tcagaatgtt gattacaaac cccatcaaga tgattactct
    15781 tcagctgaaa aatttctcac tcacttcaac aaaaaatgca aaaaccagac tttggcgttg
    15841 atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg gctagttatg
    15901 aaagcatatt ttgacaagat ggaatcaaat ggcatcagtg ttatggcagc catattactg
    15961 gtggataacc atgctttgac ggtccggcta agaataaaga acacaactga aggatgtacc
    16021 cattacgtgg tttcggttta tgatcctaat gtaactaacg ataaaataag aattatgagc
    16081 gaaagcaaag aggatattaa acactattct ctgatggatt ttatgaatgt agattatagc
    16141 ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt
    16201 ccgaaagaac agctattgat gttaaaagga actgtggatg aaataacccc tccattatca
    16261 ccagcaacga tgaatttgct aatggcaatt ggtcagaatc accaacttac gcaactgatg
    16321 attcagctcc agaaaatgcc agaactacat agaacagaaa tgttgactgc ctataatagt
    16381 attaacctcc ccggtttata tttggctata aattatggca atgcggatat cgttgagact
    16441 attttcaatt cattgtcaga aacaggatat gaagggttac tctcgaaaaa aaatctcatg
    16501 catattctgg aggcaaaaga taaaaatggt ttttctggat tatttttagc gatatcacgt
    16561 aaggataaaa atgttgtaac ctcgattctg aacgtcttac ctaaactggc cgcaacacat
    16621 catttagata atgaacaggt gtataaattc ctgagtgcca aaaatagaac gtccagccat
    16681 gttttatacc atgttatggc gaatggtgat gccgacatgc tgaaaatttt tttggtcgcg
    16741 ttacctttgt taattcgcac atgtcatttg actaaagaac aggtactcga tctcctgaag
    16801 gcaaaggatt tttatggttg cccaagacta tacctggcga tgcaaaatgg acatagcgat
    16861 atcgtgaaag ttattctcga agcattgccc tgtctggccc aggaaattaa catttcagct
    16921 tccgatattg tagatcttct gaccgctaaa agtcttgcgc gcgacacggg tttgtttatg
    16981 gccatgcagc gcggacatat gaacgttatt aatactattt ttaacgcatt acccactctt
    17041 tttaatacgt ttaaattcga taaaaaaata tgaagcccct cctcctggca aataattcta
    17101 atgaatatcc cggtttgttt tcagcgatac agcataaaca acaaaacgtt gtagagacgg
    17161 tttatcttgc tttatctgac catgcacgcc tgtttggatt taccgctgaa gatattatgg
    17221 atttttggca acacaaagcg ccacaaaaat actctgcctt tgagttggct tttgaatttg
    17281 gtcaccgggt tattgctgaa ttaatcctta atacattaaa taagatggct gaaagctttg
    17341 gctttacgga taaccctcga tacattgcgg agaaaaatta tatggaagct ttactcaaaa
    17401 aagcatctcc ccataccgta cgctaaatct ttgcctgcgc cgatacgcat aaacatcttc
    17461 cacatgccct tcacgtatgc ggttttgtag tgcgcgccag taatcagcgc ggaacaggtc
    17521 ggcgtgcatc tcttcaaaca gcggaccaat acgcgggtcg gcgcatagcc agtggcgaaa
    17581 ctcttccggg aaaacatcgc ccggcgagac gctgtaccac ggttcgctgg caagttcgtc
    17641 ttccggatag cgcggcggcg ggatgtcgcg gaaattcact tccgtcatgt agcaaatttc
    17701 atcgtaatca taaaaaacca cacgcccgtg acgggtgaca ccgaagtttt taaacagcat
    17761 gtcgccaggg aaaatgttag cagcggcaag ctggcgaata gcgttaccgt attcttcaat
    17821 ggcgtcgcgc aactgctgac cttccacttg ttccagccag atattgagcg gcaccatccg
    17881 ccgctcgatt atagagatgg cgaattacaa tctgctcacc aagatcggtg attttttccg
    17941 ctgcttcctg gagcagtaat tccattaata ccggggaaat atgccgcttc tccagcacaa
    18001 agttttcaaa ctcctgggtg tccgccattc ggcccacacg gtcgtgctct ttcaccagtt
    18061 gatagcaggc acgaacgtga gcggcagaca tctctttctg cggcgcgaac ttgtctttga
    18121 tgactttgaa tacccgatca aagcccggca gcgtaaacac caacatcacc attccacgaa
    18181 tacccggcgc ttcaatgaac tgctcattac agccctgtag ataaacgaga tattcgcggt
    18241 agctttcggt tttggcgtgc ttctggcagc cgatagccat atacaattca gcagtggttt
    18301 tacctggcag aatttcccgt agccactcga ccagcgctgc gggcagcggt gcataaacca
    18361 taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg
    18421 tatcaataaa taactcgccg tcgtccgtct ggtggatcgg cagtaagaag ggcaacgtgc
    18481 cggaaggcgt gatcagtttg cccaccagcc aggcggcttt attgcggtaa aacagttcgt
    18541 tcgccacctg taaatgactt tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc
    18601 gaatgatgta atgaatgtca cggcttttat tctgccagcg caggcgcagc ggcaggtcgc
    18661 tgataacgcg catcagtaga gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg
    18721 ggcggggaat ggtacgaaag cggcgctctg gctgagagct aaagataaaa agccgctcgg
    18781 gagtaagcga gcggtggtca aataaccgac agtacacgga gttaaaaaag ctctccgcaa
    18841 tctcgaagcg cgggtaatcc ggcaacagcc gcgtgtaatg ctctttaacg cgcagtaaaa
    18901 atgccgcgtc cgtactttgg ccgttagtaa tgcagcgcag ttgctccacg accagaccaa
    18961 cgtgatgatc gtaaagatgg atacggtttt tcatcgcctg ctggacagca tgccagtcgg
    19021 cctgttcgaa acgctgctgc gccccggaag tcacttcgag gaatcgacca tactgagcgt
    19081 cgaagccttg caaaatggtt tgagcaatca gtaattccag gccacgcggc atttagcgcc
    19141 ctcatcagga gcagagaatt gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg
    19201 caacggttgt aggcatgata agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc
    19261 cggatgcggc gtaaatgcct tatccggcct acagtcagca acgtttgtag gttgcttaga
    19321 actgcgattc ttcagtggag ccagtcagcg cggtgactga agacgtgccg ccctgaataa
    19381 tagtcgtcac tttatcgaag taacctgtac ccacttcctg ctggtgagat acgaaggtat
    19441 agccatcttt cgcggcggca aattccggct gctgcacttt ctcaacatag tgcttcatac
    19501 cttcgccctg cgcataagcg tttgccaggt cgaacatgtt aaaccacatg ctgtggatac
    19561 ctgctagggt gatgaactgg aacttgtagc ccatatccga cagctgctgc tggaagctgg
    19621 caatagtttt gtcgtcgagg tttttctgcc agttgaacga cggcgagcag ttataagcca
    19681 gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa gcgacgcgcc agttccagat
    19741 ccggcgtgga ggtttcacac cagaccaggt cagcatatgg cgcatacgcc aggccacggc
    19801 tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc ttcactggta cgctcgccgg
    19861 taataaattc gctgtcatac gggtcgcaat ctgaggtgat cagatccgcc gcatcagcat
    19921 cggtacgtgc aaccagcagg gttggaacgc ccgtcacgtc agctgccaga cgcgccgcga
    19981 ccagtttctg aatagcttcc tgagttggca ctaaaacttt gccgcccatg tgaccgcatt
    20041 tcttcactga cgccagctga tcttcgaagt gaactgccgc agcaccggct tcaatcatcg
    20101 ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca tcggcaacga
    20161 tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcccacg gaccattgga
    20221 tctgatcggc acgacggaag gtgttgttga tccgctccac cacagctggc accgagtttg
    20281 ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc gccgctacct
    20341 gccatcccga cagatagact gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc
    20401 cagtcagtgc accgaggttg ttgatgtagc cttttttcga ctcaccgtgc agcagacgcc
    20461 acattttcgc tgcgcccagt tgcgccagcg tgcattcagg attgactgaa ccgcgtaatt
    20521 tcaccacatc ttccgcactg tatgggcgag taatgccttc ccaacgcggt tgagtccact
    20581 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
    20641 tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc
    20701 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca aaacggccct gggagaaacg
    20761 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
    20821 ggctttggtc accggtttgc cattgctcaa cgttttttga tgatggatcc actgccagat
    20881 cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacgca
    20941 gccgttgccg gagatccacg cttcgatgta ctgcacagcc acgcgaatgt tagcgcgcat
    21001 accttcttcg gtgcgttcac catcacaagg tgccagcagc tgatcggcag taatcggcgc
    21061 gtcttgttcg cgcaatactt caagctgatt tttacgggag ccgagaatgt cgttgaatac
    21121 cgccattgcc gtgtccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt
    21181 ggcttccagc gctttatccg cttttacttt gttgagcacc tggttattac gctcttcatc
    21241 tttgctcgga ataaacgcgg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
    21301 tttaatcagc aggcgtgagt aagcattcag gaatggttta tccatcgtca cggcctgtct
    21361 gtctggcagg acacgatcgg gatagttttt caacgttttg atatagctga agatgtaatc
    21421 ccagcgaccg cagttcagac caacaatatg gtcgcgcagc gcgtgaagga tttcgtccat
    21481 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
    21541 attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt cctgctagga
    21601 ctgggttttc ggcagataga aatagggacc gctgcccttt gccaacagtg cctgatagtt
    21661 gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg attgcctcac cacgccaggt
    21721 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
    21781 cttgagctga taaattttgc ctgcttcatt ggtatagctg atggtaccgt taaccgcatc
    21841 gcgcaggtta atttgcccgt cgatcacttt gttccagtct ggtgccagtg aatcttcgaa
    21901 atcggccata aagactttca cattggcgtt cagtgcgttg atcaccatct tgcgctctac
    21961 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
    22021 atcagcatcg cgaatggaag ccgtttccga aataaaatca ggcaacgttc cgttatcaat
    22081 atcttgctgc tgctgaatgc gcgctgccag aagtttattg cgttgtggcg taaaatgcgt
    22141 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
    22201 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
    22261 cctcgtcatg gatccgaaaa cttcccctct gtgaacgaag gaacgttgtg cgcttttcgt
    22321 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt
    22381 aaaaatactt gataacttat tgataacaat atgaataaaa tttattcact tgctgaggtg
    22441 cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga atcgcttaac
    22501 gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga tcgtatggcg
    22561 tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg tgactacgcc
    22621 agctcgctcg cggtttgttt tgcggatcgt tgtgcgggaa atagttatac ggtacatccg
    22681 ggtctagtcc ggcttccaca tcgcgtaaat attcctgcgc cagcgtttgc gcatcatatt
    22741 cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga tatgcatccc
    22801 cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac gctgccggaa
    22861 agtcagcata gcgcgaatgc ggtgccagga atgaatcatc aaagccacgc gtcagaagcg
    22921 catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct
    22981 taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca aacagcgtcg
    23041 aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag taagcgacat
    23101 cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca aagttctgtt
    23161 cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg
    23221 attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag tttgaaagca
    23281 ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg atcagaacct
    23341 taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag acgttttctt
    23401 cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata acctgattac
    23461 ctcactacat acgcttatac gtttagacat ccagatagct gaagataacc agaaaatttg
    23521 ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaaggct acgttagaat
    23581 ataagaatga cgaaaaggag agaaaatggt tattagtatt cgccgctcac ggcatgagga
    23641 aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacccacg attttttatc
    23701 agcagagtat cgggccgagc tggaagagct ggtgcgttcc tttctgccgg aagcaccgtt
    23761 gtgggtcgcg gttaatgaac gggagcagcc ggttgggttt atgttgttaa gtgggcagca
    23821 tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc ggcgtgggtc ggatgctggt
    23881 ggagcatgcg ctctcgatgg caccggaact gacaaccaac gttaatgagc aaaatgagca
    23941 ggcggttggg ttctataaga aggtgggttt taaggttacg ggacgctctg aggtggacga
    24001 tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg ggggaataag ccgcctctgc
    24061 cgtgcctgcc caagctggag aagacg
//
LOCUS       DAZSNG010000056        23931 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZSNG010000056 DAZSNG010000000
VERSION     DAZSNG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23931
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.057"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..386)
                     /locus_tag="ACHW4Z_002349"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHW4Z_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHW4Z_002350"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHW4Z_002350"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR8881962.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHW4Z_002351"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHW4Z_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR8881963.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVNLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHW4Z_002352"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHW4Z_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR8881964.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHW4Z_002353"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHW4Z_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR8881965.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHW4Z_002354"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHW4Z_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR8881966.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHW4Z_002355"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHW4Z_002355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR8881967.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHW4Z_002356"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHW4Z_002356"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR8881968.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHW4Z_002357"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHW4Z_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR8881969.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHW4Z_002358"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHW4Z_002358"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR8881970.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHW4Z_002359"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHW4Z_002359"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR8881971.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHW4Z_002360"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHW4Z_002360"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR8881972.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHW4Z_002361"
     CDS             10748..11212
                     /locus_tag="ACHW4Z_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR8881973.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHW4Z_002362"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHW4Z_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR8881974.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHW4Z_002363"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHW4Z_002363"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR8881975.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHW4Z_002364"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHW4Z_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR8881976.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHW4Z_002365"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHW4Z_002365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR8881977.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHW4Z_002366"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHW4Z_002366"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR8881978.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHW4Z_002367"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHW4Z_002367"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR8881979.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHW4Z_002368"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHW4Z_002368"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR8881980.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHW4Z_002369"
     CDS             complement(20505..20798)
                     /locus_tag="ACHW4Z_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8881981.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHW4Z_002370"
     CDS             complement(20795..22084)
                     /locus_tag="ACHW4Z_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8881982.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHW4Z_002371"
     CDS             complement(22140..23078)
                     /locus_tag="ACHW4Z_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR8881983.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHW4Z_002372"
     CDS             complement(23098..23862)
                     /locus_tag="ACHW4Z_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR8881984.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5911 a         5804 c         6300 g         5916 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgaatctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg c
//
LOCUS       DAZSNG010000057        23645 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZSNG010000057 DAZSNG010000000
VERSION     DAZSNG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.058"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..841)
                     /gene="focB"
                     /locus_tag="ACHW4Z_002373"
                     /pseudo
     CDS             complement(1..841)
                     /gene="focB"
                     /locus_tag="ACHW4Z_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(863..2875)
                     /gene="hyfR"
                     /locus_tag="ACHW4Z_002374"
                     /pseudo
     CDS             complement(863..2875)
                     /gene="hyfR"
                     /locus_tag="ACHW4Z_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(2905..3318)
                     /gene="hyfJ"
                     /locus_tag="ACHW4Z_002375"
     CDS             complement(2905..3318)
                     /gene="hyfJ"
                     /locus_tag="ACHW4Z_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR8881985.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(3311..4069)
                     /gene="hyfI"
                     /locus_tag="ACHW4Z_002376"
     CDS             complement(3311..4069)
                     /gene="hyfI"
                     /locus_tag="ACHW4Z_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR8881986.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4066..4611)
                     /gene="hyfH"
                     /locus_tag="ACHW4Z_002377"
     CDS             complement(4066..4611)
                     /gene="hyfH"
                     /locus_tag="ACHW4Z_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR8881987.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(4621..6335)
                     /gene="hyfG"
                     /locus_tag="ACHW4Z_002378"
                     /pseudo
     CDS             complement(4621..6335)
                     /gene="hyfG"
                     /locus_tag="ACHW4Z_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(6325..7911)
                     /gene="hyfF"
                     /locus_tag="ACHW4Z_002379"
     CDS             complement(6325..7911)
                     /gene="hyfF"
                     /locus_tag="ACHW4Z_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR8881988.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(7916..8566)
                     /gene="hyfE"
                     /locus_tag="ACHW4Z_002380"
     CDS             complement(7916..8566)
                     /gene="hyfE"
                     /locus_tag="ACHW4Z_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR8881989.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8578..10017)
                     /gene="hyfD"
                     /locus_tag="ACHW4Z_002381"
     CDS             complement(8578..10017)
                     /gene="hyfD"
                     /locus_tag="ACHW4Z_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR8881990.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10034..10981)
                     /gene="hyfC"
                     /locus_tag="ACHW4Z_002382"
     CDS             complement(10034..10981)
                     /gene="hyfC"
                     /locus_tag="ACHW4Z_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR8881991.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10992..13009)
                     /gene="hyfB"
                     /locus_tag="ACHW4Z_002383"
                     /pseudo
     CDS             complement(10992..13009)
                     /gene="hyfB"
                     /locus_tag="ACHW4Z_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13009..13626)
                     /gene="hyfA"
                     /locus_tag="ACHW4Z_002384"
     CDS             complement(13009..13626)
                     /gene="hyfA"
                     /locus_tag="ACHW4Z_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR8881992.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            complement(13879..14349)
                     /gene="bcp"
                     /locus_tag="ACHW4Z_002385"
     CDS             complement(13879..14349)
                     /gene="bcp"
                     /locus_tag="ACHW4Z_002385"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR8881993.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14349..14921)
                     /locus_tag="ACHW4Z_002386"
     CDS             complement(14349..14921)
                     /locus_tag="ACHW4Z_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR8881994.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15067..15945
                     /gene="dapA"
                     /locus_tag="ACHW4Z_002387"
     CDS             15067..15945
                     /gene="dapA"
                     /locus_tag="ACHW4Z_002387"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR8881995.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15962..16996
                     /gene="bamC"
                     /locus_tag="ACHW4Z_002388"
     CDS             15962..16996
                     /gene="bamC"
                     /locus_tag="ACHW4Z_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR8881996.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17209..17922
                     /gene="purC"
                     /locus_tag="ACHW4Z_002389"
     CDS             17209..17922
                     /gene="purC"
                     /locus_tag="ACHW4Z_002389"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR8881997.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18090..18953
                     /locus_tag="ACHW4Z_002390"
     CDS             18090..18953
                     /locus_tag="ACHW4Z_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR8881998.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            18968..20983
                     /gene="tmcA"
                     /locus_tag="ACHW4Z_002391"
     CDS             18968..20983
                     /gene="tmcA"
                     /locus_tag="ACHW4Z_002391"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR8881999.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            21057..21755
                     /gene="ypfH"
                     /locus_tag="ACHW4Z_002392"
     CDS             21057..21755
                     /gene="ypfH"
                     /locus_tag="ACHW4Z_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8882000.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(21865..22065)
                     /locus_tag="ACHW4Z_002393"
     CDS             complement(21865..22065)
                     /locus_tag="ACHW4Z_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR8882001.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22093..23220)
                     /gene="dapE"
                     /locus_tag="ACHW4Z_002394"
     CDS             complement(22093..23220)
                     /gene="dapE"
                     /locus_tag="ACHW4Z_002394"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR8882002.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23224..23580)
                     /locus_tag="ACHW4Z_002395"
     CDS             complement(23224..23580)
                     /locus_tag="ACHW4Z_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR8882003.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5948 a         6254 c         6322 g         5121 t
ORIGIN      
        1 aacaaatact caccagcact gcaccgccga taatattacc cagcatcacc gggagcagat
       61 tggcggtaat aaaatggctg accgtcagtg ccggaaaatt gtctgcgcta ctgtgcgcca
      121 gttgccagaa ggaggtaggg gcgaaatggc gaatggcaat ggcgaacgga atcacaaaca
      181 aattggcgat acagtgctca aagccactgg cgacaaacag ggtgatggac aaaatcatgg
      241 cgacgatttt gtcgcataac gaacgtccgc aataactcat ccacagcgcc aggcaaacca
      301 tcagattgca cataatgccg aggctgacag attcagtaaa tgtatgatgc attttgccct
      361 cggcgcagtg taaaatcgcg actccccaca tcgcgttttc actcatcacc agcccggaaa
      421 accagattaa caaactgaaa caggcaatac ctgccagatt accgcaggcc accagaagtg
      481 cgttaatcag ccaagttcgc caactaataa cgccccgact ttttgccatt accgtcatta
      541 ccgacgaggt gaacaggctg gtgccgcaaa ccgccagcaa aataaagcca agtgtaaagc
      601 aaaggccgcc caccagatgg gttaatgcct atgaagacgg ggcatcggcg ataacggaaa
      661 ggtaaaaagt aaatccgatc gccataaaca cgccagcgga catcgccata aacacgccag
      721 cggacatcgc cattaaaaag accgacactt tactgcgggc aattttatgg gcggcaatcc
      781 gttcagcgat tgccgctttt ctggcgctca actgcaagtc gaaagagagt ttgtttctca
      841 tgcatactcc tgcaaaagca gattacaaca cctcgcgaac cgagattccc agacgctgca
      901 ttcgtgacag cagcgtggtg cgcttcatcc ctaatcgtgt cgccgcgcca cggggcccgg
      961 caacaatgcc attggtttct cgcaatacct gaacaatgcg ctgacgttct tcttcgtcat
     1021 tctctggcgt cgtcgggtgc agcaactacg ccattgaact gcgaagcgct gaatcttcat
     1081 ttagcgtcgg cagtaaacgg ctttgtcgga cattaagatg taaattcaga ctgttaccgc
     1141 gagtcagcag taccgctcgc tcaatcacgt tttccagctc gcgcacgttg cccggccagt
     1201 cccacgacat caactggcgt agcgcctcgg tcgggatggc gtcaattgaa cgattcatat
     1261 ggcgcgccat tttttgcgtg aaatgctttg ctaaaagagg gatatcttcc ggacggtcgc
     1321 gcagcggagg caattccagt gggaagacat tcaggcgata aaagagatcg ctgcgaaact
     1381 ggcgatcttc aaccatttgc cacaaatcac ggttggtggc ggcaatgact ctgacattta
     1441 ccgggatcgt tctactcccg ccgagacgct caatctcccg ttcctgcaat acgcgcagca
     1501 gtttaggctg aagttctaac ggcagatcgc caatttcatc gagaaacaac gtgccgccat
     1561 cggcaatttc aaaacgacca cgatgggtat taatcgcacc agtaaacgcc cctttgtcat
     1621 gaccgaataa ctcactttcc agaagactgg cggggatggc agcgcagttg attttgacca
     1681 gcggcttgtc gtgtcgcggg ctaagttgat ggatcgctct ggcgatcacc tctttgccgg
     1741 ttccggtttc accgcaaatc aacaccgtac tgtcgctctt cgccacaata tctacctgct
     1801 ggagcaggtc ttccattgcc tggctttgat agataatgtc accgacgccc agattcgaaa
     1861 ggagctgctc gctaagctgg tggttttctt gctgcaaact ttcctgcaaa tcggtcattc
     1921 tacggcaggc atcggcattg ccaacggcaa tagcgatgcg atcggctatg tgttgtagta
     1981 gctggcagtt ttcctcacta aagagagtgg aagaggtatg cgccagcaac aatgcacccg
     2041 gtgtatggtt gccaaaggta agcggtataa ggagcgcaga ttcgcagccg ttagatgcaa
     2101 gtaagagtaa taacgggtcg cgttgccaga gaaacagatc atctgcacgg tgcgtcaagg
     2161 tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact gtcgccaggc atattgtgtg
     2221 gcagacacgc acaatgtgag gcagaaagat cgctgcacca caggctgaac ttctcgttct
     2281 ttcgatgatc gcccagtacc atactgactg aagccagacc gaaaaaatga tggatctcac
     2341 gagcgacgtc agcgatcaga tcgtcgagat caagatgtga cagcactgca ttagtgatat
     2401 ccaccagtac ccgatgatga tcgcgatctt tctccagcaa acgcagcgcc acctgctgtg
     2461 cttcatcatt gagccatgat tgcagaaccg gggtgataat gcgtgccagc cagttaaaca
     2521 gtgtgcaatc ctggggagta aaacggctgc cgtgggtacg aataaagcgt agctccgcca
     2581 ttccactact gccgagaagg acggcgtaac gcgaataatc gaccatctcg ccctgatact
     2641 ctgttgttgc cgtcacatcg ctgccatcat catggtagcg gtaatagaag ttgcttttga
     2701 gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg cggcagcggg atgaaggcgc
     2761 gtaataaaga cgccttgccg tgtttctgcg ctaaccgctc cgcgagcatt ccgtttaccg
     2821 cttcaattgt tattccttgc ggcggggcaa acatcgcttc gtctgacata gccatcaaca
     2881 gtccttatta atgagaaatc cttctcacgc aatttttctg gccatcagat agatagccgg
     2941 ttccagcaca atttcatcca acagatgaat gaattgaatg ctacgcgctt gccacacaga
     3001 cgtcgcgtca tctgccagtg gcgcgaatgc ccgggtcaac agggcggtgt ggctggcgtc
     3061 aataacaatc tcaccaaaag tcatcacccc cagcatctta cgtcgggctt gctcctcttc
     3121 gaccagtgca agccaatctt cgtattccgt cagtgggcag cggaaggcga cattcagaca
     3181 atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa tacattacct gagagctgtg
     3241 ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc agcgtccaga aaacaatttc
     3301 cccgcactct tcagtcatgt aacccccgga gtacgcgaaa gagttgctga acgatactat
     3361 tgagacgtgg atcgtcggca tcgcgcaacc aggtgttaac ccgatttcct gtaggatcgt
     3421 cgcttaaatg gcgcaggagc cgatcgcaaa tttctcgccc ctgacgatag cccgccagcc
     3481 gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg gatctgcggc cacaacggtt
     3541 gcatagtcac cccggtggga tcgcgataat ccaccgcgtg aatcttctgt tgcagcaaac
     3601 cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt tggcgggcag ccggggatcc
     3661 aaacatcaat ggggacaatg gtgtcgctgc cgccccagac gctgtagaga tcgtggaaaa
     3721 taccgccgcc gacaccgcac gcgccgtagg aaacacagat tttatgatcg ggggcagact
     3781 catacgcccg aagtgcaggc atacgcatcg cccgggtgac tgcgccagta aataacaaaa
     3841 tatcggcgtg acgcggtgat gaaacaacct taatgccaaa acgttctgcg tcgaatacgg
     3901 gggtgatggc agcaaaaatt tcgatttcac aggcgttgca gccgccgcaa tcgatgcgat
     3961 aaacgtaagc cgagcgacgg atatcctgta gcaaatgccg cttcatcttt tgcgtttgct
     4021 cgtccagcgt gatgggctgg ctgacatatt gcgtaagcac tggactcata gctgctcctt
     4081 agccaccagc ggtacatctg tatcgtcgtt gatcagcgtt gcgcgttgtt tgcactccgg
     4141 gcagacgctc gcttgcgccc ataacatttc gcggttttgt ggagcatttt gttgctgtgc
     4201 caacaattca gcagccagtg cgacggtttt ttgcggggca aacgggcgtt cgcaacggct
     4261 gcaacgttgt agatggaacg tcgcgtgggt atagagatcg gctttatggg tgacggtcag
     4321 ttcaaagtta ttggtaagct ggatggctct ggtcgggcac acttcttcac aacgtccgca
     4381 gtaaatacaa cgccccagat agagctgcca ggtgcgcgaa ttttgctggt cgtcggtctg
     4441 gatagtcagc gcatttgccg gacaagcaca ggcgcaggca ccgcaggcaa tacattggct
     4501 gggcatcagg tccggtttcc cgcgaaagcc tgggctgacc tccagtggcg cgaagggata
     4561 tttcaccgtc gcggttccgg cgcgcataat agttttcagt aacttcagca tcttctgtcc
     4621 ttacttcagc ggcgaacggt tacgatcaat gccgtagcgt tcgatctctt tatacagcac
     4681 ggtttttgac tggcgcttgc gcacatctac cagcgtcaca cggtcggtac aggagtagca
     4741 gggatcaagg ctaccgataa tcagcggtgc gtcagaaacg gtattgccgc gcagcatgta
     4801 acgcaacacc ggccagttgg cgtaggtggc ggcacgacag cgccagcgga acaatttttg
     4861 gttatcaccg agcatgctcc agtgcacgtc ttcaccgcgt ggcgcttcaa caaagcccag
     4921 cgcgaatgcg tgaggtttat agctaaagcc ttcggtcagc agtggggtat ccggcatgtt
     4981 gtcgagggca aattccagca ttgccagcga atcaaacgtc tctttgacac ggaccatcac
     5041 gcgggagaaa acatcgccgc cggtaaaggt aaacagtgtt tttggaatat tgccgtagtc
     5101 ggcgtagggg tgatcaaagc gcaaatcacg ggcaaaacca ctgcgcggat cagcggccct
     5161 acagggctat aatcacgggc gatttgtcgg tcgagaatgc caatgccctg agtgcgttgt
     5221 tccatattcg gcgtagcaag cagcatctct accagctccg acacgtcggc gcgcatctcg
     5281 cgcaccagtt tcagcgtttg cagacgttgc tctttgagaa tatcgcggcg aacaccacca
     5341 atcagattca gaccgtaggt tttacgcgac ccggtcagca attccgccat cgtcatcgac
     5401 ttttcccgca cgcggaaaaa ttgcataaag ccggtatcaa aaccaacaaa atggcaggag
     5461 aggccaaggt tgagcaaatg actgtgtagc cgctcgactt ccagcagaat cgagcgaata
     5521 gtatgtgctc gttgcggcac ctcaatcccc agtgcatttt caaccgagtt ggtataggcc
     5581 acactgtggg caaaaccgca aatcccacac acgcggtccg ataagaaggt cacttcgttg
     5641 tagcccatcc gcgtttctgc cagtttctcc atgccgcgat ggacataaaa caggcggtaa
     5701 tcagcatcga caatttgctc gccatccaca aacaagcgga agtgacccgg ttcataggag
     5761 gtgatatgca gcgggccgac agggatcacc cgcgcatcgc tgttgccctc attgataaac
     5821 gcatacgttt cggaatctgt cgtcggttca gggcgcagtc gataatccat cgcatctttg
     5881 cgcagcggat gcatatcttc cggccagtca tcgggcaaca ccaggcgacg ctgatccggc
     5941 aggccaaccg gaatcagccc gtacatatcg cgaatttctc gctcgcccca gaccgcggca
     6001 gggacgcgcg gtgtgactga cggaaactcc cgactgtcgg catcgaccag tgccttcacc
     6061 acgatccagc atttttcggc cccttccatt gaaagggcat aataaaccgc gtaatgaccg
     6121 ttaagtgtcc gctcgtcgtt gccaaacagg actggaagcc agccatcatg ttgataataa
     6181 agataatgta caacgtcagg cagcagattg attttcaccg taatggtgac ctgttcaggc
     6241 gtttgtcgct cttcatccag caccgcgccg gggaactgcg ttttcagggc ggcgagaatc
     6301 gcttcgccac gatttgacga tgaattaacg ttcactgtgt ttctccgaaa cagatgcggt
     6361 gcctgaaggc aaaaaatcat gccaggtgct acgttgtgca ggcaggtcgt gcgtccctga
     6421 gaggactata gtggaagcgc ccgccaggat cctgatgaca ggttgtggaa tatgcgttcc
     6481 catcgccagc atcatgacca gcagaatcac cattggcgag gtggtcaacc agccgagatc
     6541 accccggtta acggcctgcg gcggtttcgc cattaacacc cgcgcagcca tccgtaccag
     6601 gcccgccagc accagcgtta acagcaataa cagcaggacg ataatcagca ggtgattacg
     6661 tgccagtcct gcggtaacgg tcataaattc gctaagaaaa atgttgaagg gcggcatccc
     6721 tgccagcgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt
     6781 gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga
     6841 accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa
     6901 aattcccagc ggcccgccaa tgcctagcgc caccgcgacc agccccatgt tctccacgct
     6961 ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc
     7021 aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg
     7081 gcaaataatg atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga
     7141 gagcagggcg ctgaccggac tcggcgcttc actgtgagca tccggcagcc aggcgtgcat
     7201 gggaaatagc ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat
     7261 taatgttggg tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc
     7321 tgcctgcggc ataacgctgg cggcgttggc gtataccagc acggtaccga acagaccaaa
     7381 agcgacacca acagtacaaa taatgatgta cttccatgct gattccagcg atgaacgctg
     7441 accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgatcg ccgcccacat
     7501 cacaatcagg ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa
     7561 accgtagtaa tcgcacagtg taacgggtga aagctcgccg tgtgccactt catgacgcat
     7621 gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa
     7681 accgcccaga ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc
     7741 ggcagtttgg accacccaga gtgctagaat cagcagcagt gtgatcccta agctatgtaa
     7801 tacagtcacc gtacaagtcg cagagagccc ccgtttccgg caggcaaaac agagcagcga
     7861 aaaaagcagc ggcgtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc
     7921 ccttcagcgc ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg
     7981 ccagtaacac catcacaatg acggcgaaga tggcgtcggt agcgatacct atttccacca
     8041 gttccggtgc tcgccaggca agaagcgcca gcaccagatg ggagccgttt tccatcaggc
     8101 agtaaccaaa aatttgccgc aggatattgc gttggctgac aatgcacagc aggccaagca
     8161 gaaaatgacc taacgctacc gccagcgccg gtttcagccc ggtagccatc ggtagcttca
     8221 cgggctgaac gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg
     8281 ccggaccgaa taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca
     8341 tgattaacgg taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt
     8401 gctctgtggc gaacaggcac gagagagtgg cgaaaataga caccagcacc agcgactggc
     8461 aggcgtaaaa tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca
     8521 gcatcatcag tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta
     8581 ctgcaaccag gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcaa
     8641 caccaggcgc attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc
     8701 cggcacgacg cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat
     8761 cagcagaatc atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcggc
     8821 aaacagcggg aatttactga agaagccgtt gaacggcggc acaccggtaa ttgccagcgc
     8881 tgccacgcag aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg
     8941 caacaagcgc gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt
     9001 agcgaacgcg tggttgacga tgtaggcgat gctaccctcc agcgccagcc gcgagccgaa
     9061 gatggagagc gacaagccga agaacatcca gccaagttga gtgatggtcg accaggccag
     9121 caaccgcttc atatcctgct gtggcaaata catcagaaag ccataaagaa tggtgaccag
     9181 tgtcatgacc atgccaacgc cgccaatcac atgcgggata ttgccgccgt cgataatggc
     9241 gcgggcaaaa atgtaaacgc ccactttcac catcgatgcg gcgtggagat aggcgctgat
     9301 cggtgttggc gcttccattg cgtccggtag ccacgcttgc atcggtagct gggccgattt
     9361 cccccacgcg gcaaacagga tgccgccata aaccagataa cgtgcgtcgc catgtaactc
     9421 gctcatcgcg ctaagcacaa acgttccggt ctgcaaaaac agcgtggcgg cggcaagata
     9481 caaccccaac gaaccgatat gggtgataag caacgctttt agtgctgaac gctgcgcttt
     9541 atcgctctgg taataactga tcaacgccca ggagcagccg cccgtaattt caaaaaacaa
     9601 caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaccag
     9661 caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag
     9721 ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag
     9781 cgtactcacg cgatcaatga ccaggccaaa taatgccaca tcccccacgt tgaccaacgt
     9841 caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca acatgcacag
     9901 cgtggtcagc gcggcgaaca aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9961 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
    10021 ccgtcagtgc tccttacaga ccggttaacc aaaagaccca tgcaagcgca gcaaggctga
    10081 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
    10141 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10201 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10261 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10321 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10381 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10441 ttagcggcga gttccagccc atagccagcg tattgctgat catctcgata tgcgtggaac
    10501 ctgctatcag cgccaatacc agcagtgaga gaataagcat tggttcgacc agaatgccga
    10561 gcgtcaactc gcgactggca ccgactcccg caaacggact tccggtatcc agcccggaaa
    10621 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10681 ccgcaaaagg ggaaacggta ataaatagtg gtaaggccat cgccagcacc agcatgctgc
    10741 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcaacttcct
    10801 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10861 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10921 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10981 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    11041 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttgac cagccgttgg
    11101 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    11161 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11221 agacgaaata acgcagagaa gaccacacgc agcggctgca tcacgccatt gcctgacgga
    11281 gccatcgcgt tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11341 gcgcggcgcg aacgacacca cagccagtaa aggcctggca gcaaaggcat cgccagtagt
    11401 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11461 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatatg cattatcttc
    11521 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11581 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca
    11641 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11701 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11761 agtgagaaca gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11821 gctgatatcg cgaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11881 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc gtccgcgccg
    11941 agaaatagca gccctttgaa cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    12001 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccgt ccatcgccac accgactgcc
    12061 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    12121 tccgccagcg catatagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12181 atgccccacc acagaggcaa acccgtttgc gccagcagat ccatcgctac tttcaggatg
    12241 ccgaaaatac cgattttgac cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg
    12301 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12361 ccgaaaaagg ccagcagaaa caccgccgac gccagccccg gagaaagtga aagcgtgcga
    12421 aactggcaaa atcgaggctg ccgctttcgc gccccatcag caagaaggcg atcataatca
    12481 gcaccgatcc ggcgtgggcg ataaaaaagt agagcatgcc agcgttgatc gacgttttat
    12541 cctgcctggc aatgaccaga aaccaggaag acagcgacat catttcaaac agcacgatga
    12601 accaaaaagc gttgtccatc accagcaggg caaccatcga tgcgatgaaa agattcataa
    12661 agaagcccat cgccgccgcg cctttgccct cgtattcgcg catataagtc aatgaataga
    12721 gcgaacaaac caccaccagc aaggagataa ctagcaccat aaatgccgag aggctatcca
    12781 ttcgcagggt taaatcggca aagtcaaaag gggtggcaaa atgggcgact aaagttacac
    12841 cagcatgtaa ttgcgtaatt ccgctgatga tgccaatcac gccgcccacc agcgatgtga
    12901 tgccggaaag cttaatagcc agtctgtcca gaccgagtaa aaacagcgaa gccagactgg
    12961 caaacagata gaggatcagc gaccaggtta ataattgcag ggcatccatt agtgttgctc
    13021 ctgagtgagg gaaaggggag actccccgcc attggcaaac cagcttgcgg caaggcgctg
    13081 tttttctttc atctgacgtt gcaggctatc gtcggtgatc agccgtaacg cctgattcgg
    13141 gcaagcgcga acgcaggccg gaccttctgg caagaaatca cacaggtcgc atttcaccgc
    13201 gacggtctgg acacccggtt cccagcgcag caacgcatgt tggggcggca cgttttcttc
    13261 gccgtctttg agtgagcctt cagcctgaaa aacatattgc gcatgggcat tcaccggacg
    13321 gcttcctgaa gcgctgattg cgccaaatgg gcagaccacg gcgcaaagct tgcagccaat
    13381 acagaggctt tcgttgagtt ggatcgcatc atccctctga gagatggcat tgaccgggca
    13441 gacctgcagg caaggggctt cctcacagtg atgacacacg acaggggcag tgattgttga
    13501 cgtcttcgcc agggccaggc gcgggtgttg ctgtaaacct tgcgttttat gcacgtccga
    13561 acaggcagcg agacaggtat tacatcctgt acaccacagt ggttcggcca ccacaaagcg
    13621 gttcatgtcg gctccttgaa aatgatatga aacattgcat ttcatattgt ataaaggatg
    13681 ccattctgat taactcaatc tattcaataa gttatgtatt aactggattc tgaacggtac
    13741 aaaaatgatg gtcattaacg ttcagcaaaa aatggattcg acacccgtgt cgcaggggaa
    13801 agcgagaatg tgatcttgat cacgagaagt gaggtatgct ggccgcaagc gcagccagca
    13861 cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg
    13921 attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa
    13981 gctgatgcga tgaataccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc
    14041 gaattgttcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt
    14101 ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc
    14161 cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg
    14221 ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt
    14281 aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag
    14341 tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata
    14401 ctgccttgtg cattgagttc tgtacatagg gctttgaacg cttgctcaat atttgctgcg
    14461 tccgcagatg cggggctgtg ggcggttatc tgaatatgca actgcgcagc ccgttcattt
    14521 tcagcaggtt gtgtgcgcga caccagctcc gcaatgttca tatgatgcgc gtcgaaaagt
    14581 gctgtgaagc gttcaattaa atgcggggag tctgcaacat cgacctgaac ccagacagat
    14641 gctggcattg gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagt
    14701 tcggcacctt tcaacggtaa cgttgattca atcagagtga tggcattcca tgaaccggaa
    14761 agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta
    14821 cagccgcaac tactgacatg acgggtgatg gtgttcacaa ttccagggcg atcggcaccc
    14881 aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat tcctttaagg
    14941 ggtgagttgt tcttaaggaa agcataaaaa aatcatgcat acaacaatca gaacggttct
    15001 gtctgcttgc ttttaatgcc ataccaaacg taccattgag acacttgttt gcacagagga
    15061 tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat
    15121 gtctgtcggg ctagcttgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg
    15181 atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat
    15241 gtggtgatga tgacgctgga tctggctgat gggcgcattc cggtaattgc cgggaccggc
    15301 gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag tggtatcgtc
    15361 ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat
    15421 ttcaaagcca tcgctgagca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt
    15481 actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc
    15541 ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca
    15601 gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattgggc
    15661 ggtcatggag ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc
    15721 aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca
    15781 ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa
    15841 ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac cgacagtggt
    15901 cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt
    15961 gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt
    16021 attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc
    16081 ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga ttttgccggt
    16141 gacctccggt gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct
    16201 ggacattcgt ccaccagccc agccgttggc actggtttct ggcgcgcgta cccagttcac
    16261 gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt
    16321 ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac
    16381 actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg
    16441 ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa
    16501 cctggaacag gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga
    16561 gatgatgaac gttatttccg ccggtctgga taaatctgcc actgacgccg cgaacgctgc
    16621 gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc
    16681 aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag cggcgctgga
    16741 aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta
    16801 taagccgctg tctgacagcg actggcagga actgggcgcg agcgatccag gcctggcatc
    16861 cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga
    16921 tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc
    16981 tgcgtttagc aagtaaaaat acagggctgg aatcatccgg cccttttttc tgatatgata
    17041 cgcaaacgtg tgcgtcggca ggaaaacgcg attttagcgg taattcgcac gaaatttgtt
    17101 tgtcggacgt agttcggata aggcgttcac gccgcatccg acaaaacatc cggcacacca
    17161 gacagcaaaa gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa
    17221 gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg
    17281 gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat
    17341 cgcaaaggta tggtgaacaa caagttcaac tacttcatta tgagcaaact ggctgaagcg
    17401 ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg
    17461 gatatggtgc cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct agtgaaacgt
    17521 cttggaatcg aagaaggtat tgagctgaac ccgccgctgt tcgatctgtt cctgaaaaac
    17581 gatgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtgagc
    17641 aaagagaacc tggcgcgtat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa
    17701 ctgttcgatg atgctggtct gattctggtc gacttcaagc tggagtttgg tctgtacaaa
    17761 ggtgaagtgg tactgggcga tgagttctcc ccggacggca gccgcctgtg ggacaaagaa
    17821 acactggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct gatcgaagcc
    17881 tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg ttcatcatct
    17941 tgccgtgcag ctggcacggc aagacaaccg ctcttggaat atatgcattg aacgctcatt
    18001 cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt
    18061 tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca
    18121 atgttgaaga caggcgcaac agctctggcg gtccgtcaat gggcggtccc ggtttccgtc
    18181 tgccgagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg
    18241 gcgttgattt aaccgggttg atgaccgggc agccggtttc tcaacaacaa tcaacgcggt
    18301 caattagccc taatgaagac gaagccgcaa aattcacctc ggtgattcag gcaaccacgg
    18361 aagacacctg gggacaacag ttcgaaaaga tgggtaagac ctatcagcaa ccgaagctgg
    18421 tcatgtaccg tggaatgacg cgcaccggct gcggggcggg ccagtccatt atggggccgt
    18481 tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat gacatgaaag
    18541 acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc
    18601 atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga
    18661 cgcaggcgga agtaaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg
    18721 gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aactggcgat ctggaagagg
    18781 cgctgaatgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    18841 tagtaccaga cagtttcaca catggcactt ctcagcaacg ctacagctgg tttaaacgtg
    18901 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taatttccgg
    18961 ggcagggatg gctgaactga ctgcgcttta cacattaaca gcgcaaatga aacgtgaagg
    19021 gatctgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgctcttaa
    19081 gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagctag atgctgaaaa
    19141 ccactgttca ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt
    19201 cgacgcccgc cacggctttg atgccgctgc ctttgcagca cttagcggaa cgttgaaagc
    19261 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    19321 cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    19381 tctcaaacgc gtacttacgg cggataacga cgctatcctc tggcggcaaa accagccgtt
    19441 ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg caccacaacc
    19501 agaacaacag caactcttac agcagctact gaccatgccg ccgggcgtgg cagcggtaac
    19561 ggctgcgcgt gggcgcggta agtcggcgtt ggcagggcaa ctcatttctc gtattgcggg
    19621 cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg
    19681 cgagaagttt cgctttattg caccggatgc cttgttagcc agcgatgagc aagccgactg
    19741 gctggtggtc gacgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    19801 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggtaccg gacgtggttt
    19861 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat
    19921 ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttaacga
    19981 tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg aacaaacgtt
    20041 atggcgaagc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta
    20101 ccggacctcg ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca
    20161 ggcggctggc gaaaacgaga ttgccggggc gttgtggctg gtggatgagg gcggattatc
    20221 tcaagaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    20281 ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gaaggcgggt
    20341 tagccggata gcagttcatc cggctcgtca gcgggaaggc acagggcgac aacttattgc
    20401 tggagctttg caatatacgc atgacctcga ctatctttcg gtgagttttg gttacactgg
    20461 ggagttatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcggatgg gtaatcatcg
    20521 ggaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg atgcgggtaa
    20581 acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg
    20641 gaatggcgaa atgcttcctg ttgatccact aaacgatgcc atcctttctg acgacgactg
    20701 gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacatcgt taggttgctt
    20761 attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc gtttacagaa
    20821 aaacgccagt gacgcgcagt tatgtaccac acttaaactt tcaggccgca agatgttact
    20881 ggttcgccag cgggaagagg ccgcgcaggc gctgttcgca ctaaatgagg ttcgcactga
    20941 gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    21001 catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    21061 aacatgacca ttttgttgtt caaagcccgg ataaacctgc acaacagttg ttactgcttt
    21121 tccacggtgt cggggataac ccggtggcaa tgggggaaat aggcagctgg tttgcaccgc
    21181 tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt aatccggcgg
    21241 ggcgtcagtg gttttcagtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    21301 ccatcatgcc gacgtttatt gaaacggtac gctactggca gaaacagagc ggagtagggg
    21361 ctaacgccac ggcgctaatt ggtttctcac aaggcgcgat tatggcgctg gagagcatta
    21421 aagccgaacc gggccttgct tcgcgcgtca ttgcctttaa cggacgctat gcgagcctgc
    21481 cggagaccgc gtctacagcc acgacaattc acttgattca tggtggtgaa gatccggtta
    21541 tcgatctggc gcatgcagtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga
    21601 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgccc
    21661 tggatcactt gcgttatacc attccgaaac attatttcga ccaagcgcta agtggcggta
    21721 agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg gcctgataag
    21781 acgcgtgagc gtcgcatcag ggatcgttca ccattgccgg atgcggcgtt aacgccttat
    21841 ccggcctacg gttgagcact cagcttactt cttcggttga tcctttttcg gccagtcgtc
    21901 gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag
    21961 aaatttctta tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctaccaa
    22021 aaagacaatc accagaatcc accaatattt agccagccag tccatgctta tttcctcttg
    22081 cagaaccact catcacgcga cgagctgttc catgatacgt tgatacatac gggcaagtag
    22141 ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat tgaccggccc
    22201 gagttccacc acctgcgccc ccatgcgagc aataaagcgc ccgtcggacg ttccgcctgt
    22261 ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac
    22321 cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccaatcca ccgtatagcg
    22381 cagttgatgt ttttcaagca gggcaagtac ctgcgctttg atcatctcat cagtcagttc
    22441 ggtgctgaag cggaagttaa actgcacaaa cagttcaccc ggaataacat tgttactgcc
    22501 cgttcccgcc tgaatattgg caatctgcat actggtcgcc gggaagaatt cattgccctg
    22561 atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta ccggattgtc
    22621 agccagatgt gggtaggcaa catgcccctg aacgccatga atggtgaggt tgcaggttaa
    22681 cgatccgcga cgaccatttt tcaccacatc acctaccact tcgatactcg acggttcgcc
    22741 gaccaggcag taatcgagac gctcattacg tgccattaac gcctcgacga cttttaccgt
    22801 accgttgtgg gcactggctt cttcatcaga agtgatcaga aatgccagtc gccccgtatg
    22861 gttgggatgt tgtgcgacaa aacgttctgc tgctaccacc atcgccgcca gcgagccttt
    22921 catatctgcc gcaccgcgcc cgaataacat gccgtcacga atggtgggtt caaacggcgg
    22981 attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggctaa
    23041 cgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaaat ccatgcgttc
    23101 aacggtaaaa ccgatcgcct gcaaacgttc aatcaacaaa gcctgacatc ctgcgtcatc
    23161 aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat
    23221 agactacacc tcatggaaaa attgctgata actggaatca ctgaaaccca gcagcatagg
    23281 cttaccaggc gcgcagagca atggacgttt gataattgca ggcatttcag tcattaatgc
    23341 cgccgcagag gccgcatcgg tgattttatt gcgggtagtt tcgtccagtt tacgccaggt
    23401 tgtaccacgg gtgttgagta acgcttccca gcctaattcg ttgataaaac cgttcaataa
    23461 ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt tattggcttc
    23521 cagccaacgg cgagcctttt taatggtgtc acaatttttg atgccgtaaa gtgtaaccat
    23581 aatgaatcct ttttcgattt atgagaataa atactcattt aagggtgaaa acattttgcc
    23641 tgcaa
//
LOCUS       DAZSNG010000058        22941 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZSNG010000058 DAZSNG010000000
VERSION     DAZSNG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22941
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.059"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            92..868
                     /gene="exuR"
                     /locus_tag="ACHW4Z_002396"
     CDS             92..868
                     /gene="exuR"
                     /locus_tag="ACHW4Z_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR8882004.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1108..1191
                     /gene="yqiM"
                     /locus_tag="ACHW4Z_002397"
     CDS             1108..1191
                     /gene="yqiM"
                     /locus_tag="ACHW4Z_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR8882005.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1213..1875
                     /gene="yqjA"
                     /locus_tag="ACHW4Z_002398"
     CDS             1213..1875
                     /gene="yqjA"
                     /locus_tag="ACHW4Z_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR8882006.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1879..2262
                     /gene="mzrA"
                     /locus_tag="ACHW4Z_002399"
     CDS             1879..2262
                     /gene="mzrA"
                     /locus_tag="ACHW4Z_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR8882007.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2409..2763
                     /gene="yqjC"
                     /locus_tag="ACHW4Z_002400"
                     /pseudo
     CDS             2409..2763
                     /gene="yqjC"
                     /locus_tag="ACHW4Z_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2801..3106
                     /locus_tag="ACHW4Z_002401"
     CDS             2801..3106
                     /locus_tag="ACHW4Z_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR8882008.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3109..3513
                     /locus_tag="ACHW4Z_002402"
     CDS             3109..3513
                     /locus_tag="ACHW4Z_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR8882009.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3503..3802
                     /locus_tag="ACHW4Z_002403"
     CDS             3503..3802
                     /locus_tag="ACHW4Z_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR8882010.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3988..4284
                     /locus_tag="ACHW4Z_002404"
     CDS             3988..4284
                     /locus_tag="ACHW4Z_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8882011.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4354..5340
                     /gene="yqjG"
                     /locus_tag="ACHW4Z_002405"
     CDS             4354..5340
                     /gene="yqjG"
                     /locus_tag="ACHW4Z_002405"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR8882012.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5633..6001
                     /locus_tag="ACHW4Z_002406"
     CDS             5633..6001
                     /locus_tag="ACHW4Z_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR8882013.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6208..7104)
                     /gene="yhaJ"
                     /locus_tag="ACHW4Z_002407"
     CDS             complement(6208..7104)
                     /gene="yhaJ"
                     /locus_tag="ACHW4Z_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR8882014.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7209..7910
                     /locus_tag="ACHW4Z_002408"
     CDS             7209..7910
                     /locus_tag="ACHW4Z_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR8882015.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7933..8097
                     /gene="yhaL"
                     /locus_tag="ACHW4Z_002409"
     CDS             7933..8097
                     /gene="yhaL"
                     /locus_tag="ACHW4Z_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR8882016.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8309..9619)
                     /gene="cyuA"
                     /locus_tag="ACHW4Z_002410"
     CDS             complement(8309..9619)
                     /gene="cyuA"
                     /locus_tag="ACHW4Z_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR8882017.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9647..10978)
                     /locus_tag="ACHW4Z_002411"
     CDS             complement(9647..10978)
                     /locus_tag="ACHW4Z_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8882018.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11253..12617)
                     /gene="tdcG"
                     /locus_tag="ACHW4Z_002412"
     CDS             complement(11253..12617)
                     /gene="tdcG"
                     /locus_tag="ACHW4Z_002412"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8882019.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12689..13078)
                     /locus_tag="ACHW4Z_002413"
     CDS             complement(12689..13078)
                     /locus_tag="ACHW4Z_002413"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR8882020.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13092..15386)
                     /gene="tdcE"
                     /locus_tag="ACHW4Z_002414"
     CDS             complement(13092..15386)
                     /gene="tdcE"
                     /locus_tag="ACHW4Z_002414"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR8882021.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15420..16628)
                     /gene="tdcD"
                     /locus_tag="ACHW4Z_002415"
     CDS             complement(15420..16628)
                     /gene="tdcD"
                     /locus_tag="ACHW4Z_002415"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR8882022.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16654..17985)
                     /gene="tdcC"
                     /locus_tag="ACHW4Z_002416"
     CDS             complement(16654..17985)
                     /gene="tdcC"
                     /locus_tag="ACHW4Z_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR8882023.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18007..18996)
                     /gene="tdcB"
                     /locus_tag="ACHW4Z_002417"
     CDS             complement(18007..18996)
                     /gene="tdcB"
                     /locus_tag="ACHW4Z_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR8882024.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19119..20033)
                     /gene="tdcA"
                     /locus_tag="ACHW4Z_002418"
     CDS             complement(19119..20033)
                     /gene="tdcA"
                     /locus_tag="ACHW4Z_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR8882025.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCR"
     gene            20349..20567
                     /gene="tdcR"
                     /locus_tag="ACHW4Z_002419"
     CDS             20349..20567
                     /gene="tdcR"
                     /locus_tag="ACHW4Z_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR8882026.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            20823..21363
                     /gene="yhaB"
                     /locus_tag="ACHW4Z_002420"
                     /pseudo
     CDS             20823..21363
                     /gene="yhaB"
                     /locus_tag="ACHW4Z_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            21385..22562
                     /locus_tag="ACHW4Z_002421"
                     /pseudo
     CDS             21385..22562
                     /locus_tag="ACHW4Z_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         6190 a         5783 c         5285 g         5683 t
ORIGIN      
        1 attcaatcat caaatttata tatgaccaat ggcataccca tgaataaagt ggtatgataa
       61 cttcgtccgc attccactcc gggagacgca tatggaaatc actgaaccac gccgtttgta
      121 tcaacaactt gctgctgacc tgaaagagcg cattgaacag ggcgtctatc tggtgggtga
      181 taaactgcct gccgaacgct ttattgccga tgaaaagaac gtcagccgaa cggtagttcg
      241 tgaagccatc atcatgctgg aagttgaagg ctatgtggag gtgcgtaaag gttcaggtat
      301 tcatgtggtt tccaaccagc cgcgtcatca gcaggcggct gacaataata tggaattcgc
      361 caattacggt ccgtttgagt tgcttcaggc tcgccagctc atcgaaagta atattgccga
      421 gttcgcggca actcaggtaa cgaaacaaga catcatgaaa ctgatggcca ttcaggaaca
      481 ggcgcgcggc gaacaatgct tccgtgattc cgagtgggat ttacagttcc acattcaggt
      541 agccctggcg acgcagaact ccgccctggc ggccatcgtt gaaaaaatgt ggacccagcg
      601 tagccataac ccgtactgga aaaaactaca cgaacacatt gattcccgta ccgttgataa
      661 ctggtgtgat gaccacgatc aaattctcaa ggcgttaatt cgcaaagatc ctcatgctgc
      721 taagctggca atgtggcagc atctggaaaa caccaaaatc atgttattta acgaaaccag
      781 cgacgatttc gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tagtgcatct
      841 cgatacggcc accagcggca gcaaatgaaa ttcctgtccg acaggcgctg caatgcgcct
      901 gtttgtctcg tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgccac
      961 cctcccctgc aatcagcaaa atcagactcc acggacatgt aattttgata acgaacaacg
     1021 ttgacctttg ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc
     1081 acgttttaac agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa
     1141 aacagcaata acattgcggt agtgcatctt ttaaaaccag cgtggcgtta accgattcac
     1201 caggaataat gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt
     1261 gaaaccctgg ccaatccatc gatgattggc atgttgtatt ttgtcttgtt tgtaattttg
     1321 ttccttgaaa atggcttgct tccggcggcc tttttaccgg gcgacagttt actggtattg
     1381 gtcggcgtgt tgattgcgaa aggcgcgatg ggttacccgc aaacgattct gctgctgacc
     1441 gttgccgcca gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc
     1501 cgcaccgtac aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat
     1561 ctttttcata aacacggttt atcggcgctg ttaattggtc gctttattgc atttgtcaga
     1621 acactgctgc cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc
     1681 aactggatga gcggtctgct gtgggtattg atcctgacaa ctctgggtta catgctcggc
     1741 aaaacgccgg tatttttaaa gtacgaggac cagctgatgt catgcctgat gctgctcccg
     1801 gtggtgctgc tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat
     1861 ggaaatcggg ggtaagggat gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc
     1921 ggcactgttt tacttctggt cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa
     1981 gagtctacgc tggcgattcg tgccgttcat caaggcacaa cgatgccaga cggtttttcg
     2041 atctggcatc accttgacgc tcatggcatt cctttcaaaa gtatcacccc caaaaacgac
     2101 actctgttaa ttacatttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac
     2161 agaacattgc cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag
     2221 tggctgaccc ggttacggga taactctcat cgcttcggat aacttcctgt tatccgaaac
     2281 atatcactca ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac
     2341 actaccgctt cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag
     2401 gttcaagaat gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta
     2461 gttatgccac taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg
     2521 ccgaaaaaca ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc
     2581 gggccaactg ttcagatagc cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga
     2641 aagatgaggt ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg
     2701 atagaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     2761 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     2821 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     2881 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     2941 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     3001 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg
     3061 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     3121 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     3181 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     3241 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     3301 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     3361 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     3421 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     3481 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     3541 actgcttagc cagatccagc aacaacggct ggatatttcc gccagtagtc gtgaatggct
     3601 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     3661 gctggttggc agtagagtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     3721 ctgggccaga cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa
     3781 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccatca
     3841 aattttttga atatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     3901 attctctcca tcgacagcaa cgacgctaat agcgcgccct tgcacaaaaa aacaatcagc
     3961 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta
     4021 gcgcacattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     4081 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gctgacggca
     4141 tttttatttc acagcaactt tgctgaaggc gtcaactcgc tgatgttcat gaaaaacctg
     4201 acaatttctg gcggattcct gctgctggca attaccggtc cgggcgcgta tagcatcggc
     4261 cgcctgctga ataaaaagtg gtaagcacgc gctatactta acgataaaaa acgaggagga
     4321 agctcctcgt ttttgctatt ggaggagaga aaaatgggtc aactgattga cggcgtctgg
     4381 catgacacct ggtacgatac caaatctacc ggcggtaaat ttcaacgttc agcttccgca
     4441 tttcgtaact ggctcactgc cgatggcgca cctggcccca ctggcacagg cggttttatc
     4501 gcagagaaag atcgttatca tctctatgtt tcactcgcct gcccgtgggc gcaccgcacg
     4561 ctgatcatgc gcaaactcaa aggactggaa ccgtttattt ccgtttccgt agtgaacccg
     4621 ctgatgctgg aaaacggctg gacctttgat gacagttttc cgggagcaac cggagatacg
     4681 ctctatcaac atgaatttct gtatcagctt tatctccacg ccgatccaca ctacagcgga
     4741 cgagttactg ttcccgtgct gtgggacaaa aagaaccaca ccatcgtcag caacgaatca
     4801 gcagaaatca tccgcatgtt caataccgcg tttgatgcgc tgggcgcaaa agcgggtgat
     4861 tactacccac cagccctgca aacgaaaatt gacgaactta acggctggat ttatgacacc
     4921 gttaacaacg gcgtgtataa agctggtttt gccaccagcc agcaagccta cgacgaggcg
     4981 gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga ttttaggcca gcatcgttac
     5041 ctgaccggca accagctaac cgaagccgat attcgcctgt ggactacgct ggtgcgtttt
     5101 gatccagtgt atgtgaccca cttcaagtgt gataagcacc gcatcagcga ttacctgaat
     5161 ctgtatggct tcctgcgcga tatctaccag atgccgggga tcgccgaaac tgtcaatttc
     5221 gatcacatcc gtaatcatta cttccgcagc cataagacca tcaaccctac ggggattatt
     5281 tcaattggtc cgtggcagga tctcgatgaa ccgcatggac gagatgttcg cttcggttaa
     5341 aaaaaggcac cttacgaggt gcctctgata attagctaag tgcatgttgt tcatgtcgga
     5401 tgcggcgtga acgccttatc aaacctacaa agcgtttata ttcaatatgt tacaatgacc
     5461 gcgtaggcca gataagcgta gcgcatctgg cgattttgcg cttgccatca gtcttagcgc
     5521 gccttcacgg cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct
     5581 attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg
     5641 gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta
     5701 ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctgc tggataaaat
     5761 gttaggctgg caacgcgctg gtggcgaagg catcctgacg ataatctacg gtattctggt
     5821 gtttttaccg tggtgggcgg ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg
     5881 gtgggcactg ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg
     5941 ccaggcgggt acgccaggcg aaaaccgctt tggaccagat ccaaaactgg aacaagaata
     6001 atgattaagc accttgtaaa aggtgcttca aattgttgac aaagtgcgct ttgtttatgc
     6061 cggatgcggc gtaaacgcct tatccagcct acaaattgcc aaaattcaat atattgcagt
     6121 gactgcgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgtc agcagtcaga
     6181 agcaccttgt gaaaggtgct taatagatta ttttccagcg aacaactttg gaatttcccg
     6241 cagacaccag gatttcgctt cccccatact gtcacgacgc caggccataa taatatcgat
     6301 ctcgctggtc gattccgggc tgacgacacg caaccgccct tccgcaatat ctttttcgac
     6361 catcggatac ggcatcgtcg ccacgccaag ccccgccagt aatgcctgac gtttatcttc
     6421 aatcgtgctc accgttaagc gcggctgttt gtccagcagc tgtacggtca acaccgggcg
     6481 ctcacgagcg gtatccgcta ccgcaattcc acgatatttc acacgcgtca cttcagataa
     6541 cggttccggc tcctgatgaa tcgggtgatc aggcgcggca acgtagacgt tcattaacgt
     6601 atagagcttg cgcgagttga tctccgacga ggaacgaaaa tgcatatccg gcgcgataac
     6661 gatatccgcc cgcccctgct ccagccgttc ccacgccccc gccagcactt ctgtgatgat
     6721 tgccagttgg gtattggctt ttgccgccag tttgtcgatt aacgggaaaa aggcaggtgt
     6781 cggtaccagc gcttcggtca caatggtgag atgcgtttcc caaccgcgcg cgagagcttc
     6841 cgcatcagta gtcagtttat ctgcggcttc cagcaaaacg cgccctcgct ccagcaacat
     6901 cctcccgaca ttggtgaatt tggtgcgatg gcccgagcgg tcaaacagca ccacatccag
     6961 ctcttcttcc agtttttgca tggtgtagct aagtgcggaa ggcacgcgtc ccagctcatc
     7021 cgctgccgcc gcaaaactgc cccggcgatc gatcgcatcc ataacccgta gtgcttccag
     7081 cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg aaatttgaac ataccgggca
     7141 gaatatctgg ctaacaatgt agcgtccagc cccttaccat aaaaggaagt aaagagaggt
     7201 caagaattat gattactacc cgaactgcca ggcagtgtgg acaagcagac tacggatggt
     7261 tgcaggcccg gtatactttt tcctttggac actacttcga cccgaaattg ttaggctatg
     7321 cctccctgcg tgtgcttaac caggaagtgc tggccccagg tgccgccttt cagccgcgaa
     7381 cctatcccaa agtcgatatt ttaaatgtga ttctggatgg agaagcagag tatcgcgaca
     7441 gcgaaggcaa tcatgttcag gccagcgccg gagaggcgtt gctgctctct acccagccgg
     7501 gtgtcagcta tagcgaacac aatctcagca aagacaaacc gttaacgcga atgcagcttt
     7561 ggctggatac ctgcccgcag cgagagaatc cgctgattca aaagctggcg cttaatatgg
     7621 acaagcagca attaatcgcc tcgccagagg gtgcgatggg aagcctgcaa ttacgccaac
     7681 aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag tgcgaatttc cagttgcatg
     7741 ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca tgcgcttacg catcatgaag
     7801 agaaagcggc gctaacctgc ggtgatgggg cgtttattcg tgacgaggct aacattacgc
     7861 tggttgccga ttccccactg cgcgctttgc tgatagattt gcctgtctag ttgtttttac
     7921 agggagatga tgatgagtaa aaaatcgacc aaaaagcgcc agccagcgaa gcccgtggtg
     7981 gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg aagagatgtt gagcgagctg
     8041 gaagctatcg tcgcggatgc tgaaacgcgt ttagccgagg atgaagctac cgcgtaaaga
     8101 caggttgccg gatgcggcgt aaacgcctta tccggcctac aaaacacaac aacatcaaca
     8161 aattaaacac cccgtaggcc ggataagatg cgccagcatc gcatccggca tcaggtacaa
     8221 aacacaacaa catcaacaag ttgagcaccc cgtaggccgg ataaggtgcg ccagcatcgc
     8281 atccggcaca atactcaaaa cctggcgttt atctggcctt gctcgccata atctcgataa
     8341 tctgccgatc cgtttgctgc atcgaatggc ttgctaacgc acacaggttg gcaatcgact
     8401 gctcaacatc atgcgccacg atcccttcat tgccggtcac ggcggtatca tccagcgcca
     8461 ttaacaccgc tttccacgca gccgaagcac tggtcgaaac cttcatcgcg cagctgttcg
     8521 acgcaccatc gcaaatcatg ccgctgacat cgccgatcat actgctgatc gccatcgaga
     8581 tagtttcata acgcccatcc accagccatg ccatcccggc ggcggccccc attgctgcgg
     8641 tcgttgcggc acaaagtgca gacaaacgcg gtaactggtt atggatgtaa attgcgctca
     8701 aatgagaaag catcagcgca cgcgccagtc gttcatcatc cgctccgaag tgttctgcta
     8761 caaccaccac aggcattatt gcagtgatcc cctggttacc cgagccggag ttactcatag
     8821 ccggaagcgt agcgccgccc atacgcgcat cggatgccgc gctggtacga atcacaatgg
     8881 atgaagagag atctttcgcc agcaaaccgc gctcgcactg tttttccagc gtcgcgccaa
     8941 tatgcagccc ccacttaccg ctcaaacctt cctgcgataa cgcacaattt agcttcgcgg
     9001 aatcgagaat aaagcggatc gccgcaaatg ggacttcatt gacgaacttc aggatctcag
     9061 ccagcgtcgt tctggaaagc accgtcagcg gcgactcttg ctcgccctct gccacacacg
     9121 cctggtgggt aaacaccaca ccattgtgcg tctcaatatg cacaatgttg gtatgcccgc
     9181 cgacgatggt gacacacgcc cacttctcac cgttccagac tttggcgcgt gagaagagga
     9241 tttcattgca aggttcctgg atcttaacag agactttccc cgccgccagc agtgctttgg
     9301 catcggtaat tgcctgcgca gttgcgtctt tcagcacttc cagcccggcg ttggcatttc
     9361 cacctaacgc ccccagcgcc gccgcaatcg gcagccccac cattcccgtg ccgggaacgg
     9421 tgacgcccag accgttcttc atcagatttg gcgaaaccca ggcttctaca cgttcaaccg
     9481 gaccttccag ttctgccgca gcaaccgccg ccgccagcgc cagtgaaatc ggttcagtac
     9541 atcccagcgc cggttttact tcctcctgaa cggcgaggat gtaacgctgc cataacggat
     9601 ttaaagtcga atcaaacata ttaaaaacct taaaatttca ggtaaatcaa gaaaatgcca
     9661 ggaacagaga aacacaaagc aacaaaccag tgacgataat caggtacaga gacatccctt
     9721 tgtatttgtg caatgccggt actttgtaaa ccagccacgc cgggatcagg caccctacca
     9781 tgccgaaaat cgggctacag atagaggtga agctcaacac cggtgcgttc agtacgatgg
     9841 cgctccaggc cagcaaaatg gcgaaaatca tgatgccgcg ctgaacaaga ttttcgttaa
     9901 tcttctcggc aggcatcttg cgacgcagga tgttcattac gatcccttgc gttgcttcgc
     9961 gaaagcctaa atagacgcca aagaatgcag tcatgacagc aaagatattg agaatgacgc
    10021 tgaccacttt cacccacgct gcgccgtcac cgctaataaa ctgtgcggca atcgccagcg
    10081 cggaaatatt ctgctcgtag gcttttaccg cttcgtcatg tcccatcgcc agcgtgaacg
    10141 acacggcgta gaaaaagacg gtgacaaaca aaatgccaaa cgcgatattc attgcccgca
    10201 atgctttatg ccgcgccact tcaattgatt tttcccgcga gcgataagag atcaccatcg
    10261 ggcttaacgt ctggataaac agaatcgacg tcagggtaaa cggcagcgta ataatggcgt
    10321 ttttcaccag cagccccagc ggcggtagcg aaccgacgtt atacagatgc cacattccca
    10381 ccatcgacac gcccagcgca gcgactacca gcagcttggt cagcaccatg tcggtcgaaa
    10441 ttttgaataa caatttctcg ccgcgtgaag agatcgccac cagaatgcaa atcagtacca
    10501 gaccataaaa gggactgtct gacagcaacc cttccgtcac gccgaaggta tgcagatagg
    10561 aagcactatc gttggtgatg gcggtggaat agacgaacat ccaaatcacc agcattacga
    10621 aatagagcgc acctaacagg atgccccagt ttttacctaa ataaccgcta atgacactcg
    10681 ggtaatcttt acattctggt gattctgcca gcgtattaat aaacaaccgc tgaaacagat
    10741 acattgccgg gtaaccaatc accgatgaga gcaaaaatac ccacaatccc atcaaaccga
    10801 cctgcaccgg gagaaaaaca atccccgcgc caatcgccat cccgatactc ataatcaccc
    10861 agccggtgtc agtactgtcg aatttgatcg cttctcgcca ctcgctctca ctcattccag
    10921 cacgacccgc cggggtcgaa gcgtctgcaa tgacgccttt attcgatgca atttccataa
    10981 tttctcgctc aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat
    11041 gcggtctacg tttttgcagg cgagttatta gaataaagaa atgatacgcg cgaggtcaga
    11101 gaaaatcttc acaatcaaga ctttaaaata acggagatgg ataagaattt tctactaaat
    11161 taatcacaag agaatattca ttctctattt gcggcagatc acaaaaaaaa ggtgcacatt
    11221 tgtgcaccca aggatgaaag ctgacagcaa tgtcagccgc agaccacttt aatggccagt
    11281 cctccgcgtg atgtttcgcg gtatttatcg ttcatatctt tgccggtttc atacatcgtc
    11341 tcgatcactt tatcgagtga aacacgcggt gccgaggtgc ggcgcatcgc catccgcgcg
    11401 gcgtttactg ctttcacggc attaatggca ttacgttcaa tgcacgggat ttgtacctgt
    11461 ccggcaaccg gatcgcaggt cagcccaagg ttatgctcca tcgcgatttc cgccgcattg
    11521 catacctgcg ccggactgcc gcccagtagt tcagttaacc ctgccgctgc cattgaacag
    11581 gccacgccaa tctccccctg acagccgacc tccgcgccag agatggaggc gttcatttta
    11641 tacagcgcgc caatagcccc cgctgccaga aaatagcggg caattgaccg ctcgtttacc
    11701 ggacgacgga acttatcgta ataagccagc actgccggaa taatgccgca cgcaccgtta
    11761 gtcggtgccg ttaccacgcg cccgccagct gcgttttctt cactaaccgc cagcgcgtac
    11821 atgttgatcc agtcgatgac attcatcgga tcgttagaga tgttatcgct agaaaccagc
    11881 tgacgacgca gcgctacggc acgacacggc acattgagcg gaccaggcag cacgccttca
    11941 gtgttcatcc cgcgttcaat accgtcatgc atcacttgcc agatacgggc aaaccctgcg
    12001 tcaatttccg ctttgctgcg cagcgccagc tcgttgtgca tcatcaggcc agatatagac
    12061 aggccgttgt aatcacacat tttcagcagt tcacctgctg agtggaaatc gtaaggtacg
    12121 gacgtttcga catcgtgcga caggccgaag tgttcttctt cgacaataaa cccgccgccg
    12181 acagagtaat aggttttact taatagcgct tcctgtccct tccaggcagt gatccgcatt
    12241 ccgttctcat ggcgaggcaa catttcggga tggaagataa tgttctttgc tacaggaaaa
    12301 tcaacaatat gcgcaccaga tgccactggc agccgcccgc tgcgcgttac taactctata
    12361 aatgcaggga tctcatcaat gacaacatcc tgcggactgt ttcctgccag tcccatgata
    12421 atggcgacat ccgtggcatg gcctttgccc gtcagtgaca acgacccgta cagatcgacc
    12481 acaatatggc tcgtcgcggt taataagccg ctactttcca gccgatcaat aaaacttttt
    12541 ccggcattca ttggccccac ggtatgcgaa ctggagggac caatcccaat tttgaaaata
    12601 tcgaatgcac taatcatatc cacaccctcg gattgccgtt cagtgaagtg gagcggaacg
    12661 accttgcgac cgtcccgctc acgaggcttt acgcactacg tactgcgata gcttcaattt
    12721 ccagcttcac atctttcggc aaacgcgcaa cctgcacaca gctccgggtc gggtaggtcg
    12781 cctgatgctc atcgaagaac tgcttataga cttcattgat ggtggcaaaa tcattcagat
    12841 cggtgatgaa cacggtcatc ttgatgatat cgcccacgct cagcccggca gcaaccacga
    12901 tcgctttgac gttttcgagg cttaaacgcg cctgatcttg cacatcagcc gggatctcac
    12961 cggtctgcgg gcaaaccgga atttgcccgg aggtgaagac catgctgcct aaatcaacgc
    13021 cctgaacata agggccgatt gcgcctgggg cacgttgcgt ttcgataatc tttttcatac
    13081 atcctccggc gtcagagcgc ctgggtaaaa gtacgtgaaa taacatcctg ttgctgttca
    13141 cgggtcagtg cgttgaagcg cacggcgtag ccagagacac ggattgtcag gttaggatat
    13201 ttttccgggt gctcgatggc atccagcagc atttcccgat tcattacgtt gacgttgagg
    13261 tgttgaccgc cttcgacatc cgcttcgtgg tggaaatacc catccagcag gccgacaagg
    13321 ttggttttac gtactggatc ttctttgccc agcgccgcag gaacgattga gaaggtgtac
    13381 gagatcccat ctttggcgta ggtgaacggc agtttcgcca ccgacgtcaa tgaggccacg
    13441 gcacctttgc ggtcacgacc atgcatcggg ttagcgcccg gcgcgaacgg tgttccggcg
    13501 cgacgaccgt ccggcgtatt accggttttc tggccgtaca ccacgttaga agtgatagtc
    13561 agaatcgact gggtagggac ggcgttgcga taggttggca gcgctttaat tttcttcata
    13621 aagcgttcaa ccaggtcgca ggcaatgctg tctacgcgct cgtcgttgtt gccgtactgc
    13681 ggatattcac cgtcgatttc aaagtccacc gccaggccgt tttcgtcacg gattggtttc
    13741 acgcgggcat atttgatggc agacagggag tccgtcgcca ccgacaggcc cgcgatgccg
    13801 catgccatag tgcgatagac atcacgatcg tgcagcgcca tcagcgaagc ttcgtagctg
    13861 tacttgtcgt gcatgtagtg aatgatattc agcgcgctga tgtactgcac cgccagccag
    13921 tccatgaagt gatcgaggct gtccatcact ttgtcgtaat ccagcacgtc gtccatcagc
    13981 ggtgctgttt tcggcccgac ctgaatcttc agcttctcgt ccaccccgcc gttaattgcg
    14041 tagagcagcg ttttcgccag gttagcgcgt gcaccaaaga actgcatttg cttaccaatc
    14101 accattgggc tgacgcagca ggcaatcgcg taatcgtcgc tgttgaagtc agtacgcatc
    14161 agatcgtcat tttcatactg caaggaagag gtgacgatcg acacctgcgc ggcatatttt
    14221 ttgaaggcaa tcggtaattc ttccgaccaa agaatggtca ggttaggttc cggtgccggc
    14281 cccatagtgt gcagggtgtg caaatagcgg aaggagtttt tggtcaccag cgtacgaccg
    14341 tccagcccca tcccgccgat cacttccgtc gcccagattg ggtcgccgga gaacagcgaa
    14401 tcaaattccg gtgtacgcag gaagcgtacc atacggatct tcatgatgaa gtgatcgatc
    14461 agttcctgtg cctgctgctc attgaggacg ccagctttaa agtcgcgctc aatgtagata
    14521 tccaggaacg atgccgtgcg ccccagcgac atcgcgccgc cattttgcga tttcactgcc
    14581 gccagataag cgaagtagag ccactgcacc gcttcctgtg cattctgcgc cgggcgagag
    14641 atatcaaagc catatttcgc cgccatttcc tgaatctgca acagcgcatg acgatgctct
    14701 gccagctcct cacgcagacg gatggtggct tccagatcct cgcctttttc cagacgagac
    14761 tggagatcgg caaattgcag ttcgcgttca cgcaccagat aactgatgcc atacagcgct
    14821 acgcggcgat agtcaccgat aatgcgccca cgaccatagc catctggtaa accggtcagc
    14881 acgccagatt tacggcagcg cagcatatcc ggtgagtaaa catcaaatac gccctggtta
    14941 tgggttttac gcagatcggt aaacagatat tcaaattcac tgtccatttc acggccatag
    15001 gcgtggaatg aacttttaat catattaatg ccaccgaacg ggtgtagcgc acgtttcaac
    15061 ggcgcatccg tttgcaggcc aacaattttt tccagcggct ggttaatata tcccgcatca
    15121 tgagcggtaa ttgtggtggc aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt
    15181 tcgatacgga tgccttccat taccttttcc cacaattccg tggtggcagg cgtcgcttcg
    15241 gcgagaaaag attcatcgcc ttcatacggt gtatagttat gttgaataaa atcgcggaca
    15301 ttaatttcgc ttttccagtc cgtaccttta aagccaagcc atgcgtcagc gtacagctta
    15361 tcgctggtat caatatctac cttcatgaaa aataatctct ctacaatact tcaactaaat
    15421 tatgcaaatt ctgcgggcgc gttaacttta cctaaatgaa tggcatccaa agcaatcatt
    15481 ttttcttcgt tagtcggaat aacgacgcaa atgacatgcg cattttcact ggaaacaatt
    15541 cgctcgccaa aagagttaga acgattattc atttctgtat caatctctac gcctaataca
    15601 gccaaatgtt ccatgaccag acgacgaatt aaacttgaat tctctcctat tccgccggtg
    15661 aatataattc catccagccg acgtaatgaa gctgcatgtc cggcaatatg acgggcaatt
    15721 cggtgaacaa aggttttaat tgccagttgc gcgcgttcgt gaccttcatg ccaggctttt
    15781 tccagaacac gtaaatccga agaaagaccg gaaataccta ataatcccga ctctttattc
    15841 actacgcgtt ccaggtcacc caggctctgg ttggtttggc tggcgaccca ggacatcgca
    15901 ccaaagtcga catcgccact gcgggtaccc atcatcaagc cttccagcgg cgtcattccc
    15961 attgaggtgt caacactctg accgttgcga actgcgcaga ttgacgcgcc attgccaaga
    16021 tgcgccacaa ccaggccgga gtcatcttcc gccagattca gcagcgaatg tgcgcgctgg
    16081 gaaacatagc ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa ctcttcataa
    16141 tatttccacg gcaggccgta taaataagct tccggagcca tcgtctggtg gaaactggta
    16201 tcaaataccg ccacctgagt tacgcccgga aataattgct gcgccgattc aataccactt
    16261 aaatttgcgt aattatgcag gggtgccagt ggagaaacgc gacggatatt atcaatgact
    16321 tcatcggtaa taatggcgga ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca
    16381 attaaggcca cgctgtcatt taaattccgt ttttccagtt caaatgcaat tgccttcaat
    16441 gcaccttcgt agctgtggtg agccagcggt gctggctctc ccccatttac ggataagaat
    16501 gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca atcgctggca
    16561 tcgagtacgg aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat
    16621 tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata cgatgttcag
    16681 gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc
    16741 cagagacggc gctttacgga tagcatacat cggcaacagg cacagcaggg atgcgataat
    16801 cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca
    16861 ggtggagccc atgatgaaga tcatgctgag agtattcagt ttacccagcg acactttggt
    16921 tttgtcacct ttataaccga acttcagaat cagaccattc aagccttcca gcgtccccag
    16981 atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc
    17041 cagtgtaatc gcgaacgttg ttttcgtacc ggtcatggac gcaaagtggt tagccagata
    17101 agaaagcact ggaatattct gcgctttggc ttccgccatg ttggccggag acagagtaaa
    17161 caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat
    17221 gatttgggaa catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg
    17281 tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga
    17341 aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag
    17401 gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacagag aaatcagcac
    17461 caggctggcg ataaacggcc ataccaggta gctcatcact ttaaccatca gatccttacc
    17521 aaaccagatg acgaaagcca tcagcagcaa caggaacagc gccacaaagc cgcgattcag
    17581 cggtgcaaat ccgagctggt tttcccagaa cgtcataaag gtattggtaa tagtaacgcc
    17641 ataaatccac agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc
    17701 agttttacca aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt tagagccaga
    17761 aagacacaga cgcgccagcg cccggtggca ataaaatgcg atggggtatg ccaataccaa
    17821 catcagaaga atcgggatca gtccgccaaa acctgcgcgg atagggaaga acagcacccc
    17881 ggcgccgatt gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt tacgccagga
    17941 cgattgtttt gtctggctgg atacaatgct atctgaagta ctcatatcct atcctcaacg
    18001 aattaattaa gcgtcaacga aaccggtgat ttgagagacg cgagaaagat cgatattgcc
    18061 gccggaaata atactgacgg tttttctatt ttgaatatat tggtctaatt taccgcttaa
    18121 taatgcagca catgccagag cgcctgcgcc ttcggtgacg actttattgc gctgaattaa
    18181 ggcaatcata ctgtttctga tttcgtcttc gctgaccagc acgatgtcat cgactaattc
    18241 acgaacgatt tcgtaagtta aattacccgg gcgggagaca tcacaaccat ccgccagggt
    18301 gccggtagtt cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac
    18361 gttttctgac tgtacgccaa taacacgaat ggtcgggtta atagatttaa ttgccaccgc
    18421 aataccagca attaaaccac caccaccaat tggcacaatc acgttatcga catcatagag
    18481 atcttccata atttccagac caatcgttcc ctggccagca atcactttcg gatcatcgta
    18541 aggtgggata aaaatacgac cttccatttc gacaatttcg ctcactttag cgatagtgtc
    18601 gttgaagtta tcaccatgca gaacgacttc tgcggagtag tcgcacgttg ccgctacttt
    18661 ggattttggc gcaccttttg gcatcaccac tttaccgtcg atacccagca tcgcgcagga
    18721 gagcgaaacc ccttgcgcat ggttgcccgc agaacaggcc accacgcctt tgcgtttttc
    18781 cgcatcggtc agtgaactta atttattaaa tgcgccacga attttaaatg aacccgtacg
    18841 ctgcatattt tcaaacttca ggaatatttc acctttgcaa cgttcactaa aatagttgga
    18901 gcgaggcatg cctgttttat aaattcgccc agccagtcgt tgtttcgctt caataatatc
    18961 atcaatagca accggcagat cgtatgtaat atgcattata aaacctcttc gcctgaatta
    19021 aatatgtagg taaccgtaac cgacacctgc aaaacagaca ggtggattat ttatgtttaa
    19081 ataaaaacaa acaactaacc aacttctatt aattgccttc atctacaccc attataagat
    19141 gaatactctt tggctaattc caccaaaacc gatgctgctt ttttaatacg ataatttttc
    19201 gaccataccg cggcatattg tgccacaggt aatgtttctt caaccggaat agtaataaat
    19261 tgattagaac caaaaggtga cgtcatatca caaggaatta cagttaagaa atcagcattg
    19321 agaacaagat tataaattgt cacgactgag tcggttttaa cgatgttttc aatactgatg
    19381 ccatttcttt gtaacgtagt aagcagttcg ctgtagtacc ccatattagt ttgtggcaac
    19441 acccactgtt cgttcttcaa cgactccagc gtggtggtgc cggtgcatgt tcgggactta
    19501 ctggctacca gcacaaactc ggactcgaac agcggctcaa catgtaaatc ctgaagcttc
    19561 atttctgcac ttaacgtacc aatcgcaaaa tccagtcgac cgtcgcggat tgccggtaag
    19621 aacgaagaca gttgcgcttc atacatagaa acctgcgctt tcgggaacac ctctttgaac
    19681 ttgttgatca tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact
    19741 tccaccaccg cttcagaaga cataccgctt atctcattaa ccatattttt catttcacgg
    19801 gtaatggatt cggaacggga gagtaacaat tgaccggcag gtgttaatgt tacaccggta
    19861 tttttccgca ccactaattc cacaccaaaa taatcttcaa tatcgttaat gattttactg
    19921 acggccggtt gagttaaccc taattctttt gcagccgagc cgatagagcc acttctaatg
    19981 acttcctgaa agactaccag gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc
    20041 tacgttaatt acctcattga cggcatgaag tgtatcaaaa tgaaataaac aggatatgtg
    20101 cgaccactca caaattaact ttcattactt tccaaggtat cgttattaca attaaacatc
    20161 tttaaatatc aacaagttaa agcataaaaa tcagcataaa ccctgatttt tatcaaaaaa
    20221 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
    20281 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt
    20341 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
    20401 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
    20461 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
    20521 tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct
    20581 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctggtcgccg
    20641 cgataaggtc agacgaagtc aacaaggggg attttcactg tgctttacat cagcgtagga
    20701 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt
    20761 aagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
    20821 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag
    20881 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttgcagaaa
    20941 ttgtctccac caatgacgtt aataaaattt acagtataag taatgaactc cggagagtat
    21001 tatctgcaat tactgcattg aatttctatc aaggcgatgt tccttctgtc atgattccga
    21061 atccaaccgg aaaatatgag tccattcatc atagatattt ctacaggaga acatgatgat
    21121 tatatcatac aaacattaga tgtaggcact tttgcacctt ttggtgaaca atgtacttgc
    21181 tcagccgtca ataaaaaaga gctggaatgt attaaggaga cgatttctaa gtattgcgca
    21241 aagttcaccc aaaaagaagc catcttaaca ccccctaggc actttaataa aacctgcatt
    21301 acttcagatt gttggcaaat tctctttttt tcacccgatc attttaataa tgatttttat
    21361 tgattattta taaaggataa aataatgttc cctgtttcat caattggtaa tgatattagt
    21421 agtgatttag tccgtcgaaa aatgaatgac cttcctgaaa gccccatagg aaataacctc
    21481 gaagcactag caccaggtat agaaaaacta aaacagacct ctattcaaat ggtcacttta
    21541 cttaacgcgt tacaaccaag tggaaaatgc attatcactg gtgattttca aaaagaatta
    21601 gcgtacttac aaaatgtaat tctttataat gactcgtctc ttcgtctgga ttttttaggt
    21661 tataacaccc aaattattca acgatcggac aatacctgcg aacttaccat taatgaaccg
    21721 ttaaaaaact aggaaatatc tacaggcaat atcaatgtta attgcccatt aaaagatatt
    21781 tacaatgaaa tcaggaggtt gaacgtaatc tttagttgtg ggactggaga tatagttgat
    21841 ctatcctctc tggacttacg taatgtcgat ttagattatt atgatttcac agataaacat
    21901 atggctaata ctattctaaa tcattttaaa ttggattcaa caaattttac taatgccaac
    21961 atgtttcagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
    22021 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
    22081 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
    22141 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
    22201 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
    22261 ttaccagaga tgaaagaatt ctcatcaaaa aaaacatatg attacatcga tacaatcatt
    22321 gaagattatg aaaatatcaa agaaaatgct tatctggtcg tcccccaaat taattatact
    22381 atggatttaa acatagaaga ctctagctca gaagagttaa taccctcgag aaagacgaaa
    22441 attctccgga caatggcttt gaggtcgggg aatataacac atatgaagca tataactcag
    22501 agaatcaata ttttaccaga gaggactata cgtataatta cgacctttta aatgcaatat
    22561 agacttaata acttcttaca aagtctatta ttaataccgt agatattcat cttaccctgt
    22621 gcaccaaaag caaaaactta tcgacgcggg tttgaaactg atggcaaccg caaaaatgcc
    22681 tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt
    22741 tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa taaagcgcac tcaacaatct
    22801 gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa ctgaccgata agccgctttc
    22861 ttttgggtat agtgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc
    22921 gccgaagcgg gtttttacgt a
//
LOCUS       DAZSNG010000059        22536 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZSNG010000059 DAZSNG010000000
VERSION     DAZSNG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22536
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.060"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="ACHW4Z_002422"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="ACHW4Z_002422"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR8882027.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="ACHW4Z_002423"
     CDS             691..1317
                     /locus_tag="ACHW4Z_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR8882028.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3417
                     /locus_tag="ACHW4Z_002424"
                     /pseudo
     CDS             1338..3417
                     /locus_tag="ACHW4Z_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3430..4068
                     /locus_tag="ACHW4Z_002425"
     CDS             3430..4068
                     /locus_tag="ACHW4Z_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR8882029.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4132..4800
                     /locus_tag="ACHW4Z_002426"
     CDS             4132..4800
                     /locus_tag="ACHW4Z_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8882030.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4797..5582
                     /gene="phsC"
                     /locus_tag="ACHW4Z_002427"
     CDS             4797..5582
                     /gene="phsC"
                     /locus_tag="ACHW4Z_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR8882031.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5586..6398
                     /gene="ydhT"
                     /locus_tag="ACHW4Z_002428"
     CDS             5586..6398
                     /gene="ydhT"
                     /locus_tag="ACHW4Z_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR8882032.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6410..7832)
                     /locus_tag="ACHW4Z_002429"
                     /pseudo
     CDS             complement(6410..7832)
                     /locus_tag="ACHW4Z_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7958..8263)
                     /locus_tag="ACHW4Z_002430"
     CDS             complement(7958..8263)
                     /locus_tag="ACHW4Z_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR8882033.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8371..8447)
                     /locus_tag="ACHW4Z_002431"
     tRNA            complement(8371..8447)
                     /locus_tag="ACHW4Z_002431"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8411..8413),aa:Val,seq:gac)
     gene            complement(8452..8528)
                     /locus_tag="ACHW4Z_002432"
     tRNA            complement(8452..8528)
                     /locus_tag="ACHW4Z_002432"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8492..8494),aa:Val,seq:gac)
     gene            8836..10092
                     /locus_tag="ACHW4Z_002433"
     CDS             8836..10092
                     /locus_tag="ACHW4Z_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR8882034.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTAKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10133..11506)
                     /gene="mdtK"
                     /locus_tag="ACHW4Z_002434"
     CDS             complement(10133..11506)
                     /gene="mdtK"
                     /locus_tag="ACHW4Z_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR8882035.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11721..12362
                     /gene="ribE"
                     /locus_tag="ACHW4Z_002435"
     CDS             11721..12362
                     /gene="ribE"
                     /locus_tag="ACHW4Z_002435"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR8882036.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12402..13550)
                     /gene="cfa"
                     /locus_tag="ACHW4Z_002436"
     CDS             complement(12402..13550)
                     /gene="cfa"
                     /locus_tag="ACHW4Z_002436"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR8882037.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13841..15052)
                     /gene="punC"
                     /locus_tag="ACHW4Z_002437"
     CDS             complement(13841..15052)
                     /gene="punC"
                     /locus_tag="ACHW4Z_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR8882038.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15165..16097
                     /gene="punR"
                     /locus_tag="ACHW4Z_002438"
     CDS             15165..16097
                     /gene="punR"
                     /locus_tag="ACHW4Z_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR8882039.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16094..17119)
                     /gene="purR"
                     /locus_tag="ACHW4Z_002439"
     CDS             complement(16094..17119)
                     /gene="purR"
                     /locus_tag="ACHW4Z_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR8882040.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17418..17507
                     /gene="cydH"
                     /locus_tag="ACHW4Z_002440"
                     /gene_synonym="ynhF"
     CDS             17418..17507
                     /gene="cydH"
                     /locus_tag="ACHW4Z_002440"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR8882041.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17673..18842
                     /locus_tag="ACHW4Z_002441"
     CDS             17673..18842
                     /locus_tag="ACHW4Z_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882042.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18988..19569)
                     /gene="sodB"
                     /locus_tag="ACHW4Z_002442"
     CDS             complement(18988..19569)
                     /gene="sodB"
                     /locus_tag="ACHW4Z_002442"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR8882043.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19697..20524)
                     /gene="mepH"
                     /locus_tag="ACHW4Z_002443"
     CDS             complement(19697..20524)
                     /gene="mepH"
                     /locus_tag="ACHW4Z_002443"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR8882044.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20859..21206
                     /gene="grxD"
                     /locus_tag="ACHW4Z_002444"
     CDS             20859..21206
                     /gene="grxD"
                     /locus_tag="ACHW4Z_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR8882045.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21320..21730)
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_002445"
                     /pseudo
     CDS             complement(21320..21730)
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            21753..>22536
                     /locus_tag="ACHW4Z_002446"
     CDS             21753..>22536
                     /locus_tag="ACHW4Z_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR8882046.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNH"
BASE COUNT         5855 a         5247 c         5826 g         5608 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgcagatcc caagtcacgc tggacagcac gtaaagggct gttttggggc
     2101 gcggctgaag gtggtccgat tgaaacgggt gaaattccgc caggcaatca gaatacggtc
     2161 ggctttcgta cctataaatc cgtttttgac ttaggaccgg cggcagagaa atacacagta
     2221 aaaatgagcg gctgccactc ttgcccgatc cgttgtatga cccaaatgaa tattcctcgg
     2281 gtgaaagagt ttggcgtgcc cagcacaggt ggtaacactt gtgtagcaaa ctttgtccat
     2341 accaccatct tcccgaacgg gccgaaagat tttgaagata aagacgatgg tcgtgtgatt
     2401 ggtaacctgg ttggtctgaa tttgttcgat gactacggcc tatggtgtaa ctacgggcag
     2461 ttgcatcgcg actttactta ttgttacagc aaaggtgtgt tcaagcgtgt tctgccagct
     2521 gaagagtatg cagaaattcg ctgggatcaa ctggaagcgg gtgacgttaa cttcattaaa
     2581 gatttttact accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca
     2641 tatgccatcg cagaacgctg gaatttgggt aaagagtact ggggctacgc gaaaaataaa
     2701 ctctggtcgc cgtttggcta tccggttcac catgccaatg aagcgtcagc gcaggtcggt
     2761 tccattgtta agtgtatgtt caaccgtgac tgcatgacgc atacccatat caactttatt
     2821 ggttccggct tgccattgaa actgcaacgt gaagtggcga aagaactttt tggttctgaa
     2881 gatgcttacg atgaaaccaa aaactacacg ccaatcaatg acgcaaaaat taaatatgca
     2941 aagtggtcgc tgttgcgggt ctgtttgcat aacgccgtca ctctgtgcaa ctgggtctgg
     3001 ccaatgaccg tttcgccgct gaaaagccgt aattaccggg gcgatctggc gcttgaagcc
     3061 aaattcttca aagcgatcac cggcgaagag atgactcagg aaaaattaga tttagctgca
     3121 gagcgtatct ttacgttgca tcgtgcctac acggtaaaac cgatgcaaac caaagatatg
     3181 cgtaatgaac acgatcttat ctgttcctgg gttttcgaca aggatccgca gatcccggtc
     3241 tttactgaag gtactgacaa aatggatcgt gacgatatgc atgcttcgct cacgatgttc
     3301 tacaaagaaa tgggctggga cccacagctt ggttgtccaa cccgcgaaac attgcagcgt
     3361 ctggggctgg aagatattgc cgccgatctg gcagcacaca atctactgcc tgcgtaagga
     3421 atgggtaaga tgaaccatca ggatgaattg ccgttagcga aagtcagcga agttgacgag
     3481 gcgaagcggc aatggttgca ggggatgcgt caccccgttg atacggtaac cgaaccagaa
     3541 cctgctgaaa tcctggctga gttcattcgc cagcattctg cggctgggca gctggtggcg
     3601 cgcaccgtgt ttctctcgcc accctattcg gttgcagaag aggaactgtc agttttgctg
     3661 gaaagcatta agcagaacgg agactacgcg gatatcgctt gtatgacggg aagtcaggat
     3721 gattactatt actcaacgca ggccatgagt gaaaactacg cggcgatgtc tttgcaagtt
     3781 gtggagcagg acatttgtcg cgccattgca cacgccgttc gttttgagtg ccagacgtat
     3841 cctcgtcctt acaaagtcgc catgctgatg caagcgccat attacttcca ggaagcgcag
     3901 attgaagccg cgatcgccgc gatggatgtt gcacctgaat atgccgatat acggcaggtg
     3961 gagtcatcga cggcggtgtt gtacttgttt agcgagcgat ttatgaccta tggcaaggct
     4021 tatggcttat gcgaatggtt cgaggtagag cagtttcaga acccctgaaa tttcagggag
     4081 atgtcatgga tagggtggac agttgcagcc actgcattag gggataatca gatgtccttc
     4141 actcgacgca aatttgttct ggggatggga acagtaatct tttttactgg ttctgcgtct
     4201 tctctgttag cgaacacgag gcaagaaaag gaagttcggt atgccatgat tcatgacgag
     4261 tcacggtgca atggctgtaa tatttgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc
     4321 gctcagggaa gccgtttatc gatagcacac attccagtca ccgataacga caacgaaacg
     4381 cagtatcact tttttcgcca gtcatgtcag cactgcgaag atgctccgtg catcgacgta
     4441 tgcccgacgg gggcatcgtg gcgtgatgag caggggatcg tgcgggtaga aaaatcgcag
     4501 tgcatcggct gcagttattg cattggcgct tgtccgtacc tggttcgcta ccttaaccct
     4561 gtcactaaag tggcagataa atgtgatttc tgcgctgagt cccgactggc aaaaggcttt
     4621 ccgcccattt gcgtcagcgc ctgtccggaa cacgcattga tctttggtcg cgaagatagc
     4681 ccagaaattc aggcgtggtt gcaggataat aaatactatc aatatcagct acctggtgcc
     4741 ggaaaaccgc atctgtatcg ccggttcggt caacatttga ttaaaaagga aaatgtatga
     4801 acccgtcgca acatgctgaa cagtttcaga gccagttagc gaactatgtg ccacagttca
     4861 ctcccgaatt ttggccggtg tggttgatca ttgccggagt attgctggtt gggatgtggt
     4921 tggtgctggg gctgcacgcc ttgcttcgtg ctcgtggcgt gaagaaatca gccaccgatc
     4981 atggtgagaa ggtttatctt tacagcaaag cggtcagatt atggcactgg tcgaatgcgt
     5041 tactctttgt attgttgctg gcaagtgggc tgataaatca ctttgcgatg gtgggcgcaa
     5101 ctgcggttaa aagtctggtt gcggtgcatg aagtttgcgg atttttgtta ctggcatgct
     5161 ggctcggctt tgtgctgatc aatgccgttg gggataatgg tcaccactat cgcattcgtc
     5221 gtcaggggtg gctggaacga gcggcaaaac aaacgcgatt ttatttgttt ggcattatgc
     5281 agggggaaga acatcctttc ccggcaacaa cacagtctaa atttaatccc ttacagcagg
     5341 tcgcctatgt tggtgtcatg tatggattgc tgccgttgtt actattgacg gggctgttgt
     5401 gtctctatcc gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg ttattgcagg
     5461 cgcattttgc tctggcattt ataagcctct tttttatctt cggtcatctt tatctttgca
     5521 ccacggggcg tacgccacac gaaaccttta aaagcatggt cgatggctat caccggcact
     5581 aacgaatgat cattacacga gcagacctgc gagaatggcg tatcggagcg gtgatgtatc
     5641 gctggttcct gcgccatttc ccacgcggcg gtagttatgc cgatattcgt catgcactga
     5701 ttgaggaagg atatacggac tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc
     5761 tggcggatga aaacttcgcg catcaggaag tttcctctat gcaaaaactg gccacagacc
     5821 ccggagagat accattttgc agtcagtttg cccgcagcga cgatcatgct cgcatcggct
     5881 gttgtgagga taacgcccga atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg
     5941 gttatagcgt gcggattgga agtgtcggct ttaacagtca cattggcagc tccggtgaac
     6001 gcgcacgggt tgctgtaacc ggtaattctt cgcggataag cagcgctgga gatagtagtc
     6061 ggattgcgaa taccggaatg cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg
     6121 ccagtaatgg tgacctggtg caaatcgcca gctttggcgc aaacgccaga attgccaaca
     6181 gcggcgacaa tgtacacatc attgccagcg gtgaaaattc aacggtagtg agcacgggcg
     6241 tggtcgactc aattattctc gggctagggg gaagcgccgc actggcatat catgacggcg
     6301 aaagagttcg cttcgccgtc gccattgaag gtgaaaacaa tattcgaacc ggagtccgct
     6361 atcgtcttaa tgaacagcat cagtttgttg agtgctgata gctttacctt taatcaaacg
     6421 aaagacgccg acgagcacgg gatgcaggct ttacgaccgc ccgagccatc gcctcaccaa
     6481 tttctgcaca tgccgtaagt ccctgaacga aaggctggtc gtgcatcaac cagggcaacg
     6541 cgccgaacgc tacgcgtcca cgaatatctt cgggttgctg taacgtataa tcttcgccaa
     6601 catcagggat ttcagatccc cgcttcacgt aaggccagta gtcgacgaat agactctgaa
     6661 gggattgccg cataattatc gatgaaaacc ctggcaaggc ctttactgaa ccgtttatgg
     6721 tcgtgttcat tcagatgagg aacaatttcc tgtacggctt catgcaggcg cagaatgaca
     6781 taacgccagg gaacagtatg tttttctcgt ttattgcgtt cgacttcctg gagatttttt
     6841 tctgcccagt caaatgggtc gcgttgtttg cgctcggcaa accaggcttg agcaaaggaa
     6901 tcgacattca ggctctctaa ggctatgcgt tggctccagt ctggatcagc aaacttgatt
     6961 tcctctacta tcaatctaaa tacccgatcc aaaaggccct cttcaccttt ttgaatctca
     7021 gcatttaatg cctgatcggt gacgatgtgt aagggctcgt agggaatagg gcaatagaaa
     7081 tcggcttcgg gtaaaatacc cgtgcgcgac atcaacgtaa tatttagctt ttcacttgcg
     7141 ttatcgcggt gaaaaacgac gtgttgttta tcatcttcaa tgaacgaacc atgctgaata
     7201 gccactgcca ttgccgcatc cagtccgctc aaggatgttc ccataatacc cacgttacac
     7261 gcatcgacct ttgcttccat caggcctgac cacgggctgg gaaaatacgt tctggttgct
     7321 tcttcttcat caggccagac gtgacccgtg gcaatcaccg ctaaatcaaa cgtctcgctg
     7381 ggtaaatcct gatttgtagc gagcatgacg ccagcatttg taatttgcag atcggtaacc
     7441 tggcatgatt cataaacagc cactgcaaat ttttgctgtc gtgcctggtc tactaatcgt
     7501 aaaaattgat cgcggaaata ttcgcccagc agaattcgcg gtaaaaactg acgatcgtgc
     7561 aaggtttctt ttttaacgcc ataacgctgg agatggctgg cttcttgctt ttgtagccat
     7621 tcgagatacg tacaattaat cggcggtatt tcaatactgg caatatttgc cagcatcatt
     7681 tttgagtttt cctcatcact gtatggcatc ccgacaccgg cctcgtcagc ctgctcgaag
     7741 atagaaatag aaagtggagt ttgttgctgt agaagcgaga ataaggtgta gatccccgta
     7801 ggcccggcac ccacaatagc aatttttttc attgttactt acctgtacgc attctttcga
     7861 atttagtgtt atcagataag tttggttgag atttttagaa tttgagaaat ttcagacagg
     7921 gatgaaaagg gcgctaaccg cccttgatta aattctgtca ggcgagtttt gcctgattga
     7981 tttgactaag tggctcattg acatcgaaaa ctttggcgac aacttcctca acgccgagat
     8041 ttttcagtcg ggcagtatgt ttttccagat aggcaagggc gcttttttca tcagtgaaaa
     8101 ggtagatccc accggcttcg tggttctttt cactttctgt ccataccttc cacagaaaac
     8161 caggttcctg attaatcgac tcagcaagtg gtttaagctg ctcagccatt gcgtcgccaa
     8221 atgggccatt aaaagcaaaa tgaagttgta acaaagttgc catagcgatt ctccttctga
     8281 taacgcatac gaagacagtg taaatctttt aacgtattgg atacagagaa gaaacagagg
     8341 gaaaaaggaa aagaccagat taagaaaatc tggtgcgtcc gagtggactc gaaccaccga
     8401 cccccaccat gtcaaggtgg tgctctaacc aactgagcta cggacgcagg atggtgcgtt
     8461 caattggact cgaaccaacg acccccacca tgtcaaggtg gtgctctaac caactgagct
     8521 atgaacgcaa cgttgtaggt gacaacgggg acgaatatta gcggcagagt ggaaaggtgg
     8581 caagaggcaa aacgtaattt tctgcgctat ttcgaccgtt tgcagagctt ttaagcaaat
     8641 tggctatatt ttgttgattt gcaagggcga tttttattca ggatcgcatt tacatctgat
     8701 acaacccaat aattcgtgta tcagagttta aacagagttg tttgccacca tctcaaatct
     8761 gtcgtggata aatcctccgt agcctttaca cttaggtagc aataaaggca cacagatgca
     8821 gcaaaggaat aaatcatggg atctgatgcg aaaaacttga tgagcgacgg gaatgtgcaa
     8881 attgttaaga ccggcgaggt ccttggcgcg acgcaactta ctgaaggcga gttgattgtt
     8941 gaagctggcg gaagagccga aaataccgtg gtcacggggg ctggctggtt gaaagtggca
     9001 accggtggga tcgccaaatg cacacagtac ggtaacaatg gcacgctatc ggtcagcgac
     9061 ggtgccattg ccacagatat tgttcagtcc gagggaggcg caattagtct ctctacgctc
     9121 gctacggtta atggccgcca tcccgaaggt gaattcagcg ttgatcaggg ttatgcctgc
     9181 ggtttgttgc tggaaaatgg cggtaacctg cgtgtactgg aaggacatcg cgcggaaaaa
     9241 atcattctcg atcaagaggg cggcctgttg gttaatggga caacctcagc ggtcgtggta
     9301 gatgaaggtg gtgaattgtt ggtgtatcca agtggagaag ccagcaattg tgagattaat
     9361 cagggcggcg tttttatgct ggccgggaaa gccagtgata cgttgcttgc tggtggcacc
     9421 atgaataatc tcggtggtga agactctgac actattgttg agaatggatc catttatcgt
     9481 ctggggacgg atggccttca gctctacagt tccggtaaga cgcaaaacct gtcggtgaat
     9541 gtgggggggc gggctgaagt gcatgccggt acgctggaaa atgcggtaat acaaggtgga
     9601 acagtgatcc tgttgtcacc caccagcgcg gacgaaaatt ttgtcgtaga ggaagatcgc
     9661 gcaccggttg aactgaccgg gagtgttgca ttactggacg gcgcttcaat gattattggt
     9721 tatggcgcag agctgcaaca atcaacgatt actgtacagc agggcggtgt gttgattctc
     9781 gacggcagta cggcaaaagg tgacagcgtc actttcattg ttggtaacat caatctgaat
     9841 ggcggaaaac tgtggctgat cactggtgcg gcaacgcatg tgcaactgaa agtgaaacgc
     9901 ctgcgcggag agggagcgat ttgcctgcaa accagtgcga aagaaatttc acctgacttc
     9961 atcaatgtga aaggggaagt taacggggat atacgcgttg agataacaga tgccagtcgg
    10021 caaactctgt gtaacgctct gaaattacag ccagacgaag acgggattgg cgcaacgctc
    10081 cagcctgcgt aaaaacacag gcgcaacccg tgaaggttgc gccttgtctt tattagcggg
    10141 acgctcgttg cagaatgatg gctgacggca tacgttgcag gaaccgcata cgcagcatca
    10201 tcataatggc tgccgatgtc aggccgataa taaagcctat ccagaagcct gctggcccca
    10261 taggttcaac gaccagatcg gtcagtgcca gaatatagcc gcttggcaag cccagcaccc
    10321 agtaagctgt aaaggtgata tagaaaatgg aacgcgtatc tttataacca cgcaaaatcc
    10381 cactgccaat cacctggatt gagtcagaaa tctgatatac cgccgccagc aacatcaaat
    10441 gcgcagccag cgttacaacc tcgggattgt cgttgtacaa cagggcgatt tgctcccgca
    10501 gtgaaaccgt gaaaatggcc gtcagggttg ccatacagac acccaccata agcccggtcc
    10561 gcgcagcggt ttgcgcatcc agcgttgagc cctgacccag acgataacct acgcggatag
    10621 ttaccgctgc cgccagcgac attggaagca caaacatcag tgaactaaag ttcagggcaa
    10681 tctggtgtcc tgcgacatca acaataccga gcggagacac taacagagcc acgacggcaa
    10741 acagtgtcac ttcaaagaac agtgccagcg caatcggcaa accgagttga atcagtcgtt
    10801 tcataaccgc aggctcgggc ttagcggtgc ctttttcgtt acgaatatcg cgcatggagc
    10861 gggcgcgttt aatgtaagaa accatggcaa ggaacatgac ccaatacacc gccgcagtag
    10921 ccacgccaca accaacgcca ccgagctcag gcataccgaa atgaccataa ataaagatat
    10981 agttcaccgg gatgttcacc agcaggccga taaagcccat taccataccc ggcttggttt
    11041 ttgccagacc ttcacattgg ttacgggcaa cctggaagaa cagatatccc ggcgcgcccc
    11101 acaacaacgc acgcagataa cccacggctt tgtccgccag agccggatcg atgttttcca
    11161 tgtagcggat aatgtaacct gcattccaca gcaccagcat aatgagaacg gaaacaaaac
    11221 ctgccagcca gaaaccttgt cgcacctgat gcgcaatgcg ctcacgtcga ccggaaccat
    11281 ttaattgcgc gataaccggc gttaatgcca gcagcagtcc gtgaccaaag aggatcgccg
    11341 gaagccagat agaagtaccg atagcgaccg ccgccatgtc ggtggcacta tagccgcccg
    11401 ccatcacggt atcgacaaaa cccatcgcag tttgggcgat ttgcgcgaga atcaccggga
    11461 ttgctaatgc taataacaga cgcgcttcac tgatatactt ctgcacgtga acacctttta
    11521 tttgtagtta tatgagagac taaaaagccg cctgaatggg cagcaaaaag aagaagcagg
    11581 ggaaattcag tctattgtag cgaggattta ttaattctcc agtgaaaaaa tagccattct
    11641 taccccttca ctggtaaggg cgttttccac ctgctattgt gctgaacaga atgtcttaac
    11701 tgatttcagg agttgtaagt atgtttacgg ggattgtaca gggcaccgca aaactggtgt
    11761 cgattgacga gaaaccaaat tttcgtacgc atgtggtgga gttacccgac cacatgctgg
    11821 acggcctgga aaccggtgct tccgtggcgc ataacggttg ctgcctgacc gtgacggaaa
    11881 ttaacggcaa ccatgtcagt tttgacctga tgaaagaaac gttacgcatt accaatcttg
    11941 gcgatttaaa agtgggggat tgggtaaacg ttgagcgtgc ggcgaaattc agtgatgaaa
    12001 ttggcggaca cttaatgtca ggtcatatta tgaccaccgc tgaagtggcg aaaatattaa
    12061 cctcggaaaa taatcgccag atctggttta aagtccagga tagtcagttg atgaaatata
    12121 tcctgtacaa aggatttatt ggcatcgacg gtattagcct gaccgtcggc gaagtcacgc
    12181 caacgcgttt ttgcgtccat ttaattccgg aaacactgga acgcacgact cttgggaaga
    12241 aaaaacttgg cgcacgcgtc aacattgaaa tcgatccaca aactcaggca gtggtagata
    12301 cggtagaacg tgtgctggcg gcacgagaaa atgccatgaa tcaaccaggt acagaagcct
    12361 gatctgaact cgcccccgaa gagggggcga tagaatagcc tttagcgagc gactcgaagg
    12421 ccgttttcaa caccgcgtga gaacaccacc tgccagagct gaatatcacg ggcgcggaaa
    12481 gcacctgcgc aggcattcag ataataggta aacatccgtt taaagcgttc actgtagtta
    12541 tccgcaattt ctggccatgc ggcgaggaat cgttcatacc acgccatcaa cgtagtatcg
    12601 taatcagcac cgaagttatg ccagtcttcc atcacaaagt ggggttcgct ggattgagca
    12661 atctggcgta cagagggcag gcaaccgttc ggaaaaatat atttattaat ccagggatca
    12721 acattcagat cggttttttt cgaaccgata gtatggagca ggaatatacc ttccggtttc
    12781 aaattacgat ccaccaccgc aaaataggta tcgtaatttt tcggtccgac gtgctcgaac
    12841 atccccacag aaacaatacg atcaaactgg tcgttcaggt cacgataatc ttgcagcaaa
    12901 atggtgacat ccaggccttt acagcgttcc tgagccattt tttgctgttc ggcagaaatg
    12961 gtgacgccga ccacgcttac gtcatagtta gatgccatgt agtgcgctag tccgccccag
    13021 ccgcagccaa tatccagtac gcgcatccct ggttttaact gcaatttttc acaaatcatt
    13081 ttgagcttcg cctgctgggc agattccaga ttatcggcat ctttccagta agcgcaggaa
    13141 tattgcatga agggatcaag catgcggctg aacaagtcat tacccaaatc gtaatgctcc
    13201 ttgccgacta tccaggcacg ttttttactc tgcagattga agagacgagc gccggcaata
    13261 cgcagcgtgt ctttgaaatg atgggggagt tggttctcga gacctgcgcg taagactttg
    13321 ctaaaaaaca tatccagtcg gtcacattcc caccagccat ccatataact ttcgcctaac
    13381 cccaaagagc cttcctgcag aacgcgttta aaaaaatcgg ggtttttcac acgaatatcc
    13441 gccggggcag aaccgttaat ggctataccg gcacggctaa gtaattcgtt ggcgatacgg
    13501 taccagttgt catccggtac actgacttct tctatacacg atgaactcat agtttctcca
    13561 tcactggtcg gtgatcagaa ccttaaaaca gcgtagacgc tttttgggct ttgtgagaaa
    13621 tctcgcagaa aaaaccgcga gtctataatc cgacgtagaa cagagggaaa ggaggaaccc
    13681 ttgccgaaat gaccatccat agtgagtcgg gaacgatcct gtccccgtaa aaacataata
    13741 tttatcgtag ctttaacaac tcgaaattag tataggccgc taatttcaat catacaacaa
    13801 aattttgtta gcagcctaag tataagtata tcgatataga tcagtgtgat tcgctatgag
    13861 cgacttcggc attgccatga ttcgggcagt caacttcttc acaacgttgc atcatgtaac
    13921 ccagcgcgac cagcactact gttgataaca tcacgctggt ggtggtgagc aatggcgtgc
    13981 tgatactaat aagccaggaa actaccagac ttgcgaggaa gcacagaccc agttgcagag
    14041 tgttctgcaa cgctgcggcg cgaccggttg catgtgggaa gggacgcagc gcctgggcga
    14101 caacaatagg gtagatcgcg ccattggcaa tcgccatcac acagaatggg atcaggattt
    14161 cgaccagcga cacatggcta atgaagcccg cagcccaggt cgcaatgacg ctgacagcaa
    14221 acagcaccag caaccacggt aataactgct tgccttgcca tttctgcagt gcggcgcgac
    14281 agccataacc accaatcaga aacgcgatag tttgcgggac ataacttaaa ccaataactg
    14341 ccgggctgta gcccatttca ctaaggatga acggtgaacc ggtcagccat gcaaaaaaac
    14401 tggctgaaca ggctgcgtat atcagcacgt tgccgcgata ggttttagaa cgtagcaggt
    14461 cggtaaaggt cagaccatcc tgactattgt tacgggcctt cgtcgtgggt ttgagccaga
    14521 aaataggcag aatcagcacc acggtaatgg caaacagggt ggcgaaaatc gcctgccagg
    14581 aaaaatggac cagcagccag cttcctaaca ggggagccag tgccggagac agacccacca
    14641 gcggcatgat ggtcgcaaaa atacggttga ctttctgtga aggataataa tctgtcacta
    14701 atgcttgcca gataaccgcc gcggcgcaga cccccacagc ctgtacaaaa cgcaatacca
    14761 gcagcgtggc ggcgttttct acccacagca tccccagact acctaacgca aaaattgtca
    14821 ggccgattaa taacaccggt ttacgaccat aacggtcgga gagtggcccc cacagaagct
    14881 gggctgcggc aaaaccggca aggaacagac taaggctggc actgacagca gacgcaggcg
    14941 tttgcaggtc ggcctgtatg gcggcgaaag caggcagata catatcggtt gccagaaaac
    15001 cgagtacgct caaacccgcc agccagacta aaaatctttt cccaggttgc atgtatattc
    15061 tctaaatctc tcaattaaac accgccgcaa agtgtaggga gtgaaattag tgttgtgaaa
    15121 cggtaatatt tgatgactgg tttcaaaaat tttgcaggca gactatgtgg tcagaatatt
    15181 cactcgaagt tgttgatgcg gtagcgcgta atggtagttt tagcgctgcg gcacaggagc
    15241 tgcatcgtgt tccttctgcg gtcagctata ccgtgcgtca gctggaagag tggctggcgg
    15301 taccgctctt tgaacgacgg caccgtgatg tggagctgac cgctgctgga gcgtggtttc
    15361 tcaaagaagg gcgctctgtt gtcaaaaaaa tgcagatcac ccgccagcaa tgtcagcaga
    15421 tagcgaacgg ctggcgcggt cagttagcta tcgcagtgga taatattgtc aggccagaac
    15481 gtacacggca gatgatcgtt gatttttatc gccattttga tgatgtcgaa cttcttgtct
    15541 ttcaggaagt gttcaacggt gtctgggatg cgctttccga cgggcgcgtg gaactggcaa
    15601 ttggcgcaac acgcgcgatt ccggtaggcg gtcgttatgc cttccgggat atggggatgc
    15661 taagctggag ttgcgttgtt gccagtcacc acccgctggc gttgatggat ggcccgttca
    15721 gcgatgatac gttgcgcaac tggccgtcgt tggtgcgcga agacacctcg cgaacgctac
    15781 ctaaacgtat tacctggttg ctggataacc aaaaaagact tgtcgtacct gactgggaat
    15841 catcggcaac ctgtatctcg gcaggattat gcatagggat ggtgccaaca catttcgcca
    15901 aaccgtggct taatgagggg aagtgggtag cacttgaact ggaaaacccc ttcccggatt
    15961 cggcatgttg tctgacatgg cagcaaaatg atatgtcgcc tgcgttaacc tggctgcttg
    16021 agtatctcag cgatagtgag acgctgaata aggagtggct gcgggagccg gaagagactc
    16081 ccgcaacggg tgattaacga cgatagtcgc ggaacgggcc gtcagccacg gagcggcgtt
    16141 caatcaaacg cggatgcact tcaatggact gcggttcttc acgtttgttg acgatacgat
    16201 ccaacagcat gttgaacgct gtttcaccca gcgaatcttt tggctgatgg atcgtggtca
    16261 gcgccggcgt aaaatagcgc gcgttgcgca cgttatcata accgatcagc gaaacatcct
    16321 gcgggacgcg caggcccatt tcatcagcag cacaaagtgc gcccattgcc atgatatcgc
    16381 caccacagaa gacggcagta gggcgatgcg gctgcgacag gatttgctgc atggcgcgat
    16441 aaccggattc aggttcaaag tcaccctgca caatccagct ttccggcacc ttgatcatcg
    16501 cttcttccat cgccttcata aaaccggcaa ggcggcctgc gccggtgtta cgttccagcg
    16561 ggccggggat gacgccgatt tcgcggtgac cgcgttcaat cagataacgc ccggccatgt
    16621 agccgccttc gaacgcgtta tcaatgaccg catcggtgaa gtcagctttt gcttcacccc
    16681 agtccatcac caccattggg atatggcgat actcttccag catcgccagc aacggctctg
    16741 ggtactcaga acacatcacc agcagaccat cgacgcgttt ttgcgccatc atcgacagat
    16801 aagcccgctg tttctcaaga ttgttccacg cattacccag aatcagggtg taacctttct
    16861 ggaagcaatt tttttcaact gcttcaatga tctcggcaaa ataggccgct tcgctgctgg
    16921 tcgccagcaa accgatagac ttggtgtggt taaccttcag gctacgcgcc accgcgctag
    16981 gggagtagtg taattcttta atcgctgccc acacggcgtt gcgcgtttct tcagcgacga
    17041 aacgtgtttt gttgatcacg tgtgacacag ttgtagtgga aacgtttgct cgtttcgcta
    17101 catcttttat tgttgccatt ccatttcact ccagacccta acttcagctc ctgcaaaatc
    17161 gcaaggtaaa cgtttgcctt tacacacctt acgcacaaca aaagtgttgc ggtacgccgg
    17221 aaaaacgaca cggagcgtca ggaaggggtc aatggccagt acgcaaatag tgtaagcgtg
    17281 gaattttggc cgatcttgac gaaaagggga agtggtaaaa acacttatca ttataaatga
    17341 cgggagattt tttcatcaca gtgtgtaaaa atgcgtaata tcactttttg tgtggtaata
    17401 aaaaaggaga aaacttgatg agcaccgacc ttaaattttc actggtaaca acggttatcg
    17461 tcctcggttt gatcgtagcc gtgggtttga ctgccgcgct gcactgattt cctctcgagg
    17521 gagcgcgttc tccctcgcat ctccttcctg tttgttagca aaatggcaaa agtgttttct
    17581 ctttttgtta ttttcatttt tttgtgattg ctgtcatgct ttcgccactt taatgaatat
    17641 cgcgtaatga cgcgaagagg tggagataag gcatgaaaat taactatccg ttgctggcgc
    17701 tggcgattgg cgcgtttggt atcgggacaa cggagttctc gccaatgggc ttgttgcccg
    17761 tcattgcgcg cggtgtggat gtctcgattc ccgctgccgg aatgttaatc agtgcctatg
    17821 cagttggcgt aatggtgggc gcgccgctga tgacgcttct actttctcat cgtgcccgcc
    17881 gcagtgcgtt gattttcctg atggcaattt tcacgctagg caacgttctt tccgccatcg
    17941 cgccggatta tatgaccctg atgctttcac gtattttgac cagcctgaat cacggagcat
    18001 tttttggttt gggttcagtc gtggccgcaa gcgtggtgcc aaaacataaa caggccagcg
    18061 cagttgccac tatgtttatg gggttaaccc tggcaaatat cggtggcgtg ccggcggcga
    18121 cctggttggg tgaaaccatc ggctggcgga tgtcatttct ggcaacggcg gggctgggag
    18181 tgatttcaat ggtaagtctg ttcttctcat tacctaaagg tggtacaggg gcacgacctg
    18241 aagtgaaaaa agagctggcg gtattaatgc gtccgcaggt gctgtctgca ttgctgacga
    18301 cggtactggg agctggtgca atgtttaccc tctacaccta tatctctccg gtactgcaaa
    18361 gtattaccca cgcaacaccg gtgttcgtca cggcaatgct ggtgctgatt ggtgtcggat
    18421 tctctatcgg taactatctc ggcggcaaac tggcagatcg ttcagttaac ggcacgttga
    18481 aaggcttttt gttgctgctg atggtgatta tgctggcaat cccgttcctg gcccgcaatg
    18541 agttcggcgc agctattagc atggtggtgt ggggcgcagc aacctttgcg gttgtacctc
    18601 cgttacagat gcgcgtgatg catgtcgcca gtgaagcgcc aggtctgtct tcatcagtca
    18661 atattggtgc ctttaatctt ggaaatgcgc tgggagcagc tgctggtggt gcggtaattt
    18721 ccgctgggct gggatacagc tttgtgccgg tgacgggggc gattgtcgcg ggactggcat
    18781 tattgctggt gtttatgtca gccagaaaac aacctgaaac agtttgcgtt gctaacagct
    18841 aattaaacaa aaacgcagaa ggataacctt ctgcgttaca gttgctgaca acgtgcgatt
    18901 tatcttgccg aatgcgctgt taacgcctca tccgcgttgg catggtaagc gaagcgcatc
    18961 aggcaatgct gcatttgcca tcagttatta tgcagcgaga tttttcgcta cgaattccca
    19021 gttcaccagc gcccagaagt gctccagata gccaggacgt gcattgcgat agtcgatgta
    19081 ataagcgtgt tcccagacat caacggtcag cagcggagtc gcatcggtgg tcagcggagt
    19141 acccgcgtta gaggttgaaa cgatagccag tttgccatcg ctgtttttca ccagccaggt
    19201 ccagccagaa ccaaagtttt tgatcgctgc atcagtaaac tgcgctttga aatcggcaaa
    19261 gctgccaaaa gatgcggcga tagcttcagc gacttttcca gtcggttcgc caccggcgtt
    19321 cggtgccagg cagttccagt agaaagtatg gttccagacc tgagctgcgt tgttgaatac
    19381 gccaccttca gagctgcgaa taatctcttc cagtgattta ccttcaaacg cggtaccttt
    19441 aatcaggttg ttcaggttag tgacataagt ctgatgatgc ttgccgtagt gatactcgat
    19501 agtttccgca gaaatgtgcg gtgccagagc atctttagca tatggtagtg caggtaattc
    19561 gaatgacatt gctactctcc tttattatta atttgcatac gtacaatagc cttattgtgc
    19621 gtatggatag ggtagcaaat taaaaggtga gacaaaagat gaacttaccc tgttgccgta
    19681 acaacagggt aaagttttag cgaagtgttt ttggggtcat tacccgacga gcgccaacat
    19741 agtggcgctg ccagtagtct tcactgagag aagtgatttg aatttcctga cctgtgcgcg
    19801 gtgactgaat aaatttgccg ttgccgacat acacgccgac atgatcggct gtaccgcgtc
    19861 cctgagtacg gaaaaagacc aggtcgccgt ttttcagctc actacgttcg ataggtcctg
    19921 catcacgcag gtgatacatt tcattcgccg tacgcggaat acgaattttc accaaatctt
    19981 tataagcgta ataaaccagg ccgctgcaat caaaaccggt acgcggtgag ctgccgcccc
    20041 aacgatatgg cttaccaatt tgctgcatca gtttattcat tgccactttc gtggcttttt
    20101 gcaccttcgc tttgtgcgcg tcggcaaggg ttcctgacgc ggcatttttg actttcacac
    20161 aatgcgattt atagccctta cgtttggtac acttttcggt aaagctggct gaggccgttt
    20221 tattgacggc gtgtttgctg cgagttttgg cgtttttaga agcggtaatg gaagattttt
    20281 taacagaaga agcggttttg cttttggtca tacttttact ggcggctttt ttcgctgttt
    20341 tctggctggc tttctgggtc tttttggtgg ttgccgtgct tttcttttta tctgcttttt
    20401 tggtgatatg agtggtagca gaactctccc tggcttgctt tgaagcatgg gccataggcg
    20461 ttaaaggcag ggtggtaaaa agtaaagcac agagcgtgat cgaaatacgg tttatccgcg
    20521 ccactgagcg tccctctgtt aaggcaggtc tatcatgcct gcgtctgatt tacaaaactt
    20581 gatgattcta atgcatagaa gaagagaaag ttaatagctt ttattgttaa aaaatacgtt
    20641 tcgttggcga gcgcaaaaaa atgcagcagt gaaagctaaa aatggttata aatacgccag
    20701 tttgccgcgc atcagagaag aaacgaatat ttgaaatgca acaaatagag caaagggata
    20761 aaatagcaaa agcgtgatga aaatgttatt ttccgatgcc gcactgaccc gctacaatgt
    20821 cagattactg ccgtaaagaa gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa
    20881 tccaacgcca gattgctgaa aacccgatcc tgctgtacat gaaaggttca ccgaaactgc
    20941 cgagctgcgg tttctctgcc caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg
    21001 cctatgttga tattctgcag aatccggaca ttcgtgcgga actgccgaaa tatgctaact
    21061 ggccgacctt cccgcaactg tgggttgacg gcgagctggt cggcggttgt gatatcgtga
    21121 tcgaaatgta tcagcgtggc gaactgcagc agctgatcaa agaaactgcc gctaaataca
    21181 agtctgaaga gccggacgcg gaataatccg tcctgcaata ctgacggatg cgaacgggca
    21241 tccgtcagtc ctttatccgg tgcgccgtaa aacctcgtcc ttcagggcgg ggatataagg
    21301 cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtactggcgg atgacggata
    21361 ttggcgcacc ttcgcagcta ctggcaaaat agccaggact ccacaaaacg cctttgtagt
    21421 aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctattcgat atgcctttga
    21481 ggctgtttac cagactggat atcgccagtt tgggaggata gttaattaac aaatggacgt
    21541 gatctggttc gccatccatt tcaaccagtt cagctgcaaa atcagcacat acatttgaaa
    21601 agtaagtgcg tagtttttct gtcgtgtcat ggtcaaaaat ctggcgtcgg tatttggtga
    21661 caaagaccag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg atatcggttt
    21721 cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca tttaaattcc
    21781 agttaagacc cggtggtcaa caggagcgtg aaatgaggcg cttcgcaggc gcatgtcgtt
    21841 tcgttttcaa tcgtgctctg gcgcttcaga atgagaatca tgaggccggt aataaataca
    21901 tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaacgccact gaaacgcaat
    21961 ggcttaaaga ttcgccctca cagccattgc aacagtcact gaaagacctt gagcgggctt
    22021 acaaaaactt cttccagaag cgggctgctt ttccccgatt caaaaagcgg ggacagaatg
    22081 ctgcattccg ctacccgcag gacgttaagc tcgatcagga aaacagccgt atttttctgc
    22141 cgaaacttgg ctggatgcgc taccggaata gccgtcaggt cacgggagtt gtgaaaaatg
    22201 tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa agtgaagtat
    22261 caactccggt tcacccttca gcatcaatga tcgggctgga tgctggcgtg gctaaactcg
    22321 ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcaaaaa aaccagaaga
    22381 cgctggcgag acttcagcga cagttaagcc ggaaggtcaa attcagcaac aactggcaga
    22441 agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc agggactacc
    22501 ttcacaaagt cacaacgacc gtcagcaaaa accacg
//
LOCUS       DAZSNG010000060        22412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZSNG010000060 DAZSNG010000000
VERSION     DAZSNG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.061"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..2682
                     /locus_tag="ACHW4Z_002447"
     CDS             82..2682
                     /locus_tag="ACHW4Z_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR8882047.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYIAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHW4Z_002448"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHW4Z_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHW4Z_002449"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHW4Z_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHW4Z_002450"
     CDS             4302..4817
                     /locus_tag="ACHW4Z_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8882048.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHW4Z_002451"
     CDS             4783..5520
                     /locus_tag="ACHW4Z_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR8882049.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHW4Z_002452"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHW4Z_002452"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR8882050.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHW4Z_002453"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHW4Z_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR8882051.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHW4Z_002454"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHW4Z_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR8882052.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHW4Z_002455"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHW4Z_002455"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR8882053.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHW4Z_002456"
     CDS             10827..12734
                     /locus_tag="ACHW4Z_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8882054.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHW4Z_002457"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHW4Z_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR8882055.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHW4Z_002458"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHW4Z_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR8882056.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHW4Z_002459"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHW4Z_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR8882057.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHW4Z_002460"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHW4Z_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR8882058.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHW4Z_002461"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHW4Z_002461"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR8882059.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHW4Z_002462"
     CDS             complement(17402..19162)
                     /locus_tag="ACHW4Z_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8882060.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHW4Z_002463"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHW4Z_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR8882061.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHW4Z_002464"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHW4Z_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR8882062.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHW4Z_002465"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHW4Z_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR8882063.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="ACHW4Z_002466"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="ACHW4Z_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR8882064.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5257 c         5873 g         5561 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatattgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAZSNG010000061        21772 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZSNG010000061 DAZSNG010000000
VERSION     DAZSNG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21772
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.062"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..239
                     /locus_tag="ACHW4Z_002467"
     CDS             <1..239
                     /locus_tag="ACHW4Z_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR8882065.1"
                     /translation="KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQ
                     LVKLPTRRGRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(277..1275)
                     /gene="fbp"
                     /locus_tag="ACHW4Z_002468"
     CDS             complement(277..1275)
                     /gene="fbp"
                     /locus_tag="ACHW4Z_002468"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR8882066.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1451..2824
                     /gene="mpl"
                     /locus_tag="ACHW4Z_002469"
     CDS             1451..2824
                     /gene="mpl"
                     /locus_tag="ACHW4Z_002469"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR8882067.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2907..3458)
                     /gene="yjgA"
                     /locus_tag="ACHW4Z_002470"
     CDS             complement(2907..3458)
                     /gene="yjgA"
                     /locus_tag="ACHW4Z_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR8882068.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3552..4904
                     /gene="pmbA"
                     /locus_tag="ACHW4Z_002471"
     CDS             3552..4904
                     /gene="pmbA"
                     /locus_tag="ACHW4Z_002471"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR8882069.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            5087..5469
                     /gene="cybC"
                     /locus_tag="ACHW4Z_002472"
                     /pseudo
     CDS             5087..5469
                     /gene="cybC"
                     /locus_tag="ACHW4Z_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5514..5978)
                     /gene="nrdG"
                     /locus_tag="ACHW4Z_002473"
     CDS             complement(5514..5978)
                     /gene="nrdG"
                     /locus_tag="ACHW4Z_002473"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR8882070.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(6136..8274)
                     /gene="nrdD"
                     /locus_tag="ACHW4Z_002474"
     CDS             complement(6136..8274)
                     /gene="nrdD"
                     /locus_tag="ACHW4Z_002474"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR8882071.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8668..10323)
                     /gene="treC"
                     /locus_tag="ACHW4Z_002475"
     CDS             complement(8668..10323)
                     /gene="treC"
                     /locus_tag="ACHW4Z_002475"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR8882072.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10373..11794)
                     /gene="treB"
                     /locus_tag="ACHW4Z_002476"
     CDS             complement(10373..11794)
                     /gene="treB"
                     /locus_tag="ACHW4Z_002476"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR8882073.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11913..12860)
                     /gene="treR"
                     /locus_tag="ACHW4Z_002477"
     CDS             complement(11913..12860)
                     /gene="treR"
                     /locus_tag="ACHW4Z_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR8882074.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            13045..13098
                     /gene="mgtL"
                     /locus_tag="ACHW4Z_002478"
     CDS             13045..13098
                     /gene="mgtL"
                     /locus_tag="ACHW4Z_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR8882075.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            13239..15935
                     /gene="mgtA"
                     /locus_tag="ACHW4Z_002479"
     CDS             13239..15935
                     /gene="mgtA"
                     /locus_tag="ACHW4Z_002479"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR8882076.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(16141..16527)
                     /gene="ridA"
                     /locus_tag="ACHW4Z_002480"
     CDS             complement(16141..16527)
                     /gene="ridA"
                     /locus_tag="ACHW4Z_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR8882077.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16600..17061)
                     /gene="pyrI"
                     /locus_tag="ACHW4Z_002481"
     CDS             complement(16600..17061)
                     /gene="pyrI"
                     /locus_tag="ACHW4Z_002481"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR8882078.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(17074..18009)
                     /gene="pyrB"
                     /locus_tag="ACHW4Z_002482"
     CDS             complement(17074..18009)
                     /gene="pyrB"
                     /locus_tag="ACHW4Z_002482"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR8882079.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(18013..18147)
                     /gene="pyrL"
                     /locus_tag="ACHW4Z_002483"
     CDS             complement(18013..18147)
                     /gene="pyrL"
                     /locus_tag="ACHW4Z_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR8882080.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18428..18823)
                     /locus_tag="ACHW4Z_002484"
     CDS             complement(18428..18823)
                     /locus_tag="ACHW4Z_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8882081.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18955..19668)
                     /gene="bdcA"
                     /locus_tag="ACHW4Z_002485"
     CDS             complement(18955..19668)
                     /gene="bdcA"
                     /locus_tag="ACHW4Z_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8882082.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19739..20332
                     /locus_tag="ACHW4Z_002486"
     CDS             19739..20332
                     /locus_tag="ACHW4Z_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8882083.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20477..20929
                     /gene="tabA"
                     /locus_tag="ACHW4Z_002487"
     CDS             20477..20929
                     /gene="tabA"
                     /locus_tag="ACHW4Z_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR8882084.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            21052..>21772
                     /locus_tag="ACHW4Z_002488"
     CDS             21052..>21772
                     /locus_tag="ACHW4Z_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8882085.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5268 a         5381 c         5745 g         5378 t
ORIGIN      
        1 tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa
       61 tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg
      121 caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc ccgagagtgg
      181 taaaggagag gccctggaaa taccccacag ccccgaaaaa gagccagtca gttgcttaac
      241 tgactggcat tactccgtgt ggagaggcgc aggagattac gcgtccggga actcacggat
      301 aaagcgttcg acatcttcaa ccatatggtc gttgccgaca aagaacgaac ggcgctggtg
      361 cagagtttcc gggatgatat ccagaatacg ctctttgcca tcgctcgctt taccgcccgc
      421 ttgttccgcc aggaatgcca tcgggttgca ctcataaagc aaacgcagtt tgccgtccgg
      481 gtggctggcg gtgcttgggt agagataaat accgcctttc agcaggttac ggtggaaatc
      541 cgcaaccagt gaaccgatat aacgcgaggt atacgggcga ttggtggatt tatcttcttc
      601 ctggcagaat ttaatgtact tcttcacccc gttcggaaac ttaatgtagt ttccttcgtt
      661 gatggagtag gttttgcctt tctccgggaa gcgcatccgt tcctggcaca ggcagaaaac
      721 gcccagcgaa ggatcgtaag taaaggcgtg aacaccgcat ccggtggtgt aaaccagcat
      781 ggtagaggag ccgtatacca cgtaacctgc cgcaacctgt ttgttaccag gctggaggaa
      841 atcttcttcc gttaccggcg tgccaacagg cgtaacgcgg cggtagatgg agaaaatggt
      901 accgacagag acgttaacat cgatgttgga cgagccatcc agggggtcca tcagcaccac
      961 gtattttgcg tgttcacagc cttcaaagac gacaatctca tcttcttctt cagaggcaat
     1021 ccccgcaacg atatcgcgtg ctttcagtgc ggctttcagt ttttcattag cgaacaagtc
     1081 gagtttttgc tgaacctcgc cctgcacgtt ctcagcaccg ctggcaccca ggatatcaac
     1141 cagtcctgct ttgttgatat cgcgatggat aatcttggcg cccagtttta ttgccgacag
     1201 caaagcagtg agctcaccgg tagcatgaga aaactcgtgc tgcttttcga caataaattc
     1261 acctaacgtt ttcataaaac tttccctgca atgtttatgg agtaaagcga ccgcaacaat
     1321 cttaacaaat aatctcaatg ttgcgctcag gtgaatcgcg ccagcaaatt gcggattatc
     1381 ctgaaatgcg tttctcactt gcccgacata tgcgtaaaat gagcggcaga ttaaaaaagg
     1441 atagtgacgt atgcgcattc atattttagg aatttgtggc acatttatgg gcggtctggc
     1501 gatgctggcg cgccagttag gccatgaagt aacgggttcg gacgccaatg tgtatccgcc
     1561 gatgagcacc ttacttgaga agcaaggcat tgagctgatt cagggttacg atgccagcca
     1621 gctcgagccg cagccggatc tggtgattat tggcaacgcc atgacccgtg gaaatccgtg
     1681 tgtggaagcg gtactggaaa aaaacatccc ttatatgtca ggtccacagt ggctgcacga
     1741 ttttgtgctg cgcgaccgct gggtgctggc cgttgccggt acacacggca aaaccaccac
     1801 cgcgggaatg gcgacctgga ttctggaaca gtgtggttac aaaccgggat ttgtgatcgg
     1861 cggtgtgccg gggaactttg aggtttcggc gcgtctgggc gaaagcgact tctttgttat
     1921 cgaagcggat gagtacgact gcgccttctt cgacaaacgc tctaaatttg tccattactg
     1981 cccgcgtacg ctgatcctca acaaccttga gttcgatcac gccgatatct ttgacgacct
     2041 gaaagcgatc cagaaacagt tccaccatct ggtgcgtatc gttccggggc agggccgtat
     2101 tatctggccg gaaaatgaca tcaacctgaa acagaccatg gcgatgggct gctggagcga
     2161 gcaggagctg gtgggtgagc agggtcactg gcaggcgaaa aagctgacca ccgatgcttc
     2221 cgaatgggaa gttttgctgg atggcgaaaa agtgggcgaa gtgaaatggt cgctggtagg
     2281 cgaacataat atgcacaatg gcctgatggc gattgcagcg gctcgccatg ttggtgtagc
     2341 gccggcagat gccgctaacg cgctgggttc gtttattaat gctcgtcgcc gtctggagtt
     2401 gcgtggtgaa gcgaatggcg tcacggtata tgacgatttt gcccatcacc cgacggcgat
     2461 tctggcaacg ctggcggcgc tgcgtggcaa agttggtggt acggcgcgca ttattgctgt
     2521 gctggaaccg cgctcgaata ccatgaaaat ggggatctgc aaagacgatc tggcaccttc
     2581 attaggtcgt gctgatgaag tcttcctgct gcaaccggcg catattccgt ggcaggtggc
     2641 agaagtggca gaagcctgcg ttcagcctgc acactggagt ggcgatgtgg atacgctggc
     2701 agatatggtg gtgaaaaccg ctcagcctgg cgaccatatt ctggtgatga gcaacggcgg
     2761 ttttggtggg atccatcaga aactgctgga tggtctggcg aagaaggcgg aagctgcgca
     2821 gtaattcggc ttcagcctga tatagcattg ccggataagg cgtttacgcc gcatccggca
     2881 aaccatactc acatcaacaa cgaaaattac ccttcattct ccgccagttc gcgcagatac
     2941 tggaaaatct ggcgtgcgga tttcggcggc ttattccctt ctttctcttt cttcgcgttg
     3001 cggatcagtg tacgcagttg ctgacgatcg gcatcgggcc acagattcaa tacttcagcg
     3061 atggcgtcat caccttgatc gatcagacga tcgcgcaggt tttccagttt atggaacagc
     3121 accacctgct ggttgtgacg gtttttcagc ttatccagcg cctgacgaat aggctctacg
     3181 tcgcgctggc gcagcatctt accgatgagc tgcaactggc ggcggcgacc ttccatctta
     3241 atacgctggg cgagctcaat agccgggcgc agatccgcat ctaacgggat cttatccagc
     3301 gcgtttttcc ccagatcaac aatttccgcg ccaaggcgtt ttagctcctc ggcatcacgt
     3361 ttaatttcac ttttactgac ccagataatt tcatcgtctt cgtcttcgat gtcatcaccg
     3421 ggaacgtcgt cgagccagtc ttcgggctgc ttagtcatct caggctcctt aaaaaaagag
     3481 gctaatgtta ccagttaaga tgcgcactga aaaacggttc tctgttagac ttcagagaaa
     3541 ctctctacat tatggcactt gcaatgaaag taatctctca agttgaagcg cagcgcaaga
     3601 ttctggaaga agcagtttcc actgcgctgg agttggcctc aggcaaatcg gacggtgcgg
     3661 aagttgccgt cagcaagacc accggcatta gcgtaagcac gcgttatggt gaagtggaga
     3721 atgttgaatt caatagtgat ggcgcgctgg ggatcactgt ttatcaccag aaccgtaaag
     3781 gtagcgcatc gtctaccgat ttaagcccgc aggccattgc ccgcaccgta caggcggcgc
     3841 tggatattgc ccgttatacc tcgccagatc cctgtgccgg cgtggcagac aaagagctgc
     3901 tggcctttga cgcaccgaat ctcgacttgt tccaccctgc ggaagtttcc ccggatgaag
     3961 ccattgaact ggcggcccgc gcagaacagg cggcattgca ggcggacaaa cgcatcacca
     4021 ataccgaagg tggcagcttt aacagccact acggtgtcaa agtttttggc aacagccacg
     4081 gcatgttgca gggttactgc tcaacgcgtc attcgctctc cagctgtgta attgccgaag
     4141 aaaatggcga tatggagcgt gattacgcct acaccattgg ccgcgcgatg agcgatctgc
     4201 aaacgccaga gtgggtcggg gccgactgtg ctcgccgtac tttatcgcgc ctgtcaccgc
     4261 gtaaactctc caccatgaaa gcgccggtca tttttgccaa tgaagtggca accgggcttt
     4321 ttggccatct ggtgggggcg atagcgggtg gctcggttta ccgtaaatct acctttctgc
     4381 tggattcgct gggtaaacaa attctgccgg actggctgac cattgaagag catccgcatc
     4441 tgctgaaagg gctggcgtcg acgccattcg acagcgaagg tgtgcgcacc gagcgtcgcg
     4501 atattattaa agatggcatc ctgactcagt ggctgctgac cagctactcg gcgcggaaac
     4561 tggggctgaa aagcaccgga catgcgggcg gtattcacaa ctggcggatt gccggacaag
     4621 ggctaagctt cgaacaaatg ctcaaagaga tgggcaccgg gctggtggtg acggagctga
     4681 tgggccaggg cgtgagtgcc attaccggtg attattcccg tggtgcagcg ggcttctggg
     4741 tagagaatgg cgaaattcag tatcccgtta gtgaaatcac catcgcaggt aatttaaaag
     4801 atatgtggcg caatattgtc accgtcggta acgatattga aacacgcagt aatatacagt
     4861 gtggttctgt gctgttgccg gagatgaaaa tcgccgggca gtaaaaaact ggcgcgacct
     4921 gccgcgctat aaactaaaaa attacacaaa atcattcgca ctgcatcgag acggaaagtg
     4981 aatgagggcc ggggagcgta ctcgcagtac gtgacccggg cgaatgagcg tagccgtcga
     5041 tgaggcagtg tgaaggatga agtgtaaata aaaaaggaag tgagcaatgc gtaaaagcct
     5101 gttagctatt cttgcagtct cctcgttggt attcagttct gcgtcgtttg ccgctgatct
     5161 tgaagacaat atggaaaccc tcaacgacaa tttaaaagtg atcgaaaaag cggataacgc
     5221 ggcgcaagtc aaagacgcgt taacgaagat gcgcgccgca gcgctggatg cgcaaaaagc
     5281 aacgccgccg aagctcgaag ataaatcacc ggacagcccg gaaatgaaag atttccgcca
     5341 cggtttcgac attctggtcg gtcagattga cgacgcgctg aagctggcaa atgaaggtaa
     5401 aaagaagcgc aggctgctgc agagcaactg aaaacgaccc gcaacgccta tcaccagaag
     5461 tatcgttaat tcctcatttc cctgttgcct gcactcaggt aacagggaac ttatcatcgc
     5521 aaatgatgca ccacctgatt gctgctgccg cgccagatca gggaagggtc ttttaaatcc
     5581 tgcacaaatt tgccgtcgac cagcacgtta atcaaatcaa caacctgcat ttgcgcagcg
     5641 ttgagttcgt cgagtttata gcctgtccac acccagatgt ctttacccgg acactcggcg
     5701 cggatgcgtt ttaccagttt cagaatatcc ggcacgtttt gcggatgcag cggatcgccg
     5761 ccggagaggg aaatcccctg gcgtttgata cgagtgtcat tcagatcttt aatgatcttg
     5821 tcttccattg ctttggtaaa tggctgacca gaatttaccc gccatgtgct tttgttatag
     5881 caaccggggc attcatgaac acacccggag acaaacaggg tgcagcgagt gccggggccg
     5941 ttgacgatgt cgacaggata gtactgatga taattcatta tttcgcctgg cttatcagtc
     6001 cgcgtgtagg ctgcatccgg taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc
     6061 ctacagccgt tgccaaatgt aggccggata aggcgtttac gccgcatccg gcatttgctt
     6121 agcgcagagt gaagattaac ctatctgccc attccccaaa tgtttaacgc ggcgcttaac
     6181 ttcttcctgc ttaccagcgt taaacggacg tgcatccggg ctacctaaat atccgcacac
     6241 gcggcgagtt accgacacac gggaggcgtc atggttacca catttcgggc aagtgaagcc
     6301 tttgctggtg cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt
     6361 attggtgccg taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcttt
     6421 caggttgtgc tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg
     6481 cgggtaaggc gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcaag
     6541 gtggaaactg ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc
     6601 agtatcgaga cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt aaagactgaa
     6661 accataaccc gtttcttctt tccactgatc cactgcctga cgcagacgtt caacaatggc
     6721 gatacctttc gcgcgaagct tctcgttgtc gtaaacatgc tcgccgctga acagcgcgtt
     6781 aatggtttcg tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat
     6841 ttcagaaaca tcatcgtcag cattcagacg tacgccacaa gcaccttcca tatagaggat
     6901 cggggcgacg cgcgctttca cgccttcgag acgagcgata cgggtcatca gtgccttacg
     6961 tgccagcacc agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc
     7021 cagagcaata cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat
     7081 ctgctcgccg ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggggtttt
     7141 aaacgaaccg gtgacgttca ctacctgatc gtagttcagg atatccggat acatgcgctt
     7201 gcttgcgcac tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt
     7261 caggccatcg cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc
     7321 gaggcctgcg atacggttac gcaggatcga ttcctgaatc aggcgcgatt cccagctggt
     7381 gcccagacca aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt
     7441 tacttcgtac tccagcgact ggaaggcatc gtagcactct ttgatggtac gagagttagc
     7501 gtagccttcg gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct
     7561 ggcggtaaca aacggcgcca gcacttcatc gatacggtta atggtggtgc cgccataaat
     7621 atggctggcg acctgagcaa taatctgcgc agttaccgcg gttgccgtag agatcgactt
     7681 cggcggttca atctcggcgt tccccatttt aaaaccctgg gtcagcatgc ctttcaggtc
     7741 gatcaacatg cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat
     7801 atcgccacgc tcatgtgcct gcaccacgtc acgcggcagc aggtgctggc gtgcatagtg
     7861 tttagccacg atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt
     7921 ggcgttttcg ttgagtaacg aggcgttggt ctgctcgacc agaccacgga tctcctggtt
     7981 caggcgaccg cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg
     8041 agccagttgt ttgtatggac ccgacatcag ctggttttca actgcggtct ggatctcgtt
     8101 gatatccacc tggttgcgcc cctgcatctg ctcgttgaca accgcggcaa cagtggcgca
     8161 ataatcggca tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg
     8221 ctctgattta aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc
     8281 tccatatttt taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc
     8341 gtttcgtttt ccggtgcttt ccgcaatcgc catccgctta tccacaacgc cgcacctttt
     8401 tcgtggggag ctgtagtagc aattatagtc gattaataca acatattggg ttgggacgca
     8461 ttttaaagtc tatatatagt gctttgcatc aaggatgttt taactttttt tgatgtagct
     8521 caaagtaaaa agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat
     8581 gaatttgctg gttgaaaaat caacaaaaac aacatgctga cagacaaaac cccggaatga
     8641 ccggcattat cggggcttag ggaagattta cttctgtaac caccagacag cctcaaaagg
     8701 ccgtaaattc atggcacagg gttgtggtga ggcttcttcg tagttatgca tcacaagctg
     8761 ccagttgccg cgcatttgcc ctggctgcca gggttggatc tcacggctaa ggttggcaat
     8821 gaccagcaag gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg
     8881 cagcagatcc tggtaattgc cccatgtcag gatggcttct tgcttacgca gtgcgattaa
     8941 cttttggtag gtgtaaaaca cagaggagtc atcggccagc gcggcttcta cgttgatttg
     9001 ttgatagtta tcgcctaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc
     9061 gttgctccat tgcatgggcg tgcggctgtt gtcacgggat ttactggcga ggattgccaa
     9121 taactcgtcg gcatcacgac catcgttgcg cagctcggca aacatattga ggctctccac
     9181 gtcgcgatag tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct cttcgccctg
     9241 gtagatatac ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg
     9301 cacgcggtat tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca
     9361 ccagaacaag gcattccatg ccacgttgtg cattccttgt tgccagtggc ggaacaatgt
     9421 tttcaacgcc acaaagtcag gtttagccag cgtccatttt tcaccaccgg gataatcgac
     9481 cttcaggtga tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg
     9541 ctggcaatgc tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt
     9601 aaacacatcg cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa
     9661 gcgacgcccg tcgccatcca ggtcttcagg gaaacgcggg tctttggaga tcagattcac
     9721 cacatccagg cgcaacccgt cgaccccacg atcggcccag aattcacaga cttttttcag
     9781 ctctgcgcgt accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg
     9841 caaatagtac tgttcgcttt ccgcatgcca gcgccacgca ctaccgccaa attttgaacg
     9901 ccagttgttc ggtggcgttt ctggttcgcc atcgcgccag atataaaact ggcggtaagg
     9961 gctttctttg ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac
    10021 catatcgaga atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc
    10081 gtccagcgtg ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt aaccgttatc
    10141 gacctgggga gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag
    10201 atagtccagg cgttggataa cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg
    10261 aaaactcttt ggataaatct ggtagataac gccgttttgc caccagtggg gaagattagt
    10321 cattacgtta ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa
    10381 tgtccagcgt gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga
    10441 tgatgatggc gatagccatt gccagcgcaa atacctgcca gtagctcggt tgaatagaga
    10501 gaattcccgg caggccccct acgccgatac cattcgccat aacgccgttc aggccgcata
    10561 gcaatcctgc cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt
    10621 tgatgccgta cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg
    10681 agatctcgcg ttcattgtgc ttgcggctgg aaatgataat gcctatcacg gcggagccct
    10741 gagcgatatt cgacagcgca atcagcggcc acactggcgt gccacccatg ctttgaatca
    10801 tctgcaaatc aatagcaagg gtggtctggt gtacgccggt gatcaccagc ggcgcgtaca
    10861 ggaagccaaa caatgccgca ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg
    10921 caaaggcaac gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga
    10981 acaccgcgag gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggtacga
    11041 tgcgtttaag gcgagtttca ataacgccca gcgccagccc ggctaacagt gccgggatca
    11101 cctgcgcctg atagcccact ttggcgatgc tgaacatgcc aaagtcccac acttccggca
    11161 gctgctgccc gagcagataa gcgttcatca gctgggggga aaccagtgtc acgccaagca
    11221 cgataccaag gatcggcgtg ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg
    11281 gcaggtagaa gaaaatcgct tcaccgatca accacagaaa atcgtagatc gtttgcaggg
    11341 aagggtacat ttgcgccagc gtctgaccgt tgctcatggg caaatcgccg atcacattgc
    11401 gaaaaccgag gatcaaaccg ccgctaatca acgcgggcag caacgggaag aagatctccg
    11461 cgaaatgaga gatcaactgc tcatgccatt tcatattttg ccgggcggct ttttttacct
    11521 gctctttatc aacctgcgcc tgtccggttg acgctattag tgcttgatag taatcaccca
    11581 cgttggtgcc aatcaccacc tgaaattgcc cggcattggt gaaacagcct ttcaccatgg
    11641 ggagttgctc aatttctttc ggtctggcat tggccggttg gttgaggaca aagcgcaggc
    11701 gagtaataca gtggctcacc gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc
    11761 gatcgatatc cgtttggttt attttgctca tcataaagcc ccatggcaga tgacattttt
    11821 ggttggctgc agaatatatt gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca
    11881 gcgaagatca caatttatcg ttcaggaaac gatcaggaca gggtggcggg gatgatgatt
    11941 tgttgcggtt cgctgcgccc ggttacctgt gcgatcaatt ggcaggccgc ctggcgtcca
    12001 gcttcggcgt agccaggatc tacggtgacg atctccggat gaaggaattt cattaacggc
    12061 gtattaccta cgctcgccag ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt
    12121 gcgccaagag ccagcgtgtc ggttgcgcac agtaacgcgg tagtttcagg cgtaatcact
    12181 tttgctacgt tttcatagcc ttgcttcata gcaagccctg gcagagcggc aacgggatgc
    12241 agtttatgcg ctttgcagaa cgccaggtag gcttcgtgac gtcgcttccc ggtcgtcacg
    12301 tcactatgcg gcacgccgag ataactgata ttgcgatgcc cctggtcata gagccgttgc
    12361 atcagaatct tgattgcccc ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca
    12421 cgcgccagca gaaccagcga tgactgccag tgggctaaca tttcttcggt tatgccggta
    12481 aaaccgaaca gcactacgcc gtcgatatta cgccgtttca gcacccccaa atgttcggca
    12541 actaattgtg gggaaaactg attttccatc atgattggat cgtaaccttg ttcatagaac
    12601 gctggcagca tggtttgaac ggcgagattt tctgacaacg aatccagacg ggtaacaatg
    12661 atggcgacca ctttatcgct ttgcccacgc atagcgcgcg cagagcggga aggggaaaat
    12721 ccatgctgat tcatcactgc ttcaacacgc tcgcgcgtgc gctgactcac gccgctttcg
    12781 ttattcagca cccgggaaac cgtagatttc cccacgccgc ttaagcgcgc gatgtctttg
    12841 atggtcagcc gattttgcat cctgttgtcc cataacgtgt tgtttaatta tttgagccta
    12901 actttacccg tgcattcagc aatgggtaaa gtctggttta tcgttggttt agttgccagc
    12961 aggtattata tcgccataaa tgctacgaat attattggat tctccttatt atttgcggcg
    13021 cttttttcac ttaccggagg ttatatggaa cctgatccca cgcctctccc tcgacggaga
    13081 ttaaaacttt tccggtaagc ccgtcttttc acggcgttac cggatgcgta aggccgtgac
    13141 gttttaatgt ccctgctcag ctttattacc ttcaggtaag gcttcgccac gcctgaagac
    13201 atttctgtac tgtttcagac agtgcggagg gactccttat gtttaaagaa atttttaccc
    13261 ggttcattcg ccatttacct tcccgtctgg ttcatcgcga tccattgcct ggcgcgcagc
    13321 agacagtgaa tacggcggtc ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga
    13381 tgcccgaaga agaattgtgg aaaacgttcg acacccatcc ggaaggatta aatcaggcgg
    13441 aagtggaatc tgcccgcgaa caacatggtg ataataaatt acccgcccaa caaccgtcgc
    13501 cgtggtgggt acatttatgg gtctgctatc gcaacccctt taatattttg ctcaccattc
    13561 ttggtgctat ttcttacgcc acggaagatt tatttgccgc aggtgttatc gcgctaatgg
    13621 tcgctatttc tacgttgctg aactttattc aggaagctcg ttccaccaaa gcggcagatg
    13681 ccctgaaagc gatggtcagt aataccgcga cggtactgcg agtaattaac gacaaaggcg
    13741 aaaatggctg gctggagatc ccgatagacc agctggtgcc cggcgatatt ataaaactgg
    13801 cggcggggga tatgatcccg gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg
    13861 ctcaggcgtc gttaaccggt gagtctctgc ccgtggaaaa agccgctact actcgccagc
    13921 cggagcacag caatccgctg gagtgcgaca ccctgtgttt tatgggcacc accgtggtga
    13981 gcggcacggc acaagcaatg gtgattgcta caggagccaa cacctggttt ggtcaactgg
    14041 cggggcgtgt tagtgagcag gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg
    14101 ttagtatgct gctgattcgc tttatgctgg tgatggctcc ggtggtgttg cttatcaacg
    14161 gttacaccaa aggcgactgg tgggaagcgg cactgtttgc gctttcggtc gcggtaggct
    14221 taacgccgga aatgttgccg atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc
    14281 tgtcgaaaca gaaagtgatc gtcaaacatc tggatgccat tcagaacttt ggcgcaatgg
    14341 atattctgtg cactgataaa accggcaccc tgacgcagga caaaattgtg ctggagaatc
    14401 ataccgatat ctccggtaaa accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc
    14461 attaccagac cggacttaaa aatctgcttg ataccgcggt gctcgaaggt acggatgaag
    14521 agtcagcgcg ttcgctggcc agccgttggc aaaaaattga tgagattccg tttgatttcg
    14581 agcgtcgtcg gatgtcggtg gtagtggcag aaaataccga acaccatcag ctggtttgca
    14641 aaggtgcatt gcaggaaatc ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg
    14701 taccgctcga tgacaccatg ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc
    14761 aggggctgcg cgtggttgcg gtggcgacga aatacctgcc agcgcgtgaa ggagattacc
    14821 agcgggcgga tgaatccgac ctgatcctcg aaggatatat tgctttcctt gatccgccaa
    14881 aagagacaac cgctccggca ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc
    14941 ttactggcga cagtgagtta gttgcagcga aagtgtgcca tgaagtgggg ctggatgcgg
    15001 gagaggtggt cattggtagt gatattgaaa cgctatctga cgatgaactg gcaaatctcg
    15061 cacagcgcac cacgctgttt gcccgcctga cgccgatgca taaagaacgc attgtgactt
    15121 tactgaagcg cgaagggcat gtggttggct ttatgggcga tggtattaat gatgcgcccg
    15181 ccttacgcgc tgcggatatc ggcatttctg tggacggcgc ggtagatatt gcccgtgaag
    15241 cggctgatat catcctgctg gaaaaaagcc tgatggtgct ggaagagggg gttattgagg
    15301 gacgtcgcac tttcgccaac atgctgaaat acatcaaatt gacggcgagt tctaacttcg
    15361 gtaatgtgtt cagcgtgctg gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt
    15421 tacacttgct tattcagaac ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata
    15481 acgtcgacga cgagcaaatt caaaagccgc agcgttggaa tccggcggat ctggggcgct
    15541 ttatggtctt cttcggaccg atcagctcga ttttcgatat tctgacgttt tgcttgatgt
    15601 ggtgggtatt ccatgccaac acgccggaaa cgcaaacgct gttccagtcg ggctggtttg
    15661 tggtgggatt actgtcgcaa acgctgattg tgcatatgat ccgtacccgc cgtgtgccgt
    15721 ttattcagag ctgtgcatcg tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg
    15781 ggatcgcatt gccgttttca ccgctggcca gttatctgca gctgcaggcg ctgccgttaa
    15841 gctatttccc gtggctggtt gcgattctgg cagggtatat gacgttaacc cagttggtga
    15901 aagggttcta tagccgtcgt tacggctggc agtaaagaat aaactgggca tggtggcccg
    15961 gattcagatt gcagacaaag tgagctttgt tcatgccgga tgcggcgtaa acgcccaatc
    16021 cggcctacaa attcaataaa ttgcaggaat cacgtaggcc tgataagcgt agcgcatcgg
    16081 gcgattttgc ttttgtcatc agacttaatc cgggcatgat agcccggatt tccatcaaga
    16141 ttagcgacga acagcgattg cttcgatctc aatcttcacg tctttcggca gacgggcaac
    16201 ttcaacgcaa gaacgtgccg ggaaagtggc gttgtgttcg gtgaagaagg cttcgtaagt
    16261 ggcgtttacg gttgcgaagt cgttcagatc tttcacgaac acagtggttt taacgatgtc
    16321 gcccactttc aggccagcgg cttcgacgat tgcttttaca ttatccagcg actgacgtgc
    16381 ctgtgcagcg acgtctgccg gtacttcacc agttttcgga tttaccggga tctgaccgga
    16441 ggtgatgatc atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc
    16501 attttccgtc gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg
    16561 gctcattata gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt
    16621 atgggaaaac tctttttcac agtatttgca tttaagcgcg atgtcattgg cgcgttttcg
    16681 cacggcaaag ctggatgaaa ccggttcggc atggctgata cagttgctgt tcgggcagac
    16741 cagcacattg tcgatgcgct ctggcagact tgggcgcgat ttacccacca cttcatagtt
    16801 gtcgatacgg ttaaccgtgg cttgcggtgc atacaatgcc agttggtcta cttgctcttc
    16861 actcaaaaag gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcaa
    16921 attcagacca atggtgatgc gctgatctgt ttcggtcaac ttgaacagac tcaacagctt
    16981 aaaaccgatc tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg
    17041 caatttatta tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac
    17101 cagtgccagt aacgcctggc gagcgaaaat cccgttgcct gcttgctgga agtaccaggc
    17161 gtgaggcgtt ttatccacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac
    17221 tttcatattg gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac
    17281 gttggcgtac tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc
    17341 tacttccgcc atcacttctt caatagagct gtgcagactc caggcgatcc ctttttcatc
    17401 gagcatatcc agaatgtatt gcggcattgc cagcgcatcc ggcgcgataa agtaaaaacg
    17461 gttgccgtcg aacttcgcta gcgcctgggt cagggagtga acggtgcggc catatttcag
    17521 gtcaccaacc attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatggtgaa
    17581 taagtccagc aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg
    17641 tacattgcca gaaaattcgg tggctaggcg tgccgcacct tcctgcggat gacgcatcac
    17701 tatcgcatcg acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcctttttt
    17761 acccagtgat gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat
    17821 ggaagtttcg aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac
    17881 tttgtgcttc aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac
    17941 cagattaagg tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg
    18001 attagccatc ttttatctcc tgacacctag gcaaaaaaaa gcccctcgat tgaggggctg
    18061 ggaatgggtg atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa
    18121 gacaaaatgt cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact
    18181 catagccaga agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt
    18241 gaattaaaag tcaaaatata tgcattttta ttttctatga ttctggaggg gaagtgtcgt
    18301 tcggtatcaa taattgcaga catttcctgc ttttttcttt ttcattttca cgcaatcaga
    18361 aatgcatttt attccattca tccgacttat agggcggagg gtgaaaacga acggctaaca
    18421 ctattgctta tagctcaggg atacgcgcta tcactttaat ttcaaaatca aagcctgcca
    18481 gccaggtaac acccaccgcc gtccagtttg gataaggtgg ggcgctaaat atttcatttt
    18541 tcaccgtcat gatgtcttca gattgttttt ccggatcggt atgaaagctc gtaacatcaa
    18601 tgatatcgtc aaaagtgcat cccgcagctg ccagggtcgc atgcaaatta tcaaaggcca
    18661 gtctgacttg ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc
    18721 cggaaacaaa cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca
    18781 gtgaatgtcg gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat
    18841 ttacgacatg aacagaagat ttatatttat cgggagccgc ttttaggggg acgacgttag
    18901 taaacaaaac ccagacatca tggataatgg ctgggcttaa ttgagcgtag tcggttatgc
    18961 gccaaacgcg ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc
    19021 taaccatgcg accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa
    19081 actatgcaac atatcgcgca ttggcccgtt ggcgggatta acgtcggtat caattggtcc
    19141 tggctggacg acgttgatgg tgatccctcg cggtccaaaa tcacgggcca gcccgcgcgc
    19201 catgccttgc agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg
    19261 atcgccattc acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc
    19321 ttcaacagag gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc
    19381 ggcatttaat tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc
    19441 caatgcgccg ctcttacgaa cgacatcaat aacagcgtct ctgtcagcac tatctgtgaa
    19501 tactgctgtc gctccagtct cttgtgccag acgttcagcg gcatctttcg accccgcata
    19561 ggtgaatcgt acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc
    19621 acgactgcca ccgaggatga gaactgtctt accggtaaaa gcgcccataa ggactccttg
    19681 atttattatg taacatgcat tacaaaactg ttttaacttt ctgtcaacag gttttgtaat
    19741 ggtcactaaa aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc
    19801 agtctctgcg gcaaaagtgc tttttcacca aaaaggtttc gatgcagtaa gtgtctctga
    19861 agttactgat tatcttggta ttaacccccc gagtctctac gcggcttttg gcagtaaggc
    19921 tgggttattt agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga
    19981 tattcttcgt gatgatcgtc cggtaggcga gtgcctggtt gaggtattaa aagaagcggc
    20041 gcgcagatat agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gtattcatag
    20101 tcatgatcca caagcgcgtg atattgccat tcaatattat cacgccgcag aaacgaccat
    20161 ttatgactat atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag
    20221 taccgtgatg tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg
    20281 tgcaacagct gccctggcag gggaagcgat aaaaactctt ctcaaggagt gatgctggcc
    20341 ttgatccgaa aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg
    20401 ttaaggctgg cggaattgat catcttttcg tataacgata gaaatgaagc attgttttaa
    20461 ttaaggagtg gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca
    20521 ggagttacgc caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg
    20581 caagcatgat atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc
    20641 gtacgaagct cgccgtgcgg agtaccatgc ccgctatctt gacattcaga ttgtgttaaa
    20701 aggtcaggaa ggcatgacct tcagcacgca acctgcaggc gcgccggata ccgactggtt
    20761 agctgataaa gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatcctaaa
    20821 tgaaggtgat tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg
    20881 cgcgccagcc caggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc
    20941 cgtaaataac tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg
    21001 tgagtaaaat ttgtaatgct tagacgttct tattaactca aggagttcgt catgggcaaa
    21061 atatcagatt tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaaat
    21121 gaagccttag atacctggta tgtgggaatg aatgattttg cccgaattgc cggggggcag
    21181 aatagcagaa gcaatattct ttctcctaga gcatttttgg agtttttggc aaagatattt
    21241 accctgggtt atgtggattt tagcaaacgc tccaacgaag cgggtagaaa tatgatggct
    21301 catattgagt tctcatctta ttctaaagat actgatggca atgaaaaaat gaagttttac
    21361 atgaataatc ctgaagggga gcgagcggat ttatctaagg tgaagataga aattacactt
    21421 gcctcagctt ctactaaagg gattcgtgaa ggacatacag tcattatatt taaacaatct
    21481 gatggttcga ctaatcgtta tgaagggaag tcttttgaaa gaaaagatga tagttcatta
    21541 cacctgatta ctaacaaggt tctggcgtgt taccaacgcg aagctaacaa gaaaatagcg
    21601 cgtctattaa ataatcatca gaagttaaat aatatacagg agttaaatga ttcgcaggag
    21661 ttaaataatt cacagaagtt aaataattcg caggagttaa ataactcgca ggtgagttgt
    21721 aaaggttcag ttgattctac gattacggat ttattagaaa aagcattgaa ta
//
LOCUS       DAZSNG010000062        21504 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZSNG010000062 DAZSNG010000000
VERSION     DAZSNG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.063"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHW4Z_002489"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHW4Z_002489"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR8882086.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHW4Z_002490"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHW4Z_002490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR8882087.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHW4Z_002491"
     CDS             complement(3121..3420)
                     /locus_tag="ACHW4Z_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR8882088.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHW4Z_002492"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHW4Z_002492"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR8882089.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHW4Z_002493"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHW4Z_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR8882090.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHW4Z_002494"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHW4Z_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR8882091.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHW4Z_002495"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHW4Z_002495"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR8882092.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHW4Z_002496"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHW4Z_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACHW4Z_002497"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACHW4Z_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8882093.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACHW4Z_002498"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACHW4Z_002498"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR8882094.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACHW4Z_002499"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACHW4Z_002499"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR8882095.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACHW4Z_002500"
                     /pseudo
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACHW4Z_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13973..14797)
                     /locus_tag="ACHW4Z_002501"
     CDS             complement(13973..14797)
                     /locus_tag="ACHW4Z_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR8882096.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACHW4Z_002502"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACHW4Z_002502"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8882097.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACHW4Z_002503"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACHW4Z_002503"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR8882098.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="ACHW4Z_002504"
     CDS             complement(18729..19202)
                     /locus_tag="ACHW4Z_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8882099.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHW4Z_002505"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHW4Z_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR8882100.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LVHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHW4Z_002506"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHW4Z_002506"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR8882101.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21504
                     /locus_tag="ACHW4Z_002507"
     CDS             21362..>21504
                     /locus_tag="ACHW4Z_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR8882102.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5405 a         5490 c         5275 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcaccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcaccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgca
//
LOCUS       DAZSNG010000063        21256 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZSNG010000063 DAZSNG010000000
VERSION     DAZSNG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.064"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..794
                     /locus_tag="ACHW4Z_002508"
                     /pseudo
     CDS             1..794
                     /locus_tag="ACHW4Z_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="ACHW4Z_002509"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="ACHW4Z_002509"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR8882103.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="ACHW4Z_002510"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="ACHW4Z_002510"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR8882104.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="ACHW4Z_002511"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="ACHW4Z_002511"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR8882105.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /gene="relA"
                     /locus_tag="ACHW4Z_002512"
     CDS             complement(4992..7226)
                     /gene="relA"
                     /locus_tag="ACHW4Z_002512"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR8882106.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="ACHW4Z_002513"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="ACHW4Z_002513"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR8882107.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="ACHW4Z_002514"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="ACHW4Z_002514"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR8882108.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="ACHW4Z_002515"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="ACHW4Z_002515"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR8882109.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="ACHW4Z_002516"
     CDS             complement(12981..14321)
                     /locus_tag="ACHW4Z_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR8882110.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="ACHW4Z_002517"
     CDS             complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="ACHW4Z_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR8882111.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="ACHW4Z_002518"
     CDS             complement(16109..16558)
                     /locus_tag="ACHW4Z_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR8882112.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="ACHW4Z_002519"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="ACHW4Z_002519"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR8882113.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="ACHW4Z_002520"
     CDS             complement(17358..17687)
                     /locus_tag="ACHW4Z_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR8882114.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="ACHW4Z_002521"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="ACHW4Z_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR8882115.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="ACHW4Z_002522"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="ACHW4Z_002522"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR8882116.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="ACHW4Z_002523"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="ACHW4Z_002523"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR8882117.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5068 g         5289 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcgtcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 ttcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgacaag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccatcat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctcc
//
LOCUS       DAZSNG010000064        20796 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZSNG010000064 DAZSNG010000000
VERSION     DAZSNG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20796
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.065"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHW4Z_002524"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHW4Z_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR8882118.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHW4Z_002525"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHW4Z_002525"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR8882119.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHW4Z_002526"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHW4Z_002526"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR8882120.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHW4Z_002527"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHW4Z_002527"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR8882121.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHW4Z_002528"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHW4Z_002528"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR8882122.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHW4Z_002529"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHW4Z_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR8882123.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACHW4Z_002530"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACHW4Z_002530"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR8882124.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACHW4Z_002531"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACHW4Z_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR8882125.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACHW4Z_002532"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACHW4Z_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR8882126.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACHW4Z_002533"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACHW4Z_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR8882127.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..11307)
                     /gene="rsxC"
                     /locus_tag="ACHW4Z_002534"
     CDS             complement(9181..11307)
                     /gene="rsxC"
                     /locus_tag="ACHW4Z_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR8882128.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11300..11878)
                     /gene="rsxB"
                     /locus_tag="ACHW4Z_002535"
     CDS             complement(11300..11878)
                     /gene="rsxB"
                     /locus_tag="ACHW4Z_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR8882129.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11878..12459)
                     /gene="rsxA"
                     /locus_tag="ACHW4Z_002536"
     CDS             complement(11878..12459)
                     /gene="rsxA"
                     /locus_tag="ACHW4Z_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR8882130.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12536..12976)
                     /locus_tag="ACHW4Z_002537"
     CDS             complement(12536..12976)
                     /locus_tag="ACHW4Z_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR8882131.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13062..13277)
                     /gene="ydgT"
                     /locus_tag="ACHW4Z_002538"
     CDS             complement(13062..13277)
                     /gene="ydgT"
                     /locus_tag="ACHW4Z_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR8882132.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13550..13675)
                     /gene="blr"
                     /locus_tag="ACHW4Z_002539"
     CDS             complement(13550..13675)
                     /gene="blr"
                     /locus_tag="ACHW4Z_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR8882133.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13918..14958
                     /locus_tag="ACHW4Z_002540"
     CDS             13918..14958
                     /locus_tag="ACHW4Z_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8882134.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14994..15995)
                     /gene="add"
                     /locus_tag="ACHW4Z_002541"
     CDS             complement(14994..15995)
                     /gene="add"
                     /locus_tag="ACHW4Z_002541"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR8882135.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16099..17271)
                     /gene="malY"
                     /locus_tag="ACHW4Z_002542"
     CDS             complement(16099..17271)
                     /gene="malY"
                     /locus_tag="ACHW4Z_002542"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR8882136.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17281..18873)
                     /gene="malX"
                     /locus_tag="ACHW4Z_002543"
     CDS             complement(17281..18873)
                     /gene="malX"
                     /locus_tag="ACHW4Z_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR8882137.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19048..20076
                     /gene="malI"
                     /locus_tag="ACHW4Z_002544"
     CDS             19048..20076
                     /gene="malI"
                     /locus_tag="ACHW4Z_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR8882138.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20188..>20796
                     /gene="hdhA"
                     /locus_tag="ACHW4Z_002545"
     CDS             20188..>20796
                     /gene="hdhA"
                     /locus_tag="ACHW4Z_002545"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR8882139.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5308 a         5235 c         5335 g         4918 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gcggcttcga cggcggcttt gcgcggatcg acctgttctt ctggttccgc
     9481 attagcctgt tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc
     9541 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
     9601 gcgcgctttg gagcgggcga tagctgcttc aacggcagtt ttacgtggtt cagcaacggt
     9661 tgctgcgtcg ttagtttgct ccagttctgc ctgtttcgct ctggcttgcg ctttacgggc
     9721 ttcccgttct gcaataattg cactgttatc cgggcgttcg cccgctttaa tcacaatagg
     9781 ctgtgtagcc tgggcctgtt tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt
     9841 atctttggct gcaggttgaa cggctgcgct cttatgtcgt tcaaggcgag tcgctttttc
     9901 gcgctccaga cgagcctggc gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc
     9961 ttcctgacga atagccgcaa tttcagcttt ttcctgacgg aaatattgca ccaagggaat
    10021 attgctcggg caaacccacg cgcaagcccc acattcaatg cagtcggcaa tgttatgcgt
    10081 ggtagcttta tcgtgttgct gacctttgct gaaccagtac aactgttgcg gcaaaagatc
    10141 cacagggcag gcgtcagcac aggcgctaca ccggatgcag ctttgttctt cctgtggttc
    10201 gccaagttca ttggcagagg gagccaacag acagttggta atctttacga ccgggacatc
    10261 cagccatggc aaggtaaagc ccattagcgg gccacccata atcaccattt gatcggcaga
    10321 ggggcagaat ccggcatcat tcaataaatg acgcactggc gtccccagcc gtgcccagac
    10381 gttgcccggg cgagcgattg cttcgccagt cagggttaca acacgctcgg taatcggctc
    10441 gccatcaata acggcacgtt tcactgcata agcagtgccg acgttttgca ttaatacgcc
    10501 gatatcggat gaacgcccgc catgtggaac ctgcttcccg gtcagaatgt aggttaattg
    10561 tttagcaccg ccagaaggat atttggttgg aatcacccgc agagaaatat cgttagagtc
    10621 cgccagcacc gcgcgcagca tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag
    10681 aatttcgcgt ggctgcagaa tatgcgcaag aatgcgaata ccctctacga cctgagccgc
    10741 gcaatcctgc atcaaacggt catcggcggt aatgtacggc tcgcactcag cagcgttgat
    10801 aatcaacgtt tcaatcttat ctccaccacc ctgcaattta acgcctgtcg ggaatccggc
    10861 accgcccagc ccggcaacac caaactgatg tatgcgctcg attaactctt cgcgactgcg
    10921 agagcgataa tcgacccagc cgtcgcgcgg gatccagcag tcttcaccat cggcatcaat
    10981 aatcacgctt aattcagcta aagctgaagg atgagccgta gagtggggcg caatagccgt
    11041 aacggtaccc gaggtgggcg cgtgaacagg cagcattttg ccgcgaccac gggtaagcgg
    11101 ctggccgcgc aatactttat cgccgacgct aacgcacaac tcaccttcag cgccaatatg
    11161 ctgtttcagt ggaataacaa aatgctgcgc cagtagtacc tggcgcaggg gggtaccgtt
    11221 ggtctgggtt ttcatctccg gtggatggat gccgccgttg aaatcccaga ttttattttt
    11281 tctgaatgca gagaataact taagcatggt gttccacggg aatgatacgc acgggaatgg
    11341 tgttcagatc ccatttccag gagtcaggtg tttctgcgac cggttgcaac gagatgcagt
    11401 gcgtcgggca cggatcaaca cataaattgc agcccgtaca gagatcactc attaccgtat
    11461 gcatgacacg ggtagcgcca acgatggcgt ctaccggaca agcctgaata catttagtgc
    11521 agccaataca gttattttca tcaataaccg ccaccatccg cgcaggcgtt agctcttgcg
    11581 cttcgccatc cagcggctgc ggctcgacat taagcaactc ggcaattttt agcatcacag
    11641 cttcgccacc tggggcgcaa cggttgattt tttcaccgtt acagctgatg gcttccgcgt
    11701 aggggcgaca gccgggataa ccgcactgac cacactggct ctgcggtaag atttcgtcaa
    11761 ttttctcaac gaccggatcg tcttccaccg caaaacggcg ggaggcataa cccagaatgg
    11821 cgccaaacgc caggcccagc aggctcacgg cggcaacggc aatccagata gcattcatta
    11881 caacttcacc aaaccactaa agcccataaa ggccagagac ataagacctg cggtaattaa
    11941 cgcaatggca ttaccgcgaa aaggtgccgg gacatcagcc acagcaaggc gttcgcggat
    12001 ggcggcgaag agcaccatca ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc
    12061 cgactgcaag aaattgtgcc cgagattgat attcagcaac gccacgccga gcactgcaca
    12121 gttggtggtg ataagcggca aaaaaatccc cagcagacgg taaagcaccg ggctggtttt
    12181 gcgcaccacc atctcggtga actgcacgac cacagcaatc accagaataa atgccagggt
    12241 gcgcaggtaa atcagattaa gtgggatcaa aatccacgta tcgataagcc aggcgcaaat
    12301 agacgtcagc gtcatcacaa acgttgttgc cagccccatg cccatcgcgg tttccagttt
    12361 tttggaaacc cccataaacg gacagagacc gagaaacttg accagtacaa agttattgac
    12421 cagtacagtt ccgacaaaca gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa
    12481 gccgcctatt atccgttaat ccagagcagg cgacaacagg ttaactgtaa ggttattacg
    12541 gattcacaaa cgtcgctttt acccgcgacg agcgtttgaa ataggggacg atcaatgcgg
    12601 ttgccaacaa agtaaacagc aactggcgaa cggctatgcc aacttccacg ggtgaaaaag
    12661 cgaatgcttt tactgccagc aatacggaaa taagcagcca gataatatag tgtttaggaa
    12721 cacaacgccg acgtttaaag aaagcgatgg tcaaccacag tgtgtaatac cacatggcga
    12781 tagcggtaat aaaagaaacg ccccataaga tttgtgtcgt cagtgcttgt ccgccgagcg
    12841 tttgaaatgt ttgcggagaa gataacgcag cggtatacag caacaacgcc agcgtcgtac
    12901 tcaacagagc gactaaaagc caggccaacg ggccaagcaa ccagcctcca atacgttgtg
    12961 gcgttgtggt ggtcataggt tctccagaat agcgacgcaa acaggcaaat ctaattttag
    13021 gggcgttatt ataaggcttt tataaccgat cgctactttc tttattggac atagtgccag
    13081 acggacttcg gtacctggcc gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga
    13141 cggtgatcgg cagcacgata catattgatc agttcctgat cgtccgtcag ggtgtaatta
    13201 agatggtcgt agagtttttc cagactttcg agtgaactga ttttgcgaaa ttttaataag
    13261 tagtcctgaa cagtcataaa aggtccataa aataattaat accaaagggg taggtattat
    13321 tagtgatgat aaaatttcac caaaagtgaa cgcttgtgtt attacaataa taagaatgcg
    13381 ctgatggcaa atattcaggg atgaaaaact gctctgaaaa gagtaacgcg ctttactatc
    13441 cctgacaatc accaacaaca tcgaacaaga taataaattc ctggtttaat atccgacaag
    13501 tgaaaacatg cacccggacg ggcagcatgt cgctccacaa gtgcagagct tacttgtgtt
    13561 gtaccgaagc actctgttca ggtggctgat agttgtcaat gtgactcgcc acgccaagaa
    13621 gaatgactga aacgacaaga acgatccaac ctgttaattc aataagacga ttcattacag
    13681 cccacatctc tcttgattga tccattaact tcagggggta aatgttactt agcaataata
    13741 gctccagcaa gatttttact gaggtttttg cgatatttag cttttgtcgt tggaaaattc
    13801 gctatttttt tgacttaagt taaacaacat cccttattgc tggcaggtta ttaaactgtt
    13861 gagcgtgggt aaaggatagt gtcaaatagc catcatactt caatgagagg caatgacatg
    13921 agcgacaaca tccgtgttgg gttgattggg tatggttatg cgagcaaaac cttccatgcg
    13981 cccctgattg cgggcacgcc cgggctggaa ctggcggtaa tctccagcag tgatgaaaca
    14041 aaagtaaaag ccgactggcc aacggttacg gttgtctctg agccgaagca tctgtttaac
    14101 gatcccaaca tagacctgat tgtcattcct acacccaacg atacccattt cccgttagcc
    14161 aaagcggcgc ttgaggcggg taaacatgtg gtcgttgata aaccctttac cgtgacactg
    14221 tcacaagcgc gagagctgga tgcgctggca aaaagcctgg ggcgtgtgct gtctgtattc
    14281 cataaccgcc gttgggatag cgatttcttg acgctaaaag gtttgctcgc ggaaggcgtg
    14341 ctgggtgaag ttgcttactt tgagtctcat tttgaccgct tccgtccgca ggtgcgcgat
    14401 cgttggcgtg aacagggcgg cccaggcagc ggtatctggt acgatttagc accgcatctt
    14461 cttgatcagg ccattacgct atttggttta ccggtcagca tgacggtaga tttggcacag
    14521 ttacggcccg gagcgcagtc gaccgattat ttccacgcca tcttgtccta tccacagcgg
    14581 cgagtcattt tacacggtac catgctggca gccgctgagt cagcacggta tatcgtgcat
    14641 ggatcccgag gcagttatgt gaaatatggc ctcgatccac aggaagaacg tctgaaaaat
    14701 ggcgagcgtc tgccgcagga agactggggc tacgatatgc gtgatggcgt acttacccgc
    14761 gtggaaggtg aggaacgtgt cgaagaaacg ctgttgacgg tgcctgggaa ttatccggct
    14821 tactatgcgg cgattcgtga tgcgttaaat ggcgatggtg aaaatccggt tccggcaagc
    14881 caggcaatcc aggtaatgga gttgattgag ctgggcatcg aatccgccaa acatcgcgcg
    14941 acgttgtgcc ttgcgtgaaa aatggatggt gcaatttgca ccatccattt ttgttacttc
    15001 gcggcgactt tttctcgcag tgcgcgtttt tcctctgcgc tgaggaaagc catttccaga
    15061 ccattaatct gtgcctggcg gatttgctca cgggataacc cagcagctgg cgcggcaacg
    15121 gtatattcgt gaatgatatc cactccctgt acgccgggat cgtcagtgtt aatgctggca
    15181 cgaatgccat gctcaaggaa cgttttcagc ggatgtgcag ccagctctgc tacggtgctg
    15241 gtctgaatat tggaggtcag acaggattca ataccaattt gttgctcggc gagaaaatcc
    15301 atcagcgccc gatcttcaat ggcttttacg ccatgtccaa tacgctccgc acccagttca
    15361 cgaatcgcct gccagatgct ttccggcccg gcagcttcgc ctgcatggac ggtaatatgc
    15421 cagcccgcat cacgcgcgcg gttgaagtga gaaaggaaca gacttcccgg gaatccaagt
    15481 tcatcaccag ctaaatcaag tgcggtaatc tggtcacggt gggctaaaaa ggcctccagc
    15541 tcttgctgac aggcggcttc gccgaaggtt cggctcataa tgccgataag cttcgcctgc
    15601 acaccaaagg tgcggcaacc ttcacgtacg ccatcgatca ccgcttcgac aacacccgct
    15661 acaggcagct tatgtgccat tgccatgtag cctggtgaaa aacgcagctc gacatagcgc
    15721 aggccgttac gggctgcatc ttcaatgttt tcaaatgcca cgcggcggca ggcatcaaga
    15781 gaggcgagaa ctttaacgcc ccagtcaagt ttggtcagaa agctcaccag atcgggttcg
    15841 ttggcaatga cctgaacgtg gggaatcagt gtttcaaggg attgtgcagg aagcgagata
    15901 ttatactggc ggccaagttc aagaatggtc tggggacgaa tgttgccatc aaggtggcga
    15961 tggatatcag ttaatggcag ggtggtatca atcatggtcg cactcttttt ctggatataa
    16021 aagtgcgcac cattataaaa acaaagcagg gtaaaaatcc acgattgcgc aacgattttt
    16081 tacgttgcgc aattggggtt agcgaacagc gcggatggcg tttattaatc cagccacacc
    16141 tttttccagt ttcgaacgtg ggcagccggc attgagacgg acaaaaccac gaccttcttc
    16201 accataggta taccccggca tgatcgcgac tttttcttgc tcgataagcg ctttttgcaa
    16261 cgcgttgtcg tcaatattca acggacgtag atcaagccag gccagataag tggattgcgg
    16321 gatctgccag ttgagttcag gaaacgcggc gttcatttta tctgagatat acgtcaggtt
    16381 atctttcaga tagacgcgta aggcatccag ccacggtgca ccttgctgat aggcggcgat
    16441 atgggcagtt aacgccagta ccgaagggga agaaagccca tcacgtcctt tcagtgccga
    16501 taaataggca tcacggctgc tgctattttc tataatcccg taagcaccgg tcagcgcggg
    16561 aatattgaaa cttttcgatc ccgatgtcag caacgcccag tctccccgcg ccacattact
    16621 ccagggaata tgcggctgct cgccccagac catatccata tggatttcat cggaaataac
    16681 ccgcacatca tgacgctcgc acaggtcagc catgatctcc agctcatcgc acgtccacac
    16741 tttcccggta ggattctgtg ggttacacag gagcataatt ttacattctg gtttcgccaa
    16801 aacggcttcc agcttgccca tatcgcaaaa ccagccgtca gcctgcttct ctaaagcaac
    16861 gggcattact gtgcgctggt taccttcaat ggccttgtaa aatgcgtcat aggcgggcgt
    16921 gtggatcacc acgccttcac ctgtttcaga ccactgacga atcagttctg aaaccatata
    16981 gatgacagaa gggccataca ccaccgtctg cgtatcaatt gcggtgtaat gctgggtgga
    17041 aaaccagtgg gcaatagccg cgagaaactc atcgtttttc cagcggctgt agccaaatac
    17101 gccgtgcatc aggcgctgat tcagcgcctc gataatgcag ggggcagtgg caaaatccat
    17161 gtctgaaatc gtgaacggta acaggtcagc agtgccgaaa cggtcagcga cataatccca
    17221 ctgtgtacac catgtgccat gacgatccac gacctttgaa aaatcgaaca tatcttatcc
    17281 ttatgcctgg acagtatgca tcagaccggc catttcatct tttactgact gaacttgtgg
    17341 cccgataaca acctgcaggt tatgttgatt aagttgtact acgccaattg cccgattgtc
    17401 cttcagtgcc tgcacattaa caagcgacat atcttccaca gacaaacgca gacgggtaat
    17461 gcagttatcg aggctgacaa tattgtcggc accgcctaat gcttcgagga ttgcaggaac
    17521 gttgtaacct gatttacccg gcgcaccggc aacggctttt tcgattgagc tggcaacttc
    17581 gctatcgcgc cccggggttt tcagattgaa gcgggtgata gcgaaacgga agatgacgta
    17641 gtaaacgaca aaccagattg ccgccaccac tggcaccatg taccacttgg ttgacagacc
    17701 atgcaaaata ccgaacacca cgaagtcgat gatattgccg tcggtattac cgatggtgac
    17761 gccgagcaca gacatgacgg tgaagccgag gccggttaac agcgcgtgga tgacatacag
    17821 aactggcgct acgaacagga acaggaattc cagcggttcg gtagtgccgc caacgacgca
    17881 ggcgatcagg ccagaaatca gcagaccttt aattttatgg cgattttccg ggcgcgcgca
    17941 gtgatacata gctaacgctg cacctggtag accgccgaga aacgcaggca ttttaccttg
    18001 cgaaaggaaa cgcgtgacgc tttcagaaaa accgtgagtg gtcgggcaac tcaattgcgc
    18061 ctggaagatg gtcagtgcgc cgctgacggt ttgaccgcag acctcctgcg tgccgcctgc
    18121 gtcggtaaag cgaattaatg ccaccagaat gtgatgcaga ccaaacggca acagcagacg
    18181 ttcaccggta ccaaacagca tcggtccgaa atcacccgcg ctgtttatca tatggcccaa
    18241 gccgctaata cccatggcga aaatcggcca gactaatgga atcaccaggc cgacaaggcc
    18301 catcaccagc gaggagataa ttggtacgaa gcgcgtaccg ccgaagaatg ccagcgcatc
    18361 cggcaggcgg atattatgga aacgctcatg cagcatccag acgataatac cggcgatcac
    18421 cgcaccgagg atcccggtat cgatcgactg gatcccaaga atgctctgga tgttattggc
    18481 tttcagaacg gcggcatctg tggttggcag aatgccttta ttggtcaacc agaagtttac
    18541 cgcgaggttc attaccgcat aaccgacgaa gccagcgaat gccgctacgc ctttattttc
    18601 gcgtgccagg cccagcggga tggcgataca gaacatgaca ggcaggaaac taaaagcaaa
    18661 cgagccaatc ttactcatcc aggtaaagat agcctgcaac acggggttgc ccaggaccgg
    18721 gatcagggtt atgacatcat ggctgctaag agaactacca atgccgagca taatgccgca
    18781 gaacgacaat aatgccacgg gtaacatgaa ggttttgcct aactgctgga agaactccca
    18841 cagcgtgact ttcggtgctg ttttcgccgt cataaaacga ctcctcgtag agaacaatct
    18901 ggattcagta aattgcgcga gaagataaaa cgttttatca aattttagtg agtcataaat
    18961 cacattacgc aacgataata gcgggtataa gataaataaa aggtaaaacg ttttatctgt
    19021 cacataatca gggagtaggt catctgcatg gctaccgcca aaaaaataac cattcatgat
    19081 gttgcgctgg ctgcgggcgt gtcggtaagt accgtttcgc tggtgcttaa tggcaaaggg
    19141 cgaatctcta ccgccacagg agaacgcgtt aacgccgcca ttgaagagct gggatttgtg
    19201 cgcaatcgcc aggcgtcggc gctgcgcggc gggcaaagcg gcgtcattgg tttgatcgtc
    19261 cgtgatttat ctgcgccgtt ttacgccgaa ttgacggccg gattgacgga agctctggaa
    19321 gcacagggac ggatggtttt tttgcttcac ggcggtaaag acggcgagca gctggcacag
    19381 cggttttcac tgttactgaa tcagggtgtc gatggtgtgg taattgccgg ggctgcagga
    19441 agcagcgatg acctgcggcg gatggcagaa gaaaaagcta tcccggtgat tttcgcttcc
    19501 cgtgccagtt atcttgatga tgttgatacg gttcgcccgg acaacatgca ggctgcacag
    19561 ttgttgacgg agcatctcat tcgcaatggg catcagcgga tcgcctggct gggagggcaa
    19621 agttcctcat taacccgtgc agaacgggtg gggggctatt gtgcaactct actaaaattt
    19681 ggcctgccgt ttcacagcga ttgggtgttg gagtgcactt ccagccagaa gcaagccgcg
    19741 gaagctatca cggcgctttt acgtcataac ccgaccatca gtgccgtggt ttgctataac
    19801 gaaactattg cgatgggggc atggtttggt ttgctgaaag cagggcggca aagcggggaa
    19861 agcggagtcg atcgttactt tgagcaacag gtttcgctgg cggcatttac cgatgcgaca
    19921 ccaaccacac ttgatgatat acctgttacc tgggccagca cgccagcgcg ggaacttggt
    19981 atcacacttg cggatcgcat gatgcaaaaa atcacccatg aagagacgca ttcacgcaat
    20041 cttattattc ccgcccggct cattgcggcg aaataatcct ctctttatct gctatacttg
    20101 gtagtgtcct ttcctcaagg ttaatgataa aaaatgtggc acacaggcgg tgccttttat
    20161 ttttactgaa gttcacagga ggtttatgtg tttaattctg acaacctgag actcgacgga
    20221 aaatgcgcca tcatcacagg tgcgggtgca ggtattggta aagaaatcgc cattacattc
    20281 gcgacagctg gcgcatctgt ggtggtcagt gatattaacg ccgacgcagc taaccatgtt
    20341 gtagacgaaa ttcaacaact ggatggtcag gcatttgcct ggcgttgtga tattacttcc
    20401 gaacaggaac tctctgcact ggcagacttt gccgtcagta atctgggtaa agttgatatc
    20461 ctggttaaca acgccggtgg cggtggtcct aaaccgtttg atatgccaat ggcagatttt
    20521 cgccgtgctt atgaactgaa tgtgttttct tttttccatc tgtcacaact tgttgcgcca
    20581 gaaatggaaa aaaatggagg tggcgttatt ctgaccatca cttctatggc ggcagaaaat
    20641 aaaaatataa acatgacttc ctatgcatca tctaaagctg cggccagtca tctggtcaga
    20701 aatatggcat ttgacctggg tgaaaaaaat attcgggtga atagcattgc gccgggggca
    20761 atattaaccg atgccctgaa atccgttatt acacca
//
LOCUS       DAZSNG010000065        20780 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZSNG010000065 DAZSNG010000000
VERSION     DAZSNG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.066"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..579
                     /locus_tag="ACHW4Z_002546"
     CDS             <1..579
                     /locus_tag="ACHW4Z_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8882140.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHW4Z_002547"
     CDS             597..1235
                     /locus_tag="ACHW4Z_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8882141.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHW4Z_002548"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHW4Z_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR8882142.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHW4Z_002549"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHW4Z_002549"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR8882143.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHW4Z_002550"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHW4Z_002550"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR8882144.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHW4Z_002551"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHW4Z_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR8882145.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHW4Z_002552"
     CDS             complement(5552..6613)
                     /locus_tag="ACHW4Z_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR8882146.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHW4Z_002553"
     CDS             complement(6610..8007)
                     /locus_tag="ACHW4Z_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR8882147.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHW4Z_002554"
     CDS             8163..9161
                     /locus_tag="ACHW4Z_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8882148.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHW4Z_002555"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHW4Z_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHW4Z_002556"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHW4Z_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHW4Z_002557"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHW4Z_002557"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR8882149.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHW4Z_002558"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHW4Z_002558"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR8882150.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHW4Z_002559"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHW4Z_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHW4Z_002560"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHW4Z_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHW4Z_002561"
     CDS             complement(16089..17141)
                     /locus_tag="ACHW4Z_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR8882151.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHW4Z_002562"
     CDS             complement(17172..18014)
                     /locus_tag="ACHW4Z_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8882152.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHW4Z_002563"
     CDS             complement(18001..18882)
                     /locus_tag="ACHW4Z_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8882153.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHW4Z_002564"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHW4Z_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHW4Z_002565"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHW4Z_002565"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR8882154.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5073 a         5667 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gacgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctagtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAZSNG010000066        20713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZSNG010000066 DAZSNG010000000
VERSION     DAZSNG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.067"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="ACHW4Z_002566"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="ACHW4Z_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR8882155.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="ACHW4Z_002567"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="ACHW4Z_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR8882156.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="ACHW4Z_002568"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="ACHW4Z_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR8882157.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="ACHW4Z_002569"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="ACHW4Z_002569"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR8882158.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="ACHW4Z_002570"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="ACHW4Z_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR8882159.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="ACHW4Z_002571"
     CDS             complement(8218..9111)
                     /locus_tag="ACHW4Z_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR8882160.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="ACHW4Z_002572"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="ACHW4Z_002572"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR8882161.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="ACHW4Z_002573"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="ACHW4Z_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR8882162.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /gene="ycaQ"
                     /locus_tag="ACHW4Z_002574"
     CDS             complement(10242..11474)
                     /gene="ycaQ"
                     /locus_tag="ACHW4Z_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR8882163.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="ACHW4Z_002575"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="ACHW4Z_002575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR8882164.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="ACHW4Z_002576"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="ACHW4Z_002576"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR8882165.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="ACHW4Z_002577"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="ACHW4Z_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="ACHW4Z_002578"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="ACHW4Z_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR8882166.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="ACHW4Z_002579"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="ACHW4Z_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR8882167.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="ACHW4Z_002580"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="ACHW4Z_002580"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR8882168.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="ACHW4Z_002581"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="ACHW4Z_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR8882169.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5728 c         5031 g         4973 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatcctc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAZSNG010000067        20691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZSNG010000067 DAZSNG010000000
VERSION     DAZSNG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.068"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            81..350
                     /locus_tag="ACHW4Z_002582"
     CDS             81..350
                     /locus_tag="ACHW4Z_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR8882170.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHW4Z_002583"
     CDS             344..709
                     /locus_tag="ACHW4Z_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR8882171.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHW4Z_002584"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHW4Z_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR8882172.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHW4Z_002585"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHW4Z_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR8882173.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHW4Z_002586"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHW4Z_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR8882174.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHW4Z_002587"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHW4Z_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8882175.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHW4Z_002588"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHW4Z_002588"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR8882176.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHW4Z_002589"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHW4Z_002589"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR8882177.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHW4Z_002590"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHW4Z_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHW4Z_002591"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHW4Z_002591"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR8882178.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHW4Z_002592"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHW4Z_002592"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR8882179.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHW4Z_002593"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHW4Z_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR8882180.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHW4Z_002594"
     CDS             complement(16429..17187)
                     /locus_tag="ACHW4Z_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR8882181.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHW4Z_002595"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHW4Z_002595"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR8882182.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHW4Z_002596"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHW4Z_002596"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR8882183.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHW4Z_002597"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHW4Z_002597"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR8882184.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHW4Z_002598"
     CDS             complement(20306..20587)
                     /locus_tag="ACHW4Z_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882185.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4890 a         5693 c         5216 g         4892 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgcca atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZSNG010000068        20613 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZSNG010000068 DAZSNG010000000
VERSION     DAZSNG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20613
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.069"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            97..396
                     /locus_tag="ACHW4Z_002599"
     CDS             97..396
                     /locus_tag="ACHW4Z_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR8882186.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="ACHW4Z_002600"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="ACHW4Z_002600"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR8882187.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="ACHW4Z_002601"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="ACHW4Z_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR8882188.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="ACHW4Z_002602"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="ACHW4Z_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR8882189.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="ACHW4Z_002603"
     CDS             complement(2535..2879)
                     /locus_tag="ACHW4Z_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR8882190.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3360
                     /locus_tag="ACHW4Z_002604"
                     /pseudo
     CDS             3031..3360
                     /locus_tag="ACHW4Z_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="ACHW4Z_002605"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="ACHW4Z_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR8882191.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="ACHW4Z_002606"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="ACHW4Z_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR8882192.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="ACHW4Z_002607"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="ACHW4Z_002607"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8882193.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="ACHW4Z_002608"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="ACHW4Z_002608"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8882194.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="ACHW4Z_002609"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="ACHW4Z_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR8882195.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="ACHW4Z_002610"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="ACHW4Z_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR8882196.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="ACHW4Z_002611"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="ACHW4Z_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR8882197.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="ACHW4Z_002612"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="ACHW4Z_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /gene="ygaZ"
                     /locus_tag="ACHW4Z_002613"
     CDS             12505..13242
                     /gene="ygaZ"
                     /locus_tag="ACHW4Z_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR8882198.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="ACHW4Z_002614"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="ACHW4Z_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR8882199.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="ACHW4Z_002615"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="ACHW4Z_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR8882200.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="ACHW4Z_002616"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="ACHW4Z_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="ACHW4Z_002617"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="ACHW4Z_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR8882201.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="ACHW4Z_002618"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="ACHW4Z_002618"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR8882202.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="ACHW4Z_002619"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="ACHW4Z_002619"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR8882203.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19828)
                     /locus_tag="ACHW4Z_002620"
     CDS             complement(19400..19828)
                     /locus_tag="ACHW4Z_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR8882204.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="ACHW4Z_002621"
     CDS             complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="ACHW4Z_002621"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR8882205.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4974 a         5274 c         5148 g         5217 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcatga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatattg gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaagaaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    19621 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    19681 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    19741 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    19801 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    19861 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg
    19921 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    19981 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    20041 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact
    20101 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    20161 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    20221 tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta
    20281 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc
    20341 caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    20401 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    20461 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    20521 gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    20581 tagccgagta ctctatccag ctgagctacg gat
//
LOCUS       DAZSNG010000069        20521 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZSNG010000069 DAZSNG010000000
VERSION     DAZSNG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.070"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACHW4Z_002622"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACHW4Z_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR8882206.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACHW4Z_002623"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACHW4Z_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR8882207.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACHW4Z_002624"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACHW4Z_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR8882208.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHW4Z_002625"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHW4Z_002625"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR8882209.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHW4Z_002626"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHW4Z_002626"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR8882210.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACHW4Z_002627"
     CDS             4449..4814
                     /locus_tag="ACHW4Z_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR8882211.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACHW4Z_002628"
     CDS             4964..5482
                     /locus_tag="ACHW4Z_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR8882212.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACHW4Z_002629"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACHW4Z_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACHW4Z_002630"
     CDS             6713..7195
                     /locus_tag="ACHW4Z_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR8882213.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACHW4Z_002631"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACHW4Z_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR8882214.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACHW4Z_002632"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACHW4Z_002632"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR8882215.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACHW4Z_002633"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACHW4Z_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR8882216.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACHW4Z_002634"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACHW4Z_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR8882217.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACHW4Z_002635"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACHW4Z_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR8882218.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="ACHW4Z_002636"
     CDS             10939..11730
                     /locus_tag="ACHW4Z_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR8882219.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="ACHW4Z_002637"
     CDS             11751..13037
                     /locus_tag="ACHW4Z_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR8882220.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACHW4Z_002638"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACHW4Z_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR8882221.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="ACHW4Z_002639"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="ACHW4Z_002639"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR8882222.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="ACHW4Z_002640"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="ACHW4Z_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR8882223.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="ACHW4Z_002641"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="ACHW4Z_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR8882224.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="ACHW4Z_002642"
     CDS             complement(16985..17275)
                     /locus_tag="ACHW4Z_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR8882225.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACHW4Z_002643"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACHW4Z_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR8882226.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="ACHW4Z_002644"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="ACHW4Z_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR8882227.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="ACHW4Z_002645"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="ACHW4Z_002645"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="ACHW4Z_002646"
     CDS             19137..20378
                     /locus_tag="ACHW4Z_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8882228.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4814 g         5050 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atgtttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAZSNG010000070        20293 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZSNG010000070 DAZSNG010000000
VERSION     DAZSNG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20293
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.071"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHW4Z_002647"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHW4Z_002647"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR8882229.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHW4Z_002648"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHW4Z_002648"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR8882230.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3001)
                     /locus_tag="ACHW4Z_002649"
                     /pseudo
     CDS             complement(1729..3001)
                     /locus_tag="ACHW4Z_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3184..4389)
                     /locus_tag="ACHW4Z_002650"
     CDS             complement(3184..4389)
                     /locus_tag="ACHW4Z_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882231.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4489..4845)
                     /locus_tag="ACHW4Z_002651"
     CDS             complement(4489..4845)
                     /locus_tag="ACHW4Z_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR8882232.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4981..5088)
                     /gene="rprA"
                     /locus_tag="ACHW4Z_002652"
     ncRNA           complement(4981..5088)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHW4Z_002652"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACHW4Z_002653"
     CDS             complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACHW4Z_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR8882233.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6775..9831
                     /locus_tag="ACHW4Z_002654"
     CDS             6775..9831
                     /locus_tag="ACHW4Z_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR8882234.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9828..10238
                     /gene="menI"
                     /locus_tag="ACHW4Z_002655"
     CDS             9828..10238
                     /gene="menI"
                     /locus_tag="ACHW4Z_002655"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR8882235.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10338..10526
                     /locus_tag="ACHW4Z_002656"
     CDS             10338..10526
                     /locus_tag="ACHW4Z_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR8882236.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11074..11442
                     /gene="sufA"
                     /locus_tag="ACHW4Z_002657"
     CDS             11074..11442
                     /gene="sufA"
                     /locus_tag="ACHW4Z_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR8882237.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11451..12938
                     /gene="sufB"
                     /locus_tag="ACHW4Z_002658"
     CDS             11451..12938
                     /gene="sufB"
                     /locus_tag="ACHW4Z_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR8882238.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12948..13694
                     /gene="sufC"
                     /locus_tag="ACHW4Z_002659"
     CDS             12948..13694
                     /gene="sufC"
                     /locus_tag="ACHW4Z_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR8882239.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13669..14940
                     /gene="sufD"
                     /locus_tag="ACHW4Z_002660"
     CDS             13669..14940
                     /gene="sufD"
                     /locus_tag="ACHW4Z_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR8882240.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14937..16157
                     /gene="sufS"
                     /locus_tag="ACHW4Z_002661"
     CDS             14937..16157
                     /gene="sufS"
                     /locus_tag="ACHW4Z_002661"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR8882241.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16170..16586
                     /gene="sufE"
                     /locus_tag="ACHW4Z_002662"
     CDS             16170..16586
                     /gene="sufE"
                     /locus_tag="ACHW4Z_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR8882242.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16735..17739
                     /gene="ldtE"
                     /locus_tag="ACHW4Z_002663"
     CDS             16735..17739
                     /gene="ldtE"
                     /locus_tag="ACHW4Z_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR8882243.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACHW4Z_002664"
     CDS             complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACHW4Z_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR8882244.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACHW4Z_002665"
     CDS             complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACHW4Z_002665"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR8882245.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19769..19963)
                     /locus_tag="ACHW4Z_002666"
     CDS             complement(19769..19963)
                     /locus_tag="ACHW4Z_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882246.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5318 a         4862 c         5178 g         4935 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag
     2401 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2461 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2521 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2581 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2641 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2701 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2761 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2821 aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca
     2881 gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt
     2941 aaatacaaag aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta
     3001 ttgttctccg acggtttaca taattactat actgaagatg attttccctg ggtacatggc
     3061 ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc
     3121 ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt
     3181 gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct
     3241 gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc
     3301 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
     3361 gcccgtactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
     3421 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
     3481 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
     3541 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
     3601 aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
     3661 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
     3721 cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg
     3781 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
     3841 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
     3901 ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa
     3961 ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa
     4021 agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
     4081 aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc
     4141 catatagcag cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa
     4201 tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac
     4261 acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat
     4321 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg
     4381 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt
     4441 atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
     4501 ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa
     4561 attgccatca gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt
     4621 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg
     4681 acgcatagcc aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca
     4741 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
     4801 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
     4861 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
     4921 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
     4981 ttaaaaaaaa gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca
     5041 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
     5101 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
     5161 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
     5221 aatatcaatt agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta
     5281 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc
     5341 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
     5401 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg
     5461 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
     5521 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag
     5581 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
     5641 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
     5701 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
     5761 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
     5821 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
     5881 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
     5941 gtggtgccaa tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag
     6001 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
     6061 tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
     6121 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
     6181 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
     6241 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
     6301 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
     6361 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
     6421 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
     6481 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
     6541 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
     6601 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
     6661 ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc
     6721 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
     6781 ccacagattt ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     6841 gagcaacaag gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg
     6901 accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     6961 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
     7021 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     7081 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     7141 gaggccgggg tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt
     7201 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
     7261 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     7321 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg
     7381 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     7441 cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct taccggttac
     7501 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     7561 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct
     7621 aaagtgcgcc gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg
     7681 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
     7741 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac
     7801 aaagagatgc tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac
     7861 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt
     7921 gtgattggct ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc
     7981 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
     8041 gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg
     8101 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     8161 gtccgtccgg cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     8221 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
     8281 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
     8341 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
     8401 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
     8461 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
     8521 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
     8581 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg
     8641 gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
     8701 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
     8761 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag
     8821 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
     8881 cgttatttac gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg
     8941 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
     9001 tcggaaaaac atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa
     9061 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     9121 gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg
     9181 caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg
     9241 ccattttcgc caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag
     9301 acggcgaaaa agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg
     9361 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
     9421 gaacgtggcg agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca
     9481 catccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt
     9541 accgctttgc cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
     9601 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
     9661 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
     9721 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
     9781 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     9841 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
     9901 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     9961 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    10021 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    10081 tcaatgctaa tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt
    10141 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    10201 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    10261 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    10321 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    10381 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    10441 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    10501 tcgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    10561 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    10621 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    10681 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    10741 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    10801 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    10861 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    10921 gtgcctgtta acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt
    10981 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    11041 tatacgctga aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac
    11101 ccacaggatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc
    11161 gagctggtgg caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc
    11221 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    11281 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc
    11341 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    11401 gcccagaatg aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta
    11461 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag
    11521 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    11581 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    11641 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    11701 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    11761 cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    11821 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    11881 ccattttcga ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa
    11941 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    12001 tcggcaccgt ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct
    12061 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    12121 attttcgcat taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg
    12181 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    12241 tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    12301 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    12361 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    12421 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    12481 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    12541 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    12601 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    12661 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    12721 gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    12781 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    12841 acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa
    12901 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    12961 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    13021 gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    13081 caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    13141 aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc
    13201 agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    13261 cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    13321 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    13381 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    13441 agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg
    13501 ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc
    13561 aacgcattct cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg
    13621 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    13681 ccgaacagca gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa
    13741 cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    13801 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc
    13861 attgcgggag atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg
    13921 cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc
    13981 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa
    14041 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    14101 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    14161 gaggtgaaca ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    14221 gtgatcgaac attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact
    14281 atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc
    14341 agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat
    14401 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    14461 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    14521 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    14581 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    14641 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    14701 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    14761 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    14821 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    14881 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    14941 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    15001 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg
    15061 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    15121 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    15181 cccgttcggc ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg
    15241 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    15301 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    15361 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    15421 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc
    15481 cactggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    15541 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt
    15601 tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    15661 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    15721 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    15781 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca
    15841 tagccgagta tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg
    15901 atctcactct ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    15961 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    16021 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    16081 tggccatgta taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc
    16141 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    16201 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg
    16261 gccagcgtct gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct
    16321 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    16381 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    16441 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    16501 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    16561 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    16621 ttgccggatg gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca
    16681 taagcaccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    16741 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    16801 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    16861 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca
    16921 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata
    16981 actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt
    17041 gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata
    17101 ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc
    17161 cctaacccaa cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt
    17221 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    17281 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    17341 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    17401 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    17461 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    17521 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    17581 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    17641 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    17701 gaaccagagc aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa
    17761 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    17821 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    17881 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    17941 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    18001 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    18061 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    18121 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    18181 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaaa taaagtgaga
    18241 ttatatagtg aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa
    18301 gcgcccatca gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg
    18361 aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac
    18421 gtcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc
    18481 atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg
    18541 atgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg
    18601 tactgcgcga gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt
    18661 ttcagcagtt tcaacggcac cacggcatac cgcttcggtg atgcgcagtt tacggttgtc
    18721 attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat
    18781 gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc
    18841 gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg
    18901 gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat
    18961 acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc
    19021 caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt
    19081 gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt
    19141 caggtgctca cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa
    19201 gtctacgcct ttttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg
    19261 cagagcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt
    19321 cagcacttta cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc
    19381 atcgtcaacc agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac
    19441 cgcaaccatt tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc
    19501 agctttcaga gaaacatcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt
    19561 ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg
    19621 aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat
    19681 gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca
    19741 aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac
    19801 ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg
    19861 ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc
    19921 aacgtgagtg tctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca
    19981 agagtcaatt ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaacagcgt
    20041 ttcaaaaaat atacaacttg ttagacaata aattgttatc aaagatttaa cgttccaggg
    20101 aaaaggttac gcaaaaactt cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg
    20161 aggataagtg aaagttcatc cggtgtataa atatatcatc gctcaattgc tacagttaaa
    20221 ataatttatc gcgttatttg tctgattttg tttgattttt gtttaattgg ttagattgat
    20281 atatgctgat agt
//
LOCUS       DAZSNG010000071        19948 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZSNG010000071 DAZSNG010000000
VERSION     DAZSNG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19948
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.072"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..122
                     /locus_tag="ACHW4Z_002667"
     CDS             <1..122
                     /locus_tag="ACHW4Z_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR8882247.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHW4Z_002668"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHW4Z_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR8882248.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHW4Z_002669"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHW4Z_002669"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR8882249.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHW4Z_002670"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHW4Z_002670"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR8882250.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHW4Z_002671"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHW4Z_002671"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR8882251.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHW4Z_002672"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHW4Z_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR8882252.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHW4Z_002673"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHW4Z_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR8882253.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHW4Z_002674"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHW4Z_002674"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR8882254.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHW4Z_002675"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHW4Z_002675"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR8882255.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHW4Z_002676"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHW4Z_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR8882256.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHW4Z_002677"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHW4Z_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR8882257.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHW4Z_002678"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHW4Z_002678"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR8882258.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHW4Z_002679"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHW4Z_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR8882259.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHW4Z_002680"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHW4Z_002680"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR8882260.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHW4Z_002681"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHW4Z_002681"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8882261.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHW4Z_002682"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHW4Z_002682"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR8882262.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHW4Z_002683"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHW4Z_002683"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8882263.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..19428
                     /gene="rfbC"
                     /locus_tag="ACHW4Z_002684"
     CDS             18883..19428
                     /gene="rfbC"
                     /locus_tag="ACHW4Z_002684"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR8882264.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            19444..>19948
                     /gene="wzx"
                     /locus_tag="ACHW4Z_002685"
     CDS             19444..>19948
                     /gene="wzx"
                     /locus_tag="ACHW4Z_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR8882265.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAI"
BASE COUNT         4990 a         4773 c         5388 g         4797 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atggtcaaaa atttgtagat gctgattatt ttatatgata aagaaatgta
    19441 ataatgagca taataaaaaa tagtgtctgg aacctttttg gctatgcaat accaacttta
    19501 attgctatcc cctcgctagg atttctcgct cgaggtttgg ggcctgaagg tttcggtgtt
    19561 tatacaattg caattgcact tgttgggtat gctggaattt ttgatgtagg cctgactcgc
    19621 tctgttatta gagaaattgc aattcatcgt gataatcatc atgaaagaac caaggtaatt
    19681 tcaacaagta catctttttt ggtgctattt tcatgctttg gtgctttttt attattgatt
    19741 ttctctgatg gaattgttaa ttatctaaaa atttctggtg ttgaacatag tgatatacaa
    19801 ctagcattta aactgttggc tatttgcatt ccattattta ttctaaatca attatggtca
    19861 gccattcttg agggggatga aaaatttggc attgtaaata ttcaaaaatc tatatcaagc
    19921 tcttgcatcg cgggaattcc ggccatat
//
LOCUS       DAZSNG010000072        19701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZSNG010000072 DAZSNG010000000
VERSION     DAZSNG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.073"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHW4Z_002686"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHW4Z_002686"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR8882266.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHW4Z_002687"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHW4Z_002687"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR8882267.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHW4Z_002688"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHW4Z_002688"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR8882268.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHW4Z_002689"
     CDS             6038..6658
                     /locus_tag="ACHW4Z_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR8882269.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHW4Z_002690"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHW4Z_002690"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR8882270.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHW4Z_002691"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHW4Z_002691"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8882271.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHW4Z_002692"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHW4Z_002692"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR8882272.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHW4Z_002693"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHW4Z_002693"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR8882273.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHW4Z_002694"
     CDS             complement(10068..11069)
                     /locus_tag="ACHW4Z_002694"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8882274.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHW4Z_002695"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHW4Z_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHW4Z_002696"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHW4Z_002696"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR8882275.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHW4Z_002697"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHW4Z_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR8882276.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHW4Z_002698"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHW4Z_002698"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR8882277.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHW4Z_002699"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHW4Z_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR8882278.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHW4Z_002700"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHW4Z_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR8882279.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHW4Z_002701"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHW4Z_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR8882280.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5507 c         5032 g         4479 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttttc g
//
LOCUS       DAZSNG010000073        19279 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZSNG010000073 DAZSNG010000000
VERSION     DAZSNG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.074"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..850
                     /gene="yeaX"
                     /locus_tag="ACHW4Z_002702"
     CDS             <1..850
                     /gene="yeaX"
                     /locus_tag="ACHW4Z_002702"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR8882281.1"
                     /translation="GSHILVQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGG
                     SRFLHQQVKVGDRLTISTPNNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDW
                     QLHYCSRNPESCAFRAELVQHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGP
                     EALIEAVRSEAARLDIAADTLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQV
                     IENNKAAKVECLCREGVCGTCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGK
                     RLVLDL"
     gene            complement(904..2019)
                     /gene="rnd"
                     /locus_tag="ACHW4Z_002703"
     CDS             complement(904..2019)
                     /gene="rnd"
                     /locus_tag="ACHW4Z_002703"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR8882282.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(2101..3786)
                     /gene="fadD"
                     /locus_tag="ACHW4Z_002704"
     CDS             complement(2101..3786)
                     /gene="fadD"
                     /locus_tag="ACHW4Z_002704"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR8882283.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3992..4573)
                     /gene="yeaY"
                     /locus_tag="ACHW4Z_002705"
     CDS             complement(3992..4573)
                     /gene="yeaY"
                     /locus_tag="ACHW4Z_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR8882284.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(4613..5308)
                     /gene="tsaB"
                     /locus_tag="ACHW4Z_002706"
     CDS             complement(4613..5308)
                     /gene="tsaB"
                     /locus_tag="ACHW4Z_002706"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR8882285.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(5366..7276)
                     /locus_tag="ACHW4Z_002707"
     CDS             complement(5366..7276)
                     /locus_tag="ACHW4Z_002707"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR8882286.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            7408..7752
                     /locus_tag="ACHW4Z_002708"
     CDS             7408..7752
                     /locus_tag="ACHW4Z_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8882287.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            8174..8473
                     /locus_tag="ACHW4Z_002709"
     CDS             8174..8473
                     /locus_tag="ACHW4Z_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR8882288.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(8593..8772)
                     /locus_tag="ACHW4Z_002710"
     CDS             complement(8593..8772)
                     /locus_tag="ACHW4Z_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR8882289.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            8846..10207
                     /gene="pabB"
                     /locus_tag="ACHW4Z_002711"
     CDS             8846..10207
                     /gene="pabB"
                     /locus_tag="ACHW4Z_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR8882290.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10211..10789
                     /locus_tag="ACHW4Z_002712"
     CDS             10211..10789
                     /locus_tag="ACHW4Z_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR8882291.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            10973..12337
                     /gene="sdaA"
                     /locus_tag="ACHW4Z_002713"
     CDS             10973..12337
                     /gene="sdaA"
                     /locus_tag="ACHW4Z_002713"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8882292.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            12468..14066
                     /locus_tag="ACHW4Z_002714"
     CDS             12468..14066
                     /locus_tag="ACHW4Z_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8882293.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(14070..15620)
                     /gene="yoaE"
                     /locus_tag="ACHW4Z_002715"
     CDS             complement(14070..15620)
                     /gene="yoaE"
                     /locus_tag="ACHW4Z_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR8882294.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(15608..15766)
                     /gene="yoaL"
                     /locus_tag="ACHW4Z_002716"
     CDS             complement(15608..15766)
                     /gene="yoaL"
                     /locus_tag="ACHW4Z_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR8882295.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            16083..17054
                     /gene="manX"
                     /locus_tag="ACHW4Z_002717"
     CDS             16083..17054
                     /gene="manX"
                     /locus_tag="ACHW4Z_002717"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR8882296.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            17117..17917
                     /gene="manY"
                     /locus_tag="ACHW4Z_002718"
     CDS             17117..17917
                     /gene="manY"
                     /locus_tag="ACHW4Z_002718"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR8882297.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            17930..18781
                     /gene="manZ"
                     /locus_tag="ACHW4Z_002719"
     CDS             17930..18781
                     /gene="manZ"
                     /locus_tag="ACHW4Z_002719"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR8882298.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            18836..>19279
                     /locus_tag="ACHW4Z_002720"
     CDS             18836..>19279
                     /locus_tag="ACHW4Z_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR8882299.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         4702 a         5021 c         4826 g         4730 t
ORIGIN      
        1 aggaagtcac atcctagtgc agatgagcga tggtgataac cagtacagca atgcgtattc
       61 actactgagt tcaccgcata acacctctag ttatcagatt gccgttcgac tggaagaaaa
      121 ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat
      181 ctcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgt
      241 gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagttgcaac acagcgacat
      301 cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgctgagct
      361 agtccagcat ccgcaggctg agaaagtcca tttgcatcat tcatcaaccg gaacacgact
      421 ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata cctgtggccc
      481 cgaggcgcta attgaagcgg taagaagtga agctgcgcgt ctggacatcg ccgccgatac
      541 actgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgcattta ccctggtgct
      601 tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga
      661 aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga
      721 aacggcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg atgaagagcg
      781 tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt
      841 ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag
      901 attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg
      961 ccagccggaa atcagctccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag
     1021 cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat gcgtttcgct
     1081 cacgtcagta atcagcgact taatcgcttt aaacgcttta cgataaccag gcatgtccat
     1141 caggttaagc atcggctgcg gtaaggcatc ttccggcaat gtctgcgctt tttccaccag
     1201 cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag
     1261 ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt cacgcacgac
     1321 aaagttcacc gccagatcac gctctcgcgc cttgcgcagt cgccagtcgg ctaacagttg
     1381 cagacaggcc agttggcgtg tgcgtaactg ccaggcattg gtgatatcac gccaggcatc
     1441 ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc
     1501 aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata
     1561 ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg ccagccagtc
     1621 ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa
     1681 accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaagggttg
     1741 tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa
     1801 ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag
     1861 aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca gctgcgggta
     1921 ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaaga cacggacggc
     1981 ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgttttc
     2041 tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacgc
     2101 tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt
     2161 tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta ctttgtatcc
     2221 cgtgagctga cggcggcaaa aagtcaccag tgactcttcg gtaagcgatg gatctttttt
     2281 cactacgaag attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac
     2341 ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat aaacgttaaa
     2401 accggaaacc aaaatcatgt cttttttacg atcgacaatg cgcaggaatc cttcttcatc
     2461 catcaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatctcat ctgtagcatc
     2521 cggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg
     2581 acccggtggt acttcgttgt cgtcgtcatc caccagtttg gcttccgtcg atggcaccgg
     2641 caaaccgatg ctaccgctat gataatcaat atcatacggg ttaacgctca ccagcggcgc
     2701 gcactcggtc aggccataac cttccagcag atactgtccg gtcagtttca cccagcgttc
     2761 tgccaccact tgctgaacag gcatcccacc gcctgcggaa agatgcagac tggagaaatc
     2821 cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgccagtgat
     2881 agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcac gcgggttagt
     2941 gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa
     3001 aatgtgatac agcggcagtg ccgttactac cagttcttta cctggatgca gcaacggccc
     3061 ataggtggcg ttgacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc
     3121 tttcgccaca cctgtggtgc cgccggtgta ttgcaggaag gctaaatctt ccggcaccag
     3181 ttcgggtttg acgtactgca tccggtagcc gttatgcagt gcgctacgaa atgaaatggc
     3241 atctggcaga tggtatttcg gcaccaagcg cttgatgtat ttaacaacga aattgactac
     3301 cgtgcctttt gccgtagata gctgatcgcc catacgggtc agaattacgt gctgaacggc
     3361 ggttttatca accacttttt ccagagtgtg ggcaaagtta gacacgataa caatcgccga
     3421 tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt
     3481 tacgacgatt atcccggcac gcaaaatgcc aaatagcgcc accggatatt gcaataagtt
     3541 aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc
     3601 ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc atcacctccc ccatattcac
     3661 aaacgcaggc tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg
     3721 ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt
     3781 cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa atgatataca
     3841 cgccgttaac ataatattaa ctcatcatac cagcttgata attacccaac gaaaaggttg
     3901 cgaagcgcgt cactatttat ttttatcttt accgtaagaa tgcagaaaca gcggaccagc
     3961 cgctgtttct gcattcttta aaaacaagca attattcggt tacaactgtt tgtactctcg
     4021 cggggccggg attataccag ccccatccgc cgtagccata gggccagcca cgaccgccat
     4081 aaaaccatgg atcaatcggc tgaggcggca taatcacctg ctgggttaaa tgccaacgtt
     4141 tgtaacccgt tacttgcatc accataaatt tatagggcgt attgccgatt ttgccgtcaa
     4201 ccgcaccggt gattggcccg actaccgtaa ccagttgtcc acggaaatcc accgggtcca
     4261 gaaaaccgtt cacatcggca taaatgcgac cgcgagaagg ttctcccagc gtcggcctag
     4321 ctccgctgtc cagcggtacg gtagcaattt ccaggcgggt tttcccttgc tggttttgta
     4381 ccgcaaccac tttgccacca aagcgtgcct cctgaccaac gtacagctgc ggcgcactca
     4441 tcacccgaac taaatcttgt tgcggcgtgg tactgctgcc tttaatggcg tccggcacag
     4501 tgacacaacc gctcagcatt agcgcaaacg tacctgccag tatgcctttg ataacattct
     4561 tttgaaccgc catgatgcga ctcctttttt tctcagggca tactcttaag attcattctt
     4621 tgcccggaag tttcttccat gcgacgttgt tacgcaaata aaccggctcg gcatgttcca
     4681 ccgccaccgt tttaccctcg gcaaacatct gacaagcaat cggcagcata tcttctgcag
     4741 cggggagtaa cacttcgcca tcgcgcaaaa ccagcccgct ctctttaccg agatccggcc
     4801 aggcttgcca gcccgtccct accgtcaccc attcgccgga aagctgttgc attcgttcat
     4861 gaacgagttc gggtttgagt acggcttcgg tttcttcacc gtgccagata ccgttttcat
     4921 cacgctgata ttcggcccag taaacttcgc ccattcgcgc atcaatggct gacagcacgc
     4981 gtgttgcgcc gtttttgcgc cacgcccctt gcgccatcgt cattagcgtg gagacgccaa
     5041 tcatcggtaa ttccgcgcca agcgccagcc cttgcgcgat gccaatacca atgcgcacgc
     5101 cggtaaagct accggggccg cgcccgtaag ccagagcgtt aatatcagtc agggaagttc
     5161 cgctggtggt caggatatcc tgcaccatcg gtaagattcg ttgagtatgt tcacgagggc
     5221 aaagctcaaa atgagcgttg acagtaccgt cgttccacag ggcgacagag caggcctctg
     5281 tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc ttagatcaat aaaaaggcgc
     5341 gcatcatacc atactccgta acaaattacc tggaggatgg tatcgcaagg aaacgaaccg
     5401 cacgggcaat gtcacgggtg cgtggcgcgg gcggcagact ggcgagaaac gtcgcgccgt
     5461 aaggacgcat caccagccga tagtcacaaa tcaccagcac gccacgatcg tcggcgtcgc
     5521 gaatcagtcg acctaccccc tgtttgagag taatgacggc atctggtagt tgcacttcat
     5581 cgaacgggtc gccaccgcgc aaacgacaat cttccatgcg cgcttttaac agtggatcat
     5641 ccggcgaggt aaacggcaat ttgtcgataa ttaccaatga caatgtatcg ccacgcacgt
     5701 ccaccccttc ccagaaacta ctggttgcca caagaagcgc attaccggca ctgacaaact
     5761 gctgcaacag ttggccttta ctggtttccc cctgcaacaa aacgggaagc gtcatggtag
     5821 cgcggaactg ctcggccaga tcgcgcatca tggcgtgcga ggtacaaagc ataaaacaac
     5881 gaccgttgtt agcttcgatg atcggtcgca gcattgccgc taactggcga gcagaacctg
     5941 gctggttggt ttgcggcaga ttgcgcggca cacagagtaa cgcctggcgg ctgtaatcaa
     6001 acgggcttgg caatagcaac gactccgcct gttctatccc aagccgcgag gtgaaatgat
     6061 gcagatcgtc gttcaccgac agcgttgctg aggtgaagat ccagctaccg ggcttttgcg
     6121 ccattaactc tttgaattta tccgccacgc tgagcggcgt gagagccaga gtaaaatggc
     6181 gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt gatctctttt agccgcttca
     6241 gccgtgtgcg atacaacgtg gcgcgctcaa atgccgcatc cagcaaggcg gaacgcccca
     6301 gtgacagttt cgccacatca taacaaagtt ccaaggtgtc atcgagcagt aaaaatgccc
     6361 gctgaatttg cggattagct aacagctcac gcaggttacc gcgataacct ggctcaccga
     6421 gttgcagacg aaaatcctgc gcactctggg caagacgatc agcgcacttt tgtaactgct
     6481 gggtgtcttt taattcggtg cggtaggcga tggtgatgtc ttttgccagg tcgagcagtt
     6541 gtcgactgga gagtgactga ccaaaatact ggctggcaat gtccggtagc tggtgggctt
     6601 cgtcgaagat catgacgtcc gcttcaggga tcaactcgcc aaatccactc tctttaacca
     6661 ccatatccgc cagaaagaga tgatggttta ccaccaccac atcggcgtcc atcgcttttt
     6721 tacgtgcttt gaccacaaag caatctttat acatcgggca gtcgctgcca agacagttgt
     6781 cgttggtgct ggtgaccagc ggccacgcct gtgaatcttc cgccacgctg acgcaggtgc
     6841 tgatatcacc atcgactgtt tgattagacc aggagcgcag caggatcaca tcgcttaaga
     6901 tttgtaccgg cagatcgccc cccgccagcg cctgctgttc gagacgttcg aggcagaggt
     6961 agtttgagcg ccctttcagc agcgccacgt tgcccgtata tttcaatgcc tttgagactg
     7021 ttggcaaatc gcggctgtag agctgatcct gcaacgcttt tgagccggtc gagataatga
     7081 ctttcttttt cgcccgcagc gcaggagcca ggtaagcgta ggttttgccc gtaccggttc
     7141 ctgcttccac caccagcggc tggccttttt ctatcgcctg ggtgacggct accgccatct
     7201 gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga ccgtctggtg
     7261 caaaatcgtc cgtcacacta ccccctgttg atttgaacag ggattatgtc aggatgaggg
     7321 cgctttcgcc agttgaagtg gtgacggcaa cctcacattg tggcagtctt tgcagcacga
     7381 aaatggaagt ataatgagga caaaatgatg actatcgttc gtatcgatgc tgaagcccgc
     7441 tggtctgatg tagtaatcca caacaacacg ctctactaca ctggtgtacc ggaaaacctc
     7501 gacgccgatg cctttgagca aaccgccaac acgctggcac agattgacgc cgtgctggaa
     7561 aaacagggca gcaataaatc gagcattctg gatgccacca ttttcctggc cgataaaaac
     7621 gacttcgcgg cgatgaataa agcgtgggat gcctgggttg tcgcgggtca tgcgccggtg
     7681 cgctgcacgg tacaagcggg tttgatgaac ccgaagtata aagttgaaat taagattgtg
     7741 gctgcggtgt aagctttatc gaagcaaaat aagtcagacg ataatttatc gataatactg
     7801 gtcggtttta cataaatcga ccagagaata agatattact gttgatgctt ctgctaattc
     7861 attatttata tttataattt caattttatc tataaaacta actatataaa taaacataac
     7921 catgccgttg tgttcgccct ttgattagca actctggttt tctttcttag ctgaacaatc
     7981 tggttatagc gtgcgggctg accgttaatt ttctccatct tactggataa cacaccatga
     8041 gctgctacct ttaaaacacc tgaatgtcaa caggttaact cgtgccatat cgttataatc
     8101 cctttgatat tagatgcaaa ttaaggtcat atatagcctt atcgataaca tggttaattt
     8161 taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga
     8221 tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg gcgaagggaa tctttaaata
     8281 tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga
     8341 aggctggaac cctgggttca cggaaaaaat ggttggatgg gcaaaaaaaa tggagacagg
     8401 tgaacgctct gtgattaaaa atcctgaata cttttcaaca tatatgcagg aagaactaaa
     8461 agcactggtc tgagttaaat ttatatcagc ataaatgggt caggacgctt ttaatcacat
     8521 ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg cttctataac gaaatcgata
     8581 cggattagtg tgttactcgt cttcatcctc aaaacgcgcc acgattagct cgccggagtg
     8641 gttggcacgc agctcttctg ccaccagcgc gattgcctgg ccgctactca tcccttgtgc
     8701 catcagttcc tggatccgct cgacagcttt ttgctgctgt tcatggatga gtgaaggtaa
     8761 acctgcaaac attgttaact cctgctaaat tgttggcgct aattatttca tgctacccgg
     8821 cacatagcca gtagagtcag gactgatgaa gacgttatct cccactgtga ttactttacc
     8881 ctggcgtcag gacgccgctg aattttattt ctcccgctta agccacctgc cgtgggcgat
     8941 gcttttacac tccggctatg ccgatcatcc gtatagccgc tttgatattg tggtcgccga
     9001 tccgatttgc actttaacca ctttaggtaa agaaaccgtt gttagtgaaa gcgaaaaacg
     9061 cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcagacat
     9121 tcgcccaacg cataacgaag atttgccatt tcagggcggc gcactggggt tgtttggcta
     9181 cgatctgggc cgccgttttg agtcactgcc agaaattgcg gaacaagata tcgttctgcc
     9241 ggatatggca gtgggtatct acgattgggc gctcattgtc gaccaccagc gtcatacagt
     9301 ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt
     9361 ctcgccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga cccacgagca
     9421 gtacggcgaa aaatttcgcc aggtacagga atatctgcac agcggtgatt gctatcaggt
     9481 gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca
     9541 gcttaatcag gccaaccgcg cgccatttag cgctttttta cgtcttgaac agggtgcaat
     9601 tttaagcctt tcgccagagc ggtttattct ttgtgataat agtgaaatcc agactcgccc
     9661 gattaaaggc acgctaccac gcctgcccga tcctcaggaa gatagcaaac aagcagaaaa
     9721 actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa
     9781 tgatatcggt cgtgttgccg tagccggttc ggtaaaagta ccagagctct tcgtggtgga
     9841 acccttccct gccgtgcatc atctggtcag cactataacg gcgcgactac cagaacagtt
     9901 acacgccagc gatctgctgc gcgcagcttt tcctggtggc tcaataaccg gggctccgaa
     9961 agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgtaatg cctggtgcgg
    10021 cagcattggc tatttgagct tttgcggcaa catggatacc agcattacta tccgcacgct
    10081 gactgccatt aacggacaaa tatactgctc tgcgggcggt ggaattgtcg ccgatagcca
    10141 ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga
    10201 gaagtaagac gtggaatacc gtagcctgac gcttgatgat tttttatctc gctttcaact
    10261 tttgcgcccg caaattaacc gggaacccct aaatcatcgt caggctgccg tgttaatccc
    10321 catcgtccgt cgaccgcaac cggggttgtt gctgactcag cgttcaattc atctgcgtaa
    10381 acacgctgga caagtggcat tccctggagg tgcagtcgat gacacggacg catcagttat
    10441 cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata ccgccttccg ccgttgaagt
    10501 tatcggcgtg ctgccgcccg tcgatagcgt aactggctat caagtaaccc cagtggtcgg
    10561 cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga
    10621 aatgccgctc gcccaggcat tacatctggg tcgttatcac cctttagata tctaccgccg
    10681 tggcgattca catcgggtat ggctgtcctg gtacgaacag tattttgtat ggggaatgac
    10741 cgcaggcata attcgtgagc tggcgctgca aattggtgtg accccctgac tatacttatc
    10801 tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt
    10861 tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc
    10921 gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag
    10981 tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat
    11041 gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac
    11101 tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga
    11161 tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat
    11221 tcccggtttt attcgcgacg taaaagagcg cgaacgtctg ctgctggcac agggacggca
    11281 tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc tgccgctgca
    11341 tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca gcaaaactta
    11401 ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atgctgccaa
    11461 cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga
    11521 aaccggctat tcgctgtctg gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa
    11581 agagatcgac gagtatttcg cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg
    11641 gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg ccacgtcgtg cgtctgccct
    11701 gcgccggatg ttagtttcca gcgataaact gtctaacgat ccgatgaatg tcattgactg
    11761 ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc gccggtggtc gtgtggtaac
    11821 tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg ctggcttact atgaccactt
    11881 tattgaatcg gtcagcccag acatctatac ccgttacttt atggcagcgg gcgcgattgg
    11941 tgcattgtat aaaatgaacg cctctatttc cggtgcggaa gttggttgcc agggcgaagt
    12001 gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga
    12061 acaggtttgc gtggcggcgg aaattggcat ggaacacaac cttggtttaa cctgcgaccc
    12121 ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc
    12181 gattaacgcc gcacggatgg ctctgcgccg caccagtgca ccgcgcgtct cgctggataa
    12241 ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc
    12301 acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg cccatctgca
    12361 acggatgggc gaatttatac ccgctttctc gtctgctgta atattcccca ctacacttcc
    12421 actgttgcgt caggcgtttg tcgccatacg cttacagggt ggcccgcatg caaaaagcac
    12481 aacggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg
    12541 ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac
    12601 gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac
    12661 aggcaggtag caaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc
    12721 cattacgtaa acaggcggca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg
    12781 gcacacttta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg
    12841 ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat cgaccgtgcc ctgattaaag
    12901 gcagtccggt tttgattcaa tggacgcctg cagcgggcag tagcaatgct ggggtcatag
    12961 agatgattaa catcgactta ctgacggcaa tgctccttga gccacaactg ccgcaaatca
    13021 gtagcgccag cctgacggtg gacaaacggc atttgctcta tggtaatggg ctggtagatt
    13081 cccttccgca acctgaagac aatgaaaact atcaggtttc ttcgcaaagc tttcctttta
    13141 ccattaacgt aaatggtccg ggggctacgg cgctggcatg gcactatctt ccaacacaat
    13201 taccgctggc ggtgctgcta agtttactgg tgggctatat cgcctggctg gcgaccgctt
    13261 accggatgag cttttcccgc gaaattaatc tgggcctggc gcaacatgag ttcgaattgt
    13321 tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat tggtgtagag attttgctgc
    13381 gctggaataa tccgcgtcag ggctggattt caccggatgt gtttattcct atcgcggaag
    13441 aacatcattt aattgtgcca ctgacccact atgtgatggc agaaaccatt cgtcagcacc
    13501 atgttttccc gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc agccattttc
    13561 gccgtggtgt gctgataaaa gatctcaatc agtactggtt tagcgctcac ccgattcagc
    13621 aactgatcct cgaaatcacc gaacgcgatg ccttactgga tgttgattat cggattgccc
    13681 gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc ggcaacagct
    13741 cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa aattgataag tcatttaccg
    13801 cagctatagg ttctgacgcg gttaactcga cggtgaccga tatcatcatc gcgctggggc
    13861 aaagactgaa tattgaactg gtggcggagg gcgtggaaac gcaagaacag gcgaagtatt
    13921 tgcgccgtca tggcgtgcat attttgcaag ggtatttgta cgcacagccg atgccgctac
    13981 gtgattttcc caaatggctg gcgggcagcc aaccgccgcc cgcccggcat aatggacata
    14041 tcacgcccat tatgtcgtta cgttaacgct tactcatctt catcgtgcgc cggttgctct
    14101 ttaacaatgc gaaccagatc aacacgataa tcattggctt caaagatggt gatatgcagt
    14161 ggccctacat caatcacatc gcccacacgg ggaatgtgac catttgccga gatcacgagg
    14221 cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc
    14281 aaggcatgca aatctgtacc gccttttacc agccagccgt taccatcggt aatgatttcc
    14341 ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt ccagcacatc cagcggcgtg
    14401 accaagcctt gtaccacacc aaactcgttg gtcacgataa caaagctccc gcgagcgcga
    14461 cgcagcacgc ccagcaggtt gatcggatcg agggtttccg ggacgataat cgccggagac
    14521 gccgaagcaa tcgccgccac atcaacgccc tcttccagcg ccaccagcag ttctttagca
    14581 cgtacgatac cgatgatttc atccagttca ccgcgacata ccgggaacag actgtgcggt
    14641 gaagagagca gttgctcgcg gatttcatcg accccgagat tagcgtcaac ccagcttatt
    14701 tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta
    14761 atcatgtacc gttcttcttc ggcaaatgca ccttccggga tcggcatcgg gttatcggca
    14821 tcgtgctgaa cattggcctg acgtttcccg cccatcaaac gcaggatggc atcggcagta
    14881 cgcgctcgca gcggcaaagt cgactggtga cgaataaagt tgcgacgcgc aatctggtta
    14941 aacacttcga taatgatcga gaagccaatc gcggcataca ggtaaccttt cggaatgtgg
    15001 aaaccgaaac cttctgccac cagactcaga ccaatcatta acaggaagct cagacagagc
    15061 accaccaccg tggggtgctg gttaacgaat cgcgtcagcg gtttcgatgc cagcaacata
    15121 accgccatcg caatcactac cgccgccatc atcaccggca gatggttaac catccctact
    15181 gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga tgacaatctg tgtgacgacc
    15241 acccagaaac tggcgtagcc tttaccgtgg ccggaatcat gatcgcggtt ttccagccgt
    15301 tcatgcagtt cggttgttgc tttgaacagc aagaatatcc ccccgaacaa cataatcaga
    15361 tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg gtttggtcag cgtgaccatc
    15421 catgaaatca gcgacagcag ccccagacgc ataatcagcg ccagtgataa ccccagcaaa
    15481 cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa gaatggcgat gaagaccagg
    15541 ttatcgatac ccagcacaat ttcgagaaca acaagcgtga gtagccccgc ccaaattgag
    15601 gggtccatta agaattccat gacaagctcc tgcttaagga atagctattc gacgccagaa
    15661 ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg gcatagagtg ccagaaaggc
    15721 aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg atccatactg cgggctactg
    15781 ccctatactc catggttgtt aaacgggagt taaacatatc agagacgcct ctgatttggc
    15841 aaagatttac cttcctttgc aaacgaatgt gacaaggata ttttaccttt cgaaatttct
    15901 gataatcgaa agttaaatta cggatcttca tcacataaaa taattttttc gatatctaaa
    15961 ataaatcgcg aaacgcaggg gtttttggtt gtagccctta tctgaatcga ttcgattgtg
    16021 gacgacgatt caaaaataca tctggcacgt tgaggtgtta acgataataa aggaggtagc
    16081 aagtgaccat tgctattgtt ataggcacac atggttgggc tgcagagcag ttgcttaaaa
    16141 cggcagaaat gctgttaggc gagcaggaaa acgtcggctg gatcgatttc gttccaggtg
    16201 aaaatgccga aacgctgatt gaaaaataca acgctcagtt ggcaaaactc gacaccacta
    16261 aaggcgtgct gtttctcgtt gatacatggg gaggcagccc gttcaatgct gccagccgca
    16321 ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt taacattcca atgctcgtgg
    16381 aaacgttaat ggcccgtgat gatgacccga gctttgatga actggttgcg ctggcagtag
    16441 aaacaggccg tgaaggcgtg aaagcactga aagccaaacc ggttgaaaaa gccgcgccag
    16501 cacccgctgc cgcagcacca aaagcggctc caactccggc aaaaccaatg gggccaaacg
    16561 actacatggt tattggcctt gcgcgtatcg acgaccgtct gattcacggt caggtcgcca
    16621 cccgctggac caaagaaacc aatgtctccc gtattattgt tgttagtgat gaagtggctg
    16681 cggataccgt tcgtaagaca ctgctcaccc aggttgcacc tccgggcgta acagcacacg
    16741 tagttgatgt tgccaaaatg attcgcgtct acaacaaccc gaaatatgct ggcgaacgcg
    16801 taatgctgtt atttaccaac ccaacagatg tagagcgtct cgttgaaggc ggcgtgaaaa
    16861 tcacctctgt taacgtcggt ggtatggcat tccgtcaggg taaaacccag gtgaataacg
    16921 cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa actgaatgca cgcggtattg
    16981 agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa aatgatggat ctgatcagca
    17041 aaatcgataa gtaacgtatt gtgttgatta tcattcagtt ttcacactta agtcttacgt
    17101 aaacaggaga agtacaatgg agattaccac tcttcaaatt gtgctggtat ttatcgtagc
    17161 ctgtatcgca ggtatgggat caatcctcga tgaatttcag tttcaccgtc cgctaatcgc
    17221 gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc ggtattatta tcggtggtac
    17281 gctggaaatg atcgcgctgg gctggatgaa catcggtgct gcagttgcgc ctgacgccgc
    17341 tctggcttct atcatttcta ccattctggt tatcgcaggt catcagagca ttggtgcagg
    17401 tatcgcactg gcaatccctc tggccgctgc gggccaggta ctgaccatca tcgttcgtac
    17461 tattaccgtt gctttccagc acgctgcgga taaggctgct gataacggca acctgacagc
    17521 gatttcctgg atccacgttt cttctctgtt cctgcaagca atgcgtgtgg ctattccggc
    17581 cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag aacatgctga atgcgattcc
    17641 ggaagtggtg accaatggtc tgaatatcgc cgggggcatg atcgtggtgg ttggttatgc
    17701 gatggttatc aacatgatgc gtgctggcta cctgatgccg ttcttctacc tcggcttcgt
    17761 aaccgcagca ttcaccaact ttaacctggt tgctctgggt gtgattggta ctgttatggc
    17821 agtgctctac atccaactta gcccgaaata caaccgcgta gccggtgcgc ctgctcaggc
    17881 agctggtaac aacgatctcg ataacgaact ggactaacag gtgagcgaaa tggttgatac
    17941 aactcaaact accaccgaga aaaaactcac tcaaagtgat attcgtggcg tattcctgcg
    18001 ttctaacctc ttccagggtt catggaactt cgaacgtatg caggcactgg gtttctgctt
    18061 ctctatggta ccggcaattc gtcgcctcta ccctgagaac aacgaagctc gtaaacaagc
    18121 tattcgccgt cacctggagt tctttaacac ccagccgttc gtggctgcac cgattctcgg
    18181 cgtaaccctg gcgctggaag aacagcgtgc taatggcgca gagatcgatg acggtgctat
    18241 caacggtatc aaagtcggtt tgatggggcc gctggctggt gtaggcgacc cgatcttctg
    18301 gggaaccgta cgtccggtat ttgcagcact gggtgccggt atcgcgatga gcggcagcct
    18361 gttaggtccg ctgctgttct tcatcctgtt taacctggtg cgtctggcaa cccgttacta
    18421 cggcgtagcg tatggttact ccaaaggtat cgatatcgtt aaagatatgg gtggtggctt
    18481 cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg tttgtcatgg gggcattggt
    18541 taacaagtgg acacatgtca acatcccgct ggtggtttcg accatcaccg ggcaggatgg
    18601 acagacccgt gtcaccaccg tgcaaactat tcttgaccag ttaatgccag gccttgtacc
    18661 actgctgctg acatttgctt gtatgtggct gctgcgcaaa aaagttaacc cgctgtggat
    18721 catcgttggc ttcttcgtca tcggtatcgc tggttacgct tgcggcctgc tgggactgta
    18781 agactgtcgt acactaccgg ggccttttgg ccccgttttt ttatctggag gattaatgac
    18841 aatcacggac ctggtactga ttcttttcat cgccgcactc ctggccttcg cgatctacga
    18901 tcagttcatc atgccccgcc gtaacggccc caccctgctg gcaattcctt tgctccggcg
    18961 tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc attcttatct ataacaacgt
    19021 cacgaatcat ggcgcgctaa taacaacatg gttattaagt gcactggctc tgatgggttt
    19081 ttatatattc tggatccgca ttccgaagat catctttaaa caaaaaggtt ttttcttcgc
    19141 caatgtctgg attgaatata gccgaatcaa agcgatgaac ttgtcggaag atggcgtgtt
    19201 ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt cgaaatatcg acgatctgga
    19261 aaaaatttat aagcttctc
//
LOCUS       DAZSNG010000074        19029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZSNG010000074 DAZSNG010000000
VERSION     DAZSNG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.075"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1029)
                     /locus_tag="ACHW4Z_002721"
     CDS             complement(<1..1029)
                     /locus_tag="ACHW4Z_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882300.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAK"
     gene            complement(1131..2111)
                     /locus_tag="ACHW4Z_002722"
     CDS             complement(1131..2111)
                     /locus_tag="ACHW4Z_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8882301.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2119..2829)
                     /locus_tag="ACHW4Z_002723"
     CDS             complement(2119..2829)
                     /locus_tag="ACHW4Z_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8882302.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3046..4869)
                     /gene="typA"
                     /locus_tag="ACHW4Z_002724"
     CDS             complement(3046..4869)
                     /gene="typA"
                     /locus_tag="ACHW4Z_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR8882303.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5242..6651
                     /gene="glnA"
                     /locus_tag="ACHW4Z_002725"
     CDS             5242..6651
                     /gene="glnA"
                     /locus_tag="ACHW4Z_002725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR8882304.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6824..7873
                     /gene="glnL"
                     /locus_tag="ACHW4Z_002726"
     CDS             6824..7873
                     /gene="glnL"
                     /locus_tag="ACHW4Z_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR8882305.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7885..9294
                     /gene="glnG"
                     /locus_tag="ACHW4Z_002727"
     CDS             7885..9294
                     /gene="glnG"
                     /locus_tag="ACHW4Z_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR8882306.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9406..9516
                     /locus_tag="ACHW4Z_002728"
     CDS             9406..9516
                     /locus_tag="ACHW4Z_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR8882307.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9745..11118)
                     /gene="hemN"
                     /locus_tag="ACHW4Z_002729"
     CDS             complement(9745..11118)
                     /gene="hemN"
                     /locus_tag="ACHW4Z_002729"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR8882308.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11307..11816)
                     /gene="yihI"
                     /locus_tag="ACHW4Z_002730"
     CDS             complement(11307..11816)
                     /gene="yihI"
                     /locus_tag="ACHW4Z_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR8882309.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11935..12101)
                     /locus_tag="ACHW4Z_002731"
                     /pseudo
     CDS             complement(11935..12101)
                     /locus_tag="ACHW4Z_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12398..13030
                     /gene="yihA"
                     /locus_tag="ACHW4Z_002732"
     CDS             12398..13030
                     /gene="yihA"
                     /locus_tag="ACHW4Z_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR8882310.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13412..16198)
                     /gene="polA"
                     /locus_tag="ACHW4Z_002733"
     CDS             complement(13412..16198)
                     /gene="polA"
                     /locus_tag="ACHW4Z_002733"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR8882311.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16562..17494
                     /locus_tag="ACHW4Z_002734"
     CDS             16562..17494
                     /locus_tag="ACHW4Z_002734"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8882312.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17535..18965)
                     /locus_tag="ACHW4Z_002735"
     CDS             complement(17535..18965)
                     /locus_tag="ACHW4Z_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8882313.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4652 a         4804 c         4557 g         5016 t
ORIGIN      
        1 ttttgcttcg ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa
       61 gcccagcgta catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa
      121 cagtacgagc gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt
      181 caggatctta tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg
      241 gttaatgatg ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa
      301 tggaatgaag aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat
      361 aagccagatg gttttgtctc tcagcactga ggtcatactg gtgttttgcg atgctccgtc
      421 ttcttttttc acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaataat
      481 aatgcctacg gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa
      541 gccactgcca aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat
      601 gccacgccct gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg
      661 acttaccgac ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc
      721 ccagacaaag aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc
      781 tgtcgcaatg agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat
      841 agacagaaaa aagcccactg tggtgacaaa tgagtttacc gacatagcat taccaatttg
      901 accattggtc aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg
      961 taatttataa attgtaccgc cacacagtgt taatagacta aataacgccc atttcttttt
     1021 cgtgagcata acatccccta agcgattttt aaaatcactg ttttaaaggc aataagatac
     1081 cgaccattca tatggaatgg ttttattaac tgcgtatgag ttaaaactta ttagtggttc
     1141 agaagatggt ttgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga
     1201 ttaattgcat cttctttttc tttacgtaac cgcgcatcca gtaaatgatt ttctggtagg
     1261 ggtgtttcac tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg
     1321 aacgccgacg catagtaatc aacctcttct tccaggccat atgccgtcac ctgcggctca
     1381 tcatcgccaa ggcagataag gtgcgttaat aacgctttga atggcatatc accctgacct
     1441 gaaatacagg ctttatgacc caggccaccc tgttctttaa cgatcaaggc atctttaata
     1501 tggacctggg taatatgcgg tgccatcgtt tttaaggcgt cgatgggatg ctcatttgca
     1561 ttgatcatgt tagcaaaatc aaacagtaag gaaagagact ccatctcact ttctttgacc
     1621 agcgacacca gctcatgact ttttaaatct tcatgctgct caatcgtaaa agtcaggccg
     1681 ctgtcctgat acgtttcccg tacataggca atgtcgttag cgataatcga taatacgtcg
     1741 cgcaaattac cttcataaca tggataaaaa cgtacggacg ttgccccagt tttcaacgcg
     1801 atggcgacgg cttcgtcaat agatgcctta tctgaagcgc tggtttcaat atgaatatca
     1861 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
     1921 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactg
     1981 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
     2041 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
     2101 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
     2161 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
     2221 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
     2281 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
     2341 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
     2401 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
     2461 agttgatggc gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt
     2521 tttctcggta aacccccaag aaggctctcg cccttgctca attgcggccc gctgaaagct
     2581 cgccgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
     2641 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
     2701 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
     2761 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
     2821 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
     2881 aaaagaaagc aattaacggt gtatgacttt gcagataatt gtgacaaagc tctctattga
     2941 cgtaatcaca gacttataag ggagatactc gcattggtgt gcgctcacga caggtaaaaa
     3001 aaaacctgcc atcgctggca ggttttttat gactaaaaaa cgaaattaat cgtctttcgg
     3061 tgcgcggttg gcgcggcgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga
     3121 ggtcggagtc acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt
     3181 catgcggata ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt
     3241 cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat
     3301 aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcag
     3361 accgaacagc gcgaacgcga cagctttacc ctgaccgtta gagatcagta caccgttctg
     3421 acgctgaccc acttcgcccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag
     3481 accagtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg
     3541 gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc
     3601 tttacgttcg cccagcgcct gcatcacaga accctgatgc tgttcttcaa cgtccagcgt
     3661 cacgttttca tacggctctt gtttacgacc gtctatttca cggaagatta ctttcggacg
     3721 ggataccgcc agttcgaaac cttcacgacg catgttttcg ataagaacag agaggtgcag
     3781 ttcaccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc
     3841 aacgttgtgt accagttctt tgttcagacg atcgaggatc tgacgagacg ttacgaactt
     3901 accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc
     3961 atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat
     4021 gttcagttcg cccaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc
     4081 gatacgttcc agaccgaggt ggcccagcac tttaccgact ttcgcgttac gggttttgcc
     4141 ttcgctatcg atgatagtaa cctgctggtt cggcttcact ttaccacgct tgatgcggcc
     4201 aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg
     4261 accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctgat acagcggggt
     4321 catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta
     4381 aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac
     4441 ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat
     4501 cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc
     4561 gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc
     4621 accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt
     4681 gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc
     4741 catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt
     4801 gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt
     4861 ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg
     4921 cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga
     4981 tcgctttcac ggagcataaa aagggttatc caaaggtcac tgcaccaata tggtgcttaa
     5041 tgtttccatt gaagcactat attggtgcaa cattcacatc atggtgcagc ccttttgcac
     5101 gatgttaagc atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt
     5161 atctttttta cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgacaatga
     5221 ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac
     5281 gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca acacgtcact
     5341 atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc
     5401 tcgattggcg gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc
     5461 actgcagtga ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt
     5521 gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa
     5581 gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc
     5641 ttcctgttcg atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac
     5701 gatatcgaag gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt
     5761 ccggcagtga aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt
     5821 tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa
     5881 gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct
     5941 gacgaaattc agatctacaa atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc
     6001 gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg
     6061 tctctgtcta aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag
     6121 caggcgctgt actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca
     6181 aacccgacca ccaactctta taagcgtctg gtcccgggct acgaagcacc ggtaatgctg
     6241 gcttactctg cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa
     6301 gcacgtcgta tcgaagtacg tttcccagat ccggcagcta acccgtacct gtgctttgct
     6361 gccctgctga tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg
     6421 gacaaaaacc tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc
     6481 tctctggaag aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc
     6541 gtgttcacgg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc
     6601 gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag
     6661 ttgtactacc cggcgcacaa cgccgggatt tagttgccgt ggaaactttc agcccatctc
     6721 tgcatgggct tttttctccg tcaattctct gatgcttcgc gctttttatc cgtaaaaagc
     6781 tataatgcac taaaatggtg caacctgttc aggagactgc tttatggcaa caggcacgca
     6841 gcccgatgct gggcagatcc tcaactcgct gattaacagt attttgttaa ttgatgacaa
     6901 tctggcgatc cattacgcca accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa
     6961 attgtttggt acgccgttac cggaactgtt gagctacttc tcattaaata tcgagctgat
     7021 gcaagaaagt ctggaggcgg ggcaaggctt taccgataac gaagtgacgc tggtcatcga
     7081 tggtcgctcg catatccttt ctgtgacggc ccagcgtatg ccggacggca tgatcctgct
     7141 ggagatggca ccgatggata accagcgccg cttaagccag gaacaactac aacatgctca
     7201 acaagtagct gcccgtgatt tagtgcgcgg cctggcacat gagattaaaa atccgcttgg
     7261 cggtttacgt ggcgcggcgc agttactcag caaagcgtta cctgacccat cactactcga
     7321 atataccaaa gtgattatcg aacaggcgga ccggctgcga aatttggtcg accgtctgtt
     7381 ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt
     7441 ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg attacgatcc
     7501 cagcctgccg gaactggcgc acgacccgga tcaaattgaa caggtcttgc tgaatattgt
     7561 gcgcaatgcg ctacaggcgc tggggccgga aggcggtgaa atcattctgc gtacccgcac
     7621 cgcgtttcaa ctgaccttac acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga
     7681 agataacggg ccgggcattc cgcctcattt gcaggatacg ctgttttacc cgatggtcag
     7741 cggccgcgaa ggtggcaccg ggcttggctt atccatcgcc cgtaatttga ttgatcagca
     7801 ttcaggcaaa attgaattta ccagttggcc aggtcatacc gagttctcgg tttacctgcc
     7861 tatcaggaaa taaaggtgac gtttatgcaa cgagggatag tctgggtagt cgatgacgat
     7921 agttccatcc gttgggtgct tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca
     7981 tttgagaacg gcgcggaagt actggaggcg ctggcgagca aaacgccgga tgtgctgctt
     8041 tcagatatcc gtatgccggg aatggacggg ctggcgctgc tcaagcagat taaacagcgc
     8101 catccaatgc ttccggtcat cattatgacc gcacattccg atctggatgc tgccgtcagc
     8161 gcctatcaac aaggggcgtt tgattatctg cccaaaccgt ttgatatcga cgaagcagtg
     8221 gcgctggttg agcgcgctat cagtcattac caggaacagc agcagccgcg taatattcag
     8281 cttaacggcc caacgaccga tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt
     8341 attatcggtc ggctttcgcg ttcttctatt agcgtgctga ttaacggcga atccggcacc
     8401 ggtaaagaac tggtcgctca tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt
     8461 atcgcgctga atatggcagc tatcccaaaa gatttgatcg aatcagaact gtttggccac
     8521 gagaaaggcg cgtttactgg cgcgaatacc attcgtcagg ggcgttttga acaggccgat
     8581 ggcggcacgt tatttctcga cgaaattggt gatatgccgc tggatgtgca gacgcgtttg
     8641 ctgcgcgtgc tggcagacgg tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg
     8701 gatgtgcgga ttatcgctgc cactcaccag aatcttgaac agcgggtgca ggaaggtaag
     8761 ttccgtgagg atctgttcca ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc
     8821 gaacgtcggg aagatattcc ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa
     8881 ctgggcgtag aagcgaagtt gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg
     8941 tggccaggca acgtgcgcca gctggaaaac acctgccgct ggctaacggt gatggccgcc
     9001 gggcaggaag tgttgattca ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag
     9061 agtacttcgc aaatgcaacc ggacagttgg gcgacacttt tagcgcagtg ggcagacaga
     9121 gcgctgcgtt ccggtcatca aaatctgctt tccgaagcgc agccagagct ggagcggacg
     9181 ttactgacga ccgcgttgcg acatacgcag gggcataaac aggaagcggc gcggctactc
     9241 ggctggggcc gcaacactct gacgcgtaag ttaaaagagc tggggatgga gtgattcacg
     9301 gcttgtgtgt aaagattgat tattgagcgc aaattgctgg tattttgcgc tttactgttc
     9361 cgataagttc agtatgatct tgcccggaaa acggggagag tcattatgct ggaatcaata
     9421 attaatctgg tatcgagtgg cgcagttgac agccacacac cgcaaactgc cgttgctgcc
     9481 gtgctgtgtg ccgcgatgat tgggctgttt agctgaagct cgttgtttat gccggatgcg
     9541 gagaaagtcg taggcctgat aagacgcgac cagcgtcgca tcaggcatca tgctcagatg
     9601 cctgatgcgg cgtaaacgcc ttatccggcc tacaaaagca ataatttcaa caggttttgt
     9661 tgcccagcgc atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt
     9721 tgtaaacgaa gcgccattca ctctttaaat cacccgtgag aactgctgca tccgcgcttt
     9781 ctggcgcaga taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc
     9841 cgtcacctgt attcccttct catccacatc caccagccca tcttttgcta acggggcgag
     9901 cagcttgaga tcttccgcaa agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg
     9961 ggcgtaatcc agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc
    10021 atcacgcgtt agcgcaatac cacgccacag cgcattgcct tgttcataca cttgctgata
    10081 gtactgcttc aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga
    10141 aacgcccatc cccagcagat cggtatcgcc ctgagtggtg tagccctgga agttacgatg
    10201 cagcacgcct tcacgctggg ccaccgccag ctcgtcatca ggacgggcaa agtgatccat
    10261 accgataaac tgatagcccg attgcgtcag gaaggcgatg gtttcctgca ggatatcgag
    10321 tttttgctgc ggactcggca ggtcagcatc tttgattttg cgctgagcag caaaaatggt
    10381 cggcagatgc gcgtagttaa aaacgctcag acgatcgggg ttcagctccg ccacacgttt
    10441 cagggtaaag gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt
    10501 ggtggaggta aaaccaatct cacgcgcatg gttaagcagt gcaaagatga actcttcatc
    10561 ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatgctcag
    10621 gcgattaaag ccttcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc
    10681 gacttcgatc gaaatctccg catcggtatt gaaatggaag ttttcgcgca gcagcttcat
    10741 cagacggctg atttgcgctt tattcagata cgtcggcgtt ccgccgcccc agtgcaattg
    10801 gctgacgtga cgcccggcga acagcggtgc acgatggacg atttcttgct ccagcgcgtc
    10861 cagatactga tcggccttgt gctgctggcg agtaacaatc ttattgcaac cgcagaagta
    10921 acaaagctta tggcagaacg ggatatgtac gtagagagat aatggacgct caggatagcg
    10981 cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg
    11041 gtacgaggtg tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc
    11101 gatttgctgt acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc
    11161 ggcggatgcc cgtttcgggt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac
    11221 cgctgtagtt taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga
    11281 acgggaaggc ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct
    11341 tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc
    11401 aactcatcaa tacgatccag tttagcatcc acccaggatt gctcttcggc gctcagggtt
    11461 tcgcctgctt ccagacgttc cagcagcgca tccagacact catccgtttc cagtaattcc
    11521 aactccgcct gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact
    11581 ttttcagtca ctcccaatgg aataggggtt ttactgccaa tacgtggatc ttttggtgcg
    11641 ttctggcctt tgctgcctga cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga
    11701 ccacgacgtt ttttctgacg cttacggtca cgagcttcct gatccagctc ctcgcgtgtt
    11761 tttcgacgcg cttttgcatg acctttgctg cgtgagtttg aagatgatgg tttcataatg
    11821 gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag
    11881 caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg
    11941 ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc ggaacaatcc
    12001 gtgttgattc catttccgtt taattacgtc tccggacgtt tgtcttcctg acaatcctct
    12061 gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc
    12121 gctctatggg tgctacttta cgcctttgct ttaaacaaac aatcaaccgc catcagtatt
    12181 tgcacatttt cagagtttta tataaaagca attcactgat ttatatagct tatttgacat
    12241 gcatcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagcg
    12301 tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat
    12361 aatcgccaca aagctttgac tgacggagac gaccgctttg actaatttga attatcaaca
    12421 gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga
    12481 agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa
    12541 ccagaaaagc ctggctcgta cctcaaaaac cccagggcgc acccagctta tcaacctgtt
    12601 tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc
    12661 ggaagagatg aagcgcaaat ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag
    12721 cctgcaaggt ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca
    12781 gatgattgag tgggcggtag acagcaatat cgccgttctg gtgctgctga ccaaagcgga
    12841 caaactggca agcggcgcac gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc
    12901 gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa
    12961 gctgaggcag aaactggata cctggtttag cgagatgcag cctgtagaag aaacgcagga
    13021 cggcgaataa ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat
    13081 tcaatagatt acaattctac cgttggcctg ataaacatag cgcatcaggc attacggatc
    13141 ttttctttcg cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc
    13201 agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc
    13261 ctacgcgact aactttactc ttttttgttc agttcagaaa gcactttttg taattttttt
    13321 tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta
    13381 cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac
    13441 tccccacttc caccagcaac ggcacatcca gacgggtaca gttttccatc agttgatgaa
    13501 tctgcttcgc gacggcatca acatcatctt tatgaacttc aaataccagt tcatcgtgta
    13561 cctgcatgat catacgtaca cgcggttgct cagcctgtaa ccacgcatca acggcaatca
    13621 tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt
    13681 cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc
    13741 cgtccagcgt ttcaacgtag cccagctctt tcgcctgagc acgggtgcgt tccatatact
    13801 ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg
    13861 gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga
    13921 tcgctttcgc gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt
    13981 ctgccgccgt tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc aagcctttat
    14041 cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca
    14101 cataatcctc tggcgcaata aacgcctggc ggatacgacg accttcttcg ttacgcaccg
    14161 gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcagtt actgcctggt
    14221 gataagaggt atgcacacgc ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt
    14281 aagtcgactt cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt
    14341 ccagcgccag ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct
    14401 tcagcggttt aataccctgt ttttcaaaca gaatggtttg caactgcttg gtggaagaaa
    14461 ggttaaattc ttcacctgca atttcatgcg ctttcttttc cagctcagcc agacgcaagg
    14521 tgagctcttc agaatgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa
    14581 tgcgtgaaag caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt
    14641 tgtgtttctg cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt
    14701 cggcggcgta acgtccggcc tcttcgaggg caatctggtt aaaggtcagt tgatttttgc
    14761 ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc
    14821 tgtccatatc gtgacgcccg gcaacgctat tgagaatgta ggactccagc atggtatcaa
    14881 acgcaatccc acgcagttca atgccgtaat tcgccagaat accgcgatcg tatttcaggt
    14941 tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac
    15001 gctcgtgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg
    15061 ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc
    15121 tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt
    15181 tcagtgtttc ttcatcaagg atagtgacgt agttgtcata agaaatcacc gttgccgtca
    15241 cttctggcgc ttcgtctgca acactggttt cctgaggctt cgcggctgtt tttgcccctt
    15301 tggcctgtaa ccatttgccc acttcgacat cagcagtcca gcgtttgaac tcatactttt
    15361 tgaacagccc caacaactct tccgctgccg gttgctgcac ttccagttgt tcacaggtca
    15421 gctccagttc aacatcggtt ttaatcgtcg ccaactggta tgagagataa gcaacttctt
    15481 tgttttgctc gagcttcgct gccattgttt tcgcgccacg gaagctcaac ccagcaattt
    15541 tttctggctc ggcatacagc gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg
    15601 ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc atcagcgcca
    15661 ggaagtcgat gatcaattct ggcggcacgc cgtacttatt caccacctct tccggtccga
    15721 ggatggtatt cgtcatggta ttgataagcg taatatttgg cgtcaccagc tgcgccatat
    15781 ctttatcgcc agtgctaatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag
    15841 taccgataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt
    15901 taaccatcgc gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat
    15961 gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca
    16021 ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat
    16081 acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc
    16141 gataaagata agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa
    16201 tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat gaaaacatga
    16261 gtttttcacc gcttttcgtg aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc
    16321 atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat catgcttttt
    16381 acagcatgat gtttgtcttt gtttttcatg cagttaaaaa taaatccatc cctaataatt
    16441 gagataatgg gattttttct aatgtggata gatatgaatt atttttctcc ttaaggatca
    16501 tccgttattt gggtcatttt ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc
    16561 tatggctaac cttttgaata aattcattat gacgagaata ctcgctgcga taaccctttt
    16621 gctgagtatc gtattgacca ttttggtcac tattttttgt tccgtcccga tcatcattgc
    16681 cgggattgta aaactattgc tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg
    16741 tgattttatg atgtattgct ggtgtgaagg tctggcggta ttactgcatc tgaacccaca
    16801 cctgcaatgg gaagttcacg ggctggaagg gttaagtaag aagaactggt atctgcttat
    16861 ttgtaaccac cgtagttggg cagatattgt cgtactgtgc gtgctgtttc gtaagcacat
    16921 tccgatgaat aaatatttcc tcaagcaaca gctggcctgg gtgccatttc ttggcctggc
    16981 gtgctgggcg ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt tattacgtca
    17041 tccggagcga cgtggtcaag atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct
    17101 gcatcccacc accattgtca atttcgttga aggctcccgt ttcacgcaag aaaaacatca
    17161 gcaaacccac tccacttttc aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc
    17221 gctaaatgta ctgggtaaac aattcgataa actgcttaat gttacgctgt gttatccgga
    17281 caataaccgt cagccgttct tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca
    17341 tgtggattta cagcctattg ccgatgagtt acatggcgac tacatcaatg acaaaagctt
    17401 taagcgccat tttcagcaat ggctgaactc attgtggcag gaaaaagacc ggcttctgac
    17461 gtctctcatg tcatcacagc gtcagaataa gtaagtttta ctcgcctctg aatgagcaga
    17521 ggcgagtgag tattttaatg aacaaaacgt ccggcacgag acataaattc ttcttcgctc
    17581 atctcctgtc cgttaaaaac aactttaccc ggtgtataac gcagctgcag cgaggcggta
    17641 ttgttgtcaa tcgtgattaa ctgaaacatc tgacctaatg tcatcatccc gctgatttgt
    17701 ttatcagcct gcttttgcgc tttttccgca tccattcctt cagataaatt aagctgtttt
    17761 gcggtttctg tgacgacatt aagcggtaac ttcacatcaa aattgagcga tttgatatct
    17821 ttgtttgtgg atgatgaaga tttggctggg tcggcaatac tgatatccag attggcattt
    17881 agttcgccga gtgcgttctt ccatcttacc ggttgtttaa tggtcggctc acttttttgt
    17941 aataacggta aatactcttt gaacaatgca gcattcactt cttgcagagc aacttcatcg
    18001 tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata ctggatgata
    18061 aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc accactaccg
    18121 agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac attgatataa
    18181 tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat ttgggcaacg
    18241 tcttccccgt gattgataat gttaatctga ttaaatttcg actcgctttc accaaccgga
    18301 aatttctttt cttctaatcg cgccagagaa tcaatggtaa aactttttgc cgttactttg
    18361 ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc caggttagac
    18421 aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa ttttccatac
    18481 tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc
    18541 gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ttcattattc
    18601 actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg
    18661 atgggcaacg gaccgtgctc aacatccaca tcaaatacaa ctttctggcc tggcttgata
    18721 ttgagatcgg gtgcaccgtt atcgaatgtc atttgcattt gaaaacggga attaaaaaag
    18781 cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc tttctttttc
    18841 gcatcgttaa aatctttaat aaacttttcg ataccaggct gaatttgcgt acctgtgtac
    18901 catgtgccac caccccagat tacggctaac gcaacaataa cacctgtagc tgacttacgt
    18961 atcatataaa tattccctga taaaacagca aaactttgaa tatccactta tgctgaaata
    19021 atgtccagc
//
LOCUS       DAZSNG010000075        18803 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZSNG010000075 DAZSNG010000000
VERSION     DAZSNG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.076"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(139..3654)
                     /locus_tag="ACHW4Z_002736"
     CDS             complement(139..3654)
                     /locus_tag="ACHW4Z_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR8882314.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="ACHW4Z_002737"
     CDS             complement(4107..5369)
                     /locus_tag="ACHW4Z_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8882315.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="ACHW4Z_002738"
     tRNA            complement(5567..5642)
                     /locus_tag="ACHW4Z_002738"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="ACHW4Z_002739"
     CDS             complement(5748..6455)
                     /locus_tag="ACHW4Z_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR8882316.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6608..6691
                     /gene="yqgH"
                     /locus_tag="ACHW4Z_002740"
     CDS             6608..6691
                     /gene="yqgH"
                     /locus_tag="ACHW4Z_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR8882317.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="ACHW4Z_002741"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="ACHW4Z_002741"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR8882318.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACHW4Z_002742"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACHW4Z_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR8882319.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACHW4Z_002743"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACHW4Z_002743"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR8882320.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="ACHW4Z_002744"
     CDS             complement(11639..11914)
                     /locus_tag="ACHW4Z_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR8882321.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACHW4Z_002745"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACHW4Z_002745"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR8882322.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="ACHW4Z_002746"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="ACHW4Z_002746"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR8882323.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="ACHW4Z_002747"
     CDS             13874..14200
                     /locus_tag="ACHW4Z_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR8882324.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="ACHW4Z_002748"
     CDS             14384..15103
                     /locus_tag="ACHW4Z_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR8882325.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="ACHW4Z_002749"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="ACHW4Z_002749"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR8882326.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="ACHW4Z_002750"
     CDS             16442..17404
                     /locus_tag="ACHW4Z_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR8882327.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="ACHW4Z_002751"
     CDS             complement(17505..>18803)
                     /locus_tag="ACHW4Z_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR8882328.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAZSNG010000076        18671 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZSNG010000076 DAZSNG010000000
VERSION     DAZSNG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18671
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.077"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            375..578
                     /locus_tag="ACHW4Z_002752"
                     /pseudo
     CDS             375..578
                     /locus_tag="ACHW4Z_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            597..3983
                     /locus_tag="ACHW4Z_002753"
     CDS             597..3983
                     /locus_tag="ACHW4Z_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR8882329.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3986..4366
                     /locus_tag="ACHW4Z_002754"
     CDS             3986..4366
                     /locus_tag="ACHW4Z_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882330.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4421..4708)
                     /locus_tag="ACHW4Z_002755"
     CDS             complement(4421..4708)
                     /locus_tag="ACHW4Z_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR8882331.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4837..5346
                     /locus_tag="ACHW4Z_002756"
     CDS             4837..5346
                     /locus_tag="ACHW4Z_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR8882332.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            5343..6761
                     /gene="phrB"
                     /locus_tag="ACHW4Z_002757"
     CDS             5343..6761
                     /gene="phrB"
                     /locus_tag="ACHW4Z_002757"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR8882333.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6911..8392)
                     /gene="dtpD"
                     /locus_tag="ACHW4Z_002758"
     CDS             complement(6911..8392)
                     /gene="dtpD"
                     /locus_tag="ACHW4Z_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR8882334.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8663..9406
                     /gene="ybgI"
                     /locus_tag="ACHW4Z_002759"
     CDS             8663..9406
                     /gene="ybgI"
                     /locus_tag="ACHW4Z_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR8882335.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9429..10085
                     /gene="pxpB"
                     /locus_tag="ACHW4Z_002760"
     CDS             9429..10085
                     /gene="pxpB"
                     /locus_tag="ACHW4Z_002760"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR8882336.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            10079..11011
                     /gene="pxpC"
                     /locus_tag="ACHW4Z_002761"
                     /pseudo
     CDS             10079..11011
                     /gene="pxpC"
                     /locus_tag="ACHW4Z_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            11001..11735
                     /gene="pxpA"
                     /locus_tag="ACHW4Z_002762"
     CDS             11001..11735
                     /gene="pxpA"
                     /locus_tag="ACHW4Z_002762"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR8882337.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11771..12562
                     /gene="nei"
                     /locus_tag="ACHW4Z_002763"
     CDS             11771..12562
                     /gene="nei"
                     /locus_tag="ACHW4Z_002763"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR8882338.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12559..13605)
                     /locus_tag="ACHW4Z_002764"
     CDS             complement(12559..13605)
                     /locus_tag="ACHW4Z_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR8882339.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13757..14833)
                     /locus_tag="ACHW4Z_002765"
                     /pseudo
     CDS             complement(13757..14833)
                     /locus_tag="ACHW4Z_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14830..15561)
                     /locus_tag="ACHW4Z_002766"
     CDS             complement(14830..15561)
                     /locus_tag="ACHW4Z_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8882340.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15576..18035)
                     /locus_tag="ACHW4Z_002767"
     CDS             complement(15576..18035)
                     /locus_tag="ACHW4Z_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8882341.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(18086..18652)
                     /locus_tag="ACHW4Z_002768"
     CDS             complement(18086..18652)
                     /locus_tag="ACHW4Z_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8882342.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4614 a         4473 c         5154 g         4430 t
ORIGIN      
        1 gttgttaata aaaagcttat agatttatag ttgataatta aaacgcatcc gatatttcag
       61 ctttttctat gcaagataca attttataag gtgtccattt ttatatggag gcggtagcgt
      121 gtctgttact agtggtttta tttgcatagt gaatatattg ggattgttaa atacataact
      181 atgtatccgc tctggtcgtt gtaaatattg ctaattgctt aatattatta gtgattatga
      241 ctggaaagat aaagtgtgct gtaaagcggt gatgggtatt acattcatga ttaaaaaaac
      301 agaacctgtc aataaataat tcctttatta aaacggacta tttacattat gacggaatat
      361 tatctcaacg aggcggtttg gaaatacaga gccagtttct ccggggtaac gagcaggtgt
      421 accagtgtca ggtggcattt gtattgccgg gtgagcgggt catgatggcc ttcacctacg
      481 ccagaaccac accgctgacc ccggcagata tgacgcgctg ggctgaaatt aaaaagaatc
      541 tgcgcttcag gatgcggcag gaagtcagga cgaattaatc atacagggaa cgggacatgg
      601 aagaggcatt ctgggcggca agacagggtg atgccctgct gcatacgtcg tttatggcag
      661 atgtgctggg ggcggtgctg gaggtggccg caaatgtcgt tattgatgcc ctcattgtcg
      721 gcacaacctc gttactggcg acgtcagtgg ggatcacgat ggggtgcacg gccgtcgtgc
      781 tgaccggttt tgtggcgggt gtggcgatgg tgtacaccgg cgtgacagaa aaggtcagcg
      841 aagcctgttc agccctcgca aatatgcttt tcccgcctca gattgaaggt tacatcctca
      901 ccggctctgg tgacacatgg atcaacagca aacctgccgc gcgggcagcg gcgactgcgg
      961 catcgcggca ggacatagag gcgcaggaag cgcaggcgaa ggcagaacag gaagaagcgc
     1021 agcgtcagga ggatgccaga acgttccggg atgtggccgg ggaatatctg gaaatggcgg
     1081 gggccatagc cctggcggtt aacccggtga cagggcctgt gctgatgtcc cgggctctgc
     1141 acgaccagct cagtacggaa gaagggcgtt ctgaaatggt ggatggcgtg acacattttg
     1201 tgtcggaact atggcagccc actgtggcca gtgcggcccc gggatccacc ccgacccctg
     1261 acgataaaat cgactgccat aaacatcctt cctcactgac gcagtttctc gcacaaaaaa
     1321 aagccgcctt tctggatgac cccctgggaa cggcccttaa cctgctgaat ccgggcggta
     1381 tgctggaaac cgcctttcag ggagtgaatg cggttattgg cagcatcagt aatctgttca
     1441 aaggcgatga cgagccgccc gcagcggaat atatcgcgga aggcgcgcgg gatgtgcgta
     1501 tcaacagcca gccggcgaca cgcagcgggg cacgctgtac ctgcgaggcc agggtggtga
     1561 atgagccggg gaacggggcg tttgtgtcac cggatgtgcg cattggcggt ccgttactgg
     1621 tggtcaggga tatcagaagc ggcaggtcgc agataaccct ggtggcaacc gtggcgctga
     1681 tgtttcttcg cccgggcaaa atgctttcta aaatcgcctg ttttgctgcc agtgtcggga
     1741 tgagcatgct gacgcagaag gccaccagtg cccttccgca tccggtcaat gccgccaccg
     1801 gggccaaata cctggccgat gacgatgact tcgatttcag tctgccgggc cactttccgc
     1861 tggactggca gcgcgtgtac agcagtcgtg acgaacgtac ggaaggaatg tttggtcagg
     1921 gctggagtgt aatgtatgaa gtcagcctcg tgtgcacacc gggtagcgca gacgaaaact
     1981 gcatgacctt tgtgtcgggc atggggcgac gcttggatat ggaagccgta ctgccggggg
     2041 gcgggtttta cagccccggt gaagggctgg cggtaaggcg tggtgagcag gggcactggc
     2101 ttatcagcag cgatgacggg cagtgttttc tgtttgaggc tgacccgcac cacccgcagc
     2161 gccagcggct gaaaatgctg ggcgaccgta acagcaactg tctgaatctg tactacgacg
     2221 accggggccg cattgtggaa atcagcggcg aacagcagcg tccgtgcatc cgtctgtatt
     2281 acgaactggc ggcgcatccc cggcgggtga cacagattta tcagcatttc cctgagactg
     2341 cacctttact gctgcgtcgt tacagctatg acgaggccgg ccacctgaac ggcgtgtacg
     2401 acagcacggg gcacctgctg cgtgagtttg cttacgacga aaaccactgc atgacgctgc
     2461 accgccagcc gggcggcgaa gggtattatt accagtgggg ctggtatgaa ggtccggatg
     2521 acgcggcatg gcgggtgacg gggcaccaca cggacagcgg tgaacagtac cggctggcgt
     2581 gggacctggc gcagcgcagg ctgtgcgtga cggacggact ggggcgcaca cgttatcacc
     2641 agtgggatgc gcagaatcag gtgacggcgt accaggacga agccggacag gtgacgacat
     2701 tccgctggag cgacgaagaa cgactgttgc tggggatgac cgaccctcag ggcggcaaat
     2761 ggcgctatgt gtatgacctg caggggcata tcacggagac gcacgacccg ctggggcggg
     2821 tggagcaggc gcagtggcac ccggtgtggc accagccgga aacggaggtg gacgcggcgg
     2881 gaaacctgct ggggaggcgc gcgggggaaa gggccacggc agaaaacagc gtggtgccgt
     2941 tcaaccggct gatgtcttac cgtggtgtgc actaccgtta tgacgaattt ggccgtgcgg
     3001 tggaaaagga gggccggagc ggaacgcaga gttaccgtta tgatgcggaa caccggatgg
     3061 tggaagtgac gacagcgcgg gggacatacc ggtatgtgta cgatgcactg gggaggcgga
     3121 cggaaaaaca gcacatcagc cctgacggta agccgtacaa ccggacaaaa tttttatggg
     3181 acggaatgcg gctggcgcag gagagcaggc cggagggaac aggcagcctg tacatctaca
     3241 gggaccaggg gagttatgaa ccgctggcgc gggtggataa ggcgggcaaa gagggcccga
     3301 accggatact gtattttcac acggatgtga acggcgcgcc ggaagagatg acggacagcg
     3361 acgggaaaat cgtgtgggag acggggtatc aggtctgggg taacacgata caggagaagg
     3421 accacggcgg ggtggagcag aacctgcgct accagggaca gtacctcgac agggaaacgg
     3481 ggctgcacta caatctgcac aggtactatg atccggatgt cgggcggttc atggtaactg
     3541 acccgatagg gttgagaggc ggtttaaatc tctacagtta tgcacctaat cctctaaaat
     3601 atgctgatcc acttggatta actccatgtg ctgttagtaa ccaaaaagcg aatcgtttat
     3661 tagatagtag tgaaaccaaa gttactgtta ggtctagaag tgacgctgaa caattgttta
     3721 tggatagata tttaggtcat aattataaaa atatgacagg tgagtcaggc cccagcacta
     3781 agaacttaat ggaatatctt actgaaaata aaacaaaagc aggttcatat cattgggatg
     3841 atataaaaga tccttctgta acaaaacctt catatagagt gtcaggtcat gggccaggaa
     3901 atcctgatgg tgatttacca catttacaag ttcatcaaca tggtggaagt gttagacata
     3961 tatttttccc gtgggagaca taataatgaa caatctcaat acattattaa gtggaaaggt
     4021 tttgctcaaa gaaattagtc cattaatgcc tggatataga acatggattg aaatttcatt
     4081 gatcgatgaa ttaaaaccat cttatccttt tagattagat gaatatgcta tgatagggca
     4141 ttcaccttat gccaatgaat gtaataaaga tgaagctaaa tttaaactac gaatatcatc
     4201 atttttagct tctgacatag ataatgaata cgatccatca tatgattatg ttggaaaata
     4261 tgaaattatc gcttcactta atgagttaaa aactaggtta agtactcttc atgtaaactt
     4321 ggagcaattt ataaattcat cggaagatga tgagtatcca ttataataag acagaataaa
     4381 aagctaggaa ggttttaaat cttcccagct tttttaatat ttaactttcg ttcagcacac
     4441 taaacatccc atcccgcagt tcctgcacct cgtcactgtc agcgatgatg gtcaggcccc
     4501 cctccgaaat cttcaccgtg acaccccgtc cggtatcaaa tccggcctct ttcagccagt
     4561 cgcccttaag gtgcagactg ggatgttgtg aataataccg ggctataccg gttttgcggt
     4621 cctggtggcg ggctctgaca tagccgactt tatagcggcg ctgtgttttg gaaatagtga
     4681 cttctggcgt aatatcgtgt tcagccatga ttaactacct tgattagttg cttgcgggga
     4741 gcgcgctttc gtaatcttgc cctgctagcg gattaacttc acaaacatca tttaccaacg
     4801 cctacactta ctcctgtata ccgttcagga gcagtaatga atctacaacg atttgatgac
     4861 agcaccctaa tccgtatctt cgcccttcat gagttacatc gactgaaaga acatggctta
     4921 acgcgcgggg cgcttctcga ttatcacagc cgctataaac tcgtctttct ggcccattct
     4981 cagcccgagt accgcaaact tggcccgttt gtggctgata ttcatcagtg gcaaaatctg
     5041 gatgattttt acaaccagta ccgccaacgc gtaattgttt tgctttctca tcccgccaac
     5101 ccgcgcgatc acaccaacgt tttgatgcac gttcagggtt attttcgccc gcatattgat
     5161 tccacagaac gccagcagct ggctgcgctt atcgacagtt atcgccgtgg cgagcaacca
     5221 cttcttgcgc cgctgatgcg tatcaaacac tatatggcgc tttatcctga cgcctggctt
     5281 tcagggcagc gttatttcga actttggccg cgtgtgatta acttgcgcca ttcaggagtt
     5341 ttatgactac ccatctggcc tggtttcgcc aggatttacg tctgcacgat aatctcgcac
     5401 tggctgccgc ctgccgcaat tcgtctgcac gcgtgctggc gctgtatatc gctacaccac
     5461 gcaagtggga gacgcataac atgtcgccgc gtcaggctga acttatcaat gctcaactga
     5521 atgggctaca aatcgcgctg gcggaaaaag gtattccttt attgttccgt gaagtggatg
     5581 actttgtcgc cagtgtcgaa atagttaaac aggtgtgcgc ggaaaacagc gttactcatc
     5641 ttttttataa ctatcagtat gaggtgaatg agcgagcgcg ggatgtggac gttgaaagag
     5701 cgctgcgtaa cgtggtgtgt gaaggatttg atgacagcgt gatcctgccg ccgggggcgg
     5761 tgatgaccgg caatcacgag atgtacaaag tctttacgcc ttttaagaat gcctggctga
     5821 aacggctgcg ggaagggatg ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat
     5881 cgataaagcc cgcgccatcc attacgctga attatcctcg tcagtctttc gatactgcgc
     5941 atttcccggt ggaagaaaaa gcggcgattg cgcaattacg ccagttttgc gagaacggtg
     6001 ctggagagta tgagcaacaa cgagattttc cggcagtgga aggcaccagc cgtttgtccg
     6061 ccagcttggc aacgggcggg ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac
     6121 agccgcaggc gctggacggt ggggccggta gtgtctggct tagtgagctg atctggcgcg
     6181 agttctaccg tcatctgatg acgtattacc cctcgttgtg taaacatcgt ccatttattg
     6241 cctggacgga tcgtgtgcag tggcaaagca atcccgcaca tttacaggcc tggcagaaag
     6301 gcaaaacggg atacccgatt attgatgccg ctatgcgtca gcttaacagc actggctgga
     6361 tgcataaccg gctacggatg attacagcca gttttctggt gaaagatttg ttgatcgact
     6421 ggcgcgaagg cgagcgatat ttcatgtcgc agctgattga tggtgatttg gcagctaata
     6481 acggtggctg gcagtgggcg gcttctacgg gtactgatgc tgcgccgtat tttcgtattt
     6541 tcaatccgat aactcagggc gagaaatttg accgtgaggg cgagtttatt cgtcgatggt
     6601 taccggagct gcgcgatgta ccagggaaag cggtgcatga gccgtggaag tgggcgcaga
     6661 aagcaggtgt gaagctggat tatccgcaac cgatagtcga gcacaaagag gcgagagtac
     6721 aaacgttggc agcgtatgag gcggcgcgga aggggaaata acagacgcgt caggcaatcg
     6781 agcccagatg ccggatgcgg cgtgaacgcc taatccagcc tacaaatttg tgcaaattca
     6841 ataaattgca ggaaatacgt aggcctgata agacgcgtca agcgtcgcat caggcatcgg
     6901 tgctggccta ttatgactcc agcgccagcg cgcggtttct gaatttcagc gcctgataca
     6961 gccaaatcat cagtaccagt cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata
     7021 cttcaatata tgcattgatg gagtagttga tcgccccgga agcatcaaac gaagcctgcg
     7081 atgtctgatc ggcaatcacg cccgccagat agttcgcgat cgcgccagaa agcagcatgt
     7141 agatgccggt taatacgccg gtcacgcccg gaatttcaat gcgcgtaatt tgcgacatgg
     7201 caaccgggtc gataaacagt tccgcaaagc ccatcaccgc gaggcctaac accatcagtg
     7261 gcagagaaga gtgaccatac attgcggacc agcgggcgct taaggtcaga atgcagaatc
     7321 cggcgctcat caggccaagg ccaagagcaa attttcccca gatgcgcacg gtacgattac
     7381 ctgcgacgct ttctttaacc acccacgcca ggaacacacc gcacagcata actgcgaagg
     7441 cattaatcga ctggaacatc gcggtcggaa cggtataacc aaacatatcg cggttaacga
     7501 agcggtcgat ataaaggcta atcgagctac cgccctgttg tgcgaaggcc cagaacaaca
     7561 tactgaagaa ggtgagcgtc acaatcagcc ccagctcctt ccgctgtttc tggttttctg
     7621 ctttgcgata aatttttgcc agtacaccca ggccaataat agtcgcgaca attaaggcgt
     7681 ataccgacca ctctttccag aacagcacgg taatcagtgc aggcgttgcc accagcagaa
     7741 ccagcagcca tccccagttc ggcaggagaa agtttgtcgc acgcagtact tttttgttaa
     7801 cgccgcgggt atgagtgaaa tgacgattgc cacataagaa aatgaccaga cccgcgatca
     7861 ttccaaccgc cgccaggcca aagcccatcg cccagctgta ctcttcctgg gcgtaaccac
     7921 aggcgatagg tgcgataata gaccccacgt tacccgccgc atacatcagc gagaaaccgc
     7981 catcacgacg cggatcggtt ggctcataca gctcgccgag cagacagctt acgttagatt
     8041 taaacaggcc atagccgcag acgataatcg ccagggacag atagaggaat gacggatgga
     8101 tctcactggc acccagcacc acatgaccga tcgccatcaa caacgccccc agcatcaccg
     8161 ccatgcgatt gccgagaact ttatccgcca aaaagccacc gaggattggc gtgacataca
     8221 ccagcgaaca gtaggcgcta aataactcgt aggcgtgagt atcgttgtat tttagttgat
     8281 tggtgagata gagaatcagc agggcacgca tgccgtaaaa gctaaaatat tcccagattt
     8341 gcagcgcaac gacatagtaa atagcgcgcg gttgtgatgc gtgtttattc ataataattc
     8401 cacaattgac ggcaacgtca cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta
     8461 aaactttgat acagatctga ttatttatgc aatatgctgt ctgattgcat aaatatacat
     8521 tagctgaagc gtgatgatat aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat
     8581 atgtcaaaag attggctatc gacgaacagg cgatgtaacg ctatgttaac ggtgtagcta
     8641 tcagaatacg gaaggcgcga agatgaaaaa caccgaactg gaacaactga ttaacgaaaa
     8701 actgaacagc gcggcgatta gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga
     8761 gacggtgcaa aaaattgtta ccggtgtcac cgccagccag gctttgctcg atgaggcagt
     8821 gcgtctgggc gcggatgcgg tcattgtgca tcatggctac ttctggaaag gagagtctcc
     8881 ggtcattcgc ggaatgaagc gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct
     8941 gtatggctgg catttgccgc ttgacgcaca tcctgagctg ggcaataacg cacagctggc
     9001 ggcgttactg gggatcacgg tcatgggcga aattgagccg ttggtgccgt ggggagagct
     9061 gaccatgcct gtgccgggaa tggaactagc ctcctggatt gaagcgcgtc tgggacgtaa
     9121 gccgttatgg tgtggcgata ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg
     9181 cggcgggcaa agttttatcg atagcgccgc gcgttttggc gtggatgctt ttattactgg
     9241 cgaagtttct gaacagacca ttcattcagc ccgcgagcag ggattgcatt tttatgctgc
     9301 aggtcaccat gccactgaac gcggtggtat tcgcgcattg agcgagtggc tgaatgaaaa
     9361 taccgatctt gatgtgacct ttattgatat tcctaatcct gcataacgaa taatcagagg
     9421 gatcgaaagt gcaacgagcg cgttgttatc tgataggtga aacggcggta gtgctggaac
     9481 tggaaccgcc ggtgacgctg gctagccaga aacggatctg gcgactggcg cagcgtctgg
     9541 tggatatgcc gaatgtggtt gaagccattc ccggcatgaa caatatcacg gtgattttgc
     9601 gtaatcctga gtcgctggcg ctggatgcca tagagcgttt gcaacgctgg tgggaggaga
     9661 gcgaggccct ggaacctgag tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag
     9721 gcggaccgga tttggcggtg gtcgcggcgt attgcgggtt gagcgaaaaa caggttgttg
     9781 aattgcactc ctccgtggaa tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt
     9841 atctcgggag tttgccggaa caactacaca cgccacggcg cgctgaaccg cgcttgctcg
     9901 ttccggcagg ttctgtcggg atcggcgggc cgcagactgg tgtttatccg ctggcaacgc
     9961 cgggtggctg gcagttgatt ggtcatacct cactcagcct gtttgatccg gcgcgtgacg
    10021 aacccatctt attacgtccg ggagacagcg tgcgatttgt accgcagaag gagggaatat
    10081 gctgaagatt attcgtgcgg gcatgtacac cactgtgcag gatggcggtc gtcacggttt
    10141 ttgccagtcg ggtatcagcc actgtggcgc actggatatg cctgcgctgc gcattgctaa
    10201 cctgctggtg ggtaatgacg ccaatgcccc cgcgctggag atcacgctcg gtcagttaac
    10261 agttgagttc gaaactgatg ggtggtttgc cctgacgggt gcaggttgcg aagcgcggtt
    10321 ggatgataac gccgtctgga ccggctggcg attgccgatg aaagcaggcc agcgtttaac
    10381 gcttaaacgc ccgcagcacg ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt
    10441 tccgccggta atggggtcat gcagcaccga tctcaaagtg gggattggcg ggctggaagg
    10501 ccgtttactg aaggatggtg actgactccc gattggcaaa tcgaagcgtg attttatgga
    10561 agcgcagggc gttaaacagc tgctgtgggg caaccgcatt cgcgccttgc cggggccgga
    10621 atatcatgag ttcgatcgcg cctcgcagga tgcattctgg cgttcgccct ggcagcttag
    10681 ctcgcaaagt aaccgaatgg gctatcgttt acaggggcaa attttaaaac gcatcaccga
    10741 tcgcgaactg ttatctcacg gtttgttacc gggcgtggtg caggtgccgc ataacgggca
    10801 gccgattgtg ttgatgaacg acgcacagac caccggtggt tatccacgta ttgcctgtat
    10861 cattgaggct gatatgtacc atctggcgca aattccgctc ggtcagccga ttcattttgt
    10921 ccagtgttca ctggaagagg cactgaaagc gcggcaagat cagcaacgtt attttgaaca
    10981 attagcgtgg cggctgcaca atgaaaattg acctgaacgc cgatctgggc gaaggctgcg
    11041 ccagcgacgc agagctatta acgctggttt cctctgccaa tattgcttgt ggatttcatg
    11101 caggcgatgc gcaaaccatg caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga
    11161 ttggcgctca cccgagtttt cccgacaggg aaaattttgg tcgcagcgcc atgcagctgc
    11221 cgccggaaac cgtttacgcc cagacgctgt atcaaattgg cgcgctggca acgattgccc
    11281 gtgcgcaagg cggcgtaatg cgtcatgtca aaccgcacgg catgttgtac aaccaggcgg
    11341 cgaaagaagc acaactggca gacgccatcg ccagagcggt atacgcttgc gatccggcat
    11401 tggttctcgt tgggctggca ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga
    11461 caacgcgcga ggaagtgttt gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc
    11521 gaagtcagcc aggcgcgttg attgaaaacg aagaacaggc gttggcgcaa acgctggaga
    11581 tggtgcaaca cggcagagtc aaaagtatca ccggcgaatg ggcaacggtc gcggcgcaaa
    11641 cggtctgcct gcatggcgac ggcgagcacg ctctggcgtt cgcccgccga ttacgagcta
    11701 catttgccaa aaaggggatt gttgtcgcag cataaccccg cttaataaag aatgaaaaaa
    11761 ggatatcacc atgcctgaag gcccggagat ccgccgtgca gcggataacc tggaggcggc
    11821 gatcaaaggc aaaccactaa ctgatgtctg gtttgccttc ccgcagttaa aaacttatca
    11881 atcacaactt atcggtcaac acgttaccca tgtggaaacg cgtggtaagg cgttgttaac
    11941 tcattttccc aacggtttaa cgctctacag ccataatcag ctttacggcg tctggcgcgt
    12001 ggttgatacc ggcgaagagc cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc
    12061 tgacaaaacc attctgcttt atagcgcctc ggatattgag atgttgaggc cggaacaact
    12121 gaccacgcat ccgtttttac aacgcgttgg tcccgatgtg ctggatccga atctgacgcc
    12181 ggaggtggta aaggaacggt tattgtcgcc gcgctttcgt aaccgtcagt ttgcaggatt
    12241 actgctcgat caggcgtttc tggctgggct tggcaattat ttgcgggtgg agatcctctg
    12301 gcaggttggg ttgactggaa atcataaagc gaaagatctc aatgcggcgc aactggatgc
    12361 actcgcacac gcgttactgg agattcctcg gttttcttac gctacgcggg ggcaggtgga
    12421 tgagaataag catcatgggg cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact
    12481 gtgcgaacgc tgtggcggca tcattgagaa aaccacgttg tcctcgcgcc cgttttactg
    12541 gtgtccaggc tgccagcact aggccgaccg cttcggcgca taggttgaaa taaaccgcgc
    12601 aatggctggc cccgtcagca aaatactgaa caggcgcagg gtttgcatcg ccataataag
    12661 cgccatatcg gcattgctcc ctgcggcgat gaccgccacg gtatcaagcc cgccgggact
    12721 ggtggcgagg taggcagtca taaaatcaat atgcataaac cgggtcagtc cccacgccat
    12781 acctgcacaa atagccagca gagcaaaaat cgacagcagg atttgcggta gcgggcgtag
    12841 tgcccgcagt aagatttgtt tatcgaaacc aagaccaatc cgccagccaa ttgccatata
    12901 cgccatcgcc agcagccatt ccggtagttc gatggtgatg agctgaccag actggagcac
    12961 tgcgcccgcc agcatcggga tgagcatcgt gccggaagga agacgcagca tgcatcccgc
    13021 cgtaccggca acgactgcga gcaaaatcgt aagcaggaga ttaatgctca ctggcgggaa
    13081 ccagacgatt tgctggttaa ccgcttcagc gttatcaccc agcatcatgc gggtgaccag
    13141 aacagcggcc cccgcgacga acagcactcg cagatattgc ataaacgcca ccaggcgaat
    13201 atctgcgccg taatcttgcg ccatagcgac cattgccgct gcgccgcctg gagaagaacc
    13261 ccaggcaccg gtatttccag gcagtgagct atagcgcacc aataaccaac ccacgatggc
    13321 gctggaaagc agcgtcacca gtaaaatagc aagcacgatc ggccagttgg cggccagcgt
    13381 ggtgagaatc gaaccagtga ggttttgcgc aatcatgcag ccaagaatgg cctgcgcggc
    13441 gagaaaagcg gagcggggaa gttgcagagt aattccgcgc atactgaaga tgatcccggc
    13501 gatcatcggc ccaagtaata gcgccgccgg gagatgtacc gcgaggaaac caatagaaag
    13561 gagaagtgat agcacacata acattcccca ctgcaaaact ggcatccttc gctccctcat
    13621 tattttctct ttggtgtatc gatacagcat aaagaaagcc gggctaggaa aaaataacag
    13681 caatcaagga aaaggggaaa atcagcaatt ttctgaaaga gatgcccttt ccggctgcaa
    13741 agggcatcat ggcaaatcag ttatagttaa ttttaaaaag caccactgta tcgaagggcc
    13801 cagcctcgat gggggtacct gcaatggacg aaagttcagc tgtataggtt ttttcgtaga
    13861 ccaacatgtc gctggtgaaa tcggcgtatt cagcatattt attgaaggta taaggcgagg
    13921 cgttttcatc cagcaaacgt agcttcagac cattaccaat caacaatgct ttatcttctt
    13981 caaccaacgt ttcttcggta taaaaggagg aactgacttt aaatccgtcg gtgcattggt
    14041 cattttgtga ttttgtcgtc ttaatactga atgttttcga cattgtgtgg cgacgaatat
    14101 ccagcaaatt aaatttacca aaatcaatca tctgtgtttc cggtgaaatg gaaaaattga
    14161 cgctgcaatc cagcacgcga atgttttcca gaccagtaat atgatatttc aggttttggg
    14221 cagtagggtc ttcgttaacg ctaccggcac cgtcaaattg cacgacgata tagtcgctaa
    14281 gcgtactttg ataatcatgc ggcggcattt ctcgtatttt gacgtataac cgcatgcgag
    14341 cgagaaaggt tcgcgacatg tgaatattag cgggatcacc cgagcaaatt ttgttctgcc
    14401 atcccatcag gggtataaat gtcaatgttc ttactgtcga tacactggtt agtgtcgatg
    14461 cggctttttc cctggttggc gtcataatca acgccattgt aagttactcc gagttgataa
    14521 taacgatcct gcacccccgg gtaggggttt acccatacgt agacatgttc gctttcaaag
    14581 ttgccgttgg tattgttgtc acaatagacg ggaattttaa tatcgtcaga ttcccagatc
    14641 ttattttaat atcgtcagat tcccagatct tatcgccgag tttggcattg cctggtacag
    14701 caaaaggttg aatcgcttct gatttttcta ccgagccacc ggatgaacca aagtagcagt
    14761 tcagggcaag cgcagacttc aacgacagaa ataacagaca gatgacgagc aacactccct
    14821 tgccagcact catttcttcg cctccacggc ttgtaaatgg cattgtccgc tgcagttgag
    14881 cgtcgtcatg cgtaacgcgc cgtaatcatc catataaccg agataaaact ggctgccgct
    14941 ataatttccg gtgtttgtgt tgaccgtact aaaaggcgca accatcacgg ttttgaagcc
    15001 ggggagaacg cctttttcat ttcgtcccag gtaggcaatg gtcagataat acgcggtagg
    15061 atttttcagt gtcagttggt tgccctgctg gctgacctgt aactgcagtt cgaccttttc
    15121 tcctgctttc ttgcgtaatg cagccggtcg ccagaacacc ttgatacgac tctgaatggc
    15181 gacttgcaga acggcatggt cgctgttttt ttccggggcg ggaggaattt cacgcatatt
    15241 gtagaaaaat agcgtttcgc gatcgcccgg taattttgtg gtcgaggctt gctttactat
    15301 ccgtacctgt gttgttgctt tcggttccag acgttgtatc ggcggcaggg cgaccagaag
    15361 atcatcgctt ttctcccctt tttcattttc gagccaggaa taagcgaggt aggggagttt
    15421 atcgctacga ttatccaccc gcagactggt agctttatca ttagcgttaa acacaatacg
    15481 agtgcgatcg ggttgtagtg cagcgtggct gcacagactg gcaaccaaca acaaaagggg
    15541 tagtcctttc ataaatgtca ttttcatttc cttaattaat gacaaggtaa aatcaggcga
    15601 tttgcggtgt cttccatatg ttcaggaaga tggagcgagc aatgctggct atcaccccag
    15661 accacggtaa acttttgatt ttcagcgact ccgcttaacc aggcatgtcc ttcctcgcca
    15721 accatcccca cgctaatgcc gctgtcatcc tggcggatat ccgctccgag gggaggaaac
    15781 tgaccgctgg cgttgcgaat gatgacgtta acgtctttac cggaacggga agccagtgat
    15841 ttgtaaccta tcgcgccttc aatccacgtt tctttgataa cgttttctgc aactgttacg
    15901 ccgtcgggta agtcattcat gttcaccgcc acggtggaag gctggtaact ggaaatcaac
    15961 ggcaccaccg caatgccaaa atggttggtg tagtcgagat tgccctgaac cggaatatct
    16021 gccacgccat cggtgctgac catcaggcgt ggttcattgg tggagctgcg gcgatgaaac
    16081 gctgcaccat attgggttgc ggtgaaagaa ccgctccagc tgctgctgac ggaactgtaa
    16141 tcattggcgg cataggtacc agaaatatcc cactcacccg ctgaactcag gtgctgatag
    16201 ttaccgctca cctgggctcc attgtcagga cggtcggatt gcagtccggc agacatgccc
    16261 cagctattac gttcatccag cgtatcgttc cacgacatgc ggtgtgtggt gctgtggcta
    16321 ctgttttgca tgtcatagcc aacccgacca ccgttaccga aggggagcga aatcgacagg
    16381 taaatctggt tgtcgcgatc tttatcttcg taatgggttg tattgaatga cgtagaaata
    16441 gagatatctc tccagtcacc aatatcaaca ttaaaacctg ccgtgatgtt ggcggtcgtg
    16501 gaggcgtctg cattccacca ggtttgatgt agcaggttgg cgtaaagatt gaggtttagt
    16561 ggggtaatcg gttggcccac agataagctg atcgtctgtt tttcgtcctg cgcatcgctg
    16621 tcgttgtatt tgtgatccag atagttggcg tagctgtgaa aatgacgatc ggagaaacga
    16681 taagcggcga gggaaatcgt gctgttagtg gcatccactt gtttgctgta attaaaacgg
    16741 tagcttaagc cccgctcgtc ctgctgagta tcaaaatggc tactggccca cgtgacatca
    16801 aacgacagcg caccaagcca cagcatattt tgcccaatac ccattgcggc agaatggtag
    16861 tcatcaccag aaagcagcag gccgctgtac agcgaggtgt ttgacagcat cccccaggaa
    16921 acttcattgc taaaaaaggt ttcattttca gtttgatgtg acatggaggg ccgtggctga
    16981 cccgcggcca gtttatagcg aacctgtccc tgacgagtca ggaagggcgt cgatgctgct
    17041 gaaacctgga aattgttcac ccgaccatct tcttccgtca ctttgacatc cagtgtgccc
    17101 tgaacagact gattaaggtc gtcaatgata aatgggcctg gtgggacttt tttctggtaa
    17161 atgacgcggc ctgattgact gatcgtcacc gtggcattgg tctgtgcaat accgctaatt
    17221 tgtggggcgt agccgcgtag ttcccatggc aacattcgat cgtcacttgc cagtgccgcg
    17281 ccggtataag aaaaaccgtc gaaaatattg ggactaaaat ccgtttcgcc gagggttaat
    17341 tttgaaccta attgcggtaa cgggcgaaaa agataggtac gtgatatttc gcctgactgt
    17401 tcatggttat catcgctatc agtatgattc aactgataat cactgcgtaa gcgccatgcc
    17461 cccgtgttga ttccggctgt tccataggcg ttaagattag tgctgctgct gccatcctgt
    17521 gggcggtagc tgctggcaaa caagttgtaa tccatcagga caccggcgac accttctttc
    17581 catgtggagg gtggggtcca gttttctgag tgccacgcca gccaggcttg cggaatacta
    17641 atattcagtt gctgattggc ttgatcgaaa ttgaagagca tttcaggtcg ggagctaaaa
    17701 tcgacgcact gatttatcaa tggtaacgac tgacggactt caggttttaa gccaaattta
    17761 tcgaccagta aatcattgat gcacggaatg gttttatcgt catttttgtg ccagttaatc
    17821 ttttgctcgt tactgatttg gttattattt accgcaacgc taacaaaata ctcaccggga
    17881 gcaatgactc ctttttcctt taataatgaa atatcaatgc ggtcgcgcat tgatttatcg
    17941 agaacattca gattgaattc gaccgccaat gcgcaaggca tcgccacgac caggcaggat
    18001 acaaaagaga gtcgataaat attcacggtg tccatacctg ataaatattt tatgaaaggc
    18061 ggcgatagcg ccgccaaagt aatacttatt tataatccag cacgtaggtt gcattcgccg
    18121 taactgcgcc tggcgttaca ggtgtagcct gatcaatttg ttccatccag gcaaagaaat
    18181 tcagcgtttg ttcgctggag gtcggagcca ggtcgatatc tggagtagcg gtgcctaaaa
    18241 cgatattgct ttgatcttta ttcattaaac gtacgccgac gccggtggct tcacccgtgc
    18301 tggtgttgtt gagaatcgga tctgcgccgg tggttttcgc tgagctatca aaggtgactg
    18361 caactttaga aatcttgcca ccggaaccgt tgctggagtt ttccagatca cagttgacta
    18421 agagcaggtc gacagcaacg gcatcgctgt gatgtgactg attaatgtgg ctggtggtca
    18481 cctgaccgag ttcaacctct ttatcaatat catcctgatg tatttcacac ggtgcttcaa
    18541 taacttcacc tttaaagtga atttcaccgg atccttcatc agccgcataa acaggtgctg
    18601 tgaacagcag agatacggcc agtgcggcca atgttttttg tcctttaaac ataactgagt
    18661 cctttaacag a
//
LOCUS       DAZSNG010000077        18068 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZSNG010000077 DAZSNG010000000
VERSION     DAZSNG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.078"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            256..780
                     /locus_tag="ACHW4Z_002769"
     CDS             256..780
                     /locus_tag="ACHW4Z_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR8882343.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="ACHW4Z_002770"
     CDS             861..1535
                     /locus_tag="ACHW4Z_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8882344.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4013
                     /locus_tag="ACHW4Z_002771"
     CDS             1551..4013
                     /locus_tag="ACHW4Z_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR8882345.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4029..5062
                     /locus_tag="ACHW4Z_002772"
                     /pseudo
     CDS             4029..5062
                     /locus_tag="ACHW4Z_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="ACHW4Z_002773"
     CDS             complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="ACHW4Z_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR8882346.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="ACHW4Z_002774"
                     /pseudo
     CDS             complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="ACHW4Z_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6653..6925
                     /gene="rcnR"
                     /locus_tag="ACHW4Z_002775"
     CDS             6653..6925
                     /gene="rcnR"
                     /locus_tag="ACHW4Z_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR8882347.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7017..7113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7148..7936
                     /gene="thiM"
                     /locus_tag="ACHW4Z_002776"
     CDS             7148..7936
                     /gene="thiM"
                     /locus_tag="ACHW4Z_002776"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR8882348.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7933..8733
                     /gene="thiD"
                     /locus_tag="ACHW4Z_002777"
     CDS             7933..8733
                     /gene="thiD"
                     /locus_tag="ACHW4Z_002777"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR8882349.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8798..9615
                     /locus_tag="ACHW4Z_002778"
                     /pseudo
     CDS             8798..9615
                     /locus_tag="ACHW4Z_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9667..10413
                     /locus_tag="ACHW4Z_002779"
     CDS             9667..10413
                     /locus_tag="ACHW4Z_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8882350.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10387..11352)
                     /locus_tag="ACHW4Z_002780"
     CDS             complement(10387..11352)
                     /locus_tag="ACHW4Z_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR8882351.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11349..12352)
                     /locus_tag="ACHW4Z_002781"
                     /pseudo
     CDS             complement(11349..12352)
                     /locus_tag="ACHW4Z_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12392..13444
                     /gene="fbaB"
                     /locus_tag="ACHW4Z_002782"
     CDS             12392..13444
                     /gene="fbaB"
                     /locus_tag="ACHW4Z_002782"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR8882352.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13752..14606
                     /gene="gatY"
                     /locus_tag="ACHW4Z_002783"
     CDS             13752..14606
                     /gene="gatY"
                     /locus_tag="ACHW4Z_002783"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR8882353.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14635..15897
                     /gene="gatZ"
                     /locus_tag="ACHW4Z_002784"
     CDS             14635..15897
                     /gene="gatZ"
                     /locus_tag="ACHW4Z_002784"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR8882354.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15907..16359
                     /gene="gatA"
                     /locus_tag="ACHW4Z_002785"
     CDS             15907..16359
                     /gene="gatA"
                     /locus_tag="ACHW4Z_002785"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR8882355.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16390..16674
                     /gene="gatB"
                     /locus_tag="ACHW4Z_002786"
     CDS             16390..16674
                     /gene="gatB"
                     /locus_tag="ACHW4Z_002786"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR8882356.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16678..18033
                     /locus_tag="ACHW4Z_002787"
     CDS             16678..18033
                     /locus_tag="ACHW4Z_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR8882357.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4728 a         4112 c         4570 g         4658 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2641 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2701 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2761 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2821 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2881 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2941 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     3001 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     3061 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     3121 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     3181 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     3241 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     3301 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     3361 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     3421 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3481 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3541 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3601 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3661 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3721 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3781 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3841 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3901 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3961 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     4021 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     4081 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     4141 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     4201 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     4261 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     4321 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     4381 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     4441 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4501 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4561 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4621 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4681 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4741 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4801 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4861 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt
     4921 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4981 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     5041 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     5101 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     5161 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5221 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     5281 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     5341 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5401 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     5461 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5521 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5581 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5641 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5701 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5761 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5821 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5881 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5941 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     6001 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     6061 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     6121 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     6181 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     6241 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     6301 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     6361 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     6421 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6481 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6541 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6601 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6661 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6721 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6781 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6841 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6901 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6961 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     7021 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     7081 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     7141 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     7201 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     7261 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     7321 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     7381 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     7441 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7501 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7561 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7621 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7681 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7741 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7801 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7861 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7921 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7981 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     8041 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     8101 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     8161 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     8221 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     8281 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     8341 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     8401 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     8461 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8521 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8581 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8641 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8701 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8761 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8821 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8881 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8941 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     9001 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     9061 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     9121 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     9181 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     9241 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     9301 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     9361 ggaaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     9421 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9481 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9541 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9601 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9661 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9721 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9781 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9841 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9901 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9961 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
    10021 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
    10081 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
    10141 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    10201 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    10261 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    10321 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    10381 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    10441 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10501 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10561 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10621 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10681 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10741 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10801 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10861 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10921 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10981 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    11041 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    11101 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    11161 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    11221 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    11281 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    11341 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    11401 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    11461 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11521 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11581 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11641 tgttcgcggg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11701 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11761 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11821 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11881 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11941 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    12001 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    12061 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    12121 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    12181 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    12241 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    12301 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    12361 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    12421 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12481 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12541 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12601 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12661 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12721 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12781 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12841 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12901 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12961 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    13021 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    13081 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    13141 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    13201 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    13261 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    13321 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    13381 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    13441 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13501 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13561 cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg
    13621 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13681 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13741 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13801 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13861 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13921 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13981 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    14041 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    14101 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    14161 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    14221 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    14281 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    14341 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    14401 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    14461 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14521 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14581 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14641 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
    14701 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
    14761 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
    14821 gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt
    14881 attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg
    14941 atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat
    15001 cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg
    15061 gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag
    15121 caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt
    15181 cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag
    15241 gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag
    15301 ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggca
    15361 ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag
    15421 acccggacgg cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc
    15481 gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt
    15541 atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa
    15601 ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt
    15661 tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt
    15721 gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat
    15781 cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt
    15841 attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga
    15901 cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt
    15961 ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag
    16021 gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt
    16081 gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca
    16141 acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt
    16201 attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc
    16261 aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag
    16321 gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa
    16381 ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg
    16441 atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc
    16501 cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact
    16561 gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct
    16621 ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg
    16681 ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc
    16741 atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg
    16801 catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt
    16861 ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc
    16921 ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg
    16981 attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt
    17041 gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt
    17101 tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc
    17161 gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac
    17221 ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc
    17281 ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt
    17341 ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac
    17401 gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca
    17461 cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt
    17521 ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg
    17581 gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct
    17641 gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc
    17701 gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc
    17761 atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg
    17821 gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt
    17881 tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt
    17941 attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt
    18001 actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcccc
    18061 cttaacaa
//
LOCUS       DAZSNG010000078        18046 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZSNG010000078 DAZSNG010000000
VERSION     DAZSNG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.079"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1089
                     /locus_tag="ACHW4Z_002788"
                     /pseudo
     CDS             1..1089
                     /locus_tag="ACHW4Z_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1235..2272)
                     /gene="iap"
                     /locus_tag="ACHW4Z_002789"
     CDS             complement(1235..2272)
                     /gene="iap"
                     /locus_tag="ACHW4Z_002789"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR8882358.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2524..3432
                     /gene="cysD"
                     /locus_tag="ACHW4Z_002790"
     CDS             2524..3432
                     /gene="cysD"
                     /locus_tag="ACHW4Z_002790"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR8882359.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3434..4861
                     /gene="cysN"
                     /locus_tag="ACHW4Z_002791"
     CDS             3434..4861
                     /gene="cysN"
                     /locus_tag="ACHW4Z_002791"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR8882360.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4861..5466
                     /gene="cysC"
                     /locus_tag="ACHW4Z_002792"
     CDS             4861..5466
                     /gene="cysC"
                     /locus_tag="ACHW4Z_002792"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR8882361.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5516..5839
                     /locus_tag="ACHW4Z_002793"
     CDS             5516..5839
                     /locus_tag="ACHW4Z_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR8882362.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6033..6344
                     /gene="ftsB"
                     /locus_tag="ACHW4Z_002794"
     CDS             6033..6344
                     /gene="ftsB"
                     /locus_tag="ACHW4Z_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR8882363.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6363..7073
                     /gene="ispD"
                     /locus_tag="ACHW4Z_002795"
     CDS             6363..7073
                     /gene="ispD"
                     /locus_tag="ACHW4Z_002795"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR8882364.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7073..7552
                     /gene="ispF"
                     /locus_tag="ACHW4Z_002796"
     CDS             7073..7552
                     /gene="ispF"
                     /locus_tag="ACHW4Z_002796"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR8882365.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7549..8598
                     /gene="truD"
                     /locus_tag="ACHW4Z_002797"
     CDS             7549..8598
                     /gene="truD"
                     /locus_tag="ACHW4Z_002797"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR8882366.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8579..9340
                     /gene="surE"
                     /locus_tag="ACHW4Z_002798"
     CDS             8579..9340
                     /gene="surE"
                     /locus_tag="ACHW4Z_002798"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR8882367.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9334..9960
                     /gene="pcm"
                     /locus_tag="ACHW4Z_002799"
     CDS             9334..9960
                     /gene="pcm"
                     /locus_tag="ACHW4Z_002799"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR8882368.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10145..11239
                     /gene="nlpD"
                     /locus_tag="ACHW4Z_002800"
     CDS             10145..11239
                     /gene="nlpD"
                     /locus_tag="ACHW4Z_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR8882369.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11302..12294
                     /gene="rpoS"
                     /locus_tag="ACHW4Z_002801"
     CDS             11302..12294
                     /gene="rpoS"
                     /locus_tag="ACHW4Z_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR8882370.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12414..12821)
                     /locus_tag="ACHW4Z_002802"
     CDS             complement(12414..12821)
                     /locus_tag="ACHW4Z_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR8882371.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12968..13561
                     /locus_tag="ACHW4Z_002803"
     CDS             12968..13561
                     /locus_tag="ACHW4Z_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR8882372.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13561..14508
                     /locus_tag="ACHW4Z_002804"
                     /pseudo
     CDS             13561..14508
                     /locus_tag="ACHW4Z_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14781..15344)
                     /locus_tag="ACHW4Z_002805"
     CDS             complement(14781..15344)
                     /locus_tag="ACHW4Z_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882373.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15509..15760
                     /locus_tag="ACHW4Z_002806"
     CDS             15509..15760
                     /locus_tag="ACHW4Z_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8882374.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15762..17858
                     /locus_tag="ACHW4Z_002807"
     CDS             15762..17858
                     /locus_tag="ACHW4Z_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR8882375.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4457 a         4405 c         4838 g         4346 t
ORIGIN      
        1 cgtaaatatc ctttaagttt actgaaggat aaaaatattg tgactttctt tgatttctgg
       61 ggaaaaaacc gacgtggcga gaaagagggt atcgacgact atcaccttct ttgctggcat
      121 ttgctggatg tggccgcaat gggctattta atggttaaaa gaaattgctt cgggctggct
      181 gattattttc gtcaattagg gatttctgac aaggaacagg cagctcgatt tttcgcctgg
      241 ttgctgtgct ggcacgatat tggaaaattt gcccgctctt ttcaacaact ttacctgccc
      301 cctgaattca agattcagga aggtgccaga aaaaattacg aaaaaatctc tcattcaacg
      361 ctgggctact ggttgtggaa tcattattta agtgaatgtc aggagttact tccttcatct
      421 tcgctctctc cgcgcaaact taggcgagtg atagaaatgt ggatgcccgt gacgacaggg
      481 catcatggtc ggccccctga tcgtatggat gaattggata attttctgcc tgaagacaaa
      541 gctgcagcac gagattttct ccttgaaatc aagccactgt ttccgcgaat agagatcccc
      601 acattctggg atgatgacga gggcgttgaa cttataaaac aactttcctg gtatatctct
      661 gcaaccgtcg tacttgcaga ctggacaggg tcgtcaacgc gattttttcc acgcgtcgca
      721 caggcaatgg atattgaaga ttactggcaa aaggctttag ttcaggctca aaacgcatta
      781 accgtctttc cgccacaagc agaaaccgcg cctttcaccg gaattaatac gctgtttcct
      841 tttattgaga atacgacacc gttacagcaa aaggtgctgg atctggatat caatcagcca
      901 ggaccacagt tgtttattct ggaagatgtg accggcgcag gtaaaacaga agcggcgctt
      961 atcctggcgc acaggttgat ggctgcgggt aacgctcagg ggctgttttt tggcctgcca
     1021 acaatggcaa cggccaatgc catgtacgat cgactggtca aaacctggct tgctttctat
     1081 tcgccagagg tgttccccgc tccagcggag ataaaccggc gcaatacggt gcggttttcc
     1141 cggaagtaaa ccggacgcac tggatgcgat gatgggtatc acttagaatt ccccgcccct
     1201 gcggtagaac tcccagctcc cattttcaaa cccatcaaga cgccttcgcc agctccttca
     1261 ccaacggtag cattatccgc ataacgtcac ggcagcgacg ttctattctt ccaggaagtg
     1321 ccttatcaat atgttgttga ttatccagtc ttacgtcatg ccagctattt cccgccggga
     1381 aggcaggtgt ttttgcgcgt tgctgataac catccttatt tccaagattc cagttagtcg
     1441 cttccaccga aagtacagca atgcccgctt tgtcgaatat ttctgcgtca ttacaacccc
     1501 cagtgccttt cggataattt ttattcaaac ccggattggt cgttgcggct attccgtgac
     1561 tgcgtgcaat tgccagcgcc ctgtcgcgcg ttaatttcct tactgcttca ggggttttta
     1621 caccgctgtt gaaatacaat ttatcgccaa caattaagtt atcgagatta atcaccagca
     1681 gcgtattttt cttttcggtg tcactcatcc gcttgagtaa attctcagcg cctaattttc
     1741 cttcctcttc gccgctggtc gccacaaatc gaataccata ctcggtaggc gtatttttca
     1801 ggcgttctgc cagttccagc atgacaccta aacctgcggc gttatcatct attccttgta
     1861 acgtcagtcc gccgagattg gcatctgcgt cagcatcgct cagcggggcg taagtatcca
     1921 gatgcgccat aatgatgatc tgctgcggcg ctttgccttc atgagcggca atcaccgtac
     1981 ttcctgtcac gttatgccag ttcttgcgat tatcgcgggc ggtataaata taccgactat
     2041 taaatgtccg aatatcactg cgataaccca tttgctgaaa ctgttggcga atataatcgg
     2101 cagataacat ttctgctgga gttccggtca tgcgtcccgg aaagaaagta gcaatatgtc
     2161 gtgcctgggt attagcaaaa tcgccaggtt taggtgacga ggcgtgtacg gggagaataa
     2221 agcatacgcc gagcgccagg gcagcggtac ggtggcgcaa tgcggaaaac atagtgagtc
     2281 cttaaatacc atgcaaattt ttttaccgcc atagtatgaa actgccgctg cgctaaaaca
     2341 atttcaaatc ttcctaaacg cccgaaatcc ggtgccttaa gcactttttg atattagctt
     2401 tgccaaatcg ttattccgtt aaggaactac tcattctaat tggtaatttc attcgttctc
     2461 ttacgctccc tatagtcgaa acatctgatg gcaagaaaat agcggtattg caaaggaacg
     2521 gttatggctc aaaaacgact tactcacctg cggcaactgg aggcggaaag catccacatt
     2581 attcgcgagg tggcggcaga attctcaaat ccggtgatgc tctactctat cggtaaagat
     2641 tccagcgtca tgctgcatct ggcgcgcaag gcgttttatc caggtacgct gcctttcccg
     2701 ttgctgcatg tcgataccgg ctggaaattc cgcgagatgt atgagttccg cgatcgtacg
     2761 gcgaaagcct acggctgcga gctgctggtg cataaaaacc cggaaggcgt ggcgatgggg
     2821 attaatccat tcgtgcacgg cagcgcgaaa cataccgata ttatgaaaac tgaaggcctg
     2881 aaacaggcgc tgaacaaata cggttttgat gccgccttcg gtggtgcgcg ccgtgacgaa
     2941 gagaaatccc gcgctaaaga gcgaatctac tctttccgtg accgcttcca tcgctgggat
     3001 ccgaaaaatc agcgcccgga gctgtggcac aactacaacg ggcaaattaa caaaggcgaa
     3061 agcatccgcg tcttcccgct ctctaactgg accgagcagg atatctggca atacatctgg
     3121 ctggaaaata tcgacattgt gccgctgtat ctcgctgcgg aacgtccggt tctggaacgc
     3181 gacggtatgt tgatgatgat tgatgacaac cgtatcgacc tgcaaccggg cgaagtgatc
     3241 aaaaaacgga tggtgcgttt ccgtacgctg ggctgctggc cgctgaccgg tgcggtggag
     3301 tcaaatgcac aaacactgcc ggaaatcatc gaagagatgc tggtttccac caccagtgaa
     3361 cgtcagggtc gcgtgattga ccgcgaccag gcgggatcca tggagctgaa aaaacgtcag
     3421 gggtattttt aagatgaaca ccgtacttgc acaacaaatc gccaatgaag gcggcgtcga
     3481 agcctggatg attgcgcaac aacataaaag cctgctgcgt tttctgacct gtggtagcgt
     3541 cgatgacggc aaaagtaccc tgattggtcg tctgctgcac gatacccgcc aaatctacga
     3601 agatcagctc tcatcgctgc ataacgacag taagcgtcac ggaacccagg gcgaaaagct
     3661 ggatctggct ctgctggtgg acggcctgca agctgagcgc gaacagggca tcactattga
     3721 tgtggcctac cgctatttct ctaccgagaa gcgtaaattt attatcgccg acaccccagg
     3781 gcacgagcag tacactcgca atatggcgac tggcgcatcg acatgtgaac tggcgatctt
     3841 actgattgat gcccgtaaag gcgtgctcga tcaaacccgt cgtcacagtt ttatctccac
     3901 actgttgggg atcaaacatc tggtcgtggc gatcaacaaa atggatctgg tggattacag
     3961 tgaagagacg ttcacccgta ttcgtgaaga ttatctgacc tttgccgggc agctgccggg
     4021 taatctggat atccgctttg tgccgctctc cgcactggaa ggtgacaacg tggcttcgca
     4081 aagtgaaagt atgccgtggt acagcggtct gacactgctc gaagtgctgg aaaccgtgga
     4141 gatccagcga gtggtggatg cccagccaat gcgcttcccg gtgcagtacg ttaaccgccc
     4201 aaatctcgat tttcgtggct acgccggaat gctggcatcc ggtcgcgtgg aagtcgggca
     4261 acgtgtaaaa gtgctgccct ctggtgtgga atcaaacgtc gcgcggatcg tgacttttga
     4321 tggtgatcgc gaagaagtct ttgccggaga agcgatcacc ctggtgctga cggatgagat
     4381 cgatatcagc cgtggtgatc tgctgctggc ggcagacgaa gcgttaccgg cggtgcagag
     4441 tgcgtcggtg gatgtggtat ggatggcgga acagccgctt tccccgggcc agagttatga
     4501 catcaaaatt gccggtaaga agacgcgtgc tcgtgttgat ggcattcgtt atcaggttga
     4561 tattaataac cttacccaac gcgaagttga aaacctgccg ctgaacggca tcggcctggt
     4621 ggatctcact tttgacgagc cactggtgtt agatcgctat cagcaaaatc cggttaccgg
     4681 tgggctgatt tttatcgatc gcctgagcaa tgtgaccgtg ggtgccggta tggtgcacga
     4741 gccagttagc caggcaacag ctgcgtcatc tgaattcagt gcattcgaac tggaattgaa
     4801 tgccctggtt cgtcgccact ttccgcactg gggcgcgcgc gatttgctgg gggataaata
     4861 atggcgctgc atgacgaaaa cgtcgtctgg catagtcatc cggtcactgt gcaacaacgc
     4921 gaactacacc acggtcatcg tggtgtagtg ctgtggttta ccggcctctc cgggtccggt
     4981 aaatcaacgg tcgccggggc gctggaggag gcgttacata aactcagcgt cagtacgtat
     5041 ctgctggatg gcgacaatgt tcgccacgga ttatgcagcg atctcggttt tagcgatgcc
     5101 gatcgtaaag agaatatccg tcgcgtcggt gaagtggcga atttgatggt tgaagccgga
     5161 ctggtggtgc tgaccgcatt tatctcgcca caccgcgccg aacgccagat ggttcgcgaa
     5221 cgcgtaggag aagggcgctt tattgaagtg tttgtcgata cgccgctggc gatttgcgaa
     5281 gcccgcgatc caaaaggctt atataagaaa gcgcgtgccg gtgaactgcg caactttacg
     5341 ggaatagatt ccgtttacga agcgcctgaa tcggcagaaa ttcatctcaa tggtgaacaa
     5401 ttagtaacaa atttggtaca gcaattatta gatctgttga gacagaacga tattatcaga
     5461 tcctgagaca ccaccgggtt tccatgcccg gttcgtcaaa gtaacaggat caaatatgcg
     5521 taatagccat aacattacac taacaaataa cgacagcctt actgaggatg aagaaactac
     5581 atggtcactg cctggtgccg tggtcggttt tatctcctgg ttatttgcgc tggcgatgcc
     5641 gatgttgatt tatggctcta acacgctgtt cttctttctc tacacctggc ctttctttct
     5701 ggcactgatg cctgtcgcgg tagtggtggg gattgcgctg cattcattga tggacggaaa
     5761 gctacgctac agtattgttt tcaccctggt gacagtaggc attatgtttg gcgcactgtt
     5821 tatgtggcta ctgggctaat ttgtactttc ttgtctcctc tgttcataat tcaaaccgta
     5881 actaataatg agattatgtt ctgcacgccc tgggtatacg taacaatggg caaatgtggt
     5941 acatttgccc gcgttgtcgc ggtatcccca acagaggatg tagagtcgtc ttcggatgca
     6001 tgggatgatg atgcagtttt tcagggggca ggatgggtaa actaacgctg ctgttgctgg
     6061 ctattctggt ctggctacag tattcgctgt ggttcggtaa gaacggtata catgactata
     6121 cccgcgtcaa tgatgatgtg gcggcactgc aagctacaaa cgcgaaactt aaagcgcgaa
     6181 acgatcaact ttttgccgaa attgacgatc tcaatggcgg ccaggaggcg ctcgaagagc
     6241 gtgcgcgtaa tgaactcagc atgaccaggc cgggcgaaac tttttatcgt ctggtgcctg
     6301 acgcgtcgaa gcgcgcacag tctgcggggc aaaacaatcg ataaatcagc ccgggaatta
     6361 acatggcaac cactcatttg gatgtttgcg ccgtggttcc ggcggccgga tttggccgtc
     6421 gaatgcaaac ggaatgtcct aagcaatatc tctcaatcgg taatcaaacc attcttgaac
     6481 actcggtgca tgcgctgctg gcgcatcccc gggtgacacg tgtcgtcatt gccataagtc
     6541 ctggcgatag ccgttttgca caacttcctc tggcgaatca tccgcaaatc accgttgtag
     6601 atggcggtga tgagcgtgcc gattccgtgc tggcaggtct gaaagccgct ggcgatgtgc
     6661 agtgggtatt ggtgcatgac gccgctcgtc cttgtttgca tcaggatgac cttgcgcgat
     6721 tgttggcgtt gagcgaaacc agccgcacgg gagggatcct cgccgcacca gtgcgcgata
     6781 ctatgaaacg tgccgaaccg ggcaaaaatg ccattgctca taccgttgat cgcaacggct
     6841 tatggcacgc gctgacgccg caatttttcc ctcgtgagct gttacatgac tgtctgacgc
     6901 gcgctctaaa tgaaggcgct accattaccg acgaagcctc ggcgctggaa tattgcggat
     6961 ttcatccgca gttggtcgaa ggccgcgcgg ataacattaa agtcacgcgc ccggaagatt
     7021 tggcactggc cgagttttac ctcacccgaa ccatccatca ggagaataca taatgcgaat
     7081 tggacacggt tttgacgtac acgcctttgg cggtgaaggc ccaattatca ttggtggcgt
     7141 acgcattcct tacgaaaaag gattgctggc gcattctgat ggcgacgtgg cgcttcatgc
     7201 gttgaccgat gcattgcttg gcgcggcggc gctgggggat atcggcaaac tgttcccgga
     7261 taccgatccg gcatttaaag gtgccgacag ccgcgagctg ctacgcgaag cctggcgtcg
     7321 tattcaggcg aagggttata cccttggcaa cgtcgatgtc actatcatcg ctcaggcacc
     7381 gaagatgttg ccgcacattc cacaaatgcg cgtgtttatt gccgaagatc tcggctgcca
     7441 tatggatgat gttaacgtga aagccactac tacggaaaaa cttggattta ccggacgtgg
     7501 ggaagggatt gcctgtgaag cggtggcgct actcattaag gcaacaaaat gattgagttt
     7561 gataatctca cttacctcca cggtaaaccg caaggcaccg ggctgctgaa agccaatccg
     7621 gaagactttg tggtggtgga agatttgggc tttgagcctg atggtgaagg tgagcatatt
     7681 ctggttagaa tcctcaaaaa cggctgcaat acccgttttg tggcggatgc actggcgaaa
     7741 ttcctgaaaa ttcattcccg tgaagtcagc ttcgctgggc aaaaagacaa acatgctgta
     7801 acggaacagt ggttatgtgc tcgcgtgccg ggcaaggaaa tgcccgatct gagcgccttt
     7861 caactggaag gctgccaggt gctggagtat gcgcggcaca agcgcaagct gcgtttaggg
     7921 gcgctgaaag gtaacgcctt taccctggtt ctgcgcgaag tgagcaatcg cgatgacgtt
     7981 gaacaacgtc tgatcgatat ttgcgtaaaa ggtgtaccga actacttcgg tgcccaacgt
     8041 tttgggattg gcggtagcaa cttgcagggc gcgctgcgct gggcgcaaac caatactccg
     8101 gtgcgcgatc gcaataaacg gagtttttgg ttgtcggcag cccgcagtgc gttgtttaat
     8161 cagattgttg ctgagcgcct caaaaaagca gacgttaatc aagttgttga cggcgatgcg
     8221 ctacaattag ccggacgtgg tagctggttt gtagcaacca ccgaagaact ggcggaatta
     8281 cagcgtcgcg tcaacgataa agagctgatg ataaccgccg cattgccggg cagtggcgaa
     8341 tggggaactc agcgtgaagc gctggcattc gaacaagcag ctgtcgccgc agaaactgaa
     8401 ttacaagctt tactggtgcg cgaaaaagtt gaagccgcgc gcagagcgat gctgctgtat
     8461 ccgcaacaat taagctggaa ctggtgggat gacgtcaccg tagagatccg tttctggctt
     8521 ccggcgggta gttttgcaac cagcgttgtc agggaactta tcaacacaac aggtgattat
     8581 gcgcatattg ctgagtaatt atgacggggt acatgcaccc ggtatacaaa cgctggcgaa
     8641 agccttgcgt gagtttgctg acgttcaggt ggtcgccccc gatcgtaacc gcagcggcgc
     8701 ttcaaattct ctgacactgg aatcctccct gcgcacgttt acctttgaaa atggtgatat
     8761 tgctgtgcaa atgggaaccc cgaccgattg tgtctatctt ggcgtgaatg ctctgatgcg
     8821 tccgcgcccg gacattgttg tgtccggaat taacgccggg ccgaatctgg gggatgatgt
     8881 tatttattcc ggtacggtag ccgccgcgat ggaaggccgt catttaggtt ttccggcgct
     8941 ggccgtctcg cttgacgggc ataaacatta cgacactgcc gcggcggtaa cctgttcaat
     9001 tttgcgcgca ctgtgtaaag agccgctgcg caccggacgt attcttaata ttaacgttca
     9061 ggatttaccc ttggatcaaa tcaaaggtat tcgcgtgacg cgctgcggta cacgacatcc
     9121 ggcagatcag gtgatcccgc agcaagatcc gcgcggcaat acgctgtact ggattggccc
     9181 gccgggcggt aaatgtgatg ccggtccggg gaccgatttt gctgcggtag atgagggcta
     9241 tgtctccatc acgccgctgc atgtggattt aactgcgcat agcgcgcaag atgtggtttc
     9301 agactggtta aacagcgtgg gagttggcac gcaatggtaa gcagacgcgt acaagcactt
     9361 ctggatcaat tacgtgcgca aggtattcag gatgagcagg tgctgaatgc acttgccgcc
     9421 gtgccgcgtg aaaaattcgt tgatgaagcg tttgaacaaa aagcctggga caatatcgcc
     9481 ttgccgatag gtcaggggca gacaatttcg cagccgtata tggtggcgcg aatgaccgaa
     9541 ttactcgagc tgacgccgca gtcgcgggtg ctggaaattg gcactggttc gggatatcaa
     9601 acggcaatcc tggcgcatct tgtccagcat gtttgctcgg ttgaacggat taaaggcttg
     9661 cagtggcagg cacgtcgccg cctgaaaaat cttgatttac ataatgtttc aacccgtcat
     9721 ggtgatggat ggcaaggttg gcaggcacgt gcgccgtttg acgctatcat tgttacggcg
     9781 gcaccgccgg aaattccaac tgcgctaatg acgcagctgg acgaaggcgg gattctcgtc
     9841 ttacccgtag gggaggagca ccagtatttg aaacgggtgc gtcgtcgggg aggcgaattt
     9901 attatcgata ccgtggaggc cgtgcgcttt gtccctttag tgaagggtga gctggcttaa
     9961 aacgtgagga aatacctgga tttttcctgg ttattttgcc gtaggtcagc gtatcgtgaa
    10021 catcttttcc agtgttcagt agggtgcctt gcacggtaat tatgtcactg gttattaacc
    10081 aatttttcct gggggataaa tgagcgcggg aagcccaaaa ttcaccgttc gccgcattgc
    10141 ggctttgtca ctggtttcgc tatggctggc aggctgttct gacacttcaa atccaccggc
    10201 accggtcagc tccgttaatg gcaatgcgcc tgcaaatact aattctggta tgttgattac
    10261 gccgccgccg aaaatgggga cgacgtctac agcgcagcaa ccgcaaattc agccggtaca
    10321 gcagccacaa attcaggcta ctcaacaacc gcaaatccag ccagtgcagc cagtagctca
    10381 gcagccggta cagatggaaa acggacgcat cgtctataac cgtcagtatg ggaacattcc
    10441 gaaaggcagt tatagcggca gtacctatac cgtgaaaaaa ggcgacacac ttttctatat
    10501 cgcctggatt actggcaacg atttccgtga ccttgctcag cgcaacaata ttcaggcacc
    10561 atacgcgctg aacgttggtc agaccttgca ggtgggtaat gcttccggta cgccaatcac
    10621 tggcggaaat gccattaccc aggccgacgc agcagagcaa ggagttgtga tcaagcctgc
    10681 acaaaattcc accgttgctg ttgcgtcgca accgacaatt acgtattctg agtcttcggg
    10741 tgaacagagt gctaacaaaa tgttgccgaa caacaagcca actgcgacca cggtcacagc
    10801 gcctgtaacg gtaccaacag caagcacaac cgagccgact gtcagcagta catcaaccag
    10861 tacgcctatc tccacctggc gctggccgac tgagggcaaa gtgatcgaaa cctttggcgc
    10921 ttctgagggg ggcaacaagg ggattgatat cgcaggcagc aaaggacagg caattatcgc
    10981 gaccgcagat ggccgcgttg tttatgctgg taacgcgctg cgcggctacg gtaatctgat
    11041 tatcatcaaa cataatgatg attacctgag tgcctacgcc cataacgaca caatgctggt
    11101 ccgggaacaa caagaagtga aggcggggca aaaaatagca accatgggta gcaccggaac
    11161 cagttcaaca cgcttgcatt ttgaaattcg ttacaagggg aaatccgtaa acccgctgcg
    11221 ttatttgccg cagcgataaa tcggcggaac caggcttttg cttgaatgtt ccgtcaaggg
    11281 atcacgggta ggagccacct tatgagtcag aatacgctga aagttcatga tttaaatgaa
    11341 gatgcggaat ttgatgagaa cggagttgag gtttttgacg aaaaggcctt agtagaagag
    11401 gaacccagtg ataacgattt ggccgaagag gaactgttat cgcagggagc cacacagcgt
    11461 gtgttggacg cgactcagct ttaccttggt gagattggtt attcaccact gttaacggcc
    11521 gaagaagaag tttattttgc gcgtcgcgca ctgcgtggag atgtcgcctc tcgccgccgg
    11581 atgatcgaga gtaacttgcg tctggtggta aaaattgccc gccgttatgg caatcgtggt
    11641 ctggcgttgc tggaccttat cgaagagggc aacttggggc tgatccgcgc ggtagagaag
    11701 tttgacccgg aacgtggttt ccgcttctca acatacgcaa cctggtggat tcgccagacg
    11761 attgaacggg cgattatgaa ccaaacccgt actattcgtt tgccgattca catcgtaaag
    11821 gagctgaacg tttacctgcg aaccgcacgt gagttgtccc ataagctgga ccatgaacca
    11881 agtgcggaag agatcgcaga gcaactggat aagccagttg atgacgtcag ccgtatgctt
    11941 cgtcttaacg agcgcattac ctcggtagac accccgctgg gtggtgattc cgaaaaagcg
    12001 ttgctggaca tcctggccga tgaaaaagag aacggtccgg aagataccac gcaagatgac
    12061 gatatgaagc agagcatcgt caaatggctg ttcgagctga acgccaaaca gcgtgaagta
    12121 ctggcacgtc gattcggttt gctggggtat gaagcggcaa cactggaaga tgtaggtcgt
    12181 gaaattggcc tcacccgtga acgtgttcgc cagattcagg ttgaaggcct gcgccgtttg
    12241 cgcgaaatcc tgcaaacgca ggggctgaat atcgaagcgc tgttccgcaa gtaagtaagc
    12301 atctgtcaga aaggccagtc tcaagcgagg ctggcctttt tcttttgttt gggaagcttg
    12361 tgtttttgtc gcctggataa gacacgtcag cgtcctaccc ggcaatcagc attctatgtg
    12421 ctcatcatct tgcgcaccaa ctgcataaac agctcctgct cttctccact caaacgcccc
    12481 aaaaattcct catccacgct gtcaccaata ggtatcgccg cagcaagtag cttttctcct
    12541 tcggcagtca gccagacaaa gcgtcgccgc ttgtcagcga cgtcatgttc acgtctgact
    12601 aagccacgat tttccattct tgccaacatt tctgctagcg ttgctttggt gctgactgct
    12661 gcttctatca gtgccacctg ttcaatacca ggtttatcag caatggcgcg catcaccgcg
    12721 tactgtggtt tggtcaggtc aggtaactcg tgctgccagc gagccgtgtg ctgctgaaaa
    12781 agctgtcgta actgatggaa cgctttattt cgtaacgcca tgtaaactcc gggctaattc
    12841 tctgtcgcta tcatagcggt tttttagtga cgaagaagat gttgttactt ttcaatcctt
    12901 gccgctgtgg aaatgaagga gtatgttaat aataacgttc gtatacgaat aattaagaga
    12961 ataagcaatg aaactgatcg tcgggatgac aggggctacc ggtgcgcctc ttggtgtggc
    13021 attactgcaa gcgctgcgtg agatgccgaa tgtcgagact catctggtga tgtcgaagtg
    13081 ggcgaaaacc accattgaac tggaaacgcc ttacagcgct cgcgatgttg ctgccctcgc
    13141 agacttcagc cataacccgg cggatcaggc ggcgatcatc tcatccggtt cttttcgtac
    13201 cgacggcatg atcgttattc cgtgcagtat gaaaacgctc gccggtatcc gcgctggtta
    13261 cgccgatggc ctggtagggc gcgcggcgga cgtcgtgctc aaagaaggcc gcaaactggt
    13321 gctggtgccg cgtgaaatgc cgcttagcac catccatctc gaaaatatgc tcgcactttc
    13381 acgcatgggc gtggcgatgg tgccgccgat gcctgccttt tataaccatc ccgaaacggt
    13441 agatgacatt gtccaccatg tggtagcccg cgtgctggat caatttggcc tcgaacatcc
    13501 ccacgccagg cgctggcaag gattgccgca ggcccggaat ttttctcagg agaatgaata
    13561 atggcatttg atgatttacg cagcttttta caggcgcttg atgaccacgg ccagttactg
    13621 aaaatcagcg aagaagtgaa cgctgagccg gatctggcag cagcagctaa cgccaccggg
    13681 cgtatcggcg acggcgcgcc cgcgctgtgg tttgataata ttcgcggctt taccgatgcc
    13741 cgcgtggcga tgaataccat cggttcctgg cagaaccacg cgatttccct tggcctgcca
    13801 ccaaacaccc cggtcaaaaa gcagattgat gagtttatcc gccgctggga taacttcccg
    13861 attgccccgg agcgccgcgc caatccagcc tgggcgcaga acaccgtcga tggcgaagag
    13921 atcaacctgt tcgatattct gccgctgttt cgcctgaatg atggcgatgg cggtttctat
    13981 ctcgacaaag cgtgcgtggt ttcccgcgat ccgctcgacc cggataactt cggcaagcag
    14041 aacgtcggca tctaccgcat ggaagtgaag ggcaagcgta agctcggcct gcaaccggtg
    14101 ccgatgcacg atatcgccct gcatctgcat aaagcagaag agcgcggtga agatctgccg
    14161 attgcgatca cgctcggtaa cgatccgatc atcacgctga tgggggccac gccgctgaaa
    14221 tacgatcagt ctgagtatga aatggcaggc gcgctgcgtg aaagcccgta cccgatcgcc
    14281 accgctccgc tgaccggttt tgatgtgccg tggggttcag aagtgatcct cgaaggggtt
    14341 atcgaaagcc gtaaacgcga aatcgaaggg ccgttcggtg agtttaccgg gcactactcc
    14401 ggtgggcgca acatgaccgt ggtgcgcatc gataaagtct cttatcgcag caaaccgatt
    14461 ttcgaatcac tctatctcgg tatgccgtgg accgaaatcg actacctatg actaggtaat
    14521 ttattcaaga taaccgggaa gagattaaaa aagacgggaa tagattggaa tcaacaagtt
    14581 aggaactcaa ggttttcaag ataaagcgag tccaaaaatg ttaaacggtt cgatatcaat
    14641 aaaacattca agataactca caggttctta caatagcctt taaagaccgt tcaaaaacga
    14701 ttcaggtgtt tattatagcg ctgatgggtg caagcgccaa aaccaatgta attttagcac
    14761 agctaggcac ttgcactggg ttatgcgctg gctgctttct gaggtagatt taagtgatcg
    14821 cccttagctt cggacaacgc gtaaatctct ctaacctttt cgtctggcaa attaccaagg
    14881 cttaacgcta tggctctacg aactccaact agagcgtgca actcttggat gtgctcatcc
    14941 agaagaatga gtaattgctc cgctcctttt ctcccaagaa catcagctac ttgttgtttt
    15001 tcttgtggtt taagaaatga caagaacatt agaatttgag catcaacatt atccgcattc
    15061 tcttttttgg gttctttgga atcttttagt tcaacatcac cctctcccgt catgagccat
    15121 tccaaggtaa cattttcgct ttctgctatc ttttttaact gatacgggcg tggtatcggt
    15181 gcgtaatccc gcctgttgta ataatttttt aaagtgcctt cattgatccc ccatgcccga
    15241 gcagccgcat ttcttgaagg gtatcgttct actaacctaa agattctttc gataatcggt
    15301 tcttttccat cggcaggaaa agaaccttgg ggtgcttttc tcattcttgg ttcctttcta
    15361 tctaattcat tgataaataa taaaattaaa aaataaacca taaaaacacc aaagaaccct
    15421 ttgcagggaa tctttggtat cttaatcttt atccatacgg atatccgacc ggatatccgc
    15481 gcgggtaatt ttgtaggtta aaggaaagat gagacaaaga gaagatagtg accgcgattg
    15541 gccttcgctg aagattcagc tggcgctcaa agataacggg tggtcttcct tccgggagct
    15601 ttcactccat gctggttatg ctcaggattc actgaaagcg gtagttcgca caccaaacaa
    15661 aaaatacgaa cgcgtaattt ctgacgctat aggtgtaccg ccagaaatca tctggcccag
    15721 ccggtatcgt aacgatggat ttatcaggaa ggcgtcgtga tatgcagtcg tacgttaccg
    15781 taaatgacct gctcggcgta ccaggaatgc cagccaccac aaaaggcatc cgacaggcgt
    15841 tacagcgttt ttctcgtgat cttggtgacg ttagtcgccg tcgggaaggc acaaaagcca
    15901 ttgagtacca cattgattgt ctgcctgaaa taacacgaaa ggcactgcgt gaacgctatg
    15961 tggaacagct ggtggctaca gagaacaatg tttctgaagt gaaagctgtt accagaaaaa
    16021 cacgcaatcc tgacgctgtc caggcaatcg aagcatatcg cggttctcca caactgatgg
    16081 aagaacgcct gaatgcgctg accgaaaacc agcgctgggt atctgaagca agagctgcgc
    16141 tggtggtgga agtgctgaag ctggaaagcg ccggtaaccc cgggcgactg aaagccatta
    16201 actttcttgt tgaaaaagcc cgtaaaggtg agctgccgga gcgcctgcaa caggccgcag
    16261 ttaacgccaa tgcaaaacgt ggcgctaatc gtactatcag ccgcgatcca ctttaccagt
    16321 gggttctgaa atacaaccag tcgcagaacg ccgccgaacg gctgctgtta ctggcaccgg
    16381 gaaaacgcga cgaaataaaa ccggaagaaa tcagctggct gcctgaattt ctggcgcagt
    16441 atcgccaggt gaacggcaga cccatgtcag aagcctacga ggattttgtc gcagaatggc
    16501 agcgtcgcca tgcggacgaa ccgtacatgc tggaggtgat gccttcctat gatgttgtcc
    16561 ggtatgccat gaaaaaactg ccggaagtgg tgaaacaaaa agggcgcgtt accggcagtg
    16621 aataccgtca gcttgaagga ttcacccgcc gcgactggac agctatgccg gtgaattatg
    16681 tctggattgg tgacggtcac ggtatgaagc tgaaatgtgc gcatccgatc cacgggcgtc
    16741 cattctcacc ggaagtcacg tttgtgatcg atggtggaac acggtttgtt gttggctgga
    16801 gtcttgacct tgccgaaaac gtgttcgccg ttgccggtgc gatacagcac ggcattcgca
    16861 accacggaaa accgttcctg tattactcgg ataacggttc cggtgaaacg gctgacatgc
    16921 tggacaaaga ggtcgtgggt attctgccac gcctggggat taaacaccct accggtattg
    16981 ccggtaatcc acagggacgc ggcattattg aacgcctgaa ccggacactg ccgatgcgta
    17041 tagccaggag gtatcgcacc tattttggca agggtgctga ccgggaatcg ttgcgtgtac
    17101 ttaaccgtga tctgcgttca gcgtttaacg ccctgcaaca ggacaagcct ctgaacgacc
    17161 gccagaaagc ggcaatgcgc gagctgccat cgtgggcgga gcttattgag gctattcgtg
    17221 aaggcgtgga atggtacaac aaccgcccgc attctgagct gccgatgaaa ccgaacggac
    17281 ggcattacag cccgacagag ttcagaaaaa aacgccaggc agaagaagac accgaaattg
    17341 agtggctgag tgatcttgag ctgcgcgaca tgttccgccc aatggtggaa cgaccggtaa
    17401 gacgctgcga aattcagtgg ctgaacaata tttactacgc gccggagttg cgtgacgaac
    17461 acggtcgcaa agtgcttatc agttatgaca ttcatgacgc cgagcgaatt acggtacgtc
    17521 gcaaggacgg cagctttatc tgtgaggcga tatggaacgg caataaacgc gcagcctttg
    17581 ctgtcagtgc tgaatatcac aaacaacagc agcgtattaa aggaatgcgc aaacgcgctg
    17641 aggaaaaaat tcgtgatgct gaagatgaag gtattcagat tctggaacac aaacaggctg
    17701 aaccctggct gagtaatgtt tatcgcccgg ttggtaatgt ggtggcagtt cagcaaccgg
    17761 aatatgaaga agagcatgac gaagaatttg aacgcgattt ccggctgggg atgcaaaaac
    17821 tttttgccat gcaggaagaa gatgatccgc tggcctgaat aaaaaaacag tccgcgggaa
    17881 cggactgtta aataaaggaa acaaagctga tttgaaataa ccgaggaaat actatgactg
    17941 atattgatga tgtttgaggt gtactggcaa tagcggacac taccatttgt tcttttttta
    18001 agcagccatc tgatgatatt tttccctgaa ggctgccggg gagata
//
LOCUS       DAZSNG010000079        17912 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZSNG010000079 DAZSNG010000000
VERSION     DAZSNG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17912
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.080"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(383..1756)
                     /gene="eutA"
                     /locus_tag="ACHW4Z_002808"
     CDS             complement(383..1756)
                     /gene="eutA"
                     /locus_tag="ACHW4Z_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR8882376.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(1731..2210)
                     /gene="eutP"
                     /locus_tag="ACHW4Z_002809"
     CDS             complement(1731..2210)
                     /gene="eutP"
                     /locus_tag="ACHW4Z_002809"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR8882377.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2223..2558)
                     /gene="eutS"
                     /locus_tag="ACHW4Z_002810"
     CDS             complement(2223..2558)
                     /gene="eutS"
                     /locus_tag="ACHW4Z_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR8882378.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(2851..5130)
                     /gene="maeB"
                     /locus_tag="ACHW4Z_002811"
     CDS             complement(2851..5130)
                     /gene="maeB"
                     /locus_tag="ACHW4Z_002811"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR8882379.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5419..6369
                     /gene="tal"
                     /locus_tag="ACHW4Z_002812"
     CDS             5419..6369
                     /gene="tal"
                     /locus_tag="ACHW4Z_002812"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8882380.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6366..8381
                     /gene="tkt"
                     /locus_tag="ACHW4Z_002813"
     CDS             6366..8381
                     /gene="tkt"
                     /locus_tag="ACHW4Z_002813"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8882381.1"
                     /translation="MNQPRSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLW
                     NDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPG
                     HPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGC
                     LMEGISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHE
                     IDGHDPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALA
                     RQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSG
                     GLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTI
                     WKGSVSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARM
                     AALMKARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKL
                     AVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEI
                     TLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYK
                     YVGLKGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8484..9527)
                     /locus_tag="ACHW4Z_002814"
     CDS             complement(8484..9527)
                     /locus_tag="ACHW4Z_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR8882382.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(9653..10228)
                     /gene="nudK"
                     /locus_tag="ACHW4Z_002815"
     CDS             complement(9653..10228)
                     /gene="nudK"
                     /locus_tag="ACHW4Z_002815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR8882383.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(10296..12275)
                     /gene="aegA"
                     /locus_tag="ACHW4Z_002816"
     CDS             complement(10296..12275)
                     /gene="aegA"
                     /locus_tag="ACHW4Z_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR8882384.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            12481..14198
                     /gene="narQ"
                     /locus_tag="ACHW4Z_002817"
                     /pseudo
     CDS             12481..14198
                     /gene="narQ"
                     /locus_tag="ACHW4Z_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            14400..17513
                     /gene="acrD"
                     /locus_tag="ACHW4Z_002818"
     CDS             14400..17513
                     /gene="acrD"
                     /locus_tag="ACHW4Z_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR8882385.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17612..17671)
                     /gene="ypfM"
                     /locus_tag="ACHW4Z_002819"
     CDS             complement(17612..17671)
                     /gene="ypfM"
                     /locus_tag="ACHW4Z_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR8882386.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4230 a         4759 c         4611 g         4312 t
ORIGIN      
        1 ttaacaatga ttttttcggg ttatatttat ctaaaaaagc agaacattat gtaacttatt
       61 attttctaag cgaaaattta ttgtgcggtc agactaacaa acttgcgtac cacccgaaga
      121 acatgcgaag atttaagtat tctttctttt ttggttttta tttttcattt tctcttttct
      181 tctctttccg gttctgttct ggttttggtt ttttcctttt tatttttatt ctttcttcag
      241 ggttatgcta aaacaccaaa acatttttca ccacaaaagc atccaatcat cttgccagac
      301 caatcacctt aaccggatac actgccggat ataatcccac taacacccag acaccaagca
      361 ccatacctga taactaccgt attcaggaag gaaatgcgag tgatttcacc gtcaccggca
      421 caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca
      481 cttcgtcaat gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag
      541 ctttaccaaa gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga
      601 cgaaatcgac cagcgcgttg atgaccgtca gaaccgcggc gtaacgcaca ggcagcgagg
      661 cgggaagcgc cagcacgtac acgtcagttt tgggatcaag atccagctga agcagcgcct
      721 gttgccaggc actcaccaga tccgtttcat caatcgggat cgccaccggc aaattgcgca
      781 gcggcagttg tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgtgcac
      841 ccgcgccaat caccgtggag cgtacggttt gcgccggaaa ctgcacattc atctcacgca
      901 ggcgcggatg gtcatgcagc gccgttgcca gaagtgggcc aatatcggca aaacagaacg
      961 ggttggcggg ctggtggcga taacattcgc ccacgcctcc agaaagcgta atgatttcgg
     1021 gcgtaacacc tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg
     1081 ttccgtcaat cacttcgaca atcagcgccg ccatccgccg ggtaacctgc accagctgcg
     1141 cgccggtcag cgaacgggcg tcagtgcctg caccgaagca ctcatccaca atcatctgcc
     1201 ccggtttatg agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccaccga
     1261 cgttgagaca ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc
     1321 caccgccgat atcgatattc agtacccgac acagccgttg ttcagaaagg gtttgcgccc
     1381 cggctccgtg accggcgatc acggattcga ggtgcggccc ggcgctggcg acgacaaaat
     1441 cgcccagcga ctgtttttcc ttcatcactt tttccatcag ctgtgcgacc aggctttcgg
     1501 taaactggcc ttccggcagc tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt
     1561 tgtcggctgg cacgctggcg ggcacggacg ccgttaccgg gatcgattca tcacattcgg
     1621 tgatggtaaa acccagtaga tccgccactt cacgggcttc cggggtaata atgctggcgc
     1681 gcagaaccac ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg
     1741 tgatgagttt tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt
     1801 tgctgcacgc tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc
     1861 tcaagcaata actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt tttactgatg
     1921 acggcgattt gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc
     1981 gggtcatttg cgccatggac atagatcagc atgtctacat cctgtagcgt ggtaattaag
     2041 gcgtgatacc agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca
     2101 ttaaattcca cggccagtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat
     2161 aacgttgttt ttcctgcccc gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg
     2221 tattaacttt tggtcatttc acaaagcgta taatttaata agcgacctaa accgctgacg
     2281 gtttgtgata acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccgctg
     2341 aagcggtcga gaaaaccgat atgcacatca gcggctttca gcgctaaatc accggcgatc
     2401 atcgccgttt cgccgggagt tagcgtcata atgccaattg cgcccgcatc cggaacgccg
     2461 attttcttcg ccagttcctc accgggatgc gcgatgagat gcgccagtgt gacctgtttg
     2521 ccgggcacaa attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact
     2581 ttcgtgtatc gtgacaattt ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa
     2641 aaggtgaggt agatcaataa gaatggagag atatttactg ttatgaataa actttcatat
     2701 gcgacgcatg aatataaata aaataatatc gagatatttt atatactgat ttttttatta
     2761 ttcgcaaact cagtaataag ctttgctaat atatttcagg aaaaccgctt accctgaata
     2821 ttcagggtaa gcgtgagagt taagaatgaa ttacagcggt tgggtttgcg cttcaaccac
     2881 ggccagcgcc accatattga cgatacgacg caccgatgcg atcggcgtta acacgtgaac
     2941 cggtttcgcc acgcccatca gcaccggacc gacagtcaca ccttccgagc tggaaacgcg
     3001 cagtaagttg taactaatgc gggcagcttc catgttcggc atcaccagaa tattggcaga
     3061 acctttcaaa gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgctgcatc
     3121 gccgtgcatt tcaccatcaa tcatcagatc tggcgcacga tccctgacca gttccagcgc
     3181 ctgacgcatt ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag
     3241 agcaacgcgc ggctcaatac caaaacgacg gacagtttct gccgccatca aggtgatctc
     3301 cgccagctct tctgcgtctg gttcgtcatt gacgtaggta tcggcaataa aggtgttacc
     3361 actcggcagc agcagcgcat tcattgcacc tgcggtgtga acgccatcgc gataaccaaa
     3421 gacatttttc accacactaa aatgttcatg ataatcgccc accgtaccgc agatcattgc
     3481 atcggcttcc ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc
     3541 acgctgcgcc tgttcctgag tgacaccgcg acgcttcatg atctggaagt attcggtcca
     3601 gtactcttta aagcgcggat cggattcgtt attgacgatt tcaaaatcaa cgcccgcttt
     3661 gatctgcaag cccagtttct gaatgcgcat ttcgatcacg ttcggacgac cgataaggat
     3721 cggtttcgcc agtcccagcg ttaccagttc ctgagtcgca tgcagaacgc gcgcctcttc
     3781 cccttccggc agaactacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat
     3841 aaacaggttg gttttgtaaa cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc
     3901 aatcggacgc gtcgccacgc ccgactccat cgccgctttt gcgaccgcag gagcgatctt
     3961 aacgatcaag cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc
     4021 ctgaacgcca tacgctgaag ccaccacttc actctgttct gcatgggcca gttctgcaat
     4081 cgcgcgtacc gccgccagtt tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc
     4141 gccacggaag atgaacggga agcaaagaac gttgttcacc tggttcgggt agtcggaacg
     4201 gccggtacag atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg
     4261 ttccgggttc gccagcgcca ggatcattgg tgcccgagcc attttcttca ccatttcctg
     4321 ggtcagcact ttcgggccgg aacagcccag gaaaatatcc gcaccttcaa tcacatcatc
     4381 gagggtacgt ttgccgtcat ccaccaccgc atatgcggct ttggtttccg ccatatttgg
     4441 ctcacggccc tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag
     4501 ccccagcgct accagcaggt tcatacaggc gattgctgcg gcacccgcgc cggaaaccac
     4561 catccgcacg tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt
     4621 gctgatgatt gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg
     4681 cagtttctgt tcaatataga aacattctgg cgctttaatg tcttcgaggt tgatgccgcc
     4741 gaaggtcggt tcgagcgcgg cgacgacttc aataaatttg tccgggtcga gttcatcaac
     4801 ttcaatgtca aatacatcaa tcccagcgaa tttcttaaat agaacgccct tgccttccat
     4861 caccggtttg cctgccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga
     4921 gatcaccgca accaggttac cgcgcgcggt atatttgtag gcttttagcg ggtctttttc
     4981 gatttcaaga caaggtgcgg caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt
     5041 tgccagaggc ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc
     5101 aagtgcactt tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa
     5161 ataaagtgtg tgggcaacag cccctgccca caacgtggcg cacattatta ccctgccgga
     5221 gtctacagac tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc
     5281 ctataacact gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt
     5341 tgatgcgttt agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact
     5401 tttcaaggag tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg
     5461 gcagacagct gcgatattga gtccattcgc cattatcatc cccaggatgc caccactaat
     5521 ccttcgctgt tactcaaggc tgccggatta tcacaatatg agcatttaat agacgatgct
     5581 atcgcctggg gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac
     5641 aaactggcgg tcaatttcgg tgctgaaatt ctcaaaatcg tacccggttg cgtgtcaaca
     5701 gaagttgatg cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg
     5761 gtggacttgt atcagcaaca aggcgttgag aaatcacgca ttttgatcaa actggcttcg
     5821 acctgggaag gaattcgcgc ggccgaagag ctggaaaaag aaggtattaa ctgcaacctg
     5881 acgctgctgt tttcttttac ccaggcgcgg gcctgtgcgg aagcaggcgt ttttctgatt
     5941 tcgccgtttg tagggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat
     6001 gtggtggaag aagatccggg cgttaaatcg gtgcgcaata tctacgacta ctataagcaa
     6061 caccactatg aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc
     6121 ttaaccggct gcgatcgact gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa
     6181 gtttctccag tggtacgtaa attaatcccg ccttctcaga cgttcccacg cccagcaccc
     6241 atgagcgaag cggagttccg ttgggagcac aatcaggatg cgatggcggt agaaaaactg
     6301 tctgaaggca ttcgtctgtt cgctgttgat caacgcaaac tggaagatct tcttgccgcc
     6361 aaactataaa ccagccacgg agtcgaaaag accttgccaa tgcgattcgc gcactcagta
     6421 tggatgctgt acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata
     6481 ttgccgaagt gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg
     6541 atcgcgaccg ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctac
     6601 atctgaccgg ttacgacctg ccgctggaag aactgaagaa cttccgtcag ttgcattcga
     6661 aaaccccagg ccacccggag attggctata cgccaggcgt tgaaaccacc accggcccgc
     6721 ttggacaagg tttggcgaac gccgtcgggc tggcgatagc agagcgtaca ctggcggcgc
     6781 agtttaacca gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg
     6841 gctgcctgat ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg ctgggactag
     6901 gcaagctgat tggtttttac gatcacaacg gtatttccat cgacggtgaa acagaaggct
     6961 ggtttaccga cgatacggca aaacgttttg aagcctatca ctggcatgtg atccatgaaa
     7021 tcgacggtca cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag
     7081 ataagccgtc gctgattatc tgccgtacgg tgattggctt tggttcgccg aataaagcag
     7141 gtaaggaaga ggcgcacggc gcaccactgg gggaagaaga agtggcgctg gcacggcaaa
     7201 aactgggctg gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg
     7261 cccgtgaaaa aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa
     7321 aggctcatcc gcaactggca gaagagtttc cccgacggat gagcggtggt ttaccgaagg
     7381 actgggagaa aacgactcag aaatatatca atgagttaca ggcaaatccg gcgaaaatcg
     7441 ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg gccgatgctg cctgagttgc
     7501 tcggcggttc ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtttcgc
     7561 tgaaggaaga tccggcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg
     7621 ctatcgccaa cggcatcgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga
     7681 tgtttgttga atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga
     7741 ttatggttta tacccacgac tcaattggcc tgggcgaaga tggtccgacg caccaggctg
     7801 ttgagcaact ggccagcctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc
     7861 agatggaagc ggcggtgggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac
     7921 tgatcctctc aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga
     7981 ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa gccagatatt attctgattg
     8041 ccaccggttc agagatggaa attaccctgc aagcggcaga gaaattagca ggagaaggtc
     8101 gcaatgtacg cgtagtttcc ctgccctcga ccgatatttt cgacgcccag gatgaggaat
     8161 atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggtattg
     8221 ccgattactg gtacaagtat gttggtctga aaggggcaat tgtcgggatg acgggttacg
     8281 gggaatctgc tccggcggat aagctgttcc cgttctttgg ctttaccgcc gagaatattg
     8341 tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgctg
     8401 tatgttcccc tcaccctaac cctctcccca gaagggcgag gggacggtcc gtgcgctttt
     8461 tagggccgca tctgcgattt tgtttaacgg gtgatccaca acgtgggcct ggcgtctggc
     8521 ccatgccagt tatcgcaggt aggttctgcg gcgtaacgca ccaggcgaaa acgctgaccg
     8581 tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc
     8641 accagttcac gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc
     8701 tggccgttgt tgaacggcaa acgcaaccga accgggcgcg aggctagcgg ctttttacgc
     8761 gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc
     8821 agtgctttat catcggtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag
     8881 cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttacg ttcttcgagt
     8941 gagagtggcg tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt
     9001 acgctgagag gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt
     9061 tgctgagcat caataaacaa caacgccgct tttaagccgc tcagagaaat ggtctgattg
     9121 ccgtcgcgta aggtgatcgc cttcccttcc tgaattatct gcaaaaacgc ggtgatggtt
     9181 gccgtatcat cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaatgcc
     9241 aacggctcac catctaatag cagccgtggc gctatctccc cttcagacgc atccggcgac
     9301 ttcaacccac cgcgctcaat acgtaaaacg gcatcggtat gcgccccagc gctgcggctc
     9361 agggtcatca ccagtccatt atgatcgccc gtattgcgcg ccacacaaaa attttgatta
     9421 ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac
     9481 gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc
     9541 ccagaagttc aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat
     9601 cggatttgtc ggattgagcc aataataaac aacgaaatat tattgctatt tttcagtcca
     9661 ttaaatgtga catttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc
     9721 cggttttgat catctccagc gcctggctga acggcagctc gagcacttca atatcttcat
     9781 cttcaacacc gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt
     9841 ggattagctc agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc
     9901 ccacttcata gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt
     9961 tatccagcag cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg
    10021 tagcgacacg gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga
    10081 tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt
    10141 tgcgcgtgag atcgtaagta atgttgtgca gggtgaaata gttatcggag agaattttgt
    10201 ctttaatgag ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattata
    10261 ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc
    10321 cccagccagt caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc
    10381 agatccgcac cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa
    10441 cggtactggc tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc
    10501 gactccagcc acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct
    10561 ggcatgacaa attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc
    10621 tcgccaagac gcgtgcgcag gaaacgaatt ccgcaaacgt gaccttgctc attcagctca
    10681 agcgccaccg gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcttcacc
    10741 tctttcttcg agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc
    10801 gcgccgtggc gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc
    10861 acgacgttaa gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc
    10921 acctgtttag tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca
    10981 ttgggtaaac ccgctttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat
    11041 tgttccaaaa gcaaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga
    11101 atccccatcg cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa
    11161 gaaggaatgc cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc
    11221 gccacgccat tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata
    11281 atcgccaccc gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc
    11341 aacgcctgat ctgaaatata gcgttcaatg ttgccgatag ttaccgcgcc gtgctcatcg
    11401 cgaatagtac aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc
    11461 ggcagggtgt tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc
    11521 agttcaatcc actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc
    11581 tcgccgaact taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga
    11641 taaatttcat caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc
    11701 gcgggcgttg cctgcatttg ttcgacttta ctcattaccg gcatttcttg tgcagcggta
    11761 ctggcatgcc acggttgatg ctcctgacgt gcggtgcgca agcggcggga tttcgccatg
    11821 ccggagagtg cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcgacacag
    11881 gcaggaccgt tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc ttttactttt
    11941 cctgccgcga cgggtgtcag gacgatttgc atcgtaccaa acggacaggc caccacgcag
    12001 gatttacagc caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt
    12061 gcgccattag ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca
    12121 ctacgttgct gctgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg
    12181 acatgttgct catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga
    12241 cattgctgac tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa
    12301 aaccttattc tttatatgag tgttgttatt acccgactta caggggaacc ggcaatgttc
    12361 atgtgcccag aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca
    12421 ctgactaaat tgcgtttcag cgaactggaa cattaatgat tttttgtgga gaagacgcgt
    12481 gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg
    12541 ctgtcgattc tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct
    12601 gaggctatca atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgacttg
    12661 caaagtggca gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca
    12721 ccggtattaa ccaacctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg
    12781 catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg
    12841 tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag
    12901 cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt
    12961 attttcacgc tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg
    13021 aatcagctgg ttaccgccag tcagcgtatt gaacacgggc agttcgactc gccgccgctg
    13081 gataccagtc tgccgaatga gcttggtctg cttgcaaaaa cctttaacca aatgtcgagc
    13141 gagctgcata aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc
    13201 cacgagacca agcgtcgtct ggaggtgttg tatcagtgca gtgttcccag gcgctgaaca
    13261 ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag
    13321 cagctgaata tctggagctg aacgttgggg ataactggcg gattagcgaa gggaagccaa
    13381 accctgagct gccaatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac
    13441 tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagt gtttcatcga
    13501 tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag ttattgttga
    13561 tggaagaacg tgcgaccatc gcccgcgaat tgcacgactc gctggctcag gtgctttctt
    13621 acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac
    13681 aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc
    13741 tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa
    13801 tgctggatac gttacaaaat caaaccagcg ctaaactgac cctcgactgc cgtctgccaa
    13861 ccctggcact ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc
    13921 tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg
    13981 acggcaatca cacggtttat atccgtgata acgggattgg tatcggtgaa ccgaaagaac
    14041 ccgaaggtca ttatggactg aatatcatgc gcgaacgcgc ggaacggctg ggcgggacgc
    14101 tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctccgcag
    14161 agggtgaggg gaaggggaac acgccggctg aggtgtaaat aattaccctc acaccggccc
    14221 tctcccttta aaggcgagag ggaaaaacgt gcccatattg acctgtaagc gagtgtgtca
    14281 gcaatctcat gataatttac attaactcct ttttttctcc acgattggct cgtaccttgc
    14341 cgctacagtg aagcaagtca agcctacaac aatacgcaga aacacgaggt cctcttttaa
    14401 tggcgaattt ctttattgat cgccccattt ttgcctgggt gctgacaatc ctgttgtgtc
    14461 tgacaggtac cctggcaatt ttttcattgc ccgttgaaca ataccccgat ctcgcgccac
    14521 cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg gaaaacaccg
    14581 tgacccaggt tatcgagcaa aatatgaccg gcctcgataa tctgatgtat atgtcatctc
    14641 agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc accgatccgg
    14701 acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg
    14761 cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga taccaacatt ctgaccattg
    14821 ccttcgtctc taccgatggc tcgatggata aacaggatat tgctgattat gttgccagta
    14881 atattcagga cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc tatggttcgc
    14941 aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag atgacggcta
    15001 aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg caacttggtg
    15061 gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag tcactgctgc
    15121 aaacaccaga acagttccgc gatatcacct tgcgggtgaa tcaggacggc tcagaggtaa
    15181 ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa atacgattat cttagccgct
    15241 tcaatggtaa gccagcctcc gggttggggg taaaactggc ctccagcgca aacgaaatgg
    15301 cgacagcgga gctggtgctc aatcgtctcg acgagctggc gcagtatttc ccgcacggac
    15361 tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt gaagacgtgg
    15421 tgaaaacgct gctggaagct atcgctctgg ttttcctcgt tatgtatctg ttcctgcaaa
    15481 acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg ggaaccttct
    15541 ccgtacttta cgccttcggt tacagcgtca acaccttaac catgttcgcg atggtgctgg
    15601 cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa cgtattatga
    15661 gtgaggaagg actcactcct cgcgaagcca cacgtaaatc gatggggcag atccaggggg
    15721 cactggtcgg gattgcgatg gtgctttcgg cggtatttgt accaatggcc ttcttcggcg
    15781 gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg atggtgctgt
    15841 cagtactggt agcgatgctc ctcactccgg ctctgtgtgc cacgctactt aagccactga
    15901 aaaaaggtga gcatcacggg caaaaaggtt tttttgcctg gtttaaccag atgtttaacc
    15961 gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga
    16021 ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt ttgccgacgt
    16081 cgttcttacc gctggaagac cggggcatgt ttactacctc ggtacaattg cccagcggtt
    16141 caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac ttcacccatg
    16201 aaaaagacaa catcatgtcg gtgtttgcca ccgttggttc tggccctggg ggtaacgggc
    16261 aaaacgtggc gagaatgttt atccgcctga aagactggag cgaacgcgac agtaagaccg
    16321 gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt taaccaaatt aaagaagctc
    16381 gcgttatcgc cagcagccca ccagcaatta gcggtcttgg tagttctgca ggttttgata
    16441 tggagttgca ggaccacgct ggagcgggtc acgatgcgct gatggcagca cgtaatcagt
    16501 tgctggcgct ggcggcggaa aacccggagc taacccgtgt gcgccataac ggcctcgatg
    16561 acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg ggcgttgata
    16621 tcgacgatat taacgacaca ctgcaaaccg cctggggttc gagctatgtg aatgacttta
    16681 tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc
    16741 cagatgacat caatctctgg tatgtccgaa ataaagatgg cggcatggtg cccttctctg
    16801 ctttcgcgac ctcacgctgg gaaacaggct cgccgcgtct ggaacgctat aacggttatt
    16861 ctgcggttga gattgttggg gaagccgcac cgggggtcag taccggtacg gcgatggata
    16921 ttatggaatc gttagtgaag cagctgccaa acggctttgg tctggagtgg acggcgatgt
    16981 cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt tccttgctgg
    17041 tggtattcct gtgtctggcg gcattgtatg aaagctggtc ggtaccgttc tcggtaatgc
    17101 tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac ctggatgcgc gggctggaaa
    17161 acgacgttta cttccaggtg ggcctgttaa cggtcattgg tttatcggcg aaaaacgcca
    17221 tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccatgacctg tttgaagcga
    17281 cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg gcatttatct
    17341 tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag catgcggtgg
    17401 gtactggcgt aatgggcggg atgatttcag ctactattct ggctatttac ttcgtgccgc
    17461 tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa
    17521 aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac ctgctcagcg
    17581 ggttcttgct gtttatactt tgtctcagga attacttacg aagcataact tcgataaagt
    17641 ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt atgggtattc
    17701 tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg actacctccg
    17761 ggggacaaac cttatgtaaa tactatggtc ctgcggtgat aatttgtatg tgatacacag
    17821 caacatttcg agatattcat acggcatcta atactgattt aattctggtt aaaatacaga
    17881 cagataacaa gatgaatatt cttaatgttt ac
//
LOCUS       DAZSNG010000080        17854 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZSNG010000080 DAZSNG010000000
VERSION     DAZSNG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17854
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.081"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(410..3043)
                     /gene="mngB"
                     /locus_tag="ACHW4Z_002820"
     CDS             complement(410..3043)
                     /gene="mngB"
                     /locus_tag="ACHW4Z_002820"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR8882387.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(3061..4977)
                     /gene="mngA"
                     /locus_tag="ACHW4Z_002821"
     CDS             complement(3061..4977)
                     /gene="mngA"
                     /locus_tag="ACHW4Z_002821"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR8882388.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            5147..5869
                     /gene="mngR"
                     /locus_tag="ACHW4Z_002822"
     CDS             5147..5869
                     /gene="mngR"
                     /locus_tag="ACHW4Z_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR8882389.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5972..6841)
                     /gene="sucD"
                     /locus_tag="ACHW4Z_002823"
     CDS             complement(5972..6841)
                     /gene="sucD"
                     /locus_tag="ACHW4Z_002823"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR8882390.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6841..8007)
                     /gene="sucC"
                     /locus_tag="ACHW4Z_002824"
     CDS             complement(6841..8007)
                     /gene="sucC"
                     /locus_tag="ACHW4Z_002824"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR8882391.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(8101..9318)
                     /gene="odhB"
                     /locus_tag="ACHW4Z_002825"
     CDS             complement(8101..9318)
                     /gene="odhB"
                     /locus_tag="ACHW4Z_002825"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR8882392.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(9333..12134)
                     /gene="sucA"
                     /locus_tag="ACHW4Z_002826"
     CDS             complement(9333..12134)
                     /gene="sucA"
                     /locus_tag="ACHW4Z_002826"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR8882393.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12435..13151)
                     /gene="sdhB"
                     /locus_tag="ACHW4Z_002827"
     CDS             complement(12435..13151)
                     /gene="sdhB"
                     /locus_tag="ACHW4Z_002827"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR8882394.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13167..14933)
                     /gene="sdhA"
                     /locus_tag="ACHW4Z_002828"
     CDS             complement(13167..14933)
                     /gene="sdhA"
                     /locus_tag="ACHW4Z_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR8882395.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14933..15280)
                     /gene="sdhD"
                     /locus_tag="ACHW4Z_002829"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14933..15280)
                     /gene="sdhD"
                     /locus_tag="ACHW4Z_002829"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR8882396.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15274..15678)
                     /gene="sdhC"
                     /locus_tag="ACHW4Z_002830"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15274..15678)
                     /gene="sdhC"
                     /locus_tag="ACHW4Z_002830"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8882397.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16059..16166
                     /locus_tag="ACHW4Z_002831"
     CDS             16059..16166
                     /locus_tag="ACHW4Z_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882398.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16372..17655
                     /gene="gltA"
                     /locus_tag="ACHW4Z_002832"
     CDS             16372..17655
                     /gene="gltA"
                     /locus_tag="ACHW4Z_002832"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR8882399.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4259 a         4820 c         4421 g         4354 t
ORIGIN      
        1 cggttggcaa ttaaagataa aaagagacga agcaaaaaca gcacggttga ccttaattct
       61 ttttttggtc actggctaat acgacgcaga gtttcaggat tattaataaa ggcataaatc
      121 tccctcccgc cgatattaac atttaattaa cttataatta tattataatt aactcacccg
      181 tggttattat tttgttaaaa ataaagccac atatacgtag cttgagagtg aagataaaca
      241 caaaattacc tgatgcgcta cgcttgccag tgtctacatc atctctgcaa tatattgaat
      301 ttgcctgctt ttgtagaccg aataaagcgt tcatgccgta tccggcatga acaaagcgcc
      361 ctttgtcaac aatctggccg cgcatatgtg gcttcggttt gattgctatt caggcaagcc
      421 ggtaactgaa cgtccgtgac tggccgggta aaaaaggccc cgatagattt gaaccttgat
      481 tttcctcggt ggtaatgtgt tcatccatca tcgtttctga gcaagaaatc acctcgcgac
      541 tgaaagcaac agtcgcatca caggttgctg attcagccgg attaaacagc cgtaaaatca
      601 cttcttgtcg gtcttcagct ttcttaagtg cgcttatcag gcatcccact gggggcattt
      661 tcaacaaact ataactttcc ggcacgttga atccggcttt gttgagcttc atcgcatccc
      721 atgggatttt gttgtaacac tgtactggag tcagccatgc tcgcgcctgc tgagctacac
      781 cagcggcggt tggcgtaccg gtataactca ataaacttag gcgacaagaa agcagaccac
      841 gtagttgtga gtccgggact ggcattttaa ttcccgaagg ccgcctaggc cttaaaagca
      901 gatcttcttt ccccagtaag cccacgccgc gcagcaacgt aatggcaaag gttttcttct
      961 cttcaccgat gacttcaaat tcacgtagcc cttcgctaaa gacagccatg ccgttacgcc
     1021 cttcctgtaa ggctgcatag ttgagcatat tccataccgg aaccggcgct tctttccagc
     1081 tttcttgctg ccagttgttc attgcactgt cgttcacggg gcgcgttagc gaaccaaact
     1141 gggtatctgc cagaacacta tcggtgttaa aagatgtagg gatcaggacg cgaaggcgat
     1201 gatcgtcagc ctggttatca aggttgatat ccacatcaat gcgcctgttg ttatgactaa
     1261 gagtgacaac caactcgaca tctactctgc cagtggattg tcttgcgctg cgttgttgca
     1321 gattacgcgg caccgccatt tcatagcgga taacagccct gctctgccag gcctcatgaa
     1381 taatctcgca ttgcggtttc gcattcgccg aagtaattac ccactcttct tttgcgggtg
     1441 aatagtcata ttcatcacca tcatcagagc tttcttcaat ttgcaacacc cggtcatagc
     1501 gcacaccgct atctttatct accagttgta gcgttccatc ctcattgagc gcaatttgcc
     1561 agaaagcatt ttccagtatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct
     1621 gattcgcttc gatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgta
     1681 tatcaaactc cataaaggga tcgtaattac cgtaatgaac tatttgccga tcgattaggc
     1741 ctggatcgat ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctc
     1801 gtagattaaa ctggctggcg cgcagccgca cagtggtgtt gatcacttct tcccgcggcc
     1861 agggcatcag gttaaacagg acgagtttgt cggcgtcgct ctgcggcatg ttgtcggcga
     1921 ttttacgcat atagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg
     1981 cgacgatttc gcgatgaact ttgtcacgac agcagcagcc gatgctgtcg tgggcatgat
     2041 tttttaagat ctctttccac attttctcca gcaagccgtg gtggtatcca aaacccaacg
     2101 tcctggccag cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt
     2161 gggcaacttt gatatccatg cgcgtggaac cgatggtgcg atgcacgcgc atatatttgc
     2221 catcaataaa ttcccctttc agggttgcca gattatctcg ctgcgcttcg atcttctcaa
     2281 atacctcttc aaagcggctc atcacaaact ttcgttgagg ataaatttcg cgcagtttct
     2341 ccatcacttc gaagatattt tgctgcaatg gcatctgatc atgcccattc ggcagcaaaa
     2401 tctctttggt tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttgcgta
     2461 atccgttttc gtccgcaggt aagtacttac cgatggcata acccaacggc atcacctgcg
     2521 ccgtcacttc gctaccgtca ctgctttgcc acaaaaactc ggttttatcc gtaccgtggc
     2581 gctcagaaca tccacgccag aacatggtgc aggtaatgcc aaatccattg tagatatgcg
     2641 gcagttgccc ggacatgcca aaggaatcgg gtaaataacc tattttcatc ggctcgccaa
     2701 acgcgaggca gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa
     2761 tcgtggtatc ggtctgggta taccaggggc cgataatcaa cttgccggct tctaccagtt
     2821 tcttcaagcg gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt
     2881 gcccgtcgag tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt
     2941 ccatattatt gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc
     3001 agtgcatatg cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt
     3061 ttatggcatt acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat
     3121 caggagaatt gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc
     3181 cgccataaca ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccagcacc
     3241 gggtgtcgaa aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag
     3301 cacaaacgaa ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt
     3361 aatacctgcc agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa
     3421 caggcgcggt gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga
     3481 ggcgaaaatg gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata
     3541 agcggcttta ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc
     3601 cagcaacagc gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat
     3661 ccaggcgaca ggttccccca ccacaaacag catcagactg cccgctccca atgtaccgag
     3721 caccgggtag agataaaaag tcaggaagcc attgaattta ctgctaagac gcaagtgatt
     3781 tttcatccag cgtatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc
     3841 ggagccgatc atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc
     3901 tgccagcgaa taggcggtat aggctgccag caccggtacc atcaaaattc cgagcagccc
     3961 gccgcccagt ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag
     4021 cccgaagatt tgcgacagta ataccgcgac cgccagcacc gtcccccccg cgacaatcaa
     4081 cggtacggca aaagagatcc cgctcaacag tgcctgtttc agctccgttt tgacgctttt
     4141 caccggttgc gtatcttgct gtaaggtacg cgtctcatcg ccacgctcga gggtaagcga
     4201 ttgttggatc aacgcttctg catggcgaat cggctcggca acaggcacag aaagcgcagg
     4261 aatgccgtta aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcagt
     4321 tgcactattg agttgatccg ccgttaaacg cccttcaata ccgttagcac cttgcttttc
     4381 aacgtagaca ttcacgccga gtttgcgtcc ggctttttcc agatactccg cagccatgta
     4441 ggtgtgagca atacccgccg ggcaggccgt tacgcaaacg atagttggcg cgttggaaaa
     4501 agaggcagaa ggttgcgtgc ctcccttgtc atccagcgcc gagagcaatt catcaggcgt
     4561 cgtcgccgac tgtatacgcg cccgaatctc atcatccgcc aggcgcgtgg tcagcgctgt
     4621 cagcagttgc atgtgcgtag tacccgcttc attgagggga atcgccagca acaccactaa
     4681 atcaaccgct tccgggccat ccacgccttc ccactgaagc ggctcgctga gtgtggcgac
     4741 cgcaaacgcc gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcact
     4801 taaggcggtc gggccaaggc tttcacggcg atacacttct ttcagaaatt gctcagtact
     4861 ggaaattttc cccagagcag caagacgttg agtcaacgcg tgaatggcct cttcacggct
     4921 ggtaaagcgc gcattcagac acaacgcatc gcggtgggtc agagtcgtca ggttcatcat
     4981 gatcctcact tgatcgtttt tatagtcgcg ccagtgtgcc cgacaaaaca ataccattgc
     5041 gtaatcaatt tcacttgatt gaaattattt gcattttatt tgtattgttt acaggatgag
     5101 tttatcgggc ataatggcta aattcgtatt aatgagatgc taaataatgg gacacaaacc
     5161 cttataccga cagattgctg atcgcattcg tgagcaaatt gcgcgtggcg agcttaaacc
     5221 tggcgatgcg ttacctaccg aatcggcatt acaaacagag tttggcgtca gccgggttac
     5281 ggtgcgtcag gctttgcgcc agttagtcga gcaacagatc ctcgaaagca ttcagggcag
     5341 cgggacttac gtcaaagaag agcgggtcaa ttacgatatt tttcagttaa ccagttttga
     5401 tgaaaaactg tcggaccgtc acgtcgatac acacagtgaa gttctgatat ttgaagtgat
     5461 tccggctgac gattttcttc agcaacagct acaaatcacc gtgcaggatc gtgtttggca
     5521 tgtgaagcgc gtgcgctatc gcaagcaaaa gccaatggcg ctggaagaaa cctggatgcc
     5581 gcttgcgttg ttcccggatc tcacctggca ggtcatggaa aattcgaaat atcactttat
     5641 tgaagaagtg aagaagatgg ttatcgatcg tagcgaacag gaaatcattc ctctgatgcc
     5701 aaccgaagag atgagccgtc tgcttaatat cagccagaca aagccgattc ttgaaaaagt
     5761 atcgcgtgga tatttagttg atggtcgggt gtttgaatat agccgcaacg cttttaatac
     5821 cgatgactat aaatttaccc tgatagcgca gcgaaaatca tcgcgataag cacaaaaagg
     5881 ccatcatacg atggccaacc atgtcgaaac cgacagccct tcgccattcc tgtagaggga
     5941 agcggcgagg gctatttctt attacagata tttatttcag aacagttttc agtgcttcac
     6001 cgatatccgc caggctgcga acggttttca cgcctgcggc ttccagagca gcgaatttct
     6061 catccgcagt ccctttccca ccggcaatga tcgcacccgc gtggcccata cgtttgcctt
     6121 tcggcgcagt cacaccagcg atgtaaccca caactggctt ggtaacatgc tctttgatgt
     6181 acgctgccgc ttcttcttca gcgctaccgc cgatctcacc gatcatcacg atcgcttcgg
     6241 tctgcggatc tttttcgaac atttcgagaa tgtcgataaa gttagagcca gggatcgggt
     6301 caccgccgat accgacacag gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg
     6361 cttcataggt cagtgtaccg gaacgggaaa cgatacccac tttacccggt ttgtgaatgt
     6421 gacccggctg gataccaatt ttgcattcac ccggggtgat aacgcctggg cagttcgggc
     6481 cgatcatacg aacgcctgct tcgtccagtt tcactttcac ggtcagcata tccagcgtcg
     6541 ggatgccttc agtgatggtg ataatcagtt tgatgcctgc atcgatagct tccagaatgg
     6601 agtctttgca gaacggtgct ggtacgtaaa taacagacgc ggaagcgcca gtagcagcaa
     6661 cagcttcacg cacggtgttg aacaccggca ggccgaggtg ggtagtgccg cctttacctg
     6721 gagttacgcc accaaccatt ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc
     6781 cctggctacc ggtaaagccc tggcagataa ccttggtgtt tttatcaatt aaaatggaca
     6841 ttatttcccc tccactgcgg caacaacctg ctgagctgca tccgtcagac cttttgctgc
     6901 aataatattc aggccgctgt cagccagttt cttcgcgccg agttcggcgt tgttaccttc
     6961 cagacgtacc acgaccggta cgttaacacc cacttccgct accgcgccga tgataccgtc
     7021 agcgatcagg tcgcaacgaa cgataccgcc gaagatgtta accagaacgg ctttcacttt
     7081 gtcgtcagag aggatgattt tgaacgcttc ggttacacgt tctttggttg cgccgccgcc
     7141 aacgtccagg aagttagccg gttcgccgcc gtgcagttta actatgtcca tcgtacccat
     7201 cgccagacct gcgccgttaa ccatacaacc gatgttaccg tccagcgcaa cgtagttcag
     7261 ttcccactgt gcagcctgtg cttcacgcgg atcttcctgc gactggtcac gcatttcgcg
     7321 cagatcaggc tggcggaaca gggcgttgcc atcagcgccc agtttgccgt cgaggcaaat
     7381 cagatcgccc tgtttggtga tgaccagcgg gttgatttcg atcagcgcca ggtcgcgctc
     7441 caggaaaatg gtcgccaggc ccatgaagat tttggtgaac tgctgaacca gtttaccttc
     7501 cagacccagt ttgaacgcca gctcgcgtcc ctgatacggc atcgggccag tcagcggatc
     7561 aagcgcaact ttatggatca ggtgcggagt ttcttccgcc actttttcga tttccacgcc
     7621 gccttcggtg gaggccataa agaccacgcg acgggaacta cggtctacaa cggcaccgag
     7681 atacagctct ttagcgatat cggtcgccgc ttcaaccaga atctggttaa ccggttggcc
     7741 attggcatct gtttgatacg ttaccagacg cttgcccagc cagttttctg caaaagcacg
     7801 gatgtcttct ttgctgttta caactttcac accgcccgct ttaccgcggc caccagcgtg
     7861 aacctgacat ttcactaccc acggaccggc accgattttt gaagcggctt cttctgcttc
     7921 gcgcggagta gtacaggcat aacccaccgg tgctggtaag ccatagcggg caaacagttg
     7981 ttttgcctga tattcatgta agttcatgtg ttctgtccat ccttcagtaa tcgttatctt
     8041 ttaaaccgta gaccgacacc ctatccacgt cggtctacag tgcaggtgaa gcttaaacta
     8101 ctacacgtcc agcagcagac gcgtcggctc ttccagtaac tctttgatcg ttaccaggaa
     8161 gcccacggat tcgcgaccat cgatcagacg gtgatcgtag gacagcgcca ggtacatcat
     8221 cggcaggatc tcaacctgac cattcaccgc catcggacga tctttgatag cgtgcatacc
     8281 cagaattgcg ctctgcggcg ggttgatgat cggcgtagac atcagggaac cgaacacacc
     8341 accgttggtg atggtgaagt taccaccggt cagatcttca accgtcagct tgccatcacg
     8401 gcctttgact gccagctctt tgattttctt ctcgatgtct gccatgccga gggtatcgac
     8461 atcacgcaga accggcgtca ccaggccgcg cggcgtagaa accgccatgc tgacgtcgaa
     8521 atagttgtgg taaaccacgt catcgccgtc gatagacgcg ttcacttccg ggtaacgttt
     8581 cagggcttca accaccgctt tcacgtagaa ggacataaag cccagacgga tgccgtggcg
     8641 tttttcaaac gcttcaccgt actgcttacg cagatccata atcggcttca tgttgacttc
     8701 gttgaacgtg gtcagcatgg cggtggagtt tttcgcttcc agcagacgct ctgccacacg
     8761 cttacgcagg cgagtcatcg ggacacgttt ttcactacgt gcagccagag ccggttgcgc
     8821 cgccggagca gccgctgccg gagcagactc tttcgccggg gctttcgcca gatgtttttc
     8881 cacatcttca cgagtcagac gaccacccac accggtgcct ttaatggcgc tggcgtcgag
     8941 attgtgttca gccagcaggc gacggatcgc cgggcttaac gcatcgttgt tttgctcttc
     9001 cagagacgcc tgctggcgtt gcgccggagt ggacgctttc tcttcagatt tggcgctggt
     9061 ttctttaccg gcgctgttgc cttcacgcag gcgaccaagg atctgacgag acgttaccgt
     9121 tgtaccttca tcttccagaa ccgcatccag aatgccgtct gctgatgccg gtacttccag
     9181 taccactttg tcagtttcga tttctaccag cacttcatca cgtacgactg cgtcgccggg
     9241 ttttttatgc caggttgcga cggtggcatc ggctacggat tcaggcaggt cagggaccag
     9301 aatatctacg ctactcattg tgtatccttt atttattcga cgttcagcgc gtcattaacc
     9361 agatcttgtt gctgtttctg gtgaacggac atatacccta ccgccggaga ggcggaggcc
     9421 gggcggcctg cataacgcag agaagcccca aacggaatca cttcacggaa atgatgctgg
     9481 ctgcagtacc atgcgccctg gttgagcggc tcttcctggc accagacaaa atccttgacg
     9541 tgagcaaact gctgcaacac ttcctgcatc gctttatgcg ggaacgggta gagttgctcg
     9601 atacgcacaa tggcgacatc gtgttgattg ttcttacgac gctgttccag caggtcgtaa
     9661 taaaccttac cagaacacat cactacgcgc ttcacgccct tcggatcaag ctcgtcgatt
     9721 tcaccgatgg ctggcaggaa ggtgccgttc gccagttctt cgaggctgga aaccgccagc
     9781 ggatgacgca gcagggattt cggcgacatc acgaccagcg gacgacgcat cccgcgcagc
     9841 gcctgacgac gcagcatgtg gtaaacctgt gccggggtag acggtacgca aacctgcatg
     9901 ttttgctcag cacaaagttg cagataacgt tccagacgcg cggaggagtg ctccggcccc
     9961 tgcccttcgt aaccgtgcgg cagcaacatc accagaccac acatccggcc ccatttctgc
    10021 tcgccagagg agatgaactg gtcgataacc acctgcgcac cgttggcgaa gtcaccgaac
    10081 tgcgcttccc agatggtcag ggtgcgtggt tctgcggtgg cataaccata ttcaaacgcc
    10141 agcacggctt cttctgacag tacggagtcc cagacacgga acgcgccctg accgttatgg
    10201 atgtgttgca gcggcgtgta agtggaaccg ttagactggt tgtggatcac cgcgtggcgg
    10261 tggaagaagg taccgcgacc ggagtcttca cccgacaggc gaaccggaat gccttcatca
    10321 accagcgtgg cgtaagcgag gttttccgca ccgccccagt cgaacagttt ctcaccggct
    10381 gccatcgcct ggcgatcgcc ataaatcttg gcaacgcgag actgcatttc aactgcttct
    10441 ggtaccgtac tgatacgttt agccagttcc tgcaggcgct tcatctcaac tttgttcggg
    10501 tactcttcgt cccattcgtg gttgaggtac ggcgaccagg tgaaagagtg catgttcatc
    10561 ggacgccact ctgccactac gcaatcgcca gcatccagcg catcgcggta caggttaacc
    10621 atctcggtgg catcttccag cgtcgccact ttttcctgct ccagcttgtc agcgtagatt
    10681 ttgcgcggtg tcggatgttt tttgattttc tgatacatca gtggctgggt tgcgctcggc
    10741 tcgtcggctt cgttgtggcc gtgacggcgg tagcatacca ggtcgatgaa gacatcacgt
    10801 ttaaaggtgt tacggaaatc gagcgccaga cgggtcacaa aggcaacggc ttccggatca
    10861 tccgcgttaa cgtggaaaat cggtgcctga accatcttac cgatatcagt acagtacggt
    10921 gtagaacggg catccagcgg gttagaggtg gtgaaaccaa cctggttgtt gataacgata
    10981 cgtaccgtac cgccaacttc ataaccacgc gctttcgaca tgttcagagt ttcctgaacc
    11041 acgccctgcc cggtcactgc ggcgtcaccg tggatggtga ttggcagcac tttgttgctg
    11101 ttcggctcat caagtctgtc cagacgggca cgaacagagc cgataacgac cgggcttaca
    11161 atctcaaggt gagacgggtt aaacgccagc gccaggtgca ccaggccgcc atcggtctgg
    11221 aagtcagacg agaagcccat gtggtatttc acgtcgcctg tgccgaggtg ttctttatgt
    11281 ttaccggcga actcgtcgaa caagtcttgc ggttttttac ccagcacgtt caccagcacg
    11341 ttcagacgac cacggtgcgc catcccgaga accacttcgc gggtgccgct gttgccagcg
    11401 tggcggatca tctctttaag catcgggatt aacgcgtcac cgccttccag cgagaagcgt
    11461 tttgcgccag ggaattttgc gccgaggtaa cgctcaaggc cttcagcggc ggtcagttcg
    11521 cttaagaagc gttttttctc ttcgctattg aaagtcgcgc gaccagactc gatacgctgt
    11581 tggatccagc gtttttcttc ggtgctggtg atgtgcatat actcggcacc aatcgggccg
    11641 cagtaggttt gcttgagggc ggaaataagc tcgccgagtt tcatggtttc tttgccgctg
    11701 gcaaatgaac cgacgttgaa ggtttcctgg aagtctgctt cggtcagatc gtggaaagac
    11761 ggatccagat cggccacttt atcttgctgc cacagtccca gcggatctag attcgcatgc
    11821 tggtgaccac ggaagcggta tgcgttaatg agctgcagga ctttaacctg cttcacattg
    11881 gtgtcagggt cggagatcgt tgaagagtaa cgtgaagcgt ctttcgccag gcggcggaaa
    11941 tattcacgcg tttgagagtg gaattgatcc ggtttgactc ccgtaccagg taactgctgg
    12001 aacgtcgaac gccagttagc gtcaaccgag tcaggatcgg ttaagaagtc ttcatagagc
    12061 tgttctatcc agctctggtt tgcgccagag aggtaagaag agtccaacca ggctttcaaa
    12121 gcgctgttct gcatcgtgat cccttaagca tcttttttat gcttacttcg ccgtggatac
    12181 taccacgcac agtgatgtgc gtgccggggt tcacttgcga gctcttgtct acgtagtggc
    12241 tcgcgaagga acctttaaaa actgtcttag tgtcaggggc agtttttaaa gatttcctgc
    12301 atttctttgt tacgcctgat gcgctgcgct tatcaggcct gcgggtaatg acttgtaggc
    12361 cggataaggc gttcacgccg catccggcac tggttgcctg atgcaacgct tgcgcgtctt
    12421 atcaggccta cagtttacgc attacgttgc aacaacatcg acttgatatg gccgatggcg
    12481 cgcgtcgggt tcagcccctt cggacataca ctgacgcagt tcatgatgct gtgacagcgg
    12541 aatacgctga atgcatcgct caaaccgtcg aggcggctgt cagtctcggt atcacggcta
    12601 tcgatcagga aacgatatgc cgctaacaag cctgccgggc cgataaactt atcgggattc
    12661 caccagaaag acggacaaga ggttgaacaa catgcgcaga gaatacattc atacagcccg
    12721 tcgagttttt cgcgctgctc tggcatctgt aaatgctcgc gagctggcgg attttgtcca
    12781 ttattcaaca ggtaaggctt aattttctca tattgcgcat agaattgtcc catgtctacc
    12841 accaaatcgc ggatcaccgg taaacctggc agcgggcgaa tcacaatctt cttgcccggc
    12901 tggttgagtg ccgaaatcgg ggtaatacag gccagaccat tcttgccgtt catgttcaga
    12961 ccgtcggaac cgcacacacc ttcacggcag gagcggcgga acgacaggct gggatctttc
    13021 tctttcagct ggataagcgc atccagcagc atcatgtcgc gaccttcttc cgcttccagg
    13081 gtgtaatcct gcatacgcgg agcatcatca acatccgggt tatagcgata aattgaaaac
    13141 tcgagtctca ttttcctgtc tccgcattag taagtacgaa tcttcggcgg gaatgccggg
    13201 cgcagtttcg gttccatgtt gacgcttcgg cgcgtcatgg attccgactc aggcagatac
    13261 agggagtggc acagccagtt ttcatcatca cgatccggga agtcgaagcg gctatgtgcg
    13321 ccacggcttt cggtacggaa gttggcagaa acagccgttg catacgccgt ttccatcagg
    13381 ttatccagtt ccaggcactc aacgcgctgg gtgttgaact cgctggaagt gtcatccaga
    13441 cgggcatttt tcagacgctc gcggatcact ttcaactgct caagcccttt cgccatcgca
    13501 tcaccttcac ggaagaccga gaagttatgc tgcatacatt cttgcagcgc tttacggatc
    13561 gccaccggat cttcaccgtt acgattattg ttccaccggt tcaggcgatc cagagacgct
    13621 tcaacatcag actcgctggc atcgtgcagt gcgccctgct cggcgataga ctcttgcaga
    13681 tgcagacctg ccgcgcgacc aaagaccacc aggtccagca gcgagttgcc gcccagacgg
    13741 ttagcgccgt gtaccgatac acaagcgatt tcaccaacgg caaacagtcc cggaacaacc
    13801 acatcttcgc ctttctcatt cacagtcagc gcctgaccgg taactttggt cggaataccg
    13861 cccatcatgt agtgacaggt tgggataacc ggaatcggct ctttcaccgg atcgacgtga
    13921 gcgaaggtac gggaaagctc caggataccc ggcagacggg attcgagaac ttctttaccc
    13981 aggtgatcga gtttcagttt cgcgtgtggc ccccacggac catcacagcc gcgaccttca
    14041 cggatttcaa tcatgatgga acgcgccacc acatcacggc ccgccaggtc tttggcgttc
    14101 ggcgcataac gctccataaa gcgttcgcca tgtttgttca gcagataacc gccttcacca
    14161 cggcaacctt cggtgaccag tacgcccgca ccggcaatgc cggtcgggtg gaattgccac
    14221 atttccatat cctgcaccgg tacgcctgcg cggatagcca tgccgacacc gtcgccagtg
    14281 ttaatgtggg cgttagtggt ggactgataa atacgccctg ctccgccagt cgccagcacg
    14341 gtagcgcggg ctttgaaata aaccacttca ccggtttcga tgcacagtgc ggtacaaccc
    14401 accaccgcgc catcctggtt tttcaccaga tccagcgcat accactcgga gaaaatggtg
    14461 gtgtggtttt tcaggttctg ctgataaagc gtgtgcaaca gtgcgtgacc ggtacggtca
    14521 gcagccgccg cagtgcgtgc cgcctgctcg ccgccgaagt ttttcgactg accgccaaac
    14581 ggacgttgat agatacgacc atcatcgaga cgcgagaacg gcaggcccat gtgttcgagt
    14641 tccagaatcg cttccggccc ggttttacac atatattcaa tcgcgtcctg gtcaccgata
    14701 tagtccgacc cttttacggt gtcgtacata tgccattccc agttatcttc atgggtatta
    14761 cccagcgcaa cggtaatgcc gccttgcgca gaaacggtat gggaacgggt cgggaagact
    14821 ttagagagca gggcacaggt ctggccgctc tgggaaattt gcagcgccgc gcgcatacct
    14881 gcgccaccgg caccaatcac aactgcatca aattctctga ctggcaattt catcacacac
    14941 cccacaccac aacgaatcca taaatcacgt aaaccaccag tgcaacgaca atcaccagtt
    15001 gcagcatcag gcgcaaggcc agcggtttaa cgtagtcggt caacacctgc cacatgccga
    15061 tccaggcatg aatcaagata gaaaacagcg ccagcagggt gaacactttg gtgaacgcag
    15121 aggcgaagaa accgatccag acttcatatg tcagttcgcc actggtggcg aaaaaaccga
    15181 ccatataaat gatgtagagc gtcaggacga tagcggtagc gcgaacgagg atgaaatcat
    15241 gtacgccatt gcgtcctaat gcggaggcgt tgcttaccat acgaggactc ctgcgagaag
    15301 tgaaagcacg acagtaataa caaaggagat tttggcggag cgtttacccg cttcgaatgt
    15361 ttcttccaga tagccaaaat ccatcatcat gtggcgaata cctacgacga cgtgatacgc
    15421 cagagcggta aggatgcccc acatgataaa tttgacgaag aagctgccca taatcgcgga
    15481 agcttgctcg aaaccttcag gggaagagag gctggtaccc agaagccact gcaggatgcc
    15541 cactgcaaca aaggtgatca caccggaaac gcgatggaga atggacgcta tcgccgtgat
    15601 ggggaaccgg atggtctgta ggtccagatt aacaggtctt tgttttttca catttcttat
    15661 catgaataac gcccacatgc tgttcttatt attccctggg gactacgggc acagaggtta
    15721 actttctgtt acctggagac gtcgggattt ccttcctccg gtctgcttgc gggtcagaca
    15781 gcgtcctttc cataactgcg cgtcatgcaa aacactgctt ccagatgcga aaacgacacg
    15841 ttacaacgct gggtggctcg ggattgcagg gtgttccgga gacctggcgg cagtataggc
    15901 tgttcacaaa atcattacaa ttaacctaca tatagtttgt cgggttttat cctgaacagt
    15961 gatccaggtc acgataacaa catttattta atttttaatc atctaatttg acaatcattc
    16021 aacaaagttg ttacaaacat taccaggaaa agcatataat gcgtaaaagt tatgaagtcg
    16081 gtatttcacc taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga
    16141 cagttattag tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt
    16201 attcgtcatc aatggattct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc
    16261 ctctttcaca gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg
    16321 caaatttaag ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat
    16381 acaaaagcaa aactcaacct caacggggac acagctgttg aactggatgt gctgaaaggc
    16441 acgctgggtc aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt
    16501 gacccaggct tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat
    16561 gaaggtattt tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac
    16621 ctagaagttt gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa
    16681 tttaaaacta cggtgacccg tcataccatg atccacgagc agattacccg tctgttccac
    16741 gctttccgtc gcgactcaca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg
    16801 gcgttctatc acgactcgct ggatgttaac aatcctcgtc atcgtgaaat tgccgcgttc
    16861 cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag
    16921 ccatttgttt atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc
    16981 tccacgccgt gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt
    17041 ctgatcctgc acgctgacca tgaacagaac gcctctacct ccaccgtgcg taccgctggc
    17101 tcttcggggg cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct
    17161 gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa
    17221 cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc
    17281 ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc
    17341 catgaagttc tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg
    17401 gaaaacatcg cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat
    17461 ttctactctg gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatt
    17521 ttcgcaatgg cacgtaccgt tggctggatc gcccactgga gcgaaatgca cagttacggt
    17581 atgaagattg cccgtccgcg tcagctgtat acaggatatg aaaaacgcga ctttaaaagc
    17641 gatatcaagc gttaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcaaatg
    17701 gcgggttaat ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc cactatcgga
    17761 cattcatata ttctaaaaaa aaatacatcg gtataaatta taaacaaata gatgattttt
    17821 aatcatctat cttcctctca gttacatgaa taaa
//
LOCUS       DAZSNG010000081        17780 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZSNG010000081 DAZSNG010000000
VERSION     DAZSNG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.082"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            176..859
                     /locus_tag="ACHW4Z_002833"
     CDS             176..859
                     /locus_tag="ACHW4Z_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8882400.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            903..5565
                     /locus_tag="ACHW4Z_002834"
                     /pseudo
     CDS             903..5565
                     /locus_tag="ACHW4Z_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5933..7588
                     /gene="lldP"
                     /locus_tag="ACHW4Z_002835"
     CDS             5933..7588
                     /gene="lldP"
                     /locus_tag="ACHW4Z_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR8882401.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7588..8364
                     /gene="lldR"
                     /locus_tag="ACHW4Z_002836"
     CDS             7588..8364
                     /gene="lldR"
                     /locus_tag="ACHW4Z_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR8882402.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8361..9551
                     /gene="lldD"
                     /locus_tag="ACHW4Z_002837"
     CDS             8361..9551
                     /gene="lldD"
                     /locus_tag="ACHW4Z_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR8882403.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9747..10220
                     /gene="trmL"
                     /locus_tag="ACHW4Z_002838"
     CDS             9747..10220
                     /gene="trmL"
                     /locus_tag="ACHW4Z_002838"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR8882404.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10273..11094)
                     /gene="cysE"
                     /locus_tag="ACHW4Z_002839"
     CDS             complement(10273..11094)
                     /gene="cysE"
                     /locus_tag="ACHW4Z_002839"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR8882405.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11174..12193)
                     /gene="gpsA"
                     /locus_tag="ACHW4Z_002840"
     CDS             complement(11174..12193)
                     /gene="gpsA"
                     /locus_tag="ACHW4Z_002840"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR8882406.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12193..12660)
                     /gene="secB"
                     /locus_tag="ACHW4Z_002841"
     CDS             complement(12193..12660)
                     /gene="secB"
                     /locus_tag="ACHW4Z_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR8882407.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12723..12974)
                     /gene="grxC"
                     /locus_tag="ACHW4Z_002842"
     CDS             complement(12723..12974)
                     /gene="grxC"
                     /locus_tag="ACHW4Z_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR8882408.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13116..13547)
                     /locus_tag="ACHW4Z_002843"
     CDS             complement(13116..13547)
                     /locus_tag="ACHW4Z_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR8882409.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13792..15336
                     /gene="gpmM"
                     /locus_tag="ACHW4Z_002844"
                     /gene_synonym="pgmI"
     CDS             13792..15336
                     /gene="gpmM"
                     /locus_tag="ACHW4Z_002844"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR8882410.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15370..16629
                     /gene="envC"
                     /locus_tag="ACHW4Z_002845"
     CDS             15370..16629
                     /gene="envC"
                     /locus_tag="ACHW4Z_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR8882411.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16633..17592
                     /locus_tag="ACHW4Z_002846"
     CDS             16633..17592
                     /locus_tag="ACHW4Z_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR8882412.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17579..17780)
                     /locus_tag="ACHW4Z_002847"
                     /pseudo
     CDS             complement(17579..17780)
                     /locus_tag="ACHW4Z_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4452 a         4620 c         4648 g         4060 t
ORIGIN      
        1 catacaaaca cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac
       61 aatatttaat cgacacacat cactgtcatt aatgtaaagc aacatagctc accaataata
      121 caatggacaa ctctaccggc attgagcgct ggtcggagga aataaggatt tttacatgaa
      181 cttgaagaag atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg
      241 tgactacatt gaagaaggca agccagaaag tagtttactg aaacaacaag aagaacataa
      301 taataaaatt gtgctacttg aaaaacaaca agcacagcta aagagtcaac tcgaaactat
      361 tcaaaaacaa caaactggca taataaacag tacaaagact ttaactcacg taattaaatc
      421 cgttaaagac caacaaaata cttttatttt taccgagttt aacccagcaa aaacgaagta
      481 tttcatcctg aacaacggtt cggtggcttt agcgggtcga gtgttatcca ttgacgccac
      541 agagaatggt agtgttattc atatctcact ggtcaactta ttaagtacac ctatctcaaa
      601 cattggtttt aatgcgacat ggggtggtga aaaacctgtc gatgccaaag agtttgcccg
      661 gtggcaacaa ttacttttca acacatcaat gaaatccaca ttgaaattat taccaggtca
      721 atggcaagac attaatttga ccctgaaggg tgtatcgccc aataacctgg gatatctgaa
      781 gttagccatc aacatggaaa atattcagtt cgacaatctt ccctctgctg aaaatcggca
      841 gaaaagaagc aaaaaataaa tgcagttagt acgcttataa caaggaatag cgagcatcat
      901 taatgaacaa aatatttaaa gttatctgga accctgcgac agggaattat actgttacca
      961 gcgaaacggc aaaaagccgt ggcaagaaat ctgggcgcag taagctgtta atttctgcgc
     1021 tggttgcggg tggaatgttg tcgtcgtttg gggcattggc gaatgccggg aatgacaacg
     1081 gtcagggtgt tgattacggt agtggatcag ctggcgacgg ctgggttgct ataggcaaag
     1141 gggcgaaagc aaatactttt atgaacacca gtggttccag tactgctgtg ggttatgacg
     1201 ctatagctga aggccaatat agctctgcca tcgggtcaaa aacccatgcg attggtggtg
     1261 catcaatggc ctttggggtt agtgcaatat cagaaggcga tagaagtata gcactgggtg
     1321 cctcttcgta ttcattgggc caatactcaa tggccctcgg ccgttattca aaagcattgg
     1381 gtaaattgtc tattgctatg ggggactctt ccaaagcgga aggagcaaac gccattgccc
     1441 tggggcgtag cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg
     1501 ccagcgcagc gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg
     1561 ctattggcaa taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc
     1621 ttgccgataa agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg
     1681 caatcgccat aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg
     1741 gtagccagtc gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg
     1801 ataagagtct tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg
     1861 gcgcagattc cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac
     1921 gcaagatcgt taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg
     1981 gttcacagct ttatgccatt agcgactcgg tagcaaaaaa ggcttggagg aggggctgca
     2041 gtagatgttg atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa
     2101 aataacgtag gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc
     2161 aaaggcaaat acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat
     2221 gttgcggatg gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa
     2281 gctaccaatg acgatgtcga agccaacacc accaatatca ccaacctgac ggattccgtt
     2341 ggtgaccttc aggctgatgc cctgctctgg aacgaaacta aaaaggcatt cagtgcagct
     2401 cacggccagg ataccaccag caaaatcacc aacgttaaag atgccgacct gacggctgac
     2461 agcactgatg ctgttaacgg ctctcagctg aaaaccacca acgatgctgt ggcgacgaat
     2521 accaccaata tcgccaataa cacttccaat attgccacta acaccaccaa catctctaac
     2581 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
     2641 gtattcacgg cagctcatgg caccgagacc accagcaaaa taaccaacgt taaagatggc
     2701 gacctgacga ctggcagcac cgatgccgtt aacggctctc agctgaaaac caccaacgat
     2761 gccgtggcga cgaataccac caatatcgcc actaacacca ccaacatctc taatctgact
     2821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtcttc
     2881 actgcagctc atggcaacaa taccgccagc aaaatcacca atatcctgga cggcacagtc
     2941 actgcaacca gttccgatgc cattaacggt agccagcttt atgacttaag cagcaatatc
     3001 gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac cggtccaacc
     3061 tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc gattaactcc
     3121 tcatttagca cgtctctcgg cgatgctctg ctttgggatg ccaccgcagg taaattcagt
     3181 gccaaacacg gtactaatgg tgacgcaagc gtgatcactg atgtcgcaga tggtgaaatt
     3241 tcagactcca gttctgacgc agtaaacggc tcacaactcc acggcgtgag cagttatgtt
     3301 gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg gcaccatcac tgcgccgacg
     3361 tacaccattg ctaatgctga ttacgataat gtcggtgatg ccctgaatgc tatcgatacc
     3421 actcctgacg acgctctgct ctgggatgcg gacgccggtg aaaatggtgc atttagcgcc
     3481 gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcaatctct
     3541 gctgccagca gcgacgcgat taacggctca caactctata ccaccaataa gtacatcgct
     3601 gatgcgctgg gtggtgacgc agaagtcaac gctgacggca ccatcaccgc accgacttac
     3661 accattgcga acgccgagta caacaacgtc ggtgacgccc tggatgcgct tgatgataac
     3721 gccctgctgt gggataagac tgccaatggc ggtgctggag cctacaatgc cagccatgac
     3781 ggtaaagcca gcatcatcac taatgtcgct aatggcagta ttagtgagga cagtaccgat
     3841 gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa cacccaaatt
     3901 atcaatcagc tcgctggtaa caccgacgca acctatatcg aagaaaatgg cgctggtatc
     3961 aactacgttc gtactaacga caacgattta gcctttaacg atgcaagcgc ctctggtgtc
     4021 ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg ccagcagcgt agccattggt
     4081 cagaacagca gcagcaccgt tgataccggt attgcgctgg gtagcagctc cgtttccagc
     4141 cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg aaaatggcgt ggtgattggt
     4201 tatgacacca ctgacggcga actgctaggt gcattgtcaa ttggtgatga cggtaaatac
     4261 cgccaaatca tcaacgtagc tgatggttca gaagcccatg acgccgttac ggttcgccag
     4321 ttgcagaacg ctattggagc ggtcgccact acgccaacca agtactttca cgccaactca
     4381 acggcagaag actcactggc cgttggtgaa gactcgctgg caatgggtgc gaaaactgtc
     4441 gttaatggta atgcaggtat tggtatcggt ttgaacactc tggttctggc tgatgcgatc
     4501 aacggcattg ctatcggcag taacgcacgc gcaaaccatg caaacagtat cgcaatgggt
     4561 aacggttctc agaccacccg tggtgcacag actggctaca ccgcctacaa catggacgca
     4621 ccgcagaact ctgtaggtga gttttctgtc ggtagtgaag acggtcaacg tcagatcaca
     4681 aacgtcgcag ctggttcagc ggataccgat gcggttaacg tgggtcagtt gaaagtcact
     4741 gatgagcgcg tagcgcaaaa tacccagagc attactaacc tgaacaatca ggtcactaat
     4801 ctggatactc gcgttactaa tatcgaaaac ggtattggcg acattgtcac caccggtagc
     4861 accaagtact tcaagaccaa caccgatggc gtagatgcca acgcccaggg taaagatagc
     4921 gttgctattg gttctggttc cattgctgcc gctgacaaca gcgtcgcact gggtaccggt
     4981 tccgttgcag aggaagaaaa tacaatctct gtaggttctt ctactaacca acgccgtatc
     5041 accaacgtag ctgccagtgt taatgccacc gatgcggtta acgtgtcgca gctgaaatct
     5101 tctgaagcag gcggagtgcg ctacgacacc aaagctgatg gttctatcga ctatagcaat
     5161 atcaccctcg gtggtggcaa cggcagtacg actcgtatca gcaacgtctc cgctggcgtc
     5221 aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa gcgcgcagga aacgaagcaa
     5281 tacaccgatc agcggatggt tgagatggat aacaaactgt ctaaaactga aagcaagttg
     5341 agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta tacaccgggt
     5401 gccagcatgg cttctattgg tggcggtact tacaacggtg aatcggcagt tgctttaggt
     5461 gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc
     5521 cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc cattaagtta
     5581 gtgtgactaa gggcaaacgc caaattgcct gatgcgttac gcgcatcagg caagataagg
     5641 cgctccgggt caacaatcta agggcaattc tctgatgagg attgcccttt tctttaccag
     5701 acatctcccc ccacaagaat tggccctacc aattcttcgc ttatctgacc tctggttcac
     5761 aattccccaa ttaaaactca catcaatatt gccaatacat aacatttagt taaccattca
     5821 ttgtcattat ccctacacaa cacaattggc agtgccactt ttacacaacg tgtgacaagg
     5881 agatgagcaa cagactcatt acacgatgtg cgtggactct aggagacctg caatgaatct
     5941 ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagtctga tagcatcgct
     6001 tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc
     6061 ctcatggacg gtagcaatcg cccttgccgt ggctttgctg ttctataaaa tgccggtcgc
     6121 taacgcgctg gcctcggtgg tttatggctt cttctatggt ttgtggccca tcgcgtggat
     6181 cattattgca gcagtgttcg tctataagat ctcggtgaaa accgggcagt ttgacatcat
     6241 tcgctcgtct attctttcga taacccctga ccagcgtctg caaatgctga tcgtcggttt
     6301 ctgtttcggc gctttccttg aaggagccgc aggctttggc gcaccggtag caattaccgc
     6361 cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc gccggactgt gcctgattgt
     6421 taacaccgcg ccagtggcat ttggcgcgat gggcattcca atcctggttg ccggacaagt
     6481 aacaggcatc gacagctttg agattggcca gatggtgggg cgtcagctac cgtttatgac
     6541 cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta tcaaagagac
     6601 gtggcctgcg gtcgtggttg caggcggctc gtttgccatc gctcagtacc ttagctctaa
     6661 cttcattggg ccggagctgc cggacattat ctcttcgctg gtatcactgc tctgcctgac
     6721 gctgttcctc aaacgctggc aaccagttcg cgtattccgc ttcggtgact tgggggcgtc
     6781 acaggttgat atgacgctgg cccacaccgg ttacaccgcg ggtcaggtgt tacgtgcctg
     6841 gacaccgttc ctgttcctga ccgctaccgt aacactgtgg agcatcccgc cgtttaaagc
     6901 cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc aacattccgg tgccgtacct
     6961 caataaactg gttgcccgta tgccaccagt ggtcagcgag gctacagcct atgccgccgt
     7021 gtttaagttt gactggttct ctgccaccgg caccgccatt ctgtttgctg cactgctctc
     7081 gattgtctgg ctgaagatga aaccgtctga cgctatcagc accttcggca gcacgctgaa
     7141 agaactggct ctacccatct actccatcgg catggtgctg gcgttcgcct ttatctcgaa
     7201 ctattccgga ctgtcatcaa cactggcact ggcactggcg cacaccggtc atgcattcac
     7261 cttcttctcg ccgttcctcg gctggctggg ggtattcctg accgggtcgg atacctcatc
     7321 taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg tctctgatct
     7381 gttgctggtt gccgccaata ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc
     7441 tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt
     7501 tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca cgcttcaggc
     7561 ttatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct gtcagacgag
     7621 gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg catgaagttg
     7681 cccgctgagc gccaactggc gatgcaactc ggcgtatcac gtaattcact acgcgaggcg
     7741 ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac gcggcggcgg gacgtttatt
     7801 cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctaaa aacgctgatg
     7861 gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat tgaagccagc
     7921 accgcatggc atgcggcaat gcgcgccaca cctggcgaca aagaaaagat tcagctttgc
     7981 tttgaagcaa cgctaagtga agacccggat atcgcctcac aagcggacgt tcgttttcat
     8041 ctggcgattg ccgaagcctc acataacatc gtgctgctgc aaaccatgcg cggtttcttc
     8101 gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga tgtatctggt gccaccggtt
     8161 ttttcacaac tgaccgaaca acatcaggct gtcattgacg ccatttttgc tggtgatgcc
     8221 gacggggcgc gtaaagcaat gatggcgcac cttagttttg ttcacaccac catgaaacga
     8281 ttcgatgaag atcaggctcg ccacgcacgg attacccgcc tgcccggtga gcataatgag
     8341 cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc gccgcagcgc
     8401 aacgcattct gccgccgttc ctgttccact atatggatgg tggtgcatat tctgaataca
     8461 cgctgcgccg caacgtggaa gatttgtcag aagtggcact gcgccagcgt attctgaaaa
     8521 acatgtccga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg atgccggtag
     8581 cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt caggcagcca
     8641 aagcggcgga cgcgcatggc attccgttta ctctctcgac ggtttccgtt tgcccgattg
     8701 aagaagtcgc gccagccatc aagcgcccaa tgtggttcca gctttatgta ctgcgcgatc
     8761 gcggctttat gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg acgctggttt
     8821 tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga tgcgcattca ggtatgagcg
     8881 gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa tgggcgtggg
     8941 atgtgggcct gaacggtcgt ccacatgatt taggtaatat ctcagcttat ctcggcaaac
     9001 cgaccggact ggaagattac atcggctggc tggggaataa cttcgatccg tccatctcat
     9061 ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatc aaagggatcc
     9121 tcgatccgga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt gtggtttcta
     9181 accacggtgg ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg cccgctattg
     9241 cagatgcggt gaaaggtgat atcgccattc tggcggatag cggaattcgt aacgggcttg
     9301 atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact gctgggtcgt gctttcctgt
     9361 atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg atcgaaaaag
     9421 agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat cagtgaaatt acgcaagatt
     9481 cgctggtgca ggggctgggt aaagagttgc ctacggcact ggctccgatg gcgaaaggga
     9541 atgcggcata gtcgtttgcg ccccctcacc ctaaccctct ccctcaggga gaggggaccg
     9601 ttcggcgctg tatgtactcc ctcactctga aacgacaccg cactcttttt ttctccctcg
     9661 cccctccggg gagggccggg gtgaggggaa aaggccgcac ggttcaccaa ttttctgcta
     9721 tcctgccccc gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc
     9781 cgccaaatac tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctacatatca
     9841 tcgaaccgat gggatttgcc tgggacgaca agcgcctgcg ccgcgcgggg ctggactatc
     9901 acgagtttac cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc
     9961 cccagcgcct gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc
    10021 aggatggcga ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg
    10081 atgccctgcc cgctgaacaa aaaattcgca ttccgatggt gccggacagc cgcagcatga
    10141 atctgtccaa tgcggtgtcg gtagtggtgt atgaagcctg gcggcagctg ggatatccgg
    10201 gagcgttgtt gagagattag ttactgtatg ccggatagat gattacatcg catccggcac
    10261 gatcacagga cattagatcc catccccata ctcaaatgta tggttaatac cgttgaaatg
    10321 ctggtccata tccattgatg gcttatcgct gtctggttta ccgacaatac gagccggaac
    10381 gccagcggcg gttgtatgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt
    10441 cgcgccgcga ccaacttcaa tattgccgag gattttcgcg cccgcgccaa tcatcacacc
    10501 ttcacgaatt ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga
    10561 ttgcagaatc gatacgtcgt tttcaattac cgccgtttca cccacaacga tgcccgtggc
    10621 gtggtcgagc ataatgccac ggccaatttt tgctgccggg tgaatatcga cctggaacgt
    10681 cacagaaacc tggttttgca gaaagattgc caatgcgcga cgcccctgat tccacaacca
    10741 gtgaccgatg cgataggcct gcaaggcatg aaaacccttc aggtataaca acggggttga
    10801 gtatttatcg actgccgggt cgcgggtacg caccgcctga atatcacagg ccgcagaggc
    10861 gatcatttcc gggtcagcgg cgtaggcttc ttcaaccact tcacggatag caatagcagg
    10921 cataattggc gatgacagct tgttcgccag catgtagctc agtgcactgc caaggttttc
    10981 gtgcttgagt agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt
    11041 tctggcttcg gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta
    11101 ctccacacga tgagataatg accggcccgt tctgcgcggg ccgggtcata gcggtaacaa
    11161 aggttccctg gggttagtga ctgctgcgct cgtccttgcg tgcacgacct aataatgtca
    11221 atgctgcctc gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg
    11281 gcatttcaac gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt
    11341 ccaccacctg accaatcttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca
    11401 taccaaaacg gcggttacgc gactggttgt cggtacaggt aagcaccaga tcgcccagcc
    11461 ccgccatgcc cataaaggtg gcaggatcgg cacccagcgc cgcaccaaga cgcgacattt
    11521 cagccagccc acgggtgatc agcgccgtac gcgcattcgc accaaaaccg ataccgtcag
    11581 acatccccgc gccaatggca atgacgtttt tcaccgcgcc gccaagctgc acgccaatga
    11641 aatccgggtt gctgtaaacg cggaaacttt tgccgcagtg cagcagctgc tggagatcat
    11701 cggcaaaggt ctgatcggtc gaggccagcg aaatagctgt cggtaaacct gccgccagtt
    11761 ctttcgcaaa cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac
    11821 gcgccacgtc ctgtaacaga cgtccggttt ccgcttccag ccctttggtc gcccacacca
    11881 gacgcgcatc aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc
    11941 tgggtacgac gacgagaata ttacgactgg ctgccagcgc agtggcgaga tcgctttcaa
    12001 gatggagcgt atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt
    12061 caagcgttgc gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg
    12121 ccagggtaat ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat
    12181 tacgttggtt catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa
    12241 atagttcatg aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc
    12301 gcgggatacc atgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta
    12361 tgcgcccagg caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc
    12421 ctgctgaact tcacacagga acgcggtttc ttcgcccaag gaggccgtta cggtaacacg
    12481 cagtaccact tcgtatacgt catctgccag ttgggaagat gccgtatcca gatcaagttt
    12541 aacttctggt tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagagat
    12601 atccttggta taaatacgtt ggatctggaa agtcatttca gtgttgtttt gttctgacat
    12661 gtgtagaaaa cccttaagtg ttgtccttaa atactgcgca atgtaatacc aacgttcaca
    12721 cgttatttca gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag
    12781 ccgccaatgt gctgtgcgtc aataaaaatc tggggaaccg tggtgcaacc gctgcgtttg
    12841 atcatctctt cacgcttggc ggcattgcca tcgatcggca actcctggaa actcacgccc
    12901 ttgctgctca gcagtgcttt tgcacgatgg caatacgggc aggtttcttt ggtatagatt
    12961 tcaacattgg ccataattta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg
    13021 gctgcaaaat ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca
    13081 gctaacggcc tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggcaag
    13141 ttttcgccag cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct
    13201 ttcgtcagcg cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc
    13261 gatttgtctt tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac
    13321 agattgatag aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca
    13381 accacagcgt cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact
    13441 ttcgaggtca ggctcttaaa cgtagtcaca agaaccgcca ccagtaacgc gatccaggcg
    13501 atactcagta tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac
    13561 tcccgacgta gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cggcaaatac
    13621 agccagcgcg ttaactggaa tgcaattttg cggggcacga cgaaaaaaaa gcgcaaatct
    13681 ggcatcggct tgccgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc
    13741 agctgtagta aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt
    13801 tctaaaaaac ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag
    13861 gataacgcca tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg
    13921 cataccctga tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac
    13981 tccgaagtag gccacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt
    14041 ctggacgttg aaatcaaaga tcgcgctttc tttgctaatc cagtgctggc tggcgcggta
    14101 cataaagcga aaaacgcagg taaagcagta cacattatgg gtctgctctc cgcaggcggc
    14161 gtacacagcc acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca
    14221 gaaaaaatct acctgcacgc attccttgac ggtcgcgaca ccccgccgcg cagtgctgaa
    14281 tcctcgctga aaaaatttga agagaaattt gctgcgctgg gtaaaggccg cgtagcgtcc
    14341 atcattggtc gttactacgc gatggaccgc gataaccgtt gggatcgcgt tgaaaaagcc
    14401 tatgacctgc tgactctggc gcagggtgag ttccaggccg atactgccgt tgctggtttg
    14461 caggcagctt atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca
    14521 gaaggtcagc cagatgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt
    14581 gctgaccgcg cgcgtgaaat cactcgtgca ttcgtgaacg ctgatttcga tggcttcgcg
    14641 cgtaagaaag tggttaacgt cgatttcgtg atgctgaccg aatacgccgc tgacatcaaa
    14701 actgcggttg cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa
    14761 aacgacaaaa ctcagttgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc
    14821 ttcaacggtg gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg
    14881 aaagtggcta cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg
    14941 gttgcggcca tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac
    15001 atggtaggtc acaccggggt gatggaagcg gcggttaaag cggttgaagc actggatcac
    15061 tgcgtggaag aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct
    15121 gaccacggta acgctgaaca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacac
    15181 accaacctgc cagttccgct gatttacgtt ggtgataaga acgtgaaagc ggttgcaggc
    15241 ggcaaacttt ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa
    15301 gagatgactg gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg
    15361 attaatacca tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac
    15421 gccagcgttc ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt
    15481 gaccaactca aatctattca ggccgatatc gccgcaaaag agcgcgcggt acgccaaaag
    15541 caacaacaac gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa
    15601 gccacccgta agctgcgcga aacgcaaaac acgctcaatc aactcaataa acagattgat
    15661 gagatgaacg cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc
    15721 gccgcacaac tggatgccgc attccgtcag ggcgagcata ccggtattca gctaattctc
    15781 agcggtgaag aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag
    15841 gcgcgacaag aaaccattgc ccagttgaag caaacgcgtg aagaagtcac catgcagcat
    15901 gctgaactgg aagagaaaca aagcgagcaa caaacgctgt tatatgaaca gcgcgcccaa
    15961 caggcgaaac tgactcaggc tctgaacgag cgtaaaaaga cgctggcggg gctggaatct
    16021 tccatccagc aaggtcagca gcagctaagt gaactgcgtg ccaacgaatc ccgtctgcgt
    16081 aacagcattg cccgtgctga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag
    16141 gcccaggcgg ttcgcgagcg ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg
    16201 accgaaagcg aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag
    16261 gcattctggc cggttcgcgg gccgacgctg catcgctatg gcgagcagct acagggtgaa
    16321 ttacgctgga aagggatggt tatcggtgcc tctgaaggta ctgaagttaa agcaattgcc
    16381 gacggtcgag tgattctggc tgactggctg caaggttacg gtctggtggt ggtggttgag
    16441 cacggtaaag gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt
    16501 tcgcaggttc gcgcgggcca gccaattgca ctggtgggca gcagtggcgg tcagggtcgg
    16561 ccttcactct atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg
    16621 ggaagataag ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg
    16681 ctctcctccc ccgtacttgc tggcaaactt gccatcgtca ttgatgattt tgggtatcgc
    16741 ccgcacaacg aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc
    16801 gattcaccgc acgccagaga gatggcgacc aaagcgcata acagcggtca cgaagtgttg
    16861 attcatctcc cgatggcacc gttgagtaaa cagccgctgg agaaaaatac gctacgcccg
    16921 gagatgagca gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat
    16981 gccgtgggga tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag
    17041 aaagtgatgc aggcgctgga gcgttacaat ctttacttcc tcgacagcgt aaccatcggt
    17101 aatacccagg cgatgcgcgc cgcgcaaggt actggcgtga aggtgatcaa acggaaggtg
    17161 ttcctcgacg attcgcagaa cgaagcggac atccgtgtgc aatttaatcg cgcaattgat
    17221 ctggcgcgtc gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc
    17281 gtgctacaac agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc
    17341 ttgctgaatg aaccgcaggt tgatacttct acaccgccga aaaacgctgt gcctgatgca
    17401 ccgcgtaatc cgttccgtgg cgtgaagctg tgcaaaccga agaaaccgat agaacctgtt
    17461 tatgctaatc gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt
    17521 tacttccagc atcagtggca aggctggggc aaacagcccg aagcggcgaa gtttaacgct
    17581 agcgcaaatt aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt
    17641 gccataagcg gaaagaccac agtaacgcct ggtaaccgac cttcacgctg cgcacattgg
    17701 taatcaggcg cttatacata cccggaagta aatatctcgg caatcatccg ttggcgggta
    17761 agaatatccg gctctttgcg
//
LOCUS       DAZSNG010000082        17732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZSNG010000082 DAZSNG010000000
VERSION     DAZSNG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17732
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.083"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            145..747
                     /gene="ais"
                     /locus_tag="ACHW4Z_002848"
     CDS             145..747
                     /gene="ais"
                     /locus_tag="ACHW4Z_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR8882413.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(786..1211)
                     /gene="nudI"
                     /locus_tag="ACHW4Z_002849"
     CDS             complement(786..1211)
                     /gene="nudI"
                     /locus_tag="ACHW4Z_002849"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR8882414.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1490..2032
                     /locus_tag="ACHW4Z_002850"
     CDS             1490..2032
                     /locus_tag="ACHW4Z_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR8882415.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            2119..3321
                     /locus_tag="ACHW4Z_002851"
     CDS             2119..3321
                     /locus_tag="ACHW4Z_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR8882416.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3385..3846
                     /locus_tag="ACHW4Z_002852"
                     /pseudo
     CDS             3385..3846
                     /locus_tag="ACHW4Z_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3887..4837)
                     /locus_tag="ACHW4Z_002853"
     CDS             complement(3887..4837)
                     /locus_tag="ACHW4Z_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR8882417.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5030..6220)
                     /gene="glpC"
                     /locus_tag="ACHW4Z_002854"
     CDS             complement(5030..6220)
                     /gene="glpC"
                     /locus_tag="ACHW4Z_002854"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR8882418.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6217..7476)
                     /gene="glpB"
                     /locus_tag="ACHW4Z_002855"
     CDS             complement(6217..7476)
                     /gene="glpB"
                     /locus_tag="ACHW4Z_002855"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR8882419.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7466..9094)
                     /gene="glpA"
                     /locus_tag="ACHW4Z_002856"
     CDS             complement(7466..9094)
                     /gene="glpA"
                     /locus_tag="ACHW4Z_002856"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR8882420.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9367..10725
                     /gene="glpT"
                     /locus_tag="ACHW4Z_002857"
     CDS             9367..10725
                     /gene="glpT"
                     /locus_tag="ACHW4Z_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR8882421.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10730..11806
                     /gene="glpQ"
                     /locus_tag="ACHW4Z_002858"
     CDS             10730..11806
                     /gene="glpQ"
                     /locus_tag="ACHW4Z_002858"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8882422.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11848..12090)
                     /locus_tag="ACHW4Z_002859"
                     /pseudo
     CDS             complement(11848..12090)
                     /locus_tag="ACHW4Z_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12085..12189
                     /locus_tag="ACHW4Z_002860"
                     /pseudo
     CDS             12085..12189
                     /locus_tag="ACHW4Z_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12269..12919
                     /gene="inaA"
                     /locus_tag="ACHW4Z_002861"
     CDS             12269..12919
                     /gene="inaA"
                     /locus_tag="ACHW4Z_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR8882423.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12973..13227)
                     /gene="yfaE"
                     /locus_tag="ACHW4Z_002862"
     CDS             complement(12973..13227)
                     /gene="yfaE"
                     /locus_tag="ACHW4Z_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR8882424.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(13227..14357)
                     /gene="nrdB"
                     /locus_tag="ACHW4Z_002863"
     CDS             complement(13227..14357)
                     /gene="nrdB"
                     /locus_tag="ACHW4Z_002863"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8882425.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14591..16876)
                     /gene="nrdA"
                     /locus_tag="ACHW4Z_002864"
     CDS             complement(14591..16876)
                     /gene="nrdA"
                     /locus_tag="ACHW4Z_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8882426.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            17004..17147
                     /gene="ypaB"
                     /locus_tag="ACHW4Z_002865"
     CDS             17004..17147
                     /gene="ypaB"
                     /locus_tag="ACHW4Z_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR8882427.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4209 a         4297 c         4705 g         4521 t
ORIGIN      
        1 taggctttgc atttggcaaa aatttgaggc attttgccga catcgtagga tttttaatat
       61 tacaccaact gctaattatc gccagaaatg tcgctcaatt tcacggtaat tgtctggttg
      121 cgcttgtcta taggtggagt ttacgtgtta gctttttgcc gctcatcatt gaagtcaaaa
      181 aaatatttca tcattttact ggcgctcgct gcaattgccg gactgggtac tcatgccgcc
      241 tggagtagca atggtttgcc acgtatcgac aataaaacat tggccagact ggcacagcag
      301 cacccggttg tcgttttgtt tcgccatgct gaacgttgcg atcgttcaac caatcaatgc
      361 ttgtcagata aaacaggtat tacggttaaa ggtacccagg atgcccgtga actgggcaac
      421 gcttttagcg ctgacatctc tgatttcgat ctttattcca gtaataccgt ccggaccatt
      481 cagtcggcga cctggttttc cgcgggtaaa aagctgacgg tagataaacg acttcttcag
      541 tgcggtaatg agatttatag tgcaattaag gacttacaaa gcaaagcgcc tgataaaaat
      601 atcgttattt tcacccataa tcattgcctg acatatattg ctaaaaataa gcgtgatgcg
      661 atatttaaac ctgattatct ggatggttta gtcatgcatg tggaaaaagg caaagtttat
      721 ctggatgggg aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat
      781 cattgttaaa gaagaccttt caaacgtaac gtttttcggg tggcaacatt taaatcataa
      841 tgcaccagat cttcaggttt tacccacgcg tagtcctgaa actcttcgtt tattttcact
      901 tctcggttgg cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga
      961 ccatctgcat atgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc
     1021 aaaagcagct gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt
     1081 tcgccagact ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg
     1141 tcggccattt tgcacagcaa ataagcacca tcattttgaa tcaaagggca tacaatagtc
     1201 cgttgtcgca caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca
     1261 gaaatatgtg aaagtgacag taatctcttt tgcaatgata actgcactta ttattatttt
     1321 tgtgtgtatc ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag
     1381 ttacaataag tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca
     1441 atcccgcagc tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat
     1501 tgcgctagca ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat
     1561 cagcggccag gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac
     1621 gggcctggct ttaagcggta actggacaca taacgacgac gacggtgacg tcgcgggcgt
     1681 ggggctgggg ttgaatctgc ctctcgggcc gttaatggcg accgttggcg gaaaaggcgt
     1741 gtacaccaac ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca
     1801 gtggaaaatt ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcgct
     1861 ctccagcggt attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg
     1921 tccagtcagt attgaggcgg gttatcgcta cctgaatctg tcgggtaaag acggtaaccg
     1981 cgacaacgct gtggctgacg gcctgtatgt tggggttaac gccagtttct gatcccttcc
     2041 ggcgcggcat tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa
     2101 caaaagcagg agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta
     2161 tacgggcaaa tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg
     2221 ctgccattat ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt
     2281 cgcgaacgta gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc
     2341 gatgatttaa gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa
     2401 gcctggctca aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc
     2461 aaccgtaaac aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc
     2521 gcctgtggtt ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg
     2581 tcagaattta aggtgatggt cgagcacgaa atcctgccgc gcctgcgcga gcgtttttct
     2641 ttaccacagc cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg
     2701 gcacaaagcc tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcctca
     2761 atgccgatca tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa
     2821 ctgtggctgg acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga
     2881 ctgcccgcgc agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag
     2941 caattcaccg gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg
     3001 tgtgaagtga ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa
     3061 cggcgggcca accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat
     3121 ctcaactttg cgctagccac gccagacggc actttcgctc tgcgtgtgcg tttcagcact
     3181 acacgctaca gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgcta
     3241 cgccgttggt taaacggcca ggacatcgct agtgagcatg gctggattga ggttgttgag
     3301 tccatgactt tatccgtctg atttcgccac tctgtgccgg tttactgcac cggcacattc
     3361 cccctccggc aaaatttgtc caaccctccc tacggtgagc gtggtgttgg ggccagtgtg
     3421 gcacgggctg cgcgctgggg acgcattgag aattacatgg cgcaagtcaa cgattcgctt
     3481 tgtctgttag tgcaggtgga aagtaaaacg gcactggata acctggacga gatcctcgac
     3541 gtcgaaggga ttgatggcgt gtttattgga ccagcggatc tttctgcgtc gttgggctac
     3601 ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc
     3661 cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc
     3721 ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc
     3781 ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtagt
     3841 tattgatatc aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg
     3901 ctgccagatc ggcttcgctt agctgggtgg ccgcgagcac ttggtcacga tcaatgccct
     3961 gttccagcat gcgcaaagca attttaatgg cttgttcatg cataccttcc tgcaaacctt
     4021 ctaatttacc ttgctgcaaa cctactaatt taccttcttg ccagccaatt tgatgtcctt
     4081 cctgccgtag ccgttcagca atagtcatta atctctcctt ttgtagtggt gaacgttcgg
     4141 caatctcctg aataaaacgg gtgaaacggg aggtatcgcc gcattgcagc agataattaa
     4201 acagtgtttg tagctggctg tcattagtga agcctctaac caaaagcgtg gtgatcctgt
     4261 cgaccatgcc gattaaatcg tggtcgcgaa tatgcttttg aatcagttcc agcagagcta
     4321 tacgccgatg ttgcatgatc tcatcgtcag gtacgatggt gatatccacc aacggaaaag
     4381 cttcggtgta caactgccgg gcaagttgcg gatcgtcaaa ctcatccagc cagttaagtg
     4441 agtacgggta gggcgatgtt tcgccatgat aaaacagcaa tggcaccacc agcggaactc
     4501 tgtcatagcc tttatctagg tgactctgca tggcggcagt ggcatagcgc attggccgaa
     4561 aagccatatt cttttctgca gagctttgat gttcaatcac gcagtagata tagccgtcac
     4621 cgtcgctggt ttccacggac cacaaaacat ccgagtagta agcgcgtaaa cttttttcaa
     4681 taaaactagt gggttccagg cgtaaggttt gcaggttgca aagcttgcgc agtggttctg
     4741 gtaaatgtat ttcgagaaaa tcccgtgcgg tttcgggtgt gaatataaag gttttaaata
     4801 ccgcatcatg cggggaggag gttgttgatt cggtcatggt tgtccgtcac ctgatattta
     4861 tgatgacgcg acattacggt tatcccaggc ctttgacatc tgccagtttc agcctttgcg
     4921 agacgttttc agaggaattt tcactgcgtt gcaacgcata cattgggcac ggaatcgtgt
     4981 acgcaaaaat gcccggtaac cacatgctta ccgggcatca gaagggagtt taagccagcg
     5041 cctgggccag tagcgtaatc ggatgttcgc agcgaagact tgtggacatc tctatctgcc
     5101 atttacaggt ttcgcagtcg gtgaccacca gatctgcgcc gctttcttct atctggcgga
     5161 acagtggtgc gccgatggct tgtgaggtgg ggtagttctc ttttttgaaa ccgtaagtac
     5221 ccgcaatacc gcagcactgg gaatccagca ccgttaactc aagccccggg atgttacgca
     5281 acagctccag ggtgtagagc gtccagccca ttttttccat atggcacgga gtgtgataaa
     5341 ccactttcag cggcagcggt ttcagcggta acgttttgcc ttcgtccagc ttgcgccaca
     5401 gccagcgggt tgccagttcg atatgatcgc gcaagccttt gttgtcgaca ttcagcactt
     5461 ccgggtattc gtcgcgcagg gcaaatgtac aggttgagga ggtggcaatc accggaatgc
     5521 cttttactcc cacagcttcg cggatcgact ctacattcgt aattgcctgt ttgcgtgctt
     5581 tatcggtaaa gccgttggcg attagcggta cgccgcagca tttttctttg ctgagcagtt
     5641 gtacaccggt acccattgcg ttgagcactt taattaaatc tttacctaac tgcggatggt
     5701 tgtagttaac gaagcagccg tgaaagaaag cgacctggtc tttatattgt gcttgctgag
     5761 ccgctatgct gcgataccag cgacggaacg tgccgaagga gtatttcggt agcgtgcgac
     5821 gatgatcgat ttttaacgcc gcatcaagca gctgccgcac cggtttcagc gaggtagcgg
     5881 tgttgacgat tggtgcgaac ggcgtggaaa cgctacccat caggtcggta tgactcaaca
     5941 caaaattacg cagcgacggg cgcgtggtgt catatttcgc ccgcgcgcgc tggataatat
     6001 cgccaatctt cacatcggac gggcaggcga cttcacaacg tttgcagttg atgcaatatt
     6061 tcagcgcctc gtcatacagt gcgccatctt tcaaacgcag acgctcgcca tccggcccgg
     6121 cttgttttgg ccctggataa ccgggattca cccggctcac cgggcaggcg gtggtgcaga
     6181 cggtgcactt aatgcagttt tcgaagctgg tgtcgttcat tgttggcctc ctgcgcgttg
     6241 ggcaatctgt tgagcggcat gtaaagcact gacggcacaa acaccgccgc cgcatccctg
     6301 ggcgatggga tcaaatccgc ccagcaccga accgatggca aacaggttct caatggtttg
     6361 ccctgcctgc gacgggcgta gcgtctcatc agtggttaca ccgaactgct gccacggttg
     6421 cggcgcaaaa aaatcgccct tataccattc accccgcgtg gcggtttgta gcacatcaag
     6481 gccgagaatc ggctcgcgaa tgccgttacg ttccgctact agtccgccac taaagaaact
     6541 gccgctggcg agaaccgcga aacgtggacg tagcggaata tcggcgtgat tgcgggtcca
     6601 gatttcattc actacgccat ttttacaggt cacttttttc acttcatcgc ccggcatcca
     6661 cactccacct tggcgcacaa actggcgctg taactggttt tgcagacgaa tgcccagcac
     6721 ggaaggcggc agcgttggca aaagcatcag tgaacaaggt agtttttcat tcaaccaacg
     6781 ccacagtttg tcatcggcca ggccgaagca ggcgggcatc aggatcattt cgcaggtatt
     6841 ggcgacagga ataagcgcat caagtaacag cggccagttt tcttcattat caaggaaacg
     6901 ggcgatattc accgcgcgaa attcggtggc gttattgcgc aggacatcca gttccggcag
     6961 ctctatttct gcggtttcaa ccgaaagatc cagttcgcgc aacgaagccg ccgcaagatg
     7021 cgcctggaaa tccatcagcc cgcttatccc cactacacat atcttcttcg cgggtagcgg
     7081 ccagacggga acttctggcg aacttaacca ggttgcacgc aaagtgccga gcggcgtaat
     7141 ccgctggtga gccagttcta cgctgccctg caattgcgca ccgctttcag cgattaatgc
     7201 ctgcgcctgg caagcgagat cgagcacgcg ttgtggcccg agaagagagt aaggatgtgc
     7261 aggtgcctgc tgacgcaaag attccagtcc actgtgaatg tctgccacgg gttgaccatc
     7321 tggcagatgg ctcagcaaat ccagcgaacc ggatgagaaa tgcaacgcgc tttgaccacg
     7381 cgtgacaatg gcacagcgca ggccgtgttt ttgcagttgc aggccacaga gtaatccggc
     7441 gaggccgccg cccataatga cagtatcaaa gcgcatcttt ctgctccttc tccagaccac
     7501 acagtccctg ataaacccag cgggtaaatt cgctttcgcg cagtgcgtct ccccaggcga
     7561 tgggttgcac gcctttccag cgttcgttaa ggaaggtgga aagttgctcg atagattgcg
     7621 cggacgtcgt gacgttaaac cgttgcagca gtccggcagc gcggcaggcg cagagttcgc
     7681 cctggcaggt gcccatcccc acacgggtac gacggcgtaa atccagcagg ctattaacgt
     7741 ttaaattttc taccgcgtac tgcacttcac ccgcagttac cgcttcgcac tcacatacca
     7801 ggctacggtg tagacggcct tcgctcagcc aggcaggcgt gcgatcgcca tgacgataaa
     7861 ccgcagaacc gcgcagcggg gcaggcaggg agatgacttt acgcaaggta acttcagcgg
     7921 gttcttgtga accaggcagt gccagatcgg cagtcgtaca ggggcgcgtg ttgcccagtt
     7981 tgcggcatac cgcgtcggta gcccattcag ccatcagccg ataggtcatc agtttgccac
     8041 cggtgatggt gataaatccg tccagaccat cgcgttcagc atggtcgagc agcacgatgc
     8101 cacggctgac gttacgtccg ctcgggtcgt catcgctggc aaccagcggg cgcacgccag
     8161 aataggcccg caaaatgcgc gttttcgcca tcacgggggc cagtttttcc ccttcacgca
     8221 gcagaatatc aacctcttct gccgtcactc gattatcgtc aatctcgttg taatcaatac
     8281 gtaaagaggt ggtaccaatc agcgaaatgg tatcgccagg caccagaata tcggcgtcgg
     8341 aaggtttacg gcagcggttg atcacatgct ggttaatgcg gtgatccatg atcagcagcg
     8401 atcctttcgc cgggaacatg cgaatgcgca gatcggcata ttcggcaatg tgttgccccc
     8461 agatcccagc ggcattaacc acgacaggtg catgaagggc ctgagtttcg ccggtgagat
     8521 ggttacgtac acgaacaccg cacaccgtcg cgccttcacg aatcagcccc gtgacttcat
     8581 gagcggtaag gataacggca ccgtgttctt tggcatccag catgtttgct gcggtcagac
     8641 gaaatggatc aacggtgcca tccggaactt tcaccgcgcc aatcagtgcc gggttaacgg
     8701 caggttcgat aatgcgcgct tgctgcgggt ctatagcttc tgcgctgatc cctgcttctt
     8761 cgcaggcgcg aataaaagtg gcctggaatg agaggtcatc ttccggcagg gtgataaaca
     8821 ggccgttggt tggttcaacg cagtgacgtg caatgcgttt caggatctgg ttttcactaa
     8881 tgcattcgcg ggccgattcc gcatcggtta ctgcatagcg cgcaccgctg tgcagcaggc
     8941 cgtggttacg cccggtggca ccggttgcga tgtcgtggcg ctcaaccaaa atcacgcgca
     9001 gcccgcgcag ggcacagtcg cgggcaatcc cggctcccgt tgcgccgccg ccaatgataa
     9061 tcacgtcact tgattgcgag tcgcgagttt tcattgttat ccctctgaag ttcgtttttt
     9121 accatttagc catagtaaaa acatgaattg tttgatttcg cgcatattcg ctcatgattc
     9181 gaaagtaaaa cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg
     9241 tgtgcggcaa ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa
     9301 cggcaggttc tctcactgaa tcaggctgtt aatcataaat aagaccacgg gccacggagg
     9361 ctatcaatgt tgagtatttt taaaccagcg ccacacaaag cgcgcttacc tgccgcggag
     9421 atcgatccga cttatcgtcg attgcgctgg caaattttcc tggggatatt ctttggctat
     9481 gcggcttact atttggttcg taagaacttt gcgcttgcta tgccttatct ggttgagcag
     9541 ggattctcac gcggtgattt aggttttgcc ctttcgggga tctcgattgc ttatggattt
     9601 tcgaaattca tcatgggttc ggtatcggat cgctcgaatc cgcgcgtttt cctgcccgca
     9661 ggtttgattc tggcggcggc agtgatgttg tttatgggct ttgtgccatg ggcgacgtcg
     9721 agcattgcgg tgatgtttgt actgttgttc ctctgcggtt ggttccaggg gatggggtgg
     9781 ccgccgtgtg gtcgtactat ggtgcactgg tggtcgcaga aagaacgtgg cggcattgtg
     9841 tcagtgtgga actgtgcgca caacgtcggt ggtggtattc cgccgctgct gttcctgctg
     9901 gggatggcct ggttcaatga ctggcatgcg gcgctctata tgcctgcttt ctgcgccatt
     9961 ctggtggcat tattcgcctt tgcgatgatg cgcgataccc cgcaatcctg tggcttgccg
    10021 ccgatcgaag agtacaaaaa tgattatccg gacgactata acgaaaaagc ggaacaggag
    10081 ctgaccgcga agcaaatctt catgcagtac gtgctgccga acaaactgct gtggtacatc
    10141 gccatcgcca acgtgttcgt ttatctgctg cgttacggca tcctcgactg gtcaccgact
    10201 tatctgaaag aggttaagca tttcgcgctg gataaatcct cctgggccta cttcttctat
    10261 gagtatgcgg gtattccggg caccctgctg tgcggctgga tgtcggacaa agtcttccgt
    10321 ggtaaccgtg gggcaaccgg cgtcttcttt atgacactgg tgaccatcgc gactatcgtt
    10381 tactggatga acccggcagg taacccaacc gtcgatatga tttgtatgat tgttatcggc
    10441 ttcctgatct acggtcctgt gatgctgatc ggtctgcatg cgctggaact ggcaccgaaa
    10501 aaagcggcag gtacggcagc gggctttacc gggctgtttg gttacctggg cggttcggtg
    10561 gcggcgagcg cgattgttgg ctacaccgtg gacttcttcg gctgggatgg cggctttatg
    10621 gtaatgattg gcggcagcat tctggcggtt atcttgttga ttgttgtgat gattggcgaa
    10681 aaacgtcgcc atgaacaatt actgcaaaaa cgcaacggag gctaatggca tgaaattgaa
    10741 gctgaaaaac cttagcatgg cgatcatgat gagcactata gtcatgggaa gcagtgcaat
    10801 ggcggcggac agcaacgaaa aaatagtcat cgcccatcgc ggtgccagtg gatatttgcc
    10861 ggagcatacg ctgccagcaa aagcgatggc gtatgcgcag ggagcggatt atctggaaca
    10921 ggatttggtg atgaccaaag acgaccatct ggttgttctg catgaccatt atctcgatcg
    10981 tgttactgat gttgccgatc gtttcccgga tcgggcgcgc aaagacggtc gttactacgc
    11041 gatagatttc acgctggatg aaattaagtc gctgaaattt accgaaggtt tcgatattga
    11101 aaacggtaaa aaagtacaga cttatccggg gcgtttccca atgggtaagt ccgacttccg
    11161 ggtgcacacc tttgaagaag agattgaatt tgttcagggg ttaaatcact ctaccgggaa
    11221 aaatatcggt atctatccag aaatcaaagc gccgtggttc catcatcagg aagggaagga
    11281 tattgcggca aaaacgctgg aagtgctgaa gaaatatggt tacaccggta aagacgataa
    11341 agtttatttg caatgttttg atgctgatga gctgaagcgt attaagaatg agctggaacc
    11401 caaaatgggc atggagctca atctggtaca gctgattgcc tataccgact ggaatgaaac
    11461 gcagcagaaa cagccggatg gaagctgggt taattacaac tacgactgga tgtttaagcc
    11521 gggagccatg aaacaggtgg cggaatatgc agatggtatt ggtccggatt accatatgtt
    11581 gattgaggag acatcgcagc cgggtaatat caaactcact ggcatggtgc aagatgccca
    11641 gcagaataaa ctggtagtgc atccttatac cgtgcggtca gataaactgc ctgaatacac
    11701 tcctgatgtg aatcagttat atgatgctct gtataacaaa gcgggtgtaa atgggctgtt
    11761 tactgatttc cctgataagg cagtaaaatt tcttaataaa gagtaatgtt actgcaggat
    11821 ggcgttcatg cgccatcctg tctcatatca aatttttcca atcgccacta attgttccat
    11881 gcacatttgt agtaaaaaca gtggactgcc ttctaccggc ttttctttag tcagcaacga
    11941 aatctgtcga tagaaggttg gttcgtgagg aacggaacac aattcacctg caatgctttt
    12001 cagcgttaac tctggttgta aggcgatgcc taatccctgt cgaataaaat tcatcgctgt
    12061 atccgactga ttaaattcac attttatctg ctgggcgcac tattagtggt agtggctggt
    12121 ggaatctatg cgctgcaaat gaagggcaaa agcggtgtca cacctgtagc ggcaaaagaa
    12181 attcattaat acgacacgtt tcattaagat tttcctcagg taaccaccta tagtcattgt
    12241 cgttaataaa aatgacaggc gatagggtat ggcagtttca gcaaagtacg acgaatttaa
    12301 tcactggtgg gcaacagagg gcgactgggt tgaagagcct aactatcgcc gtaatggtat
    12361 gagcggggtg caatgcgtcg agcgcaacgg caaaaagctg tatgtaaagc gcatgacgca
    12421 tcatctgttt cattcagtac gttatccgtt cggccgacca acgattgtcc gtgaggttgc
    12481 ggtaattaaa gaactggaac gggccggtgt catcgtgccg aagatcgttt ttggtgaagc
    12541 ggtgaaaatt gagggtgaat ggcgcgcgtt gctggtgact gaagatatgg cggggttcat
    12601 cagcattgct gactggtatg cccggcatgc agtatcgcct tattctgacg aagtacggca
    12661 agccatgttg aaagcagtgg cactggcgtt taagaaaatg catagcgtta atcgtcagca
    12721 tggctgttgc tatgttcgcc atatctatgt gaaaacagaa ggtaaggcag aagctggttt
    12781 tcttgatctg gagaaaagtc gtcgccgctt gcgtcgcgat aaagcgatta atcatgactt
    12841 ccgccagctg gaaaaatatc tggagccgat ccctaaagcg gactgggagc aggtgaaagc
    12901 gtattactac gccatgtaat cgttgtaggc cagataatac gcgtaagcgt cgtatctggc
    12961 gctagtaccc gatcacaact cgatttcaat atcgcctttt gcccggcaac aacagggcaa
    13021 aatttccccc ggctgaataa aggctaacgg ttcggcaatc cagtccacct gacctgcgac
    13081 cagtcgggtg cgacaggagc cgcagtaacc ttcgcggcac tggtactcaa ccgccacatt
    13141 gtgggattcc agcgccgcca gaagggaagg gtgttcatcc tggcacagca gttgtgtgcc
    13201 agtgatgcgc agggtaacgc gggccatcag agctggaagt tactcaaatc gtcggtgtcc
    13261 acttccgcgt caatctgccc gaccagataa gaactgactt ccacttcctg cggagcaacc
    13321 tgcacgttat cagacaccag ccaggtgttg atccacggga tcgggttgga gcgcgtctgg
    13381 aacggcagat ccaaaccgac tgcctgcatg cggatattgg tgatgtattc aacgtactgg
    13441 cagagaatat ctttattcag accaatcatc gaaccgtcac ggaacagata atccgcccag
    13501 tctttctctt gctgtgccgc ctggacaaac aggtcatagc actcctgctt acattcttcg
    13561 gcaatttccg ccatctccgg atcatccgcg ccgctgcgca gcagattcag catatgctga
    13621 gtaccggtca ggtgcagggc ttcatcgcgg gcaatcaggc gaataatctt ggcgttacct
    13681 tccatcagtt cgcgttctgc aaaggcaaag gaacaagcaa agctgacgta gaagcggatc
    13741 gcttccagcg cgttaacgct catcaggcag agatacagtt ttttcttcag ctcgcgcagg
    13801 ctaacggtta cggttttacc gttaacggtg tgggtgcctt cgcccagaag gtgccagtag
    13861 ctggtcattt cgatcagttc atcgtaatag ctggagatcc cttccgcacg tttctggatc
    13921 tgctcgttgg tgacgatatc gtcaaacaca acagacggat cgttaacgat attacgaatg
    13981 atatgagtat aggaacggga atgaatcgtt tctgagaacg cccaggtttc gacccaggtt
    14041 tccagttccg gaatagaaat aagcggcaat agcgccacgt tcgggctacg accctgaatg
    14101 gaatccagca gcgtctgata tttcaggttg ctgataaaga tgtgtttttc gtgctccggc
    14161 agcgcctggt aatctatacg gtcgcgggag acgtcaactt cttccggacg ccagaagaaa
    14221 gagagctgct tttcgatcag cttttcgaag atgtcatatt tttgctgatc gtagcgagcc
    14281 acgttgaccg gctgaccaaa gaacatcggt tctttgagct gatcattttt cgtctgtgaa
    14341 aaggtggtat atgccatgag tgtgtcctgt tgggagttta atgccggata aggcatttta
    14401 cgccgcatcc tgtgcctgat gcgacgctgg cgcgtcttat caggcctaca aatccgagcc
    14461 gtaggccgga taaggcgttc acgctgcatc cggcacccgg agcctgatgc gacgctggcg
    14521 cgtcttatca ggcctacaaa ccgagccgta ggccggataa ggcgtttacg ccgcatccgg
    14581 catctcaata tcagatctta catgcgccgc tttcgcagcc atcgtcctgg attgacggta
    14641 ccagatcgtc ctgcgcgtct tcagcgccgt cacgggtgtt ctgataatac agcgttttga
    14701 ccccgaattt gtaggcggtg agcaggtctt tcagcaactg ctgcatcggc acttttcctg
    14761 acgggaaacg tgacggatcg tagttggtgt tagcagagat cgactgatcg ataaatttct
    14821 gcatgatacc taccagttgc aggtagccat cgttacccgg catttcccac agcagctcat
    14881 aggcgtcgtg caggtgctcg tagtccggca ccacctggcg caaaataccg tctttcgacg
    14941 ctttgatgct gacgtaaccg cgcggcggtt caataccgtt agtggcgtta gagatctgcg
    15001 aagaggtctc ggacggcatc agggcagaaa gcgtggagtt acgcaggccg tgcgttttga
    15061 ttgattcacg caatgcttcc cagtcgtaat gcagcggctc attagcgatg gcatccagat
    15121 ctttcttata ggtatcgatc ggcaggatcc ctttcgcgta agtggtttcg ttaaaccacg
    15181 ggcacgcgcc ttgctcttta gccagctcat tagacgcttt cagcaggtaa tactgaatcg
    15241 cttcgaaggt tttatgcgtc aggttgttgg cgctgccgtc ggagtagcgt ttaccgtgct
    15301 tcgccagata gtaagcgaag ttaatcacac caatacccag cgtacgacgc cccatcgctc
    15361 cacgtttggc ggccgggatc gggtaatcct gataatccag tagcgcgtca agtgcacgaa
    15421 ccgccagaat cgccagctct tccagttcat ccaggctatt aattgcgccc aggttgaaag
    15481 cagacagcgt acacagcgcg atttcaccgt tctcgtcgtt gacgtcgttc agcggtttgg
    15541 tcggcagggc aatttctagg cacaggttag actggcgaac tggcgcgatg gtcggatcaa
    15601 acggactatg ggtattacag tggtcaacgt tctgaatata gatacgaccg gtagacgcac
    15661 gttcctgcat catcagcgag aacagctcaa cggctttcac acgctgcttg cggatgctgt
    15721 cgtctttctc atatttggta tacaggcgtt caaactcttc ctgatcggcg aagaacgcgt
    15781 cgtacagccc cggtacgtcg gacgggctga acagggtgat atcttcacct ttcagcaggc
    15841 gggtatacat cagtttgttg atttgtaccc cgtagtccat atgacgcacg cggttgcctt
    15901 caaccccacg gttgtttttc aacaccagca ggctttccac ttccagatgc cacatcgggt
    15961 agaacagcgt tgccgcaccg ccgcgcacac cgccctgaga gcaggatttc accgctgtct
    16021 ggaaatgttt gtagaacggg atacagccgg tatggaacgc ttcgccaccg cgaatcgggc
    16081 tgcccagcgc acgaatacgt ccggcgttga tgccgatccc ggcacgctgg gaaacatatt
    16141 tcacaatcgc gctggaggtg gcgttgatgg aatccaggct gtcaccgcac tcgatcagta
    16201 cgcaggagct gaactgacga gtcggggtac gcacgccgga catgattggc gtcggcagcg
    16261 aaattttaaa tgtggaaacc gcgtcataaa aacgcttcac gtattgcagg cgcgtttcac
    16321 gcgggtagtt cgagaacagg cacgcggcaa caagaatata aaggaactgg gcgctttcgt
    16381 agatttcgcc ggtcacgcgg ttctgtacta gatatttgcc ttccagctgc ttaacggcag
    16441 cataagagaa ggtcatatca cggtcgtgat cgataaacgt gtccatctgc ttgaactctt
    16501 cttccgtgta gtcttccagc agatgattat cgtatttgcc catctcgacc attttcacca
    16561 cgtggtcgta cagcgcaggc ggctcaaact ggccgtaggc ttttttacgc aggtggaaga
    16621 tcgccaggcg cgcggcgaga tactgataat ccggcgcatc acgggagatc aggtctgcgg
    16681 cagccttgat aatagtttcg tggatgtcag aggtcttgat accgtcataa aactgaatgt
    16741 gggagcgcag ctcgacctgg gaaatcgaaa cgttatgcag tccttctgcc gcccaatcca
    16801 gaacgcgatg gattttgtcg agattgatgc gctctgtgct accgtcgcgc tttgtcacca
    16861 gcagattctg attcatgtat gtcgtacctg tttttggaat tctttccgca atagtttcat
    16921 gcagactacc tgtagtaagg gaagctgctt agaacactat atataggggg tatgtttggg
    16981 attcactgca agatagtgtg aaaatgaccc tcttgcaagt gcataacttt gtggataact
    17041 caggaaggaa aaagtggctt tcgcgcacct taggtcagac aaggtgtccg ggaaagtcaa
    17101 tgggaagaga aaaatttgtt aaaaataact gttcggcgat ttcttgaacg gcagaatagg
    17161 ttggataaat tgatatagat ggtttattaa tctttttgct aatgattgca aaaattgatc
    17221 taagcacgag tcatcacata atctgatgta tcagttgtgg tcaatatata agcgaatgta
    17281 agcgattgtt gatgtcgaaa tggttgtttt ctattcggtt attgttaaat gcgaagccat
    17341 tttgttaatt taccttatat tataagaaaa ttattcatta ctttaattta gtttaaatat
    17401 aactggtatc ccttaaggtt attaacttga attatgacgc actgatatta ttcatcaaat
    17461 aataacaaaa tagccattgc atcgggttgg acaatttaca aaagaaagat aacccatatt
    17521 gtataatgcg tttaaatatg ccgtcttata taaaatgatg gtcagattaa tatgcggatt
    17581 atctttctac gcaaggagta tttatctcta ctcccgtcaa tgattgcatc tcttttctct
    17641 gctaacggtg tcgcggcggc cattgattta tgccagggat atgatatcaa agcgagttgt
    17701 cacgccagca ggcaaagcct ttcaggcatt ac
//
LOCUS       DAZSNG010000083        17641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZSNG010000083 DAZSNG010000000
VERSION     DAZSNG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17641
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.084"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..674)
                     /gene="chbR"
                     /locus_tag="ACHW4Z_002866"
                     /pseudo
     CDS             complement(1..674)
                     /gene="chbR"
                     /locus_tag="ACHW4Z_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(682..1032)
                     /gene="chbA"
                     /locus_tag="ACHW4Z_002867"
     CDS             complement(682..1032)
                     /gene="chbA"
                     /locus_tag="ACHW4Z_002867"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR8882428.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="ACHW4Z_002868"
                     /pseudo
     CDS             complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="ACHW4Z_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="ACHW4Z_002869"
     CDS             complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="ACHW4Z_002869"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR8882429.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="ACHW4Z_002870"
     CDS             complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="ACHW4Z_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR8882430.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3683..4510
                     /gene="nadE"
                     /locus_tag="ACHW4Z_002871"
     CDS             3683..4510
                     /gene="nadE"
                     /locus_tag="ACHW4Z_002871"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR8882431.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4740..5627
                     /gene="cho"
                     /locus_tag="ACHW4Z_002872"
     CDS             4740..5627
                     /gene="cho"
                     /locus_tag="ACHW4Z_002872"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR8882432.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5587..6162)
                     /gene="ves"
                     /locus_tag="ACHW4Z_002873"
     CDS             complement(5587..6162)
                     /gene="ves"
                     /locus_tag="ACHW4Z_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR8882433.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6365..6850)
                     /gene="spy"
                     /locus_tag="ACHW4Z_002874"
     CDS             complement(6365..6850)
                     /gene="spy"
                     /locus_tag="ACHW4Z_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR8882434.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7180..8148)
                     /gene="astE"
                     /locus_tag="ACHW4Z_002875"
     CDS             complement(7180..8148)
                     /gene="astE"
                     /locus_tag="ACHW4Z_002875"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR8882435.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8141..9474)
                     /gene="astB"
                     /locus_tag="ACHW4Z_002876"
                     /pseudo
     CDS             complement(8141..9474)
                     /gene="astB"
                     /locus_tag="ACHW4Z_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9471..10949)
                     /gene="astD"
                     /locus_tag="ACHW4Z_002877"
     CDS             complement(9471..10949)
                     /gene="astD"
                     /locus_tag="ACHW4Z_002877"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR8882436.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10946..11980)
                     /gene="astA"
                     /locus_tag="ACHW4Z_002878"
     CDS             complement(10946..11980)
                     /gene="astA"
                     /locus_tag="ACHW4Z_002878"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR8882437.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11977..13197)
                     /gene="astC"
                     /locus_tag="ACHW4Z_002879"
                     /pseudo
     CDS             complement(11977..13197)
                     /gene="astC"
                     /locus_tag="ACHW4Z_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13634..14440
                     /gene="xthA"
                     /locus_tag="ACHW4Z_002880"
     CDS             13634..14440
                     /gene="xthA"
                     /locus_tag="ACHW4Z_002880"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR8882438.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14607..15320
                     /locus_tag="ACHW4Z_002881"
     CDS             14607..15320
                     /locus_tag="ACHW4Z_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8882439.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            15325..16002
                     /gene="ydjY"
                     /locus_tag="ACHW4Z_002882"
     CDS             15325..16002
                     /gene="ydjY"
                     /locus_tag="ACHW4Z_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR8882440.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16017..16724
                     /locus_tag="ACHW4Z_002883"
     CDS             16017..16724
                     /locus_tag="ACHW4Z_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8882441.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16724..17272
                     /locus_tag="ACHW4Z_002884"
     CDS             16724..17272
                     /locus_tag="ACHW4Z_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR8882442.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17282..17608
                     /locus_tag="ACHW4Z_002885"
                     /pseudo
     CDS             17282..17608
                     /locus_tag="ACHW4Z_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4361 a         4688 c         4307 g         4285 t
ORIGIN      
        1 atctcattaa taatctgcat tggtgttttg ccataatatc gttgagtcgc tcgcgtcaaa
       61 tattcctgtg atttggctga caacgtcacc atattctcca gcgccgattc actaaactgc
      121 tctttatcat gcatcttttc taccgtactt ttcagccact acggtacatc atcaatcacc
      181 tgttcttcgc ggtaatgacg taaacggtta atgacataaa aagtaaccat ctcaacaaac
      241 tcttccagcc ctgtttcgcg gaaatttaat gaagaaatca ctgtttccac ataggtgaga
      301 aaagcgttat tggtccggta tacctgcgaa gcaacaaagc aataaggcaa caacggcagg
      361 taatgctgct caaaaaagcg tttactgatc ccaacgttca atatgcgcgt ggcaccaaac
      421 tcataaaaac tttgatggtg cgaacctaac ggaataaaaa caaaatcgcc ccgttccagt
      481 aacacgcgct taccgttaat ctcctggaaa taacgcccgg ttaataccag agtaaattca
      541 taatagtcgt gctgatgcag tccgctgata ctctcagttt tgttatagat aaacacatgg
      601 aagtttttgc cattaaacaa ctgctgttct cgggcagtgg caatttccgg cgcgttaatc
      661 actggctgca tcatcgactc cttatgcctt cagtttttca tgaagctcaa ttaattcagt
      721 aatcagttca cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga ccagcaccag
      781 actcactttc atcttacctt cgcccgcatc gccttcaatc agtttcgtct gtaccagatg
      841 cgcttcattc aatgccatcc gtgactgatc catcatggct ttcgctgcgg caaaatcgcc
      901 ctgcttcgcc tgtttcaatg cggcatacgc caggctacgc gcttgtccag agttgatgat
      961 aagccccatc accacttctt ccagctcttc agcttctgtt tgcgtatcag gaatattatc
     1021 gagatccatc atacatcgtt cctctctctt cttaccggca agattacccg taccggcatc
     1081 gattaaaatt tcaggggtta gcgatatctt cttcgctctc ttctttatca atcgcatttt
     1141 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgatg ccaaggttga
     1201 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     1261 ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg
     1321 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     1381 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
     1441 gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga
     1501 tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg
     1561 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     1621 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     1681 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     1741 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     1801 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     1861 agccgggaat taatgccgag aaagaacgcg atactgaagc aggtacgctg tcgggcagtt
     1921 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     1981 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
     2041 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca gccgccagcg
     2101 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     2161 ttattcctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
     2221 caatggttga ggcgtcgagg cgaataccta aggaataaaa aacgacccct ccccaaagct
     2281 tagaaaaacg ttgttaatta atacaaacat ggcccccgca agggttaacg gcattaagcg
     2341 aataaagcca tttttgattg cattaacgtg tggctgcttt cctattttaa ctgcaaaagg
     2401 gaggagtacc ttttcaagcg atgcaataac attactcata gaaaaatacc cttaaaaacc
     2461 gcaatttaaa tattacggta ttgatttatg aaataactct ttgacgggaa aatttaaaat
     2521 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
     2581 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
     2641 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
     2701 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
     2761 ttctgcctgt gcgcgcattt ttgataccag taaagagata gacatgcccg cagaacaaaa
     2821 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
     2881 cactccgcaa gccttgacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
     2941 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
     3001 taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg
     3061 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
     3121 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
     3181 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
     3241 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
     3301 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
     3361 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagctgtac
     3421 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
     3481 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
     3541 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     3601 attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag gaagttttgt
     3661 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     3721 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     3781 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     3841 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg aaactcgcaa
     3901 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg acgaacaaga
     3961 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     4021 cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga gcgattttgt
     4081 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     4141 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     4201 atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac gccagggtaa
     4261 acagttactg acggcattag gttgcccgga acacctttat aagaaagccc caactgccga
     4321 tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     4381 tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa caatagagaa
     4441 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg atgatttctg
     4501 gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     4561 atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac attgcaggag
     4621 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     4681 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     4741 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     4801 ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     4861 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     4921 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     4981 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     5041 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     5101 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     5161 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     5221 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     5281 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     5341 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt tggccttggc
     5401 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     5461 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     5521 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     5581 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     5641 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacacgcc
     5701 ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca caaacaccac
     5761 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     5821 gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg tcagtttcgc
     5881 ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     5941 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     6001 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     6061 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg
     6121 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     6181 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     6241 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     6301 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     6361 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     6421 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     6481 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     6541 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     6601 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgtggatct
     6661 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     6721 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     6781 cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     6841 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     6901 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     6961 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     7021 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaactatacc
     7081 ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat ttataagaaa
     7141 gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt tttcgagcat
     7201 taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat actctacatc
     7261 atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg
     7321 cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt ggcgagtaat
     7381 ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac tctcaccaga
     7441 tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa
     7501 gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc tgacgctgaa
     7561 atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc ccgccgcacc
     7621 cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg
     7681 cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca cagattcttt
     7741 gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac aggtttctcc
     7801 gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc tatggcaata
     7861 acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc gccaacgtaa
     7921 cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca caggtgccgt
     7981 ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg ttaatggcgt
     8041 cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt
     8101 gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg ccccctccct
     8161 cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca tccagtgctt
     8221 cccgcccttc gcgcagcaat tgcgggtcgg ccaaatcggc agcagtaaga cgatcgcggt
     8281 agtaacgatc cacccagtca ttgagcgcat taaacaaggt atcgttcatc atcaccgccg
     8341 gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg cacgccgggc
     8401 cgccgccatt cgccatgctt tcacgtaagt caaagacttt tagttcgcta atcgggttgt
     8461 cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga cactcctgag
     8521 gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta aacagatagg
     8581 tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc ataaaaccgt
     8641 tcacccgcgt gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac
     8701 ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg ctctggtcga taacgtccgg
     8761 gttttgctgg gcgaaaatca cctgttgggg attcacctga ttaagccttg ccaccgcctc
     8821 gctggcttcg cgagtctgtc gcgccggata acgggaaggc cgggtatcat tgccttcttc
     8881 tcgcccgtag acaaaaagta gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg
     8941 gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc aacgccgaat ggacgctaaa
     9001 tttctcttcg tccttaaaaa tcgcttttaa cagcgattca gtgacgggcg cttccagcga
     9061 acggtgaaat ttattgttca ggttggcaac ggtgagatgc actttgccat ccagcgtatc
     9121 ggcagatggc gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct
     9181 ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact
     9241 gaatcccaac tgacgcagca ccggaataaa cggacgctcg tgcggcggaa tcacggcctg
     9301 ggggaatccc gcatcggcaa gggctttcat tttcagtaag ccctgttttg ccgccagtcg
     9361 cgggttagac acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggcccgc
     9421 gtaatgatgc gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc
     9481 acctcatcgg aaaaatccag cccggggtta agcgtggcgg gcaatgttaa cgagtccgac
     9541 tccaggctcg ccataggcca tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg
     9601 ttaccggaag caccaatgcc gccgaatggg gcggtactgg cagcaccagt aagcggtttg
     9661 ttccagttaa caatccccgc ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg
     9721 ggggaaacca gaccgcaaaa gagtccgaag cgagtgttgt tcgccatccg aatcgcctca
     9781 tcaaaagtat cataacgcca gacgcgcaaa aacggcccga acacctcttc atccggcaca
     9841 ccgccaacgc ccgtcatttc aatgatccct ggtgtcagca acgatgtccc tgcttgtaat
     9901 aagcgcggcg caagcagggt tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc
     9961 acctgctgtg cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc
    10021 cagttgcccg gcgttaatcg ctggctgacg gcaaccagac tagcaagaaa cgcatcgccc
    10081 tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca
    10141 aacgccgact gaatggtcag atggacagcc gcgtcgatat ccgccacctc atcgataatt
    10201 agcggattat taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg
    10261 cgatgcagct ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc
    10321 agcgcactca gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc
    10381 ggcaagccag cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg
    10441 ctgggtttaa agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc
    10501 aaatgaccag ggaaattata cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc
    10561 aggctcgccg caccgtccgg catttcacta cgctgctcgc cggtacgaac gtgatacgcc
    10621 ttaattgata tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc
    10681 ggcttacccg tttctctggc aataatcgcg gttaattcgg ctttattgct ttccagcaat
    10741 ccggcaaagc gttcgacaac gacctgacgt tcagccaatg agagccgcgc ccagcgcgga
    10801 aacgccgcac gggctgcccg acaagcctgt tcgacctgag cggcatcggc atcattgcct
    10861 tgccataaca cctcgcccga taccggatta cgcttcacac gcgatgcgcc ctggcccgtt
    10921 atccagtcac cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca
    10981 gacgaacgcg atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca
    11041 aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga taattttcat
    11101 tggcgaccag gcaggccgga aaatcgccct gcgcaggctg cccttctgcc acttccacca
    11161 gccgactttt acggatggcg cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt
    11221 cgaagatgtc gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag
    11281 gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg
    11341 tatagatcgg atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga
    11401 gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg
    11461 gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc aaccactttg tcattaaact
    11521 tgtcgcgaaa agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct
    11581 ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt
    11641 tactaagaaa cagcgtcggc aatgcgttat agacattcag ctcttttgag gcgtgaacca
    11701 acgtgccgac gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatagcgc
    11761 aaatccccgc cacggtgcct gtctcgctat cttccagcac gaacacatag ccctgctcgc
    11821 ttttgggcag ttcaccttgc caggttttga ttgccctttc gatacgcgcc gaaagcgtgg
    11881 cttcattggc gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg
    11941 ccgagacatc tgagcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa
    12001 gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt
    12061 gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt
    12121 cgccgcttcc agagagagct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc
    12181 aatcaacaaa cctaagccgc gaacttcact gaacaaacca tagcggtgat taatagtatt
    12241 aagacgctca acaaaccagt cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt
    12301 gatgagctcc agcactttgc ctgctaccgc cgaggccagc ggattaccgc cataggtggt
    12361 gccatgagtg ccaacggtca tcacgcgggc gcactcttcg gttgccaaca acgcaccgac
    12421 cgggaaaccg ccgcccagcg ctttggcggt ggttaacaga tcgggcgtca cgccgtagtg
    12481 catataggca tacagctcac cagtgcgccc gacgccggtt tgtacctcat caaaaatcag
    12541 caaggcattg tggcggtcac acaattcacg cagaccttgt aaaaacgcgt tgctggctgg
    12601 caccacaccg ccttcgccct agatgggctc gacaatcacc gcacaggtgg cgtcgtcaat
    12661 cagcgcgctg gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg
    12721 cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc
    12781 atgaaacgca tttttgaacg ccacgatgcc actcttatgg ctgccgtagc ggtcgtgagc
    12841 gaatttacgc gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa
    12901 gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt
    12961 ggtgtaaccg ttgccggtat gccagaactt actcgcctgt tcgttcagcg cttcacgcag
    13021 ttccggatgc gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc
    13081 tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt accggtataa agggtgccgg
    13141 agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc
    13201 gacctctaca gtaaataatt cgttatttgt atgttaacaa taagtaatgt ttgcgctgta
    13261 aatgtagatt gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa
    13321 ctggaaatca aggagttata accaaaccat atgcatttaa agtgcatata aagtgaatac
    13381 gtttgcgatg tgggtgaata aaaaacgcaa tgttatgcag aagtaaaata taattctaga
    13441 attgtgatca ttgacgaaat ttactggaaa ttactgcgcc attctgacgc agcgcgcacc
    13501 aaaagcgggc attttttgcg ccatcgttga catcattaac aaccatcgat caaatcactt
    13561 aacaacaggc ggtaagcaac gcgaaattct gctaccatcc acgcactctt tatctgaata
    13621 aatggcagcg actatgaaat ttgtctcttt taatatcaac ggcctgcgcg ccagacctca
    13681 ccagcttgaa gccatcgtcg aaaagcacca accggatgtg attggcctgc aggagacaaa
    13741 agttcatgac gatatgtttc cgctcgaaga ggtggcgaag ctcggctaca acgtgtttta
    13801 tcacgggcag aaaggccatt atggcgtggc gctgctgacc aaagagacgc cgattgccgt
    13861 gcgtcgcggc tttcccggtg acgacgaaga ggcgcagcgg cggattatta tggcggaaat
    13921 cccctcactg ctgggtaatg tcaccgtgat caacggttac ttcccgcagg gtgaaagccg
    13981 cgaccatccg ataaaattcc cggcaaaagc gcagttttat cagaatctgc aaaactacct
    14041 ggaaaccgaa ctcaaacgtg ataatccggt actgattatg ggcgatatga atatcagccc
    14101 tacagatctg gatatcggca ttggcgaaga aaaccgtaag cgctggctgc gtaccggtaa
    14161 atgctctttc ctgccggaag agcgcgaatg gatggacagg ctgatgagct ggggggtggt
    14221 cgataccttc cgccatgcta atccgcaaac agcagatcgt ttctcatggt ttgattaccg
    14281 ctcaaaaggt tttgacgata accgtggtct gcgcatcgac ctgctgctcg ccagccaacc
    14341 gctggcagaa tgttgcgtag aaaccggcat cgactatgaa attcgcagca tggaaaaacc
    14401 gtccgatcac gcccccgtct gggcgacctt ccgccgctaa tttagcagct ctcctggctc
    14461 aaactgggtc aggagaatta accttgagaa aaatcaacaa actgtcagta atgatttgtt
    14521 gcctgccgtc ctttgttata ccgtcactgc gtttttagtt gtctgaccac ttctctatta
    14581 tcaagtttga tataggaaac tccacgatga acgctgagcg taactttctg tttgcctgtc
    14641 ttatttttgc gctggtcatt tacgctatcc acgctttcgg tttattcgat ctgctcaccg
    14701 atttacccca cttacagaca ctcattcgcc agagcggatt gttcggttat agcctctata
    14761 ttctgttatt catcaatgcc cccctctttc tgttaccagg aagcatactg gtgatcgctg
    14821 gaggaatagt ttttggcccg ctcttaggga cacaactctc attaattgcc gcaacgctgg
    14881 cctcctcgtg ctcattcctg ctggcgcgct ggctgggacg tgatttactg ctgaaatacg
    14941 tgggccatag ccataccttc caggccattg aaaaaggcat tgcgcgtaac ggtattgatt
    15001 ttcttattct gacccgctta atcccgttgt ttccttacaa tattcaaaat tacgcttacg
    15061 gattcaccac catcgccttc tggccttata cccttatttc ggcactcacg accctgcccg
    15121 gtattgttat ttataccgtg atggcaagcg atctcgccaa tgaaggcatt acgctgcgct
    15181 ttattttaca actctgtctg gcgggcctgg cgctgtttat tctcatccag ctcgcaaaac
    15241 tactctacgc ccgacacaaa catgtggatc tgtctgcttc gcgccgcagc ccacttactc
    15301 acccaaaaaa tgaaggatag aacgatgtcg caacattatt cagtgtcatg gaaaaaagga
    15361 ctggctgcac tctgcttact ggctgttgcc gggcttagcg gctgcgatca aaaagagaat
    15421 gcggcggcaa aagtggaata cgacggactt tcgaacagcc aaccgctgcg cgtcgatgcc
    15481 aataaccata cggtaaccat gctggtgcaa attaatggtc gtttcctcac cgacgacact
    15541 cgtcacggta ttgtgtttaa agatggctcc aacggacata aatcgctgtt tatggcttat
    15601 gcgcccccga aagcatttta tgaagccctg aaagaggcag gtggtacgcc gggcgaaaac
    15661 atgacgatgg ataataaaga aacgactcat gtcacaggca gcaaactgga tatttcggtc
    15721 aactggcaag gggcggcaaa agcgtattcc ttcgatgaag tgattgttga cagtaatggc
    15781 aagaaactgg acatgcgctt tggcggtaat ttaacggcag cagaagagaa gaaaacgggt
    15841 tgcctggtgt gtctggatag ctgcccggtc ggcatcgtca gcaatgcaac atacacttat
    15901 ggtgcggtgg aaaaacacgg cgaagtgaaa ttcaaaggca atgcctcagt tctcccggcg
    15961 gataacacgc tggcaacggt tacctttaaa atcaccgaat aaagccaaga taaaggatga
    16021 tgaagataca atcgcgtaaa atctggtact accgtataac cctcatcatc ctgttgttcg
    16081 ctatgctgct cgcatgggcg ctgcttcccg gcgtccatga gtttatcaat cgcagcgttg
    16141 cggcgtttgc cgccgtggac caacagggta tagaacgctt tattcagtct tacggcgcac
    16201 tggcggcagt tgtctcgttc ttgttgatga ttttacaggc cattgctgca ccgctacctg
    16261 cgtttttgat cacctttgcc aatgcgtcgc tgtttggcgc gttctggggc ggcttgctgt
    16321 cgtggaccag ttcgatggcc ggcgcggcgc tgtgcttttt tatcgccaga gtgatgggcc
    16381 gcgaagtggt ggaaaaatta accggcaaaa ccgtgcttga cagtatggac ggctttttca
    16441 ctcgctacgg caaacacacc atcctggtct gtcggttatt gccttttgtc cctttcgatc
    16501 caatcagcta tgctgccggt ctgacttcaa tacgttttcg ctcgtttttt atcgccaccg
    16561 ggcttggtca attaccggcg actattgttt attcctgggc aggcagcatg ttaacaggcg
    16621 gtactttctg gtttgtcacc gaactgttta ttctgtttgc cctgaccgtg gtgattttta
    16681 tggcgaagaa aatatggctt gagcgccaga agaggaatga ctgatgggtt taccgccgct
    16741 tagcaaaatt cctttcattt tacgtccgca ggcgtggctg catcgtcgcc attacggcga
    16801 ggtgctaagc ccgattcgct ggtgggggcg gatcccgttt atcttttatt tggtgtcaat
    16861 gtttgttggg tggctggagc gcaaacgctc accgctcgat ccggtagtgc gatcgcttgt
    16921 cagcgcgcgc attgcgcaaa tgtgcctgtg tgagttttgc gtggatatca ccagtatgaa
    16981 agtcgccgag cgtaccggca gcaccgataa actgctggca gtggctgact ggcggcaaag
    17041 cccgctcttt agcgatgaag aacggctggc gctggagtac gccgaagccg caagcgtcac
    17101 accgccaaca gtcgatgatg ccctgcgtac ccgactggct gcgcattttg acgctcaggc
    17161 gctcaccgaa ctgacggcat tgatcggcct gcaaaacctg tcggctcgtt ttaattctgc
    17221 catggacatt cccgctcagg ggctgtgccg tattccggaa aaacgttctt aaggagggaa
    17281 gatgcgccat tgtgggtggt tgctgggatt gttatcgctg ttttctttgg caacacatgc
    17341 cagtgactgg caggaaatta aaaatgaggc caaagggcaa accgtctggt ttaacgcctg
    17401 gggcggcgat accgcaatta accgctatct cgactgggtt agcggcgaga tgaaaaccca
    17461 ttacgctata aacctgaaga ttgttcgtct cgcggatgcc gcagacgcgg tgaagcgcat
    17521 tcagaccgaa gctgctgccg gtcgtaaaac gggcggctcg gtggatctgc tctgggtgaa
    17581 cggcgaaaac ttccgcacct taaaagagta atgccagtca gttaagcaac tgactggcat
    17641 t
//
LOCUS       DAZSNG010000084        17555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZSNG010000084 DAZSNG010000000
VERSION     DAZSNG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17555
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.085"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..266
                     /locus_tag="ACHW4Z_002886"
     CDS             <1..266
                     /locus_tag="ACHW4Z_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8882443.1"
                     /translation="ATLERLLSLLSAFEVVVWMTDGWPLYESRLKEKLHVISKRYTQR
                     IERHNLNLRQHLARLGRMSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            610..732
                     /locus_tag="ACHW4Z_002887"
     CDS             610..732
                     /locus_tag="ACHW4Z_002887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882444.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            729..1295
                     /gene="mntP"
                     /locus_tag="ACHW4Z_002888"
     CDS             729..1295
                     /gene="mntP"
                     /locus_tag="ACHW4Z_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR8882445.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1292..2101)
                     /gene="rlmA"
                     /locus_tag="ACHW4Z_002889"
     CDS             complement(1292..2101)
                     /gene="rlmA"
                     /locus_tag="ACHW4Z_002889"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR8882446.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(2267..2476)
                     /gene="cspE"
                     /locus_tag="ACHW4Z_002890"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(2267..2476)
                     /gene="cspE"
                     /locus_tag="ACHW4Z_002890"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8882447.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2489..2632)
                     /locus_tag="ACHW4Z_002891"
     CDS             complement(2489..2632)
                     /locus_tag="ACHW4Z_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR8882448.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3303..3590)
                     /locus_tag="ACHW4Z_002892"
     CDS             complement(3303..3590)
                     /locus_tag="ACHW4Z_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR8882449.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3665..3808)
                     /gene="mgrB"
                     /locus_tag="ACHW4Z_002893"
     CDS             complement(3665..3808)
                     /gene="mgrB"
                     /locus_tag="ACHW4Z_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR8882450.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3967..4206
                     /locus_tag="ACHW4Z_002894"
     CDS             3967..4206
                     /locus_tag="ACHW4Z_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR8882451.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4349..5140)
                     /gene="kdgR"
                     /locus_tag="ACHW4Z_002895"
     CDS             complement(4349..5140)
                     /gene="kdgR"
                     /locus_tag="ACHW4Z_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR8882452.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5317..6696
                     /locus_tag="ACHW4Z_002896"
     CDS             5317..6696
                     /locus_tag="ACHW4Z_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882453.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSASAP
                     RERSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGL
                     RITQPRARA"
     gene            complement(6742..7623)
                     /gene="htpX"
                     /locus_tag="ACHW4Z_002897"
     CDS             complement(6742..7623)
                     /gene="htpX"
                     /locus_tag="ACHW4Z_002897"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR8882454.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7815..9863)
                     /gene="prc"
                     /locus_tag="ACHW4Z_002898"
     CDS             complement(7815..9863)
                     /gene="prc"
                     /locus_tag="ACHW4Z_002898"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR8882455.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9883..10581)
                     /gene="proQ"
                     /locus_tag="ACHW4Z_002899"
     CDS             complement(9883..10581)
                     /gene="proQ"
                     /locus_tag="ACHW4Z_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR8882456.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10678..11175)
                     /gene="msrC"
                     /locus_tag="ACHW4Z_002900"
     CDS             complement(10678..11175)
                     /gene="msrC"
                     /locus_tag="ACHW4Z_002900"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR8882457.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11305..12588
                     /gene="yebS"
                     /locus_tag="ACHW4Z_002901"
     CDS             11305..12588
                     /gene="yebS"
                     /locus_tag="ACHW4Z_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR8882458.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12557..15177
                     /gene="yebT"
                     /locus_tag="ACHW4Z_002902"
                     /pseudo
     CDS             12557..15177
                     /gene="yebT"
                     /locus_tag="ACHW4Z_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            15257..16696
                     /gene="rsmF"
                     /locus_tag="ACHW4Z_002903"
                     /pseudo
     CDS             15257..16696
                     /gene="rsmF"
                     /locus_tag="ACHW4Z_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16814..17050
                     /locus_tag="ACHW4Z_002904"
     CDS             16814..17050
                     /locus_tag="ACHW4Z_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR8882459.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            17155..17346
                     /locus_tag="ACHW4Z_002905"
     CDS             17155..17346
                     /locus_tag="ACHW4Z_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR8882460.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17371..>17555)
                     /locus_tag="ACHW4Z_002906"
     CDS             complement(17371..>17555)
                     /locus_tag="ACHW4Z_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR8882461.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4260 a         4424 c         4239 g         4632 t
ORIGIN      
        1 tggccacact ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga
       61 cggatggctg gccgctgtat gaatcacgcc tgaaggaaaa gctgcacgtt atcagcaagc
      121 gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctgg
      181 gacggatgtc actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt
      241 atctgaacat aaaacactat cagtaagttg gagtcattac cctcgtatca actcaataac
      301 ttatgaattt agccaaagct atgtttagtg tatttttaat aatcagacat agcttaggct
      361 atattatctc ttcccttatt tgttatttat tttaacgttt cattgatata taaacataaa
      421 tgaaaatcgt tatcaataaa gcaatgaaat aatatattcc aacagttgtt ttatattctc
      481 aaaatatgtt aaggttgcgc cctcatttgg ggagtagccg atttccagat tccggaaatg
      541 tacgtgtcaa catactcgtt gcaaaacgtg gcacgtacgg actgaagact ttcagtcagg
      601 cgagaccata tgcacatcaa tcgctatgcc tgcatgaacg cgaaaaccca tcgcggcttg
      661 ttgttcatgt atgcatgttt gctgggggcg atgatgtgtt ttatggatac cccggtcagg
      721 acgttgtcat gaatatcact gctactgttc ttcttgcgtt tggtatgtcg atggatgcat
      781 ttgctgcatc aatcggtaaa ggtgcccccc tccataaacc gaaattttct gaagcattgc
      841 gaaccggcct tatttttggt gccgtcgaaa ccctgacgcc gctgatcggc tggggaatgg
      901 gcatgttagc cagccggttt gtccttgaat ggaatcactg gattgcgttt gtgctgctga
      961 tattcctcgg cgggcgaatg attattgagg gttttcgtgg cgcagatgat gaagatgaag
     1021 agccgcgccg tcggcacggt ttctggctac tggtaaccac cgcgattgcc accagcctgg
     1081 atgccatggc tgtgggtgtt ggtcttgctt tccttcaggt caacattatc gcgaccgcat
     1141 tggccattgg ttgtgcaacc ttgattatgt cgacattagg gatgatggtt ggtcgcttta
     1201 tcggctcaat tattgggaaa aaagcggaaa ttctcggcgg gctggtgctg atcggcatcg
     1261 gcgtccagat cctctggacg cacttccacg gttaataaga acgctgccag aggtgaatat
     1321 taaagtccgt ctggcagtcg aacacttctt ttgctgccag tgtttgccag acttctggct
     1381 tcgcacgcca ggcaaacggc gtcatctgca ataatgcgac ggcttcatca ccgcgaagac
     1441 gcatcggata acacaactcc gcactctgct gtaatgtaaa accttccagt tgttctgcat
     1501 gaggtgcatg aagatgtact tcattgtaaa tcagcccctt cagctccatc aaatgtcgcg
     1561 gtcccggcgt ggcagtaatg acccagccgc cgggcttcac tactcgtgct aattcttctg
     1621 ctttacacgg cgcgtaaata cgtattatgg cgtccatact ggtatcggaa aacggcaaac
     1681 ggtggctgga agcgacacaa agagtgacct gcggatagcg tttcgccgcc gcttttatcg
     1741 ctaccttcga gacatccagg ccaaacgtgg tgatttcggg caacgcatcg gcaaatgcgt
     1801 gtgtgtaata cccttcacca cagccaatat ccagcaccgc cgtggccttt tcatcaagcc
     1861 gttcccgcag ttgggcgaca attgcatcac gcagcggctg ataatgtccg gcatctaaga
     1921 atgcgcggcg tgcttgcatc atttccgcgc tgtcgcccgg atcacgagac cgtttatgct
     1981 gaacgggcag cagattgaca tacccttctt tcgccatatc aaactgatgt cgttggggac
     2041 agatatagct gtttttttca cgcgaaagag gctgatggca aagtggacag gaaaacgaca
     2101 tgacaactcc ggcaggatac taaaggccgc aagtgtaacg cgaatcacgc ggcgggcaaa
     2161 tatccgaacg ccaggagcga agataaagca tagtaaaaag cctcgcattc gacgaggctt
     2221 tatagcgaga ttgaagcgta ttcacacttc agatcagtgg attcgatcag atagctgtta
     2281 cgttaacagc tgccggacct ttctggccgt cctgaatttc gaactcaacg ttctgacctt
     2341 cagccagagt tttgaagcca ttaccctgga tagcggagaa gtgtacgaac acatctttgc
     2401 tgccatcagc cggagtaatg aagccaaaac ctttagactc gttgaaccac ttaacctgac
     2461 ctttaatctt tgccatttga aaaattcctt agattgtttt cttcgcccgc aggcataaca
     2521 tagataaaac tgacacatta ctgcatgagg caccaatata aggctcggca gagaagcggt
     2581 attcaacgtc aacgtgttta ctcaggactt ctttactgaa aatgccacac ataaacagaa
     2641 ctgtacctcg tttaacccga aatcttttat cacatacaac gttaattatg gcaagccatt
     2701 tttaaacata tctcgatcag acgcacaaat ctcgatacgt cttccacttt ttttgcacac
     2761 ttatgcaacg gaatacgcgc cgatatatca ttgtgcttaa ccttgccagt tcaggcagat
     2821 acttaaactg gcgtagtttc taacatagtt caataagttc agttgttcca ggccagaggg
     2881 agaagttatt acatagtgcg tgcaatatca cattttctgc tatgcaatga ataaaaagtt
     2941 atatcacttt ttctcataaa acagtcagtt aacggctatc aattacccta aagagaagtc
     3001 aatccccaaa gggattgtaa atttaaaata agaaaaattg atgagtgagc aaaaaaaatc
     3061 aagagagaaa cgtttcgcta ataaataata ccacgcttaa tcacaaatct gatgtaaaca
     3121 tcctaataaa ttaatgagga aaatatcttt ttctgatatc ccgttacgca aataaataat
     3181 atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagttgcac catgacagtg
     3241 cgtataatca ggatgaattg agtaaccctt caacgacggg gttactcaag attgaggaag
     3301 gattatcgtt cggcgaccag gcgaataata tcttcgtcat caaccgattt ttgactctca
     3361 atggtctttt tatctgcttt ctctggttcg tttgcttcgc acaaacgacg taacagtgca
     3421 ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc gttagtccgt
     3481 gaactggcac gattgataaa aaaccacaag atgagcccga taagaagaac caccaccgat
     3541 acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat ttcaccacct
     3601 ctgagtagag gcgctattct accactgctg gagaggaaga aaatctagtg ctgaaaaaat
     3661 gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat tgtacatcct
     3721 gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac accacgacaa
     3781 ccagaacgac ccatcgaaac tttttcactc caatctccgc ttcacctacc ttatgtcatt
     3841 aaactagcat gcgttagtta aacagcgcct aactatgtcg atattcgtgc tttttcggaa
     3901 tgagtagctt gcttcagcgc atattgccgc tacgattaag cgaacataaa aagagaagag
     3961 attgtaatgc gattcatcat tcgcacagtt atgctgatag cactggtatg gataggatta
     4021 ttactcagcg gatatggcgt tttgattgga agcaaagaga atgccgcagg attaggtttg
     4081 caatgtacct atctgactgc cagaggcacc agcacggtac aatacttgca cactaaaagt
     4141 ggtttcttgg agataacaga ttgcccactg ttacgtaaaa gcaatatcgt ggtcgataat
     4201 ggttaagggt aataaataac cagattctca ctcatgagta aaatattctt atagcaagcg
     4261 cgtttaatat aacgcacctt caactttatt tttctcatgc taaataaaga acctgtctgt
     4321 ttgatattcc gacaggttct tactctcatc agaacggata gtcgtgataa cccatttggg
     4381 cagaaatttt gcgcgctgcg gtatgcaaca ttgcgacata ttcctgtaaa cgctcttcag
     4441 agaaacgcaa cgtcgggaag gagatgctca aaccggcaat gaccacgcca aagcgatcaa
     4501 ataccggtac cgcaatgcat tgcagccctt cttcctgctc ttcgttatct tctccatacc
     4561 cctgctcgcg cacctggtcc agaacgggta ataacgcttc tgtactggtg atggtccgct
     4621 cggtactgcg tttatactcc acgccctcaa gaatttgctt cacttcatcg cgatcgcgcc
     4681 atgccagcag taccttacca atcgcggtgc tgtacagcgg attacgacgc ccaatccgtg
     4741 aatacatgcg caaattgtac atagagtcaa ttttgtgaat gtaaacaata ctgtcttcgt
     4801 ccagtgcgcc gaggtggata gtttctttgg tcaggcggga gatctcacgc atctggatat
     4861 ctgcgctacg aattaaatcg acgttttgta acgcgcgagc gcccagttca aacaatttca
     4921 gggtcaggga atatttctcc gactcccctt cctgcgccac ataacctaag gttttcatgg
     4981 tctgtaaaaa gcgataaacg gtgctttttg acatcatgac gcgctgcgac agctcggtta
     5041 tccctatttc gcgctcttca cccagcgcct gcaaaatgcc aaaaactttt agcacggaag
     5101 atacagaatc aggctgttta tccagatctg cgttagccat ttatcacctc attgcgagtg
     5161 ttttataaaa atcagaactg ttttttatta taatttcgca ccagggtggt cgcaatccat
     5221 cttttgccgg ttagttacaa ttctgcgaca tccaccgtga atatcagtgc tagaatcata
     5281 cccctgttga ttattcacca aagatataaa attcctatgc caaaagttca ggccgacggc
     5341 ctgccattgc cccagcgata cggtgcgata ttaaccattg tgattggtat ttcgatggct
     5401 gtccttgacg gcgcaatcgc caacgtcgcc ctgccaacaa ttgccacgga ccttcatgcc
     5461 acaccagcca gttccatctg ggtagtgaac gcctatcaaa tcgccattgt catctccctg
     5521 ctctcgtttt cgtttctggg cgatatgttt ggctatcgac gtatttataa atgcggtctg
     5581 gtcgtttttc tgttgtcttc actgttctgc gccctttctg attcgctgca aatgctcacc
     5641 cttgcgcgtg tcatacaagg tttcggcggt gcagcgttga tgagcgttaa taccgcactt
     5701 atccgcctga tctatccaca acgttttctg gggagaggga tgggcataaa ctcgtttatt
     5761 gttgccgtct cttctgctgc cgggccgaca attgctgcag caatcctctc catcgcatcc
     5821 tggaaatggt tatttttaat caacgtaccg ttaggtatta tcgccctgct tctggcgata
     5881 cgttttctgc cacccaatgg ttctcgcgcc agtaaacccc gtttcgacct gcccagcgcc
     5941 gtgatgaacg cgttaacctt cggcctgctt atcactgcgt tgagtggttt cgctcagggg
     6001 caatcgctga cgttaattgc tgcggaactg gtggtaatgg ttgttgttgg tattttcttt
     6061 attcgccgcc agctttctct tcccgtaccg ctgctaccgg tggatttact gcgtatcccg
     6121 ctgttttcac tttctatttg cacatctgtt tgctctttct gcgcacaaat gctggcaatg
     6181 gtttccctgc ccttttacct gcaaaccgtg ctcgggcgta gtgaagtcga aacaggttta
     6241 cttctgacac cgtggccgtt agcaacgatg gtgatggctc ctctggcagg ctatttgatt
     6301 gaacgcgtac atgcaggatt gctgggtgct ttagggttgt tcatcatggc tacggggctt
     6361 ttttccctgg ttctgctgcc cgcgtcacct gcggatatca atattatctg gccgatgatc
     6421 ttatgtggcg ctggatttgg cttattccag tcacccaata accacaccat tattacctcc
     6481 gcctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac ggctcgtcta
     6541 ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt tggtgataat
     6601 ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc ctgtgtcagt
     6661 ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga tcaggacgcg
     6721 ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag cttcaatacg
     6781 tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact taccgttaat
     6841 gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca ggcgctgcag
     6901 cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg catggaattc
     6961 acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca ccagttccag
     7021 aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac cttcatcacg
     7081 atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa agataacgaa
     7141 ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg cgatgtggct
     7201 gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca gcaaaccggt
     7261 gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt tgatgtccgg
     7321 cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct gggttgctac
     7381 agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt ccccgccaac
     7441 agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac caaaaccgaa
     7501 cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg tcaggctcag
     7561 taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg cgattcgcat
     7621 cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca catcgtatgg
     7681 gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac acaactttac
     7741 attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc acaatttctt
     7801 gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc tttttcaagc
     7861 ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg ctcctggtaa
     7921 tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt aaagcgttcg
     7981 ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg cacagcgtag
     8041 ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat atccttcatg
     8101 atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag cagctccggt
     8161 tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct atcccacggc
     8221 agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt cggcatgatg
     8281 atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata gaatttctgg
     8341 atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg atcgtaaata
     8401 cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc acccacaacc
     8461 agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga agcactgaaa
     8521 cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt atcgctgtct
     8581 tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg aataaacaga
     8641 cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag gtcgatgatc
     8701 acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc gtctgtcaaa
     8761 cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac ggtcttcacc
     8821 gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt tacagtacgg
     8881 gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc cttcggccct
     8941 ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat cggcttgcct
     9001 gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt cgctgccgga
     9061 ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag cactgcgcca
     9121 ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg cggggaaaga
     9181 tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga gaaaacatct
     9241 tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt cagggtttca
     9301 cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa tttgacttta
     9361 ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt gctgcggtca
     9421 aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga caaagcgtac
     9481 tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa aacgtcgagt
     9541 ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg ttcaacatcg
     9601 cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc aaagattttg
     9661 gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa gcgcgacgtt
     9721 acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat ttgatcagca
     9781 cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc agctaacgcg
     9841 gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca ccaggtgttc
     9901 tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt ctttggtgat
     9961 ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg cttgtccgac
    10021 agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg cttttactgt
    10081 ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc cttctttgcg
    10141 gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt tctcaccagc
    10201 agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct gaacacgcgc
    10261 tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca gctcaccgca
    10321 tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa gataacgcca
    10381 gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt tcatttcccc
    10441 ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg cttcaccttc
    10501 cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt tactgctatt
    10561 caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc aacaagcgca
    10621 ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat ccgttgatta
    10681 tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt tttcaagctg
    10741 tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga agacagtact
    10801 atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa caatttcaga
    10861 attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct cgatacgctg
    10921 cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta tccggacaca
    10981 ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca aataaaaacc
    11041 tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg caagcgttgc
    11101 cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat ccgcgtaaaa
    11161 ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc agcctattaa
    11221 aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa gattaatgtt
    11281 aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac gccgacaaaa
    11341 aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg
    11401 caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc ctattgtccg
    11461 cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct ggcggcaatg
    11521 gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt gcatatctgg
    11581 ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat gaccaaacag
    11641 ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc cctcattctg
    11701 gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt acgtccggta
    11761 ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct ggtcggcatt
    11821 ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt cggcttgttc
    11881 tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgttgaggag
    11941 ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc
    12001 tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc gcgttgccat
    12061 atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc gctgttagcg
    12121 tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta tctgaatggt
    12181 ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag caacattgcg
    12241 gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg
    12301 ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg
    12361 ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt tgtcatatct
    12421 ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg
    12481 ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct ggacagccgc
    12541 ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa
    12601 aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa tgattgccgg
    12661 ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc
    12721 ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg aagtcggaac
    12781 agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa
    12841 gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga cgccaaaagc
    12901 atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata tcggcatgat
    12961 gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac cgaaatatcg
    13021 gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt cgctgaacag
    13081 cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact atgccatcaa
    13141 tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt
    13201 gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca gtatcagtgg
    13261 cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg ccttcgattc
    13321 acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagatctggc
    13381 ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag gattaaccgc
    13441 cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta aactggattt
    13501 aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg tgaaaatacc
    13561 cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag cgccctgctg
    13621 accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga gttcgttgtt
    13681 gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac cctgacagca
    13741 ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt gcaggtaggc
    13801 caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc catcgagcct
    13861 cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg tgtcgacgtg
    13921 aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg gattaacggc
    13981 gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc actgtatgcc
    14041 aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac catgagtttg
    14101 agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg taaatttgaa
    14161 gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga tctgcacatt
    14221 aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga aggcggggcg
    14281 aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc cagagcatta
    14341 aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg taaaggcgac
    14401 aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca gattacgctt
    14461 cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct cggtattgat
    14521 atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca ggcaaaggcg
    14581 gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt ctcagtggtc
    14641 acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca gccgtatatc
    14701 aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga ggccaccatt
    14761 actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga agccggttcg
    14821 ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt taccggaatg
    14881 acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa acgctatcaa
    14941 cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct ggactttggt
    15001 ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg cggcatcgcc
    15061 ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa acacttcctg
    15121 ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc caaataatgc
    15181 ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc tgtttttttg
    15241 ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt ttctgacaca
    15301 aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg cctgtcagcg
    15361 cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg atttcctgca
    15421 attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag aaggtttctg
    15481 gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc atttaagtgg
    15541 cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt ttgctgacgg
    15601 taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa cgacgcaaat
    15661 tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg ccagttgggt
    15721 aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc tcacacattt
    15781 tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt tgctggacgc
    15841 tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa actggtcacc
    15901 cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg cctttcatgc
    15961 attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg aagaaaacga
    16021 agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt taccacttgg
    16081 cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc atgttttccc
    16141 acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc aggcgatccc
    16201 cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg tgaaagatcg
    16261 cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg atggaaacct
    16321 gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg aagccctgat
    16381 cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata acaaaggtta
    16441 tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa acgcttttga
    16501 actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc cgcaagccgc
    16561 gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt tagccaaacg
    16621 gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg ggaaactttt
    16681 taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca cattcgccgg
    16741 cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca gcagctaaat
    16801 ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg acgacggcga
    16861 attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat ctgaccccga
    16921 tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc tgctaaatgg
    16981 cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct ggattatgcg
    17041 tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg gttattcccc
    17101 cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga tgaaatgttc
    17161 gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt tgtggatgtc
    17221 tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg ccatggcagt
    17281 tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga gtatggtgat
    17341 ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac cggtatcaat
    17401 ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa accagaaatg
    17461 atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc ggctccacaa
    17521 gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DAZSNG010000085        17407 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZSNG010000085 DAZSNG010000000
VERSION     DAZSNG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.086"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHW4Z_002907"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHW4Z_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR8882462.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHW4Z_002908"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHW4Z_002908"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR8882463.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHW4Z_002909"
     CDS             2826..3980
                     /locus_tag="ACHW4Z_002909"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR8882464.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHW4Z_002910"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHW4Z_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR8882465.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHW4Z_002911"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHW4Z_002911"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR8882466.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHW4Z_002912"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHW4Z_002912"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR8882467.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHW4Z_002913"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHW4Z_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR8882468.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHW4Z_002914"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHW4Z_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR8882469.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHW4Z_002915"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHW4Z_002915"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR8882470.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHW4Z_002916"
     CDS             11296..11511
                     /locus_tag="ACHW4Z_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR8882471.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHW4Z_002917"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHW4Z_002917"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR8882472.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHW4Z_002918"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHW4Z_002918"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR8882473.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHW4Z_002919"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHW4Z_002919"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR8882474.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHW4Z_002920"
     CDS             complement(16247..16786)
                     /locus_tag="ACHW4Z_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR8882475.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHW4Z_002921"
     CDS             complement(16802..17320)
                     /locus_tag="ACHW4Z_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8882476.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4042 a         4320 c         4834 g         4211 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cttgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagat
//
LOCUS       DAZSNG010000086        17387 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZSNG010000086 DAZSNG010000000
VERSION     DAZSNG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17387
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.087"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(43..774)
                     /gene="rlmB"
                     /locus_tag="ACHW4Z_002922"
     CDS             complement(43..774)
                     /gene="rlmB"
                     /locus_tag="ACHW4Z_002922"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR8882477.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(954..3395)
                     /gene="rnr"
                     /locus_tag="ACHW4Z_002923"
     CDS             complement(954..3395)
                     /gene="rnr"
                     /locus_tag="ACHW4Z_002923"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR8882478.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="ACHW4Z_002924"
     CDS             complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="ACHW4Z_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR8882479.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4064..5362)
                     /gene="purA"
                     /locus_tag="ACHW4Z_002925"
     CDS             complement(4064..5362)
                     /gene="purA"
                     /locus_tag="ACHW4Z_002925"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR8882480.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5466..5663)
                     /locus_tag="ACHW4Z_002926"
     CDS             complement(5466..5663)
                     /locus_tag="ACHW4Z_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR8882481.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="ACHW4Z_002927"
     CDS             complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="ACHW4Z_002927"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR8882482.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="ACHW4Z_002928"
     CDS             complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="ACHW4Z_002928"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR8882483.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="ACHW4Z_002929"
     CDS             complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="ACHW4Z_002929"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR8882484.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="ACHW4Z_002930"
     CDS             complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="ACHW4Z_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR8882485.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="ACHW4Z_002931"
     CDS             complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="ACHW4Z_002931"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR8882486.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="ACHW4Z_002932"
     CDS             complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="ACHW4Z_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR8882487.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="ACHW4Z_002933"
     CDS             complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="ACHW4Z_002933"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR8882488.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="ACHW4Z_002934"
     CDS             complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="ACHW4Z_002934"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR8882489.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="ACHW4Z_002935"
     CDS             complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="ACHW4Z_002935"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR8882490.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15982..17121
                     /gene="queG"
                     /locus_tag="ACHW4Z_002936"
     CDS             15982..17121
                     /gene="queG"
                     /locus_tag="ACHW4Z_002936"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR8882491.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17104..17157)
                     /locus_tag="ACHW4Z_002937"
     CDS             complement(17104..17157)
                     /locus_tag="ACHW4Z_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882492.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3944 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
       61 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      121 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      181 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
      241 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      301 cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt
      361 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc
      421 ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
      481 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
      541 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      601 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
      661 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      721 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
      781 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
      841 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
      901 acaaagcgca ttttgtcagc aatctaaccc tcttctttta aagagggtat tgatcactct
      961 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
     1021 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
     1081 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acaacgcttg
     1141 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
     1201 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
     1261 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
     1321 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
     1381 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
     1441 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
     1501 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
     1561 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
     1621 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
     1681 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
     1741 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
     1801 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
     1861 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
     1921 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
     1981 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
     2041 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
     2101 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
     2161 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
     2221 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
     2281 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
     2341 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
     2401 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
     2461 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
     2521 ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
     2581 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
     2641 acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt
     2701 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
     2761 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
     2821 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
     2881 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
     2941 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
     3001 tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
     3061 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
     3121 tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
     3181 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
     3241 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
     3301 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
     3361 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
     3421 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
     3481 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
     3541 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
     3601 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
     3661 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
     3721 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
     3781 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
     3841 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
     3901 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
     3961 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
     4021 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
     4081 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
     4141 cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
     4201 ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
     4261 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt
     4321 aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga
     4381 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
     4441 tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc
     4501 cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt
     4561 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
     4621 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
     4681 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
     4741 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
     4801 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
     4861 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
     4921 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
     4981 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa
     5041 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
     5101 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
     5161 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
     5221 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
     5281 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
     5341 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
     5401 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
     5461 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
     5521 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
     5581 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
     5641 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
     5701 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
     5761 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
     5821 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
     5881 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata
     5941 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
     6001 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
     6061 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
     6121 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
     6181 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
     6241 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
     6301 tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt
     6361 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
     6421 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
     6481 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
     6541 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca
     6601 ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
     6661 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
     6721 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
     6781 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
     6841 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
     6901 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
     6961 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
     7021 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
     7081 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
     7141 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggaat
     7201 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
     7261 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
     7321 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
     7381 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
     7441 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
     7501 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
     7561 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
     7621 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
     7681 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
     7741 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
     7801 cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
     7861 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
     7921 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
     7981 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
     8041 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
     8101 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
     8161 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
     8221 agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
     8281 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
     8341 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
     8401 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
     8461 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
     8521 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
     8581 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
     8641 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
     8701 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
     8761 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
     8821 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
     8881 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
     8941 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
     9001 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
     9061 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
     9121 gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca
     9181 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
     9241 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
     9301 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
     9361 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
     9421 tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9481 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg
     9541 ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa
     9601 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9661 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9721 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9781 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9841 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
     9901 tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
     9961 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10021 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10081 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10141 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10201 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10261 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10321 ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg
    10381 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    10441 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10501 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10561 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10621 agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataaggacag aatcaacgct
    10681 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10741 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10801 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10861 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    10921 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    10981 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11041 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11101 caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11161 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg
    11221 tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc
    11281 catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11341 gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg
    11401 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    11461 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag
    11521 ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt
    11581 ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt
    11641 gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat
    11701 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11761 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc
    11821 ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    11881 tttccgccag tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat
    11941 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    12001 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    12061 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt
    12121 catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12181 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg
    12241 tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12301 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12361 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    12421 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    12481 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12541 tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12601 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12661 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12721 gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt
    12781 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12841 cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc
    12901 gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    12961 accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt
    13021 cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt
    13081 gctgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg
    13141 aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13201 cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac
    13261 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13321 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac
    13381 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13441 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13501 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    13561 gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    13621 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    13681 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca
    13741 ccagattatt gccgctgaac aacaacggca gtccctgaat catgcccgtt tgtttgatat
    13801 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13861 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    13921 cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca
    13981 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14041 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14101 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14161 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14221 acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    14281 tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg
    14341 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    14401 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    14461 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14521 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14581 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14641 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca
    14701 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14761 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    14821 gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg
    14881 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    14941 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15001 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15061 tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc
    15121 agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc
    15181 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15241 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15301 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta
    15361 acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga
    15421 tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15481 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15541 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15601 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15661 gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca
    15721 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15781 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15841 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    15901 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    15961 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16021 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16081 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16141 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg
    16201 cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16261 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16321 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16381 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16441 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16501 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16561 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16621 gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa
    16681 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16741 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    16801 cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg
    16861 gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca
    16921 ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag
    16981 cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat
    17041 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa
    17101 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17161 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta
    17221 acttatttat caataggtta aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa
    17281 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    17341 ccagcaataa cgcatcgcgt cgctgtagat attttattga gagaaga
//
LOCUS       DAZSNG010000087        17091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZSNG010000087 DAZSNG010000000
VERSION     DAZSNG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.088"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            85..1224
                     /gene="arnB"
                     /locus_tag="ACHW4Z_002938"
     CDS             85..1224
                     /gene="arnB"
                     /locus_tag="ACHW4Z_002938"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR8882493.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1228..2196
                     /gene="arnC"
                     /locus_tag="ACHW4Z_002939"
     CDS             1228..2196
                     /gene="arnC"
                     /locus_tag="ACHW4Z_002939"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR8882494.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2196..4177
                     /gene="arnA"
                     /locus_tag="ACHW4Z_002940"
                     /pseudo
     CDS             2196..4177
                     /gene="arnA"
                     /locus_tag="ACHW4Z_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4174..5064
                     /gene="arnD"
                     /locus_tag="ACHW4Z_002941"
     CDS             4174..5064
                     /gene="arnD"
                     /locus_tag="ACHW4Z_002941"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR8882495.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5064..6716
                     /gene="arnT"
                     /locus_tag="ACHW4Z_002942"
     CDS             5064..6716
                     /gene="arnT"
                     /locus_tag="ACHW4Z_002942"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR8882496.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6713..7048
                     /gene="arnE"
                     /locus_tag="ACHW4Z_002943"
     CDS             6713..7048
                     /gene="arnE"
                     /locus_tag="ACHW4Z_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR8882497.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7048..7434
                     /gene="arnF"
                     /locus_tag="ACHW4Z_002944"
     CDS             7048..7434
                     /gene="arnF"
                     /locus_tag="ACHW4Z_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR8882498.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7428..7700)
                     /gene="pmrD"
                     /locus_tag="ACHW4Z_002945"
     CDS             complement(7428..7700)
                     /gene="pmrD"
                     /locus_tag="ACHW4Z_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR8882499.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7805..9160)
                     /gene="menE"
                     /locus_tag="ACHW4Z_002946"
     CDS             complement(7805..9160)
                     /gene="menE"
                     /locus_tag="ACHW4Z_002946"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR8882500.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9157..10119)
                     /gene="menC"
                     /locus_tag="ACHW4Z_002947"
     CDS             complement(9157..10119)
                     /gene="menC"
                     /locus_tag="ACHW4Z_002947"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR8882501.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10119..10976)
                     /gene="menB"
                     /locus_tag="ACHW4Z_002948"
     CDS             complement(10119..10976)
                     /gene="menB"
                     /locus_tag="ACHW4Z_002948"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR8882502.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10991..11749)
                     /gene="menH"
                     /locus_tag="ACHW4Z_002949"
     CDS             complement(10991..11749)
                     /gene="menH"
                     /locus_tag="ACHW4Z_002949"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR8882503.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11746..13416)
                     /gene="menD"
                     /locus_tag="ACHW4Z_002950"
     CDS             complement(11746..13416)
                     /gene="menD"
                     /locus_tag="ACHW4Z_002950"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR8882504.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13505..14800)
                     /gene="menF"
                     /locus_tag="ACHW4Z_002951"
     CDS             complement(13505..14800)
                     /gene="menF"
                     /locus_tag="ACHW4Z_002951"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR8882505.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="ACHW4Z_002952"
     CDS             complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="ACHW4Z_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR8882506.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15239..15700)
                     /locus_tag="ACHW4Z_002953"
     CDS             complement(15239..15700)
                     /locus_tag="ACHW4Z_002953"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8882507.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15765..16682
                     /gene="rbn"
                     /locus_tag="ACHW4Z_002954"
     CDS             15765..16682
                     /gene="rbn"
                     /locus_tag="ACHW4Z_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR8882508.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16873..17061
                     /locus_tag="ACHW4Z_002955"
                     /pseudo
     CDS             16873..17061
                     /locus_tag="ACHW4Z_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         3983 a         4627 c         4344 g         4137 t
ORIGIN      
        1 atttcgctcc aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg
       61 tattcaatgg cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg
      121 ggcgtggagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
      181 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
      241 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      301 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
      361 ctgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      421 gcgatcgaat cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt
      481 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      541 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      601 accgctattt tttcatttca tgccattaaa aatattacct gtgctgaagg tggcctgatt
      661 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt
      721 gtcgatgcct atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg
      781 ggctataagt acaatctgac cgatattaac gccgcgattg ccctgacaca gttagtcaaa
      841 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
      901 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      961 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
     1021 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
     1081 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1141 tgttcgttgc cgctgttccc ggatatgact accgccgatg ccgaccgcgt catcacagcc
     1201 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1261 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     1321 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     1381 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     1441 attttgatta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     1501 actggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
     1561 cgcctggtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     1621 caggacagct ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc
     1681 actggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
     1741 gatgcgatgt tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc
     1801 gcccgccgtg caattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     1861 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1921 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     1981 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     2041 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc
     2101 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2161 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2221 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     2281 tttttaccca taccgataat cccggtgaaa aagcctttta tggttcggtg gctcatctgg
     2341 cggcggaaag agacattccg gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2401 aacgcattgc ccaactgtca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2461 gcgacgagat ttttcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     2521 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg
     2581 gcgttacatt gcaccgaatg gtaaacgtgc cgatgctggg gccattgtgg cgcaactgcg
     2641 cgttgccatt gcgccagacg atatcgctat tacgctgcat cataaattgt gccatgccgc
     2701 gcgccagcta ctggaacaga cattacccgc cattaaacac ggtaatattc tggaaatcgc
     2761 ccagcgcgaa aacgaagcca cctgttttgg tcgcagaacg ccggatgaca gtttccttga
     2821 atggcataaa ccggcatccg tactgcacaa catggtacgt gccgttgccg atccgtggcc
     2881 gggtgccttc agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc
     2941 tcatgccagc aaagcacagc cggggagcgt gatttctgtt gcgccactgc tgattgcctg
     3001 tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg
     3061 ctcgcaatta gcgcagatgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc
     3121 ctgcaccgcc cgacgccgta cccgggtact catcctcggg gtgaatggct ttattggcaa
     3181 ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatattgg
     3241 cagcgatgcg ataagccgtt ttctgaatca tccacatttt cactttgtcg aaggcgatat
     3301 cagtattcat tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg tcttgccgct
     3361 ggtggcgata gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga
     3421 ttttgaagag aatctgcgca ttatccgcta ctgcgttaag taccgtaagc gaatcatctt
     3481 cccgtcaact tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggaccattc
     3541 taatttaatc gtcggcccgg tgaataaacc acgctggatt tattcggtgt caaaacaatt
     3601 acttgatcgg gtgatctggg cctatggcga aaaagagggt ttacagttca ccctcttcct
     3661 cccgtttaac tggatgggac cacgactgga taaccttaat gcagcgcgaa ttggcagctc
     3721 ccgcgctatt acgcaactca ttctcaatct ggtagaaggt tcaccaatta agctaattga
     3781 tggcggaaaa caaaaacgct gctttactga tatccgggac ggtatcgagg cgttgtacca
     3841 cattatcgaa aatgcgggaa atcgctgcga cggcgaaatt atcaacattg gcaatcctga
     3901 gaacgaagcg agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc
     3961 gctgcgccat catttcccac cgtttgcggg ctttcgcgtt gtcgaaagta gctgctacta
     4021 cggcaaagga tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccaccgctg
     4081 cctggactgg gagccgaaaa ttgatatgca ggaaaccatc gacgaaacgc tggatttctt
     4141 cctgcgcacc gttgatctta cggataaacc atcatgacca aagtaggctt acgcattgat
     4201 gtcgatgcct ttcgtggcac ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag
     4261 cataatattc aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc
     4321 tggcgactgg tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc ggcatcgctt
     4381 tatggctggg atattttact ggcaggtacg gcctggccag gtaaagagat tggtcatgcc
     4441 aatgccgata tcattcgtga agcggctaaa catcacgaag tcggtctgca cgcctgggat
     4501 caccatgcct ggcaagcccg tagcggtaac tgggatcggc aaacaatgat cgacgatatt
     4561 gcacgcggtc ttcgcactct ggaagagatt atcggtcaac cggtaacctg ttctgccgct
     4621 gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac
     4681 aacagcgatt gtcgtggggc cataccgttc cgtccactgc ttgaatcagg aaaccctggc
     4741 actgcgcaaa ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa
     4801 gcagaagatt ttaacggttg gttactcaac cgcatcctgc gagataaagg cacgccggtt
     4861 tataccattc atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctc
     4921 aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagagacg
     4981 ttgccgctcg gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc
     5041 caacaaattg cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc atcttcgcgt
     5101 ttattgcctg ctattacctg ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa
     5161 cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg cgactggatt gtgccccatc
     5221 tgttagggct acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc
     5281 aatggctatt tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt
     5341 taactgccgc gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc
     5401 tactcgccac agtaatttat ctctcattgt ttattgtcta tgccatcggc acttatgccg
     5461 tgctcgatcc gtttattgca ttctggctgg tggcgggaat gtgcagcttc tggctggcaa
     5521 tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt actgctggga atcacctgcg
     5581 gcatgggggt gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgctgc
     5641 catgggtagc gacgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta
     5701 tcagttgcgt actgacggtt ctcccctggg gactggcgat agcgcagcgg gagcctgact
     5761 tctggcacta ttttttctgg gttgagcata ttcaacgctt tgcactagat gatgcccaac
     5821 atagagctcc gttctggtac tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg
     5881 gattactccc cggtgcactg tacacaggct ggaaaaaccg caagcattcc gcaaccgtct
     5941 atttgttgag ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggcaaattgc
     6001 ccacctatat tctttcttgc tttgcacctc tggcaatgct gttggcgcat tacgcgttgc
     6061 tggcagcaaa aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg
     6121 gcgtcactgg cattattgcc acatttgtga tctccccctg gggaccaatg aacacgccgg
     6181 tgtggcaaac cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg
     6241 catttttcgg ctggtacacc ttaacaaacg tcgaaaagac ctggtctttc gccgcgcttt
     6301 gcccgctggg gctggcgttg ctggtaggat tttcaattcc tgacagagtc atggaaggaa
     6361 aacatccgca attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc
     6421 ttactgatag cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca
     6481 tcatgtatcg ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga
     6541 gatttgtcag cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta
     6601 ttacactcgt gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc attccgcccg
     6661 cagatgccat cgttcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg
     6721 gctaacatta gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc
     6781 aacctgcttt gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc
     6841 gctggcttgt cttggtcttg ccatgatgct ctggctgctg gtcttgcaga acgtaccggt
     6901 aggcattgcc tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa
     6961 actgtggcac gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg
     7021 cattgtgatc ctcgggagta cggtgtaatg tgcctgatat ggggattatt cagcgtcatt
     7081 attgcctcag ttgcgcaatt aagcctgggt tttgcggcaa gtcatctgcc gccgatgacg
     7141 cacttatggg attttatcgc ggctctgtta gcttttggcc tggatgcccg gatactgtta
     7201 ctcgggctgc tgggttatct gctttccgtt ttttgttggt ataaaacgct acataaactt
     7261 gcgctaagca aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca
     7321 atggtacttc ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt
     7381 attatgagcg ggttgatgct gattttcctg cccacgacaa aacaacgtta ctgagttttc
     7441 cctgccgctg tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact
     7501 ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt
     7561 aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg
     7621 ccagcatcgc agagcataag tacatgtctg ttatcttgtt tattgcagca cgattttttg
     7681 tgccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     7741 aacagataaa ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg
     7801 ttaattattg ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc
     7861 cgtttttcag ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct
     7921 tatctttcac ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg
     7981 gccgatgacc aaactccttg tcggcaacgg aaacgataaa cacctgcaaa accgcaggat
     8041 gtgcagcaat aacacgctcg acttcttccg gctgaatacc ctcaccgcca ctgaagaaca
     8101 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8161 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     8221 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     8281 gcagcggcaa accaacgtct gccaggccgt cggcttcttt cgcacatacc gtggaggcaa
     8341 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     8401 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt
     8461 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca
     8521 tttgctccaa tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc
     8581 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
     8641 cgccaaacgg aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat
     8701 ggacagcggc tttcggcaaa ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg
     8761 tcggctgcca cgcagcggca tgtacgcctt caaccagctg aatgtgcagc gacgttaatg
     8821 ccggaaacgt gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca
     8881 attcttcaag caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt
     8941 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     9001 cgctgccctc aaccaccccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac
     9061 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9121 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaactgct cctgtacatc
     9181 agcgtccact aacggcagtg ggctccccgg ccagcgacga acctgctgcg cctgcatcag
     9241 atccagcgtg tccagtcccg gaatggtgtc tggcgttaac caggcagcaa tccgcgccag
     9301 ttgcgttaga cctaagctcg actcaatgga agaactgatc accgccgtca gccccagcgc
     9361 atgcgccgcc tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat
     9421 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc
     9481 atcccaggca atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt
     9541 gcacggctct tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa
     9601 ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
     9661 aattgcctcc agcaacagat tcaccaccat gccgtcgcgc accgcttcgt acaatcccac
     9721 tttgaccttc gccactttct cgcccggcat atcggcaagt ttgaggatca gatcgtccgg
     9781 atcgccatta cacagcggtg ccgcacggta gttggctgct tgcggcaacg tatctgtcag
     9841 ttctgccaat gcacagctta cgccaaaggc cacggaaggc atctgcggta gctcgcaatc
     9901 gcctgccagc cagttattta cccaggcaag cagcacgctt tgcgcctctt cccagttttc
     9961 ctggctgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag
    10021 gcaaacgtac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc
    10081 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10141 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    10201 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag
    10261 tcggcgttca gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg
    10321 cggcaccaac ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    10381 cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca ggaaccagat ttctcgcgct
    10441 tttttctgcc cgacgatacg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10501 actttcgggc cagtctgacc gaagatggca ttatctgctg cgatagtcag gtcgcacatc
    10561 atgtgcagaa cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10621 ggacaggtac ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca
    10681 tctttatagc cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta
    10741 tcgcctgcgc cagtcagaat gatcacgccg atattgtcgt cataacgcgc atctgccagt
    10801 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10861 atggtgattt ttgcgatacc gtcggtggat ttttcataac gaatgtcctc gaaaccttcg
    10921 gagcagtcgt gccattcaac cggtgcgtaa ggcattgctt catcaggata aatcatattg
    10981 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt
    11041 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    11101 ccagggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11161 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    11221 caagggttgc gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat
    11281 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    11341 tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat
    11401 tttgcagccc cggatgcccg ccttcgacaa taaccccaca aagccccgcc agcccctggc
    11461 aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga
    11521 tgttgtaact aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    11581 taatcgccgc cgaaccacca tgacctagga gatcaacata caaccgtgag tagtcggcaa
    11641 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    11701 gccagggtaa acctagtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11761 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11821 caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt
    11881 ttgcggatga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11941 cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg
    12001 cccgccgttg ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc
    12061 cagcgcgttg agatcgtaaa gcgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12121 gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg
    12181 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    12241 caggctgtta ccaacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc
    12301 cagttgcgct tcgccaaacg catcacggcg ggcaataacc gcctgcattg cctgttccgc
    12361 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12421 gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc
    12481 aatgtcatca acaatccagt actcttctgg ttcacagctt gcctgccatt gcaggagccg
    12541 tttccccgtc aggctacttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt
    12601 cgctttggca ttgcctaacc aaagatcggc acacggcagc ggctgcccgg tttgtgacag
    12661 cacatcgcca atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc
    12721 ttccgcactc atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc
    12781 gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12841 ccagtcaccc agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg
    12901 ttcagcaaac gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc
    12961 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
    13021 gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc
    13081 aattagctcc ggcgggcgat cggcggttaa gagaatcagt ttttctccgg ttaacccggc
    13141 ttcaatcagt gccggataga gatttgccac cgccgtgccg gaggtcacaa tcaccgccac
    13201 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
    13261 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13321 cgagcctggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13381 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13441 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13501 attactattc catttgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
    13561 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    13621 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat
    13681 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    13741 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg cagcaactga tgtaaacaga
    13801 tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    13861 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt
    13921 ggcagatatc ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc
    13981 actctcctaa ctgctgcgcc tgcttatcat caggatgatt tgctactgtt cccgccagcg
    14041 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    14101 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    14161 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    14221 cgagcaccac tttatcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    14281 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    14341 gcaagggctt gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct
    14401 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    14461 attccaggcg gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa
    14521 tgcgtaagtc agcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14581 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14641 agaattgcgg gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14701 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14761 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14821 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14881 aacggcgtgc cagcaacagt cctagtacca gcccgacggc cgcgcccaca ccaattcctt
    14941 gccagggttt ttcgtggacg tagtcatcag cacgataaac agcctgcttc gcccgatagt
    15001 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcat
    15061 gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt
    15121 ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    15181 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaaga gttgttgatt
    15241 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    15301 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    15361 ggtaaacagg cttatcaggc cagtgatgcg tacatgtttc gagtgtttta ctcatcagct
    15421 gctgccccac tttttcacca cgcaacgctt cactgacaat cacccgacct ataacgaccg
    15481 gctcaagatc atcatcactt ttcagaatcc tcgcatacgc caccagttca tcatttttcc
    15541 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    15601 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    15661 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    15721 atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    15781 taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    15841 tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc
    15901 agctactgca taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc
    15961 atggcgatca tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    16021 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    16081 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    16141 ttctcgatga tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt
    16201 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    16261 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    16321 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    16381 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    16441 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    16501 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggcaagc
    16561 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcaacac ctgttacgtg
    16621 aatgcaggtc aattttcccg gcgactgaac tggcgaatga tttcaccgtg tttaacgttt
    16681 aactctgctt attattaaaa acagggtgaa acttgccctg ttatagcaac ccgcgctaca
    16741 tatatccacc cacacaggca tattgagcga tttttgatat ttattcatct ccttacgcac
    16801 tatattcaga gcaataagtt gcctcttttc atcttgattc agcacaacct ttgcaactaa
    16861 aaggagccca tgatggttac agttgtcagc aattattgtc aattatctca aacccaactc
    16921 agtcagacat ttgcagaaaa atttactgtg accgaggaat tactgcagtc tttaaaaaaa
    16981 acagcgttat ccggagatga agaaagtatt gagttactgc ataatattgc gttaggttat
    17041 gatgaatttg gaaaaaaagc tgggtaatgc tgccaactta ctgatttagt g
//
LOCUS       DAZSNG010000088        17091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZSNG010000088 DAZSNG010000000
VERSION     DAZSNG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.089"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..510
                     /locus_tag="ACHW4Z_002956"
     CDS             <1..510
                     /locus_tag="ACHW4Z_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR8882509.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHW4Z_002957"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHW4Z_002957"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR8882510.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHW4Z_002958"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHW4Z_002958"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR8882511.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHW4Z_002959"
     CDS             complement(2878..3075)
                     /locus_tag="ACHW4Z_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8882512.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHW4Z_002960"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHW4Z_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR8882513.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHW4Z_002961"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHW4Z_002961"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR8882514.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHW4Z_002962"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHW4Z_002962"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR8882515.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHW4Z_002963"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHW4Z_002963"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR8882516.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHW4Z_002964"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHW4Z_002964"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR8882517.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHW4Z_002965"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHW4Z_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHW4Z_002966"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHW4Z_002966"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR8882518.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHW4Z_002967"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHW4Z_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR8882519.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHW4Z_002968"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHW4Z_002968"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR8882520.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHW4Z_002969"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHW4Z_002969"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR8882521.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHW4Z_002970"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHW4Z_002970"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8882522.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW4Z_002971"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW4Z_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         3993 a         4864 c         4443 g         3791 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgtgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta a
//
LOCUS       DAZSNG010000089        16388 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZSNG010000089 DAZSNG010000000
VERSION     DAZSNG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.090"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHW4Z_002972"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHW4Z_002972"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR8882523.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHW4Z_002973"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHW4Z_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR8882524.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHW4Z_002974"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHW4Z_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR8882525.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHW4Z_002975"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHW4Z_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR8882526.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHW4Z_002976"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHW4Z_002976"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR8882527.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACHW4Z_002977"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACHW4Z_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR8882528.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACHW4Z_002978"
     CDS             5920..6417
                     /locus_tag="ACHW4Z_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR8882529.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACHW4Z_002979"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACHW4Z_002979"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR8882530.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHW4Z_002980"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHW4Z_002980"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR8882531.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACHW4Z_002981"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACHW4Z_002981"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR8882532.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACHW4Z_002982"
     CDS             9102..9665
                     /locus_tag="ACHW4Z_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR8882533.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHW4Z_002983"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHW4Z_002983"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR8882534.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACHW4Z_002984"
     CDS             complement(10257..11237)
                     /locus_tag="ACHW4Z_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR8882535.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACHW4Z_002985"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACHW4Z_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR8882536.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACHW4Z_002986"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACHW4Z_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR8882537.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACHW4Z_002987"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACHW4Z_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR8882538.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACHW4Z_002988"
     CDS             12838..13431
                     /locus_tag="ACHW4Z_002988"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR8882539.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACHW4Z_002989"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACHW4Z_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR8882540.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="ACHW4Z_002990"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="ACHW4Z_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="ACHW4Z_002991"
     CDS             15882..16385
                     /locus_tag="ACHW4Z_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR8882541.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4123 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggagaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgg
//
LOCUS       DAZSNG010000090        16097 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZSNG010000090 DAZSNG010000000
VERSION     DAZSNG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.091"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACHW4Z_002992"
     CDS             <1..588
                     /locus_tag="ACHW4Z_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR8882542.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHW4Z_002993"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHW4Z_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR8882543.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHW4Z_002994"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHW4Z_002994"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR8882544.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHW4Z_002995"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACHW4Z_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHW4Z_002996"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHW4Z_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR8882545.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHW4Z_002997"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHW4Z_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR8882546.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHW4Z_002998"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHW4Z_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR8882547.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHW4Z_002999"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHW4Z_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR8882548.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHW4Z_003000"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHW4Z_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR8882549.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHW4Z_003001"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHW4Z_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR8882550.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHW4Z_003002"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHW4Z_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR8882551.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHW4Z_003003"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHW4Z_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR8882552.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHW4Z_003004"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHW4Z_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR8882553.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHW4Z_003005"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHW4Z_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR8882554.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHW4Z_003006"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHW4Z_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR8882555.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHW4Z_003007"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHW4Z_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR8882556.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHW4Z_003008"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHW4Z_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR8882557.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHW4Z_003009"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHW4Z_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR8882558.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHW4Z_003010"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHW4Z_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR8882559.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHW4Z_003011"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHW4Z_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR8882560.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHW4Z_003012"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHW4Z_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR8882561.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHW4Z_003013"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHW4Z_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR8882562.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHW4Z_003014"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHW4Z_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR8882563.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHW4Z_003015"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHW4Z_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR8882564.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHW4Z_003016"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHW4Z_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR8882565.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHW4Z_003017"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHW4Z_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR8882566.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHW4Z_003018"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHW4Z_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR8882567.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHW4Z_003019"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHW4Z_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR8882568.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHW4Z_003020"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHW4Z_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR8882569.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRVLNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHW4Z_003021"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHW4Z_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR8882570.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHW4Z_003022"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHW4Z_003022"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR8882571.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHW4Z_003023"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHW4Z_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR8882572.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3783 c         4291 g         3882 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctaaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtt gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgttctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaagccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tcttttt
//
LOCUS       DAZSNG010000091        15930 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZSNG010000091 DAZSNG010000000
VERSION     DAZSNG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.092"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="ACHW4Z_003024"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="ACHW4Z_003024"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR8882573.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="ACHW4Z_003025"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="ACHW4Z_003025"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR8882574.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="ACHW4Z_003026"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="ACHW4Z_003026"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR8882575.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="ACHW4Z_003027"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="ACHW4Z_003027"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR8882576.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="ACHW4Z_003028"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="ACHW4Z_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="ACHW4Z_003029"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="ACHW4Z_003029"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR8882577.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="ACHW4Z_003030"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="ACHW4Z_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="ACHW4Z_003031"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="ACHW4Z_003031"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR8882578.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="ACHW4Z_003032"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="ACHW4Z_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR8882579.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8880..8969
                     /gene="yldA"
                     /locus_tag="ACHW4Z_003033"
     CDS             8880..8969
                     /gene="yldA"
                     /locus_tag="ACHW4Z_003033"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR8882580.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8973..10211)
                     /locus_tag="ACHW4Z_003034"
     CDS             complement(8973..10211)
                     /locus_tag="ACHW4Z_003034"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR8882581.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="ACHW4Z_003035"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="ACHW4Z_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR8882582.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="ACHW4Z_003036"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="ACHW4Z_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR8882583.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="ACHW4Z_003037"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="ACHW4Z_003037"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR8882584.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="ACHW4Z_003038"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="ACHW4Z_003038"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR8882585.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="ACHW4Z_003039"
     CDS             14682..15584
                     /locus_tag="ACHW4Z_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8882586.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15930
                     /locus_tag="ACHW4Z_003040"
                     /pseudo
     CDS             15731..15930
                     /locus_tag="ACHW4Z_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3953 a         4119 c         3854 g         4004 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agccccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc
//
LOCUS       DAZSNG010000092        15515 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZSNG010000092 DAZSNG010000000
VERSION     DAZSNG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15515
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.093"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(400..645)
                     /locus_tag="ACHW4Z_003041"
     CDS             complement(400..645)
                     /locus_tag="ACHW4Z_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882587.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1422..2264)
                     /locus_tag="ACHW4Z_003042"
     CDS             complement(1422..2264)
                     /locus_tag="ACHW4Z_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR8882588.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2349..2546)
                     /locus_tag="ACHW4Z_003043"
     CDS             complement(2349..2546)
                     /locus_tag="ACHW4Z_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR8882589.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2566..3054)
                     /locus_tag="ACHW4Z_003044"
     CDS             complement(2566..3054)
                     /locus_tag="ACHW4Z_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR8882590.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(3051..3428)
                     /locus_tag="ACHW4Z_003045"
     CDS             complement(3051..3428)
                     /locus_tag="ACHW4Z_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR8882591.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3475..3852)
                     /gene="cbeA"
                     /locus_tag="ACHW4Z_003046"
     CDS             complement(3475..3852)
                     /gene="cbeA"
                     /locus_tag="ACHW4Z_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFR8882592.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3932..4153)
                     /locus_tag="ACHW4Z_003047"
     CDS             complement(3932..4153)
                     /locus_tag="ACHW4Z_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR8882593.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4240..4716)
                     /gene="radC"
                     /locus_tag="ACHW4Z_003048"
     CDS             complement(4240..4716)
                     /gene="radC"
                     /locus_tag="ACHW4Z_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8882594.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4732..5205)
                     /locus_tag="ACHW4Z_003049"
     CDS             complement(4732..5205)
                     /locus_tag="ACHW4Z_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8882595.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(5547..6365)
                     /locus_tag="ACHW4Z_003050"
     CDS             complement(5547..6365)
                     /locus_tag="ACHW4Z_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8882596.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(6544..6675)
                     /locus_tag="ACHW4Z_003051"
     CDS             complement(6544..6675)
                     /locus_tag="ACHW4Z_003051"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR8882597.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            complement(6776..7231)
                     /locus_tag="ACHW4Z_003052"
     CDS             complement(6776..7231)
                     /locus_tag="ACHW4Z_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFR8882598.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(7307..9823)
                     /locus_tag="ACHW4Z_003053"
     CDS             complement(7307..9823)
                     /locus_tag="ACHW4Z_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882599.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            complement(9944..13066)
                     /gene="agn43"
                     /locus_tag="ACHW4Z_003054"
                     /gene_synonym="flu"
     CDS             complement(9944..13066)
                     /gene="agn43"
                     /locus_tag="ACHW4Z_003054"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFR8882600.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            complement(13400..14272)
                     /locus_tag="ACHW4Z_003055"
     CDS             complement(13400..14272)
                     /locus_tag="ACHW4Z_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFR8882601.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            complement(14382..15401)
                     /locus_tag="ACHW4Z_003056"
     CDS             complement(14382..15401)
                     /locus_tag="ACHW4Z_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8882602.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         3517 a         4033 c         4020 g         3945 t
ORIGIN      
        1 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
       61 caggctgagg agtccccaga aaagaagaca ggcatagagt tttgtgatct actgattgcg
      121 ctaacaaatt caatgagatc acctctatgc ctgcccgtta agtatgtcag gcttttttcc
      181 gtaatgctct aacttccacg cattggtttc tgacatcaaa taaggctatg actttctctc
      241 ccttttaatc tcattctgat ctaaaaccat atcttaaaaa aatgcatggg tattttaatt
      301 attttgatgt tgatctaacg cactcaagga ccattttatt accgattatc cattctattt
      361 aactgttgcg atataaatgt ttgttttagg tgctttaatt cagtacaagt catcaaaccg
      421 taccagaccc tgactgtcag tgacaccgac aggtgggggc ggcggtgtgg ggtcaaactg
      481 agtgtgtctg cctctggccc acagagccag ctctgccgcc attgcccgtg gatgatactc
      541 cagctgcacg ttgagaccgg aaactgggtt gttgttctca tccacgaact gcagcgccag
      601 ccagtgcatc cgctgcttac cgcaggcgac ttctttgtac ttcattttgt gactcaggca
      661 ggtgactgtc gtgttgcctg ttgcgtccat tttgcggtta aaccctttta attcaatttg
      721 ttaaattgct ttccaccttt tgcggggcgc gacttaacca aaatattcac tgttatcata
      781 atatgcaatg ggctggaggt tgcaggtcat ttagggattt tctgtaagcc ccgttttgca
      841 gaacgttcta tctccgattg agatcctgtc tctcggtatt accggtagtc tggccataat
      901 cccctatgga tcatattgat atagtaggca taacacccaa aagttgacta aagctaaatc
      961 aatcagagtg aaaaatgttc ggtttgatgt tgggggggac tgaggagggg ccaacgcagg
     1021 tggtatgcgt tttacttcag accggacaaa gaccggatgt atccgtgcag taatgaatcc
     1081 gttagccagg tgctcatgtg gaaatagtgt ctattgggga tttgaagttt gacagaaaag
     1141 gccacagtga ccagaagggg gctacttaga agaaatgttc aggctgggtg gaagaggtaa
     1201 agcagtgaca tcagcattca aagtgaccgg atatctacca gaaccccaga aagcaaaaaa
     1261 ccagcccgta ggctggttct tctaaatagt ggtgccggac tcggaatcga aaatttcttt
     1321 tatcatattg atattaatgc aatttgtttg gtttgattaa aaatgtaccc tcaattatac
     1381 ccgcagtccc ttttgatatc gaaagggaat cactctcggg atcaggctga gagcacagaa
     1441 ggaaagtacc tggctattat atcgttaatt ctgtcaatca gttccagcct tttgaaagtg
     1501 atgtgtgcag atcccttctt aaagtattta atgatgaaca tctcgtcttc atagcgctct
     1561 ttaccctgaa cggactggat gtgaccatcc agacgaatag tgatgttttc ccggttgtcg
     1621 gggatcggtt tgccgctgaa cagatgtagc attctttcca ggtcgacgag tcggtcagtc
     1681 tgttgcccgg tgataagatg aaacccccac cggtcccacc tcaccaggtt gttgacgata
     1741 attttactgc caaatttaca gggtgagttg gttttgtaat cccagctcag ccctttgaag
     1801 acgttgatca ctccccgttc aaacacttca tctttgttct ggtgcagttg ttcaaatgtg
     1861 ctcagaatgt tcgcttcgct gatttccgga aaattatcat attccagtga cctgtaccat
     1921 gtgtcccggg tctgcgcatc catcagactc agcatcccgg aacgttgcat cagatcacgc
     1981 cagagttcgc gatcaagatt gcgtgtgatg gctttcattg cggtttccgg tttctccatc
     2041 agccagcaac cgcagcgaaa atcctgtttc atggcccagt ccagagccgt tttacctcta
     2101 atactttctg tcattgctga tacttcagcc agttgtcgga taagtgtgtc gatttgcagc
     2161 aatgtactct ttcgtacagc cagaatgtgg cggatactgg ttgagcagat aacgtccgta
     2221 tgcccgttga ccacttccgg acgagaggct gtgatttcag acataatcga tattcctttt
     2281 tgcagataaa cgaaaacgcc agccgtattc aggctggcgt cagtggaatg aagtctggtg
     2341 agtaagactc accggttctg ttgcagaaac tgcagctgac gcagggtacg gatatcttcc
     2401 ctggcctgtt caatacaggg cagcagtgct gccgaatccg ttttatcctc atcgttgtcg
     2461 aagataatcc ctgattcgca gtcaatattg tcctgcagcc acgcaatcag aatatccagc
     2521 gccgtttctg tggttaataa tttcatccag tgtcttttgt cctgattacc agtcgaaagt
     2581 ggggaacccc ggcagacatt ctgcgccggc gtccagatga atgagactga cgccataacg
     2641 ccgggtgaga gtgacgacca gacgacggaa cgttctggac aggcccaggc gtttaagccg
     2701 cagaaccggg tacgaccatg catcggtacg taacagatag ccgctgcctg taaaatgaat
     2761 ccattctgtt tcgccgtcgt cattagtctg gtgtgacagc gagtacagcc aggcgttgtc
     2821 cgtttccgtg atatgtgcgg tgctgcaacg tatgcaggta agggttgtta acggtggtgg
     2881 tgtactggtt gcgtgttggt cggatttatc gaccacgcgg agtgaataac cgttttcaga
     2941 gaccttgtta atcagttcac tgagattaac accatcgacg tcaacgacaa tgcgccccat
     3001 gttcagtgcc tgcacgttaa cgctgtcggc ttccagcgtc agggccagtt tcattgtttc
     3061 gcttccggat gcttcccccg ggtaatgtta tttaccgttc tgtagttatc gcgggtcatc
     3121 aggccggttg cccggcgtgc ccggagaata tcaatgctgt tgattaactg cgacggggct
     3181 cctgcgctga atcccggctg gtcggtacgt accagcgcgt atttttcaac gagaaagttc
     3241 accgcgtcac acagtgaaat tcctgcctca atatgctgct caatcacacg ttcatcagcg
     3301 aacggtgtgt cattcagcgt gaggccatag tgctggtcca gcagtcgggt gagcagtgtc
     3361 tgccagatgg tgatgggaga cgggcagccc gatgcctccc gtacgtgagt gtcgggtaat
     3421 gtattcatct taagttctct gtaacaggtg gttataaagg ggagatatcc ggtgttatac
     3481 ggtggttgcg ggcgctgccg gtgtcggata aacagccaga taaacgtaac cacaggagcc
     3541 gagggtgtcg gcttcgcagg tcagcccttt cgcatacagt gtgacggtat gctgatggcg
     3601 gggattcagt tcaccgccag tgagcatgag ttccagttgt ttcatcagca gcggaaaggc
     3661 ctggtccagg tggtacgcat ctacgtcgct gaacctgcct ctgataccgg cgcggtctgc
     3721 aaggtaatgc aggcggttac cctcctgtac cagtcgggcc ccgaaacagg gcgtcacggt
     3781 gcagggcagc ccccaccagg ggcggtcgtg attgtcgtcg ggaggcgttg tcccggggag
     3841 tgtgtctgac acgataaagt cctcacagaa aatcggttaa gaatggtccg gtagtggcga
     3901 taattctgct catcagaatt cccttccaga ctcagttcag ggtgacgctc atcagccgga
     3961 catacggacc aaaactgtcc ttacggcgtt ctgcaaacac agccagtaca ccgggaatat
     4021 cctgcacttc acggccggta tatgtatccg tgctaccgtg ccagcggtat ttaccggtac
     4081 agaacggaaa aagacgggat gcaggatgct gacggtgaat acgcatggct tcaccacggg
     4141 tgatgatttt catgagaata gtcctcctga atgtgaaagt aagaaacagc tgagaaagag
     4201 aaactgaagt gatatgaagg tgattgtgac ggtgacgggt taaagcagac cgtgttccgc
     4261 aaaggagaaa acctggctgc caccgactat cagatggtcc ggtacccgga tatcaaccag
     4321 ggccagtgcc tgtaccagac gctcagtgat aaggcggtct gccttgctgg gggggacttc
     4381 accggacggg tgattgtgcg ccagcaccac ggcagcggca ttgtggtaca gggcgcgttt
     4441 aatcacttcc cggggatgga cttccgtacg gttgatggtg ccggtgaaga gggtttcacc
     4501 ggcaatcagc tgattctggt tgttcagata cagcacccgg aactcttcac gctccagtcc
     4561 cgccatgttc agaatcagcc attcccgtgc cgcatgcgtg gaggtgaagg ccacgccggg
     4621 ttcatgaaga tggcggtcca gggttttcag ggcccgcaga atgaggctgc gctctctggt
     4681 tgtcatttct ccgggcagaa aggaaagctg ctgcattgtc cctgccacca ttcagtcgat
     4741 gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca gccggtaata
     4801 atggaccgtc atggcataac actccgtacg acaggcatga tgactgtacg tcatcagaca
     4861 ggcagcaatg ccggcagctt ccggactcat ttcagcgcgg ttaccgttca tggcattgaa
     4921 cagtacccat gtttcgtcat catcgtcatc cggttcgggt gccataaatg ccccgccgtt
     4981 gttcagggtg tacagattcc agataccacc gcagtagtct tcgcacagac ggtccatcca
     5041 gccgaagaca cggggctcca gggtcaccca ctgtggaatg aggccaaagt gctgcggcca
     5101 gaagctgatg cgctgttcat cagggacgag ggtggcaacc agttgttgca ggtcagtctc
     5161 cggtgcacag gccgaagagg tggtgttctg agacaatgtt ttcatgtatg aaatccttgt
     5221 aatgtgaata aatgagaggg agtaaaggtc gggagctggc aacatcacgt tcatcactga
     5281 ccgtgatgac ccggtaatat cagggatgaa tgatattcgg ctgaaattcc ggacagccgt
     5341 acgcgtaacc gttccggtca cagacggatg gccagatgcc attgcctgta tactcagtct
     5401 gcacagactg atttctccgg gacggtaaag cagacgggta caccaggcgc acaggtcatc
     5461 atcagaaatg gaacgacacc ccggcagcgc ctccggggcg tcgggtgatt ctggtgcctg
     5521 aatcacgcct tcagggacat gctgcatcag cgcaggctct ccagcagcgt ttctgccatc
     5581 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
     5641 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
     5701 gtctgataag tactccacag gtccttaccg taatcctccc ggcgtcgtgg cgtcagaatg
     5761 tcggcggtgg tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
     5821 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg tttctcctca
     5881 atccggtcaa acacgcccac cacttcgtaa gccccttcaa taactttctc caccacgttt
     5941 ccccggtgcg gaacacgcac ttcccccaga gactgaccac agacacaccc gttctggcag
     6001 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg ggagttgagc
     6061 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
     6121 tgttttgtgt atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaaaaagggc
     6181 tggaagcctt cccgctgcag actttccagt acggtgatgg tggggatgta cgtatagttc
     6241 tggcttcggg acgtgtgttt ctcttccccg aaaatccccg gcacatggtg catcagctct
     6301 tcatgtgtca gcggacggtc acggcgtatc tggttcgcat aaccaaaacg actgactaat
     6361 tgcatacttt gctccttatc ggtggttaag atttactggt gcaacaaatg aaaaaagccg
     6421 cgtctcccgg agaagacgcg gcctgatgga tgaaatcaat gaagtttatt gtctgagaag
     6481 ccctccactg gcgagccacg cattaagctg ttttccggca tcagcttcag agttccggct
     6541 gcgtcagcgt cgctgacctt ccgcgttgcg gataacccga cggaaatgca ttcccgggtc
     6601 atcctggata aacacattgc ggtaagcggt tatgacagca accgcctgcc cgtatgagaa
     6661 agatccttca gccaggacat actctgtgtg taacccggca tatctggttt ctcctgataa
     6721 atagcctctg ccatacgttg tggcagaggc tgaagcatga aactgacttc agggatcagt
     6781 taacattttt tccggaaacg gtaatcagca gtggatggta gtcctgggga tcgaaaaccg
     6841 ataacggcag actgacacga tggccgttac tttcttcagt tgctttaatg atttcggttg
     6901 tggcgacatt ttccacgcac tccgtttcca gaaatgcgtc tgtggttcgc gtggcattac
     6961 tgtcaccaaa agcttccgtt tccatttttc tggtcaccag cgtctgacca tatttgtctt
     7021 tgagttgcag agtgatggtg agggggccaa atccttcacc gtttccgcca ttatccagcc
     7081 ggaactggta agcacaaata tttcccggga gccatatcgt atctgtattg cgtatactga
     7141 tgtaacgttg atcctgtgcc cggagtgggg cagaccacgt taaccccaga atgaaggcgg
     7201 taatcatgca ggttttgaac aggtgaatca tggtatttac ctctctgagt catgacgatg
     7261 acactggcaa atcaggtgat aaaacctaaa aggagcagaa cacacgttat gccgggagct
     7321 ccggggggaa tgcttcttcc agccggttaa ccatcttgcc gagatgggat gcatcatatt
     7381 ccatgacggg gcgttgcctg atgatactga ccaccagtgg tttgattaac atattggtcg
     7441 cggcccgttg ttgtataccg gcggcgaaaa tgatctccag agcattctga tcgataacac
     7501 gggccagatt cgcgttcaga ttttcaatct catgtccggt cagcaggaaa ctgccggcaa
     7561 gccatgtcac ctgctcctga atcattgcca gaattttgac gcgatcttct tcaaccagcg
     7621 ctccggcaat ttttcctgat acagcggagg caatcattcc ccccagaaca ccgccaacca
     7681 cacgccctgt cagtgcacca aatggtccca gtggactgaa caatatcccg cccgccactg
     7741 acccgacagc accacctgcc acaccggaag atgccacggc aagattcctg atgaactgcg
     7801 caccggagat ccgtccccgc aacattttga tcatgtcagg gctggttgtg acagctacca
     7861 gtgccagaga tgtcaccagc gtacccttca tcactttgtt cagggcactg atatttcctg
     7921 caccggtccc cttctgaagc gcctgccgga cagtcgggct ggccgtcgaa aaatcaatat
     7981 gcttcagcat accctgaaca acagtgagcc ggtgaagttg ctgggttgtg acgtagacag
     8041 ccagtgtgcg ggtgaaggtt tttcctgcct ggacagcagc tgtctgcagg gcagcatcgc
     8101 gatcgccggt gctgagccag aagaccgatg ccgtcagggc aaaactgatc cctccggccg
     8161 ccagactgac aaccgacccc tcggcaatat cataagtgac cgattcgatg gtcccgaacc
     8221 gggtgatatt acgggcctgg gtataagtca gatgtccccg acgaatcagc cgggacgctt
     8281 ctgcgggatc ggttacaccg ggtactttac cttcgcggat cttattcttc atggtttcca
     8341 cagcgccggc atactgatca cgggggactt ccagttgcat gggaccatga tttcccatgt
     8401 aacgatactg tccgttttgt ccgtcgaatg ccgcaccgac gctacgggct gcagttgaac
     8461 aatatttcgt ctggatttct gttccgctga ccagtctgtc tgctccattt ctggcattat
     8521 tatcaccgag gatccgtgcg tttttcagac ggactctgtc gacaatatgg tttccccgtt
     8581 cagcggcaaa cccatgtccc tgccctccgg aagcaaacat tttctcattt gtactccagg
     8641 tttccggcag tttcatgaga atcagaccat tcgccgttgc atcggaaata aaaccggtgt
     8701 aagaacgttc ataaatgtcc tgacacagtg tttctgtttc gtgacaaaga gaggagcagt
     8761 accattccgg agccggctta tgattttccg gggcatgatg tacttctgtc agcccgcagg
     8821 aggcacagat acgaagctca tccacgtggt ggaatgatac aaaacggcgg ccttctgaaa
     8881 aggggtacca gtcaccgccg tccaggcaac gatcgagaaa atcgtcattc gcttccggtg
     8941 ataaattcag gcggttccgg aagagcgttt tcagttcatc aggtgtgaat gacaggttct
     9001 ctttcaactg ttcgttaaac cctgccatgt ttctttcata ccaggcattg ttcagtaacc
     9061 agtttccgtc tgccagttca aattccctca gttctccccg ggaaatccgt tgtgaaatga
     9121 gtgtcagcca gatgcgctcc gtcagcgttt caggaaggct gtaatcgttt tgcaaactgc
     9181 tcagccacga tttactgttg agcaccggtt gttcttccag tgtctggctg atggcactgg
     9241 tcagtctttt cagcgcctgg atctggctgg ccgcccatag cagcgtcact aatgcagcca
     9301 gccagtacaa caacccgccc caccacatca gtgagggtga aatatcgaac tgcaatgcga
     9361 tcaatggtgc gtatgcagaa cacgccataa ggaaaaaagc agtataaaaa cagcagtaat
     9421 acccgttaat ttccttgctg atatcttcag gtgtgagccc gttctgctga atggttctta
     9481 tctgcacggg aagagccagc agaccagcgg cgagagcggt ggtcagaata aattttacag
     9541 agtcaaaagt atgagcctcc agtgcgatat gtttaccatc aagtccccac atgaagctga
     9601 tgatcattcc ggcaatcgtc agaaatacgc ctgccagcca gcgaatatgg aataaccggg
     9661 ttatgcgttc agtgatatag ccagccagta aagccggacc gaacagacac agcaatatga
     9721 accctgccag cgcagacgct gtgctggtta atgctctgaa caaaaggcga agaatggcaa
     9781 atcctgcgat cagcagaatc agcgcgccga ctatctgtaa catagtcact ccgtgatgaa
     9841 tatcatgtta ttgtgaatgc cagtgaatgt ggcactgaaa cgttttcccc cgtccgggtc
     9901 ccggtcatca tgaccgggac cacagagagg caatgcagaa ttatcagaag gttacattca
     9961 gtgtggcctg actgttataa ccttcagcgc tgctgccgtt gatgctgtgg gcataactgg
    10021 cctgaacgcc cagggtgata ttttcccgga cacgggcttc cagtccggcc tgcaggtcca
    10081 gtgacgtacc attctgtgac ggtgagaacg tcatgttact gccggctgcg gctgtaccca
    10141 tgctcatgtc cccccgggag ctgaaggtgc ggataacaga aggctgtacc caccagttca
    10201 ccggcagttc acgcacactg tgtttggcac tgccgcgcag ggtgtcacgg gatgaggtgc
    10261 ctttaccaaa gttcatatcg tggtggctgc ccagacggaa gccggcacgc acatgttgtg
    10321 cactgccatg cccgaacttc acataactgg cgttatcctg gccgtcatcc agggagagtc
    10381 cctgccaggt gtactgcagt tgcggctcca gcatcagatt gtcagtgata ctgaagggca
    10441 gaccggtttc cagcgaaccc agccagcccc agccccggac gcggaagtcg ttattgtccg
    10501 atgaggcttt catgctgtgg cgggttccct gggccacaat gtcagcccac aggccggagg
    10561 cgttgtgtat cagattcagg tatccgccca ggctgccggc atcatcccgg accgtgccgg
    10621 cacgggagcc gtcatcatcc ttaacatcaa cggaagaatg gccagcagca ccatataccc
    10681 ccgcggtcac agacataccg gcaacctctg ttctgagcag gtcaccctcc agacggacga
    10741 agccatagct gccgctgctt tccggcgtgg ctccacgggc aataccgccg ttgttatcgt
    10801 gaccgagatg accgccctga atgctgagac ggacgctgtt attttcaccg cttacaccgg
    10861 tctgatggct gcgggagcct gccagaatcc ggtcatagtc cattgcctgt gtcagcatgg
    10921 aggcatacag ggggacttct gcacgataag cattttcact gcgcagatac cagctctcat
    10981 cactgtcccg gttgagggag tagttaaagg caccggcctg cagcttattc ccctggataa
    11041 aggccccttc ctccgtggtg gcaccgttaa tggcttccac cacctgaata cccttaccgc
    11101 tggtcgccag ccccgacgca ctgttgccgg cgttcaccag gttcaggatg gtttttccgg
    11161 ttgccctgcc accgtcaatg accagtctgt cagcattgtt ctgtgccata tccgggcgta
    11221 cacgcaggct gatggtgcca ttctgtccgt tcaggttttt cactttcagg gttgccggta
    11281 cgaacttccc tgtgcgggtg gaggtgaaat gaatctgtcc ggcatggctg aggtcatcca
    11341 ccaccgactg caccgtggcg ttatcgggaa tattccaggt ggcaccagag gcgagagtga
    11401 cattcgtggg gtcaatggca ccgttcagca cagtgctgcc ggtcagcttc agggctgtac
    11461 cgcgctgagc aatgacatcc gtggtgacgg tactgtcgtt cagcgtcagc gtgccttcat
    11521 tcaggttgac ggctttctgg gtgagtgtgg tgttgctgac agtgagtgtg ccacttcctg
    11581 atttttccac gctgccgtta ccggtgagag cgcctccctg caggagtgca tcgccttcac
    11641 ggatggtcag ggtatcgttg ttcaccgtct tcccggaaag ggtaagtatg gcgccgttat
    11701 tcagcgtggt ggtgcccgcc agtgtgccgc ccctggcggt gaacagtccg ccatttaccg
    11761 tggtatccgt ggccgtatca ccggcgttca gcgtgaatga actgcctttt tccagcatca
    11821 gggcatccgc ctgaccgcct ccgatactga atgcctttcc gtcgctccgg gtaccactga
    11881 cagcggcacc ggaatcggcc agcagtacac cgccatttcc catggatacg gtggtggcgg
    11941 tgccaccgtt gcggacatcc agcgttccgc catcatccac gcgggtatta gtggctgtgt
    12001 gtccggtcag cacatccagg cgtccgccat tttccagtac gacattatca gctttaccct
    12061 ccacaacaga gaatgctccc aggcggtttg tgccggtgac ggttgcagct gtactggtaa
    12121 ccagtgcacc gcccgtgttc tgggtgacat cagacgcttt accgccggca ttcacctgca
    12181 gctttccttt ctggttgatg gtggtatgcg cggcagttcc tccttcctta atcacctgcc
    12241 agccatcacg gtttatcagc gtctctgttg ccgtgccacc gttgtgtaca tactggttac
    12301 ccccctccag tcgggtgtta tgtgcctctc cgtgtaccgt ctggtcacca ccggcgtaaa
    12361 tgagggtgtc ccgtgccatc cccgaagacc agataacctg ccggccattt ttattgatgg
    12421 tggtggattc agccgtccct ccgaccatct gaccgctgga cacattttca gactccggac
    12481 caccttctgc accggtgttg acgatggttc cggttgccag tccgccatgt tttatggtct
    12541 ggtaaccatc ctggttaata attgtaccgg cagctttccc tcccccgtgt acccactgct
    12601 cacctctgtt atccagcgtg gtgttcaccg ccagtccgtt aaggctctgc cctccgccat
    12661 cacgaataac cgtatcactg gcagttcctc ctgcctgcac aatctgacga ccatttgcgg
    12721 tgacagtggt gtttctgcct gtgccacccg cttttatcca ttgcccgccg gtgttttcgt
    12781 cactgtccgg ccccagctca agcccggtac tgacagtcac gccatcagct gttccggata
    12841 caaactggtt gtcatggttt accagtgttc cgccattcac tgtttcaccc gggtgcacaa
    12901 cgatgtcagc agccagcacc gggagtgacg tgaccgcggc aagagacagt gcaaccgcca
    12961 caccgccacg tttaccctgt gcgcgggcca gttcggaggc aaccacgaaa gcgcccgtaa
    13021 tgtgattcca taccagcctg tagcaggtat tcagatgtcg tttcatcagc atttccttaa
    13081 tcagagtgag ggtgaataaa aaagccggta tccacagatg tggataccgg caggtaaagg
    13141 gttacagtgc cggaggtcca gctgacggaa gtttctgacg tgtggctctg ttgttccctg
    13201 aatctcatgt ctgtgtgtat ctgcgcacgt tgctaacagg agttattatt agcataacga
    13261 tcgatattaa cgatcggtaa aataggtatc gatcgtttta ttctattatc agtctattgc
    13321 acggacaaga gagacagtaa cgaaaacagc gcttttgtga gcagactcag aggcagggag
    13381 gacatcatcc acaacaggtt cagaagaaga tccagttcca taccgcgcgg gcaacagaga
    13441 ccacggtgtc acgaacagcg cgcagggccg tacggacaac agaggcaaca cagacgctct
    13501 ccgctgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggcc tgagagcgga
    13561 cagactccgt gcgcagctcg tcctgcagtc gggtcatcag ggggctggcg gcatggtcgg
    13621 gaagcgctgt catgagcgca ctgaccagcg tatccagttc ccagccggtg cgggccgata
    13681 cggcaacaac cggatgtacg ggccggaaca gacggaatac cgcctccgtt ttctcacgaa
    13741 tgttctgttc ctgtgcggga gaaggctgaa tacctgccat atcccattca tggcagggtt
    13801 ccgttttgtc agcctgcgtc accacaaaca gcacctgctg atgtccgcac tgcaggatgt
    13861 gtcgccagaa atactcatcc acagacaggg cccggtcatc ggctttaatc agccacagca
    13921 ccaggtccag ttcaggcaga atgtcacggt acagggcttc atactctgca tccctgtccc
    13981 ggctctcgcc caccccgggc aggtcagtga taaccatgct gtgaccgtga ccactcagac
    14041 ggaagcgctg cacttcccgg gtgccggcgt gaacatcact gaccggtgtg acctccccct
    14101 gaaacagtgc attacagagt gaagatttac cggccccgct tttacccata atgccaatca
    14161 cgggttcgtg actggtgagt ttgcgcagat gttccaggat gtgacgggaa agtgagtaag
    14221 gcagggagga gagcggtttt tcaattgcct caatggcatc agacggattc atacatgtat
    14281 tcccggtgaa aaaagacaaa accccgcgca ccggaaaggt ggcgggggat tcacggagat
    14341 aatatctgtc tgagattatt tagaatgtaa caggtaatga attacttcct tcgcttgtgt
    14401 tcaagatatt tctctgtaat cagcatttca aaaaactctc ctgttctcag accgtaaaca
    14461 gttctgagct catcaagcat ttctttcaca ttgcttctta tatgtatatt aatggccttt
    14521 ttaccatcac tccgatattt gtccttttgt tttttctgat gccaggcatg atttattcgt
    14581 gttctgaaca gttcaatggt atcaggcgct tctttccata aagcaaaggc gacaagagag
    14641 gcaagataat attctttcgt tgaagatggt atgaaccacc gggtcactgg gttatgtact
    14701 ggatgttttt catcctgttt ttgtttcagt cttgtccaga accactgaca catttcatca
    14761 tcgtcaggaa ggagccactt aaacttttga caagcgcttt tctttttaag ccacgcctgc
    14821 ctgagttcct cgaggtatat gctttttgca tttttattaa ttaaagaatg gcaggagtca
    14881 aaatatgaaa tgattacttt tattcgttcg ttatgggatg atggattatc cggaaggtta
    14941 ctttttttgt aagtgtttaa cgtttcatcg gtaactgttc gtattttcct gacttcaatt
    15001 ccgtttttta ttcctgatga attatggttt attttcggga attttgtatg gtcttccttc
    15061 gacagataaa tagtagtcca ggtaaagaaa gtcgcaagtt cgtcagtttt caaccagaga
    15121 aaactctctt caggcagtat tgcctgcccg gcaataattt caagctcatt aatttcgtta
    15181 ttgagtgctt cttcttcagt agtaccaaaa gtaccaaagg ggggaggtga aacattacaa
    15241 tacttttcct ttaaaaataa attaacattc ctgatacaat tttgtgggtt gtcaggcgca
    15301 aagtgaggga attcattagg gtatttgtga ttcagatatt cggcataaaa ccgacattta
    15361 cggggatctc tgcatttgat aagcatctgt tcgttattca ttatctcact ccatggtgcc
    15421 tctcttataa caaaatcgtc ttaattgtat ttaatcacta agtgattatc aaatgttttt
    15481 gttgctgttt atttatgttt ttaaatgttt atgtc
//
LOCUS       DAZSNG010000093        15412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZSNG010000093 DAZSNG010000000
VERSION     DAZSNG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.094"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1177)
                     /locus_tag="ACHW4Z_003057"
     CDS             complement(<1..1177)
                     /locus_tag="ACHW4Z_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8882603.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHW4Z_003058"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHW4Z_003058"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHW4Z_003059"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHW4Z_003059"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR8882604.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHW4Z_003060"
     CDS             2804..4306
                     /locus_tag="ACHW4Z_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR8882605.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHW4Z_003061"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHW4Z_003061"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8882606.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHW4Z_003062"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHW4Z_003062"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8882607.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHW4Z_003063"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHW4Z_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR8882608.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHW4Z_003064"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHW4Z_003064"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR8882609.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHW4Z_003065"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHW4Z_003065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR8882610.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHW4Z_003066"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHW4Z_003066"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR8882611.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHW4Z_003067"
     CDS             complement(11871..13067)
                     /locus_tag="ACHW4Z_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR8882612.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACHW4Z_003068"
     CDS             13260..13763
                     /locus_tag="ACHW4Z_003068"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8882613.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHW4Z_003069"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHW4Z_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR8882614.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACHW4Z_003070"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACHW4Z_003070"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR8882615.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DAZSNG010000094        15297 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZSNG010000094 DAZSNG010000000
VERSION     DAZSNG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15297
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.095"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..74)
                     /locus_tag="ACHW4Z_003071"
     CDS             complement(<1..74)
                     /locus_tag="ACHW4Z_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR8882616.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHW4Z_003072"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHW4Z_003072"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR8882617.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACHW4Z_003073"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACHW4Z_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR8882618.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHW4Z_003074"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHW4Z_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR8882619.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACHW4Z_003075"
     CDS             2704..2940
                     /locus_tag="ACHW4Z_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR8882620.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACHW4Z_003076"
     CDS             2943..3800
                     /locus_tag="ACHW4Z_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8882621.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACHW4Z_003077"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACHW4Z_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHW4Z_003078"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHW4Z_003078"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR8882622.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACHW4Z_003079"
     CDS             complement(6415..7311)
                     /locus_tag="ACHW4Z_003079"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR8882623.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACHW4Z_003080"
     CDS             complement(7360..7599)
                     /locus_tag="ACHW4Z_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR8882624.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACHW4Z_003081"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACHW4Z_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR8882625.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACHW4Z_003082"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACHW4Z_003082"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR8882626.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACHW4Z_003083"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACHW4Z_003083"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR8882627.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACHW4Z_003084"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACHW4Z_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR8882628.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACHW4Z_003085"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACHW4Z_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT         3512 a         4037 c         4025 g         3723 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtggg
//
LOCUS       DAZSNG010000095        15138 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZSNG010000095 DAZSNG010000000
VERSION     DAZSNG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.096"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHW4Z_003086"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHW4Z_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR8882629.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHW4Z_003087"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHW4Z_003087"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR8882630.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHW4Z_003088"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHW4Z_003088"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR8882631.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHW4Z_003089"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHW4Z_003089"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR8882632.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHW4Z_003090"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHW4Z_003090"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR8882633.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHW4Z_003091"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHW4Z_003091"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR8882634.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHW4Z_003092"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHW4Z_003092"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR8882635.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHW4Z_003093"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHW4Z_003093"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR8882636.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHW4Z_003094"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHW4Z_003094"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR8882637.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHW4Z_003095"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHW4Z_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR8882638.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHW4Z_003096"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHW4Z_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHW4Z_003097"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHW4Z_003097"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR8882639.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12258)
                     /locus_tag="ACHW4Z_003098"
     CDS             complement(11944..12258)
                     /locus_tag="ACHW4Z_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882640.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            complement(12300..12806)
                     /locus_tag="ACHW4Z_003099"
     CDS             complement(12300..12806)
                     /locus_tag="ACHW4Z_003099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882641.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13551)
                     /locus_tag="ACHW4Z_003100"
     CDS             complement(12952..13551)
                     /locus_tag="ACHW4Z_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882642.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="ACHW4Z_003101"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="ACHW4Z_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(14722..15096)
                     /locus_tag="ACHW4Z_003102"
     CDS             complement(14722..15096)
                     /locus_tag="ACHW4Z_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR8882643.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
BASE COUNT         4190 a         3663 c         3482 g         3803 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacaa tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggat
//
LOCUS       DAZSNG010000096        14469 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZSNG010000096 DAZSNG010000000
VERSION     DAZSNG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.097"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(12..953)
                     /gene="fabY"
                     /locus_tag="ACHW4Z_003103"
                     /gene_synonym="yiiD"
     CDS             complement(12..953)
                     /gene="fabY"
                     /locus_tag="ACHW4Z_003103"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR8882644.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(998..1435)
                     /gene="dtd"
                     /locus_tag="ACHW4Z_003104"
     CDS             complement(998..1435)
                     /gene="dtd"
                     /locus_tag="ACHW4Z_003104"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR8882645.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1432..2304)
                     /locus_tag="ACHW4Z_003105"
     CDS             complement(1432..2304)
                     /locus_tag="ACHW4Z_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR8882646.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2298..2897)
                     /gene="yihX"
                     /locus_tag="ACHW4Z_003106"
     CDS             complement(2298..2897)
                     /gene="yihX"
                     /locus_tag="ACHW4Z_003106"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR8882647.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2996..3781)
                     /locus_tag="ACHW4Z_003107"
     CDS             complement(2996..3781)
                     /locus_tag="ACHW4Z_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8882648.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3815..4711)
                     /gene="yihV"
                     /locus_tag="ACHW4Z_003108"
     CDS             complement(3815..4711)
                     /gene="yihV"
                     /locus_tag="ACHW4Z_003108"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR8882649.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4879..5775
                     /gene="yihU"
                     /locus_tag="ACHW4Z_003109"
     CDS             4879..5775
                     /gene="yihU"
                     /locus_tag="ACHW4Z_003109"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR8882650.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5799..6677
                     /gene="yihT"
                     /locus_tag="ACHW4Z_003110"
     CDS             5799..6677
                     /gene="yihT"
                     /locus_tag="ACHW4Z_003110"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR8882651.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6694..7935
                     /gene="yihS"
                     /locus_tag="ACHW4Z_003111"
     CDS             6694..7935
                     /gene="yihS"
                     /locus_tag="ACHW4Z_003111"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR8882652.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8073..8975
                     /locus_tag="ACHW4Z_003112"
     CDS             8073..8975
                     /locus_tag="ACHW4Z_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR8882653.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9174..11210
                     /gene="yihQ"
                     /locus_tag="ACHW4Z_003113"
     CDS             9174..11210
                     /gene="yihQ"
                     /locus_tag="ACHW4Z_003113"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR8882654.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11256..12630
                     /locus_tag="ACHW4Z_003114"
                     /pseudo
     CDS             11256..12630
                     /locus_tag="ACHW4Z_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12673..14076
                     /locus_tag="ACHW4Z_003115"
     CDS             12673..14076
                     /locus_tag="ACHW4Z_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882655.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14144..>14469
                     /locus_tag="ACHW4Z_003116"
     CDS             14144..>14469
                     /locus_tag="ACHW4Z_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR8882656.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3491 a         3769 c         3648 g         3561 t
ORIGIN      
        1 ctctccataa gctactcttc ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg
       61 ggcagaacga tatacgtgcc ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg
      121 acctgcatct gcacccgtgc ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag
      181 gcaccaaggt cggctaccgc atggggttta ccgctaatcg gtttgctgta gcggatatgc
      241 gcatccgcca gaataatcgt tccgccgagg tggcgttcac gcagcatcag ccagataagc
      301 ccccaaccgg tgagcgtcgc cagcgagaat aaactcccgg caaacagcgt atggtgcgga
      361 ttctgattgc cggtttccgg catggtggtg ataaattttt gcccggtata ttgttgaata
      421 cgcacgccca ttttttcact aagcgggata tgttcgtacc acgcctgttg cagctgcgcg
      481 caccagtcgc cgcgatgcaa aatatcatcc agagtggcga cgggcttaat catcaaaaaa
      541 tggcgaatcg gcgtggtggt tggcgtggtg atttctccct gattaacaaa ccccagcttg
      601 gcgaaaaact ccaccgcgtc ttcacgggcg ctacaggtca cgcgcttaac gccttcctga
      661 cgcgccaccg actccagggt catcgccatc agcgttccta accctttgtc ctgcacgtcc
      721 gggtgaacga ccataaagcg aatggacgct tcattgtcgg cattaatata caatcggcct
      781 accgccacca gattaccctg ctcgtcgacg accatctggt gatgcgccat cgcatcccac
      841 gcgtcgcgtt ccgaaccttt tggttgatgc aggggcttac gcaacatttc ccagcgaaag
      901 tgatagtaac gctctaactc ttcttctgtt tgtggaacct gaaggtgata catagcgata
      961 ctctctcttg tttcccgtga caaccctgga agctggctca tacctgcaac cagaatgtca
     1021 cggggccatc attgaccagc gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt
     1081 tcatctcttg ctggcggcag cgttcgacga aatagtcata taacgcctct gcgcgatccg
     1141 gtgatgcgcc tttggagaaa cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa
     1201 actgggaaac caccagtaca ctgccgcccg cctgttgcac gttgagattc atcttgcctt
     1261 cggcatcgct aaagatgcgg tagccgagca cacgctcgca cagacggttt gctttctgtt
     1321 cgtcgtcatc cttttcgaca cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca
     1381 cttctccctc cacggtgacg ctggcacggg ttacgcgttg aattaatgca atcatggttc
     1441 gtcgtcttct tcttgttcag ctgcttgttt tagtttgcgg tattccccga gagtgacagt
     1501 aatttccgcg ccaagcaaga cgatacacca tgtccagtag acccaaacaa agagaatggg
     1561 gatcaccgcc agcacaccgt aaatgagctg atatgacggg aacatggtga tataaagcgc
     1621 gaaacctttc tttcctgctt cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc
     1681 gcggttaggt acgcggatgg taggaacaat gctgtacagc aaccagaagg agatccacga
     1741 caacagcaac ggaaaaatac gcagcacgtt gtcgatgaca gtattgagat cgctcgccca
     1801 gcgcagggag agcaaatagg aactgatcgc cagactggcc cctgccagca gcggccccag
     1861 cgttaaaatc atccagtaca cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg
     1921 ccagatggta ttcaacgcgc tatcgatgga gtacatcaac aataacgcgg tgacgatcag
     1981 cccgcacgcc ccaacggcgg tcatcttgtt ggaattagca acaaattgtt cgatataccg
     2041 ctgaataaca tcgccagtag caggcagaaa gttggcaaaa ataaagtgac gtaactgaat
     2101 gctgacgtcg gaaaacatgg gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg
     2161 cactaatgag agcaacgaca cataggcaag gttacctgcc agggttgtca tgttgtcctc
     2221 atcaatacgt tgccagagta gttttagcca ggcccatagt ggacgggtac ggtgcctggc
     2281 tttgtcctga atggttttta gcataacacc ttcgcgaaat agtccgggat ggtggtttta
     2341 tctttcacca gaatactggt aatgcctagc tgattagctc cttctatatt atcggcgtta
     2401 tcgtcgaaaa agaccgtatc gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa
     2461 attcgtgctt caggtttgcg catccccaga tcttgcgaca gatagatatg gtcagcagca
     2521 tcacgaattt ccgggtattc ttccggccag aaggtggtat gcaggcggtt ggtattggaa
     2581 agcaccacca cgcgatgccc ctgctcacgc agtttatgca tgatggcgat cacttccggg
     2641 cgcagcgcaa caaacaccgc ctgccagccg tgagagaact gctcgtagct tagcggtaga
     2701 gccatctcat gacacagcgc ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc
     2761 tgatgaaacg cctcccccat atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt
     2821 aaatcgctcc aggctcccag cacacggtta aagtcgatat cgacaatcac attacctaaa
     2881 tcaaagatat agagcatttt tgcctccttt cgcgccacaa gaaattaact gtagcgggaa
     2941 aggatgggtt tggctatgtg gcaaggtatg aagccaggtt aacggtcaca aaaatctacg
     3001 cgtcttcctg gggaagaatg atttcaattt ggctgtcttg taagcgtgat tttagcgcag
     3061 cgggcggcag ctcgtcggta aagagcgcag tgacctgtgc cacgttacca atttcaaccg
     3121 ccgccgaagc atgatactta gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca
     3181 tcatcgtttt caccacgtta gcttcgttta catcaaactc catcaacgcg ccatcgctct
     3241 caatcgcccc aacgcttgtt accagataat cagcacgaaa atcagccaca aaggacgccg
     3301 ctgaagggcc aatgatcccg ctattatgag agcgcaacgt accgccgggc accatcactt
     3361 caaagcgcgg gttgtggtaa agaatatgcg ccacacgcag gctgttggtg attatccgca
     3421 aatgattatg gttaagtaac gcccgggcaa catgctcaac agtcgtacca atggtgataa
     3481 atattgttga accatcagga atatagtctg ccaccgcttc ggcaatcgct ttttttttct
     3541 cggtttgcga aacctcacgc tgctcgaacg ccgtattaac gacgctggaa gcccgacccg
     3601 cgccaccgtg atggcgcgta atcaggcctt gctcgcttaa tttacgaata tcccggcgga
     3661 ccgtctgcgt ggagacatcc agcagatttg ccagctcatc aatattcata tacccacgct
     3721 cggcgatcag catgaggagt tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca
     3781 tgaaaattcc tcgaaaaacc atcaccctgg cattttatac aaaaagtgac aaaaaagatc
     3841 gggtttgatc acagtcaggg atcccggctc gtccaccggg acgtgtgcat tttaacgccg
     3901 ctacaccgct ggcgaagcgg actgactccg ctaaatcccc acttgttgcc agcgccaccg
     3961 ccaaagcgcc gtgaaaaaca tctcccgcac ctgtggtatc taccacatca actttgaagg
     4021 ccggttgatg ctgacgccca ccattttcca gccagtcgca gcctgcgcta ccctgggtca
     4081 catagacatg accatttgtg agcgtttgtg cctgttttag cgcactggcc atctctttca
     4141 cgcccgttaa gcgcgccaga cccggttctg aaaaggccgc gtgatcgctt aatgccacca
     4201 gctcactgat atcctgcggc gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac
     4261 gggccagggt gaaggctttt ttagcgccgt cgtgccagcg tacatctgcc agcacaacat
     4321 cccactgaga gaaatcaatt tcctccaacc actctgcgtc aggcagcaga tccgggctgg
     4381 ggtagttaat gattatccgc tcgcctttgg tatccaccat gatggcggat tgcgaagatt
     4441 tcgcctggtt ataccgtttg gtgtaacggg tgttaacgcc ccaggattcc agctctgcca
     4501 gcaggctgtt gcccgcgtcg tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca
     4561 gccttgccgc cgcaaccgct gccgtcgccg ctggcccgcc accaacttcc gtataatttc
     4621 tcgccacgta tttaccgctc tccgtcggta acccttccac gtaatagatg cgatccatca
     4681 cggttatacc tacacaagca acacgaatca tggtcattcc ttaaacattt tgggatgcgt
     4741 tcattttaat ttcaccaatt gtttaaaaag tgacggctgt caattttttg acgataaatt
     4801 acaaattcga tcaaaaacag acaagcaaag catgtcataa atgacaaaaa gtgacatcac
     4861 tgtattcagg agagggttat ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg
     4921 ccaatggcga gcaatttatt gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc
     4981 gaagctgtgc ggcatctggt agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc
     5041 gctaaagatg ccgaatttat cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg
     5101 ttgttcggtg aaaacggcgt ttgcgaaggc ttatctaccg atgcgctggt cattgatatg
     5161 tccaccatcc atccgctgca aaccgataaa ttgattgccg atatgcaagc caaaggcttc
     5221 agcatgatgg atgttccggt aggccgtact tctgcaaatg ccattaccgg tactctgtta
     5281 ctgctggctg gcggcaccgc tgaacaagtt gaacgtgcca caccgatcct gatggcgatg
     5341 ggcagtgagt tgatcaacgc tggcggtccg ggcatgggga tccgcgttaa gctcatcaac
     5401 aactacatga gcatcgcgct caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc
     5461 ctgaatcttc ccttcgatgt tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt
     5521 cacttcacca cttcctggcc aaacaaagtc ctcagcggag atctttctcc cgccttcatg
     5581 atcgatcttg cccataagga tcttggcatc gcccttgatg tcgccaacca gctgcatgtg
     5641 ccaatgccgc tgggggccgc ctcacgggag gtttatagcc aggcgcgcgc agcgggtcgc
     5701 ggtcgccagg actggtccgc cattctggaa caggtccgtg tcagtgccgg gatgactgcc
     5761 aaagtaaaaa tgtaacgact ggataaagga ataaatgaat gaataagtac accatcaacg
     5821 acattacgcg cgcatcgggc ggttttgcca tgctggcggt cgatcagcgc gaagccatgc
     5881 gcatgatgtt tgccgcggct ggggcacccg ctccggtagc cgatagcgtt ttaactgatt
     5941 tcaaagttaa cgctgcaaag gccctctcgc cttatgcctc ggcgattctg gtagatcaac
     6001 aattctgcta ccgccaggtg gttgagcaaa acgcgattgc caaaagttgc gccatgattg
     6061 tcgccgccga tgagttcatt cctggcaacg gtattccggt cgatagcgtg gttattgacc
     6121 gcaaaatcaa tccgctacag atcaaacaag acggtggcaa agccttaaaa ctgctggtgc
     6181 tgtggcgtag tgatgaagat gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac
     6241 tgtgccactc acacggtctg gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg
     6301 gcgataaatt cgatcgcgaa caagcgatca tcgatgccgc caaagagctg ggcgacagtg
     6361 gcgctgacct ctacaaagtt gaaatgccgc tttatggcaa aggtccgcaa caagagcttc
     6421 tctgtgcttc acaacgtctg aatgaccata tcaatatgcc atgggtgatc ctctcttcca
     6481 gtgtcgacga aaaactgttc ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg
     6541 gattcctggc aggccgtgca gtctgggcat cggtcgtcgg tttaccagac aacgagctga
     6601 tgctccgtga cgtttgcgca ccgaaattac aacaacttgg cgatatcgtc gacgaaatga
     6661 tggctaaacg ccgctaagat gaggataaaa ggaatgaaat ggtttaacac cctaagccac
     6721 aaccgttggc tggaacagga aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg
     6781 ccgactggtt ttggctggtt aggcaataaa gggcaaatca aagaagagat gggcacccat
     6841 ctgtggatca cagctcgtat gttgcacgtt tattccgttg ctgcggcgat gggtcgacct
     6901 ggcgcttacg cgttggttga tcacggtatt aaagccatga acggcgcact gcgtgataaa
     6961 aaatatggcg gctggtatgc ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag
     7021 ggctatcaac acttctttgc tctgctgggt gccgccagcg ccgtcacaac gggtcacccg
     7081 gaagcgcgca agctgctcga ttacaccatt gagattatcg agaaatattt ctggagcgaa
     7141 gaagagcaga tgtgcctgga gtcctgggac gaagccttca gcaaaaccga agagtaccgc
     7201 ggcggcaatg ccaatatgca cgcggtggaa gctttcttga ttgtttatga cgtcactcac
     7261 gacaaaaaat ggctggatcg cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc
     7321 agaaataatc attatcgcgt taacgaacac ttcgataccc agtggaatcc gctgccggat
     7381 tacaacaaag ataacccggc gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg
     7441 atcgaatggg gccgtttaat gctgcacatc catgctgccc tggaagcccg ttgcgaacaa
     7501 ccaccagcat ggctgctaga agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc
     7561 tgggcacccg atggagcgga cgggattgtt tataccgttg actgggaagg aaaaccggtg
     7621 gtccgcgaac gtgtacgttg gcctatcgtc gaagcaatgg gtacggtcta cgcgctctac
     7681 accgtcactg gcgatcgcca gtatgaaacc tggtatcaaa catggtggga gtactgcatt
     7741 aagtacctga tggactatga aaatggttcc tggtggcagg agctggatgc ggacaataag
     7801 gtcatcacca aagtctggga cggcaaacag gatatttatc acctgctgca ttgcctggtg
     7861 atcccgcgta tcccgttagc gcccggcatg gctccagcgg ttgcagcggg tctgctggat
     7921 attaatgcga aataactgat ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc
     7981 tccccaaagg ggcgagggga ccgtccggtg cagtttttgg gtttgacatc agcctcacgg
     8041 gaggctgtat gtcgttaatt aaggtgcctt gtatgcaaat caccaatatg cattgcagtg
     8101 gacagaccgt ttcacttgcc gcgggggatt atcacgcgac cattgtgacc gtcggtgctg
     8161 gtctggctga attaaccttt cagggttgtc atttagtcat cccgcacaaa cccgaagaga
     8221 tgccgctggc gcatctgggt aaagtgctta ttccgtggcc gaaccggatc gccaacggct
     8281 gttaccgtta tcaggggcag gaatatcagt tacccattaa tgaacatagt tcaaaagccg
     8341 ctattcacgg gctgcttgcc tggcgagact ggcaaattag tgaattaacc accaccagcg
     8401 ttaccctgac agcttttctg ccgcccagtt acggctatcc gtttatgctg gcttcacaag
     8461 ttgtttattc gctcaacgca cacacgggtc tttccgttga gatcgcaagc cagaatatcg
     8521 gcactgtcgc agcgccttat ggcgtgggta ttcatcctta tttaacctgc aacttaacat
     8581 ctgttgatga atatctgttc cagcttccgg caaaccaggt ttatgccgtc gatgaacacg
     8641 taaatccgac aacgcttcac cacgttgatg agctggattt aaatttcacg caggcgaaaa
     8701 aaatagccgc gacaaaaatc gaccacacct ttaaaaccgc taatgatttg tgggaaatga
     8761 ccattaccca tccacagcag gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc
     8821 aggtttatag cggtgaaaaa ttacagcgtc agggactggc agttgaacct atgagttgcc
     8881 cgcctaacgc ctttaattca ggtatcgact tattattact ggaaccagga aaaccgcacc
     8941 gcctgttttt taatatttac ggacagagaa aataacttgt attgattagc agtcaaaatc
     9001 attgaagaac atttcactcg ctaaaggcaa cattatgtat cttcaattta ttaagttaaa
     9061 aacagctcag cttctgtaaa gtttttatat atcagtttta tgagaatcat tttacataag
     9121 cctgtaataa caggaaggca acaatctcgt tctcaactct atggagatta gttatggata
     9181 cgccacgtcc acagttatta gattttcaat ttcatcagaa taacgacagt tttaccctac
     9241 attttcaaca acgtcttatt ttaacccata acaaagataa tccttgttta tggattggct
     9301 caggtatagc ggatatcgat atgttccgcg gtaatttcag cattaaagat aaactacagg
     9361 agaaaattgc gcttaccgac gccatcgtca gccagtcacc ggatggttgg ttaattcatt
     9421 tcagccgtgg ttctgacatt agcgccacgc tgaatatctc tgccgacgat cagggccgtt
     9481 tattgctgga actacaaaac gacaacctta accacaaccg tatctggctg cgccttgccg
     9541 ctcaaccaga ggaccatatc tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg
     9601 gcaaaccgtt cccgctatgg accagtgaac aaggcgttgg tcgcaacaaa caaacctatg
     9661 tcacctggca ggccgactgc aaagaaaatg cgggcggcga ctattactgg actttcttcc
     9721 cacagcctac gtttgtcagc acgcagaagt attactgcca tgttgataac agttgctaca
     9781 tgaacttcga ctttagcgcc ccggaatacc atgaactggc gctgtgggaa gacaaagcta
     9841 cgctacgttt tgaatgtgct gacacataca tctccctgct ggaaaaatta actgccctgc
     9901 tgggacgcca gccagaactg cccgactgga tttatgacgg ggtaacgctc ggcattcagg
     9961 gcgggacgga agtgtgccag aagaaactgg acaccatgcg taacgcgggc gtgaaggtta
    10021 acggcatctg ggcgcaggac tggtctggga tccgtataac ctcttttggc aagcgcgtga
    10081 tgtggaactg gaagtggaac agcgaaaact acccgcaact ggattcacgc attaagcagt
    10141 ggtatcagga gggcgtgcag ttcctggcct atatcaaccc gtatgttgcc agcgataaag
    10201 atctctgcga agaagcggca caacacggct atctagcaaa agatgcctct ggcggtgact
    10261 atctggtgga gtttggcgag ttttacggcg gcgttgtcga tctcactaat ccagaagcct
    10321 acgcctggtt caaggaagtg atcaaaaaga acatgattga actcggctgc ggcggctgga
    10381 tggctgactt cggcgagtat ctgcccaccg acacgtacct gcataacggc gtcagtgccg
    10441 aaattatgca taacgcctgg cctgcgctgt gggcgaagtg taactacgaa gcccttgaag
    10501 aaacgggcaa gctcggcgag atcctcttct ttatgcgcgc cggttctacc ggtagccaga
    10561 aatactccac catgatgtgg gcgggcgacc agaacgtgga ctggagtctc gacgatggcc
    10621 tggcgtccgt agtcccggcg gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca
    10681 gcgacattgg cggttacacc accctgtttg agatgaagcg cagcaaagag ctgctgctgc
    10741 gctggtgcga tttcagcgcc ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg
    10801 gcgacaactg gcagtttgac ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta
    10861 ccgttttcac caccctgaaa ccttatctga aagaggccgt cgcgctgaat gcgaagtccg
    10921 gtctgccggt catgcgcccg ctgttcctgc attacgaaga cgatgcacat acctattccc
    10981 tgaaatatca gtacctgtta ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc
    11041 gcagcgactg gacgctctat ctgccggagg ataactgggt acatgcctgg acgggtgaag
    11101 cgttccgggg cggggaagtt accgttaatg cgcccatcgg caagccgccg gtcttttatc
    11161 gcgccgatag cgaatgggcg gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc
    11221 ccgtggggaa actcatgggc aaagggagaa catcgatgag tcacatcaca acggaagatc
    11281 cagcaacttt acgcctgccc tttaaagaga aactctctta cggtattggc gacctggcct
    11341 ctaacatcct gctggatatc ggtacgcttt atcttttgaa gttttatacc gacgttctgg
    11401 ggctgccagg cacctatggc ggcattatct ttttgatttc aaaattcttt actgcgttta
    11461 ccgatatggg taccggcatt atgttggatt cccgacgcaa gatcggtcca aaaggtaagt
    11521 tccgtccttt tattctgtat gcgtcattcc cggtcacctt actggcgatc gccaactttg
    11581 tcggcacacc gtttgatgtc accggtaaaa cggtgatggc cactattctg tttatgcttt
    11641 acggactgtt tttcagcatg atgaactgct cctacggcgc aatggtcccg gcaatcacca
    11701 aaaaccccaa cgaacgcgcc tcactggcgg catggcgtca gggcggcgct acgctgggcc
    11761 tgctgctgtg cacggtggga ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac
    11821 ttggctatat cttcgccgcc acgctgtttt cactgttcgg cctgctgttt atgtggatct
    11881 gctactcagg cgtgaaagag cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc
    11941 ctggcctgtt gcaatctttc cgcgcaattg ccggtaaccg cccactgttc attctgtgta
    12001 ttgccaacct ctgcacctta ggggcgttta acgtcaagct cgccattcag gtctattaca
    12061 cccagtacgt acttaacgat cccatcctgt tgtcgtatat gggatttttc agcatgggct
    12121 gtattttcat cggcgtgttc ctgatgcctg gcgcagtcag acgttttggt aagaagaagg
    12181 tctatatcgg cggcctgctg atttgggtgc tgggcgatct gctcaactat ttcttcggcg
    12241 gcggctcggt cagctttgtg gcgttctcct gcctggcgtt cttcggctca gcgcctgaac
    12301 tgggcgctgg tttccgacac cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa
    12361 ggcacggtct acaccggctt caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc
    12421 ttccccggct ggatgctgac gcaaattggc tatgtgccga acgtcgcaca ggctgaccac
    12481 accattgaag ggttacgcca gttgatcttc atctacccaa gcgcactggc ggtagtcacc
    12541 attgtggcga tgggttgctt ctacagcctg aacgagaaga tgtatgtccg cattgtggaa
    12601 gagatagaag cccgtaaacg cacggcgtaa taataataaa cgacgccctg cggggcgtta
    12661 taaggagtga ttatgtctga ccataatcca ctgacattaa aactgaatct gcgggaaaaa
    12721 atcgcctatg gtatgggcga cgtcggttcg aatttaatgc tctgcatcgg tactctgtat
    12781 ctcctcaaac tttataccga tgaattaggg atgcctgctt actatggcgg gattatcttt
    12841 ctggtcgcga agttttttac cgcgtttacc gatatgctca ccggattttt actcgactcg
    12901 cggaaaaata ttgggcctaa aggcaaattt cgtcccttta ttttatatgc agcagttccg
    12961 gcggcgttaa ttgcaacgct ccagtttatc gccaccacct tttgtttgcc ggttaaaacg
    13021 acgattgcca ccacgctgtt tatgatgttt ggactttcat acagtctgat gaactgctcg
    13081 tatggtgcga tgatcccggc aattaccaaa aacccgaatg agcgcgcgca gctcgcggct
    13141 taccgccagg gtggtgcaac catagggcta ttgatttgta ccgtggcgtt tattccgttg
    13201 cagtcgcttt tttctgactc aaccgtcggt tatgcctgtg cggcacttat gttctccatt
    13261 ggcggcttta tttttatgat gctgtgctac agaggcgtca aagagcatta tgtggacaca
    13321 gcgccaaccg gacataaagc cagtattctc aaatcttttt gcgcgatatt tcgtaatcca
    13381 ccattgctgg ttttatgcat tgctaacctg tgtactctgg cggcatttaa tatcaaactg
    13441 gcgattcagg tctattacac ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg
    13501 ggattcttca gtatgggatg catcctcgtc ggcgtattac tggtgccagt aactgtaaaa
    13561 tgttttggta aaaaacaggt ttatttagct ggcatggtgc tatgggcggt gggtgatata
    13621 ctgaattatt tctggggaag taactctttc actttcgtca tgttctcttg tgtcgccttt
    13681 tttggcacgg cctttgtaaa cagcctgaac tgggcactgg taccagatac cgtagattac
    13741 ggtgaatgga aaaccggtat tcgtgccgaa ggttctgttt ataccggtta taccttcttt
    13801 cgtaaaattt ctgccgcact tgctggcttc ttgccaggca ttatgctgac gcaaattggt
    13861 tatgttccca acatcgcgca aagcgatgcg acattacagg gtttgcgtca gctcattttt
    13921 atctggcctt gtgcactggc aattattgct gcattgacga tgggattctt ttacacactc
    13981 aatgaaaaac ggtttgcatt gattattgag gaaatcaacc aacgcaaaaa taaagagatg
    14041 gcgacagaaa aaaaaacggc ttccgtaacg ttataaatgt caatagccgc tatttccatc
    14101 ctggtgggtg gcggcctccc tacgtttaaa aaatggactt attatgaaaa agattaatgc
    14161 aataatttta ttatcttctc tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa
    14221 cagggaggca tataatcttg cctccgatca gggcgaggtt atgctacgtg tgggttataa
    14281 cttcgatatg ggcgcgggta ttatgttaac caatacctac acctttcagc gagaagatga
    14341 actaaaacat ggctataatg aaattgaagg ttggtatcct ttattcaaac caaccgataa
    14401 attaaccatc cagcccggtg gcttaattaa tgataagagt atcagttcgg gtggtgcagt
    14461 gtatctgga
//
LOCUS       DAZSNG010000097        14316 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZSNG010000097 DAZSNG010000000
VERSION     DAZSNG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.098"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..3051
                     /locus_tag="ACHW4Z_003117"
                     /pseudo
     CDS             1..3051
                     /locus_tag="ACHW4Z_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="ACHW4Z_003118"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="ACHW4Z_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="ACHW4Z_003119"
     CDS             6768..7175
                     /locus_tag="ACHW4Z_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882657.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="ACHW4Z_003120"
     CDS             7717..8805
                     /locus_tag="ACHW4Z_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR8882658.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="ACHW4Z_003121"
     CDS             8816..11095
                     /locus_tag="ACHW4Z_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR8882659.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="ACHW4Z_003122"
     CDS             11088..12224
                     /locus_tag="ACHW4Z_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR8882660.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="ACHW4Z_003123"
     CDS             12221..14224
                     /locus_tag="ACHW4Z_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882661.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3754 c         3655 g         3361 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcac
//
LOCUS       DAZSNG010000098        14110 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZSNG010000098 DAZSNG010000000
VERSION     DAZSNG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.099"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..415
                     /locus_tag="ACHW4Z_003124"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHW4Z_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHW4Z_003125"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHW4Z_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR8882662.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHW4Z_003126"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHW4Z_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHW4Z_003127"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHW4Z_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHW4Z_003128"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHW4Z_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHW4Z_003129"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHW4Z_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHW4Z_003130"
     CDS             complement(7513..8094)
                     /locus_tag="ACHW4Z_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882663.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHW4Z_003131"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHW4Z_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR8882664.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHW4Z_003132"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHW4Z_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHW4Z_003133"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHW4Z_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHW4Z_003134"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHW4Z_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8882665.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3458 g         3794 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagata ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZSNG010000099        13249 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZSNG010000099 DAZSNG010000000
VERSION     DAZSNG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.100"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHW4Z_003135"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHW4Z_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHW4Z_003136"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHW4Z_003136"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR8882666.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHW4Z_003137"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHW4Z_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHW4Z_003138"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHW4Z_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR8882667.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHW4Z_003139"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHW4Z_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR8882668.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHW4Z_003140"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHW4Z_003140"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR8882669.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHW4Z_003141"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHW4Z_003141"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR8882670.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHW4Z_003142"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHW4Z_003142"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR8882671.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHW4Z_003143"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHW4Z_003143"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR8882672.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACHW4Z_003144"
     CDS             9656..10102
                     /locus_tag="ACHW4Z_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR8882673.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACHW4Z_003145"
     CDS             10113..11564
                     /locus_tag="ACHW4Z_003145"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR8882674.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACHW4Z_003146"
     CDS             11554..12624
                     /locus_tag="ACHW4Z_003146"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8882675.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13249
                     /locus_tag="ACHW4Z_003147"
     CDS             12624..>13249
                     /locus_tag="ACHW4Z_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR8882676.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3159 a         3458 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaata
//
LOCUS       DAZSNG010000100        13098 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZSNG010000100 DAZSNG010000000
VERSION     DAZSNG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.101"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHW4Z_003148"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHW4Z_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR8882677.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHW4Z_003149"
     CDS             1131..1931
                     /locus_tag="ACHW4Z_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8882678.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHW4Z_003150"
     CDS             2009..2779
                     /locus_tag="ACHW4Z_003150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8882679.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHW4Z_003151"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHW4Z_003151"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR8882680.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHW4Z_003152"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHW4Z_003152"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR8882681.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHW4Z_003153"
     CDS             5034..5756
                     /locus_tag="ACHW4Z_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8882682.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHW4Z_003154"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHW4Z_003154"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR8882683.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHW4Z_003155"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHW4Z_003155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR8882684.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHW4Z_003156"
     tRNA            7149..7225
                     /locus_tag="ACHW4Z_003156"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHW4Z_003157"
     CDS             complement(7356..8402)
                     /locus_tag="ACHW4Z_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR8882685.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHW4Z_003158"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHW4Z_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHW4Z_003159"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHW4Z_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR8882686.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHW4Z_003160"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHW4Z_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR8882687.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHW4Z_003161"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHW4Z_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHW4Z_003162"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHW4Z_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3392 c         3484 g         3215 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc tagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZSNG010000101        13017 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZSNG010000101 DAZSNG010000000
VERSION     DAZSNG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.102"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..319)
                     /locus_tag="ACHW4Z_003163"
     CDS             complement(<1..319)
                     /locus_tag="ACHW4Z_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR8882688.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="ACHW4Z_003164"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="ACHW4Z_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHW4Z_003165"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHW4Z_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR8882689.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHW4Z_003166"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHW4Z_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR8882690.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="ACHW4Z_003167"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="ACHW4Z_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR8882691.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="ACHW4Z_003168"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="ACHW4Z_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR8882692.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHW4Z_003169"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHW4Z_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR8882693.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHW4Z_003170"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHW4Z_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="ACHW4Z_003171"
     CDS             10613..11503
                     /locus_tag="ACHW4Z_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR8882694.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="ACHW4Z_003172"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="ACHW4Z_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR8882695.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="ACHW4Z_003173"
     tRNA            complement(12493..12569)
                     /locus_tag="ACHW4Z_003173"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13017
                     /locus_tag="ACHW4Z_003174"
     CDS             12812..>13017
                     /locus_tag="ACHW4Z_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR8882696.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3188 a         3376 c         3304 g         3149 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctagtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctgaa
//
LOCUS       DAZSNG010000102        12980 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZSNG010000102 DAZSNG010000000
VERSION     DAZSNG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.103"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(5..1066)
                     /locus_tag="ACHW4Z_003175"
     CDS             complement(5..1066)
                     /locus_tag="ACHW4Z_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8882697.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1279..2742
                     /gene="bepA"
                     /locus_tag="ACHW4Z_003176"
     CDS             1279..2742
                     /gene="bepA"
                     /locus_tag="ACHW4Z_003176"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR8882698.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            2763..3122
                     /gene="arsC"
                     /locus_tag="ACHW4Z_003177"
     CDS             2763..3122
                     /gene="arsC"
                     /locus_tag="ACHW4Z_003177"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR8882699.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3255..3956)
                     /locus_tag="ACHW4Z_003178"
     CDS             complement(3255..3956)
                     /locus_tag="ACHW4Z_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR8882700.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="ACHW4Z_003179"
     CDS             complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="ACHW4Z_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR8882701.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5426..6052)
                     /gene="upp"
                     /locus_tag="ACHW4Z_003180"
     CDS             complement(5426..6052)
                     /gene="upp"
                     /locus_tag="ACHW4Z_003180"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR8882702.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6376..7413
                     /gene="purM"
                     /locus_tag="ACHW4Z_003181"
     CDS             6376..7413
                     /gene="purM"
                     /locus_tag="ACHW4Z_003181"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR8882703.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7413..8051
                     /gene="purN"
                     /locus_tag="ACHW4Z_003182"
     CDS             7413..8051
                     /gene="purN"
                     /locus_tag="ACHW4Z_003182"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR8882704.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8223..10289
                     /gene="ppk1"
                     /locus_tag="ACHW4Z_003183"
     CDS             8223..10289
                     /gene="ppk1"
                     /locus_tag="ACHW4Z_003183"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR8882705.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10294..11835
                     /gene="ppx"
                     /locus_tag="ACHW4Z_003184"
     CDS             10294..11835
                     /gene="ppx"
                     /locus_tag="ACHW4Z_003184"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR8882706.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11874..>12980)
                     /gene="pdeF"
                     /locus_tag="ACHW4Z_003185"
     CDS             complement(11874..>12980)
                     /gene="pdeF"
                     /locus_tag="ACHW4Z_003185"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8882707.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3356 a         3368 c         3271 g         2985 t
ORIGIN      
        1 aagattattc ttgcgcgatt tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg
       61 tcgccaatgg aatggcgaag aatacgcccc agaatcccca caaaccaccg aagatcacca
      121 ccgataaaat aatcaccagc ggatgcaggt taaccgcttc ggagaacaac accggtacta
      181 gcaggttgcc gtccagcgcc tgaataatca gatacaccgc gaagcagctc cagaattcag
      241 tacctgcgcc aaactggaat agcgccacgc caaccaccgg aatggtcacc acaaatgcgc
      301 caatgtacgg aataagaacc gagaagccga ccagcactgc cagcagcagc gaatagttca
      361 gcccgaagag caagaacccc agccaggtgg cgatccccac cacgatcatc tccagcactt
      421 tgccgcggat atagttggtg atttgttgat tcatctcctt ccacacctgt cctgccagcc
      481 cacggttacg cggcagcacc cggcgaacgg cgttcagcat ctgctctttg tctttcagca
      541 ggaagaagac catcaatggc accagcacca gatagacggc tatggtcagc aaaccgacca
      601 gcgaggcgag ggaaattttc accaccgaat cgcccatggt cagcatccga ctgcgcatat
      661 tttcggccat tgcatcaata atgcccgcgt ccattaacac cggatagcgg cgcggcaacg
      721 tggcggcaaa gtcagaaagc ttattgagca tccccggcat atcgcggatt aagtagatgc
      781 cctgttgcca ggcgataggc agtaccacga acgccatcag tagcaatata ccgacaaaaa
      841 ccaccaatac aatcgacgtc gcccagcggc gggagcagcc aatagattgc aggcgcacgg
      901 ttggccattc cagcaaataa gccagcacaa tagccaccag caacggagca agcaggccac
      961 taaagaaaaa gataatgcca aatccggcaa ctaaaataac cagcaaggca atcgcttccg
     1021 ggtcgctaaa acggcggcga taccattgca tcaacatttc gagcataaag accttccctg
     1081 aacctcaaga gcgggattgc gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc
     1141 tttttatgac ggattcagga aactgaaaag tcatttgagt gggctaatct tcgccgttac
     1201 actcaaaggc ggcgaggtgg gaacgatatt tcacagtatc ggtcaaatga ctatcttcag
     1261 aaatacagga tagaggttat gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt
     1321 gctgctatga ccattggtca ggtagccccg gcgtttgccg acagcgcaga caccttgccg
     1381 gatatgggaa cctccgcagg aagcacgctt tccattggtc aggaaatgca gatgggcgac
     1441 tattatgtcc gccagctacg cggcagcgcg ccgttaatta atgacccgct gttaacgcaa
     1501 tatattaatt cgctggggat gcgtctggtt tcgcatgcca attcggttaa gacaccgttt
     1561 catttttttc tgatcaacaa cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg
     1621 gtgctgcact ctgccctgtt ccgttattcc gataacgaaa gtcaactggc ttcagttatg
     1681 gcgcacgaaa tctcccacgt cacccaacgt cacctggcgc gagcgatgga agatcaacaa
     1741 cgcagcgcgc cgctgacctg ggtcggcgcg ttaggttcta ttttactggc gatggccagt
     1801 ccgcaggcgg ggatggcggc gctgaccggt acactggcgg gaacgcgcca ggggatgatc
     1861 agtttcaccc agcaaaatga acaggaagcg gaccgcattg gtattcaggt gctgcaacgc
     1921 tcgggattcg atccacaggc gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt
     1981 tactcctcgc gcccgccgga aattttattg actcacccgt tgccggaaag tcgtctggca
     2041 gatgcccgca accgtgctaa tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc
     2101 tatctggcga aagcgcgcac actggggatg tataattccg gacgtaacca gctcaccagt
     2161 gatttgctgg atgaatgggc gaaaggaaac gttcgtcagc aacgagcggc gcaatatggt
     2221 cgtgctttac aggcgatgga agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg
     2281 ttactggcgg cagaacctgg caacgcatgg tatctcgatc tggctaccga tatcgatctt
     2341 gggcaaaaca aagccaatga ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc
     2401 aatccggtgt tgcagctcaa cctggcgaac gcttatctgc aaggcggtca accacaagaa
     2461 gcggccaata ttctgaatcg ctacaccttt aataataaag atgacagcaa cggctgggat
     2521 ttgctggcac aggcggaagc cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca
     2581 gaaggttatg cgctcgccgg acgactcgat caggccattt cgctgttgag tagcgccagt
     2641 tcgcaggtga aattaggcag cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg
     2701 cgccagctgc agaaacgctt taagccttat accaagatgt aattcgcatt aaaggaagaa
     2761 caatgaccaa acaggtaaaa atctaccata acccacgctg ttcaaagagc cgggaaacgc
     2821 tgaacctgct gaaagaaaac ggcgtggagc cagaagtggt gctctacctt gagacacccg
     2881 ccgatgcggc aacgctgcgc gatttgctga aaatgctggg gatgaatagc gcccgtgaat
     2941 tgatgcgcca gaaagaggat ctttataaag aactaaacct ggctgacagt tcgcttagcg
     3001 aagaggcgct gattcaggca atggtggata atccgaagct gatggagcgc ccgattatgg
     3061 tcgcgaatgg caaagcgcgg attggtcgtc caccggagca ggtactggaa atcgtgggtt
     3121 aagatgtgct ccatcgctag ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat
     3181 ccggcctacg aatactatgg attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac
     3241 aataaacacc ttatctacaa cttcagaatt tctttcacaa acggaatggt cagcttacgt
     3301 tgcgcggtaa tcgacgcacg atccaactga tccaacgtca taaatagcgt gcgcatttct
     3361 ctgtcgagcc gcttcagcaa gaaacgcccc acatcttccg gcagttcaaa accacgcaaa
     3421 cgcgcgcgta actgtagcgc ctgcaactta tcttcatcag aaagtggctg caatttgtag
     3481 atctgccccc agtcgagacg cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt
     3541 ggacgatcgc cggtgatcaa caaccgtgtt ttgcccgatt ccagaattcg attgtagaga
     3601 tcgaaaatcg ccatctccca caactcatcg cctgcaatac actcaatgtt gtcgatacag
     3661 accagcgaca aatgctccat accgtcgagc acttccggaa caaaccaggt gcgtttatcc
     3721 agcgggacat agcccaccgc atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc
     3781 agatggctgc gccccgcgcc ttcgcgtgcc cagagataga tgtaaccgct atgttcctga
     3841 cgcagcacgt tttgcagcgc ggccagtaaa gaggagttat cccccggcca gaaacttgca
     3901 aaggtttcgt cgtcaggaag ataaagtggc aaagagagct gtgccggtgt gttcagagat
     3961 acctcaacca ggatttcaca aaatcgcgag aagtttacca cagaatccca tgatgtttga
     4021 accgggcagc aacactgccc ggtcggtaca ttatttgtct gttatatccg cgtcttctgc
     4081 gtccagcacc acttcttcag ggcgcagcac actgatcagt ttgaaaatca aacttaagcc
     4141 gataccgacg atggtcgcca gcgccatacc ttttaactcc gccgcgccga tgtttacctt
     4201 cgcgccactg acgccgatga tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt
     4261 gtaatccact ttcgattcga tcaaaacacg aataccggaa gcaccaatga caccataaag
     4321 cagcagcgaa acgccgccca tcaccggcaa tgggatcatc tggatagcgg cagccagttt
     4381 accgacgcag gaaagcagga tagcgaaaat cgccgccccg ccgataaccc aggtactgta
     4441 aacacgggtg atcgccatca cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc
     4501 aaagaagccg gagatcacgg tcgacaggcc gttagcaaac atcgaacggt gcagacctgg
     4561 atcgcgcagc agatcttttt tgacgatatt agccgttact accaggtgcc ctacgtgttc
     4621 ggcaataacc actaacgccg ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg
     4681 cgtatagagg gttggcagtg caaaccagtg agcattaata atcggcgtgg tatcgacaat
     4741 tcccattgcg aaagagagcg cgtaccccac cagcacgcca attaaaatcg ggataattgc
     4801 caggaaacca cgaaacagca cggaacctaa aaccgtgacc gccagggtgg taatagagat
     4861 gatgatggtt ttggagtttg gcgtttgccc ttcagccggg agtaaacccg ccataccggc
     4921 agctacgccc gccagctcca gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg
     4981 aaacagcacg tccaaccagc cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa
     5041 cagcacgccg cacataataa agccgcccag cgcgacttca taccctaacg gcaacagtaa
     5101 caataccggt gaaataaagg caaagctgga accaagataa gccggaattt tccctttaca
     5161 gatgaagaga tacagcagcg ttccaatacc gttaaataac agtacagtcg ccgggttaat
     5221 atgaaataag acgggcacca ggacggttgc accaaacatg gcgaacaaat gttgcaaact
     5281 aagcgggatt gtctgtaaaa gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat
     5341 agtattatcc tctgtattat gtgttatagg cgctttactc aaaaaaaagc cgactcttaa
     5401 agtcggcttt aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc
     5461 cgaggcccgg aataatgtat ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca
     5521 gttcgacgtc cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca
     5581 ccagaacttt gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg
     5641 aaccaccggt tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa
     5701 ccagtttctg gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa
     5761 cgctgatgcg cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac
     5821 gcagaattgg cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt
     5881 tccagccttc gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca
     5941 ggctacccac ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca
     6001 tcagtcccag cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc
     6061 tcctttgagg ggcagccaca aaaaaatcga cggattatac ctcctttctt cagggcggca
     6121 atattatttt cgttgacttt agtcaaaatg ataacagttt gagataaagt tattttatat
     6181 tcaaatggtt atgaaagaag attattccat ccgaaaacta acctttgccc tggcacaagt
     6241 cttctttcgc cgcgcgcccg gggaaaagac gtgcaaaaag gttgtgtaaa acagtctcgc
     6301 aaacgtttgc tttccctgtt agaattgcgc cgaattttat ttttctaccg caagtaacgc
     6361 gtggggaccc aagcagtgac cgataaaacc tctcttagct acaaagatgc cggtgttgat
     6421 attgacgcgg gtaatgctct ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt
     6481 ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat
     6541 cgtgaacccg tgctggtttc cggcactgac ggcgtaggta ccaagctgcg tctggcaatg
     6601 gacttaaaac gtcacgacac cattggtatt gatctggtcg ccatgtgcgt taatgacctg
     6661 gtggtgcaag gtgcggaacc gctgtttttc ctcgactatt acgcaaccgg aaaactggat
     6721 gttgataccg cttcagcggt gatcagcggc atcgcggaag gttgtctgca atcgggctgt
     6781 tctctggtgg gtggcgaaac ggcagaaatg ccggggatgt atcacggtga agattacgat
     6841 gtcgcgggtt tctgcgtggg cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc
     6901 agcgacggcg atgtgctgat tgcactcggt tccagcggtc cgcactcgaa cggctattcg
     6961 ctggtgcgca aaattcttga agtcagcggt tgtgatccgc aaaccaccga acttgatggt
     7021 aagccattag ccgatcatct gctggcaccg acccgcattt acgtgaagtc agtgctggag
     7081 ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga ccggcggcgg cttctgggaa
     7141 aacattccgc gcgtattgcc agataatacc caggcagtga ttgatgaatc ttcctggcag
     7201 tggccggaag tgttcaactg gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat
     7261 cgcaccttca actgcggcgt cgggatgatt atcgccctgc ctgctccgga agtggacaaa
     7321 gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga aaatcggtat catcaaagcc
     7381 tctgattccg aacaacgcgt ggttatcgaa taatgaatat tgtggtgctt atttccggca
     7441 acggaagtaa tttacaggca attattgacg cctgtaaaac caacaaaatt aaaggcaccg
     7501 tacgggcagt tttcagcaat aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg
     7561 gtattgcaac gcatacgctc atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg
     7621 agttgattca tgaaatcgac atgtacgcac ccgatgtggt cgtgctggct ggttttatgc
     7681 gcattctcag cccggcgttt gtctcccact atgccgggcg tttgctgaac attcaccctt
     7741 ctctgctgcc gaaatatccc ggattacaca cccatcgtca ggcgctggaa aatggcgatg
     7801 aagagcacgg tacatcggtg catttcgtca ccgatgaact ggacggtggc ccggttattt
     7861 tacaggcgaa agtcccggta tttgctggtg atacggaaga tgacatcacc gcccgcgtgc
     7921 aaacccagga acacgctatt tatccactgg tgattagctg gtttgccgat ggtcgtctga
     7981 aaatgcacga aaacgccgcg tggctggatg gtcaacgtct gccgccgcag ggctacgctg
     8041 ccgacgagta atgcccccgt agttaaagcg ccagctctgc cgctggcgtt tttcaattca
     8101 cctgtaaatc gcaagctcca gcagtttttt tccccctttt ctggcatagt tggacatctg
     8161 ccaatattgc tcgccataat atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg
     8221 taatgggtca ggaaaagcta tacatcgaaa aagagctcag ttggttatcg ttcaatgaac
     8281 gcgtgcttca ggaagcggcg gacaaatcta acccgctgat tgaaaggatg cgtttcctgg
     8341 ggatctattc caataacctt gatgagttct ataaagtccg cttcgctgaa ctgaagcgac
     8401 gcatcattat tagcgaagaa caaggctcca acgctcattc ccgccattta ctgggcaaaa
     8461 ttcagtcccg ggtgctgaaa gccgatcagg aattcgacgg cctctacaac gagctattgc
     8521 tggagatggc gcgcaaccag atcttcctga ttaatgaacg ccagctctcc gtcaatcaac
     8581 aaaactggct gcgtcattat tttaagcagt atctgcgtca gcacattacg ccgattttaa
     8641 tcaatcctga cactgactta gtgcagttcc tgaaagatga ttacacctat ctggcggtgg
     8701 aaattatccg tagcgatagc atccgttacg cgctgctgga gatcccatca gataaagtgc
     8761 cgcgctttgt gaatttaccg ccagaagcgc cgcgtcgacg caagccgatg attcttctgg
     8821 ataacattct gcgttactgc cttgatgata ttttcaaagg cttctttgat tatgacgcgc
     8881 tgaatgccta ttcaatgaag atgacccgcg atgccgaata cgatttagtg catgagatgg
     8941 aagccagcct gatggagttg atgtcttcca gtctcaagca gcgtttaact gctgagccgg
     9001 tgcgttttgt ttatcagcgc gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac
     9061 tgactatttc ccgctacgac tccatcgtcc ccggcggtcg ttatcataat tttaaagact
     9121 ttattaattt ccccaatgtc ggcaaagcca atctggtgaa caaaccactg ccgcgtttac
     9181 gccatatttg gtttgataaa gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg
     9241 atgtgttgct ctattatcct tatcacacct ttgagcatgt gctggaactg ctgcgtcagg
     9301 cttcgttcga cccaagcgta ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt
     9361 cacgcatcat cgactcgatg atccacgccg cacataacgg taagaaagtc actgtggtgg
     9421 ttgagttaca ggcgcgtttc gacgaagaag ccaacattca ctgggcgaag cgcctgaccg
     9481 aagcaggcgt gcacgttatc ttctctgcgc cggggctgaa aattcacgcc aaactgttcc
     9541 tgatttcacg taaagaaaac ggtgaagtgg tccgttacgc acacatcggg accgggaact
     9601 ttaacgaaaa aaccgcgcgt ctttatactg actattcgtt gctgaccgca gatgcgcgta
     9661 tcaccaacga agtacggcgg gtatttaact ttattgaaaa cccataccgc ccggtgacat
     9721 ttgattattt aatggtgtca ccgcaaaact ctcgccgtct gttatatgaa atggtagacc
     9781 gcgaaatcgc caacgcgcag caagggctgc ccagtggtat caccctgaag ctaaataacc
     9841 ttgtcgataa aggcctggtt gatcgtctgt atgcggcctc cagctccggc gtaccggtta
     9901 atctgctggt tcgcggaatg tgttcgctga tccccaatct ggaaggcatt agcgacaaca
     9961 ttcgtgccat cagtattgtt gaccgttacc ttgaacatga ccgggtttat atttttgaaa
    10021 atggcggcga taaaaaggtc tacctttctt ccgccgactg gatgacgcgc aatattgatt
    10081 atcgtattga agtggcgaca ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca
    10141 tcatcgacat attgttcagc gatacggtca aagcacgtta tatcgataaa gaactcagta
    10201 atcgctacgt tccccgcggc aatcgccgca aagtacgggc gcagttggcg atttacgact
    10261 acatcaaatc actcgaacaa cctgaataac cctatgccaa tacacgataa atcccctcgt
    10321 ccgcaggagt ttgctgcggt cgatcttggt tcaaacagtt ttcacatggt catagcccgt
    10381 gtggtagatg gtgccatgca gattattggc cgcctgaaac agcgggtgca tctggcggac
    10441 ggcctggggc cagataatat gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg
    10501 tcgctgtttg ccgaacggct acaagggttt tctcctgcca gcgtctgtat agtcggcacc
    10561 catacgctgc gtcaggcgct gaacgccact gactttctga aacgcgcgga aaaggtcatt
    10621 ccctacccga ttgaaattat ttccggtaat gaagaagccc gtctgatttt tatgggcgtg
    10681 gaacataccc aaccggaaaa aggtcgcaaa ctggttattg atattggcgg cggatctacg
    10741 gaactggtga ttggtgaaaa tttcgaacct attctcgttg aaagccgccg gatgggttgt
    10801 gtcagctttg cccagcttta ttttcctggc ggggtcatca ataaagagaa ttttcagcgc
    10861 gctcgcatgg cggcagcaca aaaactggaa actttaacct ggcaattccg tattcagggc
    10921 tggaacgttg caatgggcgc ttccggtacc ataaaagccg cccatgaagt gttaatggaa
    10981 atgggcgaga aagacgggat aattaccccg gaacgtctgg aaaaactggt aaaagaaatt
    11041 ttacgtcacc gtaatttcgc atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca
    11101 gtcttcgttc ctggactggc gattttatgt ggtgtgtttg atgctttagc catccgtgaa
    11161 ctgcgccttt ctgacggggc gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc
    11221 cgtcatcagg atgtgcgtag tcgcaccgcc agcagcctcg ccaaccagta tcacatagac
    11281 agcgaacagg cccgacgagt gctggatacc actatgcaaa tgtacgaaca gtggcgggaa
    11341 cagcaaccga agctggcgca tccgcaactg gaggcgctac tgcgatgggc cgccatgctg
    11401 catgaggtcg ggttgaatat caaccacagc ggtttgcatc gccactccgc ttatattctg
    11461 caaaacagtg acttgccggg ttttaatcag gaacagcagc tgatgatggc gacactggtg
    11521 cgctatcacc gtaaagcgat taagctcgac gatctgccgc gctttacctt gtttaagaag
    11581 aaacagttcc tgccactgat acagctattg cgccttggcg tattactaaa caatcaacgt
    11641 caggcaacca ccacaccgcc aacattgaca ctgattactg atgacagtca ctggacactg
    11701 cgtttcccgc atgactggtt tagtcagaat gcgctggtac tgcttgatct ggaaaaggag
    11761 caagaatact gggaaggcgt ggctggctgg cggttgaaaa ttgaagaaga aagtacccca
    11821 gaaatcgccg cttaatgctt tgcgggccga cgagaaatgt cggcccgcat tattcaggca
    11881 ctttcgcgaa tgggttcgat ttcattcagc gtatcaatta acggttgcgg cttaccaata
    11941 agataaccct gcatataatc gatccccaaa gagagcaccg cctcgcggat ctcttcgttt
    12001 tcaacgtact ctgccactac ccgcattttc ttcattcgcg ccagatggca aatcgacgcc
    12061 acaatctgat aatccagact atttgacaca atattgcgga taaagctgcc gtcaatttta
    12121 agcagatcgg cattcacatt tttaagccgt gcatagctgg cgtagccggt gccgaaatca
    12181 tcaatcgcaa tctggcagcc taattcctga agatgctgca aggtgatttg cgcctgctta
    12241 acattggtca gagcattact ttcggtgact tcaaaaataa gttgccacgc ttcaatctga
    12301 tatttagcca gcaactgact gacttcaaca ggaaaacgag cctgacatac cgaggttgga
    12361 gacagattaa tagcaaaacg gtgagcgggc atcttcgctc tgttttcagc cataaattgc
    12421 agcgtatgct caatgaccca catgtcgata ctcgacgata aaccaaattc gtgcgcgacc
    12481 ggcaagaagc taccggggcc gatcagttca tcattctcac ctttcatgcg cagaagaatt
    12541 tcatggtaaa catcaccacg cataccggta atcggctggg ccatcaggaa aaaatggttg
    12601 tgttccagcg cctgctgtag tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc
    12661 aaatacattg ccccgcaacg ctgcatattt tccggggcgt tggtcacgat ggaaagttcg
    12721 gcgaccgtat ttagctctcc cagcagcagg tagatatgat tcactggcga gcgcacatag
    12781 cagtaactga cgccaatctg cggttgcatc ggcatcccat cccaaaagaa acgaaattgc
    12841 ttgagatgac tatccagtgc ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc
    12901 gcgagatcgt tacccgaaag ctgataaaca tcttcacccg gttccagcaa gggtgacagc
    12961 cagtgagaaa gtttttgctt
//
LOCUS       DAZSNG010000103        12904 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZSNG010000103 DAZSNG010000000
VERSION     DAZSNG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.104"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHW4Z_003186"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHW4Z_003186"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR8882708.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHW4Z_003187"
     CDS             complement(1806..3224)
                     /locus_tag="ACHW4Z_003187"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8882709.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHW4Z_003188"
     CDS             complement(3378..3722)
                     /locus_tag="ACHW4Z_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR8882710.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHW4Z_003189"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHW4Z_003189"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR8882711.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHW4Z_003190"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHW4Z_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHW4Z_003191"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHW4Z_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR8882712.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHW4Z_003192"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHW4Z_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR8882713.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHW4Z_003193"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHW4Z_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR8882714.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9080..9514
                     /gene="marR"
                     /locus_tag="ACHW4Z_003194"
     CDS             9080..9514
                     /gene="marR"
                     /locus_tag="ACHW4Z_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR8882715.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9534..9917
                     /gene="marA"
                     /locus_tag="ACHW4Z_003195"
     CDS             9534..9917
                     /gene="marA"
                     /locus_tag="ACHW4Z_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR8882716.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9949..10167
                     /gene="marB"
                     /locus_tag="ACHW4Z_003196"
     CDS             9949..10167
                     /gene="marB"
                     /locus_tag="ACHW4Z_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR8882717.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10198..11097)
                     /gene="eamA"
                     /locus_tag="ACHW4Z_003197"
     CDS             complement(10198..11097)
                     /gene="eamA"
                     /locus_tag="ACHW4Z_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR8882718.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11364..12479
                     /gene="ydeE"
                     /locus_tag="ACHW4Z_003198"
                     /pseudo
     CDS             11364..12479
                     /gene="ydeE"
                     /locus_tag="ACHW4Z_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12606..12701
                     /gene="mgtS"
                     /locus_tag="ACHW4Z_003199"
     CDS             12606..12701
                     /gene="mgtS"
                     /locus_tag="ACHW4Z_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR8882719.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12701..12804
                     /gene="mgtT"
                     /locus_tag="ACHW4Z_003200"
                     /pseudo
     CDS             12701..12804
                     /gene="mgtT"
                     /locus_tag="ACHW4Z_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3400 a         3299 c         2950 g         3255 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcacacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcatttga cttatacttg cctgggcaat attaccccct acaactaatt
     9061 acttgccaag gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt
     9121 gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc
     9181 tccgctggat attaccgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg
     9241 tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat
     9301 gctggatcgc ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg
     9361 cggcgtactg gtaaaactta ccaccagcgg cgcggcaata tgcgaacaat gccatcaatt
     9421 agttggccag gacctgcatc aagaattaac aaaaaacctg acggcggacg aagttgcaac
     9481 acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca
     9541 gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg
     9601 aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc
     9661 aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga
     9721 tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac
     9781 gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc
     9841 cgccgcataa ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa
     9901 atcattacaa cagctagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt
     9961 tcatccgcaa tagcagctgc gcttattctc ttttccgcgc agagcgttgc ggaacaaacc
    10021 acgcagccag ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa
    10081 ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg
    10141 ccctattata atcaacacgc tatgtagttt gttctggccc cgacatctcg gggcttatta
    10201 acttcccacc tttaccgctt tacgccaccg caagccaaat acattgatat acagcccggt
    10261 cataatgagc accgcaccta aaaattgcag acccgttaag cgttcatcca acaatagtgc
    10321 cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc gccaggtttc
    10381 atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca caaacgccag
    10441 atacatcagc gacaagatgg tggtcatatc aatagtaacc agactgtgaa tcatggatgc
    10501 ggaaccatcg agaatcagcg agacaacaaa gaagggaatg attgggatta aagcgctcca
    10561 gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt tattgaagat
    10621 gttgccacac gcccaactaa atgctgcagc cagggtcaac ataaagccga gcatcgccac
    10681 atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat
    10741 ccccgccaat tgtttgccat gcagtcgctc cccgaaagta aacgcgccaa gcacgatagt
    10801 aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac caaagttaat
    10861 ggcgcagaac agaaaggcaa actgcgcaaa actgatggtt aatccatacc ccaagagcaa
    10921 attcagtggt actttcggtc gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa
    10981 gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga tgaccacaaa
    11041 atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc gcgacattct
    11101 accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac ttttctttaa
    11161 cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt cgctatactt
    11221 cggatttttg ccatgcgtcc gatggttaat aacaacgacg aggcaagaag ggcgctggta
    11281 gagcgtcgta gggataagtt catttgtctg tccggcagtg cgtttcgtta tgttttattt
    11341 tagagatgta aagaaatagc atggctacgc tgccatttat gaccatttac ttgagtcgcc
    11401 agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc actattggcg
    11461 tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa cgctatatgt
    11521 tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg aataacgtga
    11581 cgctggttgt gctctttttt gccctcatta actgcgccta ttctgttttt gctaccgtgc
    11641 tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca
    11701 actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg ctgttggtga
    11761 tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt cccatgcttt
    11821 tcattcaaat atgggtaaag cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct
    11881 ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc tctggttttc
    11941 tggcttcttt tgtaagcggc gcatttgctt catgcatttc acaatatgtg atggtgattg
    12001 ctgatgggga ttttgccgaa aaggtggtcg cggttgttct tccggtgaat gctgccatgg
    12061 tggttacgtt gcaatattcc gtgggccgcc gacttaaccc agctaacatc cgcgcgctga
    12121 tgacagcagg caccctctgt ttcgtcatcg gactggtcgg ttttattttt tccggcaaca
    12181 acctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc atttatgcgc
    12241 cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc agctattttt
    12301 ccgcccagtc tttaggctgg cttggtgccg cgattaaccc attagtgagt ggcgtagtgc
    12361 taaccagcct gccgccttcc tcgctgtttg tcatcttagc gttggtgatc attgctgcgt
    12421 gggtgctgat gttaaaaggg attcgtgcaa gaccgtgggg gcagcccgcg ctttgttgat
    12481 ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttatctc tgctaatatc
    12541 tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc
    12601 aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt ttattttctg
    12661 gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc
    12721 acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa
    12781 aaaccgccag taaaccggcg gtgaatgctt gcatggatag atttgtgttt tgcttttacg
    12841 ctaacaggca ttttcctgca ctgataacgg atcgttgaca cagtagcatc agttttctca
    12901 atga
//
LOCUS       DAZSNG010000104        12733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZSNG010000104 DAZSNG010000000
VERSION     DAZSNG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.105"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1038
                     /locus_tag="ACHW4Z_003201"
     CDS             <1..1038
                     /locus_tag="ACHW4Z_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR8882720.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACHW4Z_003202"
     CDS             1039..3204
                     /locus_tag="ACHW4Z_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR8882721.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACHW4Z_003203"
     CDS             3218..4129
                     /locus_tag="ACHW4Z_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR8882722.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACHW4Z_003204"
     CDS             4129..5424
                     /locus_tag="ACHW4Z_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR8882723.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACHW4Z_003205"
     CDS             5469..5699
                     /locus_tag="ACHW4Z_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882724.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACHW4Z_003206"
     CDS             5677..6177
                     /locus_tag="ACHW4Z_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR8882725.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACHW4Z_003207"
     CDS             6177..7595
                     /locus_tag="ACHW4Z_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR8882726.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACHW4Z_003208"
     CDS             7595..8443
                     /locus_tag="ACHW4Z_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR8882727.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACHW4Z_003209"
     CDS             8443..8898
                     /locus_tag="ACHW4Z_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR8882728.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACHW4Z_003210"
     CDS             8901..9593
                     /locus_tag="ACHW4Z_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR8882729.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACHW4Z_003211"
     CDS             9603..10934
                     /locus_tag="ACHW4Z_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR8882730.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACHW4Z_003212"
     CDS             10935..12665
                     /locus_tag="ACHW4Z_003212"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR8882731.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3435 a         3144 c         3355 g         2799 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAZSNG010000105        12695 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZSNG010000105 DAZSNG010000000
VERSION     DAZSNG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.106"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHW4Z_003213"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHW4Z_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR8882732.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHW4Z_003214"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHW4Z_003214"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR8882733.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHW4Z_003215"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHW4Z_003215"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR8882734.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHW4Z_003216"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHW4Z_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR8882735.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHW4Z_003217"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHW4Z_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR8882736.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHW4Z_003218"
     CDS             4025..4615
                     /locus_tag="ACHW4Z_003218"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR8882737.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHW4Z_003219"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHW4Z_003219"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR8882738.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHW4Z_003220"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHW4Z_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR8882739.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHW4Z_003221"
     CDS             complement(6551..6658)
                     /locus_tag="ACHW4Z_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR8882740.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHW4Z_003222"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHW4Z_003222"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8882741.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHW4Z_003223"
     CDS             7648..7875
                     /locus_tag="ACHW4Z_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR8882742.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHW4Z_003224"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHW4Z_003224"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR8882743.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="ACHW4Z_003225"
     CDS             complement(9447..10703)
                     /locus_tag="ACHW4Z_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFR8882744.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="ACHW4Z_003226"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="ACHW4Z_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR8882745.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="ACHW4Z_003227"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="ACHW4Z_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR8882746.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="ACHW4Z_003228"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="ACHW4Z_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8882747.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3241 c         3199 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAZSNG010000106        12648 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZSNG010000106 DAZSNG010000000
VERSION     DAZSNG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.107"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHW4Z_003229"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHW4Z_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8882748.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHW4Z_003230"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHW4Z_003230"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR8882749.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHW4Z_003231"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHW4Z_003231"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR8882750.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHW4Z_003232"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHW4Z_003232"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR8882751.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHW4Z_003233"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHW4Z_003233"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR8882752.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACHW4Z_003234"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACHW4Z_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACHW4Z_003235"
     CDS             7850..7903
                     /locus_tag="ACHW4Z_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882753.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHW4Z_003236"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHW4Z_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR8882754.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACHW4Z_003237"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACHW4Z_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR8882755.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHW4Z_003238"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHW4Z_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR8882756.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="ACHW4Z_003239"
     CDS             10967..12166
                     /locus_tag="ACHW4Z_003239"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR8882757.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3112 a         3170 c         3359 g         3007 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DAZSNG010000107        12389 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZSNG010000107 DAZSNG010000000
VERSION     DAZSNG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.108"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHW4Z_003240"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHW4Z_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR8882758.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHW4Z_003241"
     CDS             complement(2005..2928)
                     /locus_tag="ACHW4Z_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8882759.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHW4Z_003242"
     CDS             3145..4488
                     /locus_tag="ACHW4Z_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8882760.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHW4Z_003243"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHW4Z_003243"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR8882761.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHW4Z_003244"
     CDS             6508..6732
                     /locus_tag="ACHW4Z_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR8882762.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHW4Z_003245"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHW4Z_003245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR8882763.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHW4Z_003246"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHW4Z_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR8882764.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHW4Z_003247"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHW4Z_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR8882765.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHW4Z_003248"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHW4Z_003248"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR8882766.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="ACHW4Z_003249"
     CDS             10315..10971
                     /locus_tag="ACHW4Z_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR8882767.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_003250"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12389
                     /locus_tag="ACHW4Z_003251"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="ACHW4Z_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3230 a         2903 c         3110 g         3146 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DAZSNG010000108        12220 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZSNG010000108 DAZSNG010000000
VERSION     DAZSNG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.109"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..492)
                     /locus_tag="ACHW4Z_003252"
     CDS             complement(<1..492)
                     /locus_tag="ACHW4Z_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8882768.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACHW4Z_003253"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACHW4Z_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8882769.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACHW4Z_003254"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACHW4Z_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8882770.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="ACHW4Z_003255"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="ACHW4Z_003255"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR8882771.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACHW4Z_003256"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACHW4Z_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR8882772.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="ACHW4Z_003257"
     CDS             3646..4155
                     /locus_tag="ACHW4Z_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8882773.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACHW4Z_003258"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACHW4Z_003258"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR8882774.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="ACHW4Z_003259"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="ACHW4Z_003259"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR8882775.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="ACHW4Z_003260"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="ACHW4Z_003260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR8882776.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="ACHW4Z_003261"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="ACHW4Z_003261"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR8882777.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="ACHW4Z_003262"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="ACHW4Z_003262"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR8882778.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="ACHW4Z_003263"
     CDS             complement(8797..9690)
                     /locus_tag="ACHW4Z_003263"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR8882779.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="ACHW4Z_003264"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="ACHW4Z_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="ACHW4Z_003265"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="ACHW4Z_003265"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR8882780.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12220
                     /gene="allE"
                     /locus_tag="ACHW4Z_003266"
     CDS             11580..>12220
                     /gene="allE"
                     /locus_tag="ACHW4Z_003266"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR8882781.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2967 a         3158 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttataa
//
LOCUS       DAZSNG010000109        12152 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZSNG010000109 DAZSNG010000000
VERSION     DAZSNG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.110"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..995
                     /locus_tag="ACHW4Z_003267"
     CDS             126..995
                     /locus_tag="ACHW4Z_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR8882782.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHW4Z_003268"
     CDS             complement(1198..1551)
                     /locus_tag="ACHW4Z_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR8882783.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHW4Z_003269"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHW4Z_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR8882784.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHW4Z_003270"
     CDS             complement(3379..3672)
                     /locus_tag="ACHW4Z_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR8882785.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHW4Z_003271"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHW4Z_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR8882786.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHW4Z_003272"
     CDS             complement(5220..5696)
                     /locus_tag="ACHW4Z_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR8882787.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHW4Z_003273"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHW4Z_003273"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR8882788.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHW4Z_003274"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHW4Z_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR8882789.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHW4Z_003275"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHW4Z_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR8882790.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHW4Z_003276"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHW4Z_003276"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR8882791.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWLIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHW4Z_003277"
     CDS             11052..11627
                     /locus_tag="ACHW4Z_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8882792.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHW4Z_003278"
     tRNA            11734..11809
                     /locus_tag="ACHW4Z_003278"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2980 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggctgatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa ca
//
LOCUS       DAZSNG010000110        12114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZSNG010000110 DAZSNG010000000
VERSION     DAZSNG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.111"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..371
                     /locus_tag="ACHW4Z_003279"
     CDS             <1..371
                     /locus_tag="ACHW4Z_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882793.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHW4Z_003280"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHW4Z_003280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR8882794.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHW4Z_003281"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHW4Z_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR8882795.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHW4Z_003282"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHW4Z_003282"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR8882796.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHW4Z_003283"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHW4Z_003283"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR8882797.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHW4Z_003284"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHW4Z_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR8882798.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHW4Z_003285"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHW4Z_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR8882799.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHW4Z_003286"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHW4Z_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR8882800.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHW4Z_003287"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHW4Z_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR8882801.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHW4Z_003288"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHW4Z_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR8882802.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHW4Z_003289"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHW4Z_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR8882803.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHW4Z_003290"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHW4Z_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR8882804.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="ACHW4Z_003291"
     CDS             complement(11913..>12114)
                     /locus_tag="ACHW4Z_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR8882805.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2934 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DAZSNG010000111        12075 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZSNG010000111 DAZSNG010000000
VERSION     DAZSNG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.112"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..346
                     /locus_tag="ACHW4Z_003292"
                     /pseudo
     CDS             1..346
                     /locus_tag="ACHW4Z_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(432..995)
                     /locus_tag="ACHW4Z_003293"
     CDS             complement(432..995)
                     /locus_tag="ACHW4Z_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR8882806.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(1042..2403)
                     /locus_tag="ACHW4Z_003294"
     CDS             complement(1042..2403)
                     /locus_tag="ACHW4Z_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR8882807.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2455..2685)
                     /locus_tag="ACHW4Z_003295"
     CDS             complement(2455..2685)
                     /locus_tag="ACHW4Z_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882808.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(2909..3028)
                     /locus_tag="ACHW4Z_003296"
                     /pseudo
     CDS             complement(2909..3028)
                     /locus_tag="ACHW4Z_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3723..3914)
                     /locus_tag="ACHW4Z_003297"
     CDS             complement(3723..3914)
                     /locus_tag="ACHW4Z_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882809.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3911..4333)
                     /locus_tag="ACHW4Z_003298"
     CDS             complement(3911..4333)
                     /locus_tag="ACHW4Z_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR8882810.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4380..4805)
                     /locus_tag="ACHW4Z_003299"
     CDS             complement(4380..4805)
                     /locus_tag="ACHW4Z_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8882811.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5223..5999)
                     /locus_tag="ACHW4Z_003300"
     CDS             complement(5223..5999)
                     /locus_tag="ACHW4Z_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882812.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(6050..6484)
                     /locus_tag="ACHW4Z_003301"
     CDS             complement(6050..6484)
                     /locus_tag="ACHW4Z_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR8882813.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6498..6719)
                     /locus_tag="ACHW4Z_003302"
     CDS             complement(6498..6719)
                     /locus_tag="ACHW4Z_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882814.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6720..7403)
                     /locus_tag="ACHW4Z_003303"
     CDS             complement(6720..7403)
                     /locus_tag="ACHW4Z_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8882815.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7480..7773)
                     /locus_tag="ACHW4Z_003304"
     CDS             complement(7480..7773)
                     /locus_tag="ACHW4Z_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882816.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7920..8882
                     /gene="parM"
                     /locus_tag="ACHW4Z_003305"
                     /gene_synonym="stbA"
     CDS             7920..8882
                     /gene="parM"
                     /locus_tag="ACHW4Z_003305"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR8882817.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8885..9235
                     /locus_tag="ACHW4Z_003306"
     CDS             8885..9235
                     /locus_tag="ACHW4Z_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8882818.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9358..9639)
                     /locus_tag="ACHW4Z_003307"
     CDS             complement(9358..9639)
                     /locus_tag="ACHW4Z_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882819.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9767..10001)
                     /locus_tag="ACHW4Z_003308"
                     /pseudo
     CDS             complement(9767..10001)
                     /locus_tag="ACHW4Z_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11450..12075)
                     /locus_tag="ACHW4Z_003309"
                     /pseudo
     CDS             complement(11450..12075)
                     /locus_tag="ACHW4Z_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT         2920 a         3277 c         3029 g         2849 t
ORIGIN      
        1 ggatgccggg agcgcaggcc acggcgcgac aagggtggcg cgaccgttcc ggtcgctgca
       61 aacccgggtt tttgcgtcag ggatggaagc ccgccagggc ggagacagct ccgctggctc
      121 cgttcacgac agcccgcccc gccaggggga cgcccggaaa aggcataagc aggagaacaa
      181 caaaacagac agtaatcaga gcagcagaaa aaagaagaac agtagcagta gcagtagcag
      241 tagcagtagc aggaacaaca acatcatcag taacaggcaa atgacaggtc tgaaaagaca
      301 gaagcccaca gcagaacgtc cgcgtaatga aaggggcggc gcgtaagcgc ggcccctttc
      361 agcgccaccg gcgcgcggtc ttttcccggc gcgggcagac gggcatttat cctcccgcct
      421 gcccggattc atcaggcacg cagacgaata atcatcgtcg gaacacgggt ataagcaaat
      481 gtgccgcacg gcagcacctc gcggacatca gaaaacggta acagcttctc ctgctggcgc
      541 ggcccgttca gacagacggc aaccagaacg ccccccggac gcaacaggga aaaagcccgc
      601 aggatatgcc ggatatcctg cccgtgactg aacggcggat tcataatgat ccggctgtaa
      661 tactgcaccg gctgccattc catgaagtcg ccacactgta ccctgacacc gttaaagttt
      721 tcccgcagat accggaccag cccgctgtta atctccaccg catcacacat ggcctccggc
      781 gcggtatccc gaatcgcccg cagtatggcc ccggtgccgg cagagggttc cagaatgtga
      841 tcacggttgc tgatttccgc cagcgtcacc aggcgatcac agaccggggc aggagtcaca
      901 aaaagctgag attcagcccg gatttcagca accggcgcgg cgttatccag ccttgcgcgc
      961 atcagccgga tacgttctct taactgactg tgcatgtcat tatctccgtc atcccccggg
     1021 gatttcccgg ggggatatac cttatttctg cgggatttcc gtgattttca tgtcggtgat
     1081 atacacattc accagacgga aattattgtc cattgtgcgg cggtagcggt atgccccgtg
     1141 gtcttctgct tcttccacac tgcgcacggc cttacagtcc cggggcaggg cggcccactg
     1201 cgcctttgtc atttcccgga aaccttcccc cggatagttg actaccggcg gacgcttcgc
     1261 ggcctgactg gcgacggcag cctcttctgc cgatggtgct ttgtagtccg tgatgcgatc
     1321 gggcgtcact ttcatcgtgc cgctgtaccc gagaaaactg taatttggcg ttgtgactga
     1381 actcaccgcc ccgttgcttt tgttcacgcg gatgatagtc agccactcgc cccggctgaa
     1441 aacctgtccg cccggctcaa aatcctgtgt ccgggtaacc acgccgccgc tttcgtccag
     1501 catggcacgc tcatagatca ggcggttctg atagtggtta acccagcgtt gctgatgctg
     1561 aatctgccgt tcatgacagc ggatcgcaat ttcgcgggcc tgctccgtgg tgatgatgtc
     1621 atcatccagg gccgaccata acgaccggct cccttcatac tggctttttt ctgccgggcg
     1681 cggatacgta tccagcggga aacaggcgct gatatggtca tgactgctga tgagttttgc
     1741 catattcaga tccagcgatt cagcccgcca catcgtcaga tatttctgcg actgtgcgat
     1801 ggtcttttca gccttgcgca aatccgcttc gatttttttg atacgacgcc agcgaacgtc
     1861 cgggcgttct ttatacttcg cgtgaagcag tgccgaccgc gcccgctcct cccagtattc
     1921 cgcacgttca aagagcatca cggcacgttt catgccgttt tcaatacgct gcgcatcacg
     1981 acgggcgcgg cgttcgctgt gatgccccac aagaatgggc tgaccgggcg ggatcatcgc
     2041 ggccagtctt tccacttcat caagtgcctg tgcggattca ctggcccgct ttccgctgta
     2101 tccggtaaac cgctccgccc gtgcttcctg acgttcagcg agcgtgctgt cttcatcctc
     2161 aatctctccg gcaagtgaga ggagcacgtc ttcccggccc ggtgtccagg ccggcgcgac
     2221 aaacagcgcc tgtttgggtg cccagcgaaa accggcatca tgcacttttt tatacgtctc
     2281 cgggtcaaga cgtgacgcgg catacaggcg cagtttgttg tcatccggtg agtaggtcgc
     2341 gcggtacatc tgcacagggc tggcggcttc cggggcggtc atggtgattg ctgctaatgt
     2401 cataaaatgt tctccttccg tctgttgccg cccctcgcgg ggcggctcac ctcatcagtt
     2461 aaaagcgcgt ttcagttcga gtgtgcggag cacctgcccg aaagccgtgt ttttcatggc
     2521 ggggatgtgt acaccacgcc cccggtgaaa actccgggct atgtgtcgcc cgaaggcttt
     2581 caacacgggg tcaatttccg gcgtggggat gtcttcgcgt ctgaaacggg gatgacggcg
     2641 gggctttaca gccgctgcgg aaggtgaaac aatgcttaca gtcataaatg gattccttat
     2701 gacaaattgg ttaacaatgc cgtcaaaatt ggtcgcacaa ttcttacggc gtggattaag
     2761 caggtgcctg acctgctttt ttcatatctg catctgacgc tttccggtgt tttcctcctt
     2821 ctgttcctgc gttctcatta cctgtcagcc ggatgactga cagaacaaat aaatgcgtat
     2881 tgccggagcc tgaaataaat cctgattttt aaagaacatc acccgttgag tttcgggagc
     2941 cgcggttttg acgtttcgcg ggaacctgct gctgtattca ctgtggtatg aacttcaccc
     3001 gtttgagaac cttcgccgca aggggcatca ctgccacggc ctgacgcacc tgtcctgggc
     3061 agaacgcaaa attttttacc gggttttggg taaaaaattt tgtgggctgt tcatttaccc
     3121 tggacagtca gtgcggcagg ttgtggctgt atcagccccc ggcgacaggg actcatacgg
     3181 gtgtgtggca cccggcaggc aatgagcagg ggcccgccgc aacgacggag agcgcgtgcg
     3241 cgaaacggcg ttgcccctta catcccggcg gccttttgcc gggatgccgg gagcgcaggc
     3301 cacggcgcga cagaggcggc gcgaccggaa cggtcgctgc aagccagggg ttttcacgca
     3361 tcggaccgta gcggcagccc gaaaccgcgc cagcgggttc ggcggagact ggcaggtgcg
     3421 ccagcagctg cctgtcggag tcgggcctga cgaccggatg ccgccaggca gccgggagcc
     3481 gaaggtgcgc ccggcagaca gaacaacact gcagtagtca gaatcagagc agtagataaa
     3541 ggaacaacaa catcaggaac agttccataa cataagaaag aaacactcct gagtcagaca
     3601 catcacagac gggaagggta acgggcgacg cgccagcgtg gcccgcttcc cttccggcag
     3661 gcgtcagcct gccaggggtt ttcgcgcggg ctgacaggcg gcctccaccg cctgcccctg
     3721 tatcacctgt ttatgctgcc ctgaaggatg tagtgaccgc cgaggaatac agcctgctga
     3781 ttcgccacaa aatcgcagat ttcatcaaca atctgcgcag tggtgagcgg tttctgcccc
     3841 atttcacgac gccgcgcatt aatcacggct ttgtaattgc gcagaatttc gcgggtttct
     3901 gtgctgatat tcatgacatg tgctccccgt ccagcgcctg taacacaacg tccattgctt
     3961 cgcgttcttc cctgacgaat gcgtcaccgt cgcccccgtt ctcgctcccg acggcataca
     4021 ggcgcttcat ttcactgcct gccagcgcca tcactttttc cagcatcacc gccggaactg
     4081 tcacctgacg gctggcgcgc ttttcttcca tcagctcacg aacaacgtcg tgaacaatgc
     4141 tgacatccgc accatgcgca aaggcatcgt ccagtcgctg catgacggtt tccagctcat
     4201 cgtctgtcag actccagtcc tgggctatgt gacgcacatc cccacgggtc agcaccacgg
     4261 caaaaacgca ttcactgtcc ggcacgtctg tgttcagcac ttcgcggata atttctttaa
     4321 cagtacagta catgttctcg tctcctttac aggggcggca ttgccgcccc gtccgttaat
     4381 cagtccagtg ccgcgtaaat cgcgttgcat tcggggtgaa attcgatgtg acgccacaac
     4441 tgcgcatcgc gcatccggta aagctgggtc agtcctgcat ccccgctgtc gtggtacagc
     4501 cacaactggc ggttaatcac aagggaagtc agaatgatgc ctgcggcatc agcactcacg
     4561 gtacggtcaa accagtttgc accgttcacc atgtggaaac ggtcgccatc cggcatcatg
     4621 taaccgccac cgtccggcag gcggatgaag tgccagaagc ctccctgata ttccggcatc
     4681 attttttgcg ccagcgcgta cacatacgcc tcggcataca gaaaatcgtt gccaaacaga
     4741 tacggtagaa aggagagacg gtcgcactct tcaacgttca gagtgacagg tttcgcatat
     4801 tgcatgatgt tttcctccgt tgttcacata ttccgcgaca ccgggcggtg ccgtgcctcc
     4861 ataaaagccg ggcgataagg cggggatgca cagggcgcaa gggaagccgc agcctgacgc
     4921 acacggctgg cgggagttgg agggtgagac cgcgaaatct gagcgggtga acgaccggaa
     4981 aatcacggca gacgggaagg agggaccggg tgaccgcagc ggcgaagcgt acccttgcat
     5041 ccctgccagc ccggctaaga tggaggcacg gcacagcccg cacacgacgc tgaggccaca
     5101 taactgaaaa accacaaggc catgccctgc atggccgggt aaagcggagc cggcggcgac
     5161 ttcctttgtg ctctgtccgg ggaccggcag gaaaaaggcg gggatttacc ccgccaacgt
     5221 catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc ccgggcttcc
     5281 cggaccacct cccgcagaac accacggtca aaccagcggc ggggcggttc atccggtata
     5341 cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc gctgaccagg
     5401 tacatttcca ccgacgaaaa acgtgctccc cggttcacga tggcatcgag caccacgggc
     5461 aggttttcgt catcttcatc gagccagcag gggtgatcgc tttccttcac aaccagacga
     5521 aagcggcgcg tctggcgagt cagcaacgtc tgaccggtgg tggtcagcgc cgcccggata
     5581 cgccgcgcat cttctgccac ccggcaactg acactgcgta ccgcatcgcg caccgccgca
     5641 agtacagccc tgatttcgtc ttcggtcagc cattctccgg catacgggaa agacacctgc
     5701 gttccgtctg ccggaagtac cggcagcgac tgccactgac tgccggacag taccagttcc
     5761 gccccggttc cggtcagttt ctgaatggcg gaggccagga gcggcggcag tacgccgccg
     5821 tgacggttga taagtaacgt cgccagccgg ggcgcatcca gcagaacata aatgctgatc
     5881 cagcccggat acttcgggtt acgctggtca acatccgtct cttccggcca gccctgcgcc
     5941 agcagcaggt tgcgggcgtg acgacaaatc agtgcgttca gcgtgctgtt ttcgttcatg
     6001 attaagcgtt ctccctgtat cgccgcccgc agggcaggcg gcgataacat cagtcgtttt
     6061 cgtgtgtgtt gtttttcagc agggtgcggg cctgattaac cacctcctga cggcgggtga
     6121 cgcattccgg gacggcgagg ccatgatccc gggcggccca ttcacgtttc cacaatgcct
     6181 gttcagcggt ctgaatcagg gacgccagca gttcagcagg gacggtcacc aggcggtttt
     6241 cgctctcgtt gcggataatc tccatcaccg cagcggaaga aataccggac tcaatccgct
     6301 ggtcttccgg gatgtcctcc aggcgtgcca ggacggtcct gatttcatga ccggtcagag
     6361 aaatttccat accgtcagcc agtgcctgaa tctcttccgg ggaccagata accagcatca
     6421 gcggcatgtc tcccggatac tttactgcca gctcccggca tagcgtttcg catgttccgt
     6481 acattctcgt ctccttctca atctttaaag cacgcttcca gttccagaat ttcggtatat
     6541 gcctccatca ggaggcggtt tgcgcgaaac agcgtctgac gcaccagacg ttccggcagg
     6601 gtgtcggcat taaagctgta ccgctgctcc atctcattca tggttgtacg caggtcgcac
     6661 atggcattgg ccacctccgc cacttcggcg cgcagttttt cgtgtcctgc atagttcatt
     6721 tacgcagcct ccggcataaa ccagtcatca ttcgcggccc cctgctgcat ggcccgttgc
     6781 acggcggcaa ggcgttgctg cccggcacgg tgatactgct caagcagctc gataccgata
     6841 taccggcgtc cggactggag ggcggcgacg caggttgagc cgctgcctgc aaacgggtcc
     6901 agcacaattg cgttcgggtg tgtgaagctc tcaatcagcg gttgcaggct ggtaacgggc
     6961 ttttccgtcg ggtgatggcg attgcccgaa tatttccagc ccagcacgtc cggcagcggg
     7021 ttttgtggca gacgtggacg gccttttgcc aggatgtagg cacattcgtg gcgatagccc
     7081 acatatgcgg ccttcgatgt gtagtttttg gtgaagacca ggtgaccaac aacgctgaat
     7141 cccgcatttt tccaggcggc cataaagcga tcgacgcggt tccagccata gaagctcacc
     7201 attaacgcgt cttttttgag tacgcggtac atttcattac aggctggttg cagccattca
     7261 tcggttttat cgccggcgat ggtgcgcccg gaacggtcac ggaaaccgac gagatacggc
     7321 gggtcggtga ggatgaaatc gacagcattg cccggaaacg tggccataac acgcacgcag
     7381 tcacccagga taaaacggga cataacagca ctccttctct tacagagggc accgggaggg
     7441 cacccctgtg gtgcccgttc cccgctgcct gtgggctgtt caccgcgtcc ggtaacgggt
     7501 caggccgacc gtggaatacc gcagccgtcg tctttttgac ggcgaggata tgccgcgaaa
     7561 gcggcttgaa gggcgttcat cagaaaaggg cgacgggctg gcgtacactg tcaccaacgc
     7621 cagccgggag cggtaaaagc gcccgtcccg tgcccggccg gacgcgggac actgaccaca
     7681 ggcagcgttc ctgccgcgcc gcgtcagccg gcgcagtaaa aagaggagcc cggcgacatc
     7741 ttttgttacc cgccaaacaa aacccaaaaa caacccatac ccaacccaat aaaacaccaa
     7801 aacaagacaa ataatcattg attgatggtt gaaatggggt aaacttgaca aacaaaccca
     7861 cttaaaaccc aaaacatacc caaacacaca ccaaaaaaac accataagga gttttataaa
     7921 tgttggtatt cattgatgac ggttcaacaa acatcaaact acagtggcag gaaagcgacg
     7981 gaacaattaa acagcacatt agcccgaaca gcttcaaacg cgagtgggca gtctcttttg
     8041 gtgataaaaa ggtctttaac tacacactga acggcgaaca gtattcattt gatccaatca
     8101 gcccggatgc tgtagtcaca accaatatcg catggcaata cagcgacgtt aatgtcgttg
     8161 cagtgcatca cgccttactg accagtggtc tgccggtaag cgaagtggat attgtttgca
     8221 cacttcctct gacagagtat tacgacagaa ataaccaacc caatacggaa aatattgagc
     8281 gtaagaaagc aaacttccgg aaaaaaatta cattaaatgg cggggataca ttcacaataa
     8341 aagatgtaaa agtcatgcct gaatctatac cggcaggtta tgaagttcta caagaactgg
     8401 atgagttaga ttctttatta attatagatc tcgggggcac cacattagat atttctcagg
     8461 taatggggaa attatcgggg atcagtaaaa tatacggaga ctcatctctt ggtgtctctc
     8521 tggttacatc tgcagtaaaa gatgcccttt ctcttgcgag aacaaaagga agtagctatc
     8581 ttgctgacga tataatcatt cacagaaaag ataataacta tctgaagcaa cgaattaatg
     8641 atgagaacaa aatatcaata gtcaccgaag caatgaatga agcacttcgt aaacttgagc
     8701 aacgtgtatt aaatacgctc aatgaatttt ctggttatac tcatgttatg gttataggcg
     8761 gtggcgcaga attaatatgc gatgcagtaa aaaaacacac acagattcgt gatgaacgtt
     8821 ttttcaaaac caataactct caatatgatt tagttaacgg tatgtatctc ataggtaatt
     8881 aatgatggac aagcgcagaa ccattgcctt caaactaaat ccagatgtaa atcaaacaga
     8941 taaaattgtt tgtgatacac tggacagtat cccgcaaggg gaacgaagcc gccttaaccg
     9001 ggccgcactg acggcaggtc tggccttata cagacaagat ccccggaccc ctttcctttt
     9061 atgtgagctg ctgacgaaag aaaccacatt ttcagatatc gtgaatatat tgagatcgct
     9121 atttccaaaa gagatggccg attttaattc ttcaatagtc actcaatcct cttcacaaca
     9181 agagcaaaaa agtgatgaag agaccaaaaa aaatgcgatg aagctaataa attaattcaa
     9241 ttattattga gttcccttta tccactatca ggctggataa agggaactca atcaagttat
     9301 tttcttacca gtcattacat aatcgttatt atgaaataat cgtttgcact gtctctgtta
     9361 ttcaggcaat ttcaataaag gcacttgctc acgctctgtc attttctgaa actcttcatg
     9421 ctgcatttcg atttttcttc taaaatcata acttgcatac ctgttctttt tatgtatatc
     9481 cagatctatt tttgtgacga gttcaaacca caatgatagc actccccatg cagcgttatg
     9541 aaacatttcc gcaacagatt tagagtctgt attaaccgct tcagtcatgt agtcgctaat
     9601 acgttctgca gtgattttcg ttatttcatc gtaattcatt ttatccccta caagactatt
     9661 taatggaatg aaaaatattt ggtctgataa caatgctcct gaattaacac ctgcaggcta
     9721 tgttaaagtt gccctcacac acaatctacc tgttttctcg tgtgtgtcac aggcgcaata
     9781 cataaatagt ctggccatta agttcgtaac ggatttcgta ctcgccagca aaaatccgtc
     9841 tgacatccct ggtttcaaac tgaaacaact gttcatccca tacgtggatg agttaacaga
     9901 atgaccggcg cctgtgtcag ggactacacc gttttggctg cagctggttt actgaccaac
     9961 gccagaaaat catataactg cgtcaatgtc agcgtatcca gcgcgactgt attatttttc
    10021 tctgcaacca gaatattgca ttaggttatt accaggtgtt ctaatgtaca ggtaaagacg
    10081 ttctttataa tctgaaatct ctacttttaa tccctcagac tttgcctgct gaaagccctc
    10141 gtcaaggtgc gcttttatta ggttaatgtc tggatgtctt gtatcggggg caattctgta
    10201 ttggtgtaac gatggggtgg atgttccctg caataagacc tccagataga gattggcaca
    10261 accattccca ttatttctaa tagtttattg gtaatcacaa actgtatatt tagccattat
    10321 attgaatcct gtgaagtaaa cggcgacggc tcaaaatttt atcaaaccta agataattct
    10381 accatcaaag ttaataattt gaatttatat gacggctcta tgggggggta aaaatccccg
    10441 tgcagagagg cgattttcag agttgaaaac gtcgtcgtta agaacaccaa agtcagtacc
    10501 accaacccgg aaagtagctt catgcaccgg gacaacagca gttttggtgt ccgaaatcgt
    10561 acaattctgt acatctacgc aaggaaatgt acggaagtaa gccaaatggt tatcggacgg
    10621 tcggaacatt tcgtacagcc tttacgttca gtgagttagc tggcctagat gtaaccactc
    10681 gttcaactca attctcttag cgtgggaaaa tttccgtttt ccaagcggcc ccctcaagcg
    10741 gaccccttat agtacaataa ttttcgatat ccaaactgcc cccaattaca tctccagtct
    10801 ctggcagcag agatcatata tttccattgc cgccgtagcc acgatattct ggctattcac
    10861 aaaaacaatt tagccccaaa gcgcagtaaa aaagtacact atgaaggtgt atgatacctt
    10921 catagtgctt aagtaattat gagtaatata atctgtgtaa actactaatt gcttcaccgt
    10981 cagtgattaa atgcgtagct gttccttgtc ttatataata cctattttca aaatgacaga
    11041 cttctccgac actttttatt tcgattttta aaacatgcag tccttcatat gaacataatg
    11101 aaatatcttt tagtatttgt tgtttcaaag cctcattaaa attaaatgat tctatttttt
    11161 gttttatgaa cagaaagtaa ttatctatgt tttttgattg aattacagct tcatgatcaa
    11221 ttccattgat ataatagcca ttgcactcaa taggggtaac accatataaa gtcttaactc
    11281 tgtctgccgt agctttggta tcggaaacac caactaaaat ataaccatta gattctctac
    11341 cgatgttatt tatggcgaca gccgtttgga ctatattaat gaaacattct tcgtctattt
    11401 tattctcgcc tgagagaata aataatccct gtttggtaat gactccaact tattgatagt
    11461 gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg
    11521 acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa
    11581 ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca
    11641 gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac
    11701 gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagcc
    11761 tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc cccgacgtat ccccactgtt
    11821 cgtccatttc cgcgcagacg atgacgtcac tgcccggctg tatgcgcgag gttaccgact
    11881 gcggcctgag ttttttaaat ggcggaaaat cgtgttgagg ccaacgccca taatgcgggc
    11941 ggttgcccgg catccaacgc cattcatggc catatcaatg attttctggt gcgtaccggg
    12001 ttgagaagcg gtgtaagtga actgcagttg ccatgtttta cggcagtgag agcagagata
    12061 gcgctgatgt ccggc
//
LOCUS       DAZSNG010000112        12062 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZSNG010000112 DAZSNG010000000
VERSION     DAZSNG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.113"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="ACHW4Z_003310"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHW4Z_003310"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="ACHW4Z_003311"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="ACHW4Z_003311"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR8882820.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="ACHW4Z_003312"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="ACHW4Z_003312"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR8882821.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="ACHW4Z_003313"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="ACHW4Z_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="ACHW4Z_003314"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="ACHW4Z_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR8882822.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="ACHW4Z_003315"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="ACHW4Z_003315"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR8882823.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="ACHW4Z_003316"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="ACHW4Z_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR8882824.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="ACHW4Z_003317"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="ACHW4Z_003317"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR8882825.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="ACHW4Z_003318"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="ACHW4Z_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR8882826.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /gene="agaW"
                     /locus_tag="ACHW4Z_003319"
     CDS             9553..10332
                     /gene="agaW"
                     /locus_tag="ACHW4Z_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR8882827.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /gene="agaE"
                     /locus_tag="ACHW4Z_003320"
     CDS             10322..11131
                     /gene="agaE"
                     /locus_tag="ACHW4Z_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR8882828.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /gene="agaF"
                     /locus_tag="ACHW4Z_003321"
     CDS             11218..11652
                     /gene="agaF"
                     /locus_tag="ACHW4Z_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR8882829.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="ACHW4Z_003322"
     CDS             11649..>12062
                     /locus_tag="ACHW4Z_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8882830.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3252 c         3077 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAZSNG010000113        12003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZSNG010000113 DAZSNG010000000
VERSION     DAZSNG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.114"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2252)
                     /locus_tag="ACHW4Z_003323"
     CDS             complement(<1..2252)
                     /locus_tag="ACHW4Z_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR8882831.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="ACHW4Z_003324"
     CDS             complement(2276..2899)
                     /locus_tag="ACHW4Z_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR8882832.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="ACHW4Z_003325"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="ACHW4Z_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="ACHW4Z_003326"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="ACHW4Z_003326"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR8882833.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /gene="ubiG"
                     /locus_tag="ACHW4Z_003327"
     CDS             7507..8229
                     /gene="ubiG"
                     /locus_tag="ACHW4Z_003327"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR8882834.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11932)
                     /gene="yfaL"
                     /locus_tag="ACHW4Z_003328"
                     /pseudo
     CDS             complement(8357..11932)
                     /gene="yfaL"
                     /locus_tag="ACHW4Z_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2783 a         3325 c         2954 g         2941 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgta ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg ttataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tgacgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcggcgata gactggcccc
    11941 ctgaatctcc agacaaccag tatcacttat ttaagtgata gtcttaatac tagtttttag
    12001 act
//
LOCUS       DAZSNG010000114        11776 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZSNG010000114 DAZSNG010000000
VERSION     DAZSNG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.115"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..852
                     /locus_tag="ACHW4Z_003329"
     CDS             <1..852
                     /locus_tag="ACHW4Z_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR8882835.1"
                     /translation="IRHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGF
                     NILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAE
                     IVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHE
                     IYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHN
                     PLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTG
                     TIYPIEK"
     gene            874..2283
                     /locus_tag="ACHW4Z_003330"
     CDS             874..2283
                     /locus_tag="ACHW4Z_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882836.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="ACHW4Z_003331"
     CDS             2261..3040
                     /locus_tag="ACHW4Z_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR8882837.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="ACHW4Z_003332"
     CDS             3037..3897
                     /locus_tag="ACHW4Z_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR8882838.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="ACHW4Z_003333"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="ACHW4Z_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="ACHW4Z_003334"
     CDS             complement(4637..4897)
                     /locus_tag="ACHW4Z_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8882839.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="ACHW4Z_003335"
     CDS             complement(4888..6159)
                     /locus_tag="ACHW4Z_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8882840.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGV
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="ACHW4Z_003336"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="ACHW4Z_003336"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR8882841.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="ACHW4Z_003337"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="ACHW4Z_003337"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR8882842.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="ACHW4Z_003338"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="ACHW4Z_003338"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR8882843.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="ACHW4Z_003339"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="ACHW4Z_003339"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR8882844.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="ACHW4Z_003340"
     CDS             11501..11653
                     /locus_tag="ACHW4Z_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8882845.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2833 a         2992 c         3238 g         2713 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggagt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggca gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac ctgccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatacgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacattcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcctggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaat
//
LOCUS       DAZSNG010000115        11682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZSNG010000115 DAZSNG010000000
VERSION     DAZSNG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.116"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..346
                     /locus_tag="ACHW4Z_003341"
     CDS             <1..346
                     /locus_tag="ACHW4Z_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8882846.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="ACHW4Z_003342"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="ACHW4Z_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR8882847.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="ACHW4Z_003343"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="ACHW4Z_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR8882848.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="ACHW4Z_003344"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="ACHW4Z_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8882849.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="ACHW4Z_003345"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="ACHW4Z_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR8882850.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="ACHW4Z_003346"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="ACHW4Z_003346"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR8882851.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="ACHW4Z_003347"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="ACHW4Z_003347"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR8882852.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="ACHW4Z_003348"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="ACHW4Z_003348"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR8882853.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="ACHW4Z_003349"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="ACHW4Z_003349"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR8882854.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="ACHW4Z_003350"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="ACHW4Z_003350"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR8882855.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="ACHW4Z_003351"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="ACHW4Z_003351"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR8882856.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="ACHW4Z_003352"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="ACHW4Z_003352"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR8882857.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /gene="yjfY"
                     /locus_tag="ACHW4Z_003353"
     CDS             complement(8961..9236)
                     /gene="yjfY"
                     /locus_tag="ACHW4Z_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR8882858.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="ACHW4Z_003354"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="ACHW4Z_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR8882859.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="ACHW4Z_003355"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="ACHW4Z_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR8882860.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="ACHW4Z_003356"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="ACHW4Z_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR8882861.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="ACHW4Z_003357"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="ACHW4Z_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR8882862.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="ACHW4Z_003358"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="ACHW4Z_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2871 c         3087 g         2957 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAZSNG010000116        11656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZSNG010000116 DAZSNG010000000
VERSION     DAZSNG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.117"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..309
                     /locus_tag="ACHW4Z_003359"
     CDS             <1..309
                     /locus_tag="ACHW4Z_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8882863.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
     gene            complement(508..858)
                     /locus_tag="ACHW4Z_003360"
                     /pseudo
     CDS             complement(508..858)
                     /locus_tag="ACHW4Z_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1061..2074)
                     /gene="intI1"
                     /locus_tag="ACHW4Z_003361"
     CDS             complement(1061..2074)
                     /gene="intI1"
                     /locus_tag="ACHW4Z_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8882864.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2232..2705
                     /gene="dfrA17"
                     /locus_tag="ACHW4Z_003362"
     CDS             2232..2705
                     /gene="dfrA17"
                     /locus_tag="ACHW4Z_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8882865.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2836..3624
                     /locus_tag="ACHW4Z_003363"
     CDS             2836..3624
                     /locus_tag="ACHW4Z_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR8882866.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3830..4177
                     /locus_tag="ACHW4Z_003364"
     CDS             3830..4177
                     /locus_tag="ACHW4Z_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8882867.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4171..5010
                     /gene="sul1"
                     /locus_tag="ACHW4Z_003365"
     CDS             4171..5010
                     /gene="sul1"
                     /locus_tag="ACHW4Z_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR8882868.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5138..5341
                     /locus_tag="ACHW4Z_003366"
     CDS             5138..5341
                     /locus_tag="ACHW4Z_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882869.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5497..6702
                     /gene="chrA"
                     /locus_tag="ACHW4Z_003367"
     CDS             5497..6702
                     /gene="chrA"
                     /locus_tag="ACHW4Z_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR8882870.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6713..7018
                     /locus_tag="ACHW4Z_003368"
     CDS             6713..7018
                     /locus_tag="ACHW4Z_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR8882871.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7245..8009
                     /locus_tag="ACHW4Z_003369"
     CDS             7245..8009
                     /locus_tag="ACHW4Z_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR8882872.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8502..9086)
                     /gene="mphR(A)"
                     /locus_tag="ACHW4Z_003370"
     CDS             complement(8502..9086)
                     /gene="mphR(A)"
                     /locus_tag="ACHW4Z_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR8882873.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9086..10324)
                     /gene="mrx(A)"
                     /locus_tag="ACHW4Z_003371"
                     /gene_synonym="mrx"
     CDS             complement(9086..10324)
                     /gene="mrx(A)"
                     /locus_tag="ACHW4Z_003371"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR8882874.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10321..11226)
                     /locus_tag="ACHW4Z_003372"
     CDS             complement(10321..11226)
                     /locus_tag="ACHW4Z_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR8882875.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11348..>11656)
                     /locus_tag="ACHW4Z_003373"
     CDS             complement(11348..>11656)
                     /locus_tag="ACHW4Z_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8882876.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         2451 a         3350 c         3409 g         2446 t
ORIGIN      
        1 cgattcatca acacggataa agcgcccgcc tatggtcgcg cgcttgctct gctcaaacgc
       61 gaaggccggt gcccgtctga cgttgaacac cgacagatta agtaccggaa caacgtgatt
      121 gaatgcgatc atggcaaact gaaacggata atcaacgcca cgctgggatt taaatccatg
      181 aagacggctt acgccaccat caaaggtatt gaggtgatgc gtgcactacg caaaggccag
      241 gcctcagcat tttattatgg tgatcccctg ggcgaaatgc gcctggtaag cagagttttt
      301 gaaatgtaag gcctttgaat aagacaaaag gctgcctcat cgctaacttt gcaacagtgc
      361 ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg tcagggaaga ctctatgacc
      421 ttcaacgaga tatgtcaata aattcaaaat tcaatcctat cctgacgcaa tttacacatg
      481 gcatctgaca tcaggttagg gtatgcctca acctgacggc ggcgaatcac aagcgtccgg
      541 tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg aagcgctgat agcactccag
      601 cccgcagaaa tgctccacgt actcggcccc ttccggcgtg aaggcggcat cgagcgggat
      661 ttccttgcag cacacgcagc aactggtgct cggttcattg gcgttcatgg tggtgttcct
      721 ccattggttg acgaagccga cgaaggccgc cgccggcatc ggcctggcga acaggaaacc
      781 ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg cagtcgggtg tttccacacc
      841 ctcggctacc acctccattg tcgttttcag aagacggctg cactgaacgt cagaagccga
      901 ctgcactata gcagcggagg ggttggatcc atcaggcaac gacgggctgc tgccggccat
      961 cagcggacgc agggaggact ttccgcaacc ggccgttcga tgcggcaccg atggccttcg
     1021 cgcaggggta gtgaatccgc caggattgac ttgcgctgcc ctacctctca ctagtgaggg
     1081 gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc aactttcagc acatgcgtgt
     1141 aaatcatcgt cgtagagacg tcggaatggc cgagcagatc ctgcacggtt cgaatgtcgt
     1201 aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag ggtgtgcggt gtggcgggct
     1261 tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg ctgaaaggtc tggtcataca
     1321 tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc tgcgcaaaaa
     1381 cccagaacca cggccaggaa tgcccggcgc gcggatactt ccgctcaagg gcgtcgggaa
     1441 gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca ccatgcccgt gcacgcgaca
     1501 gctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc
     1561 ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt
     1621 gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct
     1681 cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc
     1741 gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct
     1801 tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc
     1861 gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt
     1921 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1981 gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2041 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac
     2101 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2161 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttagccatta
     2221 agggagttaa attgaaaata tcattgattt ctgcagtgtc agaaaatggc gtaatcggta
     2281 gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact actctttaaa gcgctcacat
     2341 ataatcaatg gctccttgtc ggaagaaaaa catttgactc tatgggtgtt cttccaaatc
     2401 gcaaatatgc agtagtgtca aagaacggaa tttcaagctc aaatgaaaac gtcctagttt
     2461 ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt tacagatcat gtatatgtct
     2521 ctggcggggg tcaaatctat aatagcctta ttgaaaaagc agatataatt catttgtcta
     2581 ctgttcacgt tgaagtcgaa ggtgatatca aattccctat aatgcctgag aatttcaatt
     2641 tggtttttga acagtttttt atgtctaata taaattatac ataccagatt tggaaaaaag
     2701 gctaacaatg cgttgcagca ccagtcgctt cgctccttgg acagctttta agtcgcgtct
     2761 ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa cttaaaagct gccgctgaac
     2821 ttaacgttag gcatcatggg tgaatttttc cctgcacaag ttttcaagca gctgtcccac
     2881 gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg acacaatcca cctgttcgga
     2941 tctgcgatcg atggagggct gaagccggac agcgacatag acttgctcgt gaccgtcagc
     3001 gccgcaccta acgattcgct ccggcaggcg ctaatgctcg atttgctgaa agtctcatca
     3061 ccgccaggcg atggcggaac atggcgaccg ctggagctaa ctgttgtcgc tcgaagcgaa
     3121 gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt tcggtgagtg gctccgccac
     3181 gacatccttt ccggaacgtt cgagcctgcc gttctggatc acgatcttgc gattttgctg
     3241 accaaggcga ggcaacacag ccttgcgctt ctaggcccat ccgcagccac gtttttcgag
     3301 ccggtgccga aggagcattt ctccaaggcg cttttcgaca ctattgccca gtggaatgca
     3361 gagtcggatt ggaagggtga cgagcggaac gtcgttcttg ctcttgctcg catttggtac
     3421 agcgcttcaa ctggtctcat tgctcctaag gacgttgctg ccgcatgggt atcggagcgt
     3481 ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg cggcgtacct gggtagcgag
     3541 gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt tcgttcgata tgccaaagca
     3601 acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa gtgcatcgac ccgcgccgag
     3661 ggcatctgat gcctaactcg gcgttcaagc ggacgggctg cgcccgccgc tcaactatgc
     3721 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     3781 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg
     3841 gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag
     3901 cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt
     3961 ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc
     4021 gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga
     4081 tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc
     4141 atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc
     4201 accgaggact ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg
     4261 gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat
     4321 ccggacgcga ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac
     4381 gccctgtccg atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc
     4441 tatgcgctca agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg
     4501 ctctatcccg atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg
     4561 gatggcatcg ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg
     4621 cggttcttcg aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc
     4681 atcctcgatc cggggatggg atttttcttg agccccgcac cggaaacatc gctgcacgtg
     4741 ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg
     4801 cggaaatcct tcttgggcgc caccgttggc cttcctgtaa aggatctggg tccagcgagc
     4861 cttgcggcgg aacttcacgc gatcggcaat ggcgctgact acgtccgcac ccacgcgcct
     4921 ggagatctgc gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac
     4981 gccagagacc gagggttaga tcatgcctag cattcacctt ccggccgccc gctagcggac
     5041 cctggtcagg ttccgcgaag gtgggcgcag acatgctggg ctcgtcagga tcaaactgca
     5101 ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg gacagcgagg agcctccgaa
     5161 cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg cggctgatgc acgacgctgc
     5221 ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc gcgcggatcg accggacatt
     5281 cgcggagacc ttcgtcctga gatccgagct cctagggatc gcctcagaaa acggaaaata
     5341 aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc cacagctaaa actgcgtagt
     5401 acacatagag gtattttttt ctagatttat atcgaatttc gttatcgaat ttcgcttatt
     5461 attgatcaac tcgttgcaga tcatgaaggc gaaagtatga cgaataaccc taccgatgac
     5521 agcagaccat ggtcggtctt tcttattttt ctgcggcttg gattgacatc ttttggcggc
     5581 cccattgcgc acttgggcta cttccgcgcc gaatttgtca cacggcggcg ctggctctcc
     5641 gaacggagct atgctgactt ggtcgcgctt tgtcagttct tgccagggcc tgcaagcagc
     5701 caggtcggca tagcggtagg actgtctcgg gctggataca gcggggcgct ggctgcttgg
     5761 gctggcttca cgctgccgtc tgccatagcc ttgatccttt ttgcgctcgg catctccagc
     5821 tatggcgatt acgtctcgca gggcgcgttg catggcttaa aagtggtggc tgtggccgtg
     5881 gtcgctcaag cagtatgggg catggcgcgt aacctatgca cggatgggct gcgagtcacc
     5941 atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt ccgcgtgggg acaggttggc
     6001 gtgattgcta tcgcaggcat cgcaggccgg ttattgttca agccagcgaa agttgttgag
     6061 catgaccccc tacctatcac ggtcagtcac cgggccggcg tgctttggct ctcgctgttc
     6121 tttgtcttgc tgattggcct gccggtgttg gccgaactga tgccaagtca aaccatggca
     6181 atggtggatt ccttctatcg tgtcggatca ctggtgttcg gcggtggtca cgttgtgctg
     6241 ccattactgc aagccgaagt ggtgccctcc ggctgggtca acaatgaatc ctttctcgcg
     6301 gggtacgggg cagctcaagc ggtgcccggc cctttgttca cgttcgccgc gtttcttggt
     6361 gcctcgatga acaccgcccc gtcgggctgg atcggcggca ttgtgtgtct gctggctatc
     6421 ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat tttgggagcg tttgcgccgc
     6481 aatacaggca tccaagctgc gctggccggg atcaatgccg ctgtagtcgg cttgctgctg
     6541 gccgcgctgt atcagcctgt atggactagc gccatctttc agccgcaaga cttcggcttg
     6601 gcattagttg cccttgtcgc acttatgttc tggaagctcc cgccgtggct ggttgtcctc
     6661 ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt gaggcacaaa aaatgactga
     6721 caaagacctc tacggcggtt tgatccgcct gcacatcctt caccatgcag ccgaggaacc
     6781 tgtctttggg ctggggatca tcgaagagct acgccgacac ggctacgaga tgagcgctgg
     6841 caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc tatctgacct cacgccacga
     6901 acgcaccggg cgacgtgaac ggcgtgttta tgacatcact gaacaaggca gaactgcact
     6961 tgctgatgcg aaaacaaagg tcaaggagct gttcggagag ttggtagaag gtggttgagt
     7021 ttttgcttct atgtcaggtt gagctatacc ctaactggat tgtcattttc agaagacgac
     7081 tgcaccagtt gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca
     7141 gaagtgatct gcaccaatct cgactatgct caatactcgt gtgggctctg ttgcaaaaat
     7201 cgtgaagctt gagcatgctt ggcggagatt ggacggacgg aacgatgacg gatttcaagt
     7261 ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg ctggtattgt cgctatccga
     7321 tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg catttcggtc gaccatacga
     7381 cgatctatcg ctgggtccag tgctacgccc cggagatgga gaagcggctg cgctggttct
     7441 ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac ctacgtcaag gtgcggggca
     7501 agtggaccta cctgtaccgg gcagtcgaca agcggggcga cacgatcgat ttctacctgt
     7561 cgccgacccg cagcgccaag gcagcgaagc ggttcctggg caaggccctg cgaggcctga
     7621 agcactggga aaagcctgcc acgctcaata ccgacaaagc gccgagctat ggtgcagcga
     7681 tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac ggcccaccgg caggtgaagt
     7741 atctcaataa cgtgatcgag gccgatcacg gaaagctcaa gatactgatc aagccggtgc
     7801 gcggtttcaa atcgatcccc acggcctatg ccacgatcaa gggattcgaa gtcatgcgag
     7861 ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc
     7921 gccttgtgga gagagctttt ggcattgggc cctcggcgct gacggaggcc atgggcatgc
     7981 tcaaccacca tttcgcagca gccgcctgat cggcgcagag cgacagccta cctctgactg
     8041 ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct cggtgatggt ggagtgaagc
     8101 caacccgcaa tcgggttatg aatctgcatc gcgattcgca atcagctgtc tcttgagcat
     8161 gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc tacaccaact tgtccaactc
     8221 atccgccaca ccgctcccca ccacctgcga tggccaggtt gtttgctggg cgcattggcg
     8281 gagtatcgtc tcgacccggg acacccacac cggcacaacc ttacgggagt gattcactgt
     8341 caaagaatcg gcccggtgct ctgacgcaag tatcgggatg gtcaccattt gtaagccgta
     8401 gacctgagtg gtgatcaaga cttcgatacc accgaccgta ccggtactaa tcgacgacgg
     8461 tcgtattcgt cgcctgccgc agggactctg cacacctccg tttacgcatg tgcctggagg
     8521 agttggaaat cgtcgtgttc gggaaacatt aaacacagga tggcagcgat ctgagccagc
     8581 acatgatcag ctagctcacc atccggatcg acggcccact gcatcgtcgc gccagcgatg
     8641 accgagtgca ggagcaactc agctgccgca ggagcacctg ggggcagtcg cttgcggatc
     8701 ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc gtagctccgg cacctggagc
     8761 tcgtaccagg agatgagata gttcaccgag aagtcgttgc gagtgttcat gctccgaacg
     8821 agcacctgca aaaattccca gagcccttgc ggccctgcgc ctatcggtat cgcattcagg
     8881 taatgccgca cctgctcgac gccgcgctcc atcatcctca ccagcagcgt atcgcggttg
     8941 gtgaagcgct ggattaacgc tgcgcgggag agccccacct cctttgctac tccgctgagc
     9001 gtgaactcta tgggaccgca acgcttcagc actacggtgg cggcctcgag tacctcgtca
     9061 tcggacttga gcttggggcg gggcatcagt gttcaccttc tgtatgggtt ggggcggagg
     9121 ctgtggctgc cgccgccatt gtagcaaatt gaagacggag cgagagtaga gccacgagcc
     9181 ccgcaaacac ggccgataca acgaggccag ggagcggacc agcaaggtcg acaaacgcgc
     9241 cggccgcaag cataaccatg ggcgaggctg acagcatcac cgccgagacc gtgccgagta
     9301 cccggccgag aagttctggc ggcgtgcggt tgtagatggc agcgttgaga atgggagaga
     9361 ctgagccggt cagcagtccc acgagcgcgc ccaacaacat cagcaccggc acgcctggca
     9421 actgtgaaag cagaagcgag cccaccgcag agccacaaaa tgccaccgcc agccagttct
     9481 gcgctgatat ccgggcgccg accgacgcat gaatggcaat gccaaggaga ccaccagccc
     9541 ccatcattga ggagaacagc ccgagctctg ctacttggcg tcctgcatct acaaacagcg
     9601 caggcatgat gacgctgccg ttggcgccaa cgatgcccac gaagatcatc actataccaa
     9661 agagagggcg cagcaggggt tcgctccaga gaaaagcgac gccggcgcgc atggagagag
     9721 tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag cacccacgcg ccgagcagac
     9781 ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg cgcagcgcca agcaggccga
     9841 ttgcggcccc cccaagggcc gggccaccta gaatcgcgac gttcccgatc accgctttca
     9901 gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc gagtttgggc agctcactgt
     9961 cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag caccacgcaa gcgatcagcc
    10021 cagtgttcga gagggcgcca acggcatcga gcagtgggat gctcgccatg gccacgccgc
    10081 ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa ccgatcgacg aggccaccac
    10141 caacccacgc gccgatgatg gtcgcgatga cgctgctagc ggccgtggcg cccgcccagg
    10201 ccgcgctctt tgtatgagac aggacgaacc atggaagcgc gagggccgcc accgcgttgc
    10261 cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag cgggctatat cgacgttcgc
    10321 tcattccgct gcggcgagct gcgccttcgc cgcagcgagg tactcttcgt tacccgagtc
    10381 gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg cgctccgcga tgtggtgggc
    10441 gagccgcggc cacacccggc caccggccgc ttcatacgtg aggaggagct tcgcgagccc
    10501 ctcttcacca aagaccataa ggtgcgcggc catgtcgatg gcagggtcat caacgcgggc
    10561 ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg tcgatgagca catggcccac
    10621 gtagagatcg ccatgcacca ccacggagaa atctggccac gacgaatcgt cgtcgagcca
    10681 gcgctgccac cggtggaggc gcttgtcgtt caccacgaac tcgcgtcgga cgcggtcaac
    10741 gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg atgagcatcc ccgcatccac
    10801 ggcggcggaa atggggacgg catgcagggc ggcgagcgcg gtcgcgaagc tctccgcgaa
    10861 gacctccgag tcctgcggca cgacccagtc gggcgtggac gaaccaggct ggatgaccat
    10921 cgcagtcgag tcttcgagca tgggataggc aacgagctcg gcgttggcca cgcgccagtc
    10981 cggcaccgcg aacggcaggc gattcttgag cattgccagc acccgcgcct ctggttcgac
    11041 cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc caccgacgtc catcgtcgac
    11101 ggtggcgatc acgatcctat agtcgagccc aagctcattg acagtcagcg ggccatggag
    11161 cttgagccca tgtcgggctg caagtgcgta cagttgggag gtatcggcgg tcgtgactac
    11221 ggtcatgatt cactcctgag ggcttgacgg gtttagccac ctaaatgtaa cagtcacgtc
    11281 ggttatattc aatccggcac tgttgcaaag ttagcgatga ggcagccttt tgtcttattc
    11341 aaaggcctta catttcaaaa actctgctta ccaggcgcat ttcgcccagg ggatcaccat
    11401 aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat cacctcaata cctttgatgg
    11461 tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc gccgattatc cgtttcagtt
    11521 tgccatgatc gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt tcaacgtcag
    11581 acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg accataggcg ggcgctttat
    11641 ccgtgttgat gaatcg
//
LOCUS       DAZSNG010000117        11655 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZSNG010000117 DAZSNG010000000
VERSION     DAZSNG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.118"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..794
                     /locus_tag="ACHW4Z_003374"
     CDS             <1..794
                     /locus_tag="ACHW4Z_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR8882877.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHW4Z_003375"
     CDS             complement(744..1937)
                     /locus_tag="ACHW4Z_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8882878.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHW4Z_003376"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHW4Z_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR8882879.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHW4Z_003377"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHW4Z_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR8882880.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHW4Z_003378"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHW4Z_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR8882881.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHW4Z_003379"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHW4Z_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR8882882.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHW4Z_003380"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHW4Z_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR8882883.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHW4Z_003381"
     CDS             complement(10573..10716)
                     /locus_tag="ACHW4Z_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882884.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="ACHW4Z_003382"
     CDS             10955..>11655
                     /locus_tag="ACHW4Z_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR8882885.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAZSNG010000118        11521 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZSNG010000118 DAZSNG010000000
VERSION     DAZSNG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.119"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1046)
                     /locus_tag="ACHW4Z_003383"
     CDS             complement(<1..1046)
                     /locus_tag="ACHW4Z_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8882886.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1626
                     /locus_tag="ACHW4Z_003384"
                     /pseudo
     CDS             1537..1626
                     /locus_tag="ACHW4Z_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            1928..2923
                     /gene="pgl"
                     /locus_tag="ACHW4Z_003385"
     CDS             1928..2923
                     /gene="pgl"
                     /locus_tag="ACHW4Z_003385"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR8882887.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2964..3917)
                     /locus_tag="ACHW4Z_003386"
                     /pseudo
     CDS             complement(2964..3917)
                     /locus_tag="ACHW4Z_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4102..5154
                     /locus_tag="ACHW4Z_003387"
     CDS             4102..5154
                     /locus_tag="ACHW4Z_003387"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR8882888.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            5230..6663
                     /locus_tag="ACHW4Z_003388"
     CDS             5230..6663
                     /locus_tag="ACHW4Z_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR8882889.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6846..9026
                     /locus_tag="ACHW4Z_003389"
     CDS             6846..9026
                     /locus_tag="ACHW4Z_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR8882890.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(9167..10450)
                     /locus_tag="ACHW4Z_003390"
     CDS             complement(9167..10450)
                     /locus_tag="ACHW4Z_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR8882891.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(10585..>11521)
                     /locus_tag="ACHW4Z_003391"
     CDS             complement(10585..>11521)
                     /locus_tag="ACHW4Z_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8882892.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2873 a         2585 c         2861 g         3202 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg tagcgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaatg
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tagtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tcaaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga ttgaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt ttatttgtgc cggaagtgag
     1621 ttgattgttg cgcgaatggg aagttggcca gaagttgtta tggcatagtg tttacggtcg
     1681 ttgatacagg taaacaaaag cggggagagt catactccct gaatagtcgc gaaaatatct
     1741 gtggcattgt caaattcgtg tcggtgacta atattttagt aatatgccat tcgaaatgga
     1801 gtgctaactt attgatttaa ttgatgtgcg atattgtatt ttatcgtgtt ttgttgtctg
     1861 ttttttatcc agtaagtgtt gttatatgta tgaaagataa gggatttatc atttttgagg
     1921 tgtgttcatg aagcaaacag tttatatcgc cagccctgag agccagcaaa ttcacgtctg
     1981 gaacctgaat catgaaggcg cactgacgct gacacaggtt gtcgatgtgc cggggcaggt
     2041 gcagccgatg gtggtcagcc cggacaaacg ttatctctat gttggtgttc gccctgagtt
     2101 tcgcgtcctg gcgtatcgta ttgccccgga cgatggcgca ctgacctttg ccgcagagtc
     2161 tgtgctgccg ggtagtccga cgcatatttc caccgatcac caggggcagt ttgtctttgt
     2221 aggttcttac aatgcgggta acgtgagcgt aacgcgtctg gaagatggcc tgccagtggg
     2281 cgtcgtcgat gtagtcgagg ggctggacgg ttgccattcc gccaatatct caccggacaa
     2341 ccgtacgctg tgggttccgg cattaaagca ggatcgcatt tgcctgttta cggtcagcga
     2401 tgatggtcat ctggtggcgc aggaccctgc ggaagtgact accgttgaag gggccggccc
     2461 gcgtcatatg gtattccatc caaacgaaca atatgcgtat tgcgtcaatg agttaaacag
     2521 ctcagtggat gtctgggaac tgaaagatcc gcacggcaat atcgaatgtg tccagacgct
     2581 ggatatgatg ccggaaaact tctccgacac ccgttgggcg gctgatattc atatcacccc
     2641 ggatggtcgc catttatacg cctgcgaccg taccgccagt ctgattaccg ttttcagcgt
     2701 ttcggaagat ggcagcgtgt tgagtaaaga aggcttccag ccaacggaaa cccagccgcg
     2761 cggcttcaat gttgattaca gcggcaagta tctgattgcc gctgggcaaa aatctcacca
     2821 catctcggta tacgaaattg ttggcgagca ggggctactg catgaaaaag gccgctatgc
     2881 ggtcgggcag ggaccaatgt gggtggtggt taacgcacac taaccgctga tttaaccggc
     2941 gcagtctctc ctgcgccggt gtattaactt atctcctgta acgcatgtct ctggcgttcg
     3001 acgatattgg tccacaaatt gtctttatcg tcagtccaca aattaatcag caaggaaaaa
     3061 aagcgttcag ctgccggaga aagcacggca tctttgcggc gaataatccc caatgtccga
     3121 cgtataacgg gttccaccag tgggatacca ataatcgaag aatagggcgc atggggcatt
     3181 gccagaccag gaagcgccga aatccccagt cctgcctcca ctagtcctaa tgacgtcgaa
     3241 agatgacgca cttcgtaaaa ccagtccagc ttccagggct tgtcggctag ctgttgttct
     3301 atcaacaatc ggttgccgct ggaggaacgt acgccaatca ttttgtaacc cactaactcc
     3361 tgccattcta caagctactt tttggccaat ggatggtcac gccgacaggc cagcacgaat
     3421 ggctcgttga ccagtggggt aaaatcaatg gatgaatttg tgacgttgtt catgtttatg
     3481 ccaaagtctg attcgttaca cagcaccgat tccatgcaat tattcgtgcc ttgttccaga
     3541 atccgcactt taatattggg atacagctca ttaaatttac cgattgccag cggtaaaaaa
     3601 taaaatactg cggtcggaat acacgctaat gtcaccatac cacgatgata agcattcata
     3661 tcacgaatat taaaaagcgt ttcatcgaat ttctttatta attcccgcgc ctccgggagc
     3721 aacctttttc ctgcttttgt taacgtaacc ttgcgagttg tacgctcaaa tagttgaata
     3781 tgtaaatctt cctccatctt ttttattctt cgcgttaatg caggttgcgt aatattgagt
     3841 aatttagcgg cattgttaaa tgaactggac tctgccagta tgacaaatgc cttcatactt
     3901 gataattcat gcttcattat tactccggaa aatggaagcg acgattttgg gggggctggt
     3961 cgttaaaaat tttaactgca tttagccaac ttaaattaat gaaaaaatgt tattaatcgt
     4021 tgagctaaag tcattagaga tgctttgtcc ttaatgtaac catatcgcaa taagttatgt
     4081 ttttaaattg agggcattat tatgaaaaaa ataccctgcg tgatgatgcg aggtggaacc
     4141 tcgaggggcg cgttcctgtt agcggaacat ttacccgaag atcaaacgca gcgcgataaa
     4201 atattgatgg caattatggg ttccggtaac gatctggaaa ttgacggtat tggcggcggt
     4261 aatccactga ccagtaaagt cgccattatt agccgttcca gcgatccgcg tgctgatgtc
     4321 gattatctgt ttgctcaagt tatcgtccat gagcaacgtg tcgataccac gcctaactgc
     4381 ggcaatatgc tgtctggcgt aggggcattc gccattgaaa atggtttgat tgcagcgact
     4441 tcgccagtta cccgtgtacg tatccgcaac gtcaatacgg gtacgttcat cgaagctgat
     4501 gtgcaaacgc caaatggtgt tgtcgagtac gagggtagcg ccagaattga cggcgtaccg
     4561 ggtactgccg caccggttgc gctcactttc ctgaatgccg ctggaacgaa aactggaaaa
     4621 gttttcccga ctgataatca gattgattat tttgacgatg tcccggtgac ctgtatcgat
     4681 atggcgatgc cagtcgtcat tattccggct gaatatctgg gaaaaacagg ttatgaatta
     4741 ccggcggaac tggatgccga caaagcatta ttagcccgca ttgaatctat ccggctacaa
     4801 gcgggtaaag caatgggctt aggtgatgtc agtaatatgg ttatccctaa acctgtgctt
     4861 atttctccag cgcagaaagg cggggcaatt aatgtgcgtt attttatgcc gcattcttgc
     4921 catcgcgcgc tggcgataac cggtgctatt gctatttcca gtagttgtgc attggaaggc
     4981 accgtcaccc gacaaatcgt cccttctgta ggatatggca atatcaatat tgaacacccc
     5041 agtggtgcgc tcgacgttca tttaagtaat gaaggtcagg atgccacgac gttacgcgca
     5101 tctgttattc ggacaaccag aaaaatattt tccggtgaag tttatcttcc ctgaaaaaat
     5161 tcgttgtcag gataaggaca atcaataaag gacttctgta tgagtcatac agaaagaaca
     5221 ggattttaaa tgaataagaa atcgttatgg aagctaattc tgatattagc gatcccatgt
     5281 attattggtt ttatgccagc tccggcagga ttaagcgaac tggcgtgggt gctttttggt
     5341 atttacctgg cggccattgt gggtctggtt atcaagcctt tcccggaacc tgtcgtactg
     5401 ttaattgccg ttgctgcctc aatggtggtg gtcggtaact tatccgacgg tgcgtttaag
     5461 accaccgccg tattaagcgg ttactcttca ggtactacct ggctggtgtt ctcggcgttt
     5521 accttaagcg ccgcatttgt gaccaccggt ttaggtaaac ttattgccta tctgctgatt
     5581 ggtaaaatcg gtaacaccat gctgggtctg ggttacgtta cggtattcct cgatctggta
     5641 ctggctccgg caacaccgtc taacaccgcg cgtgcgggcg gcattgtgtt accgatcatc
     5701 aacagcgtgg cggtggcttt ggggtccgaa ccggaaaaaa gtccgcgtcg tgtcggacat
     5761 tacctgatga tgtccattta catggtcacc aaaaccacca gctatatgtt ctttaccgca
     5821 atggcgggga acattctggc gctgaaaatg atcaacgaca ttctgcacct gcaaattagc
     5881 tggggtggat gggcgctggc cgccggattg ccgggcatca ttatgctgct ggtcaccccg
     5941 ctggtaattt acaccatgta tccaccagaa attaaaaagg tggataacaa aaccatcgct
     6001 aaagcgggcc ttgccgaact gggaccgatg aaaatccgcg aaaaaatgct gctcggtgtc
     6061 tttgtgctgg cgctactggg ctggattttc agtaagtctc tgggggttga tgaatccact
     6121 gtggcaatcg ttgttatggc gaccatgctg ctgctgggta tcgttacctg ggaagacgtg
     6181 gttaaaaata aaggcggctg gaatacctta atctggtacg gcggtattat cggcttaagc
     6241 tccttattat cgaaagttaa atttttcgaa tggttagctg aagtctttaa aaataacctg
     6301 gcatttgatg gtcacggtaa cgttgctttc ttcgttatta ttttcctcag catcatcgtg
     6361 cgttatttct tcgcttccgg tagtgcctat atcgttgcta tgttacctgt atttgccatg
     6421 ctggcgaacg tctccggcgc accgttaatg ttaaccgcgc tggcactgtt gttctccaac
     6481 tcctatggcg gcatggttac tcactatggc ggcgcggcag gtccggtcat ctttggcgtg
     6541 ggttacaacg atattaaatc ctggtggttg gtcggtgcgg tactgacgat attaaccttc
     6601 ttggtgcata tcaccctcgg cgtgtggtgg tggaatatgc tgatcggctg gaacatgctg
     6661 taaatatact cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc
     6721 acttacttat gtaagctcct ggggattcac tcacttgtcg ccttcctgca actcgaatta
     6781 tttagagtat atccatttat tatctttctg cgcacttcac ggtgcgcaga tatctggagc
     6841 atttgatgat caagttatct gaaaaaggcg tgtttctcgc cagtaataac gaaataattg
     6901 ccgaagaaca tttcaccggc gaaattaaaa aagaagaagc aaaaaaaggc actattgcct
     6961 ggtctattct ctcttctcat aatacgtccg gaaatatgga taaacttaaa attaagtttg
     7021 attcattagc ctctcacgat attacctttg ttggtattgt acagaccgct aaagcgtccg
     7081 gaatggaacg tttcccgctg ccgtatgtgc tgaccaactg ccataactca ctctgcgccg
     7141 tcggcggcac cattaacggt gatgaccatg tttttggttt atcggcagct cagcgttatg
     7201 gcggtatttt tgtgcctccg catattgcgg tcatccatca atatatgcgt gagatgatgg
     7261 ctggcggggg caaaatgatc ctcgggtcag acagccacac ccgttacggt gcattaggga
     7321 caatggcagt cggtgagggt ggcggtgagt tggtaaaaca gctgcttaat gacacctggg
     7381 atatcgacta tccgggcgtg gttgcggtgc atctgaccgg aaaacctgcg ccgtatgtgg
     7441 ggccacagga tgtggcgctg gctatcattg gcgcggtgtt caaaaacggt tacgtcaaaa
     7501 acaaagtcat ggagttcgtt ggaccgggcg ttagcgcgct ctctaccgat ttccgtaaca
     7561 gcgttgacgt gatgaccacg gaaaccacct gtttaagctc cgtctggcaa actgatgaag
     7621 aagtgcataa ctggctggcg ctgcacggtc gcggtcagga ttactgccag cttaaccctc
     7681 aaccgatggc gtactacgat ggctgcatca gcgttgattt aagcgccatc aaaccaatga
     7741 ttgcgctgcc gttccacccg agcaacgtgt atgaaatcga cacactgaac cagaacctga
     7801 ccgacattct gcgtgagatt gaaattgagt ccgaacgcgt ggcgcacggt aaagccaaac
     7861 tctcgctgct ggataaagtg gaaaatggtc gcctgaaagt gcagcagggg attatcgcgg
     7921 gctgttctgg cggtaactac gaaaacgtca tcgcggcggc gaatgcactg cgcggtcaat
     7981 cctgtggcaa tgacaccttc tcgctggcgg tttacccgtc atcacagccg gtgtttatgg
     8041 atctcgccaa aaaaggtgtg gtagcagatt tgattggcgc aggcgcaatc atcagaaccg
     8101 cattctgtgg cccatgcttt ggcgcgggcg atacgccaat caacaacggt ttgagtattc
     8161 gccacaccac gcgtaacttc ccgaaccgcg aaggctctaa gccagccaat gggcagatgt
     8221 cagcggtggc gttgatggac gctcgttcta tcgctgcgac tgcggcaaac ggtggctatt
     8281 taacctctgc cagcgaactt gattgctggg acaacgtgcc ggagtacgcc ttcgatgtaa
     8341 cgccgtataa aaaccgtgtt tatcagggct ttgtgaaagg ggcaactcag caaccgctga
     8401 tttacggacc gaacattaaa gactggccgg aattgggtgc gctgactgac aatatcgtcc
     8461 tgaaagtgtg ctcgaagatc ctcgacgaag tcaccaccac cgacgaactg attccttccg
     8521 gtgaaacctc ttcttatcgt tcaaatccga ttggtctggc ggggaatgtc agcgagctga
     8581 cagaggtgtt tgcgcgcatt aagcagattg ctggtcagga gcatattgat ccgctgcaaa
     8641 ctgaaattgg cagcatggtc tatgcggtga aaccaggcga tggttctgcg cgtgaacagg
     8701 cggcgagctg ccagcgtgtg attggcggtc tggcgaatat tgccgaggag tacgcgacta
     8761 aacgctaccg ttctaacgtc atcaactggg ggatgttacc gctgcagatg gcggaagtgc
     8821 caacctttga agtgggggat tacatttaca tccctggcat taaagcggcg ctggataatc
     8881 cgggtacgac gtttaaaggt tatgtgatcc atgaagatgc gccggtaacg gaaattacgc
     8941 tttacatgga aagcctgacg gctgaagagc gcgagattat caaggcgggt agtttgatta
     9001 acttcaataa aaaccgtcag atgtaaaaag cgccatgtga atgtaggtcg cattcggcac
     9061 ttattgtcgg atgcgatgct ggcgcatctt atccgaccta caaatcgcat cgaaccgtag
     9121 gccggataag gcgtttacgc cgcatccggc aaatagttaa tagctcttac ttcttctcct
     9181 ctgcaaccac tttgctgccc acgccgcggt tattgtattc ccacatgcgg ttgtagttag
     9241 tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta ttacccgcaa
     9301 acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa ccttcgttga
     9361 tggcgctatc acggatcacc acctgaccgt tggtattagc atcaacatcc agcgagcggc
     9421 ccagttgcgc cacgccatca ccggaagcat tgaaacggct gtttacggcg aggaaaccgt
     9481 aatagatgtt agacagcgta gccggtgcaa acacatatgc ttcttgctgg gtacgtgagt
     9541 tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa acgatatcca
     9601 catccccttc aatatagctg ttggtcacca gcgtacgcgg ctgacggttg gtttccagac
     9661 ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga atgttaacgt
     9721 tgttaatctg tacttggtca ccgtcagtac gcagtgctac cgccggatgg ttacctgcat
     9781 ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagt ccattgtttt
     9841 gtgaccagaa gaccgcagaa cacagaacac cgatactgtc gctgcgtttg ctctggcagc
     9901 tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg ttgacgtcgt
     9961 gacgccagtc ggcagggctc atgcccccat caagggaaag cccaatcttc acatcaatcg
    10021 gtttttcacc tgtaccgtac agagtgattc cacctggagc agcagggaca tacaccgttc
    10081 cctgatactc accaggcatc acggcaatat actggcgctt gttggtgcgc ttgataattg
    10141 ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtaccc gccgggccga
    10201 caacaaagtc aggttgcgca ggcagggtta tcggggacgg attccacgct gcagcacctg
    10261 gtgtcaggga tgcaaaatag tgttgcgcat cgaaattcag cgcttctttt gccgacagaa
    10321 tcgggcgaga agaggtacca ggcgcagttt gatcagaagg acgttgatcg ggtggtgttg
    10381 agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg gaaactgaaa
    10441 atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg aagcctgctt
    10501 ttttatacta agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
    10561 tcattatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact ccctgcctct
    10621 gtcatcacga tactgtgatg ccatggtgtc cgacttgtgc ccgagaagat gttgagcaaa
    10681 cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact cgtgaaaggt
    10741 aggtggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa aataccttga
    10801 tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt ctccgccaag
    10861 aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat caacatgcaa
    10921 tgttgtaggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa gatatccatc
    10981 tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg taacaacagc
    11041 cagttccatt gcaagtctga gccaacatgg tgatgattct gctgcttgat aaattttcag
    11101 gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc gagtggcagc
    11161 gaccgggttt gttgttatat ggccttcagc tattgcctct cggaatgcat cgctcagtgt
    11221 tgatctgatt aacttggctg atgccgcctt gccctcgtct atgtatccat tgagcattgc
    11281 cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc tccttattgc
    11341 tttaattttg ctcatgtaat ttatgagtgt cttctgcttg attcctctgc tggcgaggat
    11401 tttttcgtag cgatcaagcc atgaatgtaa cgtaacagaa ttatcactgt tgattctcgc
    11461 tgtcagaggc ttgtgtttgt gtcctgaaaa taactcaatg ttggcctgta tagcttcagt
    11521 g
//
LOCUS       DAZSNG010000119        11512 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZSNG010000119 DAZSNG010000000
VERSION     DAZSNG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.120"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            197..1338
                     /gene="yghO"
                     /locus_tag="ACHW4Z_003392"
                     /pseudo
     CDS             197..1338
                     /gene="yghO"
                     /locus_tag="ACHW4Z_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1462..2232)
                     /gene="glcC"
                     /locus_tag="ACHW4Z_003393"
     CDS             complement(1462..2232)
                     /gene="glcC"
                     /locus_tag="ACHW4Z_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR8882893.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2483..3982
                     /gene="glcD"
                     /locus_tag="ACHW4Z_003394"
                     /pseudo
     CDS             2483..3982
                     /gene="glcD"
                     /locus_tag="ACHW4Z_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3982..5034
                     /gene="glcE"
                     /locus_tag="ACHW4Z_003395"
     CDS             3982..5034
                     /gene="glcE"
                     /locus_tag="ACHW4Z_003395"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR8882894.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5045..6264
                     /gene="glcF"
                     /locus_tag="ACHW4Z_003396"
                     /pseudo
     CDS             5045..6264
                     /gene="glcF"
                     /locus_tag="ACHW4Z_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6269..6673
                     /locus_tag="ACHW4Z_003397"
     CDS             6269..6673
                     /locus_tag="ACHW4Z_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HFR8882895.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6695..8866
                     /gene="glcB"
                     /locus_tag="ACHW4Z_003398"
     CDS             6695..8866
                     /gene="glcB"
                     /locus_tag="ACHW4Z_003398"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR8882896.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9221..10807
                     /locus_tag="ACHW4Z_003399"
     CDS             9221..10807
                     /locus_tag="ACHW4Z_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR8882897.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2731 a         2816 c         3167 g         2798 t
ORIGIN      
        1 ttaagaagaa gccttgtccg gtcgccttaa attgacaaaa ggcattgagg cgtttctgtg
       61 tagcctctga ccttatcata atcattaatt aatactattt attcgtcgtt tgtattcatt
      121 gagttgatta ttaagatgcc gctcgttgct gttgcaacct gtttttcacc attaattatt
      181 tgttatccac agagatatgg aatgcgatct gctgatgatt aaaattgaaa aagtaattaa
      241 taaaaatgac cttaaggcat ttatcgcttt tccgtcatca ctttatcccg acgatccaaa
      301 ctggataccc cctttattca ttgaacgcaa cgagcatttg tctgcgaaaa atccggggac
      361 ggaccatatc atctggcagg cgtgggtggc aaaaaaagcg gggcagatag ttgggcgtat
      421 taccgcgcaa atagatacct tgcaccgtga gcgttacggt gaagataccg gtcatttcgg
      481 catgattgac gccattgatg atccgcagga ttttgccgcg ttgtttggcg cagcggaagc
      541 gtggttgaag tcacaaggtg caagtaagat cagcgggcct ttcagcctga atatcaatca
      601 ggaaagcgga ttactgattg aaggttttga cacaccaccc tgtgcgatga tgccacacgg
      661 caaaccgtgg tatgccgcgc atattgaaca actgggttat cacaaaggca ttgatttact
      721 ggcgtggtgg atgcagcgaa ccgatctcac tttctctccg gcgctaaaaa aactgatgga
      781 tcaggtgcgc aaaaaggtga ccattcgctg catcaatcgt cagcggtttg ccgaagagat
      841 gcagatcctg cgtgagattt tcaactctgg ctggcagcac aactggggat ttgtgccgtt
      901 taccgaacat gaattcgcga ccatgggcga ccaacttaaa tatctggtgc cggacgatat
      961 gatctacatc gctgagattg attctgcacc ctgcgcgttt attgtggctt gcctaacatc
     1021 aacgaggcga ttgccgatct gaacggatcg ctctttccct tcggctgggc aaaattgctg
     1081 tggcgtttga aagtcagcgg tgtgcgaact gcgcgagtac cgctgatggg cgtgcgcgac
     1141 gagtatcagt tcagccgcat cggcccggtg attgcgctgt tattgattga agccttacgc
     1201 gatccgtttg cccgccggaa gatcgatgcg ctggagatgt catggatcct cgaaaccaat
     1261 acaggcatga ataacatgct ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg
     1321 tatgaaaagc aaatttaata ccagatcctg tttagcgcag gggcggtcat tcgggagagg
     1381 gtatagatga acagatttaa caccagatcc tgttaagtgc aggggcggtc ccctcgcctt
     1441 ggggataggg taaaactctg ctcacattcc caccttgttc atctccagcg gcaccgccga
     1501 gcgaatcaaa tggtggcctt ccagctcgat atcgtggaga ttctttttca cggtccgtac
     1561 atgatcgcgc gctgcgcgct gagcgacgtg cggcagccgc tgcaacaccg cgttgtagat
     1621 ccgcgcatgc tggcgatcga tctgcttttt ttgctgtggt cgatggtaga gattatttac
     1681 gctggcaaac actgaattaa acatcagatc ggtcaatgat tgcagcgtaa acaccagcac
     1741 ctgattgtga gaagcctgac aaatggcgag atggaaagcg tgatccaact gcgcatgttc
     1801 gatcagcgaa atctctttgt ggttctcact ggcggcgagc attttttcat aacagcgggt
     1861 tatcacaaca aaatcagcct gcgttcccag cgttgccgcc agccttgccg attcgccctc
     1921 cagtaatgcg cgaacgtcga gcagatcgta cagcgttcgc ggctgcgtac tgaacagatg
     1981 gatcagcggg ctggtgtcct gctcccgatt aagccgtgcg acatgagaat cgcgaccctg
     2041 cgccgtttca ataatcccgc gcccgcgcag cacggtcagc ccttcacgca gtgcggagcg
     2101 tgagaagccg agcttttcac acagtcgacg ttccgaggga agcggctgac cgaccttcag
     2161 tacgccgtcg ataattaacc gttcgatact ctctgcaacc acttcgcaaa tagggcgacg
     2221 ttcatctttc attcccggac ctcgtgcaca ggtaggacca atttttctgt tcttaactcg
     2281 caaaacacgc acatcacgca agtgattatg ttacatcaat ttaacattaa gttaaccaag
     2341 acaaggtcac agagctggaa aaaaaatggt ctgaccggta gctaaagaga tagacgaaaa
     2401 cgaaaagccc gcttaataac tgttcacaga agcagcgcgc aaaaatcagc tgccacacaa
     2461 cacaacaaag cgaagcctac tcatgagcat cttgtacgaa gagcgtcttg atggcgcttt
     2521 acccgatgtc gaccgcacat cggtactgat ggcactgcgt gagcatgtcc ctggacttga
     2581 gatcctgcat accgatgagg agatcattcc ttacgagtgt gacgggttga gcgcgtatcg
     2641 cacgcgtcca ttactggttg ttctgcctaa gcaaatggaa caggtgacag cgattctggc
     2701 tgtctgccat cgcctgcgtg taccggtggt gacccgtggt gcaggcaccg ggctttctgg
     2761 tggcgcgctg ccgctggaaa aaggtgtgtt gttggtgatg gcgcgcttta aagagagcct
     2821 cgacattaac cccgttggtc gccgcgcgcg cgtgcagccg ggcgtatgta acctggcgat
     2881 ctcctaggcc gttgcgccgc ataatctcta ttacgcgccg gatccttcct cacaaatcgc
     2941 ctgttcaatt ggcggcaatg tggcggaaaa tgccggtggc gtccactgcc tgaaatatgg
     3001 tctgaccgta cataacctgc tgaaaattga agtgcaaacg ctggacggcg aggcgctgac
     3061 gctgggatcg gacgcgctgg attcacctgg ttttgacctg ctggcgctgt tcaccggatc
     3121 ggaaggtatg ctcggcgtga ccaccgaagt gacggtaaaa ctgctgccga agccgcccgt
     3181 ggcgcgggtt ctgttagcca gctttgactc ggtagaaaaa gccggacttg cggttggtga
     3241 catcatcgcc aatggcatta tccccggcgg gctggagatg atggataacc tgtcgatccg
     3301 cgcggcggaa gattttattc atgccggtta tcccgtcgac gccgaagcga ttttgttatg
     3361 cgaactggac ggcgtggagt ctgacgtaca ggaagactgc gagcgggtta actacatctt
     3421 gttgaaagcg ggcgcgactg acgtccgttt ggcacaggac gaagcagagc gcgtacgttt
     3481 ctgggccggt ctcaaaaatg cgttcccggc ggtaggacgt atctccccgg attactactg
     3541 catggatggc accatcccgc gtcgcgccct gcctggcgta ctggaaggca ttgcccgttt
     3601 atcgcagcaa tatgatttac gtgttgccaa cgtctttcat gccggagatg gcaacatgca
     3661 cccgttaatc cttttcgatg ccaacgaacc tggtgaattt gcccgcgcgg aagagctggg
     3721 cgggaagatc ctcgaactct gcgttgaagt tggcggcagc atcagtgggg aacatggcat
     3781 cgggcgagaa aaaatcaatc aaatgtgcgc ccagttcaac agcgatgaaa tcacgacctt
     3841 ccatgcggtc aaggcggcgt ttgaccccga tggtttgctg aaccctggga aaaacattcc
     3901 cacgctacac cgctgtgctg aatttggtgc catgcatgtg catcacggtc atttaccttt
     3961 tcctgaactg gagcgctttt gatgctacgc gagtgtgatt acagtcaggc gctgctggag
     4021 caggtgaatc aggcaattag cgataaaacg ccgctggtga ttcagggcag caatagcaaa
     4081 gcctttttag gtcgcccagt caccgggcaa acgctggatg tgcgttgtca tcgcggcatt
     4141 gttaattacg acccgaccga gctggtgata accgctcgtg ccggaacgcc gctggtggcg
     4201 attgaagcgg cgctggagag cgccgggcaa atgcttccct gcgagccgcc gcattatggt
     4261 gaagaagcca cctggggcgg gatggtcgcc tgcgggctgg cggggccgcg tcgcccgtgg
     4321 agcggttcgg tccgcgattt tgtcctcggc acgcgcatca ttaccggcac tggaaaacat
     4381 ctgcgttttg gtggcgaagt gatgaaaaac gttgccggat acgatctctc acggttaatg
     4441 gccggaagct acggttgtct tggcgtgctc actgaaatct caatgaaagt gttaccgcga
     4501 ccgcgtgcct ctctgagcct gcgtcgggaa atcagcctgc aagaagccat gagtgaaatc
     4561 gccgagtggc aactccagcc attacccatt agtggcttat gttacttcga caatgcgttg
     4621 tggatccgcc ttgagggcgg cgaaggatcg gtaaaagcag cgcgtgaact gctgggtggc
     4681 gaagaggttg tgggtcagtt ctggcagcaa ttgcgtgaac aacaactgcc gttcttctcg
     4741 ttaccaggta ccttatggcg catttcatta cccagcgatg cgccgatgat ggatttaccc
     4801 ggcgagcaac tgatcgactg gggcggggcg ttacgctggc tgaaatcgac agccgatgac
     4861 aatcaaatcc atcgcatcgc ccgcaacgct ggcggtcatg cgacccgctt tagtgccgga
     4921 gatggtggct ttgccccgct atcggctcct ttattccgct atcaccagca gcttaaacag
     4981 cagctcgacc cttgcggcgt gtttaacccc ggtcgcatgt acgcggaact ttgaggagca
     5041 ggctatgcaa acccgattaa ccgaagagat gcggcagaac gcgcgctgga agccgacagc
     5101 atcctgtgcg cctgtgttca ctgcggattt tgtaccgcaa cctgcccaac ctatcagctt
     5161 ctgggcgatg aactggacgg gccgcgcggg cgcatctatc tgattaaaca ggtgctggaa
     5221 ggcaacgaag tcacgcttaa aacacaggag catctcgatc gctgcctcac ttgccgcaat
     5281 tgtgaaacca cctgtccttc tggtgtgcgc tatcacaatt tgctggatat cgggcgtgat
     5341 attgtcgagc agaaagtgaa acgcccactg ccggagcgaa tgctgcgcga aggattgcgc
     5401 caggtagtgc cgcgtccggc ggtcttccgt gcgctgacgc aggtagggct ggtgctgcga
     5461 ccgtttttac cggaacaggt cagagcaaaa ctgcctgctg aaacggtgaa agctaaaccg
     5521 cgtccgccgc tgcgccataa gcgtcgggtt ttaatgctgg aaggctgcgc ccagcctacg
     5581 ctttcgccca acaccaacgc ggcaactgcg cgagtgctgg atcgtctggg gatcagcgtc
     5641 atgtcagcta acgaagcagg ctgttgtggc gcggtggact atcatcttaa tgcgcaggag
     5701 aaagggttgg cacgggcgcg caataatatt gatgcctggt ggcccgcgat tgaagcaggt
     5761 gccgaggcaa ttttgcaaac cgccagcggt tgcggcgcgt tcgtcaaaga gtatgggcag
     5821 atgctgaaaa acgatgcgtt atatgccgat aaagcgcgtc aggtcagtga actggcggtc
     5881 gatttagtcg aacttctgcg cgaggaaccg ctggaaaaac tggcaattcg cggcgataaa
     5941 aaactggcct tccactgccc gtgtacccta caacatgcgc aaaagctgaa cggcgaagtg
     6001 gaaaaagtgt tacttcgtct tggatttacc ttaacggacg ttcccgacag ccatctgtgc
     6061 tgcggttcag cgggaacata tgcgttaacg catcccgatc tggcacgcca gctgcgggat
     6121 aacaaaatga atgcgctgga aagcggcaaa ccggaaatga tcgtcaccgc caacattggt
     6181 tgccagacgc atctggcgag cgccggtcgt acctctgtgc gtcactggat tgaaattgta
     6241 gaacaagccc ttgaaaagga ataaaaaaat gaaaactaaa gtcattctta gccagcaaat
     6301 ggcgagtgca attattgccg caggtcagga agaggcgcag aaaaataact ggtctgtttc
     6361 cattgctgtt gccgatgacg gcggtcatct gctggcgtta agtcgcatgg acgattgcgc
     6421 gccgattgcg gcttacatct cccaggagaa agcgcgtacc gccgcgctgg ggcgtcgtgc
     6481 aactaagggc tatgaagaga tggtgaacaa cggacgtacc gcgttcgtga ctgcgccgtt
     6541 actgacgtcg ctggaaggcg gcgtaccggt tgttgttgat gggcaaatta ttggtgccgt
     6601 gggcgtttct ggtttaaccg gagcacagga tgcgcaggtc gcgaaagtgg cagcagcggt
     6661 gttggcgaaa taagcgaaaa cgaggagaga aacaatgagt caaaccataa cccagggccg
     6721 tttacgcatt gacgccaatt ttaaacgttt tgtggatgga gaagttttgc cgggcgtgga
     6781 actggacgct gctgcattct ggcacaatgt tgatgagatc gttcacgatc tggcaccaga
     6841 aaatcgtcag ttgctggcag aacgcgatcg cattcaggcg gcgcttgatg agtggcatcg
     6901 cagcaatccg gggccggtaa aagataaagc ggcctataaa tctttccttc gtgaactggg
     6961 ctacctggtg ccgcaaccgg agcgcgtgac ggtggaaacc acgggcattg acagcgaaat
     7021 caccagtcag gcggggccac agctggtggt tccggcaatg aacgcccgct acgcgctgaa
     7081 cgcggcgaac gctcgctggg gctcactgta cgatgcgtta tacggcagcg acatcatccc
     7141 gcaggaaggg gcgatggtca gtggctacga tccgcagcgc ggtgagcagg ttatcgcctg
     7201 ggttcgacgt ttcctcgatg aatcactacc gctggaaaac ggcagctatc aggatgtggt
     7261 ggcgtttaag gtggtcgata aacaattacg catccagttg aaaaatggta aagaaaccac
     7321 gttacgtact ccagcacagt ttgtcggtta ccgtggcgat gccgctgcgc cgacctgcat
     7381 tttgctgaaa aataacggcc tgcatattga actgcaaatt gatgccaacg ggcggattgg
     7441 caaagacgat ccggcgcaca tcaacgatgt tatcgtcgaa gctgctatca gtaccattct
     7501 cgactgcgaa gattcggtcg cggcggttga tgcggaagat aaaatcctgc tgtaccgcaa
     7561 tctgctgggc ctgatgcagg ggactctgca agagaaaatg gagaaaaacg gtcggcaaat
     7621 cgtacgtaaa ctgaatgacg atcgtcagta caccgccgcc gatggctctg aaatttctct
     7681 gcacggacgc tcgttgctgt ttatccgcaa cgtgggtcat ttgatgacca ttcctgtgat
     7741 ttgggacagc gaaggcaatg aaatcccgga aggtattctt gatggcgtca tgactggcgc
     7801 gattgccctc tatgatttaa aagtgcagaa aaactcgcgc actggcagcg tctatattgt
     7861 gaaaccgaaa atgcacggtc cgcaggaagt ggcgttcgcc aacaaactgt ttacccgcat
     7921 tgagacaatg ctcggtatgg caccgaatac cctgaaaatg ggcattatgg atgaagaacg
     7981 ccggacctcg ctgaacttgc gtagctgtat cgctcaggcg cgcaaccgcg tggcgttcat
     8041 caataccggt ttcctcgacc gtaccggcga tgaaatgcat tcggtgatgg aagctggccc
     8101 gatgctgcgt aaaaaccaga tgaaatcgac gccgtggatc aaagcctacg aacgtaataa
     8161 cgtgctttcc ggtctgttct gtgggctgcg cggtaaagcg caaatcggta aaggcatgtg
     8221 ggcaatgccg gacctgatgg cagacatgta cagccagaag ggcgaccaac tgcgtgccgg
     8281 ggcaaacaca gcctgggttc cgtcaccaac cgctgctacg ctccatgcgc tgcactacca
     8341 ccataccaac gtacagagcg tgcaagccaa cattgcccag accgagttca gtgctgaatt
     8401 tgaaccgctg ctggacgatc tgctgactat tccggtggct gaaaacgcta actggtcggc
     8461 gcaagagatc caacaagagc tggataacaa cgtgcagggg attctggggt acgtggtgcg
     8521 ttgggtggag caggggattg gttgttcaaa agtgccggat attcacaacg tggcgctgat
     8581 ggaagaccgc gcaacgctgc gtatctccag ccagcatatc gccaactggt tacgtcacgg
     8641 tattctgacc aaagaacagg tgcaggcgtc gctggagaat atggcgaaag tggttgatca
     8701 gcaaaatgct ggcgatccgg cttatcgtcc gatggcgggg aatttcgcta actcgtgtgc
     8761 ttttaaagct gccagcgatt taatcttcct cggcgtgaaa cagccaaacg gttataccga
     8821 accgttatta cacgcctggc gtttacgcga aaaagaaagt cattaatagc gtcaaaaaag
     8881 ccccggcaag ttttattgtc ggggctatga tagtaactgc ggtagtacat ttatcccgga
     8941 aataaccgga gcgattatca gggtttaaca atattaaata cccgtagcgc attactagcg
     9001 ggcctaaaac aacaatatgg acatcttaac gatactctgc gcctgcgtgt gcggaacaga
     9061 aacccttacc cgatatactt gttatgccag aggtagcaat tgcgttagcg gcgtactgtt
     9121 gctgtaaagt ataacgccta tattttacag ttcagtcctc ttttgcttat tacaacctta
     9181 agtatatcaa gcatataaag ataataagag actgaacaat atggttacct ggacccaaat
     9241 gtatatgccg atgggaggac tggggctatc cgctctggtc gccctgatcc cgataatatt
     9301 cttcttcgtt gcactcgcgg tattacgtct gaaaggacat gttgctggag caataaccct
     9361 tatattatct atcctgattg cgatattcgc ctttaaaatg ccgattgata tggcatttgc
     9421 tgctgcgggc tatggcttta tttatggatt atggccaatt gcgtggatta ttgtcgcggc
     9481 ggtgttcctg tataaattaa ccgttgccag cgggcagttc gatattatcc gcagctcggt
     9541 tatctccatc accgacgatc agcgtttgca ggtgttactg attggtttct cctttggtgc
     9601 gttgctggaa ggagctgctg gctttggtgc gacggtggcg tttggtgcgt tgggcgtgcc
     9661 gattctggtc gccggtcagg taacgggaat cgatccgttc cacattggcg caatggcggg
     9721 acgtcagtta ccgttcctgt cggttcttgt gccgttctgg ctggtagcaa tgatggacgg
     9781 ctggaaaggg gtgaaagaga cgtggccagc ggcgctggtt gctgggggaa gcttcgctgt
     9841 cactcagttc tttacctcta actatattgg tccggaactg ccggatatta cttcggcgct
     9901 ggtgagtatc gtctcactcg ctttattcct taaagtctgg cggccgaaaa ataccgaaac
     9961 ggcaatcagc atggggcaat ccgcaggtgc gatggtggta aataagccat cttctggcgg
    10021 tcccgtgcct tcagaatata gtctggggca aatcattcgt gcgtggtcac cgtttttaat
    10081 cttaacggtg ctggtcacca tctggaccat gaagccgttt aaagcgttat ttgctccggg
    10141 cggcgcgttt tattcactgg tgattaattt ccagatccct catttgcatc aacaagtgtt
    10201 gaaagcggca cccattgtcg cccaaccaac gccaatggat gcggtgttta aattcgaccc
    10261 tctctcggct ggcggcaccg ctatttttat tgcggcgatt atctctatct tcatcctcgg
    10321 cgtgggcatc aagaaaggga ttggcgtctt tgccgaaatg ctaattagtc tgaagtggcc
    10381 gatattgtcg attggcatgg tgctggcatt cgccttcgtc accaactatt ccggcatgtc
    10441 caccacgctg gcgctggtac tggcaggtac aggcgtgatg ttcccgtttt tctcaccgtt
    10501 tctcggctgg ctgggcgtat tccttaccgg gtcggacact tcttcaaacg ccctgtttgg
    10561 ttcactgcaa tcgaccacgg cgcagcaaat caacgtctct gacactctgc tggtggcagc
    10621 aaataccagc ggcggcgtaa ctggcaagat gatctccccg caatctatcg ccgtggcctg
    10681 cgccgcgacg ggcatggtgg gccgagaatc tgaactgttc cgctacaccg tgaagcacag
    10741 tctgattttt gccagcgtta tcaccctgct gcaggcgtat gtgtttaccg ggatgttagt
    10801 ctcgtaaatc aataaagccg gatgaaggta aatatcaccc ggctttccat ttgtctgtcg
    10861 tttatctcgc cagggaaccg tttttacgat tggcttcttt atacgtagtc tccgttttac
    10921 gatgcgaatt gtttaatatt tatacaactt ggcttctgtg ctggttataa agtacccact
    10981 ttcatgagat caattttagt atgttattac attgcttcat ctgttgcttt catgacgaat
    11041 ggcgtttttt ctcttattta tatttttcac tagaacaatg agtagatcaa aattcccaaa
    11101 actcccaaaa acataagatt attcgcaaag ctgattttgc agaataatga gaacttttaa
    11161 gatttgtcga ggatcaattt taagcaattt atctccactt gttacataaa agatgccgtt
    11221 aggttaataa gttaatcttg ctataacagt gttcgttact gcctattaat aaagatattt
    11281 gggatagggt aacgtggatg aggattcgaa atcatccgtc ttgcggaaag tgaaatgttg
    11341 gcagatgtta atattatgta tgtattttaa ctccaacaac acaccaatca ttttgttaga
    11401 aataagaaaa tttatggtta aataatttgg tgattatgaa gaattaaatc gtaatgccaa
    11461 atattgttcg ctttggagag tattattgcc tgtttgtgtc tgttgagcag aa
//
LOCUS       DAZSNG010000120        11097 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZSNG010000120 DAZSNG010000000
VERSION     DAZSNG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.121"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            460..1860
                     /locus_tag="ACHW4Z_003400"
     CDS             460..1860
                     /locus_tag="ACHW4Z_003400"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8882898.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            2016..3551
                     /gene="gadC"
                     /locus_tag="ACHW4Z_003401"
     CDS             2016..3551
                     /gene="gadC"
                     /locus_tag="ACHW4Z_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR8882899.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3682..5001
                     /locus_tag="ACHW4Z_003402"
     CDS             3682..5001
                     /locus_tag="ACHW4Z_003402"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR8882900.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5378..6448
                     /gene="dosC"
                     /locus_tag="ACHW4Z_003403"
                     /pseudo
     CDS             5378..6448
                     /gene="dosC"
                     /locus_tag="ACHW4Z_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            6448..7065
                     /locus_tag="ACHW4Z_003404"
                     /pseudo
     CDS             6448..7065
                     /locus_tag="ACHW4Z_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            7323..7655
                     /gene="ddpX"
                     /locus_tag="ACHW4Z_003405"
                     /pseudo
     CDS             7323..7655
                     /gene="ddpX"
                     /locus_tag="ACHW4Z_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7669..8565
                     /gene="ddpC"
                     /locus_tag="ACHW4Z_003406"
     CDS             7669..8565
                     /gene="ddpC"
                     /locus_tag="ACHW4Z_003406"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR8882901.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8562..9536
                     /locus_tag="ACHW4Z_003407"
     CDS             8562..9536
                     /locus_tag="ACHW4Z_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8882902.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9529..10455
                     /locus_tag="ACHW4Z_003408"
                     /pseudo
     CDS             9529..10455
                     /locus_tag="ACHW4Z_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(10511..10942)
                     /gene="osmC"
                     /locus_tag="ACHW4Z_003409"
     CDS             complement(10511..10942)
                     /gene="osmC"
                     /locus_tag="ACHW4Z_003409"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR8882903.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2747 a         2712 c         2757 g         2881 t
ORIGIN      
        1 ttgttgaaac aaatgacggt agagaatgtt aacactgccg ttaaacaata tctttctcat
       61 ccggtaaata cttataccgg agtattattg ccaaaataat aacagccccg tcaacacatc
      121 gttggcgggg attttagcaa tattcgctat ttttatgtaa taattttata aatgcgttca
      181 aaataataac caagtactaa tagtgatatt ttaatacctg atttttacgt gataattcag
      241 gaggcacaga aagcgcataa aaataacaac ataaaacacc ttgccaccac ccaagaattc
      301 tctattacat tgtttttaat gaaaaaatat cattccaaca atatctcacg ataaataaca
      361 ttaggatttt gttatttaaa cacgagtcct ttgcacttgc ttactttatc gataaatcct
      421 acttttttaa tgcgatccaa tcattttaag gagtttaaaa tggataagaa gcaagtaacg
      481 gatttaaggt cggaactact cgattcacgt tttggtgcga agtctatttc cactatcgca
      541 gaatcaaaac gttttccgct gcacgaaatg cgcgacgatg tcgcatttca gattatcaat
      601 gatgaattat atcttgatgg caacgctcgt cagaacctgg ccactttctg ccagacctgg
      661 gacgacgaaa acgtccacaa gttgatggat ttatccatta acaaaaactg gatcgacaaa
      721 gaagaatatc cgcaatccgc agccatcgac ctgcgttgcg taaacatggt tgccgatctg
      781 tggcatgcgc ctgcgccgaa aaatggtcag gccgttggca ccaacaccat tggttcttcc
      841 gaggcctgta tgctcggcgg gatggcgatg aaatggcgtt ggcgcaagcg tatggaagct
      901 gcaggcaaac caacggataa accaaacctg gtgtgcggtc cggtgcaaat ctgctggcat
      961 aaattcgccc gctactggga tgtggagctg cgtgagatcc ctatgcgccc cggtcagttg
     1021 tttatggacc cgaaacgcat gattgaagcc tgcgacgaaa ataccatcgg cgtggtgccg
     1081 actttcggcg tgacctacac cggtaactat gagttcccgc aaccgctgca cgatgcactg
     1141 gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc cgccagcggt
     1201 ggctttctgg caccgttcgt cgccccggat atcgtctggg acttccgcct gccgcgtgtg
     1261 aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg cggctgggtt
     1321 atctggcgtg acgaagaagc gctgccgcag gaactggtgt tcaacgttga ctacctcggt
     1381 ggtcagattg gtacttttgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
     1441 tactatgaat tcctgcgcct tggtcgtgaa ggctatacca aagtacagaa tgcttcctac
     1501 caggtcgctg cctatctggc ggatgagatc gccaaactgg ggccgtatga gttcatctgt
     1561 accggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaagat
     1621 ccgggataca ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg
     1681 gccttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat gtgtcgtcgc
     1741 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
     1801 ctcagcgatc acccgaaact gcagggtatt gcccagcaga acagctttaa acatacctga
     1861 taacgtttaa cggtaacggt gtcccgaaac gaacccgttt cgggataatt tccaaagtct
     1921 gttcactggc attagcaacg gaaaatattg ttctgaatac gcttcagaac aaaacaggtg
     1981 cggttccgac aggaataccg ttttaggggg ataatatggc tacatcagta cagacaggta
     2041 aagctaagca gctcacatta ctcggattct ttgccataac ggcatcgatg gtaatggctg
     2101 tttatgaata ccctaccttc gcaacatcgg gcttttcatt agtcttcttc ctgctattag
     2161 gcgggatttt atggtttatt cccgtgggac tttgtgctgc ggaaatggcc accgtcgacg
     2221 gctgggaaga aggtggtgtc ttcgcctggg tatcaaatac tctgggtccg agatggggat
     2281 ttgcagcgat ctcatttggc tatctgcaaa tcgccattgg ttttattccg atgctctatt
     2341 tcgtgttagg ggcactctcc tacatcctga aatggccagc gctgaatgaa gaccccatta
     2401 ccaaaactat tgcagcactc atcattcttt gggcgctggc attaacgcag tttggtggca
     2461 cgaaatacac ggcgcgaatt gctaaagttg gcttcttcgc cggtatcctg ttacctgcat
     2521 ttattttgat cgcattagcg gctatttacc tgcactccgg tgcccccgtt gctatcgaaa
     2581 tggattcgaa gaccttcttc cctgacttct ctaaagtggg cacactggtt gtatttgttg
     2641 ccttcatttt gagttatatg ggcgtagaag cttccgcaac tcacgtcaat gaaatgagca
     2701 accccgggcg cgactatcca ctggctatgt tactgctgat ggtggcggca atctgcttaa
     2761 gctctgttgg cggtttgtct attgcgatgg tcattccggg taatgaaatc aacctctccg
     2821 caggggtaat gcaaaccttt accgttctga tgtcccatgt ggcaccggaa attgagtgga
     2881 cggttcgcgt gatctccgca ctgctgttgc tgggtgttct ggcggaaatc gcctcctgga
     2941 ttgttggtcc ttctcgcggg atgtatgtca cagcgcagaa aaacctgctg ccagcggcat
     3001 tcgctaaaat gaacaaaaat ggcgtaccgg taacgctggt catttcgcag ctggtgatta
     3061 cttctatcgc gttgatcatc ctcaccaata ccggtggcgg taacaacatg tccttcctga
     3121 tcgcactggc gctgacggtg gtgatttatc tgtgtgctta tttcatgctg tttattggct
     3181 acattgtgtt ggttcttaaa catcctgact taaaacgcac atttaatatc cctggtggta
     3241 aaggggtgaa actggtcgtg gcaattgtcg gtctgctgac ttcaattatg gcgtttattg
     3301 tttccttcct gccgccggat aacatccagg gtgattctac cgatatgtat gttgaattac
     3361 tggttgttag tttcctggtg gtacttgccc tgccctttat tctctatgct gttcatgatc
     3421 gtaaaggcaa agcgaatacc ggcgtcactc tggagccaat caacagtcag aacgcaccaa
     3481 aaggtcactt cttcctgcac ccgcgtgcac gttcaccaca ctatattgtg atgaatgaca
     3541 agaaacacta accatgtctg aatggttata agacaaggga gcgataattc atcgctccct
     3601 ttttcgtgct tgctgtcgta ttgacttaac cgggataagt acgagaatga gcgcacatct
     3661 gtttaccgga aaccagcaca tatggatatc tgctcccgaa acaagaaatt aacgattaga
     3721 agaccagcga tactggttgc attggcactt ttactgtgta gttgtaaaag cacgcctcca
     3781 gagtccatgg tgacaccacc agcaggttca aagccaccag ccacgacgca acaatcgtca
     3841 caaccgatgc gtggcatctg gctggccacg gtttctcgcc tcgactggcc accggtttcc
     3901 tcggttaaca ttagtaatcc caccagccga gcccgtgtac aacaacaggc gatgatcgac
     3961 aaactggatc atctccaacg tctcggcata aacacggtct ttttccaggt caagccggac
     4021 ggtaccgccc tgtggccatc gaaaattttg ccgtggtccg atcttatgac cggtaagatt
     4081 ggtgaaaatc cgggttacga tccgctgcaa ttcatgctcg acgaagccca caagcgtggg
     4141 atgaaagtac acgcctggtt taacccctat cgcgtatcgg ttaatacgaa gcccggtact
     4201 atcagggaac tgaatagcac tctgtctcaa caaccggcga gcgtctatgt gcaacaccgt
     4261 gactggatca gaacgtctgg cgatcgcttt gtcctcgacc cgggcatccc tgaggttcag
     4321 gactggatca catcaatagt cgctgaagtg gtttcccgct atccggtaga tggcgtgcag
     4381 tttgacgact atttctatac tgaatcaccg ggttcacggc taaatgataa cgaaacgtac
     4441 cgtaaatacg gaggcgcatt tgcgtcaaaa gcagactggc ggcgcaacaa tactcagcag
     4501 ttaattgcaa aggtatcaca caccattaaa agcattaagc cgggagtcga atttggcgtt
     4561 agcccggcag gcgtgtggcg taaccgatca cacgatccgc tcggttccga tacccgaggc
     4621 gcggcagcct atgacgaatc ctacgcagac acccgtcgat gggtggaaca aggattgctg
     4681 gattacattg ctccccaaat ttactggccg ttctcacgga gtgccgcgcg ttatgacgtg
     4741 ttggcaaaat ggtgggcgga tgtcgttaaa ccaaccagga cccgcctgta tatcggtatc
     4801 gccttctata aagtgggtga accttcaaag atagagccag actggatgat taacggcggc
     4861 gtaccggaac tgaaaaagca gctcgatctt aacgatgcgg taccagaaat tagcggcacc
     4921 atcttgttcc gtgaggacta tctgaataaa ccgcagactc aacaagcggt cagctatctg
     4981 caaagtcgtt ggggcagtta aactaccatg cttactttgt aacaagccgg tgctttaccc
     5041 accggcttgt tacaccttgt gaaatatccc actagtaatc atgcttacat aagtcaaatt
     5101 aaccacgcaa ttaaccacac ttagttaaac agctataaat ccctaataaa aatgtatgaa
     5161 tatgaaaaac catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt
     5221 ctgaagaaca gtagcgtaga ttgaaactgt cttaagtcgc ttatttcttt tcagtatatc
     5281 ttcatatttc aggagaatat ggtgcagtta gtcgatctcg cacgttgcgt ttcgttttga
     5341 ttccgtctga tttcccagcc ttataagggt gtgaattatg gagatgtatt ttaaaagaat
     5401 gaaagatgag tggaccggac ttgtcgaaca ggcagatcct ctcattcgtg ctaaagccgc
     5461 agaaattgcc cttgcgcatg ctcattatct gagtatcgag ttttatcgaa ttgtccgcat
     5521 cgacccgcat gccgaagaat tcttgagtaa tgaacaagtt gagcggcagt tgaaaagtgc
     5581 gatggaacgc tggattatta acgtgctttc tgcccaggtt gacgatgtcg aaaggctaat
     5641 acaaatccag cataccgtcg cggaagtgca tgcccgcata ggaattccgg tagaaattgt
     5701 cgagatgggg tttcgggtgc tgaaaaaaat cctctatccg gtaatcttct cttcggatta
     5761 ttccgccgca gaaaaacttc aggtctacca tttctcgatt aacagtattg atatcgcgat
     5821 ggaagtgatg acccgcgcgt ttacctttag tgacagtagt gcctcaaagg aagatgaaaa
     5881 ctatcgtatc ttctcgttac tggaaaacgc cgaagaagaa aaagaacggc aaatagcctc
     5941 aatactttca tgggaaatag atattatcta taaagtcctg ctggattctg atttaggcag
     6001 cagtttgcct ttaagccagg ctgattttgg cctgtggttt aaccataaag gtcgacatta
     6061 ttttagcggc attgccgaag tgggccatat ctcccgtctg attcaggatt tcgacggtat
     6121 tttcaatcaa accatgcgta acaccaggat tttgaataac agaagtttgc gggtgaaatt
     6181 tctattacag ataagaaata ccgtatcgca gattattacc ttgctgcgtg aattgtttga
     6241 agaagtatcg cgccacgaag tcggtatgga tgtactgacg aaattactta accgccgttt
     6301 cctgccgact atcttcaaac gcgaaattgc ccatgccaac cggaccggta caccgctgtc
     6361 agtgctgatt attgacgttg ataaattcaa agagatcaac gatacgtggg gccataacac
     6421 tggcgatgaa attctgcgta aagtctcatt cctctcgcag aagagattgg tgaaatcgaa
     6481 aatattgggc gctgggtcat cgcggaagct tgccgtcagt tagcagaatg gcgtagccag
     6541 aatattcata tcccggcgtt atccgtgaac ttgtcggcgc tgcactttcg cagtaatcag
     6601 ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta ttgacggcca ccagctgacg
     6661 gtggaaatca cggaaagcat gatgatggaa cacgataccg aaatctttaa gcgcattcag
     6721 atcctgcgtg atatgggcgt gggtttatcg gtagatgatt ttggcacggg cttttccgga
     6781 ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa ttgacaaaag ctttgtcgat
     6841 cgttgtctga cggaaaaacg catcctggcc ttacttgaag ccattaccag cattgggcaa
     6901 agcctcaatt taaccgtcgt ggcggaaggg gtcgaaacca aagagcaatt tgagatgcta
     6961 cgtaagatcc actgtcgcgt tattcaggga tatttctttt cccgccccct tcccgccgaa
     7021 gaaattccag gctggatgag cagcgtgtta ccgctgaaaa tctgacaaat tcctctcgcc
     7081 cgcactcgcg ggttttcctt taaggtgaca ctgtcactta aatactgtga tttcagctac
     7141 gattccggga gattccttcc ttaactttcc ctcttgcacc aaaactgcac tacgctgcac
     7201 aaatgttgat caaaagttaa aaaatataac tttaagccag cattctggcg cttatcccgg
     7261 catggcatga gatctgcata agcggaaagc gcagcaattt ttgtcttata caggaaccgt
     7321 ttatgtcgga taccaccgaa ctggttgatt tagccgtgat cttccctgat ctggagatcg
     7381 aattgaaata tgcctgcgtt gataacatca caggtaaagc tatttatcag caagcgcgtt
     7441 gtctgctaca taaagatgcg attaccgcgc tggcgaaaag tatcagtatc gcccagctgt
     7501 caggcttaca actggtgatt tacgatgcgt atcgcccaca acaagcacag gcgatgttgt
     7561 ggcaagcctg cccagacccg caatatgttg ttgatgtgac ggtcggttct aatcacagcc
     7621 gtggcacggc gctcgacctg acgcttcgtg atgacgtgga ggtggtgaat gatgctaagc
     7681 gaggaaacgt ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc aaaactggta
     7741 tggatgctga aaggcagtcc gctcaccgtg accggcgcag tcatcattgt attaatgcga
     7801 ctgatgatga ttttttcacc gtggctggcg acgcatgatc ccaacgccat tgatttaacc
     7861 gcccgccttt tgccgccttc tgcggcgcac tggtttggca ccgatgaagt gggacgcgat
     7921 ctgtttagcc gcgtactggt cggcagtcag caatcaattc tcgccggatt agtggtggtc
     7981 gccattgcgg gtatgattgg ttcgctactc ggatgtctat ccggtgtgct tggcggacgc
     8041 gcagacgcca tcatcatgcg catcatggac attatgctgt cgattccttc gttggtactg
     8101 acaatggcac tggctgccgc tctcgggccg agtttattta acgccatgct ggcgattgct
     8161 attgtgcgaa ttccctttta tgtgcgcttg gcgcgggggc aagcattagt tgtacgccag
     8221 tatacctatg ttcaggcggc gaaaaccttt ggcgcgtctc gttggcatct gatcaactgg
     8281 catattttac gtaactccct accgccgctg atcgtgcagg catcgctgga tatcggtagc
     8341 gcgattttaa tggccgccac gttgggattt attggcctgg gtgctcaaca accgagtgct
     8401 gaatggggcg cgatggtagc gaatggtcgc aactatgtac tcgatcagtg gtggtattgc
     8461 gcatttccgg gggcagcgat tttgcttacc tccgtcgggt ttaatctctt tggtgatggt
     8521 attcgcgatc tgcttgaccc gaaagcagga ggaaagcagt catgacccaa cccgttctgg
     8581 acattcaaca gctgcatttg agtttccccg gttttaacgg tgatgttcac gcgctcaaca
     8641 atgtgtcctt gcagattaac cgcggcgaaa ttgtcggtct ggtgggagaa tccggctcag
     8701 gtaaatcagt caccgcaatg ctgattatgc gtctgctacc gacgggcagt tattgcgtac
     8761 atcgggggca gatttcactg ctgggagaag atgttcttaa cgctcgggaa aaacagcttc
     8821 gtcagtggcg cggcgcacga gtggcgatga tctttcagga accgatgacc gcccttaatc
     8881 cgacacgtcg aataggtctt cagatgatgg acgtaatccg ccatcatcaa ccaataagtc
     8941 gtcgggaagc cagagctaaa gcgattgcac tgctggaaga gatgcaaatc ccggatgccg
     9001 tggaagttat gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag cgggtaatga
     9061 ttgcgccggc attctcctgc gagccgcaat tgattattgc cggcgaaccg acgacggcgc
     9121 tggacgtcac agtacagttg caggtactgc gtttgcttaa acataaagca cgcgccagtg
     9181 gaactgcggt actgttcatc agccatgata tggccgtggt gtcgcaactg tgcgatagcg
     9241 tttacgtgat gtatgccgga agcgtaattg aaagcggcgg gacggcagac gttatccatc
     9301 atccccggca tccgtatacc attggtttgc tgcaatgcgc accagaacat ggagtaccac
     9361 gccagccatt acccgccatt ccagggacgg taccaaacct cacccatttg cctgacggct
     9421 gcgcttttcg cgatcgttgc tatgcggcag gtgcacagtg tgcgctgaca gcgtgtggtg
     9481 acaacaacca gcactgcacc tgttggtatc cgcagcagga ggtcataagt gtctgacacg
     9541 ttattaacgt tacgcgacgt ccatatcaat tttccggccc gtaaaaactg gcttggcaaa
     9601 tctacggaac atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc
     9661 ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggc
     9721 atgctgcaac cgagccacgg gcagtacatc cgttcaggct cacaacgcat tatgcagatg
     9781 gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg catcatcaca
     9841 gaatcgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt ggcagaggag
     9901 ttggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc
     9961 ggcgggcagc ggcaacgcat cgccattgcc agagcactct cttcgcagcc tgacgtgatt
    10021 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaatttg
    10081 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    10141 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    10201 ggcgacgcgc aacaggtgtt gactgcttcg gcacatccat acacccgatt attgctggat
    10261 tccctccccg ccattgataa accgctggag gaagaatgag cattacgtaa aacggatctg
    10321 ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc tctggcaacc
    10381 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga cctccggtgc
    10441 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    10501 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    10561 agaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    10621 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt
    10681 tatccagcga cacatcggcg gtggtatcaa ttgatgttgg cgtgaatccc gcttccccca
    10741 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    10801 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    10861 cgccgctctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    10921 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    10981 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt
    11041 acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaaga
//
LOCUS       DAZSNG010000121        11036 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZSNG010000121 DAZSNG010000000
VERSION     DAZSNG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.122"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            157..504
                     /locus_tag="ACHW4Z_003410"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHW4Z_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHW4Z_003411"
     CDS             557..952
                     /locus_tag="ACHW4Z_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR8882904.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHW4Z_003412"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHW4Z_003412"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR8882905.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHW4Z_003413"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHW4Z_003413"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR8882906.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHW4Z_003414"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHW4Z_003414"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR8882907.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHW4Z_003415"
     CDS             complement(5323..6423)
                     /locus_tag="ACHW4Z_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR8882908.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHW4Z_003416"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHW4Z_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR8882909.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="ACHW4Z_003417"
     CDS             complement(7571..8137)
                     /locus_tag="ACHW4Z_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8882910.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="ACHW4Z_003418"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="ACHW4Z_003418"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR8882911.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10128..10172)
                     /locus_tag="ACHW4Z_003419"
     CDS             complement(10128..10172)
                     /locus_tag="ACHW4Z_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882912.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            10263..10751
                     /locus_tag="ACHW4Z_003420"
     CDS             10263..10751
                     /locus_tag="ACHW4Z_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR8882913.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="ACHW4Z_003421"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="ACHW4Z_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2690 a         2815 c         2634 g         2897 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagt
//
LOCUS       DAZSNG010000122        10979 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZSNG010000122 DAZSNG010000000
VERSION     DAZSNG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.123"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHW4Z_003422"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHW4Z_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR8882914.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHW4Z_003423"
     tRNA            complement(621..695)
                     /locus_tag="ACHW4Z_003423"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHW4Z_003424"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHW4Z_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHW4Z_003425"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHW4Z_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR8882915.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /gene="yfdF"
                     /locus_tag="ACHW4Z_003426"
     CDS             complement(2932..3990)
                     /gene="yfdF"
                     /locus_tag="ACHW4Z_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR8882916.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="ACHW4Z_003427"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="ACHW4Z_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR8882917.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="ACHW4Z_003428"
     CDS             6068..6352
                     /locus_tag="ACHW4Z_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR8882918.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="ACHW4Z_003429"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="ACHW4Z_003429"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR8882919.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="ACHW4Z_003430"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="ACHW4Z_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR8882920.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="ACHW4Z_003431"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="ACHW4Z_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR8882921.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2742 a         2528 c         2730 g         2979 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatat
//
LOCUS       DAZSNG010000123        10870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZSNG010000123 DAZSNG010000000
VERSION     DAZSNG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.124"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            233..1063
                     /locus_tag="ACHW4Z_003432"
     CDS             233..1063
                     /locus_tag="ACHW4Z_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8882922.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1204..1977)
                     /gene="uxuR"
                     /locus_tag="ACHW4Z_003433"
     CDS             complement(1204..1977)
                     /gene="uxuR"
                     /locus_tag="ACHW4Z_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR8882923.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2192..3652)
                     /locus_tag="ACHW4Z_003434"
     CDS             complement(2192..3652)
                     /locus_tag="ACHW4Z_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR8882924.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3733..4917)
                     /gene="uxuA"
                     /locus_tag="ACHW4Z_003435"
     CDS             complement(3733..4917)
                     /gene="uxuA"
                     /locus_tag="ACHW4Z_003435"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR8882925.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5257..6600
                     /gene="gntP"
                     /locus_tag="ACHW4Z_003436"
     CDS             5257..6600
                     /gene="gntP"
                     /locus_tag="ACHW4Z_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR8882926.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6851..7753)
                     /gene="fimH"
                     /locus_tag="ACHW4Z_003437"
     CDS             complement(6851..7753)
                     /gene="fimH"
                     /locus_tag="ACHW4Z_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR8882927.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7773..8216)
                     /locus_tag="ACHW4Z_003438"
     CDS             complement(7773..8216)
                     /locus_tag="ACHW4Z_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8882928.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8289..8819)
                     /gene="fimF"
                     /locus_tag="ACHW4Z_003439"
     CDS             complement(8289..8819)
                     /gene="fimF"
                     /locus_tag="ACHW4Z_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR8882929.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8829..>10870)
                     /gene="fimD"
                     /locus_tag="ACHW4Z_003440"
     CDS             complement(8829..>10870)
                     /gene="fimD"
                     /locus_tag="ACHW4Z_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR8882930.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2617 a         2799 c         2661 g         2793 t
ORIGIN      
        1 agcaatatca aattccaatt agtaataaga tatgctttta aattgcatgc aaataatttt
       61 aaatgagcgt tttttgatac tttagagtgt ttcctaccct ttaatatcac tgtgtagagt
      121 tgatatcact acaacagaaa catgcatgtc atttgtttac ttctcttttt actacagaaa
      181 aatttatcta tttttctaaa catcagttaa atactcataa aaggtaacta ttatgtctat
      241 gccattaagc aatgccgcta ataaccaatt caatacaaat aatcacttcc ttcatcatcc
      301 aaaagtcgac agtgagttaa cacgtcgata tgaatatgca cgcctggata cggaaaatat
      361 ctatcttctg cctcttgctc gtggcaataa ccataattac gatggaaaaa gcgttgtcga
      421 aattagaaaa cttaatattg gcaaacagtc ctggccattt aattatgtga cagatacatg
      481 cagagaattc aatggtatta caacaacagg acgtatgctc tatcgaaact tgaaaatcac
      541 ctcagcttta gacgaaatat atggtggtat atgtaaaaag gcgcatgctg ctacagagtt
      601 agcagaaggt ttacggctaa atttatttat gaagagccct ttcgatcctg ttgaggacta
      661 tacagttcat gaaataacat taggccctgg ttgcaatgtt cctggttatg ccggaaccac
      721 gataggatat atttcaaccc ttccagcttc tcaggcaaaa agatggacca atgagcagcc
      781 aagaattgat atctatattg atcagataat aactgtctca ggggtagcaa attcttccgg
      841 attcgccctt gccgcattac ttaatgctaa tattgagatg ggaaatgacc caatcattgg
      901 tatagaagcc tatcctggta ccgcggagat ccattcaaaa atgggatata acgttatccc
      961 cggtgatgaa gatgcgccac tcaaacgcat gactctgcaa ccgtcatctc ttccagaact
     1021 gtttgaatta aaaaatggtg aatggaatta tattggtaaa tagccactga gaagaatgag
     1081 tactaatatt caggttatac agatatttaa acaaaaccct gcaatttaca aagtaatact
     1141 cattgcgact tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat
     1201 aagtcaggcg tcgactttat ccagcggcca tgaatcaaag agatagccgt caaaataaat
     1261 atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat
     1321 tgccagcttc gccgctcggg catctttttt gattaacgcg gcgaggatct gtttgtgatc
     1381 gcccaaccac tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat
     1441 tggattgttt tcccgccact gccaggactg acggaataac tccaccagca tgctgttatg
     1501 cgttgcttca gcaatggcga gatggaactg catgtcaccg ctttcgctgc tacccggcgc
     1561 gctggaagcc agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc
     1621 gcgggtagcc tgcagtgcgg caaactcggc gatgttactt tccagtaact gacgcgcctg
     1681 taacagctca aaaggacccg catcattgca gacgttggca tccggactgt ctgtgttgtg
     1741 gctgcctgag ctatcaagca catagatacc ggcaccccgg cgcacttcca ccagcccttt
     1801 gatctccagc atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc
     1861 aatttcacgc tccggcggca gccgttcgcc aggattgtac ggcgtcttta tgatcagatc
     1921 gtggatcatc gccccgactt cctggtaagg tctttgcgca gaggtgacag atttcatggt
     1981 gttgtgtccg ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc
     2041 cggcctacaa cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg
     2101 aagttggcac gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat
     2161 tgaccagtaa gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac
     2221 cgcgttcgca cagctgctga tatgccgacg tcaccgcgcc aacaaagtcg gcattctgcg
     2281 gcagatcatc ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct
     2341 gatactgcgc gttaatcttc tggaactcgg ccagcatcgg gtcgaccacg tcaatggcat
     2401 taccctgctc atccacgccc tgggtgtaac ccatccagcc agccacgccc agcgccaggt
     2461 gacgccagct gccgccgttt tgcaggtgca gacgcaccgg gtccagtaga cgctgcggta
     2521 acttctggct gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga
     2581 aacgctcgat cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg
     2641 ttggcgcttg ttcctgcatc atcagggcaa aggccgcttt gcgataatcc gggttagtca
     2701 tggtgtcggc aatggtttca tagccgccga ggtaaccgag gtacgccagg aaagagtggc
     2761 tgccgttcag catacgcagc ttcatcattt cgaacggcac aacgtctgca acgaactgtg
     2821 tgcccacttt atcccagtcc gggcgaccat taacgaagtt atcttcaatc acccactgac
     2881 ggaacggttc gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct
     2941 gtaaggtttc tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga
     3001 cgttctcttc aatccatgcc gctagctgcg ggtcacgcgc ctgagccagc cccagtaccg
     3061 cgaccttcgc cacatggccg ttttcacgca cgttatcgca ggacatcacc gtaaatgctt
     3121 tcagcccttt ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc gcggacttcg
     3181 gcgcagtcgg gttttccaga tcgtgcttga tcagcgggtt attgagatcc agttgaccgc
     3241 ttgccgcatc agcgcagtag cctttttccg tgaccgttag agagacaatc gccgtttgcg
     3301 gacgcgccat cgcgttgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt
     3361 tcatcgaacc gataattttc agctcggtgc tttctgcgcc tttttccgct acggtgtaca
     3421 gcagttgctg ttttttcagg ttttcgatca gcatgcggtc gttgcctggc atcaggttaa
     3481 cttcgcagat gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct
     3541 ggtgcgcgcg gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat
     3601 gatcccatga cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact
     3661 ccagaatccg tcttcagaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca
     3721 tgccggtggg tattaacgga taaagaaagc gcgctggatc gccagttcga caccgcgaac
     3781 ttcggccagg cctttcagac gaccaattgc ggagtaacca gggttggttt tcttcttcag
     3841 gtcgtccagc atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc
     3901 cgctttacga cggtgttctt cttcaacaat cgctttcacc actttgtaca tatcaacgtc
     3961 accgttcagg tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg
     4021 cagatgggtg aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc
     4081 agcacgcacg ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt
     4141 atcaaccatc cactgcatat cttcaatggt ggaaacaatg cgcggcaagc cgaggatcgg
     4201 gcgcggcgga tcgtccgggt gaaccgccat acgcacgcca acttcttcag caactggaat
     4261 aatcgctttc aggaagacgg caaagttttc gcgcagtttg gctttgtcga tatctttgta
     4321 cagctccagg tgcttacgga actggtcgag ggtataccct tcttccgcgc ccggcagacc
     4381 ggcaatgatg ttacgcgtca gacgcgcttt gtcttcgtcg ctcatagtgg cgaagcgttc
     4441 agctgcctga gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat
     4501 atgcatttcg aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg
     4561 cagcacgtat tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac
     4621 ggtgcgaatg ccacactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg
     4681 ctcatagttg ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga
     4741 ccacaccagg cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca
     4801 tacttcgccg ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg
     4861 gacatcagct aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat
     4921 ttttcctctt tcacatagtg ggttccacag caaagagacg cggaaccttg gagttgttta
     4981 ggtatccgga tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata
     5041 agttctacct tttggtaaga catgtcaacc agaattctga aattggttaa ccgcgtcgca
     5101 agaatttcac tctcatggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg
     5161 atcagaggat gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca
     5221 aaataacaac ggttcaacca agtaaggtaa aacgttatgc atgtgcttaa cattctctgg
     5281 gtggtattcg gcattggcct gatgctgata ctgaatttga agttcaaaat caattcaatg
     5341 gtggctttgt tggtggcggc gctgtccgtc gggatgctgg caggcatgga tttgatgtcg
     5401 ctgctgcaca ccatgaaagc gggcttcggc aacacgctgg gagaactggc tatcatcgtg
     5461 gtgttcggtg cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagca
     5521 catacgctgc tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc
     5581 cttattttcg gtctggcgat gttctatgaa gtggccttta tcatgttagc gccgctggtt
     5641 attgttattg ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca
     5701 gctgccacta ccgcacattc actgttccca ccgcagccgg gtccggtggc gctggtgaat
     5761 gcttatggcg cggatatggg gatggtttat atctatggcg tactggtgac gatcccaagt
     5821 gtaatctgcg caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca
     5881 tcattcctga aagcagatca accggtagat atgaataatc tgccctcttt cggcgtttcg
     5941 attctggtgc cgctgatccc ggcgatcatt atgatctcta ccaccatcgc caatatctgg
     6001 ctggtaaaag atacccctgc ctgggaagtg gttaacttta ttggttcctc gccgattgcg
     6061 atgtttattg cgatggtggt tgcattcgta ctctttggca ccgcgcgtgg tcatgacatg
     6121 cagtgggtga tgaacgcttt tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc
     6181 atcggtgcgg gtggcgtgct gaagcagacc atcatcgaca ccggcattgg cgacaccatc
     6241 ggcatgttga tgtcccacgg caatatctcg ccctacatta tggcatggct gatcactgtg
     6301 ctaattcgtc tggcgacggg tcagggtgtc gtttcggcga tgaccgccgc cgggattatc
     6361 agtgctgcaa tccttgatcc agcaaccggt cagctggttg gcgtgaatcc ggcgctgctg
     6421 gtactggcga cggctgcggg ttccaacacc ctcacccaca ttaatgatgc atctttctgg
     6481 ttgttcaaag gttactttga cctgtcagta aaagacacgt tgaaaacctg gggtctgctg
     6541 gagctggtca actccgtggt tgggctgatt attgtgctga ttattagcat ggtagcgtaa
     6601 aagaacacca gagcctgcca atggcaggct cagactgatg actaaagtct aaaaatgtgc
     6661 ccggacagtc ccctcgcccc tccggggaga gggttagggt gaggggaaca gaccagtaaa
     6721 gtgcgctttg tttatgccag atgcgacgct gacgcgtctt atctggccta caaagggcta
     6781 acgtgcaggt tttgtaggtc tgatagcgta gcgcctcagg taccagcatt agcaatgcgc
     6841 tgtaattttt ttattgataa acaaaagtca cgccaataat cgattgcaca ttccctgcag
     6901 tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc cagacttacc gccgaagttc
     6961 ctactgctcc taacgatacc gtgttattcg ctggaataat cgtaccgttg cgcgtcaact
     7021 gtacgccgac gccctgcgct ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg
     7081 catctgcggt tgtgccggag aggtaatacc ccaggttttg gcttttcgca caataaacgg
     7141 taagaggaat tggcactgaa ccagggtagt ccggcagagt aacggtgaca tcacgagcag
     7201 aaacatcaca accgccagtg ggcaccacca catcattatt ggcgtaaata ttccacacaa
     7261 actgaaaatc atcgctgtta tagttgttgg tctgtcgcaa aataagcacg gcaattaatg
     7321 aaccagcttt aatagccacc ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg
     7381 gccacggctt atccgttctc gaattataaa caacccgcgg cgtttcgctg gtggtcggaa
     7441 atggatagct actgccacta tattttacgg tcccggaaaa attagataac acgccgccgt
     7501 aagccgagcc tcgttgcagt gtgacatagt ctgtaatggt ttccggataa tcgttatggc
     7561 aaaagatttg cgtcgaaaga tctacgacca ggttttgccc cacattcacg acaggcgcaa
     7621 ggtttacata aacattagcg ctgccaccgc caatagggat agcggtacca ttggcggttt
     7681 tacaggcgaa tgaccaggca tttaccgacc agcccatcag cagtacagca aacagggtaa
     7741 tagctcgttt cattacaatt atctcttcgg gttcagctgt aggtataggt gatgctaatc
     7801 actgcctgaa tggttccctg agtggctccg ccatttaccg tcaatgctct gacctgtaac
     7861 gggaagtgcg ctgattgtga ggaatcatcc acctgaactg ttttggttgc gccagtattc
     7921 aatgtgttgc cactgtcatc ctgtagctct aactggatgt tttgcgcggt cccctggttt
     7981 ttataatatc cggtgctgtc ggctgccccg ctgaagctgg cagtgaccct cgacgttccc
     8041 accggacaat tagtcaactc aagcgcaaca tcatgccagg ccgatgccgc cccggcagac
     8101 ataagactga aagaataaag atcgccgaga tctaccgtgg cattggtggt ggaaaccgta
     8161 cacggtttgg cgacgacctt accgttcacc gtgatggtga catcggctgc ctgtatcgtc
     8221 gcacttgcga gcgccaacat tgccgccaat acatacccac gtttgcacca tttcatgagc
     8281 atctccagtt actgatattc aagagtgaag gtagccgtag cattgatatg ccccgcagtg
     8341 acaggcacct gtgtcgccat tagccgggcg taaaaattca gcgtatttgg tttacccggc
     8401 gtcagggtcg tccacgaaat cgcagacgat ggagcattaa ggggtatttg attttgctgc
     8461 tcattcagaa gctgtattcc cagtcccgca gccgctgaca ccgtattttc aagtgcaagc
     8521 aggttggcat tgtggctatc tgcaacgccg gtaaacccaa cttttacggc agaaacggca
     8581 ttaccacagg gtgacagcaa aatacgaaat ggaacgacag gagtcgtcgc gccaatgttg
     8641 ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa aattggttga ttcagcggcc
     8701 acactacagc cgttatctct gacatagccg cggatagtaa tcgtgctatc cgcagccaaa
     8761 acgcgactta ccgccagcca caaaaaagcg cacagaagat aaaaaggttt gtttctcatc
     8821 acgccccctt aacgacattc agctgatagc tgggttaata actgctgctg actctctggt
     8881 ggcagttgat aattggcgac acagtgagca ttttcctctt ctccccattt cacctgaact
     8941 tttcccgcta aaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta
     9001 ctctggctac tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgagtt
     9061 agcgtcatga gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca
     9121 cgagtgggaa caacgttagc gaccgcgtta tctaaatcga cgttatcagc caaggtattg
     9181 gtatccagcg ccactctatt ttcccgatat tcagtggcat aaggcagcac ggcataacca
     9241 cgccagtcgg tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct
     9301 ttaacaagca ccaccgtatc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc
     9361 aataccccac cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg
     9421 atattggcat tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt acttccgcta
     9481 ttaccatcgc ctcccccggc atagccggtt tgcacgctat aactgaggtt gttgtcttcc
     9541 agcaacgtac cgtatacacc agccagattg gtcatccgac cgttgagatc gtgtgacatg
     9601 ctgtagctgg cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg
     9661 aaaggaatat tgacgttaag cgctaacatc tgatcccgtc ctttttgcca ggcgtttttc
     9721 gtcaggctat agctgagcgt ccagttgata tcttcgaacg cagtatttaa tccagcctgg
     9781 aattgctcat cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt
     9841 gttgatgtgc gcccgagttg ctgagtaacg gtgagctgta atttcccgcg tttgttataa
     9901 gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa taactccatc ctgtgtttcg
     9961 atgttgtagc cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc
    10021 gaataacggt aacccactaa ctgaatattc gtgcctgatt cattgagcga tttgttatag
    10081 agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctgc
    10141 gtcatatcca cagacagagc gcccagtgcc tccatgtttt tcccgatacc gaaattaaaa
    10201 gcacgataac gatccgccag ttgcgttcca ccatatattg tccagccagc cggaaggccg
    10261 tggagtaatg tactctggaa aaagcggggt ttttcctgct gcgcatttcc actacggtat
    10321 tctcctgccg taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa
    10381 tagggtacgg taaaaatctg cgtgctgccg tcagcctctt tgatcgttac ctgcaagtca
    10441 ccactattac ctgcggcata gatatcgttg atggtaaaag gccccggtgg caccgtacta
    10501 ttataaatgc catatccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg
    10561 tggatcaccg gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat
    10621 tgtgcgccgc gaaagttaat accatcgaaa atatcgccct gggtataacc atcacccaac
    10681 gtcagccggg aacgtaaagg aattatgtct cgctcaatcc aggtaatgat atgctgccat
    10741 ttatttttgc tacctgatga actgtcgcta ctgttataac tccaggtggt attgtcgcgt
    10801 aaacgccacg caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta
    10861 cccccaatcc
//
LOCUS       DAZSNG010000124        10755 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZSNG010000124 DAZSNG010000000
VERSION     DAZSNG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.125"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            85..2337
                     /locus_tag="ACHW4Z_003441"
     CDS             85..2337
                     /locus_tag="ACHW4Z_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8882931.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            2673..3605
                     /gene="glaH"
                     /locus_tag="ACHW4Z_003442"
     CDS             2673..3605
                     /gene="glaH"
                     /locus_tag="ACHW4Z_003442"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR8882932.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3625..4893
                     /gene="lhgO"
                     /locus_tag="ACHW4Z_003443"
     CDS             3625..4893
                     /gene="lhgO"
                     /locus_tag="ACHW4Z_003443"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR8882933.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4916..6364
                     /gene="gabD"
                     /locus_tag="ACHW4Z_003444"
                     /pseudo
     CDS             4916..6364
                     /gene="gabD"
                     /locus_tag="ACHW4Z_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6372..7658
                     /gene="gabT"
                     /locus_tag="ACHW4Z_003445"
                     /pseudo
     CDS             6372..7658
                     /gene="gabT"
                     /locus_tag="ACHW4Z_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7896..9296
                     /gene="gabP"
                     /locus_tag="ACHW4Z_003446"
     CDS             7896..9296
                     /gene="gabP"
                     /locus_tag="ACHW4Z_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR8882934.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9317..9979
                     /gene="csiR"
                     /locus_tag="ACHW4Z_003447"
     CDS             9317..9979
                     /gene="csiR"
                     /locus_tag="ACHW4Z_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR8882935.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9980..10429)
                     /gene="kbp"
                     /locus_tag="ACHW4Z_003448"
     CDS             complement(9980..10429)
                     /gene="kbp"
                     /locus_tag="ACHW4Z_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR8882936.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10513..10671)
                     /locus_tag="ACHW4Z_003449"
     CDS             complement(10513..10671)
                     /locus_tag="ACHW4Z_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR8882937.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2724 a         2660 c         2754 g         2617 t
ORIGIN      
        1 ctgtttgcaa ttactttcac aacaattaaa catttttatg ttttcgtata catcatatta
       61 ctctaccatt agaggaacgt tattatgttt tctataaaac caggacctgg aaatttacct
      121 atcgacaacc ccacattgtt atcatggaac attactgatg gggatctaaa ttccaaattt
      181 aatacattag aatatctaaa ctgtataaca aatattatta atgcttgtgg agtttatcct
      241 caggatttaa aagacagaga aattatatca acttttcacg cagaaaaagt cattaatgat
      301 ctgttaaaaa acgattataa aatttccctt tctccagata caacttaccg agagctgaat
      361 aaagctgcac agagtagcat tacagcgcca gacaggatag gagaagggaa aacatgggtt
      421 tatcaacgag atacaatggt tgaaagaggt gataacagtg gtgttcatca gtatggtcca
      481 gctgaacatt tcactcacat tatatctgac aaaccgtccc caaaagataa gtatgttgca
      541 tatgctatta acattcctga ctatgagcta gcagccgatg tatataatat taacgtgacg
      601 tcaccttccg gacagcaaga aacatttaaa atactaatca atccagaaca tctacggcaa
      661 acacttgagc gtaaatctct tactgctgtt cagaaatcac aatgtgaaat catcaccccc
      721 aaaaaacctg gcgaagcgat tcttcatgct tttaatgcca cctaccagca aatcagggaa
      781 aatatgtctg agtttgcacg ttcccattat gggtatatac aaatccctcc agtgacaact
      841 ttccgcgccg acggaccaga aactcccgaa gaagaaaaag gttactggtt tcacgcttat
      901 caacccgaag atctttgtac catccataat ccaatggggg atttgcagga ttttatcgca
      961 ttggttaaag atgctaaaaa atttggtatc gatatcattc ctgattatac ctttaacttt
     1021 atgggaatcg ggggaagtgg taaaaatgac ctggattatc cctctgctga tatacgagcg
     1081 aagatcagta aagatataga aagtggtatc cctggctatt ggcaaggtca ggttttgatt
     1141 ccattcatta aagatccagt aacaaaagaa cgtaaacaaa tccatccaga agatatacat
     1201 ctcactgcaa aagacttcga agcaagtaaa gataacatct ctaaggatga atggaaaaac
     1261 ctccatgcat taaaagaaaa gagtttaaat ggaatgccta aaacaacccc caaaagtgac
     1321 caggttatta tgttgcaaaa tcaatacgtt cgtgaaatgc gaaaatatgg cgtacgaggt
     1381 ttacgttatg atgcagcaaa acactcaaaa catgaacaaa tagaaagatc aataacccca
     1441 ccgcttaaaa attataatga gcgattacac aatactaact tatttaaccc aaaatatcat
     1501 gaaaaaaccg ttatgaatta catggaatat ctggtaactt gtcagttgaa tgaagaacaa
     1561 atgtcatcgc tgctttatga aagagatgat ttaagcgcca ttgatttttc attgctcatg
     1621 aagacgataa aagccttttc attcggggga gatctcctaa cccttgcatc aaaaccgggt
     1681 tccacaatct caagcatccc atcagaaaga cggatattga ttaacattaa ccacgatttt
     1741 cctaacaatg gcaatctttt caatgacttt ctatttaacc atcaacaaga tgaacaatta
     1801 gcaatggcat atatggccgc tctcccgttc agcaggcctt tagtttactg ggatggccag
     1861 gtattaaaat caacgactga aattaaaaat tatgatgggc ccacgcgtgt cggtggtgag
     1921 gcgtggctta ataaaggttg ctctacctat cagcagctct acaatgaatt ccacgcatta
     1981 tatatagata aagcaggaat atggagcgca tttgagggtg tatttgcaac taaaaacgtt
     2041 ctggccttta gtcgtgggga ttctgtgaac attaatcact ctcctcatga tggactagtt
     2101 ataataaata aaggaaacga agaagttgaa ggtacctggc ctaacaaatt gcaacctgga
     2161 atatataaaa acatggggag taatagcgtt aacattatta ttaataatac ccgaaaaatt
     2221 atcccccctt gtaaagtatt tacgcttaga ggcggatctc taaatatcaa tattcctagg
     2281 cgtagcgctc ttcttttagg gaaaacagga gaaccgccga actatctcta tttataattt
     2341 aatgttatat ctgccccgat aaaacggggc agataatatg tttagtttac taacggtcat
     2401 tttgtagtga agccatttac tgttttttat cgaccagata atctgttctc taatgttaac
     2461 tccccctaac ctgttgcttt agttattcat ttcctgtctc actttgcctt aataccctac
     2521 gttaaatgtt actaatttgt tgcttttgat cacaataaga aagcaatatg tcgcttttgt
     2581 gcgcattttt cagaaatgta gatattttta gattatggct acgaaatgag catcgccatg
     2641 tcaccctaca tctcataaga ggatcgcttc tgatgaatgc actgaccgcc gtacaaaata
     2701 acgctgtcga ttcaggccag gactatagcg gattcactct catcccgtcg gcgcaatccc
     2761 cgcgtctgct ggaactcacc ttcaccgaac agacgaccaa acagtttctc gaacaggttg
     2821 ccgaatggcc cgtgcaggcg ctggagtaca aatcgtttct gcgttttcgg gtaggcaaaa
     2881 ttcttgacga tctgtgtgcg aatcagctgc aaccattgct gttgaagacc ctgctaaacc
     2941 gtgctgaagg tgcgctgttg atcaatgcgg taggtatcga tgatgtggcg caggcagatg
     3001 agatggtgaa gctggcaacg gcggtagcgc atctgattgg tcgctcaaat ttcgatgcga
     3061 tgagcggtca gtattacgcg cgtttcgtgg tgaaaaatgt cgataactca gacagctatc
     3121 tgcgtcagcc gcaccgcgtg atggaactgc acaacgacgg cacgtatgtc gaagagatca
     3181 ccgattacgt gctgatgatg aaaatcgatg actgggaaca tctggaccac tatttccgcc
     3241 atccactggc gcgtcgcccg atgcgctttg ccgcgccgcc gagcaaaaac gtcagcaaag
     3301 atgttttcca tccagtgttc gacgttgatc aacagggtcg cccggtgatg cgctatatcg
     3361 accagttcgt ccagccaaaa gattttgaag aaggcgtgtg gttgagcgag ctttccgatg
     3421 ccattgaaac cagcaaaggc attctttctg tacccgttcc cgttggcaaa ttcctgttga
     3481 taaacaacct gttctggctg cacggtcgcg accgctttac tccgcacccg gatctgcgcc
     3541 gtgaactgat gcgtcagcgt ggctatttcg cttacgccac tcaccactac cagacgcatc
     3601 agtaagcgca aaggaattga gcggatgtat gattttgtga ttattggcgg cggcatcatc
     3661 ggcatgtcga ccgccatgca actgattgat gtttatccgg atgcccgcat tgcgttgctg
     3721 gaaaaagagt ccggcccggc ctgtcaccag acgggccaca acagcggcgt gatccatgcc
     3781 ggggtctatt acacgcccgg cagcctgaag gcgcagtttt gcctggcggg gaaccgtgcc
     3841 accaaagcct tttgcgatca aaacggcatt cgctatgaca actgcggcaa gatgctggtc
     3901 gccacgtctc agctcgaaat ggaacggatg cgcgccttat gggaacgcac agcggcgaac
     3961 ggtatcgagc gcgagtggat aaacgccgtg gaactgcgcg agcgcgaacc gaatatcacc
     4021 gggcttggtg gcatttttgt gccgtccagc ggcattgtca gctaccgcga agtgacggcg
     4081 gcaatggcaa aaatcttcca ggccagaggc ggcgagatta tttataacgc cgaagtcagc
     4141 gccctcagtg agcataaaaa cggcgtggtg atacgcaccc gtcagggcgg tgaatatgaa
     4201 gcatcgacgc tgattagctg ttccgggctg atggctgacc ggctggtgaa aatgctcggc
     4261 ctcgaaccgg gctttattat ctgcccgttc cgtggcgagt atttccgcct tgcgccggag
     4321 cataaccaga ttgttaacca cctgatttac cccattcccg accccgcgat gccatttttg
     4381 ggcgtgcatc tcacccgcat gatcgacggc agcgtgaccg tcgggccaaa cgcggtgctg
     4441 gctttcaaac gcgaaggcta tcgcaagcgc gacttctcat ttagtgacac gctggagatt
     4501 ttgagctcgt cggggattcg ccgggtgctg caaaaccatc tacgctcagg actgggcgag
     4561 atgaaaaact cgctgtgcaa aagcggctat ctgcggctgg tgcaaaagta ttgtccccgg
     4621 ctttcgttaa gcgatctcca gccctggccc gccggtgtgc gggcgcaggc agtatcgccg
     4681 gacggcaagc tgattgacga ttttctgttt gtcaccaccc cacgcacgat ccacacctgc
     4741 aatgcgccct ccccggcagc gacatcagca attcctattg gtgcgcatat tgtcagcaag
     4801 gtacaaacgc tgttggcaag ccagagtaac cccggacgca cgctgcgagc ggcacgtagt
     4861 gtggatgcct tacacgccgc atttaatcaa taacctttga aaacaggacg taacaatgaa
     4921 acttaacgac agtaacttat tccgccagca ggcgttgatt aacggggaat ggctggacgc
     4981 caacaatggt gaagtcatcg acgtcaccaa tccggcgaac ggcgacaagc tgggtagcgt
     5041 gccgaaaatg ggcgcagatg aaacccgcgc cgctatcgac gccgccaacc gcgccctgcc
     5101 cgcctggcgt gcgctcaccg ccaaagaacg cgccaccatt ctgcgcaact ggttcaattt
     5161 gatgatgcag catcaggacg atttagcgcg cctgatgacc ctcgaacagg gtaaaccgct
     5221 ggctgaagcg aaaggcgaaa tcagctacgc cgcctccttc attgagtggt ttgctgaaga
     5281 aggcaaacgt atttatggcg acaccattcc tggtcatcag gccgataagc gcctgattgt
     5341 tatcaagcag ccgattggcg tcaccgccgc catcacgccg tggaactttc cggcggcgat
     5401 gattacccgt aaagccggtc cggcgctggc ggcaggctgc accatggtgc tgaagcccgc
     5461 cagtcagacg ccgttctctg cgctggcgct ggcggaactg gcgattcgcg cgggcattcc
     5521 ggctggcgtg tttaacgtgg tcaccggttc ggcaggcgtg gtcggtaatg aactgaccag
     5581 caacccgctg gtacgcaaac tgtcgtttac cggttagacc gagattggcc gccagttaat
     5641 ggaacagtgc gcgaaagaca tcaaaaaagt gtcgctggag ctgggcggca acgcgccgtt
     5701 tatcgtcttt gacgatgccg atctcgataa agccgtggaa ggcgcgctgg cctcgaaatt
     5761 ccgcaacgcc gggcaaacct gcgtctgcgc caaccgttta tacgtgcagg acggcgtgta
     5821 tgaccgcttt gccgaaaaat tacagcaggc ggggagcaaa ctgcacatcg gcgacgggct
     5881 ggagaaaggc gtcaccatcg ggccgctgat cgatgaaaaa gcggtagcaa aagtggaaga
     5941 gcatattgcc gatgcgctgg agaaaggtgc gcgtgtggtt tgcggcggta aagcgcacga
     6001 acgcggcggc aacttcttcc agccaaccat tctggtggac gttccggcca acgccaaagt
     6061 gtcgaaagaa gagacgttcg gccccctcgc cccactgttc cgttttaaag atgaagccga
     6121 tgtgattgca caagccaatg acaccgagtt tggtcttgcc gcctatttct acgcccgtga
     6181 tttaagccgc gtcttccgcg tcggcgaggc gctggagtac ggcatcgtcg gcatcaatac
     6241 cggcattatt tccaatgaag tggccccatt cggcggcatc aaagcctcgg gtctgggtcg
     6301 tgaaggttcg aagtatggca tcgaagatta cttagaaatc aaatatatgt gcatcggtct
     6361 ttaactggag aatgcgaagg aacagcaata aagagttaat gcagcgccgc agtcaggcga
     6421 ttccccgtgg cgttgggcaa attcacccga ttttcgctga ccgcgcggaa aactgccggg
     6481 tgtgggacgt ttaaggccgt gagtatcttg atttcgcggg cgggattgcg gtgctcaata
     6541 ccgggcacct gcatccgaag gtggtggccg cggtggaagc gcagttgaaa aaactgtcgc
     6601 acacctgctt ccaggtgctg gcttacgagc cgtatctgga gctgtgcgag attatgaatc
     6661 agaaggtgcc gggcgatttc gccaagaaaa cgctgctggt tacgaccggt tccgaagcgg
     6721 tggaaaacgc ggtaaaaatc gcccgcgccg ccaccaaacg tagcggcacc atcgctttta
     6781 gcggcgagta tcacgggcgc acgcattaca cgctggcgct gaccggcaag gtgaatccgt
     6841 actctgcggg catggggctg atgccgggtc atgtttatcg cgcgctttat ccttgcccgc
     6901 tgcacggcat aagcgaggat gacgctatcg ccagcatcca ccggatcttc aaaaatgatg
     6961 ccgcgccgga agatatcgcc gccatcatga ttgagccggt tcagggcgaa ggcggtttct
     7021 acgccgcgtc gccagccttt atgcagcgtt tacgcgctct gtgtgacgag cacgggatca
     7081 tgctgattgc cgatgaagtg cagagcggcg cggggcgtac cggcacgctg tttgcgatgg
     7141 agcagatggg cgtggcgccg gatcttacca cctttgcgaa atcgatcgca ggcggtttcc
     7201 cgctggcggg cgtcaccggg cgcgcggaag tgatggacgc tgttgcaccg ggcggtctgg
     7261 gcggcaccta tgccggtaac ccgattgcct gcgtggctgc gctggaagtg ttgaaggtgt
     7321 tcgagcagga aaatctgctg caaaaagcca acgatctggg gcagaagcta aaagacggat
     7381 tgttggcgat agccgaaaaa cacccggaga tcggcgacgt acgcgggctg ggatcgatga
     7441 ttgccatcga gctgtttgaa gatggcgatc acagcaaacc ggacgcaaaa ctcaccgccg
     7501 agatcgtggc acgcgcccgc gataagggcc tgattcttct ctcctgcggc ccgtattaca
     7561 acgtgctgcg catccttgta ccactcacca ttgaagacgc tcagatccgt cagggtctgg
     7621 agattatcgg tcagtgtttt gatgaggcga agcagtagca ccgtccccat gcaggaggcg
     7681 acgctgcgcg tcttgtccgg cctacgagaa tccaagtcgg ataaggcgtt tacgccgcat
     7741 ccgacaatct gtacgtgaac aggaataaat ctatgtcggc cggataaggc ggagccgctc
     7801 tccggcaaaa agaatcaata acaattaaac gcgtgacccg acgcgggaaa tgtcggggcg
     7861 ctctcccaag tgacacactt tcgagaggat tcaggatggg gcaatcatcg caaccacacg
     7921 agttaggcgg cgggctgaaa tcacgccacg tcaccatgtt gtctattgcc ggtgttatcg
     7981 gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga agcggacccg gcggtattac
     8041 tggcctatct gttcgccgga ttactggtgg ttatgattat gcggatgctg gcggaaatgg
     8101 cagttgccac gcccgatacc ggttcgtttt ccacctatgc cgataaagcc attggccgct
     8161 gggcgggcta taccatcggc tggttgtact ggtggttttg ggtactggtt atcccactgg
     8221 aagccaacat cgccgccatg atcctgcact cgtgggttcc aggcattccc atctggttat
     8281 tttccctcgt cattaccctc gccttaactg gcagtaattt attaagcgtt aaaaactacg
     8341 gcgaatttga gttctggctg gcgctgtgca aagtcatcgc tatcctggcc tttattttcc
     8401 ttggtgcagt cgcaattagc ggtttttacc cttatgccga agtgagcggg atctcaagat
     8461 tgtgggatag cggcggcttt atgcccaacg gtttcggtgc ggtattaagc gcgatgttga
     8521 tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat tgccgccgcg gaatccgaca
     8581 tgccggaaaa acatattgtc cgcgccacca actcggttat ctggcgtatt tctatcttct
     8641 atttgtgctc tatttttgtc gtcgtggcat taataccgtg gaatatgccg gggctgaaag
     8701 ccgttggatc ttatcgctcg gttctggaat tgctcaatat tccccatgcg aaattaatca
     8761 tggactgtgt gatattactt tccgtaacca gttgcctgaa ctcggccctg tataccgcct
     8821 caaggatgct ctactcctta agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca
     8881 accgcagtaa aaccccgtac gtggcggtgt tactctccac cggcgcagca ttcttaacgg
     8941 tggtggtgaa ctattacgcg cctgcgaagg tatttaaatt tctgatcgac agctccggcg
     9001 ctatcgccct gctggtttat ttagtcatcg ccgtttcaca gttgcggatg cgcaaaattc
     9061 tgcgagcaga aggaagcgaa attcgcttgc gaatgtggct tgatccctgg ctcacctggc
     9121 tggtcatcgg ctttattacc tttgtgttgg tagtgatgct attccgtccg gcgcaacagt
     9181 tagaagtgat ctccaccggc ttattagcga tagggattat ctgtaccgtg ccaattatgg
     9241 ctcgctggaa aaagctggta ttgtggcaaa aaacacccgt tcataatacg cgctgataat
     9301 gatcaggagt cacaccatga ccattacgtc tctggatggc tatcgctggc tgaagaacga
     9361 tattattcgc ggtaattttc aaccggatga aaaattacgg atgagtttac tgacatcgcg
     9421 ttatgcactt ggcgttgggc cgttgcagga agcactttct caactggtgg cggaacggct
     9481 ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct atgtcagagc aggagctgct
     9541 cgatattttc gacgcccgcg ccaatatgga agcgatgctg gtcagtctgg cgattgcccg
     9601 cggtggcgat gaatgggagg cagacgttct cgcaaaagcg catctgctca gtaagctgga
     9661 agcctgtgac gccagcgaga aaatgcttga tgagtgggat ctgcgtcatc aggcgtttca
     9721 tacagcaatt gtggcgggct gtggttctca ctatttgctg caaatgcgtg aacggttgtt
     9781 tgatctggcg gcgcgttatc gatttatctg gctgcggcga acggtgcttt cggtggaaat
     9841 gctggaggat aaacacgatc agcaccagac cctgactgcg gcggtactgc cgcgagatac
     9901 agcgcgcgcc agtgagttaa tgcgccagca tttactgacg ccaattccca ttatccagca
     9961 ggcgatggct ggcaattaac tactcttccg gaatacgcaa cacttgcccc ggataaattt
    10021 tatccgggct ttttagcatc ggtttattcg cttcgaagat tttattgtac agattagcgt
    10081 taccgtagac ctgtttggaa atggcactca gagtgtcgcc agacttaacg gtataaaact
    10141 ggctggcagt ggctggtgtc gccgttttca cctgatcatc aacactggca ataccggaaa
    10201 tattccccac cgcaacaagg attttctcct tcgcctcctg actcaggccg tcaccagtga
    10261 ccgtcgcttt gccgtcggca atttgaatgt tcactttatc ggcatcaggt ataccggttt
    10321 tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt atcgtgctga cctgtaaccg
    10381 catcccagag tttttctccg gcatctttaa caaaattgaa cagacccata gctacctcat
    10441 tcgttaacgg aaacactcag aaagtgtagc agaggaacgc ggcgcgttaa cgccgcgggg
    10501 gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg tgaatcaaac cggggatata
    10561 cccaagcagt gtcagcagaa tattaataat gaacgcccaa ccgaaccctt taccgagcag
    10621 cacgccgatc ggcggcagaa tgatggtgat gacgattctc cagaaaccca tatgtactcc
    10681 ctataagaaa attactcatt gtttaaaaag agattttatc tcttaagcgt agtaatttta
    10741 gcggagcctg ccagt
//
LOCUS       DAZSNG010000125        10677 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZSNG010000125 DAZSNG010000000
VERSION     DAZSNG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.126"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1816)
                     /gene="selB"
                     /locus_tag="ACHW4Z_003450"
     CDS             complement(<1..1816)
                     /gene="selB"
                     /locus_tag="ACHW4Z_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR8882938.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLL"
     gene            complement(1813..3204)
                     /gene="selA"
                     /locus_tag="ACHW4Z_003451"
     CDS             complement(1813..3204)
                     /gene="selA"
                     /locus_tag="ACHW4Z_003451"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR8882939.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3302..3910)
                     /locus_tag="ACHW4Z_003452"
     CDS             complement(3302..3910)
                     /locus_tag="ACHW4Z_003452"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR8882940.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3981..5066)
                     /locus_tag="ACHW4Z_003453"
     CDS             complement(3981..5066)
                     /locus_tag="ACHW4Z_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8882941.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5069..5431)
                     /locus_tag="ACHW4Z_003454"
     CDS             complement(5069..5431)
                     /locus_tag="ACHW4Z_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8882942.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5968..7881
                     /gene="mtlA"
                     /locus_tag="ACHW4Z_003455"
     CDS             5968..7881
                     /gene="mtlA"
                     /locus_tag="ACHW4Z_003455"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR8882943.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7976..9124
                     /gene="mtlD"
                     /locus_tag="ACHW4Z_003456"
     CDS             7976..9124
                     /gene="mtlD"
                     /locus_tag="ACHW4Z_003456"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8882944.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9124..9711
                     /gene="mtlR"
                     /locus_tag="ACHW4Z_003457"
     CDS             9124..9711
                     /gene="mtlR"
                     /locus_tag="ACHW4Z_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR8882945.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9723..9932)
                     /gene="yibT"
                     /locus_tag="ACHW4Z_003458"
     CDS             complement(9723..9932)
                     /gene="yibT"
                     /locus_tag="ACHW4Z_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR8882946.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10216..10578
                     /locus_tag="ACHW4Z_003459"
     CDS             10216..10578
                     /locus_tag="ACHW4Z_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR8882947.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2568 a         2883 c         2753 g         2473 t
ORIGIN      
        1 gcaataaatg atcatttcca cgacgacgcg taaagccaat gcggtcaaaa tattccagaa
       61 tctgaattgc cagctttcgg cctacgccta agcgatcgcg gaaatccgcc gcgcaggttg
      121 aaccacactc ctgatcgaga tcgcggatca tattggcaaa ttcgacaatc cgatcgttac
      181 ggtaataacg atctttgacg atcgcggtaa ttattccttg ctgcgccgcc tggcgtagcg
      241 tcaggcgcat tgcctgctcg tcggtacccg tctcttttgc cagatcacgc acccaccacg
      301 gctcgtcacc aaacagcggc tctgcttttt gccagatggc ctgctgctct tcgctgaagc
      361 ccgctttgtg atccggcaga tgcagccaac cgtgatgact gaggatgtcg ccgctttcgc
      421 gcatcttttc aatcagcaac agtactagcg cttcatcttc cattggcaac gccatacgtc
      481 gcagacgttc gcgcccaggc ccaggttcat cgcgatgttg ctcatgataa gtcgctaatg
      541 tgtcgagaat tttccgttgc cagcgagcgg caaccggcgc attcaacaag ctataaccag
      601 cctgaatata gccaggctgt tgcagcagtt cgcgcatccc ttcgccgttg agctggcgcg
      661 cccaagcgaa atcaggaaga ttaaccgcgc cgcgttgcag gtgaacggct aacgcttcgc
      721 tatcattctg tgccctggcg agggaagcca gccattgcag atactctggt ttacgtttac
      781 cgcgacgcgg cgggttaagc atcacgacgc gcgctccggc cagcgtgttg cgggcagaga
      841 tatcgcgcaa taccaggcgg tcgttatctg ccagccataa cggggtgtcg aagaccagtt
      901 cagcaaggtt atcttccagc agtgaaacgc gtcccgtgac gtggctggcg gcgtggtgaa
      961 tatgcagcgg ctgccactgg gtcagcggtg catgggtttg aagctcgaca atcacccgtg
     1021 tgaacggctc tggcggcaca tcggcaagca gccagtcgcc acggttaatc tgctcttttt
     1081 ccgcatcacc cgcgatgtca agcgcgatac gctgcccggc attggcggtt tctgttggct
     1141 ggttttgcgc atgcagcgca cgtacacgca tcggtttatt tacaccagtc agccagagtg
     1201 aatcgcctac cttcacttcc ccgcttaacg ccgtaccggt gacgaccagc ccggcacctt
     1261 ttacggtaaa tgcgcggtca atcgcgaggc ggaaactatg ttggctggcg tgctcgcgtt
     1321 ccggcaactg aagcagatgc tcgcgcaggg catcaattcc ccgaccttcg gttgctgcgg
     1381 tgataaacag ttttgcctca gcaaaaccgt attcccgcag aacctccttt acctggcgtt
     1441 caacctcatc aacacgcgct tcgtccacgc gatcggcttt ggtcagcgcc actgtcagca
     1501 tcgggttacc ggtcagctgc aaaatcgcca gatgctcacg ggtctgtgcc atcacgccgt
     1561 catcgcacgc caccaccaac agcgcgtgat cgataccacc aacgcccgcc agcatgttgg
     1621 aaagaaactt ttcatgaccg ggaacgtcga taaaaccagg cacgcgacca tccggctgcg
     1681 gccagtaggc atagccgaga tcgatggtca tgccgcgctt tttttcttcc ggcagacggt
     1741 cagcatttac gccagtaatc gcctgcaata aggttgtttt gccgtggtca acgtgtccgg
     1801 cagtcgcaat aatcatttca acaacatctc caaaaaccgt tgctcatctt caaggcagcg
     1861 taaatccagc cacaatcgtc cgtcataaat acgaccaatc accggcactg gcaattcacg
     1921 ccagcgggcg gctaatgact caaggtggct accgcgtcca tcatggggtg taaacgttaa
     1981 tgccgcgctc ggcaggcgat caaccggcag cgaaccactg ccaatctgcg aaagacatgg
     2041 cataacctgt accgcaaact ccgcgccgta atgtgcgaca aggggagcct gtaaacgttg
     2101 tgcctggatt tgaatgacct ctgcgctgcg ggtaagcagg cgcagggtcg gtaatttttc
     2161 actcagagct tcagggtgta aataaagacg caacgtggct tccagcgccg cgagggtcat
     2221 tttatccgcg cgtaatgcac gcttcagcgg gtggctttgc aggcgggcga tcatctcttt
     2281 tttaccaacg ataatcccgg cctgcggccc gcctaacaac ttgtcgccgg agaagctcac
     2341 cagactgacg cccgccgcaa tcaactcctg cggcattggc tctttcggca aaccgtactg
     2401 gctaagatcg accagcgagc cactgcctaa atcagtcacc actggaacat ccagctcttt
     2461 gccgagcgcc accagttcag cttcgtctat cgctttggtg aacccctgaa tgctgtagtt
     2521 actggtatgt actttcatca acagtgcggt attttcattc accgcctgac gataatcatt
     2581 cgcgtgcgtg cggttggtgg tccctacttc gtgtagggtg cagcctgcct gacgcataac
     2641 atcgggaata cgaaacgcgc cgccaatctc caccagttcg ccgcgagata ccaccacctc
     2701 tttcccgctg gcagtggccg ccaacatcaa taacaccgcc gccgcattgt tattgacgat
     2761 acaggcatct tccgcccccg taatacggca cagcagctgc gccagcgccc gatcgcgatg
     2821 tccgcgtccg gcgtcgtcca gatcgtactc gagggtcact ggcgaacgca tagcctgcgc
     2881 aacggcttcc accgcggctt ccgcctgtaa agctcgccca aggttggtat gcagcacggt
     2941 tcccgtcagg ttgatcaccg gacgcagcgc gctctgcgcc tctttcgtca accgggcatc
     3001 gacttcttgc gcccagtttt cacaccacgc aggcagcgtc tggctgccac gaatcacttc
     3061 tcgcgcttcg tcgagcatct gacgcaacaa ttccaccacg cgggtgtgac cataagtatc
     3121 acgcaaagaa aggaaggagc tatcgcgcaa taagcgatca atagccggaa gttgactata
     3181 gagggaacgc gtttcggttg tcataggaaa cctggctgat caaggccctc tcacacggag
     3241 aagggcgttt aacataacca cggattgtaa cgtgagatgg gtcaggagga catatcgcgc
     3301 atcaagcctt tggcggttcg gtgcgagcaa aactttcgcg gctaaacagg ttttcgacca
     3361 gtttgactaa gtgtgggcgg tcgacacacc aacctggcgc aacgcggcgg aaattgagat
     3421 atccgacagc acaggcaatg gcgatagtcg ccagattgac cgtatcggtt ttgagtgtgc
     3481 catcgaccag atatccttcc agcacatcca gactgcggtt gattttctcc cgctggcgta
     3541 acaattcatc ttcagactgc tgcgccgccg gacgcgcctg ttcacgcacc gataccagcc
     3601 cggcatccat aatgccatcc gccagcgcct caattttgcg cacccgcaac gactccagcg
     3661 gatcgcgcgg caacatcgcc ggagcgacat tcattaactc aatatattca gcgatgatcg
     3721 gcgaatcaaa ccagcattcg ccctcttcgg tcaccagcac cggcactttc cctaacgggt
     3781 taaattgcgc cacgccgtta tccgcgttat agggcagttc attaataaat tcgaaagtta
     3841 tgcccttttc taacaacaga ataaaaagtt tgcgtacaaa cgggctggtg tagctaccga
     3901 cgagtttcat gccgagtcct ttgtgcgagg aaaaatatca gtatggctca ggctcacgca
     3961 aaacacaggt gatataagtc tcaatgatcc aaataaagat aatgcatcca actggtcatt
     4021 cttagcagca ctttccgcat caccgaaaca tggctgaaat ggtcggagta aaccgccacc
     4081 tgggcgtaga tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt
     4141 cccagcacac cgtccgtgcc gggaacgacc gttaatgatt gcaatacccc ctgcgcctga
     4201 taagaaccgc ctggcacgac aggtaaaata ctagtcaatt tgccgtggaa cacctgccca
     4261 ggtagcgcgt taaacaccac ttccgcatca tcgccaggtt tcagacgtaa cagcgagttt
     4321 tgccgaaatt gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc
     4381 agcggcaagg cagctgcgta tgtacctggg cggatcagta cctgagtgac gtagccattg
     4441 cttggtgcgc gaatgacagt ctgctcaaga ttatattttg cttcagtaag ttgcgctctt
     4501 aagctcacaa tctgcgattg ctcgccgttg accatactgt cgagctgact ctggatctgc
     4561 gcctgctccg ccaccgagcc tttcactaac gcatcctgcg cgaggaaatt ttgccgcgca
     4621 tcgtcgatgt cacgttccga gaacggattc accgccgcct ggctgccttt caggtaacgt
     4681 tgataatttt taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct
     4741 tcagtgagct gcgcacgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga
     4801 agtctgtcaa ctcgcgcctg gtaacgaaca gggtcgagct taaaaagcac ctcgcccttt
     4861 tgaataagct gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg
     4921 atagggatcg ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc
     4981 aacaaaatca aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgccacg
     5041 taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa tagtcagtct
     5101 ttttctcggc ggcgagttga tgctccagcc gggaaattcg gtcagaaagt gcggcgattt
     5161 ccggttcagt cgctttctcc tgcgacgcaa catgtgactg cataccccag ccacgctccg
     5221 gttgatagag cgtcgcccag atccacagaa acggccagat aacatgcaga gtaaacaggc
     5281 tcacccagcc cgccgtatga atagcgtcgg catgggggtg attgcgcttt ttggcaatca
     5341 gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag acgaagatca
     5401 gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt ttatcagtgt
     5461 tgttttttgg ctggcagcca gaagggagtc aggctgatat tttgacaata atccgggttc
     5521 gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc ttagttacat
     5581 aacgattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac gccgtaagaa
     5641 aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa atgtgacatt
     5701 actcacattt aaatgccatt tttagcgaaa atcaccgcct tgtttctttt ttatacaggc
     5761 gttttgtgat gagcgtcacg tcaattacct ctctaccccc tatatttatg tgactgatat
     5821 cacacaaaag cccgtcgact ggacagttaa ccgattcagt gccagatttc gcagtatcta
     5881 caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg
     5941 acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa agtgcaaagc
     6001 tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt
     6061 atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct ggcgaagctg
     6121 gtcgggccga tgatcactta tctcctaccg ctgctgatcg gttataccgg tggtaagctg
     6181 gtaggcggca aacgtggtgg cgtagtcggt gccattacca ccatgggcgt tatcgtcggc
     6241 gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg ctggtgcatt
     6301 aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat gctggtgaat
     6361 aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct cggcattggc
     6421 ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat ggttgtccat
     6481 gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt cctcaacaac
     6541 gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga actgggtaaa
     6601 tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct gctggcgtac
     6661 atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat catccacttc
     6721 ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg tctgatcctc
     6781 gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg cggtggtctg
     6841 gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc aaaaggtgct
     6901 tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt tgtctctgct
     6961 attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc aactcgtcgt
     7021 atgcaggaca tgaaagctga gtctaaaggc acatctccgc tatctgcggg cgatgtgact
     7081 aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat gggttcaagt
     7141 gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc gcagatttcg
     7201 gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat cactcaccgt
     7261 gacctgaccg aacgcgctat gcgccaggtt ccgcaggcac agcatatttc gctgaccaac
     7321 ttcctcgaca gcggcctgta caccagccta accgaacgtc tcgttgctgc ccagcgccat
     7381 actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga ttccagtgct
     7441 aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc aaccaaagaa
     7501 gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga gccggaatac
     7561 gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga gtctatcgcg
     7621 gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt ggtgttctgc
     7681 cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg tctggtgatt
     7741 ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac caatgcactg
     7801 gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga agtgctggaa
     7861 ctgctggcag gtcgtaagta atccaatcct accctctcct cacggagagg gtttgggtga
     7921 gggaaaagcc tcaccccagc cctctcgggt aaaaacattg atgaaggtta atactatgaa
     7981 agcattacat tttggcgcag gtaatatcgg tcgtggcttt atcggtaaac tgctggcaga
     8041 cgcgggtatc caactgacgt ttgccgatgt caatcaggtg gtacttgatg ccctgaatgc
     8101 ccgtcatagc tatcaggttc atgtggtcgg tgaaaccgag caggtagata ccgtttccgg
     8161 cgtcaatgct gtcagcagca ttggtgatga tgtcgttgat ctgattgctc aggtcgattt
     8221 agtcactacc gccgttggcc cggttgtgct ggaacgtatt gctccggcta tcgccaaagg
     8281 gctggtgaaa cgcaaagaac aaggtaatga atccccgctg aacatcatcg cctgtgaaaa
     8341 catggtacgc ggcaccacgc agctgaaagg tcatgtgatg aacgccctgc cggaagacgc
     8401 caaagcgtgg gtagaagagc acgttggctt tgtcgattcc gccgttgacc gcatcgtacc
     8461 gccttcggct tcggcaacta acgatccgct ggaagtgacg gtagaaactt tcagcgaatg
     8521 gattgtcgat aaaacgcagt tcaaaggcgc actgccgaac atcccaggca tggagttaac
     8581 cgacaacctg atggcatttg tcgaacgtaa actcttcacc ctgaacacgg gtcatgctat
     8641 aaccgcgtac ctcggaaaac tggccggtca tcagaccatt cgtgacgcta ttctcgacga
     8701 gaaaatccgc gcggtggtaa aaggtgcgat ggaagaaagt ggtgcggtac tgatcaagcg
     8761 ctacggcttt gacgcagaca aacatgcggc gtacatccag aaaatcctcg gtcgttttga
     8821 gaacccgtat ctgaaagatg atgtagagcg cgtaggccgt cagccgctgc gtaaactgag
     8881 tgctggcgac cgtctgatca agccactgct cggtacgctg gaatacagcc ttccgcacaa
     8941 gaatctgatt caggggattg ctggtgcaat gcacttccgc agtgaagatg atccgcaggc
     9001 tcaggaactg gcagcactga tcgctgacaa aggtccgcag gcggcgctgg cacagatttc
     9061 cggtcttgat gccaacagcg aggttgtatc cgaggcggta accgcttata aagcaatgca
     9121 ataatggtgg accaggcgca ggacaccctg cgcccgaata acagattgtc agatatgcag
     9181 gcaacaatgg aacaaaccca ggccgttgaa aaccgtgtgc ttgagcgtct gaatgctggc
     9241 aaaaccgtgc gaagctttct gatcaccgcc gtcgagcttt tgaccgaggc ggtaaatctt
     9301 ctggtgcttc aggtattccg caaagacgat tacgcggtga agtatgctgt agaaccgtta
     9361 ctcgacggcg acggtccgct gggcgatctt tctgtgcgtt taaaactcat ttacgggttg
     9421 ggcgtcatta accgccagga atacgaagat gcggaactgc tgatggcatt gcgtgaagag
     9481 ctaaatcacg acggcaacga gtacgccttt accgacgacg aaatccttgg accctttggt
     9541 gaactgcact gcgtggcggc gttaccaccg ccgccacagt ttgaaccagc agactccagt
     9601 ttgtatgcaa tgcaaattca gcgctatcaa caggctgtgc gatcaacaat ggtcctttca
     9661 ctgactgagc tgatttccaa aatcagctta aaaaaagcct ttcaaaagta agcaacgtct
     9721 gcttactgcc cctctacctg cttcggccga taaagccgac gataatactc cagacgttgt
     9781 agatataacg gcacgctttc atcgggtatt ccggacggaa tcgcgttacg gggaggaagt
     9841 tttttcagat actcccggaa cgcctggctt gatgccatga aatctacggc tttatcgata
     9901 agaagcggaa cgttttcacc taatttgccc atgatgatat ctccgtatta ccccgcgccg
     9961 ggaatgcgcg gccgccaatt ttagtttagg ttcccggtaa agatgacatt aaggaaaacg
    10021 tgctgaatcc tcaataatca tccgctgctg tttattatct ttttctatag atttcttaat
    10081 taatcaacga attgcgtagt ttttgagcat gttaatgatt acgttgcaga actattacaa
    10141 tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt
    10201 atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaagcgtct cagcgatcgc
    10261 ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat
    10321 gccgagctgg aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgtgaggtt
    10381 catagccaaa aactgagtaa agaagcacag aagctgatga agatgccgtt ccagcgcgca
    10441 attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg
    10501 gttgtgcacc caatgaccgc gctggggcgc gaaatgggcc tggaagagat gactggattt
    10561 tctaagacta cgttttaagc ccctcagacg actggttcga tcgtatcaac ccgcatctgg
    10621 cttgccttag ctggtcaatg cggggactct cctgtctact cgcctaatta ccaaaca
//
LOCUS       DAZSNG010000126        10556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZSNG010000126 DAZSNG010000000
VERSION     DAZSNG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.127"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            38..778
                     /locus_tag="ACHW4Z_003460"
     CDS             38..778
                     /locus_tag="ACHW4Z_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR8882948.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHW4Z_003461"
     CDS             837..1223
                     /locus_tag="ACHW4Z_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR8882949.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHW4Z_003462"
     CDS             1232..1561
                     /locus_tag="ACHW4Z_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR8882950.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHW4Z_003463"
     CDS             1533..4574
                     /locus_tag="ACHW4Z_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR8882951.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHW4Z_003464"
     CDS             4574..4903
                     /locus_tag="ACHW4Z_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8882952.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHW4Z_003465"
     CDS             4903..5601
                     /locus_tag="ACHW4Z_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR8882953.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHW4Z_003466"
     CDS             5606..6349
                     /locus_tag="ACHW4Z_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR8882954.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHW4Z_003467"
     CDS             6313..6888
                     /locus_tag="ACHW4Z_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR8882955.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHW4Z_003468"
     CDS             6949..10428
                     /locus_tag="ACHW4Z_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8882956.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMYVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..>10556
                     /locus_tag="ACHW4Z_003469"
     CDS             10496..>10556
                     /locus_tag="ACHW4Z_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFR8882957.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2488 a         2498 c         3388 g         2182 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgtat
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgc
//
LOCUS       DAZSNG010000127        10465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZSNG010000127 DAZSNG010000000
VERSION     DAZSNG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.128"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="ACHW4Z_003470"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="ACHW4Z_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR8882958.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="ACHW4Z_003471"
     CDS             214..402
                     /locus_tag="ACHW4Z_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR8882959.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="ACHW4Z_003472"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="ACHW4Z_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR8882960.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="ACHW4Z_003473"
     CDS             597..770
                     /locus_tag="ACHW4Z_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR8882961.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="ACHW4Z_003474"
     CDS             complement(819..1892)
                     /locus_tag="ACHW4Z_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR8882962.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHW4Z_003475"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHW4Z_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="ACHW4Z_003476"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="ACHW4Z_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR8882963.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHW4Z_003477"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHW4Z_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR8882964.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="ACHW4Z_003478"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="ACHW4Z_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR8882965.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="ACHW4Z_003479"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="ACHW4Z_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10465
                     /gene="torD"
                     /locus_tag="ACHW4Z_003480"
     CDS             10277..>10465
                     /gene="torD"
                     /locus_tag="ACHW4Z_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8882966.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2685 c         2790 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tggctgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccacg
//
LOCUS       DAZSNG010000128        10399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZSNG010000128 DAZSNG010000000
VERSION     DAZSNG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.129"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHW4Z_003481"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHW4Z_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR8882967.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHW4Z_003482"
     CDS             complement(1433..1759)
                     /locus_tag="ACHW4Z_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR8882968.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHW4Z_003483"
     CDS             1894..2235
                     /locus_tag="ACHW4Z_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR8882969.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHW4Z_003484"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHW4Z_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHW4Z_003485"
     CDS             complement(2833..3543)
                     /locus_tag="ACHW4Z_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR8882970.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHW4Z_003486"
     CDS             3651..3956
                     /locus_tag="ACHW4Z_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR8882971.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHW4Z_003487"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHW4Z_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6299..7288)
                     /locus_tag="ACHW4Z_003488"
     CDS             complement(6299..7288)
                     /locus_tag="ACHW4Z_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882972.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="ACHW4Z_003489"
     CDS             complement(7373..7864)
                     /locus_tag="ACHW4Z_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882973.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="ACHW4Z_003490"
     CDS             complement(8185..8685)
                     /locus_tag="ACHW4Z_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882974.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="ACHW4Z_003491"
     CDS             complement(8675..9190)
                     /locus_tag="ACHW4Z_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882975.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="ACHW4Z_003492"
     CDS             complement(9621..9989)
                     /locus_tag="ACHW4Z_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882976.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         2677 a         2626 c         2569 g         2527 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga ggacatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acagaatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctcccta
//
LOCUS       DAZSNG010000129        10372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZSNG010000129 DAZSNG010000000
VERSION     DAZSNG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.130"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..338)
                     /locus_tag="ACHW4Z_003493"
     CDS             complement(<1..338)
                     /locus_tag="ACHW4Z_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8882977.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACHW4Z_003494"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACHW4Z_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACHW4Z_003495"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACHW4Z_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACHW4Z_003496"
     CDS             complement(1445..2008)
                     /locus_tag="ACHW4Z_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8882978.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="ACHW4Z_003497"
     CDS             complement(3383..5218)
                     /locus_tag="ACHW4Z_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8882979.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="ACHW4Z_003498"
     CDS             5319..5606
                     /locus_tag="ACHW4Z_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8882980.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="ACHW4Z_003499"
     CDS             5578..7107
                     /locus_tag="ACHW4Z_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFR8882981.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="ACHW4Z_003500"
     CDS             complement(7277..8494)
                     /locus_tag="ACHW4Z_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8882982.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /gene="ycdU"
                     /locus_tag="ACHW4Z_003501"
     CDS             complement(8861..9847)
                     /gene="ycdU"
                     /locus_tag="ACHW4Z_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFR8882983.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="ACHW4Z_003502"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="ACHW4Z_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3230 a         2371 c         1950 g         2821 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttagagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gc
//
LOCUS       DAZSNG010000130        10099 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZSNG010000130 DAZSNG010000000
VERSION     DAZSNG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.131"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            152..1281
                     /gene="ompN"
                     /locus_tag="ACHW4Z_003503"
                     /pseudo
     CDS             152..1281
                     /gene="ompN"
                     /locus_tag="ACHW4Z_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1422..1856
                     /gene="uspF"
                     /locus_tag="ACHW4Z_003504"
     CDS             1422..1856
                     /gene="uspF"
                     /locus_tag="ACHW4Z_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR8882984.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2032..2967
                     /gene="ttcA"
                     /locus_tag="ACHW4Z_003505"
     CDS             2032..2967
                     /gene="ttcA"
                     /locus_tag="ACHW4Z_003505"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR8882985.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3096..4463)
                     /gene="dbpA"
                     /locus_tag="ACHW4Z_003506"
     CDS             complement(3096..4463)
                     /gene="dbpA"
                     /locus_tag="ACHW4Z_003506"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR8882986.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(4493..4666)
                     /gene="ynaL"
                     /locus_tag="ACHW4Z_003507"
     CDS             complement(4493..4666)
                     /gene="ynaL"
                     /locus_tag="ACHW4Z_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR8882987.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4941..5924)
                     /gene="zntB"
                     /locus_tag="ACHW4Z_003508"
     CDS             complement(4941..5924)
                     /gene="zntB"
                     /locus_tag="ACHW4Z_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR8882988.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5938..5985)
                     /gene="ydaN"
                     /locus_tag="ACHW4Z_003509"
     CDS             complement(5938..5985)
                     /gene="ydaN"
                     /locus_tag="ACHW4Z_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR8882989.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6179..7411
                     /gene="dgcM"
                     /locus_tag="ACHW4Z_003510"
                     /pseudo
     CDS             6179..7411
                     /gene="dgcM"
                     /locus_tag="ACHW4Z_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(7432..7995)
                     /gene="smrA"
                     /locus_tag="ACHW4Z_003511"
                     /pseudo
     CDS             complement(7432..7995)
                     /gene="smrA"
                     /locus_tag="ACHW4Z_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(8249..9715)
                     /locus_tag="ACHW4Z_003512"
     CDS             complement(8249..9715)
                     /locus_tag="ACHW4Z_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR8882990.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(9712..>10099)
                     /locus_tag="ACHW4Z_003513"
     CDS             complement(9712..>10099)
                     /locus_tag="ACHW4Z_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8882991.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         2788 a         2511 c         2285 g         2515 t
ORIGIN      
        1 cttattatca tttcagagga attatttgat taaggtttta cttaaggcgt aacaaatgat
       61 atttatcggc taactgaact tctcctttag gatgttttca ctccccgaaa tggggataaa
      121 aggcaaataa aataactaaa ggatttattc aatgaaaaga aaagtactgg cacttttaat
      181 tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa
      241 caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa
      301 agatggcgac cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca
      361 acttactggc tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa
      421 aaaccagtca tggacccgtc tggcatttgc cggactgaaa tttgcagatt acggttcttt
      481 cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc
      541 tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc gagccaatgg
      601 cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt
      661 gcagtatcaa ggtaacaacg aaggagccag taatggtcag gaaggcacca ataacgaacg
      721 cgatgttcgt catgaaaacg gtgacggctg gggtctttcc acaacatatg atttaggcat
      781 gggctttagc gctggtgcgg catacacctc ttctgaccgc accaatgacc aggttaacca
      841 tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa
      901 caatatttac ctagcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag
      961 cgattatgca gttgcgaaca aaacccagaa ctttgaagtt actagtacca gtttgatttt
     1021 tgtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgaccttca cgctgctggt
     1081 ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc cgatgttggc
     1141 gcgacttact atttcaataa aaacatgtcc acctatgttg actataaaat caacctgttg
     1201 gatgaagatg acagtttcta cgctgccaac ggcatctcta ccgatgatat tgtcgcttta
     1261 ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt tttctgctta
     1321 ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa aaagtgtcat
     1381 ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga acgattcttg
     1441 tccctatcga tatttcagat tcagaattaa ctcaacgcgt gattagccac gttgaggcag
     1501 aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca ctgccctact
     1561 atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac ctgaaagcgg
     1621 aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc gacagagtgc
     1681 atgtccatgt tgaggaaggc tcgcccaaag accgcattct ggaattggcg aagaagatcc
     1741 ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat ctgctcggtt
     1801 ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg cgctgacact
     1861 aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg acattttccc
     1921 ctctaatccg taccatacac gccacagttt ttatatcaga tttcttatgc tgagcgttcc
     1981 ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta aatgtcgcaa
     2041 aatcaagaaa ttagtaagaa agaacaatac aacctgaaca aattacaaaa acgtctgcgt
     2101 cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg
     2161 gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg caatttgcag
     2221 caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc
     2281 ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta caagattgtt
     2341 gaagagaaca cttacggtat cgtgaaagag aagattccag agggcaaaac cacttgctca
     2401 ctgtgttctc ggctacgtcg cggtatcctt tatcgcaccg caacggaact ggggacgacg
     2461 aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc
     2521 tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc
     2581 gttattcgtc cactggccta ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa
     2641 gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg
     2701 attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga gacgatgttc
     2761 agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa
     2821 ggcattaccc acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag
     2881 atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt ggatgagtta
     2941 cggctgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg
     3001 ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atctggcaat cccgaataag
     3061 atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg cacccggcac
     3121 gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc aacagcctga
     3181 cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca
     3241 agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt ggcttttttc
     3301 ccgccatcga tacacaacgt tgccatttct gcttccagcg tcacaatgga actattagct
     3361 ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt
     3421 gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacga
     3481 ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca
     3541 agcgatttaa tatccagacc acgcgcagca acatcagtcg caaccagtac acgggcgcta
     3601 ccgttagcaa aacgtaccag ggtctgatcg cgttgctcca gatcgccgtg taacgacaat
     3661 gcactttgct ctacttcatt cagcgcgtcg cagacagcct ggcaatcttt tttggtattg
     3721 caaaacacca cacaagagga tggctgatgc aagcttaata accgttgcaa cagaggaatt
     3781 ttgcctttac tggatgtctc ataaaattgt tgttcaatgg gtggcaaagc atctgttgag
     3841 tcaatttcaa tcgccaaagg atcgcgttgc actcgtccgc tgattgcggc gatggcttcc
     3901 ggccaggttg ccgaaaacag aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca
     3961 tcaatggcat cgctaaatcc catatccagc atgcggtcgg cctcatccat caccaaagta
     4021 ttcagcgcat ccagtgatac cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt
     4081 gccacgataa tatgcggcgc atgttgcagc gaatcacgct gcataccgaa cggttgacca
     4141 ccgcacaacg tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg caattcacct
     4201 gccacctgat ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc
     4261 gacgcatcaa tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt
     4321 ttcgcctgca cgcgaacatc ttttccggca aggatcgccg gaagcgcggc ggcctgcacc
     4381 ggcgtcatgg ttaaataacc caactcatta aggttcgtga gttgggcggg aggcaaaaca
     4441 ttcagggtag aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa agttagcagg
     4501 cgcgtatcct cgcagatcta cgctcacgat gcgacaattt aatcggttct tcatcgggtg
     4561 gtgggtcagg catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag
     4621 gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc
     4681 attgggtgga ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca
     4741 tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga
     4801 caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaaggg agatattgcc
     4861 ctgaatgggt agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac
     4921 agccggaaaa attgcttttt ttacaaccat ttactacggt gcaaccataa agcaacacca
     4981 ccaataagaa caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcca
     5041 gggatcccac caaggttgac gccaaataac ccggtcagaa aggtactggg taaaaagacc
     5101 attgccatca acgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg
     5161 atttcatccg ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc
     5221 ccaaggcgat cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa
     5281 cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcaca
     5341 attaattgtt tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga
     5401 ttatcttcaa ggtcgataat tttatcgtgc agctgctcga taaattcact ggaatgatcg
     5461 gtcaacgcat cgcacacatc caccagccat cccccgcaat cggtcggacc cgtgccctct
     5521 tccagatcgc tcaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt
     5581 aaccgcccgt ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg
     5641 ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga aacggctgac tcggggccgt
     5701 gtgctctcgc ccgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc
     5761 cattgggcgc tatcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca
     5821 atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat
     5881 actgcatccg ggacattaac gtccgatccc ttaatcgctt ccactgtgcc tccatcatca
     5941 acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta
     6001 ccctgaaatg attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg
     6061 tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat
     6121 gacgtggctt ttttgcgatc ggatagcaac aaaaattgat aaaaataacg ggatctcaat
     6181 gattacgcac aacttcaata ccctggactt actcaccagt cctgtctgga ttgtttcgcc
     6241 ctttgaggaa cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac
     6301 gtttagtcag ctacgaaccg gaccctattc cgtctcctca caaaaagaac tgccgaaata
     6361 cctctccgat ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga
     6421 agaggaaaca gcattaagct gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt
     6481 gattattttc gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc
     6541 aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc
     6601 aatgttgttg attgacccgt cacgagatgg acaaatcgtc gatgctaacc tcgccgcgct
     6661 caatttctat ggttataacc atgaaacgat gtgccagaaa catacctagg aaataaatat
     6721 gcttgggcgt cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa
     6781 ccctttgaat tttgttcata aactggcgga tggatctact cgtcatgtgc agacctatgc
     6841 cggaccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga
     6901 gcaaaaacgg ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt
     6961 actgaatcgg cgacagtttt atcacattac ggaaccaggc caaatgcagc atctcgccat
     7021 cgctcaggat tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct
     7081 ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc gcccgccccc tcgaaagttg
     7141 cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc
     7201 aagaacccca ctggataccg cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa
     7261 agtgagtatt tcgggcttac cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg
     7321 aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa
     7381 tgatggacgc aaccgcgtgc tggcggcata agccgcggat gcgtctcgag atcaacgact
     7441 gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac
     7501 gtaacacgcc ccgctgccgc catgatgcgg tagcgcggtg caatatgcct gaacatcatc
     7561 aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgtg atttatcttc
     7621 ccgcccttta ccatgaataa tcagcacatt acgcaaacca tccgccaggg cttgttgaat
     7681 gaaactgaac accattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc
     7741 ctgttgcgga tatttaccac tgcgcagctt atccagcacc ccatgctaca acccttcctg
     7801 ccgaaactcc agcggctgac ttagcgggat gatgtcgaga aatccggtag tgaggaaatt
     7861 atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg
     7921 gacatctgta gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt
     7981 gtcgtcaagg ttcatgtaac ctcctatgca ccttcacacg tcgtgattca gacgggttaa
     8041 ttgttcatac ttactgcatc gttcccttca cagattgaaa caatactcta cttcatacct
     8101 gtgagtatct gcgcaggcgg ggcattttat actagcctgt tagcagtatc gaacagttta
     8161 gcccgagttt aattcagcac aggctccgat caggatgatt tattcatagc aggaaccaac
     8221 aaaaatgcga taaataacag accactgatt agcagaaagg taccgaaaat gccatagttt
     8281 gcgcctccct cagcatcaat tgactcaata atttggttga agccagtatt tccgatgtct
     8341 ggtcacttct actgcgatta aaaaataaag cacttctgga aatgaagtca ttcaccgtat
     8401 ggggaacaga aaccattttt tcacccattc tcgaaaaatg aaaattagtt cgattattga
     8461 gaatagagcc acacagggca ataccaatcg cgcccccaag attacgtgtt aaattaaata
     8521 caccagaggc taacttcagc cgttcttttg gaattccccc cagcgttaat gttacgatgg
     8581 gggccatcgc aaactgctgc gaaatccctc taattgcctg tggaaacagt aattcctgcc
     8641 atccccattc gtgggttata ggggtaaaaa gatacattga aaacacaaat ccgcccagcc
     8701 ccgccatcaa cagccatcgc agatttattt ttttacttaa ccagaagtaa aagggaacag
     8761 aaaataactg gaaaatacct gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc
     8821 cgcgaatctg ccccaaaaac acaggtatta aatatacagt agagaatatc ccaacccctc
     8881 cagaaaaaga aaaaaaacat cccagagtga aatttttatc cttaaaagca tgaagatcca
     8941 ttatcggatt ggatattgta agtgtacgcg cagcaaataa aataaaactc accaatgcca
     9001 gaactgatgt cagcaaaata gtattatcat ccagccatcc ccagcgcgct ccttcttcaa
     9061 gggtatattc cagacatccc agagtcatgg ccaacaggat aatactcgga taatcagcaa
     9121 ctttcagtaa agagagatca ggtttatcaa aattaaccag aaaaggaata ctcaaaacta
     9181 aatatatgcc aggtaatata ttgatataaa atagccatcg ccagtcgagg ttatcagtga
     9241 gccagccccc cagagttggc cctagcgtcg gtgacagtga ggccagcgca ctgactaccg
     9301 cagcagccaa ccccaactct ttaccctggt aataaataaa cgccatcgtg aaaactaaag
     9361 ggatcatgga tgcgccagca gctccctgta aagcacgaaa caaaatcatg atctgaatat
     9421 tccatgcaag tccacacgcg attccacacg cgatactcat taaagtaaat attcccgcgg
     9481 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
     9541 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
     9601 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
     9661 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
     9721 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
     9781 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
     9841 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
     9901 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
     9961 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    10021 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    10081 aatgccatca atcggcgca
//
LOCUS       DAZSNG010000131         9937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZSNG010000131 DAZSNG010000000
VERSION     DAZSNG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.132"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHW4Z_003514"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHW4Z_003514"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR8882992.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHW4Z_003515"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHW4Z_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR8882993.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHW4Z_003516"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHW4Z_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR8882994.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHW4Z_003517"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHW4Z_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR8882995.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHW4Z_003518"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHW4Z_003518"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR8882996.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHW4Z_003519"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHW4Z_003519"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR8882997.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHW4Z_003520"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHW4Z_003520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR8882998.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHW4Z_003521"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHW4Z_003521"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR8882999.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHW4Z_003522"
     CDS             complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHW4Z_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR8883000.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2587 c         2295 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aaccttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAZSNG010000132         9907 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZSNG010000132 DAZSNG010000000
VERSION     DAZSNG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.133"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..78
                     /locus_tag="ACHW4Z_003523"
     CDS             <1..78
                     /locus_tag="ACHW4Z_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883001.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="ACHW4Z_003524"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="ACHW4Z_003524"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR8883002.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="ACHW4Z_003525"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="ACHW4Z_003525"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8883003.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="ACHW4Z_003526"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="ACHW4Z_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR8883004.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /gene="ycaO"
                     /locus_tag="ACHW4Z_003527"
     CDS             complement(4693..6453)
                     /gene="ycaO"
                     /locus_tag="ACHW4Z_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR8883005.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6583..7209
                     /locus_tag="ACHW4Z_003528"
                     /pseudo
     CDS             6583..7209
                     /locus_tag="ACHW4Z_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7408..8496
                     /gene="serC"
                     /locus_tag="ACHW4Z_003529"
     CDS             7408..8496
                     /gene="serC"
                     /locus_tag="ACHW4Z_003529"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR8883006.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8567..9850
                     /gene="aroA"
                     /locus_tag="ACHW4Z_003530"
     CDS             8567..9850
                     /gene="aroA"
                     /locus_tag="ACHW4Z_003530"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR8883007.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2368 c         2483 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     7381 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     7441 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7501 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7561 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7621 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7681 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7741 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7801 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7861 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7921 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7981 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     8041 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     8101 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     8161 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     8221 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     8281 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     8341 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     8401 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     8461 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8521 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8581 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8641 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8701 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8761 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8821 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8881 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8941 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     9001 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     9061 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     9121 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     9181 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     9241 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     9301 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     9361 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     9421 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9481 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9541 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9601 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9661 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9721 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9781 tcccaaatgc acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     9841 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     9901 acattgt
//
LOCUS       DAZSNG010000133         9774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZSNG010000133 DAZSNG010000000
VERSION     DAZSNG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.134"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHW4Z_003531"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHW4Z_003531"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR8883008.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHW4Z_003532"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHW4Z_003532"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR8883009.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHW4Z_003533"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHW4Z_003533"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR8883010.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHW4Z_003534"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHW4Z_003534"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR8883011.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHW4Z_003535"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHW4Z_003535"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR8883012.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHW4Z_003536"
     CDS             complement(6031..6360)
                     /locus_tag="ACHW4Z_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR8883013.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHW4Z_003537"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHW4Z_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR8883014.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHW4Z_003538"
     CDS             7998..8171
                     /locus_tag="ACHW4Z_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR8883015.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHW4Z_003539"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHW4Z_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR8883016.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZSNG010000134         9763 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZSNG010000134 DAZSNG010000000
VERSION     DAZSNG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.135"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            383..1501
                     /locus_tag="ACHW4Z_003540"
     CDS             383..1501
                     /locus_tag="ACHW4Z_003540"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR8883017.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHW4Z_003541"
     CDS             1567..1815
                     /locus_tag="ACHW4Z_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR8883018.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHW4Z_003542"
     CDS             1880..2248
                     /locus_tag="ACHW4Z_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8883019.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHW4Z_003543"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHW4Z_003543"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR8883020.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFAAPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHW4Z_003544"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHW4Z_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR8883021.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHW4Z_003545"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHW4Z_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR8883022.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHW4Z_003546"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHW4Z_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR8883023.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHW4Z_003547"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHW4Z_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2388 a         2705 c         2369 g         2301 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccgc gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccagattaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg cag
//
LOCUS       DAZSNG010000135         9683 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZSNG010000135 DAZSNG010000000
VERSION     DAZSNG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.137"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(44..214)
                     /locus_tag="ACHW4Z_003548"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHW4Z_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHW4Z_003549"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHW4Z_003549"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR8883024.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHW4Z_003550"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHW4Z_003550"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8883025.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHW4Z_003551"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHW4Z_003551"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8883026.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHW4Z_003552"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHW4Z_003552"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8883027.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHW4Z_003553"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHW4Z_003553"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8883028.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="ACHW4Z_003554"
     CDS             5109..6242
                     /locus_tag="ACHW4Z_003554"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR8883029.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="ACHW4Z_003555"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="ACHW4Z_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8883030.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACHW4Z_003556"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACHW4Z_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8883031.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACHW4Z_003557"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACHW4Z_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8883032.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACHW4Z_003558"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACHW4Z_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8883033.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACHW4Z_003559"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACHW4Z_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8883034.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="ACHW4Z_003560"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="ACHW4Z_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR8883035.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2481 c         2466 g         2271 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tct
//
LOCUS       DAZSNG010000136         9610 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZSNG010000136 DAZSNG010000000
VERSION     DAZSNG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.138"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(22..342)
                     /gene="psiF"
                     /locus_tag="ACHW4Z_003561"
     CDS             complement(22..342)
                     /gene="psiF"
                     /locus_tag="ACHW4Z_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR8883036.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(461..1876)
                     /gene="phoA"
                     /locus_tag="ACHW4Z_003562"
     CDS             complement(461..1876)
                     /gene="phoA"
                     /locus_tag="ACHW4Z_003562"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR8883037.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1977..2237)
                     /gene="iraP"
                     /locus_tag="ACHW4Z_003563"
     CDS             complement(1977..2237)
                     /gene="iraP"
                     /locus_tag="ACHW4Z_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR8883038.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2419..2622)
                     /locus_tag="ACHW4Z_003564"
     CDS             complement(2419..2622)
                     /locus_tag="ACHW4Z_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883039.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2700..3794
                     /gene="ddlA"
                     /locus_tag="ACHW4Z_003565"
     CDS             2700..3794
                     /gene="ddlA"
                     /locus_tag="ACHW4Z_003565"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8883040.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3818..4030)
                     /locus_tag="ACHW4Z_003566"
     CDS             complement(3818..4030)
                     /locus_tag="ACHW4Z_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR8883041.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4290..4598
                     /locus_tag="ACHW4Z_003567"
     CDS             4290..4598
                     /locus_tag="ACHW4Z_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR8883042.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4657..5751)
                     /gene="yaiW"
                     /locus_tag="ACHW4Z_003568"
     CDS             complement(4657..5751)
                     /gene="yaiW"
                     /locus_tag="ACHW4Z_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR8883043.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5764..6984)
                     /gene="sbmA"
                     /locus_tag="ACHW4Z_003569"
     CDS             complement(5764..6984)
                     /gene="sbmA"
                     /locus_tag="ACHW4Z_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR8883044.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7336..8493
                     /gene="ampH"
                     /locus_tag="ACHW4Z_003570"
     CDS             7336..8493
                     /gene="ampH"
                     /locus_tag="ACHW4Z_003570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR8883045.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8494..8997)
                     /locus_tag="ACHW4Z_003571"
     CDS             complement(8494..8997)
                     /locus_tag="ACHW4Z_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883046.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9085..>9610)
                     /locus_tag="ACHW4Z_003572"
     CDS             complement(9085..>9610)
                     /locus_tag="ACHW4Z_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8883047.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2383 a         2452 c         2291 g         2484 t
ORIGIN      
        1 ccggggcggt tcaatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt
       61 acgatcatca cctttcagag cttgttgcgt tgcttgatta ttacattcac gcatcttttg
      121 ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact
      181 catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga
      241 ggtcatacgc tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa
      301 aaccagacca aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat
      361 gttttaacca tgagcgtttg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa
      421 aaaaccaccc ggcagcgaaa attcactgcc gggcgcagtt ttatttcagc cccagagcgg
      481 ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg
      541 ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggaat
      601 tcccgtaact catcaccatc actgcgccat ctttagtatt tagcgcctga gtgaggcccg
      661 gcgctttggt atccggcgca acaacctggc tggcgtgggc gtgatcagcg gtgactatga
      721 ccagcgtgtt accctccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat
      781 cgaccgtctc gccaatttgc ccacaagggt tcgcagcgtg atcctgttta tcgattgacg
      841 caccttcaac ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt
      901 cggtcatctg tgccagggtt ggtacactgt cattacgttg cggattaggc gtataggtga
      961 ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg ccctagccag cgcactggca
     1021 tattgccgtc agcaaacagg ccaagcaggg gtttttgctg attcgcttcc gtcaccgaat
     1081 tcagtgaggc agcatcgttc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg
     1141 tttttccctg ccattcacca gcggttgccg tttcagcaaa ggtttttgcg ccgccgccaa
     1201 gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt
     1261 ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgaag
     1321 tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac
     1381 cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa
     1441 tatcgacgcc cagcgcgccg ttataggttt tgacatcggt tgaccaggcg gttgctgatg
     1501 cagccgagtc ggtgacgtag tccggtttgc cagttttttt attcagcgca tagtgagtgt
     1561 attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat
     1621 aattacgtgc ggcagtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat
     1681 tttttgcagg tttatcgcta agagaatcac gcagagcggc ggtctgatca cccgttaagc
     1741 ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca
     1801 tttctggtgt ccgggctttt gtcacagggg taaacagtaa cggtaagagt gccagtgcaa
     1861 tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag
     1921 cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac
     1981 tgacaaggat gcttcaataa cttctttacg taatcgcgca gcagctctgt atcgtcgtca
     2041 ggaatgctgg catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaaccgc
     2101 tgttggtcat tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct
     2161 acctgcgcac acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca
     2221 gcaatgagat ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg
     2281 tttcacaact tcatagtgtc tggaagtatt atttttatga caaaggaaat actttgcgca
     2341 aatatttttt gtttgattgc cagcgaaacg tttctcttat ttaaataatt acccacacaa
     2401 aatataggct tttaattatc aacctttatt ataaataaaa gctatgaaat atattctctt
     2461 cgttaactta taattaagag aaaaaaactc accgcattgc catttaagcc aggaataaat
     2521 tagcttcccg ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt
     2581 caccagtacg ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga
     2641 atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga
     2701 tggaaaaact gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc
     2761 tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc
     2821 tgggcattga taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg
     2881 cagacgatcc tgcccatatt gcgttgcgcc cttcggcgac cagccttgcg caggtgccag
     2941 gtaaacatga gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg
     3001 tcattttccc gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc
     3061 gggtcgccaa tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggata
     3121 aagatgtcac caaacgtctg ctacgcgatg ccgggctgaa cattgcgcca tttattaccc
     3181 tgacgcgcgc taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac
     3241 cactgtttgt aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg
     3301 aagaacagta cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg
     3361 agcaagggat caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag
     3421 ccagcacctg tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca
     3481 ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat tgcaccagag atcaacgata
     3541 agatccgggc gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg
     3601 tagacgtgtt tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg
     3661 gcttcaccaa catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg
     3721 atctgatcac acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga
     3781 aaaccacaat gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt
     3841 catcgcgacg acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac
     3901 cgacggcata ggtctgccaa ccttttgctt caaatcccag tacccccacc acgccattaa
     3961 gaatgaatat aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag
     4021 gcagattcat ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg
     4081 tctgtggtgt agacaacatt ttcgtaatta ttaataaatt taacttaagg ttgccatttt
     4141 gtgactcaca acgaagattc acgcttcggg aatattccca acatcaattt accatgattc
     4201 tgatattgac aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga
     4261 agagaagcag atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt
     4321 ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg
     4381 tgctgttttg cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg
     4441 tctatgagcg tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag
     4501 gtgtcggcac cattttcggg ctgatcccgt ttttagcagg ctgcctcatt tttgcagtgg
     4561 tggcgctatg gctgcactgg cgacatcgcc gccagtaaaa tttacgttgc cagatgcggc
     4621 ctgaaacgcc ttatctggcc tgacattcct gtttgatcac tgtttcatac aacgcgcccg
     4681 ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct
     4741 ttccagttga atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc
     4801 aagttgataa actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc
     4861 taactgacgg cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc
     4921 cagttcggtt ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag
     4981 cttcacgccg ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca
     5041 gccagcgtta aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag
     5101 taaatggtaa gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt
     5161 accgtccatt ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg
     5221 catcggccca ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac
     5281 catgctgatc agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa
     5341 ccgttcgcta taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa
     5401 agcaggaatg tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa
     5461 gcccggaacg gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga
     5521 gtagacgttt tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt
     5581 cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac
     5641 atctatcgct ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa
     5701 aaccagaacc gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc
     5761 tttttagctc aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa
     5821 gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag
     5881 gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag
     5941 cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac
     6001 ctgcaaatag agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata
     6061 gtttttccgt acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc
     6121 gtcttcacca taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc
     6181 cagccccggc agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac
     6241 gatagcggca atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg
     6301 cgcggagagc gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa
     6361 actgacgccc atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg
     6421 tgcggcacct tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc
     6481 cgtacgccag cggaacacgt agtgactgac aaagaagttg ttcagtacac tgatcaccac
     6541 cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac
     6601 tttatgcggc gaacttagcg cggtttgaat cagatcatag aacggcgcat accaggcgtt
     6661 gacggcgacc ccgacttcca ccaaaaacca agtgacgaag ataatcagcg cagtaccgag
     6721 tattgaccag tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc
     6781 tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc
     6841 gctaatcggg atctgcccgg aagcgccggt gatacgtgcc acccagtcac ccccaccggc
     6901 ttgccagaag ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg
     6961 ctttgggaaa aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact
     7021 gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga
     7081 cccatagcaa ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac
     7141 aaaccaaaga ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg
     7201 gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta
     7261 attcgccctt tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat
     7321 ctatggacac caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca
     7381 ttgacctccg tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc
     7441 gatcgttatg ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt
     7501 atcgacggta accagcgcgt ctttcgtagt tatggtgaaa cgcgacctgg taataacgtt
     7561 cgcccgcagc tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa
     7621 atgctggtga aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat
     7681 gctccgccag gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg
     7741 gcaacccata ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg
     7801 tttgtctggc caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc
     7861 gcgccaggca gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg
     7921 gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag agcagattac ccatccattg
     7981 gggatgaaag acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgct
     8041 gagcgcggtg ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat
     8101 tccacgcctg gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag
     8161 cgcagtaacc aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa
     8221 gtgattggca tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg
     8281 gccccgaaag agggtcgtcc gaggattatt cagaagacag gcggtggtgg cggtttcatt
     8341 acctatatgg cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg
     8401 ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc
     8461 gggaataaac cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat
     8521 agcgaccagt ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac
     8581 ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat
     8641 acgtgatcgc aattcttcat tccagtcaat cattgataat aatgttgcgc gggtttgttt
     8701 ggctggtaga tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt
     8761 tttcattaaa ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac
     8821 gttttcttct ctacgcagag agataactcc ttctaaaata acgaaagtat cttcgttatt
     8881 gacatcactg gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct
     8941 ggacaaacat ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg
     9001 cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc
     9061 agtatttctt cggagtgaag aatattacca agtatattta acacccacgt tcgcggacca
     9121 gtcttgatct acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt
     9181 ggtgaagctg aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc
     9241 gatggaatca ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt
     9301 gaagtacgga gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc
     9361 cagttcataa cgcatgctgt cgtaaggctg accgtcaact ttcatgtcgt tgctcagctg
     9421 gtagtcatca ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc
     9481 atcacccagt ttgaagtcgt aaccggtttt caaaccgaag ccccaagcgt cggagttggt
     9541 gctaccgtca acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga
     9601 gtagctcaag
//
LOCUS       DAZSNG010000137         9537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZSNG010000137 DAZSNG010000000
VERSION     DAZSNG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.139"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHW4Z_003573"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHW4Z_003573"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR8883048.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHW4Z_003574"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHW4Z_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR8883049.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHW4Z_003575"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHW4Z_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHW4Z_003576"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHW4Z_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR8883050.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHW4Z_003577"
     tRNA            4965..5038
                     /locus_tag="ACHW4Z_003577"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHW4Z_003578"
     CDS             5173..6783
                     /locus_tag="ACHW4Z_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8883051.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHW4Z_003579"
     CDS             6815..7234
                     /locus_tag="ACHW4Z_003579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883052.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHW4Z_003580"
     CDS             8353..9099
                     /locus_tag="ACHW4Z_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8883053.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHW4Z_003581"
     CDS             9397..>9537
                     /locus_tag="ACHW4Z_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883054.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZSNG010000138         9493 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZSNG010000138 DAZSNG010000000
VERSION     DAZSNG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.140"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..190)
                     /locus_tag="ACHW4Z_003582"
     CDS             complement(<1..190)
                     /locus_tag="ACHW4Z_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8883055.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHW4Z_003583"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHW4Z_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR8883056.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHW4Z_003584"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHW4Z_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR8883057.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHW4Z_003585"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHW4Z_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR8883058.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHW4Z_003586"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHW4Z_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHW4Z_003587"
     CDS             complement(3262..3381)
                     /locus_tag="ACHW4Z_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR8883059.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHW4Z_003588"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHW4Z_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR8883060.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHW4Z_003589"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHW4Z_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR8883061.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHW4Z_003590"
     CDS             3803..4147
                     /locus_tag="ACHW4Z_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR8883062.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHW4Z_003591"
     CDS             4151..4486
                     /locus_tag="ACHW4Z_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR8883063.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHW4Z_003592"
     CDS             complement(4543..6864)
                     /locus_tag="ACHW4Z_003592"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8883064.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHW4Z_003593"
     CDS             complement(6981..7190)
                     /locus_tag="ACHW4Z_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883065.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACHW4Z_003594"
                     /pseudo
     CDS             complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACHW4Z_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(7941..9464)
                     /locus_tag="ACHW4Z_003595"
     CDS             complement(7941..9464)
                     /locus_tag="ACHW4Z_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8883066.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2206 c         2079 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAZSNG010000139         9443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZSNG010000139 DAZSNG010000000
VERSION     DAZSNG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.141"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1154
                     /locus_tag="ACHW4Z_003596"
     CDS             <1..1154
                     /locus_tag="ACHW4Z_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883067.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHW4Z_003597"
     CDS             1833..2051
                     /locus_tag="ACHW4Z_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR8883068.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHW4Z_003598"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHW4Z_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR8883069.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHW4Z_003599"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHW4Z_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR8883070.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHW4Z_003600"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHW4Z_003600"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8883071.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHW4Z_003601"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHW4Z_003601"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR8883072.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHW4Z_003602"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHW4Z_003602"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8883073.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHW4Z_003603"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHW4Z_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR8883074.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2448 g         2369 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc gtg
//
LOCUS       DAZSNG010000140         9431 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZSNG010000140 DAZSNG010000000
VERSION     DAZSNG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.142"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHW4Z_003604"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHW4Z_003604"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8883075.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHW4Z_003605"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHW4Z_003605"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR8883076.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHW4Z_003606"
     CDS             3115..3399
                     /locus_tag="ACHW4Z_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8883077.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHW4Z_003607"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHW4Z_003607"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR8883078.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHW4Z_003608"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHW4Z_003608"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR8883079.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHW4Z_003609"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHW4Z_003609"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8883080.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHW4Z_003610"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHW4Z_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR8883081.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2258 a         2488 c         2390 g         2295 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc g
//
LOCUS       DAZSNG010000141         9412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZSNG010000141 DAZSNG010000000
VERSION     DAZSNG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.143"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="ACHW4Z_003611"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="ACHW4Z_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8883082.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="ACHW4Z_003612"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="ACHW4Z_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8883083.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="ACHW4Z_003613"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="ACHW4Z_003613"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR8883084.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="ACHW4Z_003614"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="ACHW4Z_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR8883085.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="ACHW4Z_003615"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="ACHW4Z_003615"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR8883086.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="ACHW4Z_003616"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="ACHW4Z_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR8883087.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="ACHW4Z_003617"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="ACHW4Z_003617"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR8883088.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZSNG010000142         9203 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZSNG010000142 DAZSNG010000000
VERSION     DAZSNG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.144"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHW4Z_003618"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHW4Z_003618"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR8883089.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHW4Z_003619"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHW4Z_003619"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR8883090.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHW4Z_003620"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHW4Z_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR8883091.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHW4Z_003621"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHW4Z_003621"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR8883092.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHW4Z_003622"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHW4Z_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR8883093.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHW4Z_003623"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHW4Z_003623"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR8883094.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHW4Z_003624"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHW4Z_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR8883095.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..>9203)
                     /locus_tag="ACHW4Z_003625"
     CDS             complement(8064..>9203)
                     /locus_tag="ACHW4Z_003625"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR8883096.1"
                     /translation="VDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKN
                     PGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVI
                     VVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANA
                     DIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLA
                     AAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPLA
                     DLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSIS
                     SDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         2544 a         2146 c         2034 g         2479 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aac
//
LOCUS       DAZSNG010000143         9195 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZSNG010000143 DAZSNG010000000
VERSION     DAZSNG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.145"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..856)
                     /locus_tag="ACHW4Z_003626"
     CDS             complement(<1..856)
                     /locus_tag="ACHW4Z_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8883097.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHW4Z_003627"
     CDS             964..1242
                     /locus_tag="ACHW4Z_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883098.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHW4Z_003628"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHW4Z_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR8883099.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHW4Z_003629"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHW4Z_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR8883100.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHW4Z_003630"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHW4Z_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR8883101.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHW4Z_003631"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHW4Z_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR8883102.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHW4Z_003632"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHW4Z_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHW4Z_003633"
     CDS             complement(4922..5452)
                     /locus_tag="ACHW4Z_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR8883103.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHW4Z_003634"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHW4Z_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR8883104.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="ACHW4Z_003635"
     CDS             complement(6421..7644)
                     /locus_tag="ACHW4Z_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883105.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9195)
                     /locus_tag="ACHW4Z_003636"
     CDS             complement(8225..>9195)
                     /locus_tag="ACHW4Z_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8883106.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2682 a         1827 c         1925 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgatcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc tgcgg
//
LOCUS       DAZSNG010000144         9141 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZSNG010000144 DAZSNG010000000
VERSION     DAZSNG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.146"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHW4Z_003637"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHW4Z_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR8883107.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHW4Z_003638"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHW4Z_003638"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR8883108.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHW4Z_003639"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHW4Z_003639"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR8883109.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHW4Z_003640"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHW4Z_003640"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR8883110.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="ACHW4Z_003641"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="ACHW4Z_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR8883111.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /gene="smg"
                     /locus_tag="ACHW4Z_003642"
     CDS             5545..6018
                     /gene="smg"
                     /locus_tag="ACHW4Z_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR8883112.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="ACHW4Z_003643"
     CDS             6047..6589
                     /locus_tag="ACHW4Z_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR8883113.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="ACHW4Z_003644"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="ACHW4Z_003644"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR8883114.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="ACHW4Z_003645"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="ACHW4Z_003645"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR8883115.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="ACHW4Z_003646"
     CDS             7986..8243
                     /locus_tag="ACHW4Z_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR8883116.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="ACHW4Z_003647"
     CDS             complement(8219..8773)
                     /locus_tag="ACHW4Z_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR8883117.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2295 a         2218 c         2278 g         2350 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgatata gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaga agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
     9061 tacgccacct cgcgacagtg cgctaaagcg cgtcgcaact gctctttaac aatttatcag
     9121 acaatctgtg tgggcactcg a
//
LOCUS       DAZSNG010000145         9070 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZSNG010000145 DAZSNG010000000
VERSION     DAZSNG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.147"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..923
                     /locus_tag="ACHW4Z_003648"
     CDS             <1..923
                     /locus_tag="ACHW4Z_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8883118.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHW4Z_003649"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHW4Z_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR8883119.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHW4Z_003650"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHW4Z_003650"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR8883120.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHW4Z_003651"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHW4Z_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR8883121.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHW4Z_003652"
     CDS             4446..4805
                     /locus_tag="ACHW4Z_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR8883122.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHW4Z_003653"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHW4Z_003653"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR8883123.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHW4Z_003654"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHW4Z_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR8883124.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHW4Z_003655"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHW4Z_003655"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8883125.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2066 a         2196 c         2448 g         2360 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg
//
LOCUS       DAZSNG010000146         8778 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZSNG010000146 DAZSNG010000000
VERSION     DAZSNG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.149"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..390
                     /locus_tag="ACHW4Z_003656"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHW4Z_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..2289
                     /locus_tag="ACHW4Z_003657"
                     /pseudo
     CDS             362..2289
                     /locus_tag="ACHW4Z_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2273..2476
                     /locus_tag="ACHW4Z_003658"
     CDS             2273..2476
                     /locus_tag="ACHW4Z_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR8883126.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2473..4066
                     /locus_tag="ACHW4Z_003659"
                     /pseudo
     CDS             2473..4066
                     /locus_tag="ACHW4Z_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4056..5561
                     /locus_tag="ACHW4Z_003660"
     CDS             4056..5561
                     /locus_tag="ACHW4Z_003660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR8883127.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5598..5945
                     /locus_tag="ACHW4Z_003661"
     CDS             5598..5945
                     /locus_tag="ACHW4Z_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR8883128.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            6003..7031
                     /locus_tag="ACHW4Z_003662"
     CDS             6003..7031
                     /locus_tag="ACHW4Z_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR8883129.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHW4Z_003663"
     CDS             7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHW4Z_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8883130.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7481..7858
                     /locus_tag="ACHW4Z_003664"
     CDS             7481..7858
                     /locus_tag="ACHW4Z_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8883131.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7845..8429
                     /locus_tag="ACHW4Z_003665"
     CDS             7845..8429
                     /locus_tag="ACHW4Z_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8883132.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8426..>8778
                     /locus_tag="ACHW4Z_003666"
     CDS             8426..>8778
                     /locus_tag="ACHW4Z_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR8883133.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
BASE COUNT         2050 a         2070 c         2691 g         1967 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaggt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg caaggatgca
     2221 actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt gaatggttac
     2281 agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt
     2341 tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc tgaatgagct
     2401 taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc gccgaccgct
     2461 gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt
     2521 gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc agagtggtta
     2581 ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg gaatgcccgg
     2641 gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct gcataaggat
     2701 catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg gctggggatg
     2761 cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc ggaatacgca
     2821 gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga atttatccgt
     2881 gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt ctgggatacg
     2941 gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg ggtggacacg
     3001 ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga tcggtatggg
     3061 cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc cggacgctgg
     3121 gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat ttttgagccg
     3181 gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga gcggatgaag
     3241 atgctggact ccctgcaggc cacacagctt cagtcggcca ttgtgaaggc gatgtatgca
     3301 gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc gggggcaccg
     3361 caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg ctggtatgac
     3421 acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg gcgatgcgct
     3481 gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac aggcgctgct
     3541 gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa
     3601 ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt ttatggggcg
     3661 gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc tggaagaggc
     3721 gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc aggcgcgttc
     3781 agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg ggctcaagga
     3841 ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga aagaactggc
     3901 actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat ccgcagagcg
     3961 cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc agcagatagc
     4021 ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt tcacatatta
     4081 tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt
     4141 gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag caggtacagc
     4201 ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc aaacgacctg
     4261 cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc acgctggtac
     4321 accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc attgtcgcct
     4381 gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac attgacagtt
     4441 ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt ctccggcagc
     4501 tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg ttactggcat
     4561 cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt ggggtgatga
     4621 tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc acgctgattt
     4681 acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca aaggttcgcc
     4741 aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa gtggcgatgt
     4801 ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt gaaggtcagt
     4861 ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc atcagcgtga
     4921 tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta actgcaacgg
     4981 aggctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag gaagcgaaag
     5041 gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt gagcaggccc
     5101 gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag agtgaagccg
     5161 atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca acgctggcgg
     5221 cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca ccgcaggcga
     5281 atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa ggggcagagg
     5341 cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc cgggctgtac
     5401 tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc atgtttgaac
     5461 atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag acgtcagcag
     5521 acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa
     5581 tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg gaagtacgta
     5641 tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt tcctcggcaa
     5701 caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg gtctgggacg
     5761 gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt acagagacgg
     5821 tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg cctgagagag
     5881 tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac gcagcgctgc
     5941 cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa aggaactgat
     6001 ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa gttaaatttc
     6061 gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc gaagaggtaa
     6121 tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg gttgttgaag
     6181 gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc tacgttaagc
     6241 cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa gatccggccc
     6301 agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc aaacaggaag
     6361 agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg tatggcaaat
     6421 acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg tcggtgggaa
     6481 ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat acctttgatc
     6541 cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat attgccatta
     6601 tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa aaactggata
     6661 cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg gcggtggtgt
     6721 ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc tatgtggcag
     6781 aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga aatacggccg
     6841 cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct gaaggcgtgg
     6901 tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg cgtgaattca
     6961 ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc
     7021 aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa tcaggagcgg
     7081 gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag aggctggggc
     7141 gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg gaactggagg
     7201 aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca tcgaaagatg
     7261 atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt accggagacg
     7321 aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt aatggttcag
     7381 tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct gccagcatgg
     7441 cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt tcgataatct
     7501 gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg ggatcagcgc
     7561 cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg atgatcccga
     7621 aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt gcctgtttgt
     7681 gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg gggatgattc
     7741 tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc ggttaaggcg
     7801 gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag ggacttgaga
     7861 atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc agcgcatggg
     7921 cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt gcgcagaata
     7981 ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg gagcgtgtca
     8041 ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt aaccggggta
     8101 acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc cggcatggca
     8161 gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc cgggatgcgt
     8221 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac ggcaaaaatc
     8281 gttaccccat tgatgtggtg aaaatcccga tgtccggacc gctgacacag gcatttgaag
     8341 atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat gccctgaaac
     8401 aacaactgag gttacatctg agtaaatgaa ccgacacaca caaatccgtc agagcgtgct
     8461 ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc ttccggcatt
     8521 tattgatgcg caggaactgc ccgccgtggc ggtgtggctg agtgatgcgc agtacaccgg
     8581 aaaaatgacg gatgaggacg actggcaggc tgttctgcat attgccgttt tcgtccgtgc
     8641 acaggcgccg gattctgagc tggatacgtg gatggagaac accatatttc cggccatgaa
     8701 ggatattccg gtgctttccg gactcatcga caccatgatc ccactcggtt ttaactatca
     8761 acgtgataat gagatggc
//
LOCUS       DAZSNG010000147         8767 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZSNG010000147 DAZSNG010000000
VERSION     DAZSNG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.150"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..438
                     /locus_tag="ACHW4Z_003667"
     CDS             <1..438
                     /locus_tag="ACHW4Z_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR8883134.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="ACHW4Z_003668"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="ACHW4Z_003668"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR8883135.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="ACHW4Z_003669"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="ACHW4Z_003669"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR8883136.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="ACHW4Z_003670"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="ACHW4Z_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR8883137.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="ACHW4Z_003671"
     CDS             complement(4440..5147)
                     /locus_tag="ACHW4Z_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR8883138.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="ACHW4Z_003672"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="ACHW4Z_003672"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR8883139.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="ACHW4Z_003673"
     CDS             complement(6005..6208)
                     /locus_tag="ACHW4Z_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8883140.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6367..7875
                     /locus_tag="ACHW4Z_003674"
                     /pseudo
     CDS             6367..7875
                     /locus_tag="ACHW4Z_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8767
                     /locus_tag="ACHW4Z_003675"
     CDS             7872..>8767
                     /locus_tag="ACHW4Z_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8883141.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2036 a         2389 c         2251 g         2091 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacctg
     8761 aacaatt
//
LOCUS       DAZSNG010000148         8685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZSNG010000148 DAZSNG010000000
VERSION     DAZSNG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.151"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(42..134)
                     /locus_tag="ACHW4Z_003676"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACHW4Z_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHW4Z_003677"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHW4Z_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR8883142.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3223)
                     /gene="hemX"
                     /locus_tag="ACHW4Z_003678"
     CDS             complement(2012..3223)
                     /gene="hemX"
                     /locus_tag="ACHW4Z_003678"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR8883143.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3245..3985)
                     /gene="hemD"
                     /locus_tag="ACHW4Z_003679"
     CDS             complement(3245..3985)
                     /gene="hemD"
                     /locus_tag="ACHW4Z_003679"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR8883144.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3982..4923)
                     /gene="hemC"
                     /locus_tag="ACHW4Z_003680"
     CDS             complement(3982..4923)
                     /gene="hemC"
                     /locus_tag="ACHW4Z_003680"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR8883145.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5310..7856
                     /gene="cyaA"
                     /locus_tag="ACHW4Z_003681"
     CDS             5310..7856
                     /gene="cyaA"
                     /locus_tag="ACHW4Z_003681"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR8883146.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7896..8216)
                     /gene="cyaY"
                     /locus_tag="ACHW4Z_003682"
     CDS             complement(7896..8216)
                     /gene="cyaY"
                     /locus_tag="ACHW4Z_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR8883147.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8264..>8685)
                     /locus_tag="ACHW4Z_003683"
     CDS             complement(8264..>8685)
                     /locus_tag="ACHW4Z_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8883148.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1976 a         2241 c         2238 g         2230 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag
     2101 cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg cacgcgagtc tgcatcagtt
     2161 tttccagcat cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc
     2221 ttaactggtc cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag
     2281 cacgtaccca ggtggagacg ttctccagcg cctggcgata agtctcttcc tgatgacgcg
     2341 gtacagcttg tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct
     2401 gatttggcgc taacagcggt acggcggtgt catcacgacg gcgaatcgta atgaagttgt
     2461 ccataaagtt ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa
     2521 gctcttcacc gtctgaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt
     2581 tatctacctg atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg
     2641 atactgcaga aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg
     2701 ggtcattcat atccgccagg ctggcgtctg cacttttcag caacgctgca gaggtcgtga
     2761 cgtcctgatc gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca
     2821 gcagccaggt tttagcatcg ctgccggaaa tggtggcgat cttttgttgg acttcatcca
     2881 actgttttgc cagcgtttct tgctgacgat tcgcctgctt aagctgcgca gcttgttgct
     2941 taataatgcc ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt
     3001 ggttagccag ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat
     3061 acaaaccgac acccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcag
     3121 tattgttctt actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc
     3181 tggtctcttc aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg
     3241 agagttattg taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt
     3301 cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac
     3361 agtgtagtaa ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca
     3421 tttcaccgct ggtaacaacg accatcgtca cctcgcggga ttgccagcgc atcgcttctt
     3481 ctgcaccatc gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac
     3541 cgcgcaccgt cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg
     3601 cacgtttgcc cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc
     3661 gatcctgtgg gtagagaatc ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc
     3721 gtccaatggc gaaataagta ggtagttggg gccatttacg atcttgctga tgcagctgtg
     3781 attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg
     3841 ctgccagttg atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa
     3901 aatgccaggc cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag
     3961 acgggcgggt gaccaggata ctcatgccgg ggcctctccg ttatagactt cagcgaggat
     4021 ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatc
     4081 ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag
     4141 cgcacatagc cagatttcgc catcaataag ctcggcgtag ctaccaattg gcacctgaca
     4201 tccgccttcg agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg
     4261 gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca tcaaggcggc attcaatacc
     4321 gactgcacct tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg
     4381 ggactccaga cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc
     4441 gttatctagt ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc
     4501 cgggcggcgt tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc
     4561 tgctggtaac gcatccagac tgtcatagtt attggacaca aaggcatcgc gaggatcttc
     4621 acgctcgcaa atagtgacca gccccagacc ttgcgggaat tcaaccggca catctttcat
     4681 tgaatgtacg gcgatatcag cgcgattctc caggagcgcg acttccagct cttttacaaa
     4741 taagcctttt ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac
     4801 catcggtacc agttcaacga ccaggcccgg atggctcgcc atcaacttgt ctttgacata
     4861 gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa
     4921 catgcttgtt accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt
     4981 cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat
     5041 cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac
     5101 ttgtatgtag cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt
     5161 agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct
     5221 ttgacatacg aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc
     5281 atgacgggta gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga
     5341 gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat
     5401 tccaacaggt ctacagtcta ctgccgacat tgttgcacta tcaccatccg cgaatgccgg
     5461 gttaccttga tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac
     5521 gccactacct gaacgagctt gaactgtatc gtggaatgtc agtacaggat ccaccgaaag
     5581 gtgagcttcc aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt
     5641 gttcctctga cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc
     5701 agttactaca acgtaaatgc agcctgctgg aaagctgggc cgcctcgctg ggtgtggaag
     5761 tcagcttctt cctgattgat gaaaaccgct tccgtcataa tgaaagcggc agcctggggg
     5821 gcgaagattg tggctccacc cagcatatac tgctgcttga cgaattttat cgtactgccg
     5881 tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt
     5941 acgacgacta tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg
     6001 atctcggtgg cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc
     6061 tctacaagag tatcgattcc ccatacaaag cggtgctgaa aacactgctg ctggaagcct
     6121 attcctggga atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg
     6181 acggcgagat tgtatcgttt ggcctcgatc catactgcat gatgctggag cgtgttactg
     6241 aatacctgac ggcgattgaa gatttcaccc gtctggattt agtacgtcgc tgcttctatt
     6301 taaaagtgtg cgaaaaactc agccgtgaac gcgcctgtgt aggctggcgt cgcgcagtgt
     6361 tgagccagtt agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc
     6421 gtgctaactg gaagattgat caggtgcgtg aggcgcacaa cgagctgctc gacgcgatga
     6481 tgcagagcta ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca
     6541 gtccgcagga tatcggcgta ctgacgcgta aactgtatgc cgcgtttgaa gcattgccag
     6601 gtaaagtgac gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct
     6661 ttatttatgt gccgccgggc cgggctaacc gttcaggttg gtatctgtat aaccgcgcgc
     6721 caaatattga gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaataaac
     6781 tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg cacccgtttg tatattaaag
     6841 gtaacggcat tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt
     6901 tcccgctgcg cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagattcgcc
     6961 atctggcgat tatcgttaac ctggaatatg acccgacagc tgcgttccgc aatcaggtgg
     7021 tgcattttga tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg
     7081 taggtagcgt tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca
     7141 acggcgagca atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg
     7201 ccgcaccgcc agatagcgtg gaagtcttct gttatagcca gcatctgcgc ggcttaatcc
     7261 gtactcgcgt gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc
     7321 aggaaaccgg gcgtttcaag gcgctgcgcg tttctggtca aacctggggc ttgttcttcg
     7381 aacgcctgaa tgtatcggta cagaagctgg agaacgccat cgagttttat ggcgctattt
     7441 cgcataacaa actgcacggc ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg
     7501 cggtggtgga cggctttgcc agcgaaggga tcatccagtt ctttttcgaa gaaacgcaag
     7561 acgagaatgg ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtgtatcacc
     7621 actgcgaagg cagcaaagag gagctggtac gtgacgtcag tcgcttctac tcgtcatcgc
     7681 acgaccgctt cacctacggc tcaagcttca tcaacttcaa cctgccgcag ttctatcaga
     7741 ttgtgaaggt tgatggtcgc gaacaggtga ttccgttccg aacaaaatcg attggcaacc
     7801 tgccgcccgc caatcaggat cacgatacgc cgctattaca gcagtatttt tcgtgatgag
     7861 cgtgccggaa agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc
     7921 gcctgttgcg tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa
     7981 atccattcat cgcctttcag gtcaaaatgg tagccgccct gtttggttgc cagccacacc
     8041 tggtgcagcg gctcctggcg gttaataatg attttgctgc cgttttcaaa ggtaatggtc
     8101 agtacgccgc cgttgatttc gcagtcgata tcgctgtcgc catccctgtc gtccagacgc
     8161 tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt
     8221 gtatcctgtt tttaagtgat ggcggcagta tagcggcatg gggtcagggc ttcaaagttt
     8281 gcacctctgc tgctgcgttc cggcatgatt catccgtcac cggaataatg atgtctctgt
     8341 tttgaacttc acgtacgttc tcctctctga cacgagtttt cgccgggcag ggggagggcg
     8401 taatccttac ctctgtatcg gtccatgtat aaacattgcg atcgtcataa gcgaagcgat
     8461 cgtttttagt tagcgaaccg ttaactggaa taatttgcaa gctctttgac cgtaattggc
     8521 ggataaagta agcatgctgt ttgccgctat taaatgagcg atcaatgacc aggtctggcg
     8581 tatccagcgg gattatctca agctggtctt caccaaatgt cgataatttt gttacataca
     8641 gccgatcttt atattgcgca atattgccag taatcagatg gaatt
//
LOCUS       DAZSNG010000149         8604 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZSNG010000149 DAZSNG010000000
VERSION     DAZSNG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.152"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..265
                     /gene="csgC"
                     /locus_tag="ACHW4Z_003684"
     CDS             <1..265
                     /gene="csgC"
                     /locus_tag="ACHW4Z_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR8883149.1"
                     /translation="LEHLCCSEVSISPLFRWPNSVCHFKGSSGQSQTKQEKTLSLPAN
                     QPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQWTPSSEKS"
     gene            386..697
                     /gene="ymdA"
                     /locus_tag="ACHW4Z_003685"
     CDS             386..697
                     /gene="ymdA"
                     /locus_tag="ACHW4Z_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR8883150.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            792..1325
                     /gene="ymdB"
                     /locus_tag="ACHW4Z_003686"
     CDS             792..1325
                     /gene="ymdB"
                     /locus_tag="ACHW4Z_003686"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR8883151.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1267..2748
                     /gene="clsC"
                     /locus_tag="ACHW4Z_003687"
     CDS             1267..2748
                     /gene="clsC"
                     /locus_tag="ACHW4Z_003687"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR8883152.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2756..3913)
                     /gene="mdoC"
                     /locus_tag="ACHW4Z_003688"
     CDS             complement(2756..3913)
                     /gene="mdoC"
                     /locus_tag="ACHW4Z_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR8883153.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4307..5842
                     /gene="mdoG"
                     /locus_tag="ACHW4Z_003689"
     CDS             4307..5842
                     /gene="mdoG"
                     /locus_tag="ACHW4Z_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR8883154.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5835..8378
                     /gene="mdoH"
                     /locus_tag="ACHW4Z_003690"
     CDS             5835..8378
                     /gene="mdoH"
                     /locus_tag="ACHW4Z_003690"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR8883155.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2167 a         2040 c         2241 g         2156 t
ORIGIN      
        1 attggagcac ctgtgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattc
       61 agtgtgccac ttcaaaggca gttcagggca aagtcagacg aagcaagaaa agaccctctc
      121 attgcctgct aatcaaccca ttgctttgac gaagttgagt ttaaatattt ccccggaaga
      181 tcgggtgaaa atagttgtta ctgtttctga tggacaggca cttcatttat cacaacaatg
      241 gacgccctct tcagaaaagt cttaatttgt tgaaatatcg agcataagat gaatctggag
      301 agaatggtct gctgcgaatc agccaacctg aaagtatgga taacacaacc ttcaaggatg
      361 actaatcatt gaggaaatag aataaatgtt cagacctttt ttagactctc ttatgctcgg
      421 cagtttgttt tttcctttta ttgccattgc aggaagcacc gcgcaagggg gcgtgatcca
      481 tttttatggc caaattgtgg aaccggcatg tgacgtcagc acccagtcat cacccgtaga
      541 aatgaactgc ccacagaatg gttctattcc gggcaaaacc tactccagca aagcgttaat
      601 gagcggcaat gtcaaaaacg cgcaaatagc ctcagtaaaa gtgcagtatc ttgataaaca
      661 gaaaaagctg gctgtaatga acatcgaata taactaagtt tctgaacaac tcacgttgct
      721 gagcagaaaa atgcgattta accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt
      781 aaggaggcgt tatgaaaacg cgtattcatg ttgtgcaggg tgatattacc aaactggccg
      841 ttgatgtgat tgtgaatgcg gctaatccgt cattaatggg aggcggcggc gtcgatgggg
      901 ccattcatcg cgcagcgggt ccggccctgc tggatgcctg tttaaaagtc aggcaacagc
      961 agggcgattg ccctacgggg catgccgtta ttacgcttgc aggcgatctt cccgctaaag
     1021 ccgtagtaca caccgtcggg ccagtctggc gtggtgggga acaaaacgaa gaccagcttt
     1081 tgcaggatgc ctatctgaat agcctacgac tggtggcggc aaacagctat acgtcagtgg
     1141 cttttcctgc aatcagtact ggggtttata gttaccctcg tgcggcagcg gctgaaatcg
     1201 cagttaaaac cgtttcagaa tttattaccc gtcacgcttt acccgaacag gtatactttg
     1261 tctgttatga tgaagaaaac gcccacctct acgaaagact ccttacccaa caaggagatg
     1321 aatgatttgc cccggctggc gagcgcggtg ctgccactgt gttcgcaaca tcccggtcag
     1381 tgtggtcttt tccctctgga gaaaagtctg gatgcgtttg ccgcccggta tcgtctggcc
     1441 gaaatggcag agcatacgct ggatgttcag tattacatct ggcaggacga tatgtcgggg
     1501 cggttactgt tttccgccct gttagccgca gcaaagcgtg gcgttcgcgt ccgtttgttg
     1561 ctggacgaca acaatacgcc tggacttgac gacattttgc gcttgcttga cagtcatcca
     1621 cgcattgaag tccggctttt taatcctttc tcgtttcgct tgctacgtcc gcttggatat
     1681 atcaccgact tttcccgtct caatcgccgg atgcacaata aaagtttcac tgtcgatggc
     1741 gtggttaccc tagtgggagg acgaaatatt ggtgatgcct attttggtgc aggggaagag
     1801 ccactttttt cggatttaga tgtcatggca ataggccccg tggtagagga tgttgccgat
     1861 gatttcgccc gctactggta ttgcaaatcg gtttcaccct tacagcaggt gctggatgtc
     1921 ccggaaggtg aaatggcgga tcgcatcgag ttacccgcct cctggcataa cgatgccatg
     1981 acgcatcgtt atttacgcaa aatggaatcc agtccattta taaatcatct ggttgatgga
     2041 acgttgccgc ttatctgggc gaagacacgt ttattaagtg atgatccggc gaaaggggag
     2101 ggcaaggcaa aacggcattc actgttaccg cagcgcctgt tcgatatcat gggctcaccc
     2161 agtgaacgca tcgatattat ctcttcctat tttgtaccga cacgcgcagg tgtggcgcaa
     2221 ctcttacgga tggtgagaaa aggggtaaag attgcgatcc taaccaattc tcttgccgct
     2281 aacgatgttg ctgtcgtcca tgccggatac gcgcgctggc gcaaaaaatt gctccgctat
     2341 ggcgtggaat tatatgaact caagccgacg cgtgaacaaa gcagtacgtt acacgatcgc
     2401 ggcataaccg gtaattccgg tgccagcctg catgctaaaa cttttagcat cgatggtaaa
     2461 acggtgttta tcggctcttt caatttcgat ccgcgttcaa cattgctcaa tactgaaatg
     2521 ggcttcgtga tagagagcga aacgctggca cagttaattg ataaacgctt tattcagagc
     2581 cagtacgatg cggcctggca gctccgtctg gacaggtggg gacggatcaa ctgggttgat
     2641 cgtcatgcaa agaaagaggt tattctcaaa aaagaacccg ccaccagttt ctggaagcgg
     2701 gttatggtca gactggcgtc gatattgccc gtggaatggt tattgtaaat gtggcttaac
     2761 gggctggtgc tttatcgttc tcacgcttaa caaccggttt accagagaac aaaaacttca
     2821 gtaacgggat gcgtaaatga atttcataca gaattatcgc aatccctact acgaatatca
     2881 ggccacagag aaaaccaagc cagttggagg tgatgtgcgg tgtaatgtat gcgccgaaaa
     2941 acagcgttaa cgggtggtga accagataga taaacagcga tgcgttaaca aaataagtca
     3001 cccgcgctga ctggaagtta agcaaacggt ggccaaagga gaagaccaca ttcaccatcc
     3061 acaggccgag gaccatggtg atcaccgact cggtttcgta catccaggca tcgccactgc
     3121 catagcgctg gttgagtaaa taagcgacaa acgccaatgc tgcagcgagg gtacagccac
     3181 gagacggcgt ggtaaacaag gctttaagat gagggaaaat gaaagccagt gcgccgagga
     3241 taaagaacgg taaataaaat agcgtttgca tgacaataaa attgaacatg ccattactca
     3301 gaatgggtgg atacacaata aaaatcgttc ttcttattac cgcatagccg atgccgaggc
     3361 ataaaaaaat caccgatagt tttaccatcg agaatttttt attcgtttta tcagaatttt
     3421 ctaaattatt tctgatgcgc ttaaatatcc atacacacag cgtcgtcatg actactaaca
     3481 ccagtaaaaa ccacaggtgt gatattaatt cccaggccaa cgtattatat ttgtcataca
     3541 atgatagccc aggccaactt tccgctttcc ctttgacata ttgcagcata ataaattgcg
     3601 gtaatgtcag tagggggatg gctgttaaca tcgggatacc tacacgttcg acacgtactt
     3661 tccaccattt tttcaaggga tagcgtaaaa aaagcatgta ggaaaagtat ccggatataa
     3721 cgaaaaatac ctgcatgcgg aacgagtgga tgaagtcatt aaaaagggtt agccacaatg
     3781 acggttcggc gctattcaca tgccatgtat ggctcgaata gattaaagaa atatgaaaag
     3841 ggatccctaa caacatcagc caggcgcgga tggagtcgag gaaatattca cgttgcgcgg
     3901 gtactggatt catatatggt taactaatct cggatttttc gtcttatccc tgtcgggtta
     3961 tgcctttagg cttgttgcca tagcgacacc gacctgaccg cgccaggcgc aggcttcaag
     4021 gtttttatgc atagcatcat cgctaccact aaccagaatg gaagcgtctg taagacggtt
     4081 gataaataaa tttgctggca aaccctacac gaagtcgatg cttctgtctt taggagaagc
     4141 acggaaagtg aaaacggttg caatcaggtg cttaatccat gagccagcgt gctgaacgat
     4201 accgggattc agttgtcgga atggctggtt atccattaaa atagatcgga tcgatataag
     4261 cacacaaagg gggaagtgct tactaattat gaaacataaa ctacaaatga tgaaaatgcg
     4321 ttggttgagt gctgcagtaa tgttaaccct gtatacatct tcaagctggg ctttcagtat
     4381 tgatgatgtc gcaaagcaag ctcaatcctt agccgggaaa ggctatgagg cgcctaaaag
     4441 caacttgccc tccgttttcc gcgacatgaa atacgcggac tatcagcaga tccagtttaa
     4501 tcatgacaaa gcgtactgga acaatctgaa gaccccattc aaactcgagt tctaccatca
     4561 gggtatgtac ttcgataccc cggtcaaaat aaatgaagtg actgccaccg cagtcaaacg
     4621 aatcaaatac agcccggatt atttcacttt cggcgatgtt cagcatgaca aagacacggt
     4681 aaaagacctt ggttttgccg gttttaaagt gctttacccg atcaacagca aagataaaaa
     4741 cgatgaaatc gtcagcatgc tcggggccag ctatttccgc gtgattggtg caggtcaggt
     4801 ttatggcctt tctgcccgcg gcctagcaat tgataccgct ttgccatcgg gtgaagaatt
     4861 tccacgcttc aaagagttct ggatcgagcg tccaaaaccg actgataaac gtttaaccat
     4921 ttatgcattg cttgactcgc cgcgtgcgac aggtgcttac aaattcgtgg ttatgccagg
     4981 gcgtgacacg gttgtggatg tgcagtcgaa aatctatctg cgcgataaag tcggcaaact
     5041 gggggttgca ccgttaacca gtatgttcct gtttgggccg aaccaaccgt tgcctgcaaa
     5101 taactatcgt ccggagttgc acgactctaa cggtctgtct atccatgctg gtaatggcga
     5161 atggatctgg cgtccgttga ataacccgaa acatttagcg gtcagcagct tctccatgga
     5221 aaacccgcga ggctttggtc tgttgcaacg aggtcgtgat ttctcccgct ttgaagatct
     5281 cgatgatcgt tacgatctcc gtccaagcgc atgggtgact ccgaaagggg agtggggcaa
     5341 aggcagcgtt gagctggtgg aaattccaac caacgatgaa accaacgata acatcgtcgc
     5401 ttactggacg ccggatcagc tgccggagcc gggtaaagag atgaacttta aatacaccat
     5461 caccttcagc cgtgatgaag acaaactgca tgcgccagat aacgcatggg tgcaacaaac
     5521 gcgtcgttca acgggggatg tgaagcagtc gaacctgatt cgccagcctg acggtactat
     5581 cgcctttgtg gtcgatttta ccggcgcaga gatgaaaaaa ctgccagagg ataccccggt
     5641 cacagcgcaa accagcattg gtgataatgg tgagatagtt gaaagcacgg tgcgctataa
     5701 cccggttacc aaaggctggc gtctggtgat gcgtgtgaaa gtgaaagatg ccaagaaaat
     5761 cactgaaatg cgtgctgcgc tggtgaatgc cgatcagacg ttgagtgaaa cctggagcta
     5821 ccagttacct gccaatgaat aagacaactg agtacattga cgcaatgccc atcgccgcaa
     5881 gcgagaaagc agcattgccg aagactgata tccgcgccgt tcatcaggcg ctggatgccg
     5941 aacaccgcac ctgggcgcgg gaggatgatt ccccgcaagg ctcggtaaag gcgcgtctgg
     6001 aacaagcctg gccagattca cttgctgatg gacagttaat taaagacgac gaagggcgcg
     6061 atcagcttaa ggcgatgcca gaagcaaaac gctcctcgat gtttcccgac ccgtggcgta
     6121 ccaacccggt aggccgtttc tgggatcgcc tgcgtggacg cgatgtcacg ccgcgctatc
     6181 tggctcgttt gaccaaagaa gagcaggaga gcgagcaaaa gtggcgtacc gttggtacca
     6241 tccgccgtta cattctgttg atcctgacgc tcgcgcaaac tgttgtcgcg acctggtata
     6301 tgaagaccat tcttccttat cagggttggg cgctgattaa tcctatggat atggttggtc
     6361 aggatttgtg ggtttccttt atgcagcttc tgccttatat gctgcaaacc ggtatcctga
     6421 tcctttttgc ggtactgttc tgttgggtgt ccgccggatt ctggacggcg ttgatgggct
     6481 tcctgcaact gcttattggt cgcgataaat acagtatatc tgcgtcaaca gttggcgatg
     6541 aaccattaaa cccggagcat cgcacggcgt tgatcatgcc tatctgtaac gaagacgtga
     6601 accgtgtttt tgctggcttg cgtgcaacgt gggaatcagt aaaagccacc gggaatgcca
     6661 aacactttga tgtctacatt ctgagtgaca gttataaccc ggatatctgc gtcgcagagc
     6721 aaaaagcctg gatggagctt atcgctgaag tcggtggcga aggtcagatt ttctatcgcc
     6781 gccgccgtcg ccgcgtgaag cgtaaaagcg gtaatatcga tgacttctgc cgtcgctggg
     6841 gcagccagta cagctacatg gtggtgctgg atgctgactc ggtaatgacc ggtgattgtt
     6901 tatgcggcct ggtgcgcttg atggaagcca acccgaacgc cgggatcatt cagtcgtcgc
     6961 cgaaagcgtc cggtatggat acgctgtatg cgcgctgtca gcagttcgcg acccgcgtgt
     7021 atgggccact gtttacagcc ggtttgcact tctggcaact tggcgagtcg cactactggg
     7081 gacataacgc gattatccgc gtgaaaccat ttatcgagca ctgcgcactg gctccgctgc
     7141 cgggcgaagg ttcctttgcc ggttcaatcc tgtcacatga cttcgtagaa gcggcgttga
     7201 tgcgccgtgc aggttggggg gtctggattg cttacgatct cccgggttct tatgaagaat
     7261 tgccgcctaa cttgcttgat gagctaaaac gtgaccgccg ctggtgccac ggtaacctga
     7321 tgaacttccg tctgttcctg gttaagggta tgcacccggt tcaccgtgcg gtgttcctga
     7381 cgggcgtgat gtcttatctc tccgctccgc tgtggtttat gttcctcgcg ctctctactg
     7441 cattgcaggt agtgcatgcg ttgaccgaac cgcaatactt cctgcaacca cggcagttgt
     7501 tcccggtgtg gccgcagtgg cgtcctgagc tggcgattgc actttttgct tcgaccatgg
     7561 tgctgttgtt cctgccgaag ttattgagca ttttgcttat ctggtgcaaa ggaacgaaag
     7621 aatacggcgg cttctggcgc gttacattat cgttgctgct ggaagtgctg ttttccgtgc
     7681 tgctggctcc ggtacgcatg ctgttccata cggtcttcgt tgtcagcgcg ttccttggct
     7741 gggaagtggt gtggaattca ccgcagcgtg atgatgactc cacttcatgg ggcgaagcgt
     7801 tcaaacgcca cggctcacag ctgctgttgg ggttagtgtg ggctgttggg atggcgtggc
     7861 tggatctgcg tttcctgttc tggctggcac cgattgtctt ctcgttgatc ctgtcaccgt
     7921 ttgtttcggt gatttccagc cgtgccaccg ttggtctgcg caccaaacgc tggaaactgt
     7981 tcctgatccc ggaagagtat tcaccgccgc aggtgctggt tgataccgat cggttccttg
     8041 agatgaatcg ccaacgctcc cttgacgatg gctttatgca cgcagtgttt aacccgtcat
     8101 ttaacgctct ggcaaccgca atggcgacag cgcgtcaccg cgccagtaag gtgctggaaa
     8161 tcgcccgtga ccgccacgtt gaacaggcgc ttaacgagac gccagagaag ctgaatcgcg
     8221 atcgtcgcct ggtgctgcta agcgatccgg tgacgatggc ccgtctgcat ttccgcgtct
     8281 ggaattcccc tgaaagatat tcttcatggg tgagttatta cgaagggata aagctcaatc
     8341 cactggcatt gcgtaaaccg gatgcggctt cgcaataaaa acgtagttgc ctgatgcgct
     8401 acgcttatca ggcctacatc gttcctgcaa tttattgatt ttgcaagatt ttgtaggtcg
     8461 gataaggcgt tcacgccaca tccggcaaaa acaacgagcc aataaaaata ccggcgttat
     8521 gccgtaatgc cagtcagtta agcaactgac tggctctttt tcggggctgt ggggtatttc
     8581 cagggcctct cctttaccac tctc
//
LOCUS       DAZSNG010000150         8486 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZSNG010000150 DAZSNG010000000
VERSION     DAZSNG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.153"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            45..398
                     /locus_tag="ACHW4Z_003691"
     CDS             45..398
                     /locus_tag="ACHW4Z_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR8883156.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHW4Z_003692"
     CDS             complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHW4Z_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR8883157.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHW4Z_003693"
     CDS             complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHW4Z_003693"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR8883158.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHW4Z_003694"
     CDS             complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHW4Z_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR8883159.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHW4Z_003695"
     CDS             complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHW4Z_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR8883160.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHW4Z_003696"
     CDS             complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHW4Z_003696"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR8883161.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHW4Z_003697"
     CDS             complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHW4Z_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8883162.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="ACHW4Z_003698"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="ACHW4Z_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR8883163.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /gene="hycB"
                     /locus_tag="ACHW4Z_003699"
     CDS             7002..7613
                     /gene="hycB"
                     /locus_tag="ACHW4Z_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR8883164.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7610..8486
                     /locus_tag="ACHW4Z_003700"
                     /pseudo
     CDS             7610..8486
                     /locus_tag="ACHW4Z_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1900 a         2304 c         2286 g         1996 t
ORIGIN      
        1 gattaagatg taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta
       61 tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc
      121 aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg
      181 ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc
      241 agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt
      301 ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac
      361 tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt
      421 ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac
      481 agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg
      541 ctgttggttt ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg
      601 acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc
      661 tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt
      721 acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg
      781 atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata
      841 tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag
      901 agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca
      961 attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc
     1021 tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg
     1081 aacagagagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgtt ggcaccggta
     1141 aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg
     1201 cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca
     1261 atcagctctt tacccgagcc agtttcaccg aggatcagca cggtactgtc actttgcgcc
     1321 accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca
     1381 aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt
     1441 tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt
     1501 tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc
     1561 gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt
     1621 tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc
     1681 tcgtagagat tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg
     1741 gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag
     1801 tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg
     1861 aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa
     1921 agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac
     1981 tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc
     2041 tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgctc gtcataacga
     2101 ataaattcac agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga
     2161 ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg
     2221 taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga
     2281 acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca
     2341 cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt
     2401 accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga
     2461 gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc
     2521 ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga
     2581 attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac
     2641 gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc
     2701 gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc
     2761 aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc ccagcaattc
     2821 gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca
     2881 accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg
     2941 cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca
     3001 gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc
     3061 gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc
     3121 ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc
     3181 cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc
     3241 ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag
     3301 gataaagcca caggagagat agcgcggaat agcgccactg cccgcaacgt cattcgcggt
     3361 gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta
     3421 actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg
     3481 tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt
     3541 gattaattgc tgcatcgcct ggctgccgct gccgtgggcg agttggatat tattcacgct
     3601 tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc
     3661 agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc
     3721 ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc
     3781 ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg
     3841 ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca
     3901 tcggcaatcg cctgttgcgc cagtaggtta ccggcatccg gtaccacccg acgatactga
     3961 ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt
     4021 agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa
     4081 ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa cgcgtcgata
     4141 ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag
     4201 aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc
     4261 ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc
     4321 gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt
     4381 gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag
     4441 atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg
     4501 cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg
     4561 ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt
     4621 tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc
     4681 tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg
     4741 cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt
     4801 cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc
     4861 gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc
     4921 cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta
     4981 tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc
     5041 ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc
     5101 aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc
     5161 ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc
     5221 tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc
     5281 gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg
     5341 tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg
     5401 aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct
     5461 gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga
     5521 aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag
     5581 acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc
     5641 cggtgtgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct
     5701 gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc
     5761 aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt
     5821 gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg
     5881 aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg
     5941 gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc
     6001 ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc
     6061 aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac
     6121 cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg
     6181 ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat
     6241 aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg
     6301 tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc ctggaa
//
LOCUS       DAZSNG010000151         8374 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZSNG010000151 DAZSNG010000000
VERSION     DAZSNG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.154"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACHW4Z_003701"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACHW4Z_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACHW4Z_003702"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACHW4Z_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR8883165.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACHW4Z_003703"
     CDS             complement(1288..2046)
                     /locus_tag="ACHW4Z_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR8883166.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="ACHW4Z_003704"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="ACHW4Z_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR8883167.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="ACHW4Z_003705"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="ACHW4Z_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR8883168.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="ACHW4Z_003706"
     CDS             3759..4619
                     /locus_tag="ACHW4Z_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR8883169.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="ACHW4Z_003707"
     CDS             complement(4660..5196)
                     /locus_tag="ACHW4Z_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR8883170.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="ACHW4Z_003708"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="ACHW4Z_003708"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR8883171.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="ACHW4Z_003709"
     CDS             6174..6764
                     /locus_tag="ACHW4Z_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8883172.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="ACHW4Z_003710"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="ACHW4Z_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..>8374)
                     /locus_tag="ACHW4Z_003711"
     CDS             complement(8291..>8374)
                     /locus_tag="ACHW4Z_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883173.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         2171 a         2004 c         2009 g         2190 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctgggcc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact cata
//
LOCUS       DAZSNG010000152         8051 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZSNG010000152 DAZSNG010000000
VERSION     DAZSNG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.155"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(363..438)
                     /locus_tag="ACHW4Z_003712"
     tRNA            complement(363..438)
                     /locus_tag="ACHW4Z_003712"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Asn,seq:gtt)
     gene            complement(539..2010)
                     /gene="yeeO"
                     /locus_tag="ACHW4Z_003713"
                     /pseudo
     CDS             complement(539..2010)
                     /gene="yeeO"
                     /locus_tag="ACHW4Z_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2171..2246
                     /locus_tag="ACHW4Z_003714"
     tRNA            2171..2246
                     /locus_tag="ACHW4Z_003714"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2204..2206,aa:Asn,seq:gtt)
     gene            complement(2284..3234)
                     /gene="cbl"
                     /locus_tag="ACHW4Z_003715"
     CDS             complement(2284..3234)
                     /gene="cbl"
                     /locus_tag="ACHW4Z_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR8883174.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3336..4253)
                     /gene="nac"
                     /locus_tag="ACHW4Z_003716"
     CDS             complement(3336..4253)
                     /gene="nac"
                     /locus_tag="ACHW4Z_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR8883175.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4581..4656
                     /locus_tag="ACHW4Z_003717"
     tRNA            4581..4656
                     /locus_tag="ACHW4Z_003717"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Asn,seq:gtt)
     gene            complement(4712..5647)
                     /gene="ldtA"
                     /locus_tag="ACHW4Z_003718"
     CDS             complement(4712..5647)
                     /gene="ldtA"
                     /locus_tag="ACHW4Z_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8883176.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5709..6788)
                     /gene="cobT"
                     /locus_tag="ACHW4Z_003719"
     CDS             complement(5709..6788)
                     /gene="cobT"
                     /locus_tag="ACHW4Z_003719"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR8883177.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6800..7543)
                     /gene="cobS"
                     /locus_tag="ACHW4Z_003720"
     CDS             complement(6800..7543)
                     /gene="cobS"
                     /locus_tag="ACHW4Z_003720"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR8883178.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7540..8049)
                     /gene="cobU"
                     /locus_tag="ACHW4Z_003721"
     CDS             complement(7540..8049)
                     /gene="cobU"
                     /locus_tag="ACHW4Z_003721"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR8883179.1"
                     /translation="MVVSAIASTPHSSQVLYIATSQILDDEMAARIEHHRQSRPEHWR
                     TVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINA
                     EIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLV
                     VSGIGVKIK"
BASE COUNT         2233 a         2150 c         1740 g         1928 t
ORIGIN      
        1 taaaaaatcc ccgtccccag agaaataata tttattaaaa tggcaggtct tctttttctg
       61 attaccgaag acaaagtggc aattacgtat agtttcccga taaagacgcg acagctacgt
      121 cccgcataag gcatttttct ctttatcttt gtacggtact tcatggaaca gaatttttga
      181 ccttgtgaat cgtgatatct gttggggagg ggcaatttgc tcactgaagc gtgagactcg
      241 attaagcgca cgaaacacag aaatcaaaaa acccggccac ttttttacaa agtggccggg
      301 taaaaataat ttttattttt taactgtcct gagtctcata gagatgtctc aaaactaaaa
      361 tttggctcct ctgactggac tcgaaccagt gacatacgga ttaacagtcc gccgttctac
      421 cgactgaact acagaggaat cgtgagaacg aggcgaatat tagcgatgcc cacccacaat
      481 gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt
      541 attccgacac aactggcttt ttttcacact tttgcggctc ggatcgaggg tatttccaca
      601 gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa
      661 acattcccat ccagacacca accacacccc agccaagcat gattccctgc acataaccga
      721 ctacaacccg gcaaccccac atgctcaaca tagaaaccca catggcgtaa cgggcatcac
      781 gagcaccctt aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag
      841 catttagcta aatcagaatc acaacgacgt gtttaacctg tggatcctgg gtgtaaaacg
      901 atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg
      961 acagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc
     1021 ttcggcctgt aatgatcgta gaagcagaac cgagcgcact tccgggtaag ttgataagag
     1081 ccgcaattga gaacgcgata aaatttccgg caataacgct ggtccccatc ccggcaacga
     1141 acatttgggt taataaccga ccactggtaa ataacactga ttcgacactc gctggaatac
     1201 caatccccat gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg
     1261 aaatccttag cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa
     1321 tataacgaga aatggttaaa cccaacccag ccccgaccag gagaaaaggc cgtaaatcaa
     1381 tatgccgcta ataataatat taagaatatt caggctaccg ttaatcagta gcggtatttt
     1441 cgtattccct gcaccacgaa gtgccccgct accaataaga gtgatggcag cggctggata
     1501 actgagtacg gtcagctcca gataagtcaa cgccagtgct ttaacttctg tcgtggcatc
     1561 acccgcgacg aaatcaataa tttgttcgcc aaaatgatga attagcgttg ccaacagtac
     1621 ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc
     1681 ccgcttaccg agactaaatg ctacaacgac agtagtacca agatcgatag ctgcaaaaaa
     1741 agccataatg accatattga agctgtccgc caaccccacg ccggccatcg catcttttcc
     1801 cagccaactg accagaaaag tgctcaggac ccccatcaac aggacacagg cgttctccat
     1861 gaagatagga acagcaagcg gggttatctc gcgctagaac aacactttgt agctcttgcg
     1921 tttagagtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc
     1981 cgaccttaat tgcagaaagt gaaaccacat ttcaaatgat gagggagaat cagcaaagct
     2041 gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata gaaacggcaa
     2101 acagttggga aatttaaaaa tcgggtttga caaaatattt ttcgccgtta agatgtgcct
     2161 caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     2221 tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat agcgggcttt
     2281 ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctggc gcttgatatc
     2341 ctcaactgac agtcctgcat tacaaagttc cagaaagcgc caaacatagt tacgctgaag
     2401 ttgtcctcgc ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag
     2461 gcggattaaa ttctcttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc
     2521 aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgtgcactta atacaatatc
     2581 tgccggcaaa cctttgcggg taaatgcgtc atcagtgcgt gagcgccccg taattccctg
     2641 tcggtaagta attaacggcc acttcgctat tgattccagc gtcaatggtg taatttgcgt
     2701 taagggatga tcgagtggaa caagcaaact atggtgccaa caaaaccatg ggaaggcgac
     2761 gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg
     2821 caacaacgtc gcaatttcct gtggcgaacc ctggattagc tcgagccgaa cctccgggaa
     2881 aagttcgcga aaagctttaa tgacctctgg caagctataa cgtgcctgag tatgcgtcgt
     2941 tgcaatagtg agaacaccag acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt
     3001 actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg gttcagtcat
     3061 ccccagcagt cgcttacctc gtcgaacaaa tatttcgatg ccaaattcat cctccagttc
     3121 ccgaatatga cggctaacgc ctgactgtga ggtaaaaagc atattcgcaa cctctgtcag
     3181 gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa
     3241 ctccgggcag ttcagatatc ccgttattgt taaagtctaa tgcccggcat aacaaataat
     3301 aaaaacccgc atcttattcc atcccgatat aacgcttagc tcaccaattg ccactgcctt
     3361 ttttccatca ctggagaact aatcactgac attaacaact ctttcactgc ctgtgcctgt
     3421 ggtgacaagt ttgctctggc gggtaaattt aaggacaaag agagactcat tgaaggagtg
     3481 gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcagc cgattcaggt
     3541 aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat agactcaatt
     3601 tcaccaatga cttttgccgt gagccgccgt agggaaaaag cctcatcaac acgaagtcta
     3661 acagcgctgt aatcactggg gagaaaaagg ttcatttgcg caatagcatt aacatcaacg
     3721 ttttgccccg ggcaatcttg agttcctacc aggaaaagat cttctttcag caaagcctga
     3781 ctggataccc cagccacagg ggaatgctca taaatcaccg ccatatcgag ttggtgattt
     3841 atcagttttt cgttaagcac tgcaccacta ttttcatgaa gatagataac gacctccggg
     3901 aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc ggttcctggc
     3961 gcaaagccaa tcgagacttg ccccgataat gactgaccaa cgttatgcac cgccagttgg
     4021 gcctgctcac actgacgtaa aatggcccgt gcatgggtat agagaatttt tccggcgtct
     4081 gttggcgtaa cgccccgttt tgtgcgaatc aaaagctgtt gatttaactc accttccagt
     4141 atggcaacct gttggctgag cgctggttgt gcgatatgca atacttcagc agcctgggtc
     4201 aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt catgttgcct
     4261 ccggttttta agaatcggcc caagtgccgc cattacttac aaccagtatg caagatgcct
     4321 gccagttcta ttttggcgtt gatgcgcaag ctaaccaact gtcaaataag agattatgat
     4381 agattcgtca ttggcccttt ttatcagcag tcgcattcac ctgccctata aaaggagggt
     4441 atgcaccacg atggttcatt acccaataag attgaaagct caccactttg ttgaaattgg
     4501 cagcaaacaa acaaaaaaaa tgcatttcac cctttgacat caccatgcac tgccattaat
     4561 atgcgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     4621 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caaaaaagcc tgctttcgag
     4681 caggcttttt actttctaat taccaacgct cttaaaacat ctgtcttgaa ccagaactaa
     4741 tttgcacagg cattcccgat cgatgttgca acgcagcatt tgcgcgattt acatcaactt
     4801 cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg caatggcact tttcggtcag
     4861 actcatattc tgcacgattg cgcgacagcg gctcatgaac ttccagccac tttgaaccat
     4921 ccggttccgt tgtgtatttc actggctgat caataatttg tacacgagtg cctacaggaa
     4981 cattatcaaa cagatatttg atatcgtcat tacgcagacg aatacagccc tgacttacac
     5041 ggagcccaat accaaaattg gcattggtac cgtggatggc atacaacctg ccaatataaa
     5101 tcgcgtacag ccccatgggg ttatcgggcc ccgcaggaac aaatgcgggc aaactctccc
     5161 ctcgtttcgc atattcgcgc cggatgttcg gcgttggcgt ccaggttggt gcttcttgtt
     5221 tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg ccgataccaa
     5281 taggaaagac ttccactgta ttactgtctg gcggatagta ataaagacgc atttcagcga
     5341 cgttaaccac aatcccttta cgaacagtgg cgggcaaaat cagttgctgc ggaatggtga
     5401 gttgtgagcc aggcttcggc aaaaaaacat cagcgcccgg attcgcttcc agcatattac
     5461 ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgagtattg tgatcaggaa
     5521 cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat ggataagtta
     5581 ccgccaggct attttggctg gcaaaaagca gagcaaatga gcaaagaata tttacacgac
     5641 gcatcatgtc cctttcctaa gtcgcgaata ctatccgata agtatagctt ttatcagact
     5701 tttcgttttt agctgttcaa atcagaagtc gtattccccg gtagaacaat attactggca
     5761 gcaagttcgc ccatgttgtt gtatatcgca caagcagctt cgatgatgga catcgccaga
     5821 gctgcaccac tcccctcacc taaacgcata tccatattga gataaggctc cagccccaaa
     5881 tgcgagagcg ctatacgcgc gcctttttct gccgacaagt gagaaggaat gagatacggt
     5941 ttgattgcag gagacatctg gcaggctgcg agcgcagcag cataagaaag aaatccatcc
     6001 agcaacacgg gtaaaccgca ggaagcagca cctaacatca ctccagctat tccgaccaaa
     6061 tcaaatccac ccacttttgc caggacatca acaccatcct gaggatttgg ttgattcaac
     6121 gtaatcgccc gacgcacaac atcaatttta ttagccagtt tatctgtcgg caggtttgcg
     6181 ccaatcccaa ccacttcttc aggatcccgg ccagtgattg tgctgactat tgccgctgcc
     6241 ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtgacacc gtttttcgcc
     6301 agctcccgcg tataacatat gacgtccaaa agcaactttt cagcctgacg gcgactcatt
     6361 gccggagctg aagcaatatt gccgctacct cgtgcgacac gcatgttgat aagcccgggg
     6421 ataggctcag cagtatcaat accaacatca attacgtgga cgttagcgcc cgcttgtgct
     6481 gccagcacac acacgccggt tgttccacgg gtcatatttt cagcctgtat ggctgtcact
     6541 tcttttggag aaatagcgac cccttcctcc cagacgccgt gatcggcaca cataaccagt
     6601 accgcttttt tgcccacatg cggtatgcca ttcacccccg gcattcctgc cagttgtatg
     6661 gcaagcgcct ccagctttcc taggctacca acaggtttga ataacccgtc aacatgccgt
     6721 tgtgcacgcg acatagcggc agaattgatg gctgggatcg tattcagtaa atcggcaatt
     6781 atttgcatgt cacgtttctt tatagcagtg ccagcaggaa gaccagttca ccaagctcta
     6841 tggctgctcc cagcgtatcg cccgtttgcc cgcccagcgt acgtttgaga agctgaccga
     6901 ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata cccagcaata
     6961 atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc ccatcaattt
     7021 tgccaatgaa cacattgcca agcccttctt cccgcgcata acgatgacga tacatcaata
     7081 atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg atcggttcgc
     7141 cacgcagagc caactcgctt aacaccagaa tctttgccag taccacaaaa atcaatgcca
     7201 gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga tcgcgactac
     7261 gtgcagaaaa tacgccatca caggtatcag cgaggccgtc cagatggaat tccccggtca
     7321 tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tgcgccacac catgcctgca
     7381 gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaatcaaa ggaaaagtaa
     7441 taataccgcg agaataatgt tcgaaatcca gtccctggga ccagcgacgc ggtacaggca
     7501 gacgcgtaat aaatgagagc atcgcccaaa ataatttgct catttgattt ttactccaat
     7561 acccgaaacc accagccata cttcatttgc cgcagcggcc aactgctgat ttacccgccc
     7621 ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca ttcccacttc
     7681 gttagtcact aatacaacct ttgcggggca acgttggcag gcagcaatca acgactgaat
     7741 ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt ctttatcgcc
     7801 gccataatca aacaacagat tagtcaccat tgtggtaacg cattccagca acacagcctc
     7861 attcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct ccactgtgcg
     7921 ccagtgctcc gggcggcttt gccgatgatg ttctatccgt gcagccatct catcatcaag
     7981 gatttgcgag gtagcgatat acagaacctg tgaagagtga ggtgtactgg caatagcgga
     8041 cactaccatt t
//
LOCUS       DAZSNG010000153         7881 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZSNG010000153 DAZSNG010000000
VERSION     DAZSNG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.156"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHW4Z_003722"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHW4Z_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHW4Z_003723"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHW4Z_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR8883180.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHW4Z_003724"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHW4Z_003724"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR8883181.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHW4Z_003725"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHW4Z_003725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR8883182.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHW4Z_003726"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHW4Z_003726"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR8883183.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHW4Z_003727"
     tRNA            7783..7858
                     /locus_tag="ACHW4Z_003727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1785 a         2041 c         2134 g         1921 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttaaaagt gctgtaaggc a
//
LOCUS       DAZSNG010000154         7859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZSNG010000154 DAZSNG010000000
VERSION     DAZSNG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.157"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..86)
                     /locus_tag="ACHW4Z_003728"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="ACHW4Z_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            84..408
                     /locus_tag="ACHW4Z_003729"
                     /pseudo
     CDS             84..408
                     /locus_tag="ACHW4Z_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="ACHW4Z_003730"
     CDS             complement(438..3350)
                     /locus_tag="ACHW4Z_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR8883184.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3833..4675
                     /locus_tag="ACHW4Z_003731"
     CDS             3833..4675
                     /locus_tag="ACHW4Z_003731"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8883185.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="ACHW4Z_003732"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="ACHW4Z_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR8883186.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="ACHW4Z_003733"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="ACHW4Z_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR8883187.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="ACHW4Z_003734"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="ACHW4Z_003734"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8883188.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2116 a         1759 c         1860 g         2124 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgttta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agtacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tgctaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag
     4381 cgggagggaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatat tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DAZSNG010000155         7830 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZSNG010000155 DAZSNG010000000
VERSION     DAZSNG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.158"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACHW4Z_003735"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACHW4Z_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR8883189.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="ACHW4Z_003736"
     CDS             1102..2562
                     /locus_tag="ACHW4Z_003736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8883190.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACHW4Z_003737"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACHW4Z_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR8883191.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACHW4Z_003738"
                     /gene_synonym="ydfH"
     CDS             complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACHW4Z_003738"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR8883192.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACHW4Z_003739"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACHW4Z_003739"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR8883193.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="ACHW4Z_003740"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="ACHW4Z_003740"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR8883194.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="ACHW4Z_003741"
     CDS             complement(6727..7245)
                     /locus_tag="ACHW4Z_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR8883195.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..7745
                     /gene="ydeI"
                     /locus_tag="ACHW4Z_003742"
                     /pseudo
     CDS             7521..7745
                     /gene="ydeI"
                     /locus_tag="ACHW4Z_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
BASE COUNT         1972 a         1828 c         1978 g         2052 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcttggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aaggagtgag gtagcctgag tttaacggac actccttcct gaaatagaat ggcatcagaa
     7801 ggagctaata atgagcagaa aaacccaacg
//
LOCUS       DAZSNG010000156         7723 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZSNG010000156 DAZSNG010000000
VERSION     DAZSNG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.159"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..832)
                     /locus_tag="ACHW4Z_003743"
     CDS             complement(<1..832)
                     /locus_tag="ACHW4Z_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8883196.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHW4Z_003744"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHW4Z_003744"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR8883197.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHW4Z_003745"
     CDS             complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHW4Z_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR8883198.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHW4Z_003746"
     CDS             complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHW4Z_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR8883199.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHW4Z_003747"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHW4Z_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR8883200.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHW4Z_003748"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHW4Z_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR8883201.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="ACHW4Z_003749"
     CDS             complement(5530..6513)
                     /locus_tag="ACHW4Z_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8883202.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="ACHW4Z_003750"
     CDS             complement(6559..7383)
                     /locus_tag="ACHW4Z_003750"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8883203.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHW4Z_003751"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHW4Z_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR8883204.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac tca
//
LOCUS       DAZSNG010000157         7552 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZSNG010000157 DAZSNG010000000
VERSION     DAZSNG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.160"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..694
                     /locus_tag="ACHW4Z_003752"
     CDS             <1..694
                     /locus_tag="ACHW4Z_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8883205.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHW4Z_003753"
     CDS             complement(1033..1803)
                     /locus_tag="ACHW4Z_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883206.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHW4Z_003754"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHW4Z_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHW4Z_003755"
     CDS             complement(2294..2515)
                     /locus_tag="ACHW4Z_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883207.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHW4Z_003756"
     CDS             complement(2516..3199)
                     /locus_tag="ACHW4Z_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8883208.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHW4Z_003757"
     CDS             complement(3263..3574)
                     /locus_tag="ACHW4Z_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883209.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHW4Z_003758"
     CDS             complement(3571..4473)
                     /locus_tag="ACHW4Z_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR8883210.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="ACHW4Z_003759"
     CDS             4913..5872
                     /locus_tag="ACHW4Z_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR8883211.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="ACHW4Z_003760"
     CDS             5872..6267
                     /locus_tag="ACHW4Z_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8883212.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="ACHW4Z_003761"
     CDS             6450..6830
                     /locus_tag="ACHW4Z_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR8883213.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6876..7552
                     /locus_tag="ACHW4Z_003762"
                     /pseudo
     CDS             6876..7552
                     /locus_tag="ACHW4Z_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT         2056 a         1950 c         1847 g         1699 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac catcatcaag cccctgttac ctcctgaacc cgccacacca cgggccggac
     6961 gcccatgggc tgagcaccgt aaaatcatca atggcatgtt ctgggtgtta tgttccagtg
     7021 caccatggcg cgatttaccc gaacgatatg gggcatggaa aactgtttat aaccgcttta
     7081 accgatggtc aaagtcaggc gtgattaata ttattttcaa caggttgctt tcgctacttg
     7141 atgcaaacgg ctttattgac tggtctgcca ccgcgctgga tggcagtaat atccgggcgc
     7201 tgaaatgtgc cgccggtgct caaaaaaaca tcccgatatc gacggagata atgggccggg
     7261 tcgctctcgc ggcggttttg gcaccaaaat ccatctggca acagacggaa gtggcctccc
     7321 gttaaatatc gtgctaagcc ccggacaagc tcatgaaagc cagttcgcgc aacgacttct
     7381 ggacgggatt ggcgttcagc gtcagaacgg cagcatgaaa cgccgtggac atgcggtact
     7441 ggctgataaa gcgtattcag ggcgcgcatt gcgcaacgag ctgaaaaata acggtataaa
     7501 ggcagtaatc ccccgaaagt caaatgagaa aatggcatcg gatggacgtg ca
//
LOCUS       DAZSNG010000158         7537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZSNG010000158 DAZSNG010000000
VERSION     DAZSNG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.161"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1157
                     /gene="rbsA"
                     /locus_tag="ACHW4Z_003763"
                     /pseudo
     CDS             1..1157
                     /gene="rbsA"
                     /locus_tag="ACHW4Z_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1162..2127
                     /gene="rbsC"
                     /locus_tag="ACHW4Z_003764"
     CDS             1162..2127
                     /gene="rbsC"
                     /locus_tag="ACHW4Z_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR8883214.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2152..3042
                     /gene="rbsB"
                     /locus_tag="ACHW4Z_003765"
                     /pseudo
     CDS             2152..3042
                     /gene="rbsB"
                     /locus_tag="ACHW4Z_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3168..4097
                     /gene="rbsK"
                     /locus_tag="ACHW4Z_003766"
     CDS             3168..4097
                     /gene="rbsK"
                     /locus_tag="ACHW4Z_003766"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR8883215.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4101..5093
                     /gene="rbsR"
                     /locus_tag="ACHW4Z_003767"
     CDS             4101..5093
                     /gene="rbsR"
                     /locus_tag="ACHW4Z_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR8883216.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5059..6486)
                     /gene="mdtD"
                     /locus_tag="ACHW4Z_003768"
     CDS             complement(5059..6486)
                     /gene="mdtD"
                     /locus_tag="ACHW4Z_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR8883217.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6509..7201)
                     /locus_tag="ACHW4Z_003769"
     CDS             complement(6509..7201)
                     /locus_tag="ACHW4Z_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR8883218.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1915 a         1829 c         1986 g         1807 t
ORIGIN      
        1 accatgtatg tcgaagcgga taaattgctg gctaaactta acctgcgctt taaaagcgac
       61 aagttagtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc caaagtgctg
      121 agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac cgataccgaa
      181 accgaatccc tgttccgcgt catccgcgag ctgaaatcgc aaggccactg tattgtctat
      241 atctcccacc gcatgaaaga aatcttcgag atttgcgatg acgttaccgt ttttcgtgat
      301 gggcaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct gattgagatg
      361 atggtgggcc gcaagctgga agatcaatat ccacacctgg acaaagcgcc aggagatatc
      421 cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt tactttacgc
      481 aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga actgatgaaa
      541 gtgctctacg gcgcactgcc gcgcaccagc ggttacgtca ccctggatgg gcatgaagtc
      601 gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc cgaagaccgt
      661 aaacgtgacg gtttagtgct gggcatgtca gtaaaagaga acatgtcgct gacagcgctg
      721 cgctacttca gccgcgctgg cggcagtttg aagcatgccg atgaacagca ggctgtgagt
      781 gatttcattc gtctgtttaa tgtgaaaacg ccgtcgatgg aacaggcaat tggtctgctt
      841 tccggtggca atcagcaaaa agtggcgatt gcccgcggtc tgatgacacc cccaaagtat
      901 tgatccttga tgagcctacc cgtggcgtag atgtcggcgc gaaaaaagag atctatcaac
      961 tgattaacca gttcaaagcc gatggcttga gtatcattct ggtgtcatcg gagatgccag
     1021 aagtattagg catgagcgat cgcatcatcg tcatgcatga agggcatctc agcggggaat
     1081 ttactcgtga gcaggccacc caggaagtgt taatggctgt cgctgtgggc aagcttaatc
     1141 gcgtgaatca ggagtaaaaa aatgataacc cagactgtct ctggtcgccg ttatttcacg
     1201 aaagcgtggc tgatggagca gaaatcgctt atcgctctgc tggtgctgat cgcgattgtc
     1261 tcgacgttaa gcccgaactt tttcaccatc aataacttat tcaatattct ccagcaaact
     1321 tcggtgaacg ccattatggc ggtcgggatg acgctggtga tcctgacgtc gggcatagac
     1381 ttgtcggtag gttctctgtt ggcgctaacc ggcgcagttg ctgcatctat cgtcggcatt
     1441 gaagtcaatg cgctggtggc tgtcgctgct gctctcacgt taggtgccgc aattggtgcg
     1501 gtaaccgggg tgattgtagc gaaaggtcgc gtccaggcgt ttatcgctac gctggttatg
     1561 atgcttttac tgcgcggcgt gaccatggtt tataccaacg gtagcccagt gaataccggc
     1621 tttactgaga acgccgatct gtttggctgg tttggtattg gtcgtccgct gggcgtaccg
     1681 acgccagtct ggatcatggg gattgtcttc ctcgcggcct ggtacatgct gcatcacacg
     1741 cgtctggggc gttacatcta cgcgctgggc ggcaacgaag cggcaacgcg tctttctggt
     1801 atcaacgtca ataaaatcaa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg
     1861 gccgggatca ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat
     1921 gagctggatg ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt
     1981 cgcattgttg ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat
     2041 ttgttaggtg tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg
     2101 gtgctggtag acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg
     2161 aaaaaactgg ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg
     2221 atggcaaaag acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg
     2281 ctgaaagatg gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac
     2341 tcccagaaca acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc
     2401 aaaattctgc tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct
     2461 aactaggcga acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg
     2521 agccacattg catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag
     2581 aaagcgggtg aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc
     2641 cgtgaacgtg gcgaaggctt ccagctggcc gttgctgctc acaagtttaa tgttcttgcc
     2701 agccagccag cagattttga tcgcaccaaa ggtttgaacg taatgcagaa cctgttgacc
     2761 gctcatccgg atgttcaggc tgtattcgcg cagaatgatg aaatggcgct gggcgcgctg
     2821 cgcgcactgc aaactgccgg taaatcggat gtgatggtcg tcggatttga cggtacaccg
     2881 gatggcgaaa aagcggtgaa tgatggcaaa ctagcagcga ctatcgctca gctacccgat
     2941 cagattggcg cgaaaggcgt tgaaaccgca gataaagtgc tgaaaggcga gaaagttcag
     3001 gctaagtatc cggttgatct gaaactggtt gttaagcagt agttttaatc aggttgtatg
     3061 acctgatggt gacataaata cgtcatcgac agatgaacgt gtaatataaa gaaaagcagg
     3121 gcacgcgcca ccctaacacg gtggcgcatt ttatggacat cccgaatatg caaaacgcag
     3181 gcagcctcgt tgttcttggc agcattaatg ctgaccacat tcttaatctt caatcttttc
     3241 ctactccagg cgaaaccgta accggtaacc actatcaggt tgcatttggc ggcaaaggcg
     3301 cgaatcaggc tgtggctgct gggcgtagcg gtgcgaatat cgcgtttatt gcctgtactg
     3361 gcgatgacag cattggcgag agcgttcgcc agcagctcgc cactgataac atcgatatta
     3421 ctccggtcag cgtgatcaaa ggcgaatcaa caggtgtggc gctgattttt gttaatggcg
     3481 aaggtgagaa tgtcatcggt attcatgccg gcgctaatgc tgctctttcc ccatcgctgg
     3541 tggaagcgca acgtgagcgt attgccaacg cgtcagcgtt attaatgcag ctggaatcac
     3601 cactcgaaag tgtgatggca gcggcgaaaa tcgcccatca aaataagact atcgttgcgc
     3661 ttaacccggc tccggctcgc gaacttcctg acgaactgct ggcgctggtg gacattatta
     3721 cgccaaacga aacggaagca gaaaagctca ccggtattcg tgttgaaaat gatgaagatg
     3781 cagcgaaggc ggcgcaggta ctgcatgaaa agggtatccg tactgtactg attactttag
     3841 gaagtcgtgg tgtatgggct agcgtgaatg gtgaaggtca gcgcgttcct ggattccggg
     3901 tgcaggctgt cgataccatt gctgccggag atacctttaa cggcgcgtta atcacggcgt
     3961 tgctggaaga aaaaccattg ccagaggcga ttcggtttgc ccatgctgcc gctgcgattg
     4021 ccgtaacacg taaaggcgca caaccttccg taccgtggcg tgaagagatc gacgcatttt
     4081 tagacaggca gaggtgacgc ttggctacaa tgaaagatgt tgcccgtttg gcgggcgttt
     4141 ctacctcaac agtttctcac gttatcaata aagatcgctt cgtcagtgaa gcgattaccg
     4201 ccaaagttga agcggcgatt aaagaactca attacgcgcc atcagctctg gcgcgtagcc
     4261 tcaaactcaa tcaaacacat accattggca tgttgatcac tgccagtacc aatcctttct
     4321 attcagaact ggtgcgtggc gttgaacgca gctgcttcga acgcggttat agtctcgtcc
     4381 tttgcaatac cgaaggcgat gaacagcgga tgaatcgcaa tctggaaacg ctgatgcaaa
     4441 aacgcgttga tggcttactg ttactgtgta ccgaaacgca tcagccttcg cgtgaaatca
     4501 tgcaacgtta tccgacagtg cctaccgtga tgatggactg ggctccgttc gatggcgaca
     4561 gcgatcttat tcaggataac tcgttgctgg gcggagactt agcaacgcaa tatctgatcg
     4621 ataaaggtca tacccgtatc gcctgtatta ccggtccgct ggataaaact ccggcgcgcc
     4681 tgcggttgga aggttatcgg gcggcgatga aacgtgcggg tctcaacatt cctgatggct
     4741 atgaagtcac tggtgatttt gaatttaacg gcgggtttga cgccatgcgc caactgttat
     4801 cacatccgct gcgtcctcag gccgtcttta ccggaaatga cgctatggct gttggcgttt
     4861 accaggcgtt atatcaggca gagttacagg ttccgcagga tatcgcggtg attggctatg
     4921 acgatatcga actggcaagc tttatgacgc caccattaac cactatccac caaccgaaag
     4981 atgaactggg ggagctggcg attgatgtac tcatccatcg gataacccag ccgacccttc
     5041 agcaacaacg attacaactt actccgattc tgatggaacg cggttcggca tagatttacg
     5101 ctgtcttttg atcaaattat taccatcggt tgttttcaga agcatgaaca ttgctgctga
     5161 agcaacagta ataatgccca tcgtgataaa cgtatagtgg aattgttcga cagtcgttgt
     5221 gccttccatc ccttcataaa tgcgaaggac ggccgcactt acagcaacgc ctaaactaat
     5281 cgacagttgc tgcgtgaccg ccagaacact gttaccgctg ctggcgttgt catcggtcag
     5341 atcggcaagt gtgatggtat tcatcgcggt aaactgcgtc gacatagcca tccctaatat
     5401 aaacaacggc aagatcagca tccatatcgc cattgccggt gattgcaaag agaactgagc
     5461 gatcattagc ccaataatca ccgtgatccc cactaatgta tggcgatagc ccagacgacg
     5521 taagacttgg gtaaccatcg attttgcaat aatggaacct aacgctgtcg gtgccatcat
     5581 acagccggca ataaacgcct gataaccaaa tcctacctgt aacatcaatg gcataaggaa
     5641 cggtacacag ccggtcccca gacgggttgc aatattgcct acgataccga tcgagaaagt
     5701 gcgggtttta aataaatcta atgaaattaa tgggtttggc gtacgtcgcg catggagaat
     5761 atagagaagc agtaacccga tgctggtgac aattaccgtc aaggcaatcc agctggcgac
     5821 aatcttttcc ccgaatagct ctattccgct tgaaaagaga acaaggctga ggccaaacag
     5881 caaaaagcca gtgatatcga atctgcgtcg tgcggtggtg aaattgggca tatgtttgcg
     5941 cgcgtaaaga aggcccgcaa tacctatggg gatattaatt aaaaatatcc agtgccaggt
     6001 tgcccaggtg accagcacgc cgccaagaac ggggcctaaa attggcccca ccagacccgg
     6061 catggcgaca aaattcaata ccggaagaag ttcattacga ggataagcac gcagtaaggc
     6121 cggccgagca acaggcatca tcattgcgtc gcctatccct tgaataaccc ggaagacaac
     6181 cagctgtggt agcgaattag aaagtgcgca ggccagagaa cccaacgtga acagactcac
     6241 ggcaagggta aaaatgcgac gcgtaccgaa gcgatcggct agccatccgc ttaccggaat
     6301 aagcatcgcc accgtcagcg tataactgat gatggctgat tgcatcgcga gaggagaacg
     6361 attaaggcta tgagcgattg cgggtaaggc ggtattcaga atagtggcat caagtgcctg
     6421 catgaagaag gccatcgccg cgatccacgg caaacccgac atactacgct tctttttatc
     6481 gctcattcaa tgtcctgtta tcgggttatc acttatcagg tgagcgtagc agcgcctgac
     6541 aagctttaaa tgccgcgtcg ccatcgcttt ggataatcgc atcgacaatc gcctggtgca
     6601 gatccagctt tatcactgtg tcgctggtaa ttgacgtgaa gtaagtgtga taaaccgaat
     6661 ggaatagcga ggcgaatgag gtcaaaaacg gattggcgct catttcatag atatgctcat
     6721 gccaggccat atcgacttcg atccagcgtt cacggcgaaa gttctctttt aatgccgcca
     6781 tttcggccat taacgtattg agatgcgcct tctgttccgc ggtgccaacc gttgctgcca
     6841 gtaggcaggc ttgcggctcc agacagatac gcataaccag aaagtgatcg atgacctgat
     6901 gaaagttctc ttctgttatc caccaagtaa gcaattcctg atcaagaaaa ttccagttcg
     6961 attgtggcat gacccgagta ccaattcgcg gtcgcggtaa aaccatccct tttgccgtta
     7021 acgttttgac cgcttcgcgt accgctgtac gactcactcc aaattgctcg cccagctcaa
     7081 tctcaccggg caaaatggtg ccgggttcat attcaccttt taagatccgt tgcgccagct
     7141 tctcagccag aacatacgaa aggtttttct gtgcagctaa ctgttgtgcg cttaaaggca
     7201 ttacttatct tcctttttct ttttattcct ccttagtatg ccaccaggaa gtgtgattac
     7261 ggttgcaaaa acggcaaatt gcttgtttta tggcacatta acggggtgtt ttgctgaaaa
     7321 aatgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccgggata actccctata
     7381 atacgccacc actgacacgg aacaacggca aatacgccgc cgggtcagcg gggttctcct
     7441 gagaattccg acagaaaaag caaaaataaa tgattgactc tgtagcggga aagcgtatta
     7501 tgcacacccc cccccgcgcc gctgagaaaa agcgaag
//
LOCUS       DAZSNG010000159         7527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZSNG010000159 DAZSNG010000000
VERSION     DAZSNG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.162"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(291..1691)
                     /gene="asnS"
                     /locus_tag="ACHW4Z_003770"
     CDS             complement(291..1691)
                     /gene="asnS"
                     /locus_tag="ACHW4Z_003770"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR8883219.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1860..3062)
                     /gene="pncB"
                     /locus_tag="ACHW4Z_003771"
     CDS             complement(1860..3062)
                     /gene="pncB"
                     /locus_tag="ACHW4Z_003771"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR8883220.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3328..5943
                     /gene="pepN"
                     /locus_tag="ACHW4Z_003772"
     CDS             3328..5943
                     /gene="pepN"
                     /locus_tag="ACHW4Z_003772"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR8883221.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6150..6917)
                     /gene="ssuB"
                     /locus_tag="ACHW4Z_003773"
     CDS             complement(6150..6917)
                     /gene="ssuB"
                     /locus_tag="ACHW4Z_003773"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8883222.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6914..>7527)
                     /gene="ssuC"
                     /locus_tag="ACHW4Z_003774"
     CDS             complement(6914..>7527)
                     /gene="ssuC"
                     /locus_tag="ACHW4Z_003774"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR8883223.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1913 a         1999 c         1908 g         1707 t
ORIGIN      
        1 taaacggaaa ttttgtttct gatggaaact ttatcgacct ggcacaaaat cctctttcag
       61 acatccagaa tgccaaaaaa tagtatgaaa attgtgctat cagctaagaa aaaactaatc
      121 cgcaataaga gtacgggtta ttttttgtgc gtaaatgttg caatgtaata ttccgtaaca
      181 ggatgatcgt tatggcgggg gtgcaaatag attgcttgac ttaacaaaca gataacttga
      241 cagaaaggat aaaaaagagc cagcaggcgc tggctctttg aggaagaaaa tcagaagctg
      301 gcgttgcgcg gagtacgtgg gaacggaatc acatcacgca cgttttgcac gccagttacg
      361 taagcaatca ggcgttcaaa accaagaccg aaacctgaat gcggaacagt accgtagcga
      421 cgcagatcgc gataccacca gtaatcttct ttattcaggc ccatttccag catacgctcg
      481 tccagaacgt ccagacgttc ttcacgctgg gagccaccaa tgatctcacc gatgcccgga
      541 gccagaacgt ccatagccgc aacggtttta ccgtcttcgt tgaggcgcat atagaacgct
      601 ttaatatctt tcggatagtt tttaaccact accggtgctt taaagtgttc ttccgccaga
      661 taacgctcat gctcagaaga gagatcgacg ccccagtaaa ccgggttttc aaacttcctg
      721 ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca cctgcgcgaa atcggcttca
      781 atgaagcgtt ccagacgtga aacggcatct ttatctacgc gttcagcgaa gaatttcatg
      841 tcgtctgcgc gttcttcgag aaccgctttg aagacatatt tcagcatggc ttcagccaga
      901 cccgcgatat cgttcaggtt agcaaacgcc acttccggct ccagcatcca gaattccgcc
      961 aggtgacggc tggtgttgga gttttcagca cggaaagtcg ggccgaaggt ataaattttg
     1021 gataatgcgc aagcgtaggt ttcgccgttc aactggccag atacggtcag gaaagactct
     1081 ttaccaaaga agtctttgtc gaaatccact ttgccctgat cgttacgcgg caggttttcc
     1141 agatccagcg tagaaacgcg gaacatttcg cctgcacctt cggtatcaga tgcggtaatc
     1201 agtggcgttg aaacccagaa gaatccctgc tcgttaaaga agcgatgcag cgcctgcgcc
     1261 agcgtatggc gaacgcgcgc gacggcacca atcaggtttg tgcgcggacg caggtgagcg
     1321 acttcacgca gatactcaat gctgtggcgt tttgccgcca tcgggtaagt gtctggatct
     1381 tcaacccaac cagcaacttc aaccttgctg gcctgaattt caaattgttg cccctggccc
     1441 ggcgatgcca cgactttacc cgtcacaatg accgagcagc cggtggtcag acgcaggacg
     1501 tcttcattgt aattgggcag agaattattg atgacagcct gtacaggatc aaagcaggaa
     1561 ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat ctcggcgggt acgtacccat
     1621 ccgcgtacgg tgacttcgct gtcaacggct acgcggccct ggagtacgtc ggctacaggc
     1681 acaacgctca taatattctc tctgttaata gtcggaaaaa ataaacactt gtccacccgt
     1741 aaatgggggg gatacctatg ttacctggca tctgcaatca gacaagcaga attcgcaaat
     1801 gcagcgaaag atttcggaat taaaggtaaa aaagggagcc gattagctcc ctgatgatat
     1861 taactggctt ttttaatatg cggaaggtcg aacgctttgc gcagcgcccg aacaaacgct
     1921 ttatcatggc agatagtttt gccagggctg tcagaaagtt tcgccaccgg tttaccgtta
     1981 cactctacca acttaatcac aatattcagg ggttttacct gggggatatc gcaggtcagg
     2041 cgagtcccaa taccaaaact taattgcacg cgggaagaga agtggcggta tagctcaacc
     2101 gctttgcgta aatccagatt gtcagagaaa accagcgttt tactctgtgg atcaattccc
     2161 agcttttcat aatgtgcaat ggctttttca ccccattcaa ccgggtcgcc agagtcatga
     2221 cgcaggccct gataccgact agcgaactcg acaccgaaat cacgcaggaa agcatccata
     2281 gtgatgcagt cggttaatgc aatgccaagt tggtcgggat actcttccag ccaggcagca
     2341 agtgcagctc gctggctgtt ggctagatcc gggctgattt gctgatgtgc ctggaaccat
     2401 tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac gcgccagatc gtagttgctg
     2461 gtgcccacga accaggattc ctgttgcaga cgcttaacga tggtttcttg tacttcgcga
     2521 gaaaaacggc gacgggtgcc aaaatccatc agatggaagc gcgacatatc aagaccggcg
     2581 gttaacgccg agaagtcgac taatttgctt tccagcgtgt cgagggcttg cgcaacgtcg
     2641 gcctgcggtg agcgatagcg atgtaccatt tcactgataa ccgccagcaa aggaacttcc
     2701 cagaggatga cttcacgcca cggaccgctt aagcgaatat ccagcttgcc attatcgttg
     2761 gacacggtga cttgttccgg gttaaagcgg aactcgcgta accagttaag atagtcggcc
     2821 ttaaagaaag gcagggcaga gagccactga tattcatcat cctgcaggcg caggtgctgc
     2881 atcgcctgaa tctgttcacg aatagcatcg gcataaatac ccagcagatc gtcacctcgg
     2941 caacgaaact ccgccgcgac atgcacatcg taatagtgat gaaacacggc ttgctgcata
     3001 tgcaacttat aagcatctgt atccagcaac gagtgcagaa caggagaagc gaattgtgtc
     3061 ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta caataaacat cttcaggaac
     3121 tgctggagta taccttgttt cgcaatttat tgaaccccga tcacaccata tgccaccttt
     3181 ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta gcaataaagg cgtttgtacc
     3241 tgaaaagatg aagattctgc atagcgcgat ttacgcaaca ggaatagact gaacaccaga
     3301 ctctataaaa gatgctaaag gttatttatg actcaacagc cacaagccaa ataccgtcac
     3361 gattatcgtg cgccggatta ccagattact gatattgact tgacctttga cctcgacgcg
     3421 caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc atggtgcatc agatgctccg
     3481 cttcgtctca acggcgaaga cctcaaactg gtttctgttc atattaatga tgagccgtgg
     3541 accgcctgga aagaagaaga agagggcgca ctggtcatca gtaatttgcc ggagcgtttt
     3601 acgcttaaga tcattaatga aataagcctg gcggcgaata cggcgctgga agggctttat
     3661 cagtcaggcg atgcgctttg cacccagtgt gaagccgaag gtttccgcca tattacgtat
     3721 tatctcgacc gcccggacgt gctggcgcgt tttaccacca aaattattgc cgataaaatc
     3781 aaatatccct tcctgctttc caatggtaac cgcgttgcgc aaggcgaact cgaaaacgga
     3841 cgccattggg tacagtggca ggacccgttc ccgaaaccgt gctacctgtt tgcgctggtg
     3901 gcaggcgact ttgatgtact gcgcgatacc tttaccacgc gttctggtcg cgaagtagca
     3961 ctggagctgt acgtcgatcg cggcaacctt gatcgcgcgc cgtgggcgat gacctcgctg
     4021 aaaaactcca tgaaatggga tgaagaacgc tttggcctgg agtatgacct cgacatctat
     4081 atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg agaataaggg tctgaatatc
     4141 tttaactcca aatatgtgct ggcccgcacc gacaccgcca ccgacaaaga ttacctcgat
     4201 attgaacgcg ttatcggcca tgaatatttc cataactgga ccggtaaccg agtgacctgc
     4261 cgcgactggt tccagctcag cctgaaagaa ggtttaaccg tcttccgcga tcaggaattc
     4321 agctctgacc ttggttcccg cgcagttaac cgcatcaata atgtgcgcac catgcgcgga
     4381 ttgcagtttg cggaagacgc cagcccgatg gcgcacccaa tccgcccgga catggtcatt
     4441 gagatgaaca acttctacac cctgaccgtt tacgagaagg gcgcggaagt gatccgtatg
     4501 atccacaccc tgctgggcga agaaaacttc cagaaaggga tgcaactcta tttcgagcgt
     4561 catgatggta gcgcagcgac ctgtgacgac tttgtgcagg cgatggaaga tgcgtcgaat
     4621 gttgatctct cccatttccg ccgttggtac agccagtccg gtacgccggt tgtgaccgtc
     4681 aaagacgact acaatccgga aactgagcag tacaccctga ccatcagcca gcgcacgcca
     4741 gccacgccgg atcaggcaga aaaacagccg ctgcatattc cgtttgccat cgaattgtat
     4801 gacaacgaag gtaaagtgat cccgttgcag aaaggcggtc atccggtgaa ttccgtgctg
     4861 aacgtcaccc aggcggaaca gacctttgtt tttgataatg tctacttcca gccggtgcct
     4921 gcgctgctgt gcgaattctc tgcgccagtg aaactggaat ataagtggag cgatcagcaa
     4981 ctgaccttcc tgatgcgtca tgcgcgtaat ggtttctccc gttgggatgc ggcgcaaagt
     5041 ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc agcaagggca gccgctgtct
     5101 ctgccggtac atgtggctga cgctttccgc gcggtgctgc tcgatgagaa gattgatccg
     5161 gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg aaatggctga actgttcgat
     5221 atcatagacc cgattgctat tgccgaagta cgcgaagcac tcactcgtac tctggcgact
     5281 gaactggcgg atgagctgct ggctatttac aacgcgaatt accagagcga gtaccgtgtt
     5341 gagcatgaag atattgcaaa acgcactctg cgtaatgcct gcctgcgctt ccttgctttt
     5401 ggtgaaacgc atctggctga cgtgctggtg agcaagcaat accatgaagc aaacaatatg
     5461 accgatgcgc tggcagcgct ttctgcggcg gttgctgcac agctgccttg ccgtgacgcg
     5521 ctgatgcagg agtacgacga taagtggcat caggacggtc tggtgatgga taaatggttt
     5581 atcctgcaag ccaccagccc ggcggcgaat gtgctggaga cggtgcgtgg tctgttgcag
     5641 catcgctcat ttaccatgag caacccgaac cgtattcgtt cgttgattgg tgcgtttgcg
     5701 ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg gttaccagtt cctggtggaa
     5761 atgcttaccg acctcaacag ccgtaacccg caggtagctt cacgtctgat tgaaccgctg
     5821 attcgcctga aacgttatga tgccaaacgt caggagaaaa tgcgcgcggc gctggaacag
     5881 ttgaaagggc tggaaaatct ctctggcgat ctttacgaga agataactaa agcactggct
     5941 tgatactttt gagccaaaat gcctgatgcg acgcttgacg cgtcttatca ggcctacaac
     6001 tccgttgaaa ttgctggttt tgtaggctgg ataaggcgtt cacgccgcat ccggcagccg
     6061 tgcccaactg cctgatgcga tgcttgacgt gtcttatcag gcctacaagt ccgttgaaaa
     6121 tgctggtttt gtgcgccaca tccggcatat taaccatgac gccgaatcag ttgttcagaa
     6181 tgcccacgcc gcattacccg ttgcaatact tccgcttcca gctccgctaa tcgcaccgac
     6241 cctaatcgac gcggacgggg gatatccact gtcaaatcca gactaatttt tccctcttca
     6301 attaacaaca cccggtcagc cattgctacc gcttcgctca catcatgcgt caccaacagt
     6361 acggtaaagc cgtgctgctg ccaaagtgac acaatcaaat cctgcatctc gagtcgcgtt
     6421 aaggcgtcca gtgccccgag cggttcatca agcaacaata aacccggtcg atgaatcaac
     6481 gctcttgcca gcgccacacg ctgtttctgc ccgccagaaa gtgcggcagg ccattccccg
     6541 gcgcgattct ccagtcctgc cgcagccaac gcccgacgcg cggcatcgcg ccactgacct
     6601 ttaaggccta acccaacgtt atcaatcacc gatttccacg gcagcagacg cgcatcctga
     6661 aacatcattc gcgtatcatc ctgaatttca gccagcggtg tggtgcccgc taacacatcg
     6721 cctgcggttg gcgtttccag accggccagc aggtgcagca gggtactttt accaccaccg
     6781 ctgcggccca ccaccgccac aaactgacct gccggaatat gtaaatccag ttggttcagg
     6841 acgatatttt ccgcgtaatg tttgcttact gcattgagca acaatggtgt gccctggttc
     6901 agacgagcag tattcatatc gtggcctcct tcaaatgata agccgggttc cagcgcaacc
     6961 agaggcgctc taacagctgc gcgctgacgt cagcaagttt gccgagcagg gcgtaaagaa
     7021 taatagcgac taccaccacg tctgtttgca aaaactctcg cgcattcatc gccagataac
     7081 caatgccgga attggcagaa atggtttcgg caacaatcag cgtcagccac atcaggccca
     7141 acgcaaaacg cacgccgacc ataattgagg gcagggcatc gggcaggatc acatggataa
     7201 acagcggtat gccggataat ccatagctac gcgccatctc caccagcccg cgatcgatat
     7261 tacggatgcc atgccaggtg ttgatataaa tggggaacag cgtaccgagc gccaccagaa
     7321 agattttcgc ggattcatca atgccaaacc acaaaatcac cagcgggatc agcgccagat
     7381 gcggcacgtt gcgcaacatc tgaattgagg tatccagcag cctttctccc cagcgcgaca
     7441 gtccgctaat cagtcccaga atcaggccca gcgatccgcc aattgaaaag ccaatcagcg
     7501 cccgccagga actgatcgcc agatgct
//
LOCUS       DAZSNG010000160         7375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZSNG010000160 DAZSNG010000000
VERSION     DAZSNG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.163"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1541
                     /locus_tag="ACHW4Z_003775"
     CDS             <1..1541
                     /locus_tag="ACHW4Z_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR8883224.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHW4Z_003776"
     CDS             1593..2750
                     /locus_tag="ACHW4Z_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8883225.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHW4Z_003777"
     CDS             complement(2716..2838)
                     /locus_tag="ACHW4Z_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883226.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHW4Z_003778"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHW4Z_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHW4Z_003779"
     CDS             4764..7124
                     /locus_tag="ACHW4Z_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8883227.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="ACHW4Z_003780"
     CDS             7181..>7375
                     /locus_tag="ACHW4Z_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883228.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1615 c         1568 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZSNG010000161         7365 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZSNG010000161 DAZSNG010000000
VERSION     DAZSNG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.164"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            107..1006
                     /gene="lysO"
                     /locus_tag="ACHW4Z_003781"
     CDS             107..1006
                     /gene="lysO"
                     /locus_tag="ACHW4Z_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR8883229.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1150..2802
                     /gene="hcp"
                     /locus_tag="ACHW4Z_003782"
                     /gene_synonym="priS"
     CDS             1150..2802
                     /gene="hcp"
                     /locus_tag="ACHW4Z_003782"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR8883230.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2814..3782
                     /gene="hcr"
                     /locus_tag="ACHW4Z_003783"
     CDS             2814..3782
                     /gene="hcr"
                     /locus_tag="ACHW4Z_003783"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR8883231.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3915..5663
                     /gene="poxB"
                     /locus_tag="ACHW4Z_003784"
     CDS             3915..5663
                     /gene="poxB"
                     /locus_tag="ACHW4Z_003784"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR8883232.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            5700..6701
                     /gene="ltaE"
                     /locus_tag="ACHW4Z_003785"
     CDS             5700..6701
                     /gene="ltaE"
                     /locus_tag="ACHW4Z_003785"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR8883233.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6712..>7365
                     /locus_tag="ACHW4Z_003786"
     CDS             6712..>7365
                     /locus_tag="ACHW4Z_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR8883234.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     "
BASE COUNT         1653 a         1941 c         2000 g         1771 t
ORIGIN      
        1 ctctgatgca taacatttcc ttttaattgc aactttactc gcccttccgc tttcagtagt
       61 gtgtcatcgc gcatttcgac ttcttctttg caaggactgg attttcatgt tttctgggct
      121 gttaatcatt ctggttcccc tgattgtggg ttacctcatt ccgcttcgcc aacaagctgc
      181 gttaaaagct attaatcagc tattaagctg gatggtttac cttattctct tttttatggg
      241 tatcagtctg gcgtttctcg ataacctcgc cagtaacctg ttggcgattc tgcattattc
      301 tgccgtcagt attaccgtta ttttactgtg taatattgcc gccctgatgt ggctggagcg
      361 aggcctgccg tggcgcaacc accatcagca agaaaaactc ccgtcgcgta ttgcgatggc
      421 gctggagtcg ctaaaactgt gcggcgtagt agtgattggt tttgccattg gtctgagtgg
      481 actggctttc ttacaacacg cgaccgaagc cagtgaatac acgttaattt tgctactttt
      541 cctcgttggt attcagttgc gcaataatgg catgacctta aagcagattg tccttaatcg
      601 ccggggaatg attgtcgccg tggtggtggt tgtcagttca ttaattggtg gtttaattaa
      661 cgcctttatt cttgatctcc ccatcaatac cgcgctggca atggcctccg gtttcggctg
      721 gtattctctt tccggtattt tattgaccga atcttttggt ccggtaatcg ggagcgcggc
      781 gttttttaat gatctggccc gtgaactgat tgctattatg ttgatccctg ggctgattcg
      841 ccgcagccgc tctactgcac tgggcttatg cggtgccaca tcaatggatt tcaccctgcc
      901 cgttcttcaa cgtactggcg ggctggatat ggtcccggcg gcaattgttc acggttttat
      961 tcttagcctg ttagtgccga tcctcatcgc ctttttctct gcgtaatacc tctctggcgg
     1021 tagttccctg ccgccaaaat tgcgctaaat caatctccct taaagttgca tgaaaaatcc
     1081 cttttatccc cgcgttaagc gtcttaccct taaacatgta tattaaatat aactttaaaa
     1141 ggtgtgatca tgttttgtgt gcaatgtgaa caaactatcc gtactccggc agaaaacggc
     1201 tgctcatacg cgcaggggat gtgtggtaaa acggcggaaa cttctgacct tcaggattta
     1261 ctcatcgcgg cgctgcaagg gctttctgcc tgggcggtaa aagcgcgtga atacggcatc
     1321 atcaaccacg atgttgacag cttcgcgcca cgtgcctttt tctcaaccct gaccaacgtt
     1381 aacttcgatt ctccgcgtat tgtcggctac gctcgtgaag cgattgcctt gcgcgaggcg
     1441 ctgaaagcac aatgcctggc tgtagatgcc aacgcgcgcg tcgataaccc gatggctgac
     1501 ctgcaactgg tgagcgatga tctcggcgag ctgcaacgtc aggcagcaga atttacccct
     1561 aacaaagata aagcggcgat tggcgaaaac attctcggcc tgcgtctgct gtgcctgtat
     1621 ggcctgaaag gtgcggcggc ctatatggaa cacgcgcatg ttctcggtca atacgacaac
     1681 gatatttatg cccagtacca taaaatcatg gcgtggctgg ggacctggcc tgccgatatg
     1741 aacgcgcttc ttgagtgttc aatggaaatc ggccagatga acttcaaagt gatgagcatt
     1801 ctggatgcag gcgaaaccgg taaatacggt cacccgacgc caacccaggt caacgtcaaa
     1861 gcgacggcgg gtaaatgcat tctgatttcc ggtcacgatc tcaaagatct ctacaacctg
     1921 ctggaacaga ccgaaggcac gggcgttaat gtctacaccc acggcgaaat gctgcctgcg
     1981 catggctacc cggagctgcg taaattcaag catctggtcg gtaactacgg cagcggctgg
     2041 cagaatcagc aagtggagtt cgctcgtttc cctggcccca tcgtgatgac ctcgaactgc
     2101 atcatcgacc caaccgtagg cgcttatgac gatcgtatct ggacccgcag catcgttggc
     2161 tggcctggcg tgcgtcatct ggatggcgaa gatttctctg cggtcatcgc ccaggcgcaa
     2221 caaatggcgg gcttcccgta cagcgaaatt cctcacctga tcaccgtggg ctttggtcgc
     2281 cagacgctgc ttggtgcagc cgatacgctg attgatctgg ttagccgtga aaaactgcgt
     2341 catatcttcc tgcttggtgg ctgtgacggc gcacgcggcg agcgccacta cttcaccgat
     2401 ttcgccacca gcgtgccgga tgactgcctg atcctgaccc tcgcctgtgg taaatatcgc
     2461 tttaacaaac ttgagtttgg cgatatcgaa ggtctgccgc gtctggtaga tgcaggtcaa
     2521 tgtaacgatg cttactcagc aattattctg gctgtcactc tggcagagaa actgggctgt
     2581 ggtgtgaacg atctgccgct gtcgctggtg ctctcctggt ttgaacagaa agcgattgtc
     2641 attctgctga cgctgctttc tctgggcgtg aaaaatatcg tcaccggccc gactgcacct
     2701 ggtttcctga caccagacct gctggcggtg ctgaacgaga aattcggcct gcgttctatc
     2761 accactgttg aagaagacat gaagcaactg ttgaacgcgt aaggagtttg tcgatgacga
     2821 tgccaacgaa tcaatgcccg tggcggatac aggttcatca cattacgcaa gaaacgccgg
     2881 atgtgtggac gatttccctg atttgccacg atcactaccc atatcgcgcc gggcaatatg
     2941 cactggtcag cgtgcgaaac tcagcggaaa cgctgcgtgc ttacaccatt tcctccacgc
     3001 caggcgtgag tgaatatatc accctgaccg tgcggcggat tgatgacggt gtcggctccc
     3061 agtggctgac gcgcgatgta aaacgcggtg attatctctg gctttcggac gcgatggggg
     3121 aatttacctg cgacgataaa gcagaagata aattcctgtt gctggcggca ggctgcggcg
     3181 tcacgccgat tatgtcgatg cgtcgctggc ttgcgaagaa ccgtccacag gccgatgtgc
     3241 gggtgatcta caacgtgcgt acgccgcagg atgttatttt cgccgatgag tggcgtaact
     3301 atccggtaac gctggtggca gaaaataacg ttaccgaagg ctttatcgct ggtcgcctca
     3361 ctcgcgaact gctggcagat gtacctgact tagcttcacg taccgtgatg acctgcggcc
     3421 cggctccgta tatggattgg gtagagcagg aagtgaaagc gctcggcgtg acgcgtttct
     3481 ttaaagagaa attcttcacc ccagtagcag aagcggcgac cagcggtctg aaattcacca
     3541 aactgcaacc ggcacgagaa ttttacgccc cggttggcac cacgctactg gaggcgctgg
     3601 aaagcaataa cgttccggtt gtcgctgcct gccgtgcggg tgtttgcggc tgctgtaaga
     3661 caaaagtggt ttccggtgaa tatacggtga gcagtacaat gacgctgacc gacgccgaaa
     3721 tcgctgaagg ttacgtactg gcctgctcct gccatccgca gggggatttg gttctcgcat
     3781 aatcgcctta tgcccgatga tattcctttc atcgggctat ttaaccgtta gtgcctcctt
     3841 tctctcccat cccttccccc tccgtcagat gaactaaact tgttaccgtt atcacattca
     3901 ggagatggag aaccatgaaa caaacggttg cagcttatat cgccaaaaca ctcgaatcgg
     3961 caggcgtgaa acgcatctgg ggagtcacgg gcgactctct gaacggtctt agcgacagtc
     4021 ttaatcgcat gggcaccatc gagtggatgt ccacccgcca tgaagaagtg gcggcgtttg
     4081 ccgctggcgc tgaagcacaa ctttcaggag aactggcggt ctgtgccgga tcgtgcggcc
     4141 caggcaacct gcacttgatc aacggcctat tcgattgcca ccgcaataac gttccggtac
     4201 tggcgattgc cgctcatatt ccctccagcg aaattggcag cggctatttc caggaaaccc
     4261 acccacaaga gctattccgc gaatgtagtc actattgcga gctggtttcc agcccggagc
     4321 agatcccaca agtgctggca attgctatgc gcaaagcggt gcttaaccgt ggcgtttccg
     4381 ttgttgtatt accgggcgac gtggcgttaa aacctgcgcc agaaggggca accacgcact
     4441 ggtatcatgc gccacagcca gtcgtgatgc cggaagaaga agagttacgc aaactggcgc
     4501 aactgctgcg ttattccagc aatatcgccc tgatgtgtgg cagcggctgc gcgggggcgc
     4561 ataaagagtt agttgagttt gccgggaaaa ttaaagcacc tattgttcat gccctacgcg
     4621 gtaaagagca tgtcgaatac gataatccgt atgatgttgg aatgaccggg ttaatcggat
     4681 tctcgtcagg tttccatacc atgatgaacg ccgacacgtt agtgctactc ggtactcagt
     4741 tcccctaccg tgcgttctac ccgaccgatg ccaaaattat tcagattgat attaatccag
     4801 ccagcatcgg cgcgcatagc aaggtagata tggcgctggt cggcgatatc aaatcaaccc
     4861 tgcgcgcatt gctgccactg gtggaagaaa aaaccgatcg caagtttctg gataaagcgc
     4921 tggaagatta ccgcgatgcc cgcaaagggc tggaagatta ccgcgatgcc cgcaaagggc
     4981 tggacgattt agctaaaccg agcgagaaag ccattcaccc gcaatatctg gcgcagcaaa
     5041 ttagtcattt tgccgccgat gacgccatct ttacctgtga cgtcggcacg ccaacggtat
     5101 gggcggcacg ttatctgaaa atgaacggca agcgtcgcct gttaggttcg tttaaccacg
     5161 gttcgatggc taacgccatg ccgcaggcgt tgggtgcgca ggcaaccgag ccggaacgtc
     5221 aggtggtcgc catgtgcggc gatggcggtt ttagcatgtt gatgggcgat ttcctctcag
     5281 tagtgcagat gaaactgccg gtgaaaatta tcgtctttaa taacagcgtg ctgggctttg
     5341 tggcgatgga gatgaaggcc ggaggttacc tgacagacgg tactgagctg cacgacacca
     5401 actttgcccg cattgccgaa gcgtgcggca ttacgggtat ccgtgtagaa aaagcctctg
     5461 aagttgatga agccctgcaa cgcgccttct ctatcgacgg tccggtgttg gtggatgtgg
     5521 tggtcgccaa agaagaatta gccattccac cgcagatcaa actcgaacag gccaaaggtt
     5581 ttagcctgta tatgttgcgc gcaattatca gcggacgcgg cgatgaagtg atcgaactgg
     5641 cgaaaacgaa ctggctaagg taaaaagggt ggcatttccc gtcataataa ggacatgcca
     5701 tgattgattt acgcagtgat accgttaccc gaccgagccg cgccatgctc gaagcgatga
     5761 tggccgcccc ggttggggac gacgtttacg gagacgaccc taccgttaat gctctgcagg
     5821 actatgcagc agagctttcc ggtaaagaag ccgccatttt tctgcctacc ggcactcagg
     5881 ccaacctggt cgctctgctc agtcactgcg aacgtggcga agagtatatt gtcggtcagg
     5941 ccgcgcataa ctatctgttt gaagccggtg gcgcagcggt gctgggcagt attcagccgc
     6001 aacccatcga cgcggctgcc gacggcacgc taccgctgga taaagtggcg atgaaaatca
     6061 aacccgacga tatccatttc gcccgcacca aattactcag tctggaaaac acccacaacg
     6121 gcaaagtgct gccgcgtgaa tacctgaaag aagcatggga atttacccgc gagcgcaatc
     6181 tggcgctgca tgttgacggt gcgcgcatct ttaatgccgt ggtggcttac ggctgcgaac
     6241 tgaaagagat cacgcaatat tgtgattcgt tcaccatttg cctgtcgaaa ggtcttggga
     6301 cgccagtcgg ttcattactc gtcggtaatc gtgattacat taaacgtgcc atccgctggc
     6361 ggaaaatggc cggtggcggg atgcgtcagt ccgggattct ggctgccgcc gggatgtatg
     6421 ccctggaaaa taacgtcgca cggttgcagg aagatcacga caacgccgcc tggatggcgg
     6481 agcaactgcg tgaagcaggc gcggatgtga tgcgtcagga cactaatatg ctgtttgttc
     6541 gcgtcgggga agaaaatgct gccgcgttag gcgaatacat gaaagcgaga aacgtgctga
     6601 ttaacgcctc gccgattgtc cgcctggtga cgcatcttga cgtctcgcgc gaacaactgg
     6661 cggaagtcgc cgcccactgg cgcgcattcc tggcgcgtta aggagagaga cgtgccgcaa
     6721 cgcattttag ttctcggtgc cagtggctac attggtcagc atctggtgcg cacactcagc
     6781 cagcaagggc atcagatcct agcggcggca cgtcatgtcg acaggcttgc aaagctgcaa
     6841 ctggcaaacg tcagttgcca taaagtcgat ctcagctggc cggataacct tccggccctg
     6901 ttgcaggata tcgataccgt ctattttctg gtgcacagca tgggcgaagg cggcgatttt
     6961 atcgctcagg agcgccagct ggctctcaac gtccgcgatg cgctacgtga agtaccagtt
     7021 aagcaattaa tctttctcag ttcgttgcag gccccgccac atgagcagtc ggatcatctg
     7081 cgagctcgtc aggctacggc ggacattctt cgtgaagcga atgtaccagt gaccgaactt
     7141 cgggccggaa ttatcgttgg cgcaggttca gcggcgttcg aagtcatgcg cgatatggtc
     7201 tacaacctgc cagtgttaac gccgccacgc tgggtacgtt cacgcaccac gcccatcgcg
     7261 ctggaaaact tgctgcacta tctggtggcg ctgttagacc atccagccaa cgaacaccgc
     7321 atcttcgaag ccgccggacc agaggtgctc agttatcagc aacag
//
LOCUS       DAZSNG010000162         7314 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZSNG010000162 DAZSNG010000000
VERSION     DAZSNG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.165"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..428
                     /gene="ipgA"
                     /locus_tag="ACHW4Z_003787"
     CDS             39..428
                     /gene="ipgA"
                     /locus_tag="ACHW4Z_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR8883235.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            444..1070
                     /gene="ipgB1"
                     /locus_tag="ACHW4Z_003788"
     CDS             444..1070
                     /gene="ipgB1"
                     /locus_tag="ACHW4Z_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR8883236.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            1127..1594
                     /gene="ipgC"
                     /locus_tag="ACHW4Z_003789"
     CDS             1127..1594
                     /gene="ipgC"
                     /locus_tag="ACHW4Z_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR8883237.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            1600..3342
                     /gene="ipaB"
                     /locus_tag="ACHW4Z_003790"
     CDS             1600..3342
                     /gene="ipaB"
                     /locus_tag="ACHW4Z_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR8883238.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            3305..4453
                     /gene="ipaC"
                     /locus_tag="ACHW4Z_003791"
     CDS             3305..4453
                     /gene="ipaC"
                     /locus_tag="ACHW4Z_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR8883239.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            4504..5502
                     /gene="ipaD"
                     /locus_tag="ACHW4Z_003792"
     CDS             4504..5502
                     /gene="ipaD"
                     /locus_tag="ACHW4Z_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR8883240.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            5511..>7314
                     /gene="ipaA"
                     /locus_tag="ACHW4Z_003793"
     CDS             5511..>7314
                     /gene="ipaA"
                     /locus_tag="ACHW4Z_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR8883241.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKN"
BASE COUNT         2723 a         1448 c         1198 g         1945 t
ORIGIN      
        1 tgaataactc tcattctaat atatagaagg ccatagaaat gtgtcgcaaa ctatatgata
       61 aactttatga aataacaggt gcaaagcttg attttaacga taaaaatcaa gcctttatat
      121 tgctcgaaga acaaatacct gtttgtataa cagataatga tgaatacatc tttttgaccg
      181 gacttcttaa tgaacatgaa ctttttacag aaaatattat taatccagaa cacatactca
      241 tccttaacta ctctttgagt agagattatg gttcttctat ctgtcttctc cctgatactc
      301 atcaatgtgt tttgaccaag aaacactaca aaaaatatct ttcacctgat gaactcattg
      361 aatctttgta tgaattttta ttttgtatta aactaaccat tgcaaacatc acttcagaag
      421 tgaactaaca tatagggggt atcatgcaaa ttctaaacaa aatacttcca caggttgaat
      481 tcgcgattcc tcgcccatca tttgattcgt taagtcgcaa taagcttgta aagaaaatat
      541 tatctgtttt taacttaaaa caacgctttc ctcagaagaa ttttggatgc ccggtgaata
      601 taaataaaat cagggacagc gtcatagaca aaataaaaga tagtaattcg gggaatcaac
      661 tattttgttg gatgagccaa gagcgaacca cttatgtctc ttcaatgata aacagaagta
      721 tagatgaaat ggccatacac aatggagttg ttttgacctc agataacaaa aaaaacatat
      781 ttgcagctat tgaaaaaaaa ttcccggata taaaacttga tgaaaaatca gcacaaacat
      841 ctatcagtca tacagcacta aacgagattg cctcatctgg cctcagagca aagattttaa
      901 aacgctatag cagtgacatg gatttgttta acacacaaat gaaggatctt acaaatctag
      961 tatccagttc cgtctacgat aaaatattta atgaatcaac aaaagtttta caaatagaaa
     1021 taagcgcaga ggttctaaag gctgtatacc gtcaaagcaa tacaaattaa atcttttata
     1081 ttaaatctta tacttattaa agtaaagcag acaaaaagga gaccttatgt ctttaaatat
     1141 caccgaaaat gaaagcatct ctactgcagt aattgatgca attaactctg gcgctacact
     1201 gaaagatatt aatgcaattc ctgatgatat gatggatgac atttattcat atgcttatga
     1261 cttttacaac aaaggaagaa tagaggaagc tgaagttttc ttcaggtttt tatgtatata
     1321 cgacttttac aatgtagact acattatggg actcgcagct atttatcaga taaaagaaca
     1381 gttccaacaa gcagcagacc tttatgctgt cgcttttgca ttaggaaaaa atgactatac
     1441 accagtattc catactggac aatgccagct tcggttgaaa gcccccttaa aagctaaaga
     1501 gtgcttcgaa ctcgtaattc aacacagcaa tgatgaaaaa ttaaaaataa aagcacaatc
     1561 atacttggac gcaattcagg atatcaagga gtaattatta tgcataatgt aagcaccaca
     1621 accactggtt ttcctcttgc caaaatattg gcttccactg agcttggaga caatactatc
     1681 caagctgcaa atgatgcagc taacaaatta ttttctctta caattgctga tcttactgct
     1741 aaccaaaata ttaatacaac taatgcacac tcaacttcaa atatattaat ccctgaactt
     1801 aaagcaccaa agtcattaaa tgcaagttcc caactaacgc ttttaattgg aaaccttatt
     1861 caaatactcg gtgaaaaatc tttaactgca ttaacaaata aaattactgc ttggaagtcc
     1921 cagcaacagg caagacagca aaaaaaccta gaattctccg ataaaattaa cactcttcta
     1981 tctgaaactg aaggactaac cagagactat gaaaaacaaa ttaataaact aaaaaacgca
     2041 gattctaaaa taaaagacct agaaaataaa attaaccaaa ttcaaacaag attatccgaa
     2101 ctcgacccag agtcaccaga aaagaaaaaa ttaagccggg aagaaataca actcactatc
     2161 aaaaaagacg cagcagttaa agacaggaca ttgattgagc agaaaaccct gtcaattcat
     2221 agcaaactta cagataaatc aatgcaactc gaaaaagaaa tagactcttt ttctgcattt
     2281 tcaaacacag catctgctga acagctatca acccagcaga aatcattaac cggacttgcc
     2341 agtgttactc aattgatggc aacctttatt caactagttg gaaaaaataa tgaagaatct
     2401 ttaaaaaatg atctggctct attccagtct ctccaagaat caagaaaaac tgaaatggag
     2461 agaaaatctg atgagtatgc tgctgaagta cgtaaagcag aagaactcaa cagagtaatg
     2521 ggttgtgttg ggaaaatact tggggcactt ttaactatcg ttagtgttgt tgcagcagct
     2581 ttttctggag gagcctctct agcactggca gctgttggtt tagctcttat ggttacggat
     2641 gctatagtac aagcagcgac cggcaattcc ttcatggaac aagccctgaa tccgatcatg
     2701 aaagcagtca ttgaaccctt aatcaaactc ctttcagatg catttacaaa aatgctcgaa
     2761 ggcttgggcg tcgactcgaa aaaagccaaa atgattggct ctattctggg ggcaatcgca
     2821 ggcgctcttg tcctagttgc agcagtcgtt ctcgtagcca ctgttggtaa acaggcagca
     2881 gcaaaacttg cagaaaatat tggcaaaata ataggtaaaa ccctcacaga ccttatacca
     2941 aagtttctca agaatttttc ttctcaactg gacgatttaa tcactaatgc tgttgccaga
     3001 ttaaataaat ttcttggtgc agcgggtgat gaagtaatat ccaaacaaat tatttccacc
     3061 catttaaacc aagcagtttt attaggagaa agtgttaact ctgccacaca agcgggagga
     3121 agtgtcgctt ctgctgtttt ccagaacagc gcgtcgacaa atctagcaga cctgacatta
     3181 tcgaaatatc aagttgaaca actgtcaaaa tatatcagtg aagcaataga aaaattcggc
     3241 caattgcagg aagtaattgc agatctatta gcctcaatgt ccaactctca ggctaataga
     3301 actgatgttg caaaagcaat tttgcaacaa actactgctt gatacaaata aggagaatgt
     3361 tatggaaatt caaaacacaa aaccaaccca gattttatat acagatatat ccacaaaaca
     3421 aactcaaagt tcttccgaaa cacaaaaatc acaaaattat cagcagattg cagcgcatat
     3481 tccacttaat gtcggtaaaa atcccgtatt aacaaccaca ttaaatgatg atcaactttt
     3541 aaagttatca gagcaggttc agcatgattc agaaatcatt gctcgcctta ctgacaaaaa
     3601 gatgaaagat ctttcagaga tgagtcacac ccttactcca gagaacactc tggatatttc
     3661 cagtctttct tctaatgctg tttctttaat tattagtgta gccgttctac tttctgctct
     3721 ccgcactgca gaaactaaat tgggctctca attgtcattg attgcgttcg atgctacaaa
     3781 atcagctgca gagaacattg ttcggcaagg cctggcagcc ctatcatcaa gcattactgg
     3841 agcagtcaca caagtaggta taacgggtat cggtgccaaa aaaacgcatt cagggattag
     3901 cgaccaaaaa ggagccttaa gaaagaacct tgccactgct caatctcttg aaaaagagct
     3961 tgcaggttct aaattagggt taaataaaca aatagataca aatatcacct caccacaaac
     4021 taactctagc acaaaatttt taggtaaaaa taaactggcg ccagataata tatccctgtc
     4081 aactgaacat aaaacttctc ttagttctcc cgatatttct ttgcaggata aaattgacac
     4141 ccagagaaga acttacgagc tcaataccct ttctgcgcag caaaaacaaa acattggccg
     4201 tgcaacaatg gaaacatcag ccgttgctgg taatatatcc acatcaggag ggcgttatgc
     4261 atctgctctt gaagaagaag aacaactaat cagtcaggcc agcagtaaac aagcagagga
     4321 agcatcccaa gtatctaaag aagcatccca agcgacaaat caattaatac aaaaattatt
     4381 gaatataatt gacagcatca accaatcaaa gaattcgaca gccagtcaga ttgctggtaa
     4441 cattcgagct taatatatcc aagagccata ataatatatg gctcttcctg taaggaaata
     4501 accatgaata taacaactct gactaatagt atttccacct catcattcag tccaaacaat
     4561 accaacggtt catcaaccga aacagttaat tctgatataa aaacaacgac cagttctcat
     4621 cctgtaagtt cccttactat gctcaacgac acccttcata atatcagaac aacaaatcag
     4681 gcattaaaga aagagctttc acaaaaaacg ttgactaaaa catcgctaga agaaatagca
     4741 ttacattcat ctcagattag catggatgta aataaatccg ctcaactatt agatattctt
     4801 tccaggcacg aatatccaat taataaagac gcaagagaat tattacattc agctccgaaa
     4861 gaagccgagc ttgatggaga tcaaatgata tctcatagag aactgtgggc taaaattgca
     4921 aactccatca atgatattaa tgaacagtat ctgaaagtat atgaacatgc cgttagttca
     4981 tatactcaaa tgtatcaaga ttttagcgct gttctttcca gtcttgccgg ctggatctct
     5041 cccggaggta acgacggaaa ctccgtgaaa ttacaagtca actcgcttaa aaaggcattg
     5101 gaagaactca aggaaaaata taaagataaa ccgctatatc cagcaaataa tactgttagt
     5161 caggaacaag caaataaatg gcttacagaa ttaggtggaa caatcggcaa ggtatctcaa
     5221 aaaaacgggg gatatgttgt cagtataaac atgaccccaa tagacaatat gttaaaaagc
     5281 ttagataatc taggtggaaa tggcgaggtt gtgctagata atgcaaaata tcaggcatgg
     5341 aatgccggat tctctgccga agatgaaaca atgaaaaata atcttcaaac tttagttcaa
     5401 aaatacagta atgccaatag tatttttgat aatttagtaa aggttttgag tagtacaata
     5461 agctcatgta cagatacaga taaacttttt ctccatttct gaggtgcgcc atgcataatg
     5521 taaataatac tcaagcgcca acattcttat ataaggcaac ttcaccatca tcaacagaat
     5581 acagcgagtt aaaaagcaaa atatccgata tccatagttc gcaaacttct ctaaaaacac
     5641 cagcatcagt gtctgaaaaa gaaaactttg caacgtcttt taatcagaaa tgtcttgatt
     5701 ttttattttc ttcctcaggg aaagaagatg tgttaagaag catttattcc aactcaatga
     5761 atgcgtatgc caaaagcgag attctcgaat tttcaaatgt tttgtactcc ttagtacatc
     5821 aaaatggtct taattttgaa aacgaaaagg gacttcaaaa aattgtcgca cagtattcgg
     5881 aactaattat aaaagataaa ttatcccaag attctgcctt tggaccatgg tcggcaaaga
     5941 ataagaaact ccatcaatta cgacaaaaca ttgagcacag acttgcacta ttagcacaac
     6001 aacacacatc tggtgaagct ttatcattgg gacaaaaact cctcaatact gaagtatcat
     6061 catttatcaa gaataatatt cttgctgaat taaagttaag taatgaaact gtttcatctc
     6121 tcaaactaga tgatttagtt gacgcacagg caaaacttgc ctttgatagt ttgcgcaatc
     6181 aacgtaaaaa tactattgat agtaaaggat ttggtatagg taaactgtca agagacttaa
     6241 atacagtagc cgtgtttcct gagctgttga gaaaagtcct taatgatatt ttagaagata
     6301 taaaagattc gcatcctatc caagatggcc tccctacacc tcccgaagat atgccagatg
     6361 gcggaccaac ccccggagcc aatgagaaaa catcccaacc tgtaattcac tatcatataa
     6421 ataatgataa tagaacttac gataatagag tttttgacaa cagagtatat gacaatagct
     6481 atcacgagaa cccagaaaat gatgcacagt ctcctacttc tcagacaaac gatctattat
     6541 cccgtaacgg aaactcatta ctaaatccac aaagagcact agttcaaaaa gtaacttccg
     6601 ttctaccaca ctctatatca gatactgtcc agacatttgc aaataattca gctttagaaa
     6661 aggctttcaa ccatactcca gataattcgg atggaatagg ttcagacctg ttaactacga
     6721 gtagtcaaga aagatctgca aataactctc tttctcgggg acacaggcct ctgaacatac
     6781 agaactcttc aaccaccccc cctctccacc cggaaggagt gacaagcagt aatgataact
     6841 catcagatac aactaaaagt agcgcttctc tttctcatag agtagcttcg caaatcaata
     6901 aattcaactc aaacactgat tcaaaagtac ttcagactga ttttttatca agaaatggag
     6961 acacatattt aacacgggaa acgatatttg aagcttcaaa aaaagtaaca aactccctaa
     7021 gtaatcttat atctctcatt ggaactaaat caggaacaca agaacgagag ttacaggaaa
     7081 aatcaaagga cattacaaaa tccacaacag aacatagaat aaacaacaaa ttaaaagtta
     7141 cagatgcaaa tataagaaac tacgtaacag aaaccaacgc agatacaatt gataaaaatc
     7201 atgcgatcta tgaaaaggca aaagaagtat ctagcgccct cagcaaggta ttgtcaaaaa
     7261 ttgacgatac ctctgcagaa ttacttacag atgatatatc tgatttaaaa aata
//
LOCUS       DAZSNG010000163         7188 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZSNG010000163 DAZSNG010000000
VERSION     DAZSNG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.166"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACHW4Z_003794"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACHW4Z_003794"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR8883242.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHW4Z_003795"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHW4Z_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR8883243.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHW4Z_003796"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHW4Z_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR8883244.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHW4Z_003797"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHW4Z_003797"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR8883245.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACHW4Z_003798"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACHW4Z_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHW4Z_003799"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHW4Z_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR8883246.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACHW4Z_003800"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACHW4Z_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR8883247.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAZSNG010000164         7183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZSNG010000164 DAZSNG010000000
VERSION     DAZSNG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.167"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(371..1198)
                     /locus_tag="ACHW4Z_003801"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHW4Z_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHW4Z_003802"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHW4Z_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR8883248.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHW4Z_003803"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHW4Z_003803"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR8883249.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHW4Z_003804"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHW4Z_003804"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR8883250.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHW4Z_003805"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHW4Z_003805"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR8883251.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHW4Z_003806"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHW4Z_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHW4Z_003807"
     CDS             7090..>7183
                     /locus_tag="ACHW4Z_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR8883252.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZSNG010000165         6848 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZSNG010000165 DAZSNG010000000
VERSION     DAZSNG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.169"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..236)
                     /locus_tag="ACHW4Z_003808"
     CDS             complement(<1..236)
                     /locus_tag="ACHW4Z_003808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883253.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACHW4Z_003809"
                     /pseudo
     CDS             complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACHW4Z_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACHW4Z_003810"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACHW4Z_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACHW4Z_003811"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACHW4Z_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR8883254.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(6734..>6848)
                     /locus_tag="ACHW4Z_003812"
     CDS             complement(6734..>6848)
                     /locus_tag="ACHW4Z_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883255.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
BASE COUNT         1651 a         1773 c         1784 g         1640 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgaaccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgt
     2761 atatactcgt ggcctttgcc ggaggagtca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcatt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct tcagatcgtg
     6661 aatctaaagg gttacatatt aactatattc tcaacaagac cagacactta tagaattagt
     6721 gatgatttat tcactaaaaa ggccatgaat tggaggaaaa cgatctgtcc agaaagtcta
     6781 tcggttcctc tcttattgcc tgattgtaat aatcgttatt atagtaagta ttgtttattt
     6841 catctaag
//
LOCUS       DAZSNG010000166         6795 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZSNG010000166 DAZSNG010000000
VERSION     DAZSNG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.170"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHW4Z_003813"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHW4Z_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR8883256.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHW4Z_003814"
     CDS             2739..4208
                     /locus_tag="ACHW4Z_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR8883257.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="ACHW4Z_003815"
     CDS             4208..5869
                     /locus_tag="ACHW4Z_003815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR8883258.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="ACHW4Z_003816"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="ACHW4Z_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR8883259.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="ACHW4Z_003817"
     CDS             complement(6478..>6795)
                     /locus_tag="ACHW4Z_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR8883260.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAZSNG010000167         6663 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZSNG010000167 DAZSNG010000000
VERSION     DAZSNG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.171"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..158)
                     /locus_tag="ACHW4Z_003818"
     CDS             complement(<1..158)
                     /locus_tag="ACHW4Z_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR8883261.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHW4Z_003819"
     CDS             complement(308..469)
                     /locus_tag="ACHW4Z_003819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883262.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHW4Z_003820"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHW4Z_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACHW4Z_003821"
     CDS             914..1249
                     /locus_tag="ACHW4Z_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR8883263.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACHW4Z_003822"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACHW4Z_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACHW4Z_003823"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHW4Z_003823"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACHW4Z_003824"
     tRNA            complement(2339..2414)
                     /locus_tag="ACHW4Z_003824"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACHW4Z_003825"
     CDS             complement(2559..3380)
                     /locus_tag="ACHW4Z_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR8883264.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACHW4Z_003826"
     CDS             complement(3395..3751)
                     /locus_tag="ACHW4Z_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8883265.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..4813)
                     /locus_tag="ACHW4Z_003827"
     CDS             complement(3764..4813)
                     /locus_tag="ACHW4Z_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8883266.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4815..5093)
                     /locus_tag="ACHW4Z_003828"
                     /pseudo
     CDS             complement(4815..5093)
                     /locus_tag="ACHW4Z_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5160..5411)
                     /gene="rem"
                     /locus_tag="ACHW4Z_003829"
     CDS             complement(5160..5411)
                     /gene="rem"
                     /locus_tag="ACHW4Z_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR8883267.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            complement(5629..5784)
                     /gene="hokD"
                     /locus_tag="ACHW4Z_003830"
     CDS             complement(5629..5784)
                     /gene="hokD"
                     /locus_tag="ACHW4Z_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8883268.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5856..6143)
                     /gene="relE"
                     /locus_tag="ACHW4Z_003831"
     CDS             complement(5856..6143)
                     /gene="relE"
                     /locus_tag="ACHW4Z_003831"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR8883269.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6143..6382)
                     /gene="relB"
                     /locus_tag="ACHW4Z_003832"
     CDS             complement(6143..6382)
                     /gene="relB"
                     /locus_tag="ACHW4Z_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR8883270.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6407..>6663
                     /gene="ydfV"
                     /locus_tag="ACHW4Z_003833"
     CDS             6407..>6663
                     /gene="ydfV"
                     /locus_tag="ACHW4Z_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR8883271.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
BASE COUNT         1772 a         1782 c         1398 g         1711 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgta
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgcttca gtatgctggt ggcggtctgc tctcccggca
     4201 cgatgtcgct ttcacggtac attgagcgga ttttttccgc acgcaacccc agcgaacgac
     4261 gtaataccgc ttccggtagc gcgtccgcca cctgattgcg gaccgcccac caggataatt
     4321 cagccagaga taattcacgc tcctgcgtac cgcttattgc gtgaccgatg acgtcaatca
     4381 tccatgctga caggttttga tgagcaagtt gctcgagtga ttcggatgtc tggtcacgca
     4441 gctggttgtc gcagtgccag cacaatacca ttgcgccggt accataacgg tgaatgacgg
     4501 tttcgctgtg atgataatcg ccgtgtggcc actggcagga tttaatatgg cgcaacagcc
     4561 agtcagacaa tgcgccagca ccaccagcag cacgaatcac ccgtgcgtta ctgaaaaacg
     4621 gcagcaatgt tttgtcttcc actagcggct ggcgaacggc aggaacgacc ccggacggca
     4681 gattacgcat gcttttcggt tccggctcca ccagtacccg ggtattgtgg aataccggca
     4741 tggattcacg accgggctta aggaccacca gcccgagttc cggaaccaga acaggtcgaa
     4801 gtaatacccg cacgttacct ccagatccgt tgctggaatg tgcgggacgg acgcggtggc
     4861 tgttcggagt aagggagcct gacggagatt atctagtgac ggtagtcgag gctaagggct
     4921 tttttaacct cgcatccgcg cctgcggtaa cactgaataa gccattcggc ctgttcttca
     4981 gtgcatgagg gatgctggta ccagtctgac ttaaatgcgt gagaataccg ctcgtgcgtg
     5041 tgggcaagaa cggtcgaatt atcatgattg taatattttg cgttgcgtgc catcggtttt
     5101 ctccggtggc acggtgttac tcagcgggag ttcagccccg cgcaagattg tagatgagtt
     5161 tattcttctg aaaaagcaga aaagccagct tttattccga tctctttcaa tgcctgtaat
     5221 gaagtgacaa actcaccgtc gcgcaagata aatccgtccg tcactcgggc atccacaaaa
     5281 ttaattaacg cagccccatt tttttgcaaa cacacaatgc ggtaatgact aacaatattt
     5341 ccattttcaa cgcacacagc atagaggcca tcttcacaaa aaattttacg cagttcttcg
     5401 atgttcatca tcagaatcct tccagataag tagcactccc ctgttcgggg tccatccctt
     5461 ttatccctgc gcgctactta agtatttttg attctattcc ggcaccatcc agaacttcaa
     5521 atgcgttgaa aataaaaaca aaaaaccgcc gaagcgggtt aagtgcgggt gcgttgagga
     5581 tgcctgccac atcagaggtg gcgagggatt tctcactcgc cgggtctctt actcctcagg
     5641 ttcgtaagct gtgaagacag cgacctccgt ctggccggtt cggattcgta cctcgcagag
     5701 gtctttcctc gttaccagtg ccgtcactat gacggttaaa cagatgacga tcagggcgat
     5761 taacatcgcc ttttgctgct tcatagcctg cttctcctgt caacgcaaag cagaagtgtc
     5821 accttcggtg cgaaacagag atgtcatgct ttggttcaga gaatgcgttt gaccgcctcg
     5881 ctatatactt ccgagcgttc tcttttccca acagaaatca cgaaaacgac aactttctcg
     5941 tctataacct ggtatacaag gcgatagcct gaagaccgga gcttaatctt gtaacaatca
     6001 ggcataccac ggagcttgtt tgcttcaatc cggggtgact caagtacttc aaccagcttc
     6061 tttttcaact gttcacgtac cgtcgagccc agctttcgcc attcctttag tgcccgctcg
     6121 tcaaaatcca gaaaatacgc catcagagtt catccagcgt cacacgtact ggcttaggat
     6181 tacgaagccg ttctttcact atctccacaa gttcagcatc ttcatcactc aggagtgtct
     6241 gtttgaacgg caagcgttca ttgtcagcga tatactcgag catgagacga agcgcttcag
     6301 aaggagttac acccattttt tcaagcgcgg cgtaagaacg cgctttaagt tcatcgtcaa
     6361 tacgcaggtt aatgctaccc atgtcttaca cctcttgtaa ttacaaatgt cattacaagt
     6421 atcgcactac aacatgctta gggcaagtca cgaaggaagt cagaaagtag tcgtaagaac
     6481 ggtgatcact gtccgctttg tgccaggagc agccattgct aagtccatcc tgtattgtgc
     6541 aggtcagctc ttttttaaag agtccggcca tcatcttact ggtacagaca ccatatactt
     6601 tgtgacggtc aggctacata tgcacaactc aacttattca tctatttttt gctttagcat
     6661 gtc
//
LOCUS       DAZSNG010000168         6384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZSNG010000168 DAZSNG010000000
VERSION     DAZSNG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.172"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHW4Z_003834"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHW4Z_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8883272.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHW4Z_003835"
     CDS             2219..2767
                     /locus_tag="ACHW4Z_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR8883273.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHW4Z_003836"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHW4Z_003836"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR8883274.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHW4Z_003837"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHW4Z_003837"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR8883275.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHW4Z_003838"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHW4Z_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR8883276.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1618 a         1559 c         1617 g         1590 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatg
//
LOCUS       DAZSNG010000169         6321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZSNG010000169 DAZSNG010000000
VERSION     DAZSNG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.173"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..378
                     /locus_tag="ACHW4Z_003839"
     CDS             <1..378
                     /locus_tag="ACHW4Z_003839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883277.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHW4Z_003840"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHW4Z_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR8883278.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHW4Z_003841"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHW4Z_003841"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR8883279.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEEL"
     gene            complement(1787..2476)
                     /locus_tag="ACHW4Z_003842"
     CDS             complement(1787..2476)
                     /locus_tag="ACHW4Z_003842"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR8883280.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHW4Z_003843"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHW4Z_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR8883281.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPM
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHW4Z_003844"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHW4Z_003844"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR8883282.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHW4Z_003845"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHW4Z_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR8883283.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1611 a         1710 c         1563 g         1437 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa ttcttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccatt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAZSNG010000170         6259 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZSNG010000170 DAZSNG010000000
VERSION     DAZSNG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.174"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHW4Z_003846"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHW4Z_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR8883284.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHW4Z_003847"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHW4Z_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR8883285.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHW4Z_003848"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHW4Z_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACHW4Z_003849"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACHW4Z_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1663 a         1455 c         1471 g         1670 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta tatattttta
     6181 ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
     6241 cgaagtaaag ggagtgaat
//
LOCUS       DAZSNG010000171         6250 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZSNG010000171 DAZSNG010000000
VERSION     DAZSNG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.175"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="ACHW4Z_003850"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="ACHW4Z_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR8883286.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="ACHW4Z_003851"
     CDS             578..1027
                     /locus_tag="ACHW4Z_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8883287.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /gene="ydcY"
                     /locus_tag="ACHW4Z_003852"
     CDS             complement(1028..1261)
                     /gene="ydcY"
                     /locus_tag="ACHW4Z_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR8883288.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="ACHW4Z_003853"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="ACHW4Z_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR8883289.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="ACHW4Z_003854"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="ACHW4Z_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR8883290.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="ACHW4Z_003855"
     CDS             complement(2212..3021)
                     /locus_tag="ACHW4Z_003855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR8883291.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="ACHW4Z_003856"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="ACHW4Z_003856"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR8883292.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="ACHW4Z_003857"
     CDS             complement(4677..5471)
                     /locus_tag="ACHW4Z_003857"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8883293.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="ACHW4Z_003858"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="ACHW4Z_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5836..6081
                     /locus_tag="ACHW4Z_003859"
                     /pseudo
     CDS             5836..6081
                     /locus_tag="ACHW4Z_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1636 a         1574 c         1420 g         1620 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cggggctgaa tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg
     6061 ctgaggtata tgccggcctg acacaggcta agtgtgcccg ggaatcggtg caagtataaa
     6121 aaacagcctg ttgcggtata agaatcgacc atgcagagag agaaaacacc ggagtggagg
     6181 gaaaagcaga aaagctcacg tggcataagg cgtggacagc ggtatcgtct ggtttttcaa
     6241 tttcctatac
//
LOCUS       DAZSNG010000172         6058 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZSNG010000172 DAZSNG010000000
VERSION     DAZSNG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.176"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..143)
                     /locus_tag="ACHW4Z_003860"
     CDS             complement(<1..143)
                     /locus_tag="ACHW4Z_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR8883294.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHW4Z_003861"
     CDS             379..651
                     /locus_tag="ACHW4Z_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR8883295.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHW4Z_003862"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHW4Z_003862"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR8883296.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="ACHW4Z_003863"
     CDS             1111..1731
                     /locus_tag="ACHW4Z_003863"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8883297.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACHW4Z_003864"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACHW4Z_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="ACHW4Z_003865"
     CDS             complement(3114..3767)
                     /locus_tag="ACHW4Z_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8883298.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="ACHW4Z_003866"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="ACHW4Z_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="ACHW4Z_003867"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="ACHW4Z_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAZSNG010000173         6052 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZSNG010000173 DAZSNG010000000
VERSION     DAZSNG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.177"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            430..1338
                     /locus_tag="ACHW4Z_003868"
     CDS             430..1338
                     /locus_tag="ACHW4Z_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR8883299.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACHW4Z_003869"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACHW4Z_003869"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8883300.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="ACHW4Z_003870"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="ACHW4Z_003870"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR8883301.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACHW4Z_003871"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACHW4Z_003871"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR8883302.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="ACHW4Z_003872"
     CDS             complement(5673..>6052)
                     /locus_tag="ACHW4Z_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883303.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAZSNG010000174         5890 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZSNG010000174 DAZSNG010000000
VERSION     DAZSNG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.178"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..244
                     /locus_tag="ACHW4Z_003873"
     CDS             <1..244
                     /locus_tag="ACHW4Z_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883304.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHW4Z_003874"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHW4Z_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHW4Z_003875"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHW4Z_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR8883305.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHW4Z_003876"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHW4Z_003876"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR8883306.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHW4Z_003877"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHW4Z_003877"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR8883307.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHW4Z_003878"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHW4Z_003878"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR8883308.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="ACHW4Z_003879"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="ACHW4Z_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1455 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaac
//
LOCUS       DAZSNG010000175         5744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZSNG010000175 DAZSNG010000000
VERSION     DAZSNG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.179"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..173
                     /locus_tag="ACHW4Z_003880"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHW4Z_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHW4Z_003881"
     CDS             complement(489..827)
                     /locus_tag="ACHW4Z_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883309.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHW4Z_003882"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHW4Z_003882"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8883310.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHW4Z_003883"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHW4Z_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHW4Z_003884"
     CDS             complement(2165..2650)
                     /locus_tag="ACHW4Z_003884"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8883311.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHW4Z_003885"
     CDS             complement(2906..3886)
                     /locus_tag="ACHW4Z_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR8883312.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHW4Z_003886"
     CDS             complement(3886..5085)
                     /locus_tag="ACHW4Z_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8883313.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHW4Z_003887"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHW4Z_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHW4Z_003888"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHW4Z_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8883314.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZSNG010000176         5738 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZSNG010000176 DAZSNG010000000
VERSION     DAZSNG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.180"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..241
                     /locus_tag="ACHW4Z_003889"
     CDS             <1..241
                     /locus_tag="ACHW4Z_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883315.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHW4Z_003890"
     CDS             complement(238..1179)
                     /locus_tag="ACHW4Z_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR8883316.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHW4Z_003891"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHW4Z_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHW4Z_003892"
     CDS             complement(1684..2904)
                     /locus_tag="ACHW4Z_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR8883317.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHW4Z_003893"
     CDS             complement(2908..3651)
                     /locus_tag="ACHW4Z_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR8883318.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHW4Z_003894"
     CDS             complement(3542..5008)
                     /locus_tag="ACHW4Z_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR8883319.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHW4Z_003895"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHW4Z_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1434 g         1460 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc catcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZSNG010000177         5702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZSNG010000177 DAZSNG010000000
VERSION     DAZSNG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.181"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..170)
                     /locus_tag="ACHW4Z_003896"
     CDS             complement(<1..170)
                     /locus_tag="ACHW4Z_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883320.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHW4Z_003897"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHW4Z_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR8883321.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHW4Z_003898"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHW4Z_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHW4Z_003899"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHW4Z_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR8883322.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHW4Z_003900"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHW4Z_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHW4Z_003901"
     CDS             5484..>5702
                     /locus_tag="ACHW4Z_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR8883323.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZSNG010000178         5600 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZSNG010000178 DAZSNG010000000
VERSION     DAZSNG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.182"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..410
                     /locus_tag="ACHW4Z_003902"
     CDS             <1..410
                     /locus_tag="ACHW4Z_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8883324.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="ACHW4Z_003903"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="ACHW4Z_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACHW4Z_003904"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACHW4Z_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR8883325.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatcg
//
LOCUS       DAZSNG010000179         5590 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZSNG010000179 DAZSNG010000000
VERSION     DAZSNG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.183"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..279)
                     /locus_tag="ACHW4Z_003905"
     CDS             complement(1..279)
                     /locus_tag="ACHW4Z_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883326.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHW4Z_003906"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHW4Z_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8883327.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHW4Z_003907"
     CDS             complement(1254..1670)
                     /locus_tag="ACHW4Z_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883328.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHW4Z_003908"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHW4Z_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHW4Z_003909"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHW4Z_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHW4Z_003910"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHW4Z_003910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHW4Z_003911"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHW4Z_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR8883329.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHW4Z_003912"
     tRNA            3996..4071
                     /locus_tag="ACHW4Z_003912"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHW4Z_003913"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHW4Z_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZSNG010000180         5577 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZSNG010000180 DAZSNG010000000
VERSION     DAZSNG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.184"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..248)
                     /locus_tag="ACHW4Z_003914"
     CDS             complement(<1..248)
                     /locus_tag="ACHW4Z_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883330.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="ACHW4Z_003915"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="ACHW4Z_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="ACHW4Z_003916"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="ACHW4Z_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="ACHW4Z_003917"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="ACHW4Z_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHW4Z_003918"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHW4Z_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR8883331.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="ACHW4Z_003919"
     CDS             complement(2964..3278)
                     /locus_tag="ACHW4Z_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883332.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="ACHW4Z_003920"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="ACHW4Z_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHW4Z_003921"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHW4Z_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR8883333.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="ACHW4Z_003922"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="ACHW4Z_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="ACHW4Z_003923"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="ACHW4Z_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1508 a         1222 c         1131 g         1716 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaa
//
LOCUS       DAZSNG010000181         5557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZSNG010000181 DAZSNG010000000
VERSION     DAZSNG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.185"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..641
                     /locus_tag="ACHW4Z_003924"
     CDS             <1..641
                     /locus_tag="ACHW4Z_003924"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR8883334.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHW4Z_003925"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHW4Z_003925"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR8883335.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHW4Z_003926"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHW4Z_003926"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR8883336.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZSNG010000182         5502 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZSNG010000182 DAZSNG010000000
VERSION     DAZSNG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.186"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(109..711)
                     /gene="fimB"
                     /locus_tag="ACHW4Z_003927"
                     /pseudo
     CDS             complement(109..711)
                     /gene="fimB"
                     /locus_tag="ACHW4Z_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            2165..2881
                     /gene="nanC"
                     /locus_tag="ACHW4Z_003928"
     CDS             2165..2881
                     /gene="nanC"
                     /locus_tag="ACHW4Z_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR8883337.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2901..4007
                     /gene="nanM"
                     /locus_tag="ACHW4Z_003929"
     CDS             2901..4007
                     /gene="nanM"
                     /locus_tag="ACHW4Z_003929"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR8883338.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4072..5052
                     /locus_tag="ACHW4Z_003930"
     CDS             4072..5052
                     /locus_tag="ACHW4Z_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR8883339.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCRMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            5162..5367
                     /locus_tag="ACHW4Z_003931"
                     /pseudo
     CDS             5162..5367
                     /locus_tag="ACHW4Z_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1672 a         1144 c         1158 g         1528 t
ORIGIN      
        1 attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt ttgatatatc
       61 gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc
      121 atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc
      181 ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt
      241 atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat
      301 ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg
      361 agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga
      421 agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt
      481 tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc
      541 cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat
      601 cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat
      661 ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc
      721 ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct
      781 gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct
      841 gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat
      901 gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc
      961 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     1021 aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga
     1081 cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt
     1141 agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag
     1201 cttaggccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga
     1261 cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc
     1321 tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     1381 aacaccaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca
     1441 aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta
     1501 ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat
     1561 tatttataaa acgtatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt
     1621 cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa
     1681 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     1741 agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc
     1801 agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga
     1861 tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta
     1921 cgaatattca aatcaatgaa aaatcagaag agattttata ctcaggtatt taatctggat
     1981 ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata
     2041 taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac
     2101 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
     2161 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
     2221 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
     2281 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
     2341 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
     2401 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
     2461 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
     2521 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
     2581 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     2641 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga
     2701 ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca
     2761 tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc agggtgttta
     2821 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     2881 gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt
     2941 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     3001 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca
     3061 agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac
     3121 cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg
     3181 gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     3241 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt
     3301 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     3361 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc
     3421 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     3481 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     3541 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     3601 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     3661 atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     3721 ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     3781 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     3841 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     3901 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     3961 cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     4021 cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca
     4081 ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     4141 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa
     4201 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt
     4261 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     4321 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
     4381 ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg
     4441 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     4501 tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca
     4561 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgcc ggatgcaagg cgaatttgac
     4621 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     4681 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
     4741 tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat
     4801 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     4861 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     4921 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     4981 acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg
     5041 cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga
     5101 caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc
     5161 aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt
     5221 attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg
     5281 gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac
     5341 cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat
     5401 gatatgctta atgatgttca aagaaattag gccattatta tattattgct tgtattttct
     5461 ttcattccta aaagcacccg ccattaatcc caggcgcatt at
//
LOCUS       DAZSNG010000183         5487 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZSNG010000183 DAZSNG010000000
VERSION     DAZSNG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.187"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1632)
                     /locus_tag="ACHW4Z_003932"
     CDS             complement(<1..1632)
                     /locus_tag="ACHW4Z_003932"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR8883340.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWV"
     gene            complement(1648..2543)
                     /locus_tag="ACHW4Z_003933"
                     /pseudo
     CDS             complement(1648..2543)
                     /locus_tag="ACHW4Z_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2543..3148)
                     /locus_tag="ACHW4Z_003934"
     CDS             complement(2543..3148)
                     /locus_tag="ACHW4Z_003934"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8883341.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3319..>5487)
                     /locus_tag="ACHW4Z_003935"
     CDS             complement(3319..>5487)
                     /locus_tag="ACHW4Z_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR8883342.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1364 c         1141 g         1400 t
ORIGIN      
        1 tacccacggt ttaccatctt tatgactggt taacaccccg gcaatcagct ccagttgtgg
       61 gtgccacggc tgcccggtgt agatgagaat gccacccggt tcgatggcat tggctagacc
      121 tgcgagtgag ttttttacct gctcattttc gggaaaaagc tcatataggc cagagacaat
      181 cgccagcgta ggccgcggag ttaacgcgct gagttcctcc gggttaaacg catcgccctg
      241 ttcaaaacgc acccgcccag acattccccg ttgggcaatc atctcttgcc cctgtgcaac
      301 atttaactca ctgtaatcac gtaacaaaat atcgcttacg gcaggctcgt ttgccagcgc
      361 atccagtaca tagcgtccat gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt
      421 ggcgtggaga tcggcaacgg cttgtttaat caatatttgc aaatgggttt tgcgctggcg
      481 aataccgcgc catccaacac tgttcaggta gattttgtcg attaaacgcc cgaatgcgtt
      541 actgccttgc ggttgatttt gatagacata atccagggaa ctgcctgaat caaagccggt
      601 actcattccg agatgcaggc ccgcagagtg cgcaccgaac aatttcatcg cctttcgcat
      661 aaagcgatac gctaaatcaa ccggcgataa tggcacgggt ccaccagaaa ggagccgcca
      721 tcgatccgct gatggtcctg tgcggtcttc atgctgataa tcaaattttt gcgatttgtt
      781 agcatataag cggcagataa agctttgcat tttttcaaat gcctgcgccc tgttctcttc
      841 acccaatgtg tcgtgataaa agcctggcag cagatgcagc tctttcagag ggctacgtaa
      901 tctctgataa aaatcaatct gcggttggcg atgcaccaca tagtcatcac ctgatatcag
      961 aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca atacgttcag acgttttata
     1021 gagatcgagc aagatattga cggcaatcgc ccgtgtgatc agcggatcat tattgaaact
     1081 cgccacccgt tgccgatcgt gggtcaaata gcgccctttc acataggaat taataaaaaa
     1141 tagaccacgc agacgatgcc ataacgccag cgcaggacgt gccagcggca catacaattt
     1201 aaccttaaag gccggagaag ccagcaccag cccacgaatt gcaggtgcat aatcatgaac
     1261 ccatgtggca acaagcactg cgccgacgct ttgcgcgatc actaccacct cttccagtcc
     1321 gacctggctg tcgctggcag caaaacggac aaattcatcg acatcctgca ctgagcgcat
     1381 aagagacggg ctataaccac gcggccccga attttttcca tgcccccggg catcccaggc
     1441 ataaaaagca gtatcaggca tcgccagttc atcaacgaga tgttgtagac gaccagaatg
     1501 ttcatgcccg cgatgaaata agacgatgac ctttttcgcc cctggctgta aagtgggcca
     1561 gtgccgataa tacaatgctg tattatcact ggtggtaaaa aaatgttccc cagggatgcg
     1621 tgaattttcc attttctcta ttttccttca gtaacagcag tagcgaataa aataaaaaaa
     1681 taccggagcg gtgaaaatta atgagtcaat cctgtcaagg agtccgccat gtcctagtaa
     1741 tagttttcca ctatctttaa caccaatatc tcgtttgatg gctgacatca ctacatcgcc
     1801 gcaaaaacca ctaataccaa ttaacaaacc cgctaataat gcctgtaatg tatttagcgg
     1861 tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg gtgattacgc cccccagcag
     1921 accttccagc gtttttcccg ggctgacttt ggggaccact tttcttctgc cgcaggattt
     1981 cccccataaa tactgtgcaa tatcgtttga ctcggttaac gccaaaagaa ataacaccag
     2041 taacgccccg ccctggatat ttatggttgg caacattaat agccaggcgg catgactcaa
     2101 ggcaaaaacg gtcattatcc atccccaaaa aatggcgctc acggtatgct gaaaaccgga
     2161 ggggtcaccc acaaaaactc gccaggtggc taatatcaga aacccagtca aaggtataaa
     2221 taacagaaac agctcaaagc aattaaaacc gataagcaaa tagttaagag gaataaccca
     2281 atataaccaa cgcgaaaaat gtacggatat aagcgtacaa tattctttca atgccagaaa
     2341 acttatcagc gtaaaaaacg tcaacgtcat ccagcgtggg cctgaaatca ccagtgaaaa
     2401 acaaatgacg atgccccacc aggtacgaat acgtaatgta acttcatccc cttttctctc
     2461 aggtaaccgc catagcagaa aacggtttat cagcttgcta aaattaacaa cgcgaaaagt
     2521 gttgccagag atttttccag cattatcttt cagcgctcat aagaagcaca ctccgacaac
     2581 gattaatcgc agtacagata agcagaattg aagcaatgct ccataaaaga ttattccatt
     2641 gcatccactg tggataaatg gcaacggcca gaccccacag accaaatatt aacgcgcgat
     2701 cacttttgcc aaatggaccg gcataactgc gaacaccgtt aatcgtctgc gcgagtaaac
     2761 cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat cacgagtgat gcgttacttt
     2821 ccggtaaaaa taaaaacggt aaatagagcg caatatcgga aataacatcg ccagtctcat
     2881 tcaaaatcgc ccccagacgt gtttgctgat tgcactcacg cgccaacatg ccatccagcg
     2941 cattgagcgc catacggata aaaagcacga tgggcaatag caaaaagagg atgggttgtg
     3001 ccaccaacac caacagcaat ccggtaaaaa gagaaagcgc cagtgccgta agagtgatgt
     3061 gattcgctgt aacgtggtgc ttataaagcc aaaacatcgt cggccttaac agcgactgaa
     3121 agagcggttt tatctggtag agcgtcatta tcaatccttg ataattcggt ttgcgctatg
     3181 gtgcatcaat tcccctgacg gagatatcgc aacacgccgc ttcattaaaa attcagaata
     3241 tatcaaggta ttgggggctc actgtcagga atgattatcc ccaataaatc aagtagttaa
     3301 aaattattac gctgttattc aatacaactc atcaagtttc ctcatcgctt gttcccgtat
     3361 tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa tccagttcag ggttaacaag
     3421 ctctaacgcc agcaaaggat cacgtttcag cctcgccttc aatacatcaa ctggaaagcg
     3481 gggattattc atcgccacaa tccaccactc gggatcaatt tctgccgcca gtgccgcaga
     3541 gggggtgtgg cggttgctaa gtgcgccgaa cttgaaaaat gtgagattgc ttttcgccag
     3601 tgtactccac catatacgat ccggttgttt tgccagtaca tccagttgtg ctaacgcatc
     3661 ggggtcgagt gcataataaa cacgtaatgc ttcatcaacc tgcgcacggg agctattttc
     3721 ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc gtgttttgct gtaattcctt
     3781 gagaacaaca gcgggtaaat tatcccaact aactaatgca ctctctaaaa agtcccagtc
     3841 ttccaaacgt atcagggcgg cggcatttaa tagcatctgg tcatcaactt cgccgtggct
     3901 aatcagctca gacaaaatca tatcagcgct ggtttcatat gcatctgaat ctgctttcgg
     3961 attatttttc cgatgcgata aagcacgttg ccaaattttt tgtttcatcg acgtgctcag
     4021 atactgattt ttcgccagat tactccaccc ctcaggatcg ttgaaacgtg tcaataaccg
     4081 ttgtaaagca gcgtcagagt gaattacctg catatcccga cagcaaaagt aggcagggga
     4141 ttttttctcc atccttttaa caaaattaaa ctgtaagtta tctggcaatg ctaaaaataa
     4201 cacacgtatt agatcatcgt cattctcgaa tttttggtat aaataggctg caactttgtc
     4261 actctcttgt ggcgtcatcc gcaataattg accatggtgt ttcatctctg ccagcttgaa
     4321 cgctaagctt gtgacaacat tgtttgatga atcgttcgcc aactgcattg cctgattgta
     4381 agtgagttgt cctccagcac cgacctgacg aatagaggaa tcatcactgc ggatatagcc
     4441 atcaataatg gattcgggca ataaccacgc atctttataa agctcctgaa tgatcatatc
     4501 ccaatctctt ttcgccagag tcatgacgat atccagaggg agattttcac gtagcgcaag
     4561 attcaacgga ccgacaccat caggtagacg actcattcgc gtcattattt cgacagagag
     4621 cggttcacgg tcgagcaacc ccagaacaac aggctggtaa cccaattgct gatcggtcaa
     4681 ctccagaatc tcattgtcag gcatatatct cgccatactt tcaatcgttt gttcatcatt
     4741 gctattaata agttgtttgc gtaggttagc tgaaattcct ttaagccttg ggagagttcg
     4801 cattatggca agatcaaatg tcgcaatatc tcgtccttca aatgcagcaa cgttgccttc
     4861 atccagcatt tgcaattcct gcactaactc aggagaaggc ttagggcgaa acagcgcttc
     4921 ttttttcatc tgattacaga cattccctgc catgcgaata ttgcttcgct taaaaatttc
     4981 tttcagagca tcttcatcat ttattagacc ccaataatat gcatcaggat ttattatcat
     5041 cagcgctttt ttgacatttt cagtgtcatt tttgtcactc gcacggacca tgaattcact
     5101 atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg ataaataccc cacgcgcatt
     5161 aatactcccc ttaatcgtag acggattatc gtaatatgag tcgataaata ccagtccact
     5221 tgcatttatc gaaccggtaa ttaacagtgg tgaatccgta actatattcg ttgccgtcac
     5281 gttaccgaga ataacccatg gattagaaag agagaaagtg gaaatattac ctttgactgt
     5341 caaattgcca agaacaatca atttttgatt actctctata ttaccttcta ccactaaatc
     5401 gccacgatgc actttccagt ccgatgcaga taaaccgaag gggacaagat tcacgctact
     5461 gttgatagct tgttcaactt gttgttt
//
LOCUS       DAZSNG010000184         5399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZSNG010000184 DAZSNG010000000
VERSION     DAZSNG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.188"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..497
                     /locus_tag="ACHW4Z_003936"
     CDS             <1..497
                     /locus_tag="ACHW4Z_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR8883343.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHW4Z_003937"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHW4Z_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHW4Z_003938"
     CDS             2500..3114
                     /locus_tag="ACHW4Z_003938"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR8883344.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHW4Z_003939"
     CDS             3114..3443
                     /locus_tag="ACHW4Z_003939"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR8883345.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHW4Z_003940"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHW4Z_003940"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR8883346.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHW4Z_003941"
     CDS             4529..4840
                     /locus_tag="ACHW4Z_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8883347.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHW4Z_003942"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHW4Z_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1178 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaa
//
LOCUS       DAZSNG010000185         5370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZSNG010000185 DAZSNG010000000
VERSION     DAZSNG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.189"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            355..876
                     /locus_tag="ACHW4Z_003943"
     CDS             355..876
                     /locus_tag="ACHW4Z_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR8883348.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            932..1165
                     /locus_tag="ACHW4Z_003944"
     CDS             932..1165
                     /locus_tag="ACHW4Z_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR8883349.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1229..3187
                     /locus_tag="ACHW4Z_003945"
     CDS             1229..3187
                     /locus_tag="ACHW4Z_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR8883350.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3242..3676
                     /gene="psiB"
                     /locus_tag="ACHW4Z_003946"
     CDS             3242..3676
                     /gene="psiB"
                     /locus_tag="ACHW4Z_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR8883351.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3673..4435
                     /locus_tag="ACHW4Z_003947"
                     /pseudo
     CDS             3673..4435
                     /locus_tag="ACHW4Z_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            4614..4763
                     /gene="mok"
                     /locus_tag="ACHW4Z_003948"
                     /pseudo
     CDS             4614..4763
                     /gene="mok"
                     /locus_tag="ACHW4Z_003948"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4705..4830
                     /locus_tag="ACHW4Z_003949"
     CDS             4705..4830
                     /locus_tag="ACHW4Z_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR8883352.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(5149..5370)
                     /locus_tag="ACHW4Z_003950"
                     /pseudo
     CDS             complement(5149..5370)
                     /locus_tag="ACHW4Z_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1208 a         1430 c         1661 g         1071 t
ORIGIN      
        1 aaccgcgctt cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac
       61 ctgtttaagc ccctgtcgcc gggggcatag acagccacaa cctgccgcac ctgtccaggg
      121 taaatgaaca gcccacaaaa ttttttaccc aaaacccggt aaaaaatttt gcgttctgcc
      181 caggacaggt gcgtcaggcc gtggcagtga tgccccatgc ggcgaaggtt ctcaaacagg
      241 tgatggtgaa taccggaggg aacaaagagc aggtccccgc aaaacgtcaa aaccgcggct
      301 cccgaaactc aacagatgat gggggctgaa aacatcaaca gaaggagaca catcatggca
      361 gttcgtggca ttaacaaggt cattcttgtc ggtcgtctgg gaaaagatcc ggaagtccgt
      421 tacatcccca acgggggcgc agtggcaaac ctgcaggtgg ccacgtcaga aagctggcgt
      481 gacaaacaga cgggggagat gcgggagcag acagaatggc atcgcgtggt gctgttcggc
      541 aagctcgcgg aagtggcagg tgaatatctg cgcaagggcg cgcaggtcta catcgagggg
      601 caactccgaa cccgtagctg ggaagataaa ggtattaccc gttacgtcac tgaaatcctt
      661 gtgaaaacca cgggcaccat gcagatgctg gggagtgcac cacagcagaa cgctcaggcg
      721 caaccgcagc ctcagcagaa tggtcagcca cagagtgctg acacgacgaa aaaagggagc
      781 gcgaaaacaa aaggccgtgg acgtaaggcc gcgcagccgg agcctcagca gccgtctgaa
      841 cccgcgtatg acttcgatga cgacattccg ttctaacgaa agacagtgac aaccgccccg
      901 tccagtgcgg ggcatcaccg gagatatgag gatgagcgaa tatttcagaa tatttcaggg
      961 gctgcctgac ggctccttta cccgcgaaca ggctgaagcc gttgccgcac agtaccggaa
     1021 tgtctttatc gaggatgatc agggaacgca ttttcgcctg gttgtccgca ataacagtgg
     1081 cctggtctgg cgtacctgga attttgagga cggtgccggg tactggatga accatgtcat
     1141 ccggaatttc ggcattctta agtaagagag gtgccggacg ggaacgcgtc cggcagacat
     1201 acaaccatcc cggtcaaagg agaaatatat gtcagttaca gagtctaaga caaaaacgga
     1261 gcgtaaatcc agccgtaaac ctgcaaaaac gcaggaaaca gtcctgtctg ccctgctgga
     1321 gcagacggag gaagtgagcg tgtcgctggc ctcgctgatt aaatcaccgc tgaatgtgcg
     1381 cacggtgccg tattccgcag agtccgtcag cgaactggcg gagtccatta agggcgttgg
     1441 cctgttgcag aatctggttg ttcatgccct gcctggtgac cgccacggtg tcgccgcagg
     1501 tggtcgccga ctggcagcac tcaacatgct ggcagagcgt ggcatccttc cggctgactg
     1561 gcctgtacgc gtgaaggtta ttccgcagga gctggcgacg gctgcatcaa tgactgaaaa
     1621 cggtcagcgt cgggatatgc accctgccga acagattgcc ggattccgcg caatggcgca
     1681 ggaaggcaaa acacctgcac aaatcggtga tttgctgggc tattcacccc gccacgttca
     1741 gcgaatgctg aaactggctg accttgcgcc tgtcatcctc gatgcgctgg cagaagaccg
     1801 catcaccacc gaacactgtc aggcgctggc gctggagaac gacaccgcgc gtcaggtgca
     1861 ggtgtttgaa gccgcctgtc agtcgggatg gggcggtaaa ccggaagtac agaccattcg
     1921 tcgtctggtg accgaaagtg aagtggcggt ggcggggaac agtaaattcc gcttcgtggg
     1981 ggctgatgcc ttctcgccag acgaactgcg caccgatttg ttcagcgacg acgaaggtgg
     2041 ctatgtggac tgcgtggcgc tcgatgccgc cctgctggaa aaactccagg ctgtcgcaga
     2101 gttccttcgg gaagccgaag gctgggaatg gtgcgccgga cgcatggagc ctgtcggtga
     2161 gtgccgtgag gatgccggaa cataccgctg tctgccggag ccggaagcgg tgctgacgga
     2221 ggcggaagaa gaacgtctga acgaactgat gacgcgttac gacgcgctgg aaaaccagtg
     2281 tgaggaatct gacctgctgg aagcagaaat gaagctgatg cgctgcatgg cgaaggtcag
     2341 ggcgtggacg ccggagatgc gtgccggagg tggagtggtg gtgtcctggc gttatggcaa
     2401 cgtgtgtgtc cagcgtggtg tgcagttgcg cagtgaggat gacgtggctg acgacgctga
     2461 ccgtacggaa caggtgcagg agaaagcgtc agtggaggaa atcagtctgc cactgctgac
     2521 gaaaatgtct tccgaacgca cgctggcagt ccaggcagca ctgatgcagc aaccggacaa
     2581 atccctgaca ctgctggcat ggacgctctg cctgaatgtg tttggcagcg gagcgtacag
     2641 taaaccagca caaatcagcc tggaatgtaa acattattcg ctgaccagcg atgcgccatc
     2701 ggggaaggaa ggtgccgcat tcatggcgct gatggcagaa aaagcccgtc ttgctgccct
     2761 gttaccggag ggatggtcac gggacatgac gacattcctg tcactcagtc aggaggtgct
     2821 gttatccctg ctcagtttct gcaccgcatg cagccttaac ggtgtccaga cccgtgagtg
     2881 tggtcacacg tcacgcagtc cgcttgacac gctggaaagc gccatcggct ttcacatgcg
     2941 cgactggtgg cagccgacaa aagcaaactt cttcggacac ctgaaaaagc cgcagattat
     3001 cgcagccctg aatgaggcag ggctgtccgg tgccgcacgg gacgcggaga agatgaagaa
     3061 aggtgatgcg gctgaacatg cagagcacca tatgaaagac aaccgctggg tgcctggctg
     3121 gatgtgtgca ccacatccac agacagatac cactgaacgc accgataacc tggctgatgc
     3181 cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagcaagg gagataccgt
     3241 gatgaaaact gaactgaccc tgaatgtatt acagaccatg agcgcacagg aatatgaaga
     3301 tatccgggct gccggaagtg atgaacgtcg tgagctgaca cacgccgtga tgcgggaact
     3361 ggatgcgccg gacaactgga cgatgaacgg cgagtacggc agcgagtttg gcggattttt
     3421 ccccgtccag gtccgtttca cgcctgccca cgaacgtttt cacctggcgt tatgttcacc
     3481 gggcgatgtc tctcaggtct gggtgctggt tctggtgaat gccggtggtg agccgtttgc
     3541 ggtggttcag gtacagcggc ggtttgcacc ggaagccgtc agccattcac tggcactggc
     3601 ggcgtcactg gacgcgcagg ggtacagcgt cagtgacatc atccatatcc tgatggcgga
     3661 aggaggtcag gcatgagcgc acgttcacag gcactggttc ccctcagcgc agagcaacag
     3721 gccgcatggc gggcggtggc ggagacagaa aaacgccgcc accagggaaa cacgctggcg
     3781 gaatatccgt atgcgggggc gtttttccgc tgtctgaacg gaagccgcag gatatcgctg
     3841 tctgacctgc gatttttcat gccgtccctg accgcagaag aactgcacgg cagccgtctt
     3901 cagtggctgt atgccatcga tgtgctgatt gaaacacagg gcgaggtctg cctccttcct
     3961 cttccaggcg atgcagcaga acgcttgttc ccgtcggtgc ggtttcgtgt cagggaacgc
     4021 agtcgccata aatccgcgct ggtcatgcag aagtacagcc gccagcaggc gagggaggca
     4081 gagcagaaag cccgtgcgta ccaggcgctg gtcgctcagg ctgaaatcga actggcattc
     4141 cattcgccgg aaacagtcgg aagctggtat gcacgctgga gtgaccgggt ggcagagcat
     4201 gacctggaaa ccctgttctg gcagtggggt gagcgttttc cgtcactggc cggaatggaa
     4261 cgctggcagt ggcaggatat gccgttctgg caggttattg cggaagccag tcttgcagca
     4321 agagaggccg gtcacgctgt ccgtgagatg gagcggtgga tggtgccgaa caaactccgg
     4381 gaggcggcgt gatgcggcaa caatcacaca gattacccgt aaacagcctg aatgagcggg
     4441 ttattttcag gaaaagtgag tgtggtcagc gtgcagggat atgggctatg atgtgcccgg
     4501 cgcttgaggc tttctgcctc atgacgtgaa ggtggtttgt taccgtgttg tgtggcagaa
     4561 agaagatagc cccgtagtaa gttaattttc attaaccacc acgaggcatc cctatgtcta
     4621 gtccacatca ggatagcctc ttaccgcgct ttgcgcaagg agaagaaggc catgaaacta
     4681 ccacgaagtt cccttgtctg gtgtgtgttg atcgtgtgtc tcacactgtt gatattcact
     4741 tatctgacac gaaaatcgct gtgcgagatt cgttacagag acggacacag ggaggtggcg
     4801 gctttcatgg cttacgaatc cggtaagtag caacctggag gcgggcgcag gcccgccttt
     4861 tcagggctga tgctggtctg actgcactga agcgccttta taaaggggct gctggttcgc
     4921 cggtggcccc tttctccttg ctggcattgt acgggcatga acaaccagac tgccaggaag
     4981 gccgtttccc tccgggaacg gggatgaacg tgcgcctgcg gcgcccgttc ttttcccccg
     5041 ccttctctgt ttatgactta ccagaaatca catcaccttc cctttgatta ttctcttttt
     5101 cctcctgctc tgactctgat tactgctctg actctgatta ctgctgccct gattattgtc
     5161 tgttttgttg ttctcctgct tatgcctttt ccgggcgtcc ccctggcggg gcgggctgtc
     5221 gtgaacggag ccagcgaagc tgtctccgcc ctggcgggct tccatccctg acgcaaaaac
     5281 ccgggtttgc agcgaccgga acggtcgcgc cacccttgtc gcgccgtggc ctgcgctccc
     5341 ggcatcccgg caaaagacgc cgggatgtaa
//
LOCUS       DAZSNG010000186         5339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZSNG010000186 DAZSNG010000000
VERSION     DAZSNG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.190"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHW4Z_003951"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHW4Z_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR8883353.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHW4Z_003952"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHW4Z_003952"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR8883354.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHW4Z_003953"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHW4Z_003953"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR8883355.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHW4Z_003954"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHW4Z_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR8883356.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZSNG010000187         5328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZSNG010000187 DAZSNG010000000
VERSION     DAZSNG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.191"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..171
                     /locus_tag="ACHW4Z_003955"
     CDS             <1..171
                     /locus_tag="ACHW4Z_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883357.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHW4Z_003956"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHW4Z_003956"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR8883358.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHW4Z_003957"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHW4Z_003957"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR8883359.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHW4Z_003958"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHW4Z_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR8883360.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHW4Z_003959"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHW4Z_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR8883361.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1314 a         1187 c         1397 g         1430 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgcta
//
LOCUS       DAZSNG010000188         5182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZSNG010000188 DAZSNG010000000
VERSION     DAZSNG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.192"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..746
                     /locus_tag="ACHW4Z_003960"
     CDS             <1..746
                     /locus_tag="ACHW4Z_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8883362.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHW4Z_003961"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHW4Z_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR8883363.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3606..3809
                     /gene="safA"
                     /locus_tag="ACHW4Z_003962"
     CDS             3606..3809
                     /gene="safA"
                     /locus_tag="ACHW4Z_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR8883364.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3877..4638
                     /gene="ydeO"
                     /locus_tag="ACHW4Z_003963"
     CDS             3877..4638
                     /gene="ydeO"
                     /locus_tag="ACHW4Z_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR8883365.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5040..>5182
                     /locus_tag="ACHW4Z_003964"
     CDS             5040..>5182
                     /locus_tag="ACHW4Z_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR8883366.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1481 a         1103 c         1155 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaatgca tgcgaccaca gtgaaaaaca aaatcacgca aagagataac tataaagaaa
     3661 tcatgtctgt aattgtggtt gtcttattac tgacacttac gttgatagcc attttttcgg
     3721 caattgatca gctgagtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca
     3781 ttatcaatag tttgcaagac tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa
     3841 ggtatcgata aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata
     3901 tttattcatc atgctttcaa cgctaacatt ttagataaag attacgcctt ctccgacggc
     3961 gagatcctga tggtagataa cgctgttcgt acacattttg aaccttatga acggcatttt
     4021 aaagaaatcg gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag
     4081 acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg
     4141 cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa
     4201 cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta
     4261 actaacggtg tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg
     4321 cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag
     4381 aaaaaactaa agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag
     4441 cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt
     4501 tatgccagta catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag
     4561 agagtttcta aggagtaccg ttgtcaaagg cacacgggta tgaatacggg caacacgatg
     4621 agtgctttag ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa
     4681 ttatatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatatt tattgaaata
     4741 aataagtgac atccatcaca tatttatgca cttgcataac ctgttgcatg attatttatg
     4801 atctcaattc ggcattttgt cagtaaaatg caataattta ttaaatatca ataaattagt
     4861 tgtttatcgg cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg
     4921 ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc
     4981 cacttatttc tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta
     5041 tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg
     5101 gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa
     5161 acgttgcttt ctcagacttt ac
//
LOCUS       DAZSNG010000189         5163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZSNG010000189 DAZSNG010000000
VERSION     DAZSNG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.193"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..220
                     /locus_tag="ACHW4Z_003965"
     CDS             <1..220
                     /locus_tag="ACHW4Z_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883367.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHW4Z_003966"
     CDS             complement(392..721)
                     /locus_tag="ACHW4Z_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR8883368.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHW4Z_003967"
     CDS             complement(726..911)
                     /locus_tag="ACHW4Z_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR8883369.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHW4Z_003968"
     CDS             complement(908..3547)
                     /locus_tag="ACHW4Z_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR8883370.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHW4Z_003969"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHW4Z_003969"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8883371.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHW4Z_003970"
     CDS             4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHW4Z_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR8883372.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1307 a         1323 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aac
//
LOCUS       DAZSNG010000190         5151 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZSNG010000190 DAZSNG010000000
VERSION     DAZSNG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.194"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            190..447
                     /locus_tag="ACHW4Z_003971"
                     /pseudo
     CDS             190..447
                     /locus_tag="ACHW4Z_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACHW4Z_003972"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACHW4Z_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR8883373.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACHW4Z_003973"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACHW4Z_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR8883374.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACHW4Z_003974"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACHW4Z_003974"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR8883375.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="ACHW4Z_003975"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="ACHW4Z_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3215..3431
                     /locus_tag="ACHW4Z_003976"
                     /pseudo
     CDS             3215..3431
                     /locus_tag="ACHW4Z_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3605..4420)
                     /locus_tag="ACHW4Z_003977"
     CDS             complement(3605..4420)
                     /locus_tag="ACHW4Z_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8883376.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTCYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="ACHW4Z_003978"
     CDS             4517..>5151
                     /locus_tag="ACHW4Z_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8883377.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcacgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAZSNG010000191         5096 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZSNG010000191 DAZSNG010000000
VERSION     DAZSNG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.195"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(11..322)
                     /locus_tag="ACHW4Z_003979"
     CDS             complement(11..322)
                     /locus_tag="ACHW4Z_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR8883378.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHW4Z_003980"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHW4Z_003980"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHW4Z_003981"
     CDS             701..1054
                     /locus_tag="ACHW4Z_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR8883379.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHW4Z_003982"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHW4Z_003982"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR8883380.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHW4Z_003983"
     CDS             complement(2810..3280)
                     /locus_tag="ACHW4Z_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR8883381.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHW4Z_003984"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHW4Z_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHW4Z_003985"
     CDS             complement(4119..4337)
                     /locus_tag="ACHW4Z_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR8883382.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHW4Z_003986"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHW4Z_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR8883383.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1376 a         1176 c         1220 g         1324 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatg
//
LOCUS       DAZSNG010000192         5024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZSNG010000192 DAZSNG010000000
VERSION     DAZSNG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.196"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHW4Z_003987"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHW4Z_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR8883384.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHW4Z_003988"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHW4Z_003988"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR8883385.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="ACHW4Z_003989"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="ACHW4Z_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR8883386.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="ACHW4Z_003990"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="ACHW4Z_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR8883387.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACHW4Z_003991"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACHW4Z_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR8883388.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAZSNG010000193         4973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZSNG010000193 DAZSNG010000000
VERSION     DAZSNG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.197"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHW4Z_003992"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHW4Z_003992"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR8883389.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHW4Z_003993"
     CDS             1020..1703
                     /locus_tag="ACHW4Z_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR8883390.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHW4Z_003994"
     CDS             1700..2161
                     /locus_tag="ACHW4Z_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR8883391.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHW4Z_003995"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHW4Z_003995"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8883392.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHW4Z_003996"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHW4Z_003996"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR8883393.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHW4Z_003997"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHW4Z_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR8883394.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHW4Z_003998"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHW4Z_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR8883395.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHW4Z_003999"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHW4Z_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883396.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZSNG010000194         4870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZSNG010000194 DAZSNG010000000
VERSION     DAZSNG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.198"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHW4Z_004000"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHW4Z_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR8883397.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHW4Z_004001"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHW4Z_004001"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR8883398.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHW4Z_004002"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHW4Z_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHW4Z_004003"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHW4Z_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR8883399.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHW4Z_004004"
     CDS             4169..4342
                     /locus_tag="ACHW4Z_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883400.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHW4Z_004005"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHW4Z_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR8883401.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHW4Z_004006"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHW4Z_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR8883402.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHW4Z_004007"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHW4Z_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1246 a         1120 c         1209 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccgtac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZSNG010000195         4836 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZSNG010000195 DAZSNG010000000
VERSION     DAZSNG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.199"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..653)
                     /gene="mxiG"
                     /locus_tag="ACHW4Z_004008"
     CDS             complement(<1..653)
                     /gene="mxiG"
                     /locus_tag="ACHW4Z_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR8883403.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     "
     gene            complement(662..1120)
                     /gene="ipgF"
                     /locus_tag="ACHW4Z_004009"
     CDS             complement(662..1120)
                     /gene="ipgF"
                     /locus_tag="ACHW4Z_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR8883404.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(1120..1482)
                     /gene="ipgE"
                     /locus_tag="ACHW4Z_004010"
     CDS             complement(1120..1482)
                     /gene="ipgE"
                     /locus_tag="ACHW4Z_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR8883405.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(1492..3108)
                     /locus_tag="ACHW4Z_004011"
     CDS             complement(1492..3108)
                     /locus_tag="ACHW4Z_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR8883406.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            3422..>4836
                     /gene="icsB"
                     /locus_tag="ACHW4Z_004012"
     CDS             3422..>4836
                     /gene="icsB"
                     /locus_tag="ACHW4Z_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR8883407.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGT"
BASE COUNT         1550 a         1013 c          663 g         1610 t
ORIGIN      
        1 gggaatcgtt gattgatata ttgttgaatt ctatttattt ctttgtttga caccagaata
       61 tatctgcatt tggtaaatcc ggttttattt aaggctacag acgcccatac cgcatcattt
      121 tgtgtttcgg caagaatata attgcactgt ccagtgttga ctattccata tctcttatca
      181 tctataattt cagcaatttc ttttacttca tctttcttta aagagaggaa aaaaattata
      241 attaacacaa caataacagc aaagaaaaaa aacaccgagt ggttcttata cattccgttt
      301 agtatatggc ctctcgattt atcctccctc atatttttaa ggtgaaaaga aatcccgtca
      361 tattcaaatg aattaaattg tgaattgata tcaatttgct cacctttttc atcactaaat
      421 tgacagatat tatctttgct tatggtcaaa taaattatac catccgattt actatctgtt
      481 acaggaataa tattttctgg gaaattgtcg tttccaaact caagtgtttc tatcgtccgt
      541 ccgagaacaa ttaagttatt cccataatat aacggaaact catccccgac accgtttgtg
      601 agtttcacta ataacctgaa tggtgcaaga tttgagttct ttgcctcaga cataaatcct
      661 cttatatcct tcgattattc tgcttgctct ctgctgctat ccgcaaatag cgtctataga
      721 tatcctctgc gtactttaat ctttcttttt tctttttcgg agacgttcct gcattatagg
      781 ctccaacagc ctcccaccct cttccataca tcatcataaa ctcatttaat aactttgctg
      841 cataatgtac agaaagacat ggatgactaa ttagcatttc ctcagaatat cccatttctc
      901 taagtctttt agaatgaaag tcatttattt gcattatacc ataatcttta cttccattgt
      961 tattaacatt gactgcagat ttattaaatc cggattcttt ttccgcaatc gcttttaaca
     1021 aacttgatgg aatattgtac ctttcaccag ccttatccca acaatcagct ctccctaaat
     1081 atgggataaa acacagcaaa ataaatacga aacgggacat taatacccct tcattcttcg
     1141 cgcaaattca tccttcagcc aacgcacacg cgaaatataa tattctgtat taacatatac
     1201 atcctcgaat tcatttatcc cggtaagatc taatcgcgca attagatttc cgccttcatc
     1261 atcagtagct aagcatatct tttctgagta atttaggctc aacgcatata taagatcatt
     1321 tatattttca ggcaatgcac aaaaagggca taataaattt attgaatctc cttctttttc
     1381 aatatatatt agcacatcat catcaagcat gattgcttga tcatcgatat caattaaagg
     1441 gatacccaag gctcggcaaa taacatctgc taaatcttcc atatattcct cttatacaaa
     1501 tgacgaatac cctttcacca tattccatat ttttgggtcc cctattcttt cagaataaga
     1561 tagctctaac gaagatagcg gcaatttttt catgacttta tttccgggca caccagtatt
     1621 catctcttgg atttccatat taccactatt cattagaata gtagaaaata atcttttctc
     1681 ttctgaggat aatttactat ttaattgaga aaactgacct gtttcatgtt ttcttattat
     1741 ttctctttta atttcagcat cctgcatacc tgttctgtcc ttcccactct tacaattcca
     1801 gcaaggtaca gcacctatag tataagccaa tagagtcatt ctttgtgaca atttatacgg
     1861 ctctccatta tcattttttt gtaacttttt ggttacaatc tcttttatct ggttagccag
     1921 atatatcaca tcattcttac atggtggatt tttttctatt gcttctgcag cccaacctcc
     1981 aatcacacca tttttgagga aattatcccc caacaaagaa catattgatt catcattaag
     2041 cttgtcaaca ttcctccagc ctaagcccat ttttagcgct aattcattta caccaaagtt
     2101 aaatgtcacc acttttagat taacgcttac ttctttcaga agaccatcac tattccgaat
     2161 taataatttc gttggcttcc cccttttaga gtttaaccct tttaatgcgt tcacctgatc
     2221 ttttaacata ctctcctctc ccccggttaa actggtcggc gtcagaagag aagtcgaaac
     2281 aatctttaaa tctactgttt taccagacaa agcctgtgat aataactctg gtctgctata
     2341 taatgctgcg cttactaact cctcagcttt attacgagca gcaacagctc tttcagatga
     2401 gttttttttc aatccatagg ctgatataac tccatgtctg atacccgaaa aaatttcctt
     2461 cccctcatca tccaccactt ttgaaagcca catattcgcg atatgatcac tctctctcgt
     2521 agaagcacaa caaattcctt ttccattata ctctttgaca aatatatttt tattccctat
     2581 cttcatatga gaggcaggag ttaattgaaa accataattt ttcccatgat gactaatatt
     2641 cttgtcgacc ggtccccaat tcttattatt taaataatca gtaaatattt gttttatttt
     2701 cgtttgcaat tcctttttat ttttaatgcc tagctgtgct cttatctctt cacttattac
     2761 tttattaagc tcattaatat gtcttttatt aacttcacca agtttatagg tatctaatcg
     2821 ttccatcatg gctttaattt ttgacgtatc accacctgcc aactttattg cctcgcaaaa
     2881 aacttcagct ctgtcacgcg caaacaaaag gctttttcct gttaagcttg tttggttctg
     2941 taaatataat cgattaagcg cttcgatccc cttatttctt tctccatttt taactctttg
     3001 atatgaaatc acgcttacac ctttgtgctt tccggtttct tctgcgcctt tataggaatc
     3061 tccgctttga aatgaaacct gatgcaatcc caaattagtt atgtgcattt aattatcctc
     3121 agtcataaat ggggcattga ttgcagtttt gtacctcgtt ttacaacaaa aaagatactt
     3181 attcaacagg gttctctctt tttctataac actccgagcc tgatacatca ggcactttat
     3241 cttgtgggat ttcatgatga aacgagcact acatatgctc acgaggtaca tattatgtac
     3301 agcaaaagaa aatttgatta tgttaacagg ctcaagtttc taaatttatt aattgaatat
     3361 attatatacc aaatacattt tacatcccca caatcaccaa gtaatggaga gttaataaag
     3421 tatgatcctc aaaattagca atttcattga cgcaagcaat acaaaagggc ctatacgcgt
     3481 tgaagataca gagcatggac ctatattgat agcacagaaa ttcaacctaa aggatctctt
     3541 tttcagaaca ttaagcacta ttaatgcaaa gataaattca cagatattaa atgaacaact
     3601 aaaaaattat cgactagaga atcaaaaatc cttattgctt ttcctcaata cattagctag
     3661 cgaaaaatct gcagaaagtg cgtttgccgc ctatgaagca gctaaaaact caattcaaca
     3721 ctctttcaca ggtagagaca tcaaattaat gttgaatacc gcagagcgtt ttcatggcat
     3781 cggtacagcc aaaaatcttg aaagacattt agtttttcgt tgctggggaa atagaggcat
     3841 aacccattta gggcatacta gtatcagtat aaaaaacaat ttactacagg aaccaactca
     3901 tacctatctc agctggtatc ccggcgggaa tgttacaaaa gatacagaaa taaactacct
     3961 ttttgaaaaa cgctcaggtt acagtgtcga tacctataag caagacaaac taaatatgat
     4021 ttcagaccaa acagccgaaa gacttgatgc aggccaagag gttagaaact tattaaattc
     4081 aaaacaagat caaaataaca acaaaaaaat attttttccc agagctaatc aaaaaaaaga
     4141 cccctatggt tattgggggg tcagtgctga taaagtatac attccattat caggtgataa
     4201 taaaacaaag gatggtaaaa tcagccataa cctttttggc cttgatgaaa caaacatgtc
     4261 aaaatttata tgcaaaaaga aagcagatgc tttcagacaa ttggcaaatt ataaattaat
     4321 cagtaaatct gaaaactgtg ctggtatggc acttaatgtt cttaaagcag gaaattctga
     4381 aatatacttt ccactccctg acgttaaatt ggttgctaca cctaacgatg tatacgcgta
     4441 tgcaaataaa gttaggcaac gcattgaaag tctcaatcag tcttataacg aaataatgaa
     4501 gtatattgaa tctgattttg atctttccag attgactcaa ttacgacgca gctatcttaa
     4561 aagtttcaat aaaattaacc ttatccacac accaaaaaca ttcaagcctt tatcaatttc
     4621 actttacaaa catcctactg aaaatgtatc ttcagaagac tttgatgcgg tcatcaacgc
     4681 ttgccactca tacttagtaa aatcagcacc cagcaatatg actcgtgtat taaacgaatt
     4741 aaaaaccgaa gcaacagata aaaaagaaga aatcatcgaa aaatcaataa aaattattga
     4801 ttattacaat tctcttaaat cgccagatct tggaac
//
LOCUS       DAZSNG010000196         4783 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZSNG010000196 DAZSNG010000000
VERSION     DAZSNG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.200"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="ACHW4Z_004013"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="ACHW4Z_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR8883408.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="ACHW4Z_004014"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="ACHW4Z_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR8883409.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="ACHW4Z_004015"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="ACHW4Z_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR8883410.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="ACHW4Z_004016"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="ACHW4Z_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR8883411.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4783)
                     /locus_tag="ACHW4Z_004017"
     CDS             complement(4148..>4783)
                     /locus_tag="ACHW4Z_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8883412.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1268 a         1169 c         1129 g         1217 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt atcgtcaggt
     4621 ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc agagctgcca
     4681 gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg ctctgtcctt
     4741 tttcactttc cattaatgca ttgaattctg ttttagataa cgg
//
LOCUS       DAZSNG010000197         4739 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZSNG010000197 DAZSNG010000000
VERSION     DAZSNG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.201"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1628
                     /gene="mxiD"
                     /locus_tag="ACHW4Z_004018"
     CDS             <1..1628
                     /gene="mxiD"
                     /locus_tag="ACHW4Z_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR8883413.1"
                     /translation="DSHLLEQNDIAKYVAQSDTVGSFFERFSALLNYPIVVSKQAAKK
                     RISGEFDLSNPEEMLEKLTLLVGLIWYKDGNALYIYDSGELISKVILLENISLNYLIQ
                     YLKDANLYDHRYPIRGNISDKTFYISGPPALVELVANTATLLDKQVSSIGTDKVNFGV
                     IKLKNTFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAQNDPMPPFNITQKV
                     SEDSNDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDIAKRHI
                     ELSLWIIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMAL
                     NQKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFS
                     SRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYT
                     HETNSNEIISIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIKESSYYNTAEYKS
                     LISEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
     gene            1647..2714
                     /gene="mxiC"
                     /locus_tag="ACHW4Z_004019"
     CDS             1647..2714
                     /gene="mxiC"
                     /locus_tag="ACHW4Z_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR8883414.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            2727..>4739
                     /gene="mxiA"
                     /locus_tag="ACHW4Z_004020"
                     /gene_synonym="virH"
     CDS             2727..>4739
                     /gene="mxiA"
                     /locus_tag="ACHW4Z_004020"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR8883415.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISY"
BASE COUNT         1553 a          657 c          948 g         1581 t
ORIGIN      
        1 ttgactctca tttattagaa caaaatgaca tagcgaaata tgttgctcaa agtgatacgg
       61 taggatcttt ttttgaacgt ttttctgcgt tattaaatta tcctattgtt gttagcaaac
      121 aagctgcaaa aaagagaata tctggggagt ttgatttatc taatccagaa gaaatgcttg
      181 aaaaactgac attacttgtg ggactcatat ggtataaaga tggaaatgca ttatacatat
      241 atgactctgg agaattaata agcaaagtta ttttgctgga gaatatatca cttaattatc
      301 ttattcagta tctgaaggat gctaaccttt atgatcacag atatcctata agaggaaaca
      361 ttagtgataa aacattctat atatctggtc ctccggcatt ggttgaactg gtagctaata
      421 cagcaacttt gcttgataag caggtttctt ctattgggac agataaagtt aattttgggg
      481 ttataaaatt aaaaaataca tttgttagtg acagaactta taatatgaga ggggaggata
      541 ttgtcatccc aggggtggct actgttgtag aacgtttatt aaataatggt aaggctctga
      601 gtaacagaca agcccaaaat gatccgatgc caccctttaa tattacacag aaagtatctg
      661 aggatagtaa tgacttttca tttagttcag ttaccaatag ttctatatta gaggatgtat
      721 cacttattgc atatccagaa accaattcta tattagtcaa aggtaatgat cagcaaatac
      781 agattattcg cgatattatc actcagttag atatagccaa acgacatata gaattatcat
      841 tatggataat cgatattgat aaatctgaat taaataacct tggtgttaat tggcaaggaa
      901 cggcttcatt tggtgattcg tttggtgcgt catttaatat gagctcatca gcatcgataa
      961 gcactttgga cggcaataag tttattgctt cagttatggc attaaatcag aaaaagaaag
     1021 caaatgtagt ttcaaggccg gtgattttga cacaagagaa tattccggct atatttgata
     1081 ataacaggac tttttatgtg tcattagttg gtgagcgcaa ttcttcgttg gagcatgtta
     1141 cgtatggaac attgataaat gttatacctc gcttttcatc tcggggacaa attgagatgt
     1201 ctttaacgat agaggatgga acaggaaata gtcagtcaaa ttacaattat aataatgaaa
     1261 atacatctgt ccttccagaa gttggaagga ccaaaatcag tacaatagcg agagttccac
     1321 aaggcaaaag tttgttgata ggtggttata cgcatgaaac gaactctaac gaaataatat
     1381 ctattccttt tctctcctct attcctgtaa tcggtaatgt gttcaagtat aaaacaagta
     1441 atatcagtaa tattgtaagg gtttttctga ttcaaccccg tgagataaag gagtcaagtt
     1501 attataatac tgcagaatat aaaagcctca tatctgaaag agaaatacaa aagactacac
     1561 aaataatccc atctgaaact actttgctgg aagacgaaaa atcattggtt tcatacttaa
     1621 attactaact ataaagtagg tgatgtatgc ttgatgttaa aaatacagga gtttttagct
     1681 ctgcattcat tgataagttg aatgcaatga caaattcaga tgatggagat gagactgctg
     1741 atgcagagct tgattctggc ttggctaata gcaagtatat tgactcatct gatgagatgg
     1801 cttccgctct ttcgtcattt ataaacagaa gagaccttga gaaactgaaa ggaacaaata
     1861 gtgatagtca ggaacgtatt ttagatgggg aagaagatga aattaatcac aagatttttg
     1921 atttaaagag aacgttaaaa gataaccttc ctctagatcg ggattttata gacagactaa
     1981 agagatattt taaagatcca agtgatcaag tcttagcatt aagggaactt ttgaatgaaa
     2041 aagatcttac tgctgaacaa gtcgaattat taactaaaat tattaatgag ataatatcag
     2101 gtagtgaaaa aagtgttaat gctggaataa attcagctat acaggctaaa ttatttggca
     2161 acaaaatgaa acttgaacca cagcttttgc gtgcatgtta tcgtggtttt atcatgggga
     2221 acatatcaac aacagatcag tatatagaat ggcttggtaa ttttggtttt aatcacagac
     2281 atacaattgt gaattttgta gagcagtcac tgattgtaga catggattct gagaaaccga
     2341 gctgtaatgc ttatgagttt ggttttgtgt tatctaaatt aattgcaatt aagatgatta
     2401 gaacttcaga cgtaattttt atgaagaaac tggaatcctc aagcttgcta aaagatggca
     2461 gtttaagtgc agagcagcta ttgctaactt tattatatat ttttcaatat ccaagtgaaa
     2521 gtgagcaaat tcttacttct gttatagaag tatcacgagc cagtcatgag gattctgtag
     2581 tgtatcaaac atatctatct tctgttaatg aaagtcctca tgatatattt aaaagtgaaa
     2641 gtgaaagaga aattgcgatc aatattctac gagagcttgt cacaagtgca tacaagaaag
     2701 agctttctag ataacaggag ataaaagtga tccagtcttt tcttaagcaa gtaagtacta
     2761 agcctgaatt aattatatta gtgcttatgg ttatgatcat tgctatgttg atcataccat
     2821 tacctacata ccttgttgat tttttaatag ggcttaatat tgtacttgct attctcgttt
     2881 ttatgggatc attttatatt gaaagaatat tgagtttttc tacattccct tcggttttgt
     2941 tgataacgac attatttcgt ctcgctttat caataagtac tagtcgacta attttagttg
     3001 atgcggatgc gggtaaaatt attactacat ttggccagtt tgtcattggt gatagtctgg
     3061 ctgtaggttt tgttattttt tcaatagtaa ctgttgtgca gttcattgtt ataacaaaag
     3121 gatctgaaag ggttgcggag gtagctgctc gcttttctct tgacggtatg ccggggaaac
     3181 aaatgagcat tgatgcggat ttaaaggctg gaattattga tgccgcagga gctaaagaaa
     3241 gacgcagtat tctggaacgt gagagtcaat tgtatggttc atttgatgga gcaatgaagt
     3301 tcattaaagg tgatgcaatt gctggcatca ttattatttt tgtcaattta ataggaggta
     3361 tttctgttgg tatgagtcaa catggaatgt ccctatctgg tgcattatct acttatacca
     3421 tactaactat tggtgacgga ttggtctctc agattcctgc tctgttaatt tctataagtg
     3481 caggatttat cgttacaaga gttaatggtg atagcgataa tatgggacgt aacataatgt
     3541 cccagatatt tgggaatcct tttgttctca ttgttacatc agctcttgcc ttggcgatag
     3601 ggatgttgcc aggttttccg ttttttgttt ttttcctgat agcagttact ttgacggctt
     3661 tattctatta taaaaaggtc gtagaaaaag aaaaaagtct gtctgagtct gattctagtg
     3721 gctatactgg tacatttgat attgataata gccatgactc atctttggca atgatagaaa
     3781 atctggatgc tattagttca gaaaccgttc ctttaatttt attatttgcc gaaaataaga
     3841 taaatgcaaa tgatatggaa ggtcttattg aaaggataag aagtcagttc tttattgatt
     3901 atggtgtcag gcttccaact attttatata gaacaagcaa tgagcttaag gtcgatgata
     3961 ttgttttgtt aataaatgag gtgcgtgccg atagttttaa tatatatttt gacaaagtgt
     4021 gtattacaga tgaaaatgga gatatagatg ctctaggtat tcctgttgtt tcaacttcat
     4081 ataatgaacg tgttatttct tgggtagacg tttcatatac agaaaatcta actaatattg
     4141 atgctaaaat taaaagtgct caagatgaat tttatcacca gttgtcacaa gctttattaa
     4201 acaacataaa tgagattttt ggtatacaag aaacaaaaaa tatgttagat cagtttgaaa
     4261 atcggtatcc tgatctatta aaggaagtct tccgacatgt gactatacag agaatttctg
     4321 aggtattaca aagattgctt ggagaaaata tttctgttcg caatttaaaa cttattatgg
     4381 agtctttggc gctttgggct cccagagaaa aagatgtcat aacattagtt gaacatgtcc
     4441 gtgcatcact ttctaggtat atttgtagta aaatagctgt ttctggtgag attaaagttg
     4501 tgatgctttc cggatatatt gaggatgcaa taagaaaagg gataaggcaa acctctggtg
     4561 gctctttctt gaatatggat atagaggttt cggatgaggt aatggaaact ttagcacatg
     4621 ctttgagaga attgagaaat gcaaaaaaaa atttcgttct tttggtatca gtagatatac
     4681 gtaggtttgt taaaagactt atagataaca gatttaagag tatactcgtt atatcgtat
//
LOCUS       DAZSNG010000198         4603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZSNG010000198 DAZSNG010000000
VERSION     DAZSNG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.202"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHW4Z_004021"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHW4Z_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR8883416.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHW4Z_004022"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHW4Z_004022"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR8883417.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHW4Z_004023"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHW4Z_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR8883418.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHW4Z_004024"
     CDS             complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHW4Z_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR8883419.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAZSNG010000199         4552 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZSNG010000199 DAZSNG010000000
VERSION     DAZSNG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.203"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACHW4Z_004025"
     CDS             <1..1224
                     /locus_tag="ACHW4Z_004025"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR8883420.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHW4Z_004026"
     CDS             1224..1958
                     /locus_tag="ACHW4Z_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8883421.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..3186
                     /locus_tag="ACHW4Z_004027"
     CDS             2425..3186
                     /locus_tag="ACHW4Z_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8883422.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3260..4552
                     /locus_tag="ACHW4Z_004028"
                     /pseudo
     CDS             3260..4552
                     /locus_tag="ACHW4Z_004028"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1247 a          866 c         1121 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa
     3181 tcataatgta ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg
     3241 actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc
     3301 atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca
     3361 gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga
     3421 tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg
     3481 tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac
     3541 tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta
     3601 tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa
     3661 aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta
     3721 tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg
     3781 ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac
     3841 atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg
     3901 aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt
     3961 gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc
     4021 ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat
     4081 tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct
     4141 ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa
     4201 tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg
     4261 atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct
     4321 gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga
     4381 gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg
     4441 ctgttaggga gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat
     4501 aaatatcgta ttcgttatag taaatccatt agtgattgga tttgccccta ta
//
LOCUS       DAZSNG010000200         4317 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZSNG010000200 DAZSNG010000000
VERSION     DAZSNG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.204"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..117
                     /locus_tag="ACHW4Z_004029"
     CDS             <1..117
                     /locus_tag="ACHW4Z_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883423.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(114..380)
                     /locus_tag="ACHW4Z_004030"
     CDS             complement(114..380)
                     /locus_tag="ACHW4Z_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR8883424.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="ACHW4Z_004031"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="ACHW4Z_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR8883425.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            complement(4134..>4317)
                     /locus_tag="ACHW4Z_004032"
     CDS             complement(4134..>4317)
                     /locus_tag="ACHW4Z_004032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883426.1"
                     /translation="LYAFIVTDFIFCWAIALPLIFQHIKRQARTVVRAFFLELDNPYP
                     EHLIGVAFFRFSGHLC"
BASE COUNT         1049 a         1134 c         1123 g         1011 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaacttct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataac
//
LOCUS       DAZSNG010000201         4255 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZSNG010000201 DAZSNG010000000
VERSION     DAZSNG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.205"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2094
                     /locus_tag="ACHW4Z_004033"
     CDS             <1..2094
                     /locus_tag="ACHW4Z_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR8883427.1"
                     /translation="PDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQL
                     QAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPS
                     QVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGR
                     TPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRL
                     STWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPD
                     KIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQH
                     IDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQ
                     GWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGG
                     DSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSG
                     DAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVW
                     GDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGK
                     TEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREVARENLLQERLQQMER
                     DMVRDLQKEKTLGGD"
     gene            2114..2860
                     /gene="traX"
                     /locus_tag="ACHW4Z_004034"
     CDS             2114..2860
                     /gene="traX"
                     /locus_tag="ACHW4Z_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR8883428.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            2915..3475
                     /locus_tag="ACHW4Z_004035"
     CDS             2915..3475
                     /locus_tag="ACHW4Z_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR8883429.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3607..3819
                     /locus_tag="ACHW4Z_004036"
     CDS             3607..3819
                     /locus_tag="ACHW4Z_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR8883430.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            4065..>4255
                     /locus_tag="ACHW4Z_004037"
     CDS             4065..>4255
                     /locus_tag="ACHW4Z_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8883431.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWST"
BASE COUNT          989 a         1037 c         1397 g          832 t
ORIGIN      
        1 ccggatttca gcaacacgct gtttctgctc gatgagagct caatggtggg caataccgac
       61 atggcacggg catacgccct gattgcggcc ggtggcggtc gtgctgtggc cagcggtgac
      121 acggaccagc tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt
      181 gctgccgatg tggccatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg
      241 gtatacagcc tgattaaccg ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa
      301 ccgtctcagg tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc
      361 agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aggcgatgct gaaaggcgag
      421 gctttcccgg atatccccat gacactgtat gaggccattg tccgcgacta taccggcagg
      481 acaccggaag cacgggagca gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta
      541 ctgaacagca tgattcatga tgcacgggaa aaggccggtg agctgggcaa agagcaggtc
      601 atggtgcctg tcctgaacac agcgaatata cgtgacggag aactgcgtcg tctctccacc
      661 tgggagaaaa acccggatgc ccttgccctg gtggatagtg tgtatcaccg gattgccggt
      721 atcagcaagg atgacgggct gataaccctg caggatgcgg aaggtaacac gcggctgatt
      781 tcgccccggg aggcggtggc tgaaggtgtc acactgtaca ccccggacaa aatccgggtg
      841 gggaccggcg accggatgcg cttcacgaag agtgaccggg agcgcggtta tgtggccaac
      901 agcgtctgga cggtgacagc ggtttccggt gacagtgtca cgctgtcgga cggacagcag
      961 acccgggtga ttcgccccgg tcaggagcgg gcagagcaac atattgacct ggcctatgcc
     1021 atcactgccc acggtgcgca gggggcgagt gaaacctttg ccatcgcgct tgaaggcacg
     1081 gaaggtaacc ggaaactgat ggccggcttt gagtcagcct acgtggccct gtcgcgtatg
     1141 aagcagcatg tgcaggtgta caccgataac cgtcagggct ggacggatgc cattaacaat
     1201 gccgtacaga aaggaactgc ccacgatgtg ctggagccga aaccggaccg ggaggtcatg
     1261 aatgcgcagc ggctgttcag tacggcacgg gaactgcggg acgtggcggc aggccgtgcc
     1321 gttctccgtc aggcggggct ggccggggga gacagtccgg cacggtttat tgcaccgggg
     1381 cgcaaatatc cgcagccgta tgtggcactg cctgcttttg accgtaacgg caagtccgcc
     1441 ggtatctggc tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt
     1501 gaaggacggg tgaaaggcag cggggatgcg cagtttgtgg ccctgcaggg cagccgtaac
     1561 ggcgagagcc tgctggctga taatatgcag gatggtgtcc ggattgcccg tgataacccc
     1621 gacagcggtg tggtggtgag aatcgccggt gaaggtcgtc cgtggaatcc cggtgccatc
     1681 accggtggtc gcgtgtgggg ggatatcccg gacaacagcg ttcagccggg agccggaaat
     1741 ggcgaaccgg tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt
     1801 gaaacggaac gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg
     1861 ccggacggca aaacagagca ggctgtcagg gagattgccg gacaggagcg tgaccggact
     1921 gccacttctg aacgggaagc cgcgctgccg gagagtgtgc tgcgtgaatc acaacgggag
     1981 cgggaagcgg tccgggaggt tgcccgggaa aatctgctgc aggagcgtct gcagcagatg
     2041 gagcgggata tggttcgtga cctgcagaaa gagaaaaccc tgggcggaga ctgatacagg
     2101 aagataaacg ctgatgacaa cggataacac gaacacgaca cgtaacgatt cactggctgc
     2161 acggaccgat acctggttgc agtcatttct ggtctggtca cccggacagc gggatatcat
     2221 caaaacggtg gcactggtgc tgatggtact ggaccacatc aatctgatat tccagctgaa
     2281 gcaggaatgg atgtttctgg ccgggcgagg agcctttccg ctgtttgccc tggtgtgggg
     2341 gctgaatctg tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg
     2401 ggggattatt gcccagttcg catattatct ggcaggcttt ccctggtatg aggggaatat
     2461 cctgtttgcc tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg
     2521 gcgtacagca gcggccattc ttctgatggc cctgtggggg cctttgtccg gcaccagtta
     2581 cggcattgcc gggctgctga tgctggcagt cagccaccgg ctgtaccggg cggaagacag
     2641 agcggaacgt ctggcgctgg tcgcctgtct gctggctgtt attccggcgc ttaaccttgc
     2701 caccagtgat gcagcggcgg tggctggtct tgtgatgacg gtgctgaccg ttggtctggt
     2761 gtcgtgtgca ggaaaatcat taccccgttt ctggcccggg gattttttcc cggtattcta
     2821 cgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga cgggtgtggt atctttgacc
     2881 gtaacaggat gattcgtcag aggcagaaca cagcatgaca gagcagaagc gaccggtact
     2941 gacactgaag cgaaaaacgg aaggggagac gcctgtccgc aggcggaaaa ccatcatcaa
     3001 tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag ctggccgaga aagcagcccg
     3061 ggaagcggag ctggcggcaa aaaaagcgca ggccagacag gcgctgtcca tttatctgaa
     3121 cctgccctcg ctggatgagg ccgtgaacac cctgaaaccc tggtggccgg gattatttga
     3181 cggtgaaacg ccccggcttc tggcctgcgg tatccgggac gtgttactgg aagacgtggc
     3241 gcagcggaat atcccgctct cgcataaaaa actgcgcagg gcgctgaagg ccatcacccg
     3301 ttcagaaagc tatctgtgtg ccatgaaagc cggtgcctgc cggtatgata cggaagggta
     3361 tgtgacggag catatttctc aggaggagga agcgtatgcg gcagcgcgtc tggataaaat
     3421 ccgccgccag aacaggataa aggcagagct tcaggccgtg cttgatgaga aataaaaaaa
     3481 agcctccatc agagaggagg cagggaaata aggcttaaaa ggaaataact tccgtacccg
     3541 aaaggaagcc aattcagtag attatttaac accataatac tgacgggtta aagaggagtg
     3601 ataaagatga atggattcag aaacagttca cggaacggtc aggtctggcg ctggcagcgt
     3661 gccggaagcc gggccgttat tctggaggtc agtggacgct ggatggaagc ggcagaagca
     3721 tggcgacggg ctgccggtgt tgcgccccgg acagactggc agcagtttgc ccgaaaaagg
     3781 gctgaacact gccaccggcg cagccgggga agggtgtaac gaaaaaagca gccttcatta
     3841 ctccagggat tcagaaggct gaataatgca tgagatttgc tttttttaat atccggtcgg
     3901 gcagtatgac cggacagttc tgagtagcac agagtttttt ccgtattaag tcgtgatttt
     3961 atattttgtg attaatttca caaaataagg tgttattcag tgtgtgctgc gatattcagg
     4021 attacctgaa atacaggtgc catttatctg atatggagaa taacatgagg aaatatattc
     4081 cactggtatt atttatcttt tcatggccgg tattgtgtgc agatattcat ggacgggttg
     4141 ttcgtgtact ggacggtgat accatcgagg tcatggactc acggaaggca gtgcggatca
     4201 ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg tcaac
//
LOCUS       DAZSNG010000202         4229 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZSNG010000202 DAZSNG010000000
VERSION     DAZSNG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.206"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACHW4Z_004038"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACHW4Z_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR8883432.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="ACHW4Z_004039"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="ACHW4Z_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            821..2014
                     /locus_tag="ACHW4Z_004040"
     CDS             821..2014
                     /locus_tag="ACHW4Z_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR8883433.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(2187..2672)
                     /locus_tag="ACHW4Z_004041"
     CDS             complement(2187..2672)
                     /locus_tag="ACHW4Z_004041"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8883434.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            complement(2660..2944)
                     /locus_tag="ACHW4Z_004042"
     CDS             complement(2660..2944)
                     /locus_tag="ACHW4Z_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8883435.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            3846..4010
                     /locus_tag="ACHW4Z_004043"
     CDS             3846..4010
                     /locus_tag="ACHW4Z_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883436.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
BASE COUNT         1072 a         1090 c          962 g         1105 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggatttg gtcttcgggc
     1741 cagcaaaagt cgttcaacag gaagaattct ggttctgagt ctgctggcaa cgttgagcac
     1801 tatcgtaatg tggttactcg gttatcacgc tgaaaataaa gggttacacc tgaaatatca
     1861 ggctaacagc attaaatccc ggcgggttat ctcatatctg acgttagcga agaatgttct
     1921 tcgacactcg ccacttattt taagacgaac agtactgagc acagttctaa accatctgtc
     1981 cagaacctac cgaaatatgg tgctggttta ttagcagtaa ttttgtgggg atccctcagc
     2041 ccttataggg ggcattctga aaagccacct cctcaggagg tggtctttta ctgtgcacag
     2101 aaaaatccgg aaacgagtga gcgtttccgg attcttacac agccactgga tcggtcaact
     2161 gatccttaac tgatcggcat tactccctat tgagggagtt taagaaacaa tgtttgcacc
     2221 tgagtttttg atggcttaaa accatgatga atataaaact gttttgcgtt ttccgtaagc
     2281 gcatgaacca taattgcacg cacaccgata ttttcggcaa ccctgtaaca acgacgaact
     2341 gcatcatgaa gtaaatccgc gccaagccct tttcctcgaa atgacacatc tacagccagg
     2401 cgtgcaagta taattacagg aatggggtct ggcatattgc gccgaaggtt acctgtagct
     2461 tctgtatgat tgacactacc agtggccagt gagtaaaaac cgactatttg ttgcgtacct
     2521 tttctgcata caacaaaagt ccgggtggct ccgattgcct gattttttag ctccttctgc
     2581 tttaaccagt catcaagtac agcttcactg ctgacaaatt cagcgacctg atgaaatgag
     2641 gataaaggtt ctggtgcagt tacacatccc attgaggttt ccttgccagc aatttttcga
     2701 tggcaggatc atctgcaacc ggagcatcaa gcatttcaat gaactcttca tattgcctgt
     2761 cattaaaatt aaacacccgc cggtcgagaa taacatcttc agcagcttta caggccattt
     2821 ccagaatgaa atctgtacgc gacttatgaa gaatttctgc agcagcatca atgagtatcc
     2881 gctgtgattc tcttgctctg agattgagct gaacacctga tttcataaag cctccgtata
     2941 tcatttgctt tacaacaata gcatattgac aatatatagc aaatgatata cacaaagtaa
     3001 cagattacag atgccgcgtc agcggcattt acctctgtgg acgtaaccgc actcaaaggg
     3061 gatatcgggt ggcgcagggg caccgccccc ctacgctccc acggagccgt acgtgacact
     3121 ctcgcgtcat acggctcttg tcgttgaacc attcaagtat cgtaagctaa gcaccagtgt
     3181 gcaaacagcg tcgggcagtc gtgacgaatt cgcagtaacc actcgaatgc acgcaccaga
     3241 ctacctcgcg ctcgcttgtg tttgcgggca atccacttgg ccaagtgcag gtcaacctgc
     3301 gccattaccg ggtatagctt cgattggtaa cctcaaacgc ctcgactgac tgcccatcga
     3361 cacctgccgc acctcgatta gctttgaccc gctggtatgc tttccaaacc gcatacttag
     3421 agacctcgaa cggctttggc tgactcatgg gctcctcccg gctatccggt tgaccgataa
     3481 accagcccca acgactgacc cccttcgctc catgcccatt acaggcactt cctcactact
     3541 acgaagccat ctgcccctgt gtctcgcatc ggtactcagg cgcttgcagt gcctctgctc
     3601 cgcgccgctc ccttagcatc gagacgacag gttcccacgt tccgtacgag agcctgtgtt
     3661 gcgttcatgc tgcctatatg ccggatgccg cccagccagt accagatatc tgctggactc
     3721 gtcatgcgct gcgtagatag cacattttcg acatcgccta gaggttcctt tcgacacttc
     3781 atcgacagtt cactttcgtt catctccgca acacctacct gacacatgtt ctgcgccttt
     3841 taaccttgac gctcaccacc accgctctta acggcagcag ctcaaggcgg tttgaaggct
     3901 gcgtctggac gccgcccctc atctctcata cagcattgca tccaacatca cgccactgga
     3961 tgcattcgtg gcacacctac gccggaaacg ccgaaattcc cggcctgtga tttttagtgg
     4021 tccttcggta cttcagtaac tccatcagct atcccctgag caagtcgttc catccatttc
     4081 gcaaatcgaa aatacccggg gtattgcaga caaatataat ccatctgaac ttttgaggtg
     4141 ctgtccatca cccgtttaaa gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4201 ttccacaaaa ataatgtcca tcattttga
//
LOCUS       DAZSNG010000203         4209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZSNG010000203 DAZSNG010000000
VERSION     DAZSNG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.207"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACHW4Z_004044"
     CDS             complement(<1..209)
                     /locus_tag="ACHW4Z_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8883437.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHW4Z_004045"
     tRNA            complement(324..399)
                     /locus_tag="ACHW4Z_004045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHW4Z_004046"
     tRNA            complement(406..480)
                     /locus_tag="ACHW4Z_004046"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHW4Z_004047"
     tRNA            complement(597..681)
                     /locus_tag="ACHW4Z_004047"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHW4Z_004048"
     tRNA            complement(690..765)
                     /locus_tag="ACHW4Z_004048"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHW4Z_004049"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHW4Z_004049"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR8883438.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHW4Z_004050"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHW4Z_004050"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR8883439.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHW4Z_004051"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHW4Z_004051"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR8883440.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZSNG010000204         4198 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZSNG010000204 DAZSNG010000000
VERSION     DAZSNG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.208"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHW4Z_004052"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHW4Z_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR8883441.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHW4Z_004053"
     CDS             complement(995..1690)
                     /locus_tag="ACHW4Z_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR8883442.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHW4Z_004054"
     CDS             complement(1683..3110)
                     /locus_tag="ACHW4Z_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR8883443.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHW4Z_004055"
     CDS             complement(3121..3840)
                     /locus_tag="ACHW4Z_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR8883444.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZSNG010000205         4185 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZSNG010000205 DAZSNG010000000
VERSION     DAZSNG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.209"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..192
                     /locus_tag="ACHW4Z_004056"
     CDS             <1..192
                     /locus_tag="ACHW4Z_004056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883445.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHW4Z_004057"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHW4Z_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR8883446.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHW4Z_004058"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHW4Z_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR8883447.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHW4Z_004059"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHW4Z_004059"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR8883448.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1035 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAZSNG010000206         4182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZSNG010000206 DAZSNG010000000
VERSION     DAZSNG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.210"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..186)
                     /locus_tag="ACHW4Z_004060"
     CDS             complement(<1..186)
                     /locus_tag="ACHW4Z_004060"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR8883449.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHW4Z_004061"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHW4Z_004061"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR8883450.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHW4Z_004062"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHW4Z_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR8883451.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHW4Z_004063"
     CDS             3863..4144
                     /locus_tag="ACHW4Z_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR8883452.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZSNG010000207         4169 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZSNG010000207 DAZSNG010000000
VERSION     DAZSNG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.211"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHW4Z_004064"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHW4Z_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR8883453.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQARCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHW4Z_004065"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHW4Z_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHW4Z_004066"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHW4Z_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4169
                     /locus_tag="ACHW4Z_004067"
     CDS             3787..>4169
                     /locus_tag="ACHW4Z_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR8883454.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1039 a         1209 c         1020 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agcttgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctacatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggca
//
LOCUS       DAZSNG010000208         4123 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZSNG010000208 DAZSNG010000000
VERSION     DAZSNG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.212"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            238..3546
                     /gene="icsA"
                     /locus_tag="ACHW4Z_004068"
                     /gene_synonym="virG"
     CDS             238..3546
                     /gene="icsA"
                     /locus_tag="ACHW4Z_004068"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR8883455.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3690..4008
                     /locus_tag="ACHW4Z_004069"
                     /pseudo
     CDS             3690..4008
                     /locus_tag="ACHW4Z_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1240 a          716 c          971 g         1196 t
ORIGIN      
        1 ggtctgaagc agactatcat agtaaaatta ttaatttatt atcgaacata tagctttccc
       61 cctctttttt tcaaagcaag acacaggtaa atttctcccg ttgcattgat atataacaca
      121 gctctcatgt tttggttgag gctttgttta atatgttttt gcatatatcg tccctttatt
      181 ccggataaaa cggaatcttt tcaggggttt atcaaccact tactgataat atagtgcatg
      241 aatcaaattc acaaattttt ttgtaatatg acccaatgtt cacagggggg ggccggagaa
      301 ttacctacgg taaaggaaaa aacatgcaaa ttgtcttttt ctccttttgt tgttggtgca
      361 tccctgttgc tcggggggcc aatagctttt gctactcctc tttcgggtac tcaagaactt
      421 catttttcag aggacaatta tgaaaaatta ttaacacctg ttgatggact ttctcccttg
      481 ggagctggtg aagatggaat ggatgcgtgg tatataactt cttccaaccc ctctcatgca
      541 tctagaacta agctacggat taactctgat attatgatta gcgcaggtca tggtggtgct
      601 ggtgataata atgatggtaa tagttgtggc ggtaatggtg gtgactctat taccggatct
      661 gacttgtcta taatcaatca aggcatgatt cttggtggta gcggcggtag cggtgctgac
      721 cataacggtg atggtggtga ggctgttaca ggagacaatc tgtttataat aaatggagaa
      781 attatttcag gtggacatgg tggcgatagt tatagtgata gtgatggggg gaatggaggt
      841 gatgccgtca caggagtcaa tttacccata atcaacaaag ggactatttc cggtggtaat
      901 ggaggtaaca attatggtga gggtgatggc ggtaatggag gtgatgccat cacaggaagc
      961 agcctctctg taatcaataa gggcacgttc gctggaggca acggaggtgc tgcttacggt
     1021 tatggttatg atggctacgg tggtaatgct atcacaggag ataacctgtc tgtaatcaac
     1081 aatggagcta ttttaggcgg taatggtgga cattgggggg atgctataaa tggtagcaat
     1141 atgaccattg ctaatagcgg atatataatt tcaggtaaag aagatgatgg aacacaaaat
     1201 gtagcaggta atgctatcca catcactggt ggaaacaatt cattaatact ccatgaaggt
     1261 tctgtcatta ctggtgatgt acaggttaac aattcatcca ttctgaaaat tatcaacaat
     1321 gattacactg ggaccacacc aactattgaa ggtgatttat gtgctggtga ttgtacaact
     1381 gtttcactat caggtaacaa attcactgtt tcaggtgacg tttcttttgg tgagaacagt
     1441 tctttaaatt tagctggaat cagtagtctg gaagcttctg gaaatatgtc atttggcaac
     1501 aatgtaaaag tggaggctat tataaataac tgggcgcaga aggactataa actgctaagt
     1561 gcagataaag ggataacagg tttcagtgtt tctaatatat ctatcatcaa tccgttactc
     1621 actactggtg ctattgacta tacaaaaagc tatatcagtg accagaataa attgatctac
     1681 ggtttgagct ggaatgatac agatggcgac agtcatggag agttcaatct gaaagaaaac
     1741 gctgaactta ctgttagtac tattctggca gataatctca gccatcataa tataaatagc
     1801 tgggacggaa aatccctaac aaaatcaggg gagggaactc tcattttggc ggaaaaaaat
     1861 acctactctg gtttcaccaa catcaatgca ggcattctaa aaatggggac agttgaagct
     1921 atgacacgta ccgctggtgt tattgttaat aaaggtgcta ccttgaattt ttcaggcatg
     1981 aaccaaactg ttaacacttt attaaatagt gggactgtgc taatcaataa tattaatgcc
     2041 ccttttttgc ctgaccccgt cattgtcaca ggtaacatga ctctggagaa aaacggtcat
     2101 gttattctca ataatagttc gtcaaatgtc ggtcagacct atgttcagaa aggtaattgg
     2161 catggaaagg gcggaatatt atctttgggc gcggttctcg gcaatgacaa cagtaaaact
     2221 gaccggctgg aaattgcagg ccatgcgtct ggtattacct atgttgcagt gacaaatgag
     2281 ggaggctctg gagataaaac tcttgaaggt gttcaaatta tttcgacaga ttcttctgat
     2341 aagaatgctt ttattcagaa aggccgtatt gttgctggta gttatgacta tcgcctgaaa
     2401 cagggcactg tatctggact gaataccaat aagtggtatc taactagtca gatggataat
     2461 caagaatcaa aacagatgag caatcaagag tctactcaaa tgagtagtcg ccgagctagt
     2521 tcacagcttg tatcttcact taatttgggt gaaggtagta ttcacacatg gcgccctgaa
     2581 gctggcagtt atattgctaa cctgatagca atgaacacga tgtttagtcc ttctctctat
     2641 gaccgacacg gtagcactat tgttgatcct actacaggtc agctcagcga aaccaccatg
     2701 tggattcgta ctgttggtgg acataatgag cataatttag ctgatagaca attaaaaacc
     2761 acagctaaca ggatggttta tcagattggt ggagatattt tgaagacaaa cttcactgat
     2821 catgatggct tgcatgtggg tattatggga gcttatggat atcaggatag caaaactcat
     2881 aataagtata ctagttatag ttcacgagga actgtgagcg gttatactgc cggtttgtac
     2941 agttcttggt ttcaggatga aaaagaacga acaggtctat atatggatgc ttggttgcag
     3001 tacagttggt ttaataatac agtcaaagga gatgggttaa ctggtgagaa atattccagc
     3061 aaaggaataa caggagcttt ggaagctggc tatatctacc caaccatacg ctggactgct
     3121 cataataata ttgacaacgc attgtatctc aatccacaag tccagataac taggcatggg
     3181 gtaaaagcaa acgactatat tgaacacaat ggcactatgg tcacatcctc tgggggcaat
     3241 aatattcaag caaaattggg attgcgtaca tccttaatta gtcagagttg tatcgataag
     3301 gagactcttc gtaagttcga accatttttg gaagtgaatt ggaaatggag ctcaaagcaa
     3361 tatggtgtaa ttatgaatgg catgtcaaat caccagatag gcaaccgtaa tgtgattgaa
     3421 ctcaaaactg gtgtgggggg gcgtcttgca gataacctaa gcatctgggg aaacgtatct
     3481 cagcaattgg gtaataacag ttacagagac acccaaggta ttttgggtgt gaaatatacc
     3541 ttctgataat aaaaatgcag ggcgtgttga tgtcctgcat ttctctggga cccaccgtgt
     3601 aaaagctcgg tgggtttttg tgcacatgat ggtatgcctg cgatcaatgc caaacgtgtt
     3661 taccagatca tgcgccagaa tgcgctgttg cttggcgtta cacaggaatt gcattagaaa
     3721 gcgatgaacg aatgctaccg ccaaatattc gtggttatgc gcctcagatt actggtattg
     3781 cagaaactaa tgcacgtgtt gttgtatctc aacaaggtcg agtaatatat gactctactg
     3841 taccagctgg aactttttct attcaggact tgagtagttc tgtacgagga atattggatg
     3901 ttgaaatttt tgagcaaaat ggtaaaagaa aacatttcca agtagaaatg tgcaggtgtg
     3961 cctttcttat ccagacttgg tcagagtagg tataaattag taacaggtct acctaaaaca
     4021 aataataaag caactggtga tgcatttttt tcggtgaaga tcctacgggg tcctgagccc
     4081 ggtgaagtgg cgaggcaaat cacatgggga agcgtacagc ctg
//
LOCUS       DAZSNG010000209         4042 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZSNG010000209 DAZSNG010000000
VERSION     DAZSNG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.213"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(126..284)
                     /locus_tag="ACHW4Z_004070"
     CDS             complement(126..284)
                     /locus_tag="ACHW4Z_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8883456.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHW4Z_004071"
     CDS             complement(886..1077)
                     /locus_tag="ACHW4Z_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8883457.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHW4Z_004072"
     CDS             1142..1465
                     /locus_tag="ACHW4Z_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR8883458.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHW4Z_004073"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHW4Z_004073"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHW4Z_004074"
     CDS             2143..2673
                     /locus_tag="ACHW4Z_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR8883459.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHW4Z_004075"
     CDS             2680..2913
                     /locus_tag="ACHW4Z_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR8883460.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHW4Z_004076"
     CDS             complement(3459..3851)
                     /locus_tag="ACHW4Z_004076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883461.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZSNG010000210         3968 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZSNG010000210 DAZSNG010000000
VERSION     DAZSNG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.214"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            492..1283
                     /locus_tag="ACHW4Z_004077"
     CDS             492..1283
                     /locus_tag="ACHW4Z_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883462.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1669..1875
                     /locus_tag="ACHW4Z_004078"
     CDS             1669..1875
                     /locus_tag="ACHW4Z_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8883463.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1961..2533
                     /locus_tag="ACHW4Z_004079"
     CDS             1961..2533
                     /locus_tag="ACHW4Z_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883464.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2535..2819)
                     /locus_tag="ACHW4Z_004080"
     CDS             complement(2535..2819)
                     /locus_tag="ACHW4Z_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883465.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2790..2933
                     /locus_tag="ACHW4Z_004081"
                     /pseudo
     CDS             2790..2933
                     /locus_tag="ACHW4Z_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3788..>3968)
                     /locus_tag="ACHW4Z_004082"
     CDS             complement(3788..>3968)
                     /locus_tag="ACHW4Z_004082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883466.1"
                     /translation="QRYIDVFFMVKNHIFFYIHNVMIVCSAFWGCQRRTGCADHMRRM
                     RCPGDSPPQYSVFSF"
BASE COUNT         1210 a          916 c          767 g         1075 t
ORIGIN      
        1 gttgcgggac tacattacag cagagagggg taaggacaga gttacaagta ctactagaga
       61 gaatatcact gcacagatac ttctatgagt atatctttat gagagatccg tctacaggat
      121 tgggttataa ggtgtattta caaggtataa tcacaggatc atttacatga ccatacttct
      181 gtaaataata ctaattaacc ttaattattt ttttatggta catgaactac ctgtagctgt
      241 aacgataaac aaatcaccca aaatgaaaaa tactggttat cacagttcag agaactcgta
      301 tgagtgtccc cctgagcaat gcttcatttt ctgtgattct gtgtgataga gttatactga
      361 gtacagtggc agtaacactg ctttccatca cttgaggata aaattcatta aaaaattaat
      421 tgcatgaatt aacaggtttt tttatgaaat gttatgcatc tcaaccaata tgattaacag
      481 aaaacttcat catgacaaca cgtataacgt tcagtatgac cagtgatgag acgctgcgaa
      541 ttgtggatga gtactgtcat acccacaaac tttcgcgctc taaggtaatc aacgcattac
      601 tcagcgccac agcacctgtc cttaatgata ttaactgtta ttatcaactt gcaggcgaac
      661 tgcagtcacg cttattaaat ggagtgtatc aacgggatct tcctcgcaaa cgcaatgtcg
      721 tcagtgcaga gaagtattgt ctggaaatct gggaaaacaa acttttcacc agaagaatcc
      781 tggagtttga cagttcaaat ggtgttctgt acgcgctgaa acataaaaga cactatcgcc
      841 gggataagat gattggaagg gttgaaagtc ggtgtatcaa agacatttgc gaataccaga
      901 tgcagctatc cggggagaag acgaaatatg cctgttttat ttatatcgaa agaacaatat
      961 acaatcacga taatccatct gataaaatac ctgtaaaagc ggctgttggt aatgcagtca
     1021 tcctgctggc aaaggatgtg atatataacg agtacttttt tgatttgcgg cagtcatttt
     1081 ttgtgagcgt tacagatctg atggtctctg gcgcaaaagg tattccggag acacagacat
     1141 atccggacgt ttattgctgg atccctcttt tttccataaa ttccggcgtg ctcattaccc
     1201 ctgtatataa aattgaccct ctgaaacccg taacggtaaa aaaaccggat aagatcactg
     1261 ttgtttgtaa ttacagagaa taatttcagc cttctacctt caccgcgaca gtgtttacct
     1321 ctgctttatc ttccgttcag taaatacaaa ccaccgctct ggcaggtaca gggcgtggtt
     1381 tatctccgat actcccctgt gcagtaataa tcatcactga aaatactgat cccgtcacgg
     1441 caaaacgcca tcccatattc cggcaacaca aaatcatctt ccgtttatac cggattcact
     1501 gacaaaacag acaacctgta ttgcctgtac acaacagttt ctctcctggc aggaatattc
     1561 ccctactcat tttttacgtt tctccccggt tgttatgccg gtgaaggtgg tgcgtcgttt
     1621 tcatcaccaa cgccggttga gattaacaac atccggagga acattctcat gaatacccca
     1681 gtttcactga tggatgacca gatggtcgac atgtcgttta tcactcagct gaccggcctg
     1741 accgataagt ggttttataa actcatcaag catggagcct ttccgccccc catcaaactg
     1801 ggccgtagct cccgctggct gaaaagcgaa gtggaagcct ggctgcaggc gcgtattgca
     1861 cagtcccgtc cgtaattcct gaccgttatc cgttccaccg cagcaaaggc atgatccggc
     1921 ccccggaggc attttgctgc gcgctaaagg agagaatatt atgcaacaac ttgaaaacag
     1981 gtgtgacctg ctgctcatcc agcatcagaa atggatgaca agcgtgacga ggcttatcgt
     2041 cgctcatgga atggggtctc cgcatcttca tggttatcac cgtctgaccc tggcacactt
     2101 ttttctgccg gaaaaaggct ccgtcatttc ggtggccccc cagggactct atcaggtcgt
     2161 caacccggga acaccaccgt tcattcctgc cattcaggaa gggttaatga ccagcattca
     2221 gacccatgaa atcatgctgc ttacacattt taatctgggc ggcgttttac tcagtgagct
     2281 acatcgtctg ggtgaaaacc ggctggcaaa caggctgaac agcctgctcc ggcgtttcga
     2341 cgatcgtgat ctctaccaca cgctgatatg gctgtgctgg tatgatctga tgtgtgccca
     2401 ctccatgcag ccctggactg aagagttaaa gcataaatca catgcagagc ttgaaaactg
     2461 ggctgtggcg cgcaaacggg aaaagcgcga gctggaactg atgattgacg agtacctgtt
     2521 atacgcctgc tgattcataa cgttcccggg gttccttcct catcgttaca cacagccgga
     2581 cggctatcta tggctgtccg ccaaatgacc atcgccgcat ttcgttccgc tgaaaacggt
     2641 acgtagtcag tgataccgga atttccggag agggccgcag gtatattgct tctgtgcaat
     2701 tgatgcttat ttacattgga tgtagttatt tctgcatcca atgctatcgg aaagagatta
     2761 accagcaaat ggttcctcaa atcgtattga tatttacccg gcgtattgag caccaccatc
     2821 tgacactccg ttcccgcatc aaacgactgg accgaaaaac gatttacttt tcacgtgctg
     2881 tggaaattca cgaaaaaatt attgggtcat tcatcgaaaa acatatgttc tgatgactgg
     2941 agtcatcctc cagaaatata cgtcaactga acaatatcga ttttttatgg gattctatac
     3001 acttttccat atccctctga aaatcagtca tcagctcacc aacacctctt ctgaaacttg
     3061 tattaaactt attgcttaaa tagatgatat aacatctctt ccattgcact gaatcggcga
     3121 aaactgccgt ttcaataatc tcaacaatat tatttatcgg ctaactttat cagtaaacaa
     3181 tggaatgtca cactgatgaa atgaacattc agagtaagat gataaaattc atataaacgc
     3241 cattgctgaa tattatttat ttcatacaga taaacgataa aatacaaaag ggtatatttc
     3301 tcaatgtaac agtcaattat tcatctccac tggcgagcac ggtcagcaca caaacaaagt
     3361 tagtcctcct gcttaatcat tgcgtatcag aaactaaaaa agaaaaaatt ctgcagacaa
     3421 cttaacaatc ttcatcacat ttcctgaaga cagacatatc cacaaacggt agtcagcgtt
     3481 gaattaacaa gtcttacaga gctatacgta gcttataaag ccactcaatg ttcatcatca
     3541 cccgactact tatctggaat accaccagta taaactcatc aataaaaaca cctgttgtta
     3601 aaataagaat actgtcagca gtaaattatt aaaacaacat ctgtccacac tatctgttct
     3661 tttaacgcca acggcagcga aaatagtgca tcacagcctt cttttttctt tcgccaccac
     3721 tcagacaaac acaacactca cacccacacc acaaaacaga atcaaaacat aaaattaaca
     3781 ataacagtca aaaggaaaac acagaatatt gaggtggact gtcaccagga cagcgcatac
     3841 gacgcatgtg atcagcacat cccgttcttc gctggcatcc ccaaaatgca gaacaaacaa
     3901 tcataacatt atgaatataa aagaaaatat gatttttcac catgaaaaaa acatcaatgt
     3961 atctctgt
//
LOCUS       DAZSNG010000211         3922 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZSNG010000211 DAZSNG010000000
VERSION     DAZSNG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.215"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1283)
                     /locus_tag="ACHW4Z_004083"
     CDS             complement(<1..1283)
                     /locus_tag="ACHW4Z_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR8883467.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQG"
     gene            complement(1568..1900)
                     /gene="pemK-mt"
                     /locus_tag="ACHW4Z_004084"
     CDS             complement(1568..1900)
                     /gene="pemK-mt"
                     /locus_tag="ACHW4Z_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR8883468.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1902..2159)
                     /gene="pemI"
                     /locus_tag="ACHW4Z_004085"
     CDS             complement(1902..2159)
                     /gene="pemI"
                     /locus_tag="ACHW4Z_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR8883469.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2252..2905)
                     /locus_tag="ACHW4Z_004086"
     CDS             complement(2252..2905)
                     /locus_tag="ACHW4Z_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR8883470.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3844..>3922)
                     /locus_tag="ACHW4Z_004087"
     CDS             complement(3844..>3922)
                     /locus_tag="ACHW4Z_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883471.1"
                     /translation="VERRVKERMILSRNRNYSRLATASP"
BASE COUNT         1060 a          902 c          860 g         1100 t
ORIGIN      
        1 ccctgtttaa aatcataatt tggttgttct gttcttgagt tattaagtag agttcttatc
       61 agtgtttgcc aagcatgatt gataaattta ttttctgatt ctttaaaata tcgtctgatt
      121 aaagcacata aatcttcgac acttttctga cgagcgctag ctgcccaacg gcccccttcg
      181 gtttgcacca ttgaacgttg tacactgtct cctaactgag cgataaattt ctgttcatcg
      241 tcaagttgca tgttttcatt tatgattaat tcatacaaag caagaaaaac agcttgatag
      301 taacgaggac attcttgaga tgaagatgca ttgcccagaa tgtgtgatct aaagttcaaa
      361 ttatttgcat caaataattt ttgaatcatc tcataaacga agaggtaatt attgacaatt
      421 ttatctgcac catttgtctg aatgtaattc tctaattgag ttcgtgtatg gattgcatga
      481 ctcgtcgaac cttcaccgta aaatgtatcg agcgatgttg atcctgatgt tggcttatca
      541 tctaaaaata tatagcccaa gatatttgca actaactctt catcgtcaga gtctctaatg
      601 ctaggcctac taatgatatg atttctaata tagaaatgat tgtctatatt tattccataa
      661 tcgagtccat cattgcaaat tgatatgtta tgtattttat tcagtcccat tatatcgcta
      721 tgtgtagtat ctcctcttat aattgtcgat attttcctta ctaactctga aaaattacta
      781 acgcaccccg cacttcgtag ttcttgtttt gataaaattt taccattgga atttatgcgt
      841 cgaaacactt cgtcaatgtg gtcctctgtt gaagagtatt cagatatagc aaatctatag
      901 cgagcaatta atgtagaact ttcgcgggat agcactggct ctttttgttg aagtttacca
      961 ctgtctttta gttgttttgt taatgcaatg gcatctaaat taaagtattt accatttaat
     1021 gaaaatcgct gctcaataaa actcattaaa gcttctagtc tttgcattcc atcaaggatt
     1081 tctcttttgt tgtcaatgga aacaaacaat agcaaaggga tagggtattg ctgagtcatt
     1141 gatgaaatca gtgctgtttt ctcttcgagg gaccatacta attttctttg gtatcttcga
     1201 tttataatta atttatcatc cagataccac tcgtataatt cttctgctgt cttggagtca
     1261 atatctaagt tttcattagc cattagatgt atctcgagta tttagttaag aaactgatag
     1321 gtcagtattt gcatcaatgt tcattgaatt tgtatccgaa gtcaatctga tgtaggaact
     1381 tcaaaactgc gcaaaacacg caaatacgca caagaaagta gggctgcgct tggaattcgg
     1441 aaaataaacc acatcaaaac tactttttag ccaaataacg tttgggaatc accagaatgg
     1501 tgggacaaca gcggttttag tgccctaaat cgtacgtttt catccagttg cccctcaaac
     1561 cccatgttca agtcagaata gtggacaggc ggccaagaac ttcgttcatg atagtctccg
     1621 gaacccgttc gagtcgtttt ccgccccgtg ctttcatatc aattgtccgg ggttgatcgc
     1681 aacgtacaac acctgtggta cgtatgccaa caccatccaa cgacaccgca aagccggcag
     1741 tgcgggcaaa attgcctccg ctggttacgg gcacaacaac aggcaggcgg gtcacgcgat
     1801 taaaggccgc cggtgtgaca atcagcaccg gccgcgttcc ctgctgctca tgacctgcgg
     1861 taggatcaag cgagacaagc cagatttccc ctctttccat gtcagatttc ctcctgacca
     1921 gtcgccggtg catccagcca ttctcgttct tcagctgata tttcagcatt cggatcacac
     1981 tgtgccagta gctcagccag tgaatattgc gggcgtctgt acggctcaac aatcagccgg
     2041 ccattatcaa tgaccatgcc aacttcatta tctgtgccca gagacagcgc attcagcagt
     2101 gccggtggga cggtcagcat aactgagccg ccaaccctct tcagtcgggt ggtatgcatt
     2161 cttcacctcc ataaaagtta tatttaaata taacatccac taaaaaaaca caccaggctt
     2221 taacgcacaa tgtttaataa aaatataact ttcaaccaaa cagtaaaccc agcgtggttg
     2281 cttccatatg cagcaatacc ggcagcagca ggccaccact tctgatcctg gccactgatg
     2341 taatcaaccc caccaggaac agttctgcca gtgtcagcag gttctgatac tggctgtgcg
     2401 cggcgacgaa caacaacgac gttatcagcg cccccagcca catcgtccag cagtaccgtg
     2461 aacggaagac gttcagcata atcccccgga acagcgtttc ctcattcaac ggggcaagga
     2521 taaagatggt cagcaacgtc aggatcacgt caggtatgga cttatcggca aaaagtttcg
     2581 tcataaatgg ctcagcaggc agagccagcg ccttaccgag cagaaataca ccgacataca
     2641 ccacggccat cgcaccgacc agccacggta cgccaacgtt gcgcagctga ccaacgaccg
     2701 gtagcggagc tatccaacgg cggtatacca ggaaaacaca cagcaggtac atcagaacag
     2761 taccatgact gaagaacaaa tagttttttc ctgatccata aagcagaacg gcctgctcca
     2821 tgacaaatct ggctccccaa ctaatgcccc aggcagccag cataaccagc ataaactgca
     2881 gatattgatt acgtgtttga atcattgcat cgcctgtaaa tttttaactt gtcctatttt
     2941 tgtcattacc acgtatatac acatgtataa caattcagat atcgttacca ggatatgccg
     3001 catcagcggc atggaaggcg gcactctgtt gtttcatatg atacaggagt aaaaccgccg
     3061 aagcccggcg taagccggta ctgattgata gatttcacct tacccatccc cagccctgcc
     3121 agaccatacc cgctttcagc catgagagag cttctgtgcg cggtcggagt ggtcccgacg
     3181 agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg gccgtgaggg ccgcttacga
     3241 gcgtgtactg agaacttcca gcgagaagac tgacagcgat gaagatgtag ttacaacatt
     3301 cataattaaa agcgactctg ttccggccct ttgggccggg gcggggccgc ttttcagtta
     3361 tgagggaggg gctttgtggt ttcggttctg cgctggaccg gggtttttct ggaggttgtt
     3421 tttgtgtgtt gtaactaaag tggctccggt cggggcccgc cgcttgcggt gggaggtgca
     3481 tatctgtctg tccacaggac aggcagtgaa taggttttct ttttaaatga atgtaattaa
     3541 gtagtttaaa ggagatataa acaggtgttt aaaagataca ttgcaccctg taagactggc
     3601 ggctggcgct ttatgacatg aacggttgta accttatggg gaagtccctt gcagttaaat
     3661 gtggataagc aaaattcccc gtcgctgagg cgtattttgt attaaaaaca gggggaatcg
     3721 gatgctccag aaggtggatg atgagattgt tttttgcatg cgacgctgtt tttttgtgca
     3781 ccggcgggct tcaggcgtgc gaatgcctcc ggcgcgggcc ggattattca gaggaggtca
     3841 ctttcagggg gaagctgtgg ccagccggct gtaattacgg ttacgtgaca gaatcatgcg
     3901 ctccttcaca cgacgctcaa ct
//
LOCUS       DAZSNG010000212         3757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZSNG010000212 DAZSNG010000000
VERSION     DAZSNG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.216"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..757)
                     /gene="repA"
                     /locus_tag="ACHW4Z_004088"
     CDS             complement(<1..757)
                     /gene="repA"
                     /locus_tag="ACHW4Z_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8883472.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTA"
     gene            complement(750..824)
                     /gene="tap"
                     /locus_tag="ACHW4Z_004089"
     CDS             complement(750..824)
                     /gene="tap"
                     /locus_tag="ACHW4Z_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8883473.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(1069..1317)
                     /locus_tag="ACHW4Z_004090"
     CDS             complement(1069..1317)
                     /locus_tag="ACHW4Z_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8883474.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(1602..1751)
                     /locus_tag="ACHW4Z_004091"
     CDS             complement(1602..1751)
                     /locus_tag="ACHW4Z_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8883475.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(1998..2471)
                     /locus_tag="ACHW4Z_004092"
     CDS             complement(1998..2471)
                     /locus_tag="ACHW4Z_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883476.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(2626..3216)
                     /locus_tag="ACHW4Z_004093"
     CDS             complement(2626..3216)
                     /locus_tag="ACHW4Z_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8883477.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(3254..3463)
                     /gene="hha"
                     /locus_tag="ACHW4Z_004094"
     CDS             complement(3254..3463)
                     /gene="hha"
                     /locus_tag="ACHW4Z_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR8883478.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(3509..>3757)
                     /locus_tag="ACHW4Z_004095"
     CDS             complement(3509..>3757)
                     /locus_tag="ACHW4Z_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8883479.1"
                     /translation="AGKTVTVTYFQRDRYGRILGQVYAPDGMNVNQFMVRAGAAWVYE
                     QYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT          933 a          991 c          811 g         1022 t
ORIGIN      
        1 tggcagtgaa gcgcccttcc gcgatttcgc gcgtcagctg ccgtttcacc agggtgacaa
       61 tatcctgacg ttccctgtcc gcatcacgac gcgcacgggc acgctttatt ccccgggact
      121 taagctctgt ctgataactg cgaaaacgct cacgaacaaa acgccaggct ttcgctatca
      181 gttcatccat gcccagggta tccagcccct gctttttgcg ttgtttgttt tcccatacca
      241 cacggctgcg gcgcgcggcg gccgctgcct cctctgatac atcgagggca gcaaacagtg
      301 cagatgtgaa cgtgatatcg gtcggaatgt agcacccgat aagcgggtca tattccgtct
      361 ggtaggtaat cagtcccagc tctgacagga acgtcagggc ccgggtggca cgggtgatgg
      421 agagttttcc ggcagcagac tccgtcgcca gtccgcactc aatggccagc gtggtgatgg
      481 agcactggac gcggttggcc agcgggtcat agtggaaaca cagcccctgc aggagcgcat
      541 caatagcccg tcgacgcagc accggtggca tgcgtcgacg cagaccacgc gaacgggcat
      601 gcgccacatg aatggcgaaa tcaaaacggg aagtgaagcc caccgccttt tccatcagtt
      661 tttcgcagaa cttcagcgtt ccggcacctt tacggggtgt aaacaccgga ttcgggttct
      721 ttacctggcg gtaatacgtt tgttgaagat cagtcacacc atcctgcact tacaatgcgc
      781 agaaggagtg agcacagaaa gaagtcttga acttttccgg gcatataact atactccccg
      841 catagctgaa ttgttggcta tacggttttt agatggcccc ggtaatcttc tcagtcgcca
      901 aacttgtcga agattatcgg ggttttcact tttctggctc ctgtaaatcc acatcagaac
      961 cagttccctg ccaccttacg gcgtggccag ccacaaaatt ccttaaacga tcagcaatct
     1021 atcacgcaca ccagaaacag gcaagaatgt gatgtcgcta cattttagtt agctaaaagc
     1081 actcttctgc tttatcagtt cggcaatcat ctcagcctgg gtaacgccct cagcttcaca
     1141 catcacctgc aattgcgctt ttaacgcagc aggaatgaac actctgagtt ctttatgtgt
     1201 agctcgtttt cttgccaggg atgcctgctg acgttctgca agcgtcagag gattaccttt
     1261 tctgtatgct cgctttgtac ctgatgagga agtcactgca ttttctgtct gcgacatctc
     1321 gcctcctcaa tactcaaaca gggatcgttt cgcagaggat actacagttt tttgaaatca
     1381 gcgacttgag aattgtgacg aagatctgtg ttggcgcaca aatcaacggg gattactgtt
     1441 gtttaatgtg atttttgttg cgaaatggct gaaaaaacag gaaatgcctt aacccgggag
     1501 ccggccccgg gcgggaccgg ctgtttctgg catgtcgggt gtctgcccct gaaatacctc
     1561 acacttccgg aaagcgggga cttccgtccc cgcccggcag tttacttccg tgcttcgtag
     1621 gccagagtta cctgcaccac tgtatttccc ctgtgaatat tcagctcaca taaccgttcc
     1681 ctgaatatca gtgaaaaaca caacaccgtg gcgcacacgg cgagcaaccc gataagggca
     1741 tacttcgtca taaactttga cctctctgca aggaagaggc tacaatcagt agtgcaatgt
     1801 acttcatgca gacctcacga gttaatggat taacaagtgg ggtctttgca tttctggtac
     1861 ctgcgtcttg cctgaaccag aaaggctcag acacccgccg cgataatacc acattccctg
     1921 ttgctgacta ttacctgcat tgcccgcttt ttactacaga agcacgaagg cgtcactcca
     1981 gccaataagt atgcttttta tttacatcca tttttaatcc ggtaccgacg ttttcctcca
     2041 gttaactcgt ttttcgtatg actcatggca ataaaatttt ctttaccata tgacaacatc
     2101 ccaataatat caaagttgtc ttttaatttg atggcatcct tttcacttac agcgacaata
     2161 agttgtttac caaattttct gaccatcatt gcaaactgtg atactgctga tccaccacct
     2221 aaatcaggcc acccagcaca attaaaaatg attgtctgct cttcactatt cattgcatcc
     2281 ctgataaagg agacagcatc ctcatttctc tctacatctg aatcactgtt aatctgagtg
     2341 aaatcataat atttagaacc tggtatagaa taccccttaa aaaacttatc aatgtgggag
     2401 gcagagttcc caacaataag aatattgtgt tcacgcttaa tcatcaattc tgatacatca
     2461 tcatatttca tatattcacc attattatat aaagtttggg tacaggtggt tccggaattt
     2521 actctgtgac caccatttcc cgctttttat tacagaaacg ccgggagcgg ggaaaacttg
     2581 ctttttcccg tttccggggt tggacaactg agcaacgcga aggcgtcagc tacgatgttc
     2641 cggggactgc tgatcagccc ctgttgtatt cagccattct ccggtcatct gcagcagttt
     2701 tctggcatcg tggcttctga gcaacggaat accagcctgc ctcagaacct cttccagaag
     2761 aatatcccgg cgccggcgtt ccggtcgtaa atggctggca tcatccagct ctactgctgc
     2821 aacaatactg aacgaacgcc gttcaacgat aaccacatca acatgccact gagacaccat
     2881 cctgaataac tgccaccact ggcgtgatcg tggccggata ttcccgttca gctggacgat
     2941 atccgcgacc cgtacctgcg gacacagata ccaccgcttc atatccacaa ttctgaacag
     3001 tcctttcata aaactgactt ccctttctgt cagcagcgtc ttctgacgtt gccagtattc
     3061 accgccggac agtattcgct ctgcatcacc ggtccgtata cctgccgcat gcaactgcat
     3121 cagcaaagga gaatgctgat ttacactttt gaggataatc cggataagta acgctgcaat
     3181 aatcagaaca atgagaataa aaataatcag cttcattgtt tatctgctcc tggtaattaa
     3241 ttattctgaa ttattacctt acatatttcc atatctcttt tggtatcctg tcatataact
     3301 ttccggtgat taattccgct aaccggtggt ctgctgctga attaaatgat tcacgttcac
     3361 tgtttaataa actgtctctg tttttatgaa taattttttc cagcgtattg actgatgaac
     3421 atcgccgtaa ctgatacagc cattcctgtt ttgttttcgc catttttatt cccgctgaga
     3481 acagatgaat gataagattg ccatcgcttt atttacggtg tctccatatc caggggggaa
     3541 ccggatcagc atctgaccag agcccacgtt tttgttgtct ggcttcgttc tgaaggacag
     3601 gcagcacggg atctgtgttg tactgctcat atacccaggc agccccggcc cggaccataa
     3661 actgattaac attcattccg tctggtgcat aaacctgccc gagaatacga ccataacgat
     3721 ctcgctgaaa atatgtgacg gtaaccgttt ttcctgc
//
LOCUS       DAZSNG010000213         3737 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZSNG010000213 DAZSNG010000000
VERSION     DAZSNG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.217"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            368..2020
                     /gene="ushA"
                     /locus_tag="ACHW4Z_004096"
     CDS             368..2020
                     /gene="ushA"
                     /locus_tag="ACHW4Z_004096"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFR8883480.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="ACHW4Z_004097"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="ACHW4Z_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="ACHW4Z_004098"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="ACHW4Z_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..3526)
                     /locus_tag="ACHW4Z_004099"
                     /pseudo
     CDS             complement(2527..3526)
                     /locus_tag="ACHW4Z_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1032 a          861 c          857 g          987 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggaacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg tatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca tcatcggcgt
     2941 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3001 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3061 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3121 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     3181 catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     3241 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     3301 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     3361 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     3421 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     3481 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     3541 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     3601 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     3661 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     3721 tttactccgt ccctgta
//
LOCUS       DAZSNG010000214         3715 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZSNG010000214 DAZSNG010000000
VERSION     DAZSNG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.218"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            18..419
                     /gene="spa15"
                     /locus_tag="ACHW4Z_004100"
                     /gene_synonym="spaK"
     CDS             18..419
                     /gene="spa15"
                     /locus_tag="ACHW4Z_004100"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR8883481.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            423..1715
                     /gene="spaL"
                     /locus_tag="ACHW4Z_004101"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             423..1715
                     /gene="spaL"
                     /locus_tag="ACHW4Z_004101"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR8883482.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1796..2146
                     /gene="spa13"
                     /locus_tag="ACHW4Z_004102"
                     /gene_synonym="spaM"
     CDS             1796..2146
                     /gene="spa13"
                     /locus_tag="ACHW4Z_004102"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR8883483.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            2133..3011
                     /gene="spa32"
                     /locus_tag="ACHW4Z_004103"
     CDS             2133..3011
                     /gene="spa32"
                     /locus_tag="ACHW4Z_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR8883484.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            3005..>3715
                     /gene="spa33"
                     /locus_tag="ACHW4Z_004104"
                     /gene_synonym="spaO"
     CDS             3005..>3715
                     /gene="spa33"
                     /locus_tag="ACHW4Z_004104"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR8883485.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNM"
BASE COUNT         1245 a          529 c          750 g         1191 t
ORIGIN      
        1 tttagtgagg tttaaatatg agtaacatta atttagttca attagttaga gatagtcttt
       61 tcacgattgg ttgtcctcca tcaattatta cagatttaga cagccattca gcgattacca
      121 tttctttgga tagtatgcct gctattaata tagctcttgt caatgagcag gttatgttat
      181 gggctaattt tgatgcacca agtgatgtaa aacttcagtc atctgcttat aacatattaa
      241 atttaatgtt aatgaacttt agttatagca ttaatgagct ggtagagctt catcgttctg
      301 atgagtactt acagttaaga gttgttatta aagatgacta cgttcacgat gggattgttt
      361 ttgcagagat tctgcatgag ttttatcaga ggatggaaat cttaaatggg gtcttataat
      421 caatgagcta tacaaaattg ctcactcaat tatcttttcc taatagaatc tcggggccaa
      481 tcttggaaac aagtcttagc gatgtttcga ttggtgagat ttgtaacatt caggctggaa
      541 ttgaaagtaa tgaaattgtt gcaagagctc aggttgtagg atttcatgat gaaaaaacaa
      601 tattaagctt gattggaaat tctcgtggac tttcacggca aacgttgatt aagcccactg
      661 cccagtttct tcatacgcaa gtcggccgtg gattattggg agcagtagtc aatcctttag
      721 gggaggttac tgataagttt gctgttacag ataacagtga aattctttat cgacctgtag
      781 ataatgctcc tccgctatat agtgaaaggg ctgcaattga gaagcctttt ttaacaggta
      841 ttaaggttat tgattcttta ctcacgtgtg gtgaaggaca gcgaatgggg atttttgcgt
      901 cagctggttg tggcaaaact tttctcatga atatgctcat tgaacatagt ggtgctgata
      961 tatatgttat tgggttaatt ggtgagcgag gtcgagaggt tactgaaacg gttgattatt
     1021 tgaaaaactc tgagaaaaaa agcaggtgtg ttttagtata tgcaacttcg gattactctt
     1081 cggttgatcg ttgtaatgct gcatatatag ccactgctat agccgaattt tttaggactg
     1141 aaggacataa agtagcgctt tttattgatt cattaacaag gtatgccaga gcattacgtg
     1201 atgtggcctt agccgctgga gaatcacctg ccagaagagg ctatccggtt tcggtttttg
     1261 atagcttacc cagacttctt gaaaggccag gaaagttaaa ggcaggtggc tctattactg
     1321 cattttacac tgttcttttg gaggatgatg attttgctga tccattagct gaagaggtaa
     1381 gatccatttt agatggacat atatatttga gcagaaatct agcccaaaaa ggacaatttc
     1441 ctgcaattga ttccttaaaa agtataagca gggtatttac acaggttgtt gatgaaaaac
     1501 atcgtattat ggccgctgca tttcgggagc tactttctga aatagaagag ctaaggacaa
     1561 ttattgactt tggtgaatac aaaccggggg agaatgcctc tcaggataaa atctacaaca
     1621 aaatatctgt tgttgagagt tttctgaagc aagattatcg actcggtttt acttatgagc
     1681 agacaatgga gcttattggt gaaacaatta gataaggttt taaaaatcaa agataaatat
     1741 caacggtctg ttaaattaat tgaagcacac atattaacac taaaaaaaaa actctttgta
     1801 tcgagatgtg gaggcattag ataaaaggat tatatacttc ctgcaactgg agaatgactt
     1861 agaacctgta ggcgctcaga gtgttagtca gcttttcaat acaagacgta agatcgctat
     1921 agtaaaaaag catattatcc aatatcagtc tgaacgaatt cttctaaagg gaagaattga
     1981 agaaattcaa aaagatattg atgaggcaaa tgcttctaaa agaaaactgc ttcataaaga
     2041 gagtaaaata tgtaaacgga ttggcttgat taaaagaaat aactttgcca aacagttgat
     2101 acttgatgag ttatcacaag aggatatgaa gtatggcatt agataatata aacctaaatt
     2161 ttagtagtga caaacagata gaaaaatgtg agaaactatc ttcaatagat aatattgata
     2221 gtctcgtttt gaagaagaag aggaaggttg aaattcctga gtactcttta attgcatcta
     2281 actatttcac tattgataag cactttgaac ataagcatga taaaggagaa atttatagtg
     2341 gcattaaaaa tgcgttcgaa cttagaaacg aacgagcgac atattctgat attccagaat
     2401 caatggccat taaagaaaat attttgatac cagatcaaga tatcaaagca agggaaaaaa
     2461 taaatatcgg cgatatgagg gggatctttt catataataa gagtggaaat gcagacaaga
     2521 acttcgaaag aagtcatact tcttctgtaa accctgataa tctgctagaa tctgataata
     2581 gaaatggtca aattggttta aaaaatcata gcttgtctat tgataagaat attgctgaca
     2641 tcatttcttt actaaatgga agtgttgcta aatcatttga gctgcctgta atgaataaaa
     2701 atactgcaga cataacccca tccatgtcat tgcaagaaaa atcaatagtt gaaaatgata
     2761 aaaatgtttt tcaaaaaaat agtgaaatga cttaccactt taaacagtgg ggggctggac
     2821 attctgttag tatttcagtg gagtctggtt cttttgttct aaaaccgtca gatcaatttg
     2881 taggaaataa acttgactta attttgaaac aagatgctga gggtaattac agatttgata
     2941 gcagtcaaca taataagggg aataaaaata atagtacagg atataatgaa cagagtgaag
     3001 aagaatgcta agaattaaac attttgacgc taacgaaaaa ctacagattt tatatgcaaa
     3061 gcaactctgc gagcgttttt cgattcagac attcaaaaat aaatttacag gcagtgaaag
     3121 tttagtcact cttacttccg tgtgtgggga ttgggtaatt cgtattgata cattatcttt
     3181 tttgaaaaaa aaatacgagg tattttcagg attttctaca caagaatctt tactgcattt
     3241 atcaaaatgt gtctttatag agtcgtcatc tgtattttcg attccagaac tgtctgataa
     3301 gattactttc cggatcacga atgaaatcca gtatgcaact actgggagtc atttatgctg
     3361 tttttcctct tctttaggta ttatttattt tgacaagatg ccggtattac gtaatcaagt
     3421 ttctcttgac tcattgcatc atcttttaga gttttgctta ggttcatcta atgtaaggct
     3481 ggctacttta aaaagaattc gcactggtga tataatcata gttcagaaac tttataattt
     3541 attattgtgt aatcaagtta ttattgggga ttatattgtg aatgataata atgaggcaaa
     3601 aattaatctg tcagaaagta atggtgagtc agaacacaca gaagtttctt tggcattatt
     3661 caattatgat gatatcaatg taaaagtgga ctttattctt ttagaaaaaa atatg
//
LOCUS       DAZSNG010000215         3614 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZSNG010000215 DAZSNG010000000
VERSION     DAZSNG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.219"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..1235
                     /gene="yiaY"
                     /locus_tag="ACHW4Z_004105"
     CDS             84..1235
                     /gene="yiaY"
                     /locus_tag="ACHW4Z_004105"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR8883486.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1400..2938
                     /gene="aldB"
                     /locus_tag="ACHW4Z_004106"
     CDS             1400..2938
                     /gene="aldB"
                     /locus_tag="ACHW4Z_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR8883487.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3157..3300)
                     /locus_tag="ACHW4Z_004107"
     CDS             complement(3157..3300)
                     /locus_tag="ACHW4Z_004107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883488.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3483..>3614
                     /locus_tag="ACHW4Z_004108"
     CDS             3483..>3614
                     /locus_tag="ACHW4Z_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8883489.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          905 a          860 c          944 g          905 t
ORIGIN      
        1 gataattaat aataatgatc atgttttcga atgaaaatcc tcagtaagct gcccaccctt
       61 ttttacactt tcaggagtgt gttatggcat cttcaacttt ctttattcct tctgtgaatg
      121 tcatcggcgc tgattcattg actgatgcaa tgaatatgat ggcagattat ggatttaccc
      181 gtaccttaat tgtcactgac aatatgttaa cgaaattagg tatggcgggt gatgtgcaaa
      241 aagcactgga agaacgcaat atttttagcg ttatttatga tggcacccaa cctaacccca
      301 ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga aaataattgc gatagcgtga
      361 tctccttagg cggtggttct ccacacgact gcgcaaaagg tattgcgctg gtggcagcca
      421 atggcggcga tattcgcgat tacgaaggcg ttgaccgctc tgcaaaaccg cagctgccga
      481 tgatcgccat caataccacg gcgggtacgg cctctgaaat gacccgtttc tgcatcatca
      541 ctgacgaagc gcgtcataac aaaatggcga ttgttgataa gcacgtgact ccgctgcttt
      601 ctgtcaatga ctcctctctg atgattggta tgccgaagtc actgaccgcc gcaacgggta
      661 tggatgcctt aacgcacgct atcgaagcgt atgtttctat tgccgccacg ccgatcactg
      721 acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa cctgccgtta gccgttgaag
      781 atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc ccagttcctt gccggtatgg
      841 cgttcaataa tgcttctctg ggttatgttc atgcgatggc gcaccagctg ggcggtttct
      901 acaacctgcc acacggtgta tgtaacgccg ttctgctgcc gcatgttcag gtattcaaca
      961 gcaaagtcgc cgctgcacgt ctgcgtgact gtgccgctgc aatgggcgtg aacgtgacag
     1021 gtaaaaacga cgcggaaggt gctgaagcct gcattaacgc catccgtgaa ctggcgaaga
     1081 aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa agaagaagat ttcgcggttc
     1141 tggcgactaa tgccctgaaa gatgcctgtg gctttactaa cccgatccag gcaactcacg
     1201 aagaaattgt ggtgatttat cgcgccgcaa tgtaatcatc attccaacaa cggctggcaa
     1261 attgtcagcc gctttttcaa ctatctctgt aatccttgcc cgtaaattcg tgatagctgt
     1321 cgtaaagctg ttaccgactg gcgaagattt cgccagtcac gtctaccctt gttatacctc
     1381 acaccgcaag gagacgatca tgaccaataa tcccccttca gcacagatta agcccggcga
     1441 gtatggtttc cccatcaagt taaaagcccg ctacgacaac tttattggcg gtgaatgggt
     1501 agcccctgcc gatggtgagt attaccagaa tctgacgccg gtgaccgggc agctgctgtg
     1561 cgaagtggcg tcttcgggca aacgagacat cgatctggcg ctggatgctg cgcacaaagt
     1621 gaaagataaa tgggcgcaca cctcggtgca ggatcgcgcg gcgattctgt ttaagattgc
     1681 cgatcgaatg gaacaaaacc tcgagctgtt agcgacagct gaaacctggg ataacggcaa
     1741 acccattcgc gaaaccagtg ctgcggatgt accgctggcg attgaccatt tccgctattt
     1801 cgcctcgtgt attcgggcgc aggaaggtgg gatcagtgaa gttgatagcg aaaccgtggc
     1861 ctatcatttc catgaaccgt taggcgtggt ggggcagatt atcccgtgga acttcccgct
     1921 gctgatggcg agctggaaaa tggctcccgc gctggcggcg ggcaactgtg tggtgctgaa
     1981 acccgcacgt cttaccccgc tttctgtact gctgctaatg gaaattgtcg gtgatttact
     2041 gccgccgggc gtggtgaacg tggtcaatgg cgcaggtggg gaaattggcg aatatctggc
     2101 gacctcgaaa cgcatcgcca aagtggcgtt taccggctca acggaagtgg gccaacaaat
     2161 tatgcaatac gcaacgcaaa acattattcc ggtgacgctg gagttgggcg gtaagtcgcc
     2221 aaatatcttc tttgctgatg tgatggatga agaagatgcc tttttcgata aagcgctgga
     2281 aggctttgca ctgtttgcct ttaaccaggg cgaagtttgc acctgtccga gtcgtgcctt
     2341 agtgcaggag tcaatctacg aacgctttat ggaacgcgcc atccgccgtg tcgaaagcat
     2401 tcgcagcggt aacccgctcg acagcgtgac gcaaatgggc gcgcaggttt ctcacgggca
     2461 actggaaacc atcctcaact acattgatat cggtaaaaaa gagggcgctg acgtgctcac
     2521 aggcgggcgg cgcaagctgc tggaaggtga actgaaagac ggctactacc tcgaaccgac
     2581 gattctgttt ggtcagaaca atatgcgggt gttccaggag gagatttttg gcccggtgct
     2641 ggcggtgacc accttcaaaa cgatggaaga agcgctggag ctggcgaacg atacgcaata
     2701 tggcctgggc gcgggcgtct ggagccgcaa cggtaatctg gcctataaga tggggcgcgg
     2761 catacaggct gggcgcgtgt ggaccaactg ttatcacgct tacccggcac atgctgcgtt
     2821 tggtggctac aaacaatcag gtatcggtcg cgaaacccac aagatgatgc tggagcatta
     2881 ccagcaaacc aagtgcctgc tggtgagcta ctcggataaa ccgttggggc tgttctgata
     2941 taagaagctg gtcgcattgg gtattcattg cctgatgcga cgcttacgcg tcttatcatg
     3001 cctacgggaa cctgaccgta ggccggataa ggcgtacacg ccgcatccgg cataaacaaa
     3061 gcgcacgttg ttacaattta cgttttaaac ctacgtgcgc aataacccag actacgccaa
     3121 cagaagtgcg tttctggggc gtgttttgtc aggagtttaa tctcgcgttt cgatggtgag
     3181 cgggcgaacc aggccgtaaa tcaccattgg tgatccgagg ccaatcagca ataaacggta
     3241 gataattgaa ggtttaatac gcttaatctg tactgtggat gttttactgg agttattcat
     3301 tagtttggcc aatagttcca ctatttcaat atgataaacc tcgtcctgtt gaaaaaccag
     3361 acagcacatt tcagaaagta tataaaaaac aatatgtcgt tatataaata aggaaattgc
     3421 actgcatcat ggcatttcat aatatgcgtt aattgttctt ttttatattc tttgagggaa
     3481 atatgttcct ggactatttt gcactgggag tgcttatttt tgtatttctg gtgatattct
     3541 atgggattat tattttacat gatattccct acctgattgc taaaaaacgt aatcatcctc
     3601 atgccgacgc catt
//
LOCUS       DAZSNG010000216         3588 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZSNG010000216 DAZSNG010000000
VERSION     DAZSNG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.220"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..77
                     /locus_tag="ACHW4Z_004109"
     CDS             <1..77
                     /locus_tag="ACHW4Z_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8883490.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHW4Z_004110"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHW4Z_004110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR8883491.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACHW4Z_004111"
     CDS             1490..2614
                     /locus_tag="ACHW4Z_004111"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR8883492.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHW4Z_004112"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHW4Z_004112"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR8883493.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          952 a          773 c          865 g          998 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtataa ttttcattat caaaatataa gatctaaaaa tactcttgat atagcatttg
     3541 gtattgacag aaattttctt tttggatgtg gtgtagccat cgcatcta
//
LOCUS       DAZSNG010000217         3548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZSNG010000217 DAZSNG010000000
VERSION     DAZSNG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.221"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHW4Z_004113"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHW4Z_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8883494.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHW4Z_004114"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHW4Z_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR8883495.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHW4Z_004115"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHW4Z_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR8883496.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZSNG010000218         3493 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZSNG010000218 DAZSNG010000000
VERSION     DAZSNG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.222"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..311
                     /locus_tag="ACHW4Z_004116"
     CDS             <1..311
                     /locus_tag="ACHW4Z_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR8883497.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHW4Z_004117"
     CDS             468..1217
                     /locus_tag="ACHW4Z_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR8883498.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHW4Z_004118"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHW4Z_004118"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR8883499.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHW4Z_004119"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHW4Z_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR8883500.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3493
                     /locus_tag="ACHW4Z_004120"
     CDS             3361..>3493
                     /locus_tag="ACHW4Z_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR8883501.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DAZSNG010000219         3414 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZSNG010000219 DAZSNG010000000
VERSION     DAZSNG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.223"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..238
                     /locus_tag="ACHW4Z_004121"
     CDS             <1..238
                     /locus_tag="ACHW4Z_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR8883502.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="ACHW4Z_004122"
     CDS             248..886
                     /locus_tag="ACHW4Z_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR8883503.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="ACHW4Z_004123"
     CDS             889..2052
                     /locus_tag="ACHW4Z_004123"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8883504.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="ACHW4Z_004124"
     CDS             2136..>3414
                     /locus_tag="ACHW4Z_004124"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8883505.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAZSNG010000220         3405 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZSNG010000220 DAZSNG010000000
VERSION     DAZSNG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.224"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(226..429)
                     /locus_tag="ACHW4Z_004125"
     CDS             complement(226..429)
                     /locus_tag="ACHW4Z_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883506.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHW4Z_004126"
     CDS             complement(431..586)
                     /locus_tag="ACHW4Z_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR8883507.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHW4Z_004127"
     CDS             complement(789..1196)
                     /locus_tag="ACHW4Z_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883508.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHW4Z_004128"
     CDS             1273..1500
                     /locus_tag="ACHW4Z_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR8883509.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHW4Z_004129"
     CDS             1484..1906
                     /locus_tag="ACHW4Z_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR8883510.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHW4Z_004130"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHW4Z_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3405
                     /locus_tag="ACHW4Z_004131"
                     /pseudo
     CDS             2768..3405
                     /locus_tag="ACHW4Z_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          878 a          753 c          916 g          858 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acatt
//
LOCUS       DAZSNG010000221         3378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZSNG010000221 DAZSNG010000000
VERSION     DAZSNG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.225"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            225..560
                     /locus_tag="ACHW4Z_004132"
     CDS             225..560
                     /locus_tag="ACHW4Z_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR8883511.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            718..1161
                     /gene="yjdN"
                     /locus_tag="ACHW4Z_004133"
     CDS             718..1161
                     /gene="yjdN"
                     /locus_tag="ACHW4Z_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR8883512.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1294..2082
                     /gene="phnC"
                     /locus_tag="ACHW4Z_004134"
     CDS             1294..2082
                     /gene="phnC"
                     /locus_tag="ACHW4Z_004134"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR8883513.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            2107..3123
                     /gene="phnD"
                     /locus_tag="ACHW4Z_004135"
     CDS             2107..3123
                     /gene="phnD"
                     /locus_tag="ACHW4Z_004135"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8883514.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3229..>3378
                     /locus_tag="ACHW4Z_004136"
     CDS             3229..>3378
                     /locus_tag="ACHW4Z_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR8883515.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT          867 a          936 c          859 g          716 t
ORIGIN      
        1 tgtgaataaa aaaaagaaca agtagagcat taacattatc ttaaataata aatagaggca
       61 aaaagattat tttctttttg cgtttccttt caaatgaaaa cgatcgtcgt ctaaaatcag
      121 cagtaccccc gacaaactca gggattttgt gtataattgc ggcctttttc ggcaatctgc
      181 cgttttttgg cgcttttgcc ctgctgactt ttgaggaaat ccacatgtca ttaccacact
      241 gcccaaaatg caactccgaa tacacttacg aagataacgg catgtacatc tgcccggaat
      301 gtgcctacga atggaacgac gcagaacctg cacaggaaag cgacgagctg atcgttaaag
      361 atgctaacgg caatctgctg gctgacggcg acagcgttac catcattaaa gatctgaagg
      421 tgaaaggtag ctcttcgatg ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg
      481 aaggcgacca taacatcgat tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg
      541 agtttgtgaa aaagaactga ttgtattgtg atcggtaagc cggataaggc gctcgcgccg
      601 catccggcaa cgatgccgac tgcctgatgc gacgcttgcg cgtcttatca ggcagcctct
      661 catctgtata aatttcgaac tacacttaac tggcttctct taactgaggt caccatcatg
      721 ccgttaagtc cctacctctc ttttgccggt aactgttccg acgcgattgc ctattatcaa
      781 cgtacgttgg gcgcggaact gctctataaa atcagcttcg gcgaaatgcc gaaatcagcg
      841 caggacagcg ccgagaactg cccttccgga atgcaatttc ccgataccgc catcgctcat
      901 gccaacgtgc gcattgccgg aagcgacatc atgatgagcg atgctatacc gtcaggaaaa
      961 gccagctact ccggctttac gctggtgctc gattcgcaac aggtcgaaga aggaaaacgc
     1021 tggtttgata accttgccgc taacggaaaa atcgaaatgg cctggcagga aactttctgg
     1081 gcgcatggct ttggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcgtc
     1141 aaacaacaac caacgcaata acccgccggg aggcccgccc tcccgcactg tcatcgaatt
     1201 cccgttaact cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc
     1261 acgatggagc cactttttta gggaggctgc atcatgcaaa cgattatccg tgtcgagaag
     1321 ctcgccaaaa ccttcaatca gcatcaagcg ctgcatgcgg ttgatctgaa cattcatcac
     1381 agtgaaatgg tggctctgct tgggccgtcg ggttccggca aatccaccct tttacgtcat
     1441 ttaagcggtt tgattaccgg cgataaatcc gccggtagcc atatcgagct actgggccgc
     1501 actgtccagc gcgaaggccg cctggcccgc gatatccgca aaagccgcgc caacaccggc
     1561 tacatctttc aacaattcaa cctggtgaac cgcctgagcg tactggagaa cgtgctgatt
     1621 ggcgcgctcg gcagcacgcc gttctggcgc acctgtttta gctggttcac cggcgagcag
     1681 aaacagcgcg cgttacaggc gctgacccgc gttggcatgg tgcattttgc ccatcagcgc
     1741 gtttccaccc tctccggcgg ccagcagcaa cgtgtggcga ttgcccgtgc gctgatgcag
     1801 caggcgaaag tgattctggc cgatgaaccc atcgcctcgc tggacccgga atcagcgcgc
     1861 atcgtgatgg acaccctgcg cgacatcaac cagaacgacg gcatcaccgt ggtcgtcacg
     1921 ctgcatcagg tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgtcagggg
     1981 cacgtcttct acgacggcag cagccaactg tttgataacg aacgttttga ccatctctac
     2041 cgcagcatta accgcatcga agagaacgcg aaagctgcct gatatcccca tcattgagga
     2101 aaacgaatga acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc
     2161 accctgttaa ccccggcaca cgccgaagag caggaaaagg cgctgaattt cggcattatt
     2221 tcaacggaat cacagcaaaa cctgaaaccg caatggacgc cgtttttaca ggatatggag
     2281 aagaaactgg gcgtgaaggt caacgccttc tttgccccgg actacgcggg cattatccag
     2341 gggatgcgct tcaataaagt ggatattgcc tggtacggca acctgtcggc gatggaagcg
     2401 gtggatcgcg ccaatggcca ggtcttcgcc cagacggtcg cggcggatgg atcgccgggt
     2461 tactggagcg tgttgatcgt caacaaagac agcccgatca acaacctgaa cgatctgctg
     2521 gcgaagcgga aagatctcac cttcggcaat ggcgatccta actccacctc tggcttcctc
     2581 gtccccggtt actacgtctt cgctaaaaac aatatctccg ccagcgactt caagcgcacc
     2641 gtcaacgccg ggcatgaaac caacgcgctg gccgtcgcca acaagcaagt ggatgttgcc
     2701 accaacaaca ccgaaaacct cgacaagctg aaaacctccg cgccggagaa gcttaaagag
     2761 ctgaaggtga tctggaaatc gccgctgatc ctaggcgatc cgatcgtctg gcgcaagaat
     2821 ctgtccgaaa ctaccaaaga caagatctac gacttcttta tgaactacgg aaaaacgccg
     2881 gaagagaaag cggtgctgga acgcctgggc tgggcgccgt tccgcgcttc cagcgacctg
     2941 caactggtgc cgattcgcca gctcgcactg tttaaagaga tacaaagcgt gaaaggcaat
     3001 aaagggctga atgagcagga caagctggcg aaaaccaccg agattcaggc gcagctggat
     3061 gacctggacc gcctgaacaa cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag
     3121 taaatcgtag gtcggataag acgccccggc gtcgcatccg acaatgtgta ggcgttgatg
     3181 ccggatgcgg tgcaagcacc ttatccggcc tacagaccgg agccaaacat gcaaaccatc
     3241 accatcgccc cacccaagcg tagctggttc tcgcttctga gctgggccgt tgttctcgac
     3301 gtgctggtcg tctcgtggca gggcgcggaa atggccccgc ttacgctgat taaagacggc
     3361 ggcaacatgg caaccttc
//
LOCUS       DAZSNG010000222         3357 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZSNG010000222 DAZSNG010000000
VERSION     DAZSNG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.226"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            19..897
                     /locus_tag="ACHW4Z_004137"
     CDS             19..897
                     /locus_tag="ACHW4Z_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8883516.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHW4Z_004138"
     CDS             894..2285
                     /locus_tag="ACHW4Z_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8883517.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHW4Z_004139"
     CDS             2282..2539
                     /locus_tag="ACHW4Z_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883518.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHW4Z_004140"
     CDS             2634..2834
                     /locus_tag="ACHW4Z_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR8883519.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHW4Z_004141"
     CDS             2827..3207
                     /locus_tag="ACHW4Z_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR8883520.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          886 a          728 c          973 g          770 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtg
//
LOCUS       DAZSNG010000223         3336 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZSNG010000223 DAZSNG010000000
VERSION     DAZSNG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.227"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            274..549
                     /locus_tag="ACHW4Z_004142"
     CDS             274..549
                     /locus_tag="ACHW4Z_004142"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR8883521.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            747..2111
                     /locus_tag="ACHW4Z_004143"
     CDS             747..2111
                     /locus_tag="ACHW4Z_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8883522.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2161..2370
                     /locus_tag="ACHW4Z_004144"
                     /pseudo
     CDS             2161..2370
                     /locus_tag="ACHW4Z_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2464..2655)
                     /locus_tag="ACHW4Z_004145"
                     /pseudo
     CDS             complement(2464..2655)
                     /locus_tag="ACHW4Z_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2696..3193)
                     /locus_tag="ACHW4Z_004146"
                     /pseudo
     CDS             complement(2696..3193)
                     /locus_tag="ACHW4Z_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          819 a          738 c          790 g          989 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta attttctcag gaaaaacata aaattactga ctaaatattt attttttgaa
     2161 tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta tggatacagt
     2221 atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt tcagcttcat
     2281 ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat cctgtatgca
     2341 ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg cgatatatct
     2401 ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg tgatgtcaga
     2461 tgtccccagt aagactatgt tttcattgcg ctctatgaag ctgagtgagc gtaacgactg
     2521 gagctgcttc tgcggtgctc cggtggcgaa cgtgaagtca tactcttcga acattttcac
     2581 cgccgggaag gctgccattc gggtatacat cgcctgttta cgttgatgac gtgccagttt
     2641 ttcttcatga agcatgatgc tccaagtaaa cgttcaccga acaccgtatt gacgattatc
     2701 ctgatggtag caccaccttc catggcagga ggtcgtcaac tcggttggaa ggccattccg
     2761 gcagtacgct cagaatatgg cgcagatacg cttccggatc gataccgttc agacggcagg
     2821 tgccgatcag cccgtacagc agtgcaccac gctcgccgcc gtgatcgcta ccgaagaaca
     2881 cgtaattttt ctttccgaga cagactgcac gaagcgctct ttccgctgtg ttattgtccg
     2941 cctccgccag accgtcatca ctgtaataac agagggcgtc ccactgattc agtacatagc
     3001 tgaatgcttc gcccagtctg gattttttcg acagcgtgcc attcttctcc accatccatt
     3061 catgcagcga cgtcagtaac gctttgcttc gctgctgcct gactgcaaga cactctgact
     3121 ctggtaatcc ccgtatttca tcctcgatgg cgtacagttc actgattcgc ttcagggctt
     3181 cttctgccgt cgcacgcaag taatccgggg cccgctatgc cgcgctctat cgggcgggac
     3241 ggggctggcg ctttgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg
     3301 ctgccagatc atcgtttccg atggaagcat aataag
//
LOCUS       DAZSNG010000224         3325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZSNG010000224 DAZSNG010000000
VERSION     DAZSNG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.228"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..208
                     /locus_tag="ACHW4Z_004147"
     CDS             <1..208
                     /locus_tag="ACHW4Z_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883523.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHW4Z_004148"
     CDS             269..457
                     /locus_tag="ACHW4Z_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883524.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHW4Z_004149"
     CDS             454..1281
                     /locus_tag="ACHW4Z_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8883525.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHW4Z_004150"
     CDS             1325..2074
                     /locus_tag="ACHW4Z_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8883526.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHW4Z_004151"
     CDS             2071..2640
                     /locus_tag="ACHW4Z_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883527.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHW4Z_004152"
     CDS             2637..2759
                     /locus_tag="ACHW4Z_004152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883528.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHW4Z_004153"
     CDS             2764..3006
                     /locus_tag="ACHW4Z_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8883529.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHW4Z_004154"
     CDS             3010..3156
                     /locus_tag="ACHW4Z_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883530.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHW4Z_004155"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHW4Z_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR8883531.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZSNG010000225         3294 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZSNG010000225 DAZSNG010000000
VERSION     DAZSNG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.229"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(22..429)
                     /gene="mxiM"
                     /locus_tag="ACHW4Z_004156"
     CDS             complement(22..429)
                     /gene="mxiM"
                     /locus_tag="ACHW4Z_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR8883532.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(422..1117)
                     /gene="mxiN"
                     /locus_tag="ACHW4Z_004157"
     CDS             complement(422..1117)
                     /gene="mxiN"
                     /locus_tag="ACHW4Z_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR8883533.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1059..1586)
                     /locus_tag="ACHW4Z_004158"
     CDS             complement(1059..1586)
                     /locus_tag="ACHW4Z_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFR8883534.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(1583..2308)
                     /gene="mxiJ"
                     /locus_tag="ACHW4Z_004159"
     CDS             complement(1583..2308)
                     /gene="mxiJ"
                     /locus_tag="ACHW4Z_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR8883535.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2314..2607)
                     /gene="mxiI"
                     /locus_tag="ACHW4Z_004160"
     CDS             complement(2314..2607)
                     /gene="mxiI"
                     /locus_tag="ACHW4Z_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR8883536.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(2620..2871)
                     /gene="mxiH"
                     /locus_tag="ACHW4Z_004161"
     CDS             complement(2620..2871)
                     /gene="mxiH"
                     /locus_tag="ACHW4Z_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR8883537.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(2879..>3294)
                     /gene="mxiG"
                     /locus_tag="ACHW4Z_004162"
     CDS             complement(2879..>3294)
                     /gene="mxiG"
                     /locus_tag="ACHW4Z_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR8883538.1"
                     /translation="LVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHNAR
                     GDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIEFS
                     VLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
BASE COUNT         1036 a          640 c          424 g         1194 t
ORIGIN      
        1 ataaatattt tcaacttatt actaccatgt cgaatcatct gcctctctcc ctgcttcaaa
       61 gacactaatt aatgaatcga aggttgtagt cttaatatag aaatcaatat tgttatatat
      121 ggacaatgat gcataaaatc cctcatgcaa atcgacatat tgatttgcat ctatgagttt
      181 attaagaagt gaactttttt gtgcagctgt aagataagga aaacgtgaat tgaataattg
      241 ttcaatctta ttgtgcagtt catcattatc tatggaattt aattcactct gtgagaaata
      301 ttgcattatt tcatagatta tatcctcatc aaatgattgc gaagatgata atctctcatt
      361 taaattaaca acttcttcac tatttaatct ttgctgcaga gcattgcttg catttatttg
      421 attaatcatt aacaggattc tcttttaaca agtcttctgc aatttgtctc agtttcttat
      481 gtgctaactc tttgaaatat tttttatcat tttttgttaa tttttccgca agttctattt
      541 taacacctga tattacatcc tgaggactaa actccactac ttgattacca ctacagaata
      601 tataattatt cccttcagaa tattttaaaa taatcttgac atctgagcga tatttatgga
      661 gatcaagttc taactttttc ctcagtgcta aattacactt gggcaaaacc acttttaatt
      721 ctgttactgt attacgcaac gaactaagcc actgctccaa taaagtgcta atgatcctct
      781 catcatgtaa attttctgaa agcaaactag ttatatagtc ctcaatattt cttctatttt
      841 cattttgctt taacttccat tcacaaatat agtcaataat atgctcaaaa gcagtctgta
      901 taccaatttg ataaccatca catacagcat ctatacgcag tgattcagct tttttcgtag
      961 cctcctttat aacacaaatt gctttcttct tactctcagt gtctctatct tttatggttt
     1021 tacataattc cttttcaatt ctctttataa caacaccatc ataggcatga tgtctggata
     1081 ccggtaaagt gcctttttgc atattgcata ccttcattta atcgggacaa atttctttct
     1141 acaggcgtat aatttccctc caaagcaatt ggattaaata ataagtttat tctttcatat
     1201 ataccaacag atatattttc cttccacgca ataagatgcc aaaaaccact tcgaatagcg
     1261 gcctcccctt gcttactgat tttgccccca taataactaa taaccatctc actatgatta
     1321 attaaccgag actcttgact accgataaaa taagcaacag ctggaagtaa attccagttg
     1381 gcaacaaaca tagcggttac cccgcttaaa ggttcaatat cgcaatttag atcatattta
     1441 ttaattatga gattattaat ctcacttctg attacgccat tttctaacag ttcagaaggc
     1501 aaattcaacc gatttctatt tatatagaac gctggatcaa aaattattga aagatatttt
     1561 ttataaattc catccattct tatcatattt tgtttctctt gaaccaccct gttttgaatg
     1621 cccataccaa aagaatgaca actagtacag ccatcccgag aaataaatat attacttcat
     1681 ttgttaaaaa ttccgattta acttccttaa caggttgtac atttgtataa acatattctt
     1741 ctttcggagt taatatgaca gatatatttt catacttaac atcagaaaag gtgtttttca
     1801 aaaatcgctt aatattacta actaatagtt cagactcttt cggtgagtca tatatagcga
     1861 ttactgatat atgcatcggt tttgaagata tatttttttc ttcaagatca tagctaacat
     1921 gtatttttgc cgaaataaca ccaccaatag aaactaaaga ctgttccagc cgttgctcaa
     1981 tagcactata taaacgggcc ttttcagctc ttggagaaga cactaatgaa tctgtaggaa
     2041 acatttgtga gatatcaact ctctccggat ttggcaaatc gtacatgcgc atcaaatcaa
     2101 ctgccgatgc aaatgtcccc ttttcgactt gtaccgagat cccctgttta cctccatcaa
     2161 cttttctagc agtaatatta tggcgttcta gcacagatat tatttcattt gcctgtcttt
     2221 gagataaatt agaaattaac tcttcacgtt gctcacatcc aatcaacatc agcaacaaga
     2281 ataaaataaa acctttatac ctaatcatat aaattaagac tttaataaag tttcaacagc
     2341 cgaaactgtt tttcttgcta acgtgccagc taatgatact cctattgaat aatttgagag
     2401 cgtcgtctgt aacttggcca aagattctgg gtttaatgat tctggattgc ttaccatctc
     2461 cattatttgt gatacttgaa tatctgtatc catcttaata tcactatatt tagccgacac
     2521 gacgtcttcc agacttgata tctcttgaga ttgaaaatca ctggctttga taatatcaac
     2581 ctgattgact ggataaatgt aattcatgaa tgctccctat tatctgaagt tttgaataat
     2641 tgcagcatca acatccttaa tcactttcac tgtattggat tgcgcgttcc tatataatgt
     2701 atattcagat aatttacttt ggtattcagc cagcaactgt ggattcgaag gatttttagc
     2761 taatttatct agtgccaatg ttagttcacc ttgtaatgtt tgagttccat catcaaaagt
     2821 ttcagataat gaactcaatg tccaatcatc attcggtact gtaacactca ttttatcctc
     2881 acttattttt atctaaaaaa aaccagtgtt tatcattcaa cattacataa ctgtctttgc
     2941 tgttaagata tgatttacct ttaaaatcat catctatcaa aagtacagaa aactcaatat
     3001 actgatctcc atatatattt ttatgttcag aaatcagtct attaatgagc tcaagctttt
     3061 catctgtcag ttcttctctg acagaatatg taactttatt attttcacaa atctctttat
     3121 actggacatt tacctttgtg aaaatacctt tagcatcccc ccttgcatta tgatcgctaa
     3181 gatagttgaa tttaatattt ttgatatacg gaaattcaac aatcagagca tttttcaatt
     3241 tatcaagttc ggtatctttt gaactatttc tttctttact taaaaacacc agta
//
LOCUS       DAZSNG010000226         3193 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZSNG010000226 DAZSNG010000000
VERSION     DAZSNG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.230"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="ACHW4Z_004163"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="ACHW4Z_004163"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR8883539.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="ACHW4Z_004164"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="ACHW4Z_004164"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR8883540.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="ACHW4Z_004165"
     tRNA            2656..2731
                     /locus_tag="ACHW4Z_004165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>3193)
                     /locus_tag="ACHW4Z_004166"
     CDS             complement(2746..>3193)
                     /locus_tag="ACHW4Z_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8883541.1"
                     /translation="ESKSNRAIGVALNETACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          754 a          807 c          878 g          754 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc atttaaatca ataagttaca catcattagt
     2761 accttcctta tttcttgact gggacaaatt tgggaccgat gggttcagaa tcaagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcgaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgataac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcccgtct
     3001 cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc ttccttactg ttggcgcttt
     3061 cgttccgtct ggtttggtac agctttcctt gtacacaaat acccaacggt gatgatttcc
     3121 gatttgtttt ttcaatacgc gacatgcagt ttcattcagc gcaacgccaa ttgcgcggtt
     3181 tgatttactc tct
//
LOCUS       DAZSNG010000227         3181 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZSNG010000227 DAZSNG010000000
VERSION     DAZSNG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.231"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(19..645)
                     /gene="dsbA"
                     /locus_tag="ACHW4Z_004167"
     CDS             complement(19..645)
                     /gene="dsbA"
                     /locus_tag="ACHW4Z_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR8883542.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(662..1648)
                     /gene="srkA"
                     /locus_tag="ACHW4Z_004168"
     CDS             complement(662..1648)
                     /gene="srkA"
                     /locus_tag="ACHW4Z_004168"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR8883543.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1725..1994)
                     /locus_tag="ACHW4Z_004169"
     CDS             complement(1725..1994)
                     /locus_tag="ACHW4Z_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR8883544.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2064..2648
                     /gene="mobA"
                     /locus_tag="ACHW4Z_004170"
     CDS             2064..2648
                     /gene="mobA"
                     /locus_tag="ACHW4Z_004170"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR8883545.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2630..3157
                     /gene="mobB"
                     /locus_tag="ACHW4Z_004171"
     CDS             2630..3157
                     /gene="mobB"
                     /locus_tag="ACHW4Z_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR8883546.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          836 a          771 c          763 g          811 t
ORIGIN      
        1 tatgtaattt acattgaatt atttttcctc ggacagatat ttcaccgtat cagcatactg
       61 ctgaacaaaa acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc
      121 gttaacaaac atcgccggaa caccacgcaa ttgcacatca gctgcggctt tttcctgctg
      181 agcgaccaga gatttcacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat
      241 acctgcattg ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg
      301 tacgccttca aacagcggaa cagtcacttt gtcttccacg cccagcgcca tcgccacagc
      361 ccatgcctga gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt
      421 catcttcacg ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc
      481 aaactgatag cagtgcgggc agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc
      541 taccggtttt tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct
      601 aaacgctaaa actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg
      661 attaatacat aggtgttaat tgcaaagggg gttcttgtag aacttttgcc tgttctataa
      721 aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga
      781 acgcgggatc agcccaatgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc
      841 gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc gctaaattct tcataagctt
      901 caataatagt ttccagttgc atccgctgct gggctttatc gccattgagc aacatccaca
      961 aatcctgaat agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc
     1021 gccagagaat attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc
     1081 gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc tttcagaaat gccgctttca
     1141 acccggaagg tatcagtgta gcgtcctcaa acagcttgcg aggctcaatg agatattcat
     1201 tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg ccccgtctgg tgcatacgcc
     1261 ctaaataacg cccaaccgct tccatctgat cgatattatc agcttcgaac tggcgaccac
     1321 cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa
     1381 aggccacagg tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt
     1441 cttcgaggat ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc
     1501 gacgctcttc gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac
     1561 cggaatccac ccggatccct tgcttaaaca gagcgtccat gatggtatcc gggtgtagtg
     1621 tctggaaagt aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa
     1681 ggatatcatt tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg
     1741 ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg
     1801 atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc
     1861 aaatccgcca gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac
     1921 aagttaaagt ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga
     1981 cgtttacatt tcatagtaat gctccttact cttgagacag acacgttagc agggtcaatc
     2041 ccacaataaa agaggcgata tcggtgaatc taatgacgac gataacaggc gttgtgctgg
     2101 caggcggtaa agccagacga atgggcggcg tagataaagg attgctggac ttaaatggca
     2161 aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca gctctctcac gtcgtggtta
     2221 atgctaatcg tcatcaggaa atctatcagg caggcggtct gaaagtgatt gaagattcac
     2281 tggcggatta cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg
     2341 agtggttttt gttttgtccg tgcgatacgc cttacattcc ccatgattta gccgcccggc
     2401 ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc
     2461 cgactattgc tctggtaaac cgcgctattg agcctttatt actggaatat ctgcaagcag
     2521 gagaacgccg ggtaatggca tttatgcgtc tggctggcgg tcatgcggtt gatttcagcg
     2581 atcataaaga tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa
     2641 aacgatgata ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt
     2701 gaaaaaactg atcccggcat tatgcgccag agggatccgt ccggggctga ttaagcatac
     2761 gcaccatgat atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg
     2821 cgcggtacaa accatcgttg ccagccagca acgatgggcc ttgatgacgg aaacaccaga
     2881 cgaagaagag ctggatctac attttctcgc aagtcgaatg gatacctcaa agctggattt
     2941 gattctggtc gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg
     3001 agccggacat cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga
     3061 tgtgccgctt aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga
     3121 ttttgtggtg gagtggatgc aaaagcagaa cggataagca agagtaggct ggatcagacg
     3181 c
//
LOCUS       DAZSNG010000228         3179 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZSNG010000228 DAZSNG010000000
VERSION     DAZSNG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.232"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW4Z_004172"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW4Z_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR8883547.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHW4Z_004173"
     CDS             complement(1772..1906)
                     /locus_tag="ACHW4Z_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8883548.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHW4Z_004174"
     CDS             complement(2552..2722)
                     /locus_tag="ACHW4Z_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8883549.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZSNG010000229         3166 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZSNG010000229 DAZSNG010000000
VERSION     DAZSNG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.233"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..885
                     /locus_tag="ACHW4Z_004175"
     CDS             <1..885
                     /locus_tag="ACHW4Z_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8883550.1"
                     /translation="QLRDITESKIYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYT
                     PKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLI
                     DECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALND
                     TACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDF
                     RFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILN
                     PSVPNSSQSKNKEGTNDV"
     gene            1234..2406
                     /locus_tag="ACHW4Z_004176"
     CDS             1234..2406
                     /locus_tag="ACHW4Z_004176"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8883551.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2460..>3166)
                     /locus_tag="ACHW4Z_004177"
     CDS             complement(2460..>3166)
                     /locus_tag="ACHW4Z_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883552.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAEQPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
BASE COUNT          996 a          660 c          630 g          880 t
ORIGIN      
        1 caactaagag acattactga atcaaaaatt tattcagcaa tgcagaaaat gacgaaccgg
       61 cgtcatgagg aaaactggag actcagggca gaagcatgca gaaaaaaagg gaaacctgtt
      121 ccagaataca cgccaaaacc agcgtccgtt gcaacgaagg ctacgcatct ttcatttata
      181 aaggccctac taagagccgc agagcgtgaa tggaaaatgc tggataaggc accaattatt
      241 aaagtgcctc aaccaaagaa taaacggatc cgctggctgg agccccatga agcacaaagg
      301 ctgattgatg aatgtccgga gccattaaag tctgttgttg aatttgcact ggcaacaggc
      361 ttaagacgct cgaacatcat caaccttgaa tggcaacaaa tagatatgca gcgccgggtg
      421 gcatggataa acccggaaga gagtaaatca aaccgcgcaa ttggcgttgc gctgaatgat
      481 actgcatgtc gcgtattgaa aaaacaaatc gggaatcatc accgttgggt atttgtgtac
      541 aaggaaagct gtaccaaacc agacggaacg aaagcgccaa cggtcaggaa gatgcggtat
      601 gacgcaaaca cagcctggaa agcggcgctg agacgagcag gtattgatga tttcagattt
      661 cacgacttga gacacacctg ggcaagttgg ctggttcaag ccggagtccc gttgtcagtg
      721 ttacaggaaa tgggaggctg ggagtctatc gaaatggttc gtcgatatgc tcaccttgca
      781 cctaatcacc ttaccgaaca cgcacggcaa atagactcga tcctgaaccc atcggtccca
      841 aattcgtccc agtcaaaaaa taaggaaggt actaatgatg tgtaacttat tgatttaaat
      901 ggtgccgata ataggagtcg aacctacgac cttcgcatta tgaatctgta gcaccaatca
      961 taactatctg ttttagcaag cattaaccgc attcactaag ctatagttga tgacacaaac
     1021 agaaagttga tgcatgatgt tgccatatat atgtcacaaa tacggcacaa cgatcttcaa
     1081 acatatagcc acacattcac agaagagcac aaagccttgc aatccagtgc aaagcttggt
     1141 gtgtctccgt tttgtttcat cagttacaca taactatatg atcattaact aaaagtcagt
     1201 atacttggag aaattttaat tgcgtaatag agcatgcata tgattgaaat taacagttta
     1261 ttattaataa catccgtgat attgatgtcg ttattagctg tggggttatt tgataaaatt
     1321 tccccaataa atcttgttga gcacggcagg aataatcaaa tagatggaat gcgggggttc
     1381 ttagcaattt tcgtgcttat tcatcacgca gcaatttgga atggatactt gtcatctgga
     1441 gtatgggaag caccttcatc aaatctgtta gcaaatttag gccaagttgg tgtgtcattc
     1501 ttttttatga ttactggtta tctgttcttt tcaaagatta tctctggaga tcaggactgg
     1561 acaaggcttt atgtatcaag attactacga ttaaccccaa tgttcatagt tagcctatgt
     1621 ctcattttta tcattgtagg ttttaagtct ggatggagaa tgcaggtatc cacagaagag
     1681 ctttttgtgt caataatgaa gtggttgcca ttcactgcgc taggtatgcc gaacattaat
     1741 gacgtaaaag attcatttac tatcaatgcc gctgtaacat ggacacttgt atatgaatgg
     1801 ttcttttatt tttctcttcc ggtaatttcc gcgctcataa aaagaaaagt cagtatttac
     1861 atggttatga ttagcgcaat atcgctattt gttttcattt tatttttcag taaaatacac
     1921 atagcatcat ttctttttgg gctcctggca tttttgctaa ataaatcaaa gatagtcaat
     1981 ggaattgcca aagcaaaagt aacaccaatt ataataactg cgataatggt ttttgaaatg
     2041 acgtatttca aaacaactta cgcaccgcta ccactgattc tttgtggaat aacatttatc
     2101 attatcgcat caggttgtga tttgtatgga atattaagat taaatataac cagaaagcta
     2161 ggagaaacta cctacagcgt ttaccttctg catgggatat tcctttattg cctaatgacg
     2221 tggattattc ctaataatta cactgaaaat acatttatca tactggtaag cacaactgca
     2281 tttcttatta cgttcacatc atgcctaact tttaaattaa tagagacgcc attcatcaaa
     2341 ttaacaaaac aaaccacaac tttagtaaaa gaattaatac ccacattaac aaacaacaat
     2401 caataaacaa taaacataag ccgccctaca tatatgtcca aatggggcgg cgaatgtatt
     2461 tatattagtc tggtaatgct aaatgcaaac gaactggaga ttcctgcccc gctaagcgta
     2521 ttgcgcaggg tcaccctaaa tgtatctacg ccaatataag taacatcaca actaactgaa
     2581 tcaggcgcag atataggtgt tatctgcaca atataaccgc tagcaactgc tgcgagcgga
     2641 tgagaaaagc taacatcata cacacctgta gaaaccacgt taaccgatga aacaccagta
     2701 gcaacgtcga tactaccatt ggcgttaatt tttcccgagc gtgtgactga caatgcagaa
     2761 gccgatgtta ccttactggt tgtttctgta aaaacacacc caatgtcaac tacctcgcaa
     2821 ttagctcctg ttatccagta tggttgttct gcacttggct gataatcccc tgcgctctgg
     2881 aacgttgtgc cgttgagtat gactgtaact tttcccccac ctgagcttgt tacttgaata
     2941 ttcgtgtgcg tgtatcttgc actgctcacg cgatgaaaat ttccaccgtt tataaccaca
     3001 gttacagggc gcgtcccagt attatctata gcaaggtctg cccccccggc attagcctca
     3061 aagtatgggt tgtttaatac caaagcgcac gaaccgttaa ggccatttat agctaattgc
     3121 gcaccacctg tactggaatc cccctgagta ccatttcctt cacaag
//
LOCUS       DAZSNG010000230         3164 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZSNG010000230 DAZSNG010000000
VERSION     DAZSNG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.234"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            87..1187
                     /gene="ompC"
                     /locus_tag="ACHW4Z_004178"
     CDS             87..1187
                     /gene="ompC"
                     /locus_tag="ACHW4Z_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8883553.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1351..1836
                     /locus_tag="ACHW4Z_004179"
     CDS             1351..1836
                     /locus_tag="ACHW4Z_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8883554.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            1946..3164
                     /locus_tag="ACHW4Z_004180"
                     /pseudo
     CDS             1946..3164
                     /locus_tag="ACHW4Z_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT         1063 a          628 c          548 g          925 t
ORIGIN      
        1 ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa ttcgtttaga aaatacgttt
       61 aacgataatt aaaaggattc tgttaaatga aattaaaaat agttgcggtg gttgtaactg
      121 gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac
      181 tcgaccttta tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg
      241 gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga
      301 ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc
      361 gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg
      421 actatggtcg taactacggt gtcggctacg acattggttc atggaccgat atgttgccag
      481 aatttggtgg cgatacctgg agtcagaaag atgtcttcat gacataccgt accaccggtc
      541 tagcaaccta tcgcaactac gatttctttg gcttaattga agggctgaac tttgccgcgc
      601 aatatcaagg caaaaataaa cgtactgaca acagtcatct ttatggtgct gacgacacgc
      661 gtgccaacgg tgacggtttc ggtatctcct caacttatgt ttatgatggc tttggtatcg
      721 gtgcggtata taccaaatcc gatcggacaa atgcgcagga aagagccgca gctaatcctc
      781 tcaatgcctc cggtaagaat gcagaactat gggccacagg tataaagtat gatgccaaca
      841 acatctactt tgcagctaat tacgatgaaa cattaaacat gaccacctat ggggatggtt
      901 atatctctaa caaagcacaa agttttgaag tggtggcgca atatcaattc gacttcggct
      961 tgcgcccatc actcgcttac ctgaaatcga aaggcagaga tctgggccgc tacgccgacc
     1021 aggacatgat tgagtatatc gacgttggtg cgacgtattt cttcaacaaa aatatgtcga
     1081 cctatgttga ttataaaatc aacctgattg atgaaagcga ctttacccgt gccgtagata
     1141 ttcgcaccga taacatcgtc gctacgggca ttacctatca gttctaacgt cctctcctga
     1201 ccagcccttc tctgggctgg tttttgattt aagtgctgct gtatgactcc ttaagtaaaa
     1261 atgagcaatt cttgatattt ctggtcattc acctcaatta atattattcc atatcctccg
     1321 gggacatctt actataatat ttattgatga gtgattatga ccaatattaa tacagcttgt
     1381 gtaaaaaata atgccagtta tcaacttaat aacgcattac ctaacaaaga aaccatctcc
     1441 agcaattttt gtgaacgact agaacaatgg ggtaataaat cggttaataa tggtgaagaa
     1501 agagcaattg ccgtggagcg aattaaagaa gcttacaatt cgaacatggc atctcttgat
     1561 ttgtcatatc ttgatttgag tgaattaccg cctatcccct cgacagttaa tacgttaaat
     1621 ctggaaaaca attgtctaac ttgtcttgac ttagctgaca atgccagtct cgttaatatc
     1681 aacctcagcg ttaataaaat taacacgata acctttccaa aagaaccaaa actggaaaac
     1741 atttatatta atcataacaa tctggaaagt ttagatttaa aaaatcagca ttcattggtt
     1801 aatctggaag cgcaaaataa tagcctaaaa aaatgatatt tctgatagtt ataaactgaa
     1861 atttcttaat cttgattata acaagctagc atcgctggat gtctcccggc aagaatctct
     1921 gattgagtta agtgcccatc acaatatgat cactgacctt atattacaca atcatcccag
     1981 gatgaaaaca atcactttaa acgacaacca tattgcacat ttaaacgcca aaaacactac
     2041 aaaactggaa tatttaaact taagcaataa caatttactg ccaaccaatg acattgatca
     2101 actaatatca tcaaaacatc tttggcatgt attagttaac ggcatcaaca atgatccact
     2161 tgcacaaatg cagtactgga ctgcagtaag aaatataatt gatgacacta atgaagtgac
     2221 cattgattta tcaggactta atttaaccac tcaaccacca gggctgcaaa acttcacctc
     2281 tatcaatctt gataataacc aactcacaca ttttgatgca accaactacg atagactcgt
     2341 aaagctaagt ctgaatagta atgctcttga gtcaataaat tttcctcaag gcagaaatgt
     2401 aagtattaca catatatcta tgaataataa tgctctcaga aatattgata tagataggct
     2461 ttcatcagtt acttatttta gtgcggcaca taatcaacta gagtttgtgc aattagaatc
     2521 ttgcgaatgg ctgcaatacc tgaatctcag ccataatcaa ttaactgata ttgttgcagg
     2581 taataaaaat gaactcttac tgctggatct atcccataat aaactaacaa gtttacacaa
     2641 tgtcttattt cccaacttga atacgttact tattaacaac aatttgcttt ctgaaattaa
     2701 aatattctat agcaacttcc gcaatgttca gacattaaac gctgctaaca accagttgaa
     2761 atatataaat cttgatttcc tgacttatct tccatctatc aaaagtttaa gactggacaa
     2821 taataaaata acccacattg atactaataa tacatccgat attggaactt tattccccat
     2881 aataaaacag agcaaaaact taaatttttt aaatgtttct gggaagaaca attgacctac
     2941 tatgcagctc atgttattta atttgttttc cccagcactt aagcttaata ctggcctggc
     3001 aattctttcg cctggtgcat ttgaagttca ctctgacgga atagatgcgg ataacgaatt
     3061 gtttcactat actattaaaa aagcatatac cccatataat atacacactt acaagacaga
     3121 ggaagttgta aaccagagga atataaaagt taaaaacatg acct
//
LOCUS       DAZSNG010000231         3069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZSNG010000231 DAZSNG010000000
VERSION     DAZSNG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.235"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..198
                     /locus_tag="ACHW4Z_004181"
     CDS             <1..198
                     /locus_tag="ACHW4Z_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8883555.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="ACHW4Z_004182"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="ACHW4Z_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR8883556.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="ACHW4Z_004183"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="ACHW4Z_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR8883557.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="ACHW4Z_004184"
     CDS             1822..2577
                     /locus_tag="ACHW4Z_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR8883558.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          799 a          683 c          728 g          859 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggca ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggagttatgt tcaacaattt
     2701 aattttagat atacatataa cttgttctat taataaatca cctcgtattc tgactcggcc
     2761 cctggcgcag caaagtcagg catttatact ctttttaaca catagaaaat tcatatcaat
     2821 ataataaaaa cataaaatat ttgcatatac atataatctt aaagtttagt ctatttaatg
     2881 ttcaatgaaa tatttctacc tgtataatct ttaaagatgt tgaacatata ttcacattaa
     2941 atatgattat gtacttgtta caaggataag gttataaatg aataaagtta caaaaacagc
     3001 tattgtgggc ttacttgcgc ttttcgcagg taatgccgct gcaaccgatg gtgaaatagt
     3061 ttttgatgg
//
LOCUS       DAZSNG010000232         3069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZSNG010000232 DAZSNG010000000
VERSION     DAZSNG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.236"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..498
                     /locus_tag="ACHW4Z_004185"
     CDS             <1..498
                     /locus_tag="ACHW4Z_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8883559.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHW4Z_004186"
     CDS             578..1273
                     /locus_tag="ACHW4Z_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8883560.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHW4Z_004187"
     CDS             1302..>3069
                     /locus_tag="ACHW4Z_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8883561.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZSNG010000233         3067 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZSNG010000233 DAZSNG010000000
VERSION     DAZSNG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.237"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHW4Z_004188"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHW4Z_004188"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR8883562.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHW4Z_004189"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHW4Z_004189"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR8883563.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHW4Z_004190"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHW4Z_004190"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR8883564.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHW4Z_004191"
     CDS             complement(1895..2809)
                     /locus_tag="ACHW4Z_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR8883565.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEEPEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          798 a          714 c          814 g          741 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccggtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattaccta gaaccacggc agcctgacgg ctgcaaattg aaaactgccc ctgttcgtcc
     2941 tggtgttgcc gcacgctccg cagaatatgt atgctctgag gacctgtttt attaaggact
     3001 catcctctta tgttgacccg caaaagtata gataccgttc tgctctctgt tggtgctgag
     3061 aagctct
//
LOCUS       DAZSNG010000234         3002 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZSNG010000234 DAZSNG010000000
VERSION     DAZSNG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.238"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHW4Z_004192"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHW4Z_004192"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR8883566.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>3002
                     /locus_tag="ACHW4Z_004193"
     CDS             1649..>3002
                     /locus_tag="ACHW4Z_004193"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8883567.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     H"
BASE COUNT          760 a          694 c          793 g          755 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
     2161 ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
     2221 gaaacgcatg attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt
     2281 gacctacacc ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca
     2341 ggccgacacc ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctagc
     2401 accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag
     2461 tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga
     2521 cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg
     2581 tacttttgcc atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt
     2641 cctgcgcctc ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc
     2701 ttatctggcg gatgaaatcg ccaaacaggg gccgtatgag ttcatctgta cgggtcgccc
     2761 ggacgaaggc atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac
     2821 cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct
     2881 cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat
     2941 ggactttgct gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca
     3001 cc
//
LOCUS       DAZSNG010000235         2951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZSNG010000235 DAZSNG010000000
VERSION     DAZSNG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.239"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHW4Z_004194"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHW4Z_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR8883568.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHW4Z_004195"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHW4Z_004195"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR8883569.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHW4Z_004196"
     CDS             2603..>2951
                     /locus_tag="ACHW4Z_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8883570.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZSNG010000236         2932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZSNG010000236 DAZSNG010000000
VERSION     DAZSNG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.240"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHW4Z_004197"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHW4Z_004197"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR8883571.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHW4Z_004198"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHW4Z_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR8883572.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHW4Z_004199"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHW4Z_004199"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR8883573.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHW4Z_004200"
     CDS             2756..>2932
                     /locus_tag="ACHW4Z_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883574.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZSNG010000237         2894 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZSNG010000237 DAZSNG010000000
VERSION     DAZSNG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.241"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..308)
                     /locus_tag="ACHW4Z_004201"
     CDS             complement(<1..308)
                     /locus_tag="ACHW4Z_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8883575.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHW4Z_004202"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHW4Z_004202"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR8883576.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHW4Z_004203"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHW4Z_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR8883577.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZSNG010000238         2878 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZSNG010000238 DAZSNG010000000
VERSION     DAZSNG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.242"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..285)
                     /locus_tag="ACHW4Z_004204"
     CDS             complement(<1..285)
                     /locus_tag="ACHW4Z_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8883578.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINT"
     gene            736..1926
                     /locus_tag="ACHW4Z_004205"
     CDS             736..1926
                     /locus_tag="ACHW4Z_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR8883579.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(2316..>2878)
                     /gene="gtrII"
                     /locus_tag="ACHW4Z_004206"
     CDS             complement(2316..>2878)
                     /gene="gtrII"
                     /locus_tag="ACHW4Z_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR8883580.1"
                     /translation="IFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRSIKYLNCMFFL
                     FIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIYIYGSNDSGNM
                     LSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYPIVKSLPSWPN
                     PDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          872 a          551 c          594 g          861 t
ORIGIN      
        1 tgtgtttatt attaatatag aaaatgcaaa agtgaaatat ggaatcatta atctgtaaaa
       61 ctttgacttg atataatcga aaacagataa gtcatttttt ttgatagtaa aaaaaccagc
      121 agcgaaaaag aatagtggga cgtgataact aaaaacaaaa ggatacaatt tgccagcccc
      181 caacccaagg tgacctaaat atatagcaaa aataccgaga aattttaaag catcaaccca
      241 atcatgtctt atttttctgt tgctgtcgat tttgttatta tacatttaaa gtaggctctc
      301 cagttttgtg tgatttttaa acactcattg caaatatgaa tgcctgaaag tttatgatta
      361 ttaatcaacg agttcaatat taatttatta tttttcaaag tcgcactata taggcattta
      421 cttccgcagt accaccgata gtatcgatat caaatcagcc tgcggccgcg ataactttcg
      481 taacattctg ccagcggctt tgttgaataa atcaggttca agataatcgt ctctgcatca
      541 ggttagagtt tcaggcaata catcctcttt ctggcatacc tgccactctc gcaccatggt
      601 catagccttt acgatctgca tactcaccag accagagccc gctccctttt aggctgagga
      661 atccccagaa aagaagacag gcatagagtt ttgtgatcta ctgattgcgc taacaaattc
      721 aatgagatca cctctatgcc tgcccgtaaa gtatgccaga cttttttccg taatgcttta
      781 gcttccacgc atcagtatcg acaaaatgcc attattgatt ctgctgctgc tttagtcggt
      841 ggcgcatctc tttctcttac cagcattggc cggcatttac ccggtcctgc tcgcgtgaaa
      901 gataaaataa aacgcgttga ctgtctgttg ggtaataaac gacttcataa tgatatccct
      961 ctgatattca aaaatattac atccatgatg acaaataaac tgtcgtggtg tgttatcgct
     1021 gttgactgga gcggttaccc ttttcaggaa tatcatgtcc ttcgtgccag cctcctctgc
     1081 gacggtcgct ctatccccct gatgagtcag gtcttcccgt caaaaaaaaa taacgaggcg
     1141 gtggaaatcg cttttctgga tgcgctttcc ggggccattt ctccccggac tcgtgttgtt
     1201 attgttactg atgctggttt tcaaagtgca tggttccgtc atatcaaatc acagggatgg
     1261 gattttattg gtcgtatcag gggggtagtg aagttcaggc tggatagtga caaagataag
     1321 tggctggaca taaaaatgtg cagggggagt tcagaggcaa aatacctggg gacgggaacc
     1381 ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt atctttataa acattcacct
     1441 aaaggcagaa aaagcaggcg tgccagaggg aggccgggct taccaacaac ggaaaaagaa
     1501 caaaaagccg caggcagaga accatggtta atattcagca atacagctga gtttaatgcc
     1561 aaaaaaatca tgaaactgta cagtcgaaga atgcagatag agcaaaactt tcgggatgaa
     1621 aaaagtgaac gctttggttt tggccttcgg gcgagcagaa gtcggagggg agagcgtttt
     1681 ctggtattaa gtctactggt tacactggcc agtatcgtac tctggctact tggttatcat
     1741 tttgaaaata aaggatttca tttaaagtat caggctaata gtctcaaaaa acgaagagta
     1801 ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta accctgaact tctacgacga
     1861 gcacaacctg aaaagatatt gtgccggttg gccagcactt accgaagtat ggtgctggct
     1921 tactgatgaa aattttctgg ggatccctca gcctttcggg gaggaatgag ggctctgata
     1981 ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat ttggcctgag tgacccatgc
     2041 ctcccaaaag cagagtcgct agtgttatgt aattaggcgg ttaaacttca attctcagtg
     2101 ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt tttaattgac taatattaga
     2161 aacggttagt gtgattgtat atagatattt gagtgtaagg tatttaaagg ggcattcagc
     2221 ccctccgtct atttgtgatt gcatccattg atgtgtgtat tattttgagc ttgaaaaagg
     2281 gaggcgctct ttaataataa ataaatctat caaagctaaa tattaaatgg aagccaccct
     2341 tttttctctg atagttttat tatcaccaaa ccatttattt cagctatcga atctggattc
     2401 ggccaggaag gtaaactctt cacaattgga taaatcttct ctatttgttc tttatttgct
     2461 ggtttgcaat tacagattcc atagtaattc ataaatgcaa ccatcctaaa atagttgcca
     2521 ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc tcatggagag catatttcct
     2581 gagtcgtttg aaccatatat atatatatat tttccgttaa gggaaatgcc ttttgtttga
     2641 gatgtgtatg atatttcttt agctaaagag atatctttct gtcttgcgag agtatcattc
     2701 aaaaatagat ttttggatgt tgaaacgcca ttaaatataa taaataaaaa aaacatgcag
     2761 tttaggtatt ttatagagcg aaaattgctt aagcaagaaa acaaaattac tgctactata
     2821 ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa caacaacgat aaatataa
//
LOCUS       DAZSNG010000239         2872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZSNG010000239 DAZSNG010000000
VERSION     DAZSNG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.243"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..613)
                     /locus_tag="ACHW4Z_004207"
     CDS             complement(<1..613)
                     /locus_tag="ACHW4Z_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8883581.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVMLPT"
     gene            complement(680..1405)
                     /gene="fimC"
                     /locus_tag="ACHW4Z_004208"
     CDS             complement(680..1405)
                     /gene="fimC"
                     /locus_tag="ACHW4Z_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8883582.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1442..1981)
                     /locus_tag="ACHW4Z_004209"
     CDS             complement(1442..1981)
                     /locus_tag="ACHW4Z_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8883583.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="ACHW4Z_004210"
     CDS             complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="ACHW4Z_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8883584.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          747 a          715 c          632 g          778 t
ORIGIN      
        1 aagttggcag cattacccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
       61 cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg cgattactca
      121 taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag
      181 cgtcctggac cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg
      241 cgacagaagc cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa
      301 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
      361 tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
      421 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg
      481 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac
      541 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
      601 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
      661 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
      721 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
      781 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
      841 ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa
      901 atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
      961 gtttaatgcg gctgataatt gcgagctgta gcatattctc agtcaatttt gatttatcca
     1021 ttgacggaat cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt
     1081 tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag
     1141 gaggcgtcac gataaaacga ccatccttta caccatcggc attttccacc catgattgaa
     1201 ttaaataggt actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg
     1261 ccggataaat tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca
     1321 tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc
     1381 ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca
     1441 attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac
     1501 gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt
     1561 tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta
     1621 caccaatatt ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac
     1681 catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc
     1741 gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac
     1801 tgctgatttg ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt
     1861 ccgcaataat gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat
     1921 ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca
     1981 tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg
     2041 gtaggttatt gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc
     2101 tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt
     2161 gtttgtgcac taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtctaggatc
     2221 tgcacgccaa cgtttgttgc actacctgca gcagaactct gcagagccag tacatcagta
     2281 cgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta
     2341 tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc
     2401 aggctagcgg tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact
     2461 gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg tcccaccatt taccgtcgta
     2521 gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga
     2581 gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc
     2641 gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca
     2701 aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt
     2761 tcagtttatt tgttggctta atattctatt attatcttta tttatagatt tttatattgc
     2821 atgaggtggt ttttggagag aagaatgagg aagatgcgtc gagccacaga aa
//
LOCUS       DAZSNG010000240         2845 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZSNG010000240 DAZSNG010000000
VERSION     DAZSNG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.244"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..206
                     /locus_tag="ACHW4Z_004211"
     CDS             <1..206
                     /locus_tag="ACHW4Z_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883585.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="ACHW4Z_004212"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="ACHW4Z_004212"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR8883586.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2105..2821
                     /locus_tag="ACHW4Z_004213"
     CDS             2105..2821
                     /locus_tag="ACHW4Z_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8883587.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          788 a          626 c          632 g          799 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat
     1981 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2041 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2101 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2161 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
     2221 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2281 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2341 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
     2401 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2461 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
     2521 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
     2581 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa
     2641 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2701 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2761 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2821 attatatttt aaagaaatct gtctt
//
LOCUS       DAZSNG010000241         2829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZSNG010000241 DAZSNG010000000
VERSION     DAZSNG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.245"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1647)
                     /locus_tag="ACHW4Z_004214"
     CDS             complement(<1..1647)
                     /locus_tag="ACHW4Z_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR8883588.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQ"
     gene            complement(2079..2798)
                     /locus_tag="ACHW4Z_004215"
     CDS             complement(2079..2798)
                     /locus_tag="ACHW4Z_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR8883589.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          866 a          546 c          473 g          944 t
ORIGIN      
        1 ttgttctgct atagtatccg gttccagatt gttctctttt attattctac aatactctaa
       61 tattgtttct ttgtctctat ttactacaga tacaaataca ccaggaatac catctttatt
      121 agttgcggac agcatttcga ccaatcgtct tgtggtaaaa ctatagtttt ttagaatatt
      181 acaaaaggca cgtattgttt cggtatggcc attataaaat gccataaaaa gtcctgatgt
      241 accgttggga ttttttatcc ataataattc atctatatat tcctggtgag gggggagtat
      301 ttttagtata tctccatacg ccataatggc ctttgtgtgc ccattttgaa gtgccataaa
      361 caacccatgc tttactttca ataattcttt aatttgttca ctactaagca ttttcttatt
      421 aagcagactt ccgaacatac gaatactatc tgcatgtcca ttctgcaatg caaaaaataa
      481 cccaggtaaa tcatggctgt ctttcgcagc taatagatat aatctcttat cggaagttaa
      541 cggggtttta aagactattt ccccataggc ttttattgtg tctacatgtc cgttcataaa
      601 agacatgcat aaacccggca cattgtttgc ccctaccgca gcaagcaatg taagtattgt
      661 ttcttgacta agattgcaca tatttaatat cttaccatat acttgaataa tgtctgaata
      721 tccgttttga agtgctatat aaaatgcagg agtaccatca gagcttctcg tggccagaag
      781 gttcaccagt ttagattgag aaatttcacc atttttgtat ttttccatca gtataaataa
      841 tacttctgtc agattctttg ttatcgcatg atatataact ctggaattga tcaattcatc
      901 tggtaaaagt ttcactgtgt ttgttagagg ggaattatgc ctatcttcaa atattacctg
      961 cccgggttgc tccagaccat aaattttcca ataaaaatca tcaaaaaaat ccttagcgga
     1021 taattgactc agttcgaaat ggctatcaca agtaaataca gtacgtttat gagtaacagt
     1081 tcgattagga tcgaaaaact gaacaaccca tttttcactt tcttgtacgc gctttatttt
     1141 caaccctaac gccatttggt ggtttaatgt gcatgctgat aatgttctga caaagtctcc
     1201 attttttttc atgttataaa aaaaattagt aataactgtt ccccactttt tattttccac
     1261 aaaataaata taagctggtg attttgaaaa atcttccgtt ttttccaaac atacattttt
     1321 tgcaatagca tcaggggtgc taagaagctg atagtttacc ttcccatttg tttccataaa
     1381 attcatttcc caataggaag ctaggtgacg gcaaactatt tgtcttccat atatatcttt
     1441 tgccactcca tttatattta cctccacatt tctcttttct gaaaaataag gtgctgtttt
     1501 attcttaaac tgaattagtt cactggagtt tgagaccctc ataatagaac ttgttattgt
     1561 tctattatct ctgttaacag acatatctgt tgataacgaa ttttttgttg aaaatatact
     1621 agatgaaaat gttttattta acggcataga agacaaccat agagatgtta taatactatt
     1681 agattgcagt tactaatagt aaaagcctcc ttacaaggag gtagcttatt cagaatgctc
     1741 ctataagata gcgagctcgt caacgataca gatgatctga ttatattgtg caaaaggaga
     1801 acaggaaaat aggcactgga aaagatgaag gcctgatggg agcattaact aatgcaaaat
     1861 ccgacaggtt aatatcccga aaagcaatca aacgactcaa acaggagatg cacgctgaaa
     1921 tggtggacaa ctgttcggat ttcagatgag tagctcagca gtttatgagc cgaatgctct
     1981 gtcacgggca ggaatcaggc tgcatacgca gtgccaaatc taatcgtagt tattctaacc
     2041 agttttcaag ctacaaggtg gtgtagctgg catcttctct actctatcat caaacgataa
     2101 aatggttcct ctcgtaacat ttgctcttgc ctttcacttc catctcgatc gcttcgtagt
     2161 tcattacgat agctgacata tttccagtct tcaggtctaa catctgacgc cggatactcc
     2221 ccgggagcga ttttgcttct taatagatta gactcgagac acattataaa ttgtcgtatt
     2281 ttatgaagta ataaagcact atattgcgag cattcctgat ctgattttac atatagcgta
     2341 aactgagctc ctatccccac acgagattgc tgagaaactc gattcatatc agttatcttc
     2401 catttatcta taggactgtc ctctgaaaat aataaaccag acaaaatttg gaatgccaga
     2461 ggaacttgct ccctagctat actaatatga aacttgtccc caacaaaatc ccccttactc
     2521 tgaggatttt cacgacatgc atgaataaac acatctccac tatggttcac aatctggaat
     2581 ccttggtaca tgacagaaaa agattgacat atatcagagt aagttggcac gttcgatgct
     2641 actttgaaat agtacgctgg gtattgccgt atttggttgt acaatatatt gaagttattt
     2701 ttcaatcttt cattcgcaga aagcatctgc ggtatttcag acctcacaac attcaaagaa
     2761 tctaaattta gttttagaca gggctttttt atgggcatag aaaacgtcct ctataaaata
     2821 gatatatta
//
LOCUS       DAZSNG010000242         2815 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZSNG010000242 DAZSNG010000000
VERSION     DAZSNG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.246"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..1941)
                     /gene="mobF"
                     /locus_tag="ACHW4Z_004216"
                     /pseudo
     CDS             complement(1..1941)
                     /gene="mobF"
                     /locus_tag="ACHW4Z_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
     gene            2023..2250
                     /gene="vapB"
                     /locus_tag="ACHW4Z_004217"
     CDS             2023..2250
                     /gene="vapB"
                     /locus_tag="ACHW4Z_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR8883590.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            2250..2648
                     /locus_tag="ACHW4Z_004218"
     CDS             2250..2648
                     /locus_tag="ACHW4Z_004218"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR8883591.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          585 a          871 c          710 g          649 t
ORIGIN      
        1 cccgggcggt acatatcccg cagataacgg ctccggctgt ccagccagac cggtgacagg
       61 gcggtcatgg ttacctccgg gtgtccgagc acgccctgtg ttttcagctc actgcgaatg
      121 gcctgtgtca gtatggcctg ttcccgtact ccgctgacct gtgccacgct ctcttctccg
      181 gctttcacgc tggccgcaaa atctccggcc agccgacatt acggtccggt tcactgatga
      241 tggtggccgg tcgctgttct cccccctgcc agcgatatgt gttcaccccg gcatccttca
      301 tggccatcag tgcactgcct gtaccggttc gctgcccgct gtcggttatc aggacctgta
      361 cgttatgacg tgcggcacca tccagcaggg ttaacgtctc tttcagggag agtttttcgc
      421 cctggtcgac gataacggta ctgcccggcg tgaaggccat tccttccagc aactgacgac
      481 gtccggttat cagttcaccg gacagccgtt catcctgctt caggttcatc tgcgagcgac
      541 ggtcagcagc gataatctgc acctcccgcc cctgctcccg ggccatcatg accagttcag
      601 ccacccgctc acgctgcccg gctgcaccgc cctgcccgga cacaatggcc agcgacggac
      661 ggtcctgtgc cagaacactg acggcatcgc tgtaaccggc cgtccggggg acacttttct
      721 ccggatgtac ggtcacccgg ttctgtttca tgatgtcacg actgagtgcc cggactgaca
      781 gctcatcgag catatgaatc ccgaacgtga acagcccctt ctcacgatcg agggggataa
      841 gctgctcacg gctgatggcc tcatcgatac cggcgcgtgc ccgttcaatc acaccatttt
      901 ccggcggcag tatgccgacc gtcctggcca gcaggtccgt atacataaac tgcactttgc
      961 gttcacttaa tccggcaatg gcctgtgtca ccgcctgctg cacatccggc ccgtcctgtg
     1021 aagcgggtcc gggtgtctgc atgcgggtat atgcccgttg ttccgctgcg tcacgatatg
     1081 cccggatgtc gaacccggtt tccttcagcg tctgcatcca ctcggccatt ctgacttcag
     1141 gatcgacgtg ctgtttggat ttacgcgtat ccagcgccgc cacatcccgg gatttcagcg
     1201 aggcgtcttc cccgacggcc tcccggatag tctgtgagcg tccggaaaag gcctccaccg
     1261 gtacacccgg catttcccac ataccgtgct taccgaccac ttcagtttca tagcccagcg
     1321 cctcaacctg ctctttcagt ttttcccggt agagcctgcc aaaggcaatc tgattagcgt
     1381 acacattctc aatgaacccc gttttcccca ctttgtcact gctcagtgtc ttccattcgc
     1441 cgttatactg cgtaacatta gtcaccaccg catgcgtgtg taactgtggt tcctgatcgc
     1501 gactggtgtc gtggttaaac agtgccatca ccagattacc ggtcagcacc gtttctgact
     1561 gtccgtccgt caggtggagg cactggcctc cacctgacgg acagcaaaat ccacggcctg
     1621 gttatgtgct tcaataagac gtttgtcccc cccgagcatg gccatcatgg agatactttt
     1681 gggagcggag aaggtcaggt cgtagccggg acgatgcctg ttactgccat cctgcatgcg
     1741 gcttaaatcc gccccgtccg gcagcctgcc ctccagaagg cgggtaaaga catccttatc
     1801 gacactgccc tgcagcccca gttgttcagc cccctggccg gcccagcgtt ctcccatgct
     1861 gcccagcaca tagtaattat ccttgtcggt ataataatta ccggcacttc cggccgatct
     1921 gacctgcgcg atactcatca taaacgtata tccctttgac atatcccggt atcaatccca
     1981 caatagatat acacaagaca tatccacata aggaggcaaa taatggaaac caccgtattt
     2041 ctcagcaacc gcagccaggc ggtcagactg ccaaaagcgg ttgcattgcc ggaaaacgta
     2101 aaacgcgttg aagtgattgc tgtcggacga accagaatca ttacgccagc cggagagaca
     2161 tgggacgaat ggttcgacgg acacagcgtc agcaccgatt ttatggataa cagggaacag
     2221 cccggcatgc aagaaaggga gtcattctga tgctgaagtt tatgctcgat accaacatct
     2281 gcatttttac gataaagaac aaacccgcca gcgtcaggga gcgttttaac ctgaaccagg
     2341 ggaaaatgtg tatcagttcg gtcaccctga tggagctgat atatggtgca gaaaaaagcc
     2401 agatgcctga acgtaatctc gctgtgatcg agggatttgt ttcccgcatt gatgttctgg
     2461 attacgacgc tgctgcagcc acacacaccg gccagataag agcagaactt gcccgtcagg
     2521 gacgccctgt cgggccattt gatcaaatga tcgcaggtca tgcccgcagt cgggggctga
     2581 ttattgtgac taataacacc cgggaatttg aacgtgtggg cggcctgaga actgaagact
     2641 ggagctgacc tgttcatcag aaatcatctc ccggctcaac atcctccccg cgttcccggt
     2701 gcacattgat gttcacctct tcacgacgct gcatctgctg ccaggtgtcc ggatgatttt
     2761 cctgctgcca tgcctcataa gcggccatat ccaccacttc accggattca ctgat
//
LOCUS       DAZSNG010000243         2807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZSNG010000243 DAZSNG010000000
VERSION     DAZSNG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.247"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..81
                     /locus_tag="ACHW4Z_004219"
     tRNA            6..81
                     /locus_tag="ACHW4Z_004219"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHW4Z_004220"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHW4Z_004220"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHW4Z_004221"
     CDS             complement(299..1057)
                     /locus_tag="ACHW4Z_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR8883592.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHW4Z_004222"
     CDS             complement(1065..2168)
                     /locus_tag="ACHW4Z_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR8883593.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHW4Z_004223"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHW4Z_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR8883594.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZSNG010000244         2792 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZSNG010000244 DAZSNG010000000
VERSION     DAZSNG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.248"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..156)
                     /locus_tag="ACHW4Z_004224"
     CDS             complement(<1..156)
                     /locus_tag="ACHW4Z_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OspB"
                     /protein_id="HFR8883595.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGF"
     gene            complement(406..525)
                     /locus_tag="ACHW4Z_004225"
                     /pseudo
     CDS             complement(406..525)
                     /locus_tag="ACHW4Z_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            769..1141
                     /locus_tag="ACHW4Z_004226"
                     /pseudo
     CDS             769..1141
                     /locus_tag="ACHW4Z_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1703..2650)
                     /gene="icsP"
                     /locus_tag="ACHW4Z_004227"
     CDS             complement(1703..2650)
                     /gene="icsP"
                     /locus_tag="ACHW4Z_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR8883596.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          813 a          587 c          542 g          850 t
ORIGIN      
        1 aaaacctttc tctcctaaaa aaaccaatgc tgcttttggg tgagtacatg atgaattttt
       61 tacccttttt attatatgtg caaatgcaat atctgatatg ctttcattct ttttattgac
      121 tattctacag tatggtctaa caccatctaa attcatacct acatgactcc atatattgtt
      181 ttataaacat catagtaaag ggtgttatat tttcatatag tatgcaagct tctttgtata
      241 ctatgattta aaaaaacgga acaaaaatgc aattatattc tggcattgtt taatgcggtt
      301 tgtctttttt attcattatt tcaatgaaag aatttcaact acaaagttaa ttgatcctac
      361 ctacataata tagacacagt acctaaagta ccgtggagat catccgggga agccccgggt
      421 ctgataccag tgcaggtaga ccgctatctt gttccggtaa tgttctgccg ttctttacgg
      481 catcacctct agcagaaaca tcgcgtaact gcgccaagta tgatgaactt ctcttaccgc
      541 tgcgatctgg ttcaacaata tgcagatggt acacaacctc tttaaccagc ccatccggat
      601 tattcagagc cgcttccgca atccggaata aaagtgctgt tttaccgtga accctctgaa
      661 aatcagcgag cagtgtttta accagccgac gttcagagcg tactgacaga cggtggacaa
      721 tttgaataaa cagttcaatc agaccgtaga tgctctcccg ttggcgacat gctgggagcg
      781 gtggaacacc gcttcggcaa cgagcttccg gcgtctccag tgaagtgact gacggataat
      841 ggttcatgct accgagctaa tgaaacacgc cagttcaccc ggatgttggg actttgttga
      901 accgaagaac acggcggtgc ggagtccgga gagtaacaga atagcaaaga gcttcgtgaa
      961 aacaataaag tgtgactaca tcagtatcat gcccaaacct gacgggttaa cggcagcaaa
     1021 gaaccttgca gaggcattcg agcattataa cgaatggcat ccgcatagtg cgctggatta
     1081 tcgctcgcca cgggaatatc tgcggcagcg ggccaatgat aacaggtgtc tggaaatata
     1141 ggggcaaagc cacccacgaa cgatccagca tataatcaag aaattagatt tcatttaaca
     1201 taagatagat tatgtacacc aagaggcgtt tccttaattt ctgaatttac aggctctttt
     1261 gctctctttg ctatgctgtg gatttggaaa gatagagtgc tggctgatta tttaggtatt
     1321 ttcatcagca tgtccctgat agcactgttc catcagcgct ttcaggcgat aatagtagac
     1381 cttgggtagc ctctgcccat gtgtaacttg ataccccgag aacagccacg atgatctaag
     1441 cggttcttat ttctccggtc tcagggtcgg taatgccaac agtaagtcca cagtaatcaa
     1501 caaagagttt tttgaaccgc cccggaaatc ctggagacta aacgccctga gaaagaggta
     1561 aacagaatga ctaaaaatac acgtttttcc cccgaagtcc gtcaacgggc agttcgtatg
     1621 gttctggaaa gtcagggcga atatgactca caatgggcgg caatttttag ccatgaaaac
     1681 gtaatcaact cggtgaacca attcaaaaaa tatactttat acctgcggaa gtgagaaaac
     1741 caatatattc aatacttgca ctatttttaa tggtgccaga tatattggta ctttgctcat
     1801 aaagagatgt atcacctttt ttattagaaa tgtaatttct ggagccttct atataaaatt
     1861 ttgcactggg ggtgacatag tatccaatat tcgcagcaac agagcagtaa ttttggtttt
     1921 ttatttcatc tttaaaaatt gtctcagtct gatagtgttt atctgtatca gatgaaagaa
     1981 cccaaccact atatttcagt tctgctccaa actcaaaatt ctcatggcgg taattagcag
     2041 ttaatccaat ataaggtatt ttgaagagct gtttgtaacc tattgttctt tcaccactag
     2101 gatgtgcccc ttttttattc ctgctccccc cgttttcact ataaatataa ctccctccca
     2161 ttgcattaaa actgtaacga ctctcttggt aacctgctat tagtccaagt cgataatcca
     2221 aattatttaa taaccaacct ttaagattca gctcgaattc gttagcatca cggagatgcg
     2281 tattgggatg ccagctctgg tcggtccata cctgaggggt attggaattg ttccagtctt
     2341 tatcaaccat gcttgctttc tggttaccta aagtagtcca accggaaact ccgaaagaca
     2401 cttttggaat tagcttccaa tcgatgccac ctctaacaat agtcgcatta ctgtatttcc
     2461 agtccagttg actaattttt ctcccccctt ccttgggatg ataaacgcgt tcttttgttt
     2521 tgccactcag agatcctaga ctaatatccg tactgatgtt gtccggtatg aaaagtggat
     2581 agttagtggt agcatgtgta gtaaagatcg caggtacaca aagtgcaagt acaaagaatt
     2641 ttaatttcat cgagaactct acttttttgg ttgaaatgtc catgatcttc ttactttata
     2701 agataaacgt caatgccaag cattatttta attaaatatt tgtttatgtt accaagaaca
     2761 tatcttttat tataatattt attgaacttt tg
//
LOCUS       DAZSNG010000245         2765 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZSNG010000245 DAZSNG010000000
VERSION     DAZSNG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.249"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2645
                     /locus_tag="ACHW4Z_004228"
     rRNA            <1..2645
                     /locus_tag="ACHW4Z_004228"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          721 a          604 c          872 g          568 t
ORIGIN      
        1 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
       61 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      121 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      181 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      241 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      301 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      361 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      421 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      481 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      541 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      601 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      661 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      721 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      781 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      841 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
      901 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
      961 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1021 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1081 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1141 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1201 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1261 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1321 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1381 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1441 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1501 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1561 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1621 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     1681 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     1741 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     1801 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     1861 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     1921 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     1981 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2041 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2101 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2161 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2221 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2281 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2341 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2401 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2461 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2521 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2581 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     2641 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     2701 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     2761 ggtcc
//
LOCUS       DAZSNG010000246         2738 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZSNG010000246 DAZSNG010000000
VERSION     DAZSNG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.250"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..681
                     /gene="ospC1"
                     /locus_tag="ACHW4Z_004229"
     CDS             <1..681
                     /gene="ospC1"
                     /locus_tag="ACHW4Z_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR8883597.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYG
                     ATSDNKYI"
     gene            complement(785..934)
                     /locus_tag="ACHW4Z_004230"
                     /pseudo
     CDS             complement(785..934)
                     /locus_tag="ACHW4Z_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1010..2707
                     /gene="ospD3"
                     /locus_tag="ACHW4Z_004231"
     CDS             1010..2707
                     /gene="ospD3"
                     /locus_tag="ACHW4Z_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR8883598.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYISVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          973 a          449 c          521 g          795 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat
      601 aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca
      661 tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca
      721 caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt
      781 tgatgggttt cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac
      841 agcgatagag cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag
      901 agtaacggtg acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca
      961 acaacactaa gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt
     1021 aaatttaatc ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc
     1081 tgttgagaca atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag
     1141 gagtttttta ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg
     1201 tcgacattta gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta
     1261 tcatcacttt gcttttccgg atgaaattaa aaattatatt tcagtgagtg aagaagaaaa
     1321 ggctattaat gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat
     1381 tttttatatt ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag
     1441 tacatcaaat cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga
     1501 ttttgtcatt cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa
     1561 caaatttaac ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca
     1621 tcagaaatgt tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc
     1681 aacaagcatg tcctccataa tcagatggcc tgataattta cttcacccca aagttattta
     1741 tcacgcgatg cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact
     1801 atctgacctt tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt
     1861 gtcaggattg cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga
     1921 actcttggaa acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg
     1981 aatgggagga cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat
     2041 caaaacatac ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa
     2101 aaatttgctg accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt
     2161 tgctcttcaa aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc
     2221 ccctctcctc aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt
     2281 tcccggactt ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga
     2341 tatcttgaat gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga
     2401 ttctaatggt ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat
     2461 tgcttatggg aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact
     2521 ggcagcagaa ggcccaaatg gggtatctgg attaattata gcttttcaaa ataggaattt
     2581 tgaggcaata aaaacttata tggaaataat aaaaaatgaa aatattacac ctgaagaaat
     2641 agcagaacac ttggacaaaa aaaatggaag tgattttcta gaaattatga agaatataaa
     2701 aagctgaata ttatctcgtg gtgatacact gaatttga
//
LOCUS       DAZSNG010000247         2716 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZSNG010000247 DAZSNG010000000
VERSION     DAZSNG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.251"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..224
                     /locus_tag="ACHW4Z_004232"
     CDS             6..224
                     /locus_tag="ACHW4Z_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR8883599.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="ACHW4Z_004233"
     CDS             226..591
                     /locus_tag="ACHW4Z_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8883600.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="ACHW4Z_004234"
     CDS             588..1253
                     /locus_tag="ACHW4Z_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883601.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="ACHW4Z_004235"
     CDS             1253..1618
                     /locus_tag="ACHW4Z_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR8883602.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="ACHW4Z_004236"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="ACHW4Z_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8883603.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2504..2716
                     /locus_tag="ACHW4Z_004237"
     CDS             2504..2716
                     /locus_tag="ACHW4Z_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883604.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          772 a          557 c          697 g          690 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgttag
//
LOCUS       DAZSNG010000248         2716 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZSNG010000248 DAZSNG010000000
VERSION     DAZSNG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.252"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(337..1116)
                     /gene="ipaJ"
                     /locus_tag="ACHW4Z_004238"
     CDS             complement(337..1116)
                     /gene="ipaJ"
                     /locus_tag="ACHW4Z_004238"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR8883605.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1313..2122)
                     /locus_tag="ACHW4Z_004239"
                     /pseudo
     CDS             complement(1313..2122)
                     /locus_tag="ACHW4Z_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2469..>2716
                     /locus_tag="ACHW4Z_004240"
     CDS             2469..>2716
                     /locus_tag="ACHW4Z_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883606.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          811 a          680 c          442 g          783 t
ORIGIN      
        1 tcatagggct tacagtttaa attgcgggta taaaatacat ctattttcaa ataaaataaa
       61 gcatactcca attacaacga gtgactaaaa taaaaatatc atccaagtca atcactgttt
      121 gctctgcaaa atatgtcagc cttctcaaac ttaccagcac ttaaatattt aagcacacaa
      181 tcattccttg agggagttca cttatttcaa gagaaacatt tcgtcttccc acacaactaa
      241 tccgcccaat atagctaaat ttgtactcag taatatcatt tgttatgccc cccaccccaa
      301 aatattcaag ctccttcaca gataaaaaac gacaatttac aaagcctcat tagttataac
      361 tatggaaata cctattttag tatatcctat aaagttagaa ttacttcttc ttgcacctaa
      421 ttccatagtt gaaaaacaag aataattttc tcctacagct ggatccatat agcttccatc
      481 aggtctacac aacacccagt ggaggccgac aggaactcca ataaaggata cagcaacagc
      541 ttccaaaact ctttggttag aagataacac atctcgttga tgtactatat tacatcccat
      601 gccaattaat aagtcccttg catcgggata aataaaacta attacttgcg aaaaaatgtt
      661 actttcttca acgacataag cattcaatcc aagaagcctc gtagccatga ctatcttatc
      721 aggcatagaa taaccggcat ccgcgatatt atctttgtgt attctcggat tataattacc
      781 actagttatt aagtataaat cccgctcaca acgattatcc aaatcaagag aactcttcct
      841 tgtcatttcg gacatagacc ctttgtattg tggtatttta tcaaccccta attcctttgc
      901 agcacagagt aaactggcag ctccacaaga attgtcatac atctgcttcc tcgccaaaaa
      961 acaaccttgt tcgtttactc tattctcatt taccctcaca tcagtctgat gagatatcat
     1021 atattctctt ggtatagccc cctgcaataa aaccccatat aacataacac ctgtatgaat
     1081 acatccccgt ttgcaaggct tccgttgttc cgacatcgac gagtaattgc agcacagtat
     1141 tgcatccagc aatgcgaact actatgagaa ttagctgacc gggtccttat tccccgaacg
     1201 aactttaaat ccaaaacctc atactctttt ccgtcgtcat cacgtccgtc accataacaa
     1261 cctttcagca tccacccttt tattaagagt taatgtccat cagcatcagc aattaatacc
     1321 gcctgatact gacatatttt tgtcgtattt acgtccataa tcggttattg tgatacaggt
     1381 cacaccagct tatttgctat tgttacaata accaccttgg ttgagagctg ttttttcaac
     1441 tttgttatca actcccccaa ctccttggtc agtaaggctg ccgaccgagc acagtgaata
     1501 gatagtatcc gcaggtacca ccatgcttac ttaatactca gaaggcgaag tgctctccaa
     1561 ttaactctgc cgcactatgc ttataagctg cttttcaatg ccgtctatca acatgtccaa
     1621 ttcattcaat ctgttgtact ggtaatccaa cgcagtaatg atatatgcag gtattctttc
     1681 atccactcca gtaacgtttc atgcaaagca ttaatttacg cgctacggaa cttcaccagt
     1741 tgactgagca aacgatgtgg gaccagtact gcctgcagtt cttctattgt tacagcgatt
     1801 tctttaccaa acagctgaac caccatcaag ataaagatag cctgaacatt catcgcacca
     1861 ttttgttacc aatggcaaaa gatttaacga accgtgcctg catcaggcgg actttttatc
     1921 cctgctttgt tagctcccta accttgtaca gagagcctcc ataagctccc atatcaagca
     1981 gacatggttc gcagttactg acatacacca gaaaactctg acagcagact cacttatcct
     2041 ccacttcact catatcctta ttggtgagat gaacctgaat gacatgtttt gcgacataaa
     2101 cgccaaccag tgtatatttc acctgtagag attctacaat ctgtagcgtt atgcatctaa
     2161 tgttaggctc tatgatgtca gaaatctgcg agactctaca ccttctgcat ttacctttat
     2221 ttatcaccat gctcctcagt taacggagaa cgtgttcatt gcattttgtc acttacattt
     2281 cgcctttggt atcgggggga tataggttcc aacgttccgc atgagaacct gaaccggctt
     2341 cacgccacct tgataccaga tgccgcacag gtagtcaaca agtgtcacga acggtgcaat
     2401 agtgatccac acccaacgcc tgaaatcaga tccagggggt aatctgctct cctgattcag
     2461 gagagcttat ggtcactttt gagacagtta tggaaattaa aatcctgcac aagcagggaa
     2521 tgagtagccg gacgattgcc agagaactgg ggctctcccg caataccgtt aaacgttatt
     2581 tgcaggcaaa atctgagccg ccaaaatata cgccgcgacc tgctgttgct tcactcctgg
     2641 atgaataccg ggattatatt cgtcaacgca tcgccgatgc tcatccttac aaaatcccgg
     2701 caacggtaat cgctcg
//
LOCUS       DAZSNG010000249         2712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZSNG010000249 DAZSNG010000000
VERSION     DAZSNG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.253"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHW4Z_004241"
     CDS             50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHW4Z_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR8883607.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2712
                     /locus_tag="ACHW4Z_004242"
     CDS             2583..>2712
                     /locus_tag="ACHW4Z_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8883608.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          678 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc gg
//
LOCUS       DAZSNG010000250         2686 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZSNG010000250 DAZSNG010000000
VERSION     DAZSNG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.254"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..358
                     /locus_tag="ACHW4Z_004243"
     CDS             <1..358
                     /locus_tag="ACHW4Z_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8883609.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            620..982
                     /locus_tag="ACHW4Z_004244"
     CDS             620..982
                     /locus_tag="ACHW4Z_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR8883610.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            979..1908
                     /locus_tag="ACHW4Z_004245"
     CDS             979..1908
                     /locus_tag="ACHW4Z_004245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR8883611.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1905..>2686
                     /gene="gtrII"
                     /locus_tag="ACHW4Z_004246"
     CDS             1905..>2686
                     /gene="gtrII"
                     /locus_tag="ACHW4Z_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR8883612.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGL"
BASE COUNT          779 a          533 c          550 g          824 t
ORIGIN      
        1 ggtgtttgtc cacaccaaac ctgcctaccg aagcgacgga acaaaaacag cagcggtaag
       61 gaagatgaga accgacagca acaaggcatg gaagggagcg ttaaagcggg caggcattag
      121 caacttccgc ttccatgacc tgaggcatac ctgggcaagc tggctggttc agtccggtgt
      181 ctctcttctt gcgcttaaag agatgggagg atgggaaact ctcgaaatgg ttcaaagata
      241 cgcccacctt tcagccgggc atcttaccga gcacgcgagc aaaatcgatg cgattataag
      301 tcgcaatggc acaaatacgg cacaagagga gaacgtagtt tacttaaatg cgaggtaact
      361 tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca ttacgaatta
      421 taagaatccg cttgtaattc aaaggattat cccatcaaca ctgcgctcac acgtcccacc
      481 acatcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc ttatgtgtct cagttttgtc
      541 tcatcagaca tagcaagtat cgatcaattg agacttggat gatagacttc atgcctttca
      601 gagctcattg attaaataaa tgttaaagtt atttgtaaag tacacatcta taggtgtgct
      661 gaacacactt atacactggg tggtttttgg tgtatgtatc tatgccgcac ataccagtca
      721 ggctctggca aacttcacag gtttcgtagt ggctgtgagc tttagcttct tcgcgaatgc
      781 aagattcaca ttcaaagcat tgactacagc gatgcgctac atgttatacg tgggatttat
      841 gggaatattg agtgtgattg ttggttgggc agctgataaa tgctcacttc ctccaatagt
      901 cactcttatc accttctccg ccatcagtct ggtgtgcggt ttcgtctatt caaagttcat
      961 tgtctttagg gatgcgaaat gaaaatatct cttgtcgttc ctgtcttcaa tgaagaagaa
     1021 gcgatacctg ttttctataa aacggtacgt gaattccaag agttgaagcc atatgaagta
     1081 gaaattgtat tcataaatga cggaagtaaa gatgccacag agtcaattat taacgcgctg
     1141 gctgtttcag acccgctagt tgttccgctg tcatttacac gcaactttgg taaagaacca
     1201 gccttatttg cagggttaga ccatgcaagc ggcgatgctg taattcctat tgatgtcgac
     1261 ctgcaagacc caattgaggt tatccctcat cttattgaaa agtggcaggc aggtgctgac
     1321 atggtgcttg ctaaacgttc agaccgctca actgatggac gactgaaacg taagacagct
     1381 gagtggttct ataaattaca caacaaaata agcaccccaa agatcgagga aaatgtcgga
     1441 gattttcgac tcatgtctcg tgaggttgtg gagaacatta aactgttgcc tgagcgcaat
     1501 cttttcatga aaggcatact gagctgggtg ggtggtccga cggatgtcgt tgaatatgta
     1561 cgcgcagagc gtgttgctgg catctcaaaa tttaatggct ggaaattatg gaatctggca
     1621 ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg tatggactta tataggcttg
     1681 tttgttgcaa gcatttcatt tttatatggt gcatggatga ttatagacac ccttgtcttt
     1741 ggtaacccag tacgcgggta tccctccctg cttgtatcaa tacttttctt gggtggagtg
     1801 caactgatcg ggattggtgt tctcggagaa tatataggta gaatctatat tgaagtaaaa
     1861 aatagaccca aatacatcat aaaaaaatct catcgaggta acccatgatt aaaattaatt
     1921 tatttaaaaa tgcaaatctc cttgccttca tatcatgttt tgcaatatca atttattgtt
     1981 attgggggtg gttgtacgat ggaacactta atattgatgg cgagtttaca aataattttt
     2041 atcaaacaat cacgcttggg cggtggtttc acactttttt gcgacattac tttctccctg
     2101 agcctttttc actttatata acaccattaa tagccttatc ttttatcatc atttcagcat
     2161 ttataatctg cagatcgcta aagctagaat cttatgaatt attgataggt atgttagtat
     2221 ttattacctt ccctcagatc tcctatcaat tagagtttct taaccaagct gatactgtgg
     2281 gaattgcttt tctactggca gcgatatcag caattatttt tcactcgcaa aaaaatagga
     2341 ttgtgatatt ttctggtata gtactgtcca ttctttcaat ggcaatctac caaacattcg
     2401 taacatatat tattgcattc gtcattgggt tgcagataaa ttcgataata cgaaatgaga
     2461 aaaatattcg tgaatctttt tatagttcat gtttatctct atccctcata gctttatcta
     2521 ccttaattta cctgctatta accaaagcta tcaagcatta tttttcgctt gaatcgaacg
     2581 agtacatctc aaattatata caaaatgcaa gcgatattaa atggcttgtt aaatcagcca
     2641 tagataatat atataacttc tataacaatc ctcccactgg tttaaa
//
LOCUS       DAZSNG010000251         2655 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZSNG010000251 DAZSNG010000000
VERSION     DAZSNG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.255"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..224
                     /locus_tag="ACHW4Z_004247"
     CDS             <1..224
                     /locus_tag="ACHW4Z_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8883613.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHW4Z_004248"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHW4Z_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR8883614.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHW4Z_004249"
     CDS             518..1108
                     /locus_tag="ACHW4Z_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8883615.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHW4Z_004250"
     CDS             1348..1608
                     /locus_tag="ACHW4Z_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8883616.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHW4Z_004251"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHW4Z_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8883617.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..>2655
                     /gene="repA"
                     /locus_tag="ACHW4Z_004252"
     CDS             1899..>2655
                     /gene="repA"
                     /locus_tag="ACHW4Z_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8883618.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTA"
BASE COUNT          711 a          606 c          697 g          641 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgcca
//
LOCUS       DAZSNG010000252         2629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZSNG010000252 DAZSNG010000000
VERSION     DAZSNG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.256"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..546)
                     /gene="ygeQ"
                     /locus_tag="ACHW4Z_004253"
     CDS             complement(<1..546)
                     /gene="ygeQ"
                     /locus_tag="ACHW4Z_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR8883619.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2197)
                     /locus_tag="ACHW4Z_004254"
     CDS             complement(1208..2197)
                     /locus_tag="ACHW4Z_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883620.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2281..>2629)
                     /locus_tag="ACHW4Z_004255"
     CDS             complement(2281..>2629)
                     /locus_tag="ACHW4Z_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883621.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          683 a          613 c          632 g          701 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gagttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa gacaggtgaa
      841 tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac ttgaagcacg
      901 taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt ttggtctggc
      961 tcaggtcagg ctatgggggc ttttggctcg ctctttatat tagatgaggt ctcacatttt
     1021 tcagtcaaaa ctcatcgtct aatagcccat agccagacca ccagtcccag ctttagcagg
     1081 aagaccacga gatagacttt gtgtaggtcc atttggccct cctactttct gtattcacaa
     1141 actagattac tagtacaaaa taaaggccag acatccagtt cggaagtctg accaatagtc
     1201 gttattgtta gttagcttct ttagtgaagc tcaggcgttt gactacaggc aggacagcag
     1261 tagccgagcc agtacccatc ttgatggtcg tagcagcttc agctacaaca aacaccttgc
     1321 cgagaatctc aaggtcgtga gactgggagt ctccatcacg tgaaaggacc acagtaatgc
     1381 tctcgccatc gtgacggaag caaccgaagc tcagagccgt aaaacgctta gccatcatga
     1441 acacgccacg gtcttcaccg ttgtaatagg cgacgtcagt ccccagcata tcgacgatag
     1501 cgtcttcacc accgaagccc tgcttagcag ccagacggga gagagccttg aggtgtgact
     1561 catgggcgat aatgccgtag tcagaaacct cagaaccgaa agaagactcc gtgttctcgt
     1621 acagggcatc catgatagcg tcagccttag cggtcaggct accagtcagg gccggaagtt
     1681 ctacggtctt cagcagcttt gaggactcca gcacgtcaac catcttagag ttaatggtac
     1741 gaatcacgtt atcgcagatg ttcagtgcgt cctgctggag cgtcccctga gtgaaaccag
     1801 tggtcgactt ctggtcccaa agctttgttt ggtgagacac gaggatcgcc tgttcaatcg
     1861 gctcaacggt gatttcagtg ccagacggag cggtcacaac ttcaggtcgc ttaccaccac
     1921 ggacagggac cagcacagag cggtcagcca gagtgtgagc aatgattgga gtcggtaagc
     1981 tgttacgggt cggcaggact tctacaggga acgggatgtt atcttgagcg tctttaagta
     2041 caccgttcag catagcctta acagtcttag ctaactggag ggcatagacc tgagggtcgg
     2101 cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct
     2161 caatgccaaa ctggaaagcg tttttagtca tattcattat aattaatctc cttgagtcaa
     2221 atacttgaag tccctaacag gcagtcaggg accgtgtgtt ctcagatgag ggacgcttgc
     2281 ttacaagctg tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacgtga
     2341 gcgaccagcg gaatgagtag cgctgcggag gttttcacca taggtccggc gttcagcttt
     2401 gacctgttcg cggcgctgta gctctttttg ccattcgtct tcggtcatga acatttcgcg
     2461 ggtacgctct ttctgagacg ctagctcttt agcgatacgc tcagcttcag ctcgtttgtt
     2521 catctggcgg cgttggtagg cggtcatcag gtcattactg aagaagttac caatggtcac
     2581 gccagcttca gccagagctt ccagtaactc catcgcctcg cggctggtg
//
LOCUS       DAZSNG010000253         2585 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZSNG010000253 DAZSNG010000000
VERSION     DAZSNG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.257"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            252..992
                     /gene="phoN2"
                     /locus_tag="ACHW4Z_004256"
     CDS             252..992
                     /gene="phoN2"
                     /locus_tag="ACHW4Z_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR8883622.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            1065..1187
                     /locus_tag="ACHW4Z_004257"
     CDS             1065..1187
                     /locus_tag="ACHW4Z_004257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883623.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1345..2585)
                     /gene="ospC4"
                     /locus_tag="ACHW4Z_004258"
                     /pseudo
     CDS             complement(1345..2585)
                     /gene="ospC4"
                     /locus_tag="ACHW4Z_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          817 a          483 c          456 g          829 t
ORIGIN      
        1 tatgagggaa aatcacaaat catcataata cagagacaaa acgatacgaa aaaatagata
       61 aaaaacatca ttcttttacc acattatttt ccgtgaatat gaaaaataat gttattactt
      121 taatataaga ctattttttg tttttccatc actctgttca aatttttccg catgacttgt
      181 gttttttgta atacagctcg ttttttacag ctgaccaaaa tcatcaatta attatgctaa
      241 ggaaataaat tatgaaaacc aaaaactttc ttcttttttg tattgctaca aatatgattt
      301 ttatcccctc agcaaatgct ctgaaggcag aaggttttct cactcaacaa acttcaccag
      361 acagtttgtc aatacttccg ccgcctccgg cagagaattc agtagtattt caggctgaca
      421 aagctcatta tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg
      481 aagatgcata ctacgagaat tttggtcttg cattttcaga tgcttatggc atggatattt
      541 caagggaaaa taccccaatc ttatatcagt tgttaacaca agtactacag gatagccatg
      601 attacgccgt gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc gttatttata
      661 aagacgcaac ctgtacacct gataaagatg agaaaatggc tatcactggc tcttatccct
      721 ctggtcatgc atcctttggt tgggcagtag cactgatact tgcggagatt aatcctcaac
      781 gtaaagcgga aatacttcga cgtggatatg agtttggaga aagtcgggtc atctgcggtg
      841 cgcattggca aagcgatgta gaggctgggc gtttaatggg agcatcggtt gttgcagtac
      901 ttcataatac acctgaattt accaaaagcc ttagcgaagc caaaaaagag tttgaagaat
      961 taaatactcc taccaatgaa ctgaccccat aaagctggac agcctgtatc aggctatgga
     1021 gggcccatag acaaatctac cctatatgag cataggagga gtctatgggc acaccacgtt
     1081 ttacccctga atttaaggga ttactggaaa ggctgggaca tatccctccg gcagaagcag
     1141 aaaaagctta ttatgctgcc atcggaaacg atgatctggc aacctgagtt cacagataaa
     1201 acattctcta ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt gttgcagggg
     1261 aacggtctgc accatcccct atgtcgatca atataacaga aatgacaact tcagattcag
     1321 ggcacaacct aaatatattt taggtcacct ctcaaatcgt ttgcctgata ccgctccatt
     1381 ttttaataaa aaaatcaatc atttttgaat ctttgctttt cattgcattg tcaagcatag
     1441 tgtccccact atttgctttt tgaaattttt tgtttacatc aaccaatcca ttattgataa
     1501 aatattctag cactttatag ttggcaccat ctttactaag taagtattca acatcataca
     1561 gattacaata tctattgttt ttcattttct ccatctctgc gacatcttgt tttgtaaaat
     1621 taaatttaga tagtaaataa agtgctatat cagctttttt attgcctaaa gaataagcaa
     1681 gggcctgatg agccattttt ttatcagtca ctttattgtt aatctcttca taattagatg
     1741 ctttaatcgc atcctccaaa gatatgtctc tgagttttat ttttttgaag ttatctgtac
     1801 ttagcattct tggtatatga tactcaagct gaaacacaaa gtttataatt ctatccagac
     1861 taacaggatc aatatttttg ttataacaaa actctctgaa tccttgatct ttactctttc
     1921 ttataaaatc aataagatgc aacccaattc caagcctcat atctttagtg ttaaatattg
     1981 gaacatcagt tttcccctga ttaccatgca catacctgct aacttcatcc acaacctctt
     2041 taaagttatc taagaaaaat gattggtgct catggtaaaa aacaggtgga atagcattat
     2101 caaagtgatc ggttaatgtc atatatccat atggagagtc atgatcaatg atatacgcat
     2161 tggcgccaaa atctacagta tgatgtgttt tatttagtgg gagtttttct tgatgattgg
     2221 aaatttcgac cccaaaaaaa acaaaatcgt ggttagagat ttctttaata tcctcagagt
     2281 atgtattctt atgtgtttgt atattttcag actgaatttt atcaaaggat ttaatattaa
     2341 gaataatatt tgacagatta gagttacttt gatgtctgaa gttaaaatta gaattaataa
     2401 taatagaaag taagtgacgt tcgtctgaag aaagaggatg aactttttgt attatctcag
     2461 agatatagtg cctggacacc ttatcgagat gctcaagtga ccgaaatgat gaccgtgctc
     2521 cagatggcgc ttcaagagaa agatttagtg atttgaaact ggtcccttga gaaaacattg
     2581 tgcta
//
LOCUS       DAZSNG010000254         2531 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZSNG010000254 DAZSNG010000000
VERSION     DAZSNG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.258"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            7..657
                     /locus_tag="ACHW4Z_004259"
     CDS             7..657
                     /locus_tag="ACHW4Z_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883624.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHW4Z_004260"
     CDS             629..1063
                     /locus_tag="ACHW4Z_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8883625.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..>2531)
                     /locus_tag="ACHW4Z_004261"
     CDS             complement(1836..>2531)
                     /locus_tag="ACHW4Z_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8883626.1"
                     /translation="NQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFMLS
                     QTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITA
                     IMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICGND
                     FIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKIGH
                     WLSRTVGGPFLIK"
BASE COUNT          763 a          476 c          531 g          761 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt t
//
LOCUS       DAZSNG010000255         2491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZSNG010000255 DAZSNG010000000
VERSION     DAZSNG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.259"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>2491
                     /locus_tag="ACHW4Z_004262"
     CDS             <1..>2491
                     /locus_tag="ACHW4Z_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8883627.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZSNG010000256         2463 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZSNG010000256 DAZSNG010000000
VERSION     DAZSNG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.260"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(746..2350)
                     /locus_tag="ACHW4Z_004263"
     CDS             complement(746..2350)
                     /locus_tag="ACHW4Z_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8883628.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          575 c          526 g          714 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagtagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAZSNG010000257         2447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZSNG010000257 DAZSNG010000000
VERSION     DAZSNG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.261"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..825)
                     /gene="rfbG"
                     /locus_tag="ACHW4Z_004264"
     CDS             complement(<1..825)
                     /gene="rfbG"
                     /locus_tag="ACHW4Z_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR8883629.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLIL"
     gene            complement(818..1708)
                     /locus_tag="ACHW4Z_004265"
     CDS             complement(818..1708)
                     /locus_tag="ACHW4Z_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR8883630.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1738..>2447)
                     /gene="wzx"
                     /locus_tag="ACHW4Z_004266"
     CDS             complement(1738..>2447)
                     /gene="wzx"
                     /locus_tag="ACHW4Z_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR8883631.1"
                     /translation="IFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVS
                     NIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDF
                     KEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNILNVLLIGFFF
                     NALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILD
                     ALLLQWLYTRMCSVYEN"
BASE COUNT          824 a          468 c          324 g          831 t
ORIGIN      
        1 caagatcaaa ttgcctatat aacctttacc caattgatta ttcacaaagc tatcatcagc
       61 taaggcatta gctattttag ttacttcttt tcgataccac ttgtttctca ccaaaccaag
      121 ccgtttgtat tttgctttaa aactaatgtt tgctcccact tggttgtttt catgctgtct
      181 gtacagcatt gttggttttc tatcaatata ccacgaataa tttctcgtcc gcgcaaatga
      241 atataaaaac caatcatgca gacagatatc ttgtgcagca tttttattat ttatgatgaa
      301 cttcttaaat tcaataagcg tttctttttt tataacatat gtacaccccg gacctgctgc
      361 ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta tcaactaaat tttttcgccc
      421 tgatggataa tatgcaatta cattgctgga ataacaattc gcattattaa ataccaagca
      481 gtcaattgca ttttttattt tatctttata ccaaatatcg tcctgatcac taaaacagat
      541 ataatcatag ttttcaagat cgacatcttt aattaatcta taaaaatttt tacctgcccc
      601 cccaaactta ttgacgcttg gaagtatatt tataattgag ggatatgata attgaaattc
      661 ttcacaaata tttaatgtat tatcagttga taaatcatct gatataaaaa ttgagatgtc
      721 tacatctttt tgttcaataa tactttttaa ttgttcctca atccagcatt cgccattata
      781 cgtagccata agtacagcta ctcttttttg atcagaacta tgcattataa cgaccgcccc
      841 cagtaattcc tcttattcca tccagcgtag ccaaaatatg ttttttcata tattcaattt
      901 tatttttttg agttgcgata attaaagtcg tatgaattaa agaaaaaaca acttccctaa
      961 tagagagtaa aagaggcaca tgattttttc taagcaaaag aaatgcattt cttactcgat
     1021 aataacgacg tactggcgag tgaattggta tgttaatccc acagattttt actgacttat
     1081 ccccaatagc atgaaccatt tctatatcag gaatgatatg aactaatatg ccatagttta
     1141 agcatctaag acaccattct gtatcgacat aatcaataaa aagttcctca tccatcattc
     1201 caacaatctc aaaaacttct tttgaaacca tggttccgga tgagattgtt actgaacttt
     1261 taaacggtgt ctgtccttca gtgacatgaa ttttctctcg aagaccattt tttttgatat
     1321 tacaaatagg ataataatgt gacttatctc tatcaataaa aactgggcca gctatttttg
     1381 cattttcatt atccatatag caggaaagtt ttttgataaa ctcattgcga atacggctat
     1441 cttgatcaaa aaaaattatc ttctctgccc ctttttcaag agaatatcga atcccaatat
     1501 tttgtgcttt tgctatacct tcattcctac caagacaaat taaatgaact ttttcaatgt
     1561 ctgctaattt atctttcaag gtgaaattag tcctattatc gactactaca acgtaacaat
     1621 tttgctcctt tagttcagtg atcagtgcat tcagattttt aagttcggga ttatatgtca
     1681 caatgacagc gtaaatatta ctattcatta tccaagtgac tcagtaattg gttaatttta
     1741 gttttcatat acagaacaca ttctagtata aagccattgt aataacagag catctaaaat
     1801 tacacgtatg ctccaggata ttgcggcacc cagtaaccca tatttttcga ccataaaata
     1861 caaaagggct aaataaggaa ccaattcagc acaatgaatc aaagcagtaa ttttagattt
     1921 tcctaatgcc tggattgcag agaatggtat cattgccagc gcattgaaga aaaaaccgat
     1981 caataacaca tttaatatat taaaggaatt aatttgataa ttctcaccaa accatatttt
     2041 caatacaagc cctgaatata acaagcctat gattatcacc ggcaaacaga ttagaaacat
     2101 taaaagcaat gatttattta cattacgttt aaattcttta aagtctttga tgttacttaa
     2161 ccttggaaac actgccctcc cgattgctgc aggtattatt cctaatttta gaataacctc
     2221 cgctggtgca gaataaaatg caactttatc tgcccccata atatttgaca ctataaatcg
     2281 atcaaaatac accatgaccg gacttatgat attacttact gtcatccaac cgccaaagaa
     2341 aaagagtcgt ttaaaggttt tataacaaaa atgaaccccc gaaattttaa tatcattcct
     2401 gacataatag gcagagacta aaatcgaaat aactcttgca aatatta
//
LOCUS       DAZSNG010000258         2371 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZSNG010000258 DAZSNG010000000
VERSION     DAZSNG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.262"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..823)
                     /locus_tag="ACHW4Z_004267"
     CDS             complement(<1..823)
                     /locus_tag="ACHW4Z_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8883632.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="ACHW4Z_004268"
     CDS             complement(1422..1670)
                     /locus_tag="ACHW4Z_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8883633.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1933..2028)
                     /locus_tag="ACHW4Z_004269"
                     /pseudo
     CDS             complement(1933..2028)
                     /locus_tag="ACHW4Z_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2025..>2371)
                     /locus_tag="ACHW4Z_004270"
     CDS             complement(2025..>2371)
                     /locus_tag="ACHW4Z_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8883634.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          437 c          574 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg g
//
LOCUS       DAZSNG010000259         2326 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZSNG010000259 DAZSNG010000000
VERSION     DAZSNG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.263"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..151
                     /locus_tag="ACHW4Z_004271"
     CDS             <1..151
                     /locus_tag="ACHW4Z_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFR8883635.1"
                     /translation="SASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRI
                     QCLGG"
     gene            complement(240..752)
                     /locus_tag="ACHW4Z_004272"
     CDS             complement(240..752)
                     /locus_tag="ACHW4Z_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR8883636.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            947..1711
                     /locus_tag="ACHW4Z_004273"
     CDS             947..1711
                     /locus_tag="ACHW4Z_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883637.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1662..>2326
                     /locus_tag="ACHW4Z_004274"
     CDS             1662..>2326
                     /locus_tag="ACHW4Z_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR8883638.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          658 a          516 c          589 g          563 t
ORIGIN      
        1 cagcgcctcc ggcatgggca atgatgccac cgtcgaactc tctccagttg ctggacgaaa
       61 cgttctcggt atccgggacg gaatcatcag cgaccagaca gcattgagaa tgcttcagga
      121 gtacatcagg atccaatgcc ttggggggta gcggtaattt tactcattat ccttcaaatc
      181 agattctgtt gtcagaggaa tgggggacgc tggattcgca agagcttttt atgggcagtt
      241 tagctaaaaa aaggcaacac gaagctaaaa ctaccaacat aaagtgctga ccagctagca
      301 gctgaaatca gactcactgt atacactttc actggatgaa gttgcaacat tcctgccacc
      361 aaaggagcaa tgtaacgcag cacggcgata aagcgcgaag tgaaaagtat tgacacagag
      421 ttattttgca actgaaggcg aacccgttct attgtattgg aacggctcga tattatacat
      481 gcaagttgag ggatatgccc aattattata ccaagatggt aattaacgat agtaccgcac
      541 catgcgccac tcatgaccgt gattccagct tcccatggtg aaagagtcgt cttgctgact
      601 gtaattacag tggccatcat aattgatgct ggtggcaata tcgccgaaat gagtaaagtg
      661 gattttgtga acgctattat aaaaagaagc ccccataggc gatatggatg aagtacaaaa
      721 tagttcatca aagcatttat ccactccatc aatgtctttc cttctgtgaa tttttctttg
      781 ctatttaacc gtgatacgac ttaatttagg cttaaacacc ttacatttac aaggtactcc
      841 tgtggggggc cctgccacgg agcgtcgcag gagtgagtga tgaggaagaa ttaaagcaac
      901 ctattgtgac aggcagcgat aacagtaaat cacaggaagc ataatcatgg caaaaccgga
      961 ctgggaggcc atcgaatcgg cataccgggc cggagtcctt agcctccgtg atataggcga
     1021 gaaatacggc gttacagaag gggctatcag gaagagggcc aaaaagcttg attgggcacg
     1081 cagtggcggt acgcaggttt gcaaaaatgg tacgcaaaaa aggaaagtgc gtaccagcag
     1141 aaagcctgcc attactggcc ttacacaaaa aagtacgcaa ctaaaaacag aatctacacc
     1201 ggatacgaaa ccgatacgcg gaatgcgtac cgatcccccg actaacccat tccaacccgg
     1261 taaccagcag gcattaaaac acggtggtta tgcccgtcgc cttctgctca aagatgaggt
     1321 gatagaggac gctaaagcgt tgacgctcga ggacgaatta tttcgccttc gtgctaacaa
     1381 ccttgtcgct gcagagaata ttggtcggtg gttggtgtcg ctggaagatg ctaatgggga
     1441 ccaggaaagg aagatgctga ttgaaaatat cagcgccgcc gagaaagcaa tgatgcgcaa
     1501 tacagttcgt attgagtcca tcgttggcac gcttgcgacg gtaggaaaaa tatttgctga
     1561 tacagcctac cgcaaggccg ccactgataa ggtgtctctg gaggctgatc gtcttcgccg
     1621 tgatgcaggt attgatgatg gcaatggaga gcgtgacctc aatgacttct actctgacat
     1681 ccaaaccgac gctgaatccg gctttacgta gtttctggac tatgcgggca cgtaacaaag
     1741 tgctttatgg tggtcggtca tcgtcaaaat catgggatgc cgctggcatt gccatatttc
     1801 tgtcgaataa atacaccctg cgtttttgtt gtgcccgtca gatccagaat aaaatcgaag
     1861 agtcggtgta taccctgctc aaaattcaga tagacaggtt tggtctgcgg caccgtttcc
     1921 gtattctgaa caacaaaatc attaaccggg ttactggctc ggaatttgtt ttttatggat
     1981 tatggcgcaa catcgaagaa attaagtcac tggaggggat cgatatgttg tggttggaag
     2041 aagcccacgc actgacggaa taccagtgga aaattctgga gccaacgatc cgtaaagagg
     2101 gttcggaatg ctggttcata ttcaaccccg gacttgttac tgatttcgtc tggcgcaact
     2161 tcgttgttga tccgcccgaa ggcactctca tccgcaaaat taactatgac gaaaatccgt
     2221 ttctgtctga caccatgctt aaggttatcg acgcggcgcg acgccgtgat ccggatggtt
     2281 ttaaacatgt gtatgagggc gttccggagt ctgatgatga tgcggc
//
LOCUS       DAZSNG010000260         2325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZSNG010000260 DAZSNG010000000
VERSION     DAZSNG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.264"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHW4Z_004275"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHW4Z_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8883639.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHW4Z_004276"
     CDS             282..452
                     /locus_tag="ACHW4Z_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR8883640.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHW4Z_004277"
     CDS             527..802
                     /locus_tag="ACHW4Z_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883641.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHW4Z_004278"
     CDS             902..>2325
                     /locus_tag="ACHW4Z_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8883642.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZSNG010000261         2280 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZSNG010000261 DAZSNG010000000
VERSION     DAZSNG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.265"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..797
                     /locus_tag="ACHW4Z_004279"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHW4Z_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHW4Z_004280"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHW4Z_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHW4Z_004281"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHW4Z_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2123..>2280)
                     /locus_tag="ACHW4Z_004282"
     CDS             complement(2123..>2280)
                     /locus_tag="ACHW4Z_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8883643.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          553 a          554 c          692 g          481 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagcagctt aggggggtgc
     2101 ccctattaag ttgtcaagca tgttatgacc cctgcggggt ataaaaagtc ccgtcgcgca
     2161 tcatggcgaa cagaacgtcg cagcgtcgtc tcgccagggc gataagcgcc tgattgtgtc
     2221 gttttccctg actcattttg cgggtgtagt aagccctgga gagcggatcc ctgagcgcgg
//
LOCUS       DAZSNG010000262         2247 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZSNG010000262 DAZSNG010000000
VERSION     DAZSNG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.266"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1958)
                     /gene="mobF"
                     /locus_tag="ACHW4Z_004283"
     CDS             complement(<1..1958)
                     /gene="mobF"
                     /locus_tag="ACHW4Z_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFR8883644.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPG"
     gene            complement(1958..>2247)
                     /locus_tag="ACHW4Z_004284"
     CDS             complement(1958..>2247)
                     /locus_tag="ACHW4Z_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR8883645.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          413 a          755 c          551 g          528 t
ORIGIN      
        1 cccggtcggt acatatcccg cagataacgg ctccggctgt ccagccagac cggtgacagt
       61 gcagtcatgg tcacctcagg gtgtccgagc acgccctgcg ttttcagctc actgcgaatg
      121 gcctgtgtca gtatggcctg ttcccgtacc ccgctgacct gtgccacgct ctcttctccg
      181 gctttcacgc tggccgcaaa atctccggcc agccgggcat agcggacatt acggtccggt
      241 tcactgatga tggtggccgg tcgctgttct ccgccctgcc agcgatatgt gttcaccccg
      301 gcatccttca tggccatcag cgcactgcct gtaccggttc gctgcccgct gtcggttatc
      361 aggacctgta cgttatgacg tgcggcacca tccagcaggg ttaacgtctc tttcagggag
      421 agtttttcac cctggtcgac gataacggta ctgcccggcg tgaaggccat gccttccagc
      481 agctgacgac gtccggttat cagttcaccg gacagccgtt catcctgctt caggttcatc
      541 tgcgagcgac ggtcagcagc gataatctgc acctcccgcc cctgttcccg ggccatcatg
      601 accagttcag ccacccgctc acgctgcccg gctgcaccgc cctgcccgga cacaatggcc
      661 agcgacggac ggtcctgtgc cagaacactg accgcatcgc tgtaaccagc cgtccggggg
      721 acacttttct ccggatgtac ggtcacccgg ttctgtttca tgatgtcacg actgagtgcc
      781 cggacggaca gctcatcgag cacatgaatt cctgatgtga acagcccctt ctcacggtcg
      841 agggggataa gctgctcacg gctgatggcc tcatcgatac cggcgcgtgc ccgttcaatc
      901 acaccatttt ccggcggcag tatgccgacc gtcctggcca gcacgtccgt atacgtgaac
      961 tgcacttttc gttcacttaa tccggcaatc gcctgtgtca ccgcctgctg cacatccggc
     1021 ccgtcctgtg aagcaggccc gggcgcctgc gtgcggatct ccgcacgctg atccgccgcg
     1081 tcacgatatg cccggatgtc gaacccggtt tccttcagcg tctgcatcca ttcagccatt
     1141 ctgatttccg gatcgacgtg ctgtttggat tttcgcgtat ccagtgccgc cacatcccgg
     1201 gatttcagcg aggcgtcttc accgacggcc tccctgatag cctgtgaacg actggaaaag
     1261 gcctccaccg gtacgcccgg catctcccac ataccgtgct taccgaccac ttcggtttca
     1321 taacccagcg cctcaacgcg ctgacgtaac tcactctgat atattttccc gaacgcaatc
     1381 cggttcgcga gcacgttctc ggaaaatccg gttttcccca ctttgtcact gctcagtgtc
     1441 ttccactcgc cgttatgctg cgtgacatta gccaccacca catgcgtgtg taactgtggg
     1501 tcctgatcgc gactggtgtc atggttaaac agtgccatca ccagattacc ggtcagcacc
     1561 gtttctgact gtccgtccgt catcacccgt gtggaagcca gcgcctccac ctgacggaca
     1621 gcaaaatcca cggcctggtt atgtgcatca ataagacgtt tatccccccc gagcatggcc
     1681 atcatggaga cacttttggg ggcggagaag gtcaggtcgt agccgggacg atgcttatta
     1741 ctgccatcct gcatgcggct taaatccgcc ccgtccggca gcctgccctc cagaagacgg
     1801 gtaaaaacat ccttatcgac gctgccctgc agcccaagct gttcagcccc ctgaccggcc
     1861 cagcgttctc ccatgctgcc cagcacatag taattatcct tgtcggtgta atagtttccg
     1921 gcactgcctg ccgatttaac aacgctgaaa ctcaacatca gaaatcatct cccggctcaa
     1981 catcctcccc gcgctcccgg tgcacattaa tgttcacctc ttcacgacgc tgcatatgct
     2041 gctggatgtc cggatgattt tcctgttgcc atgcctcata agcggccata tccaccactt
     2101 caccggattc actgatcccg ggtggcagtt gctgttccat ctcttcttcc ggtttcattt
     2161 tcagctcctg ctcgatcccc cctgccggaa cactcacacc tgcatctgac ttcttatcat
     2221 tgatgacaga agacactggc tgttgtg
//
LOCUS       DAZSNG010000263         2230 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZSNG010000263 DAZSNG010000000
VERSION     DAZSNG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.267"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..222)
                     /locus_tag="ACHW4Z_004285"
     CDS             complement(<1..222)
                     /locus_tag="ACHW4Z_004285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883646.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACHW4Z_004286"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACHW4Z_004286"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8883647.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZSNG010000264         2224 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZSNG010000264 DAZSNG010000000
VERSION     DAZSNG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.268"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_004287"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR8883648.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHW4Z_004288"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHW4Z_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8883649.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHW4Z_004289"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHW4Z_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHW4Z_004290"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHW4Z_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          539 a          593 c          620 g          472 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 ggta
//
LOCUS       DAZSNG010000265         2219 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZSNG010000265 DAZSNG010000000
VERSION     DAZSNG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.269"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHW4Z_004291"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHW4Z_004291"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR8883650.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHW4Z_004292"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHW4Z_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR8883651.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="ACHW4Z_004293"
     CDS             complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="ACHW4Z_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR8883652.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          589 a          611 c          556 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatggg
//
LOCUS       DAZSNG010000266         2197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZSNG010000266 DAZSNG010000000
VERSION     DAZSNG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.270"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..422)
                     /locus_tag="ACHW4Z_004294"
     CDS             complement(<1..422)
                     /locus_tag="ACHW4Z_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883653.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFN"
     gene            complement(415..1179)
                     /locus_tag="ACHW4Z_004295"
     CDS             complement(415..1179)
                     /locus_tag="ACHW4Z_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8883654.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            1425..2186
                     /locus_tag="ACHW4Z_004296"
                     /pseudo
     CDS             1425..2186
                     /locus_tag="ACHW4Z_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          762 a          426 c          358 g          651 t
ORIGIN      
        1 ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc gagaaaattg
       61 agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc cttagcatta
      121 cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg ggaaaatttc
      181 ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc atcttttttt
      241 aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc atttcttgtt
      301 aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca gagagcaaaa
      361 aaacacacct gtagcaatgt gaaaacaagt attaatataa aatacatttt cttattagtc
      421 atataatagc acctctgaac aaattgcata agtttcgtca ttagaggatt ttatacatct
      481 tgataaatac cgctgccctt ccccaagcaa ataatgcata ctatttacat catatataaa
      541 atttgcacct ggcacacagg gtaaatgctt tttcgaaatc tcagcggcct ctatttgaga
      601 tttagcgatg ccaatctcgt taagattctc tgaaagatat aaatcacagg tgtcaatctt
      661 acctgattta accaatggat aatccgttat actgtaggta tctttaaaag ccacggcaaa
      721 catttcaata ataacaatta ctaccacaag cgaaatcaaa atgcgaaaaa gaggaatact
      781 ccttttgcgc tctacttttt caacaacatc ttcttgtaaa tgagaatcat cataagattg
      841 tttacttatt gttatgtttt cactgatgta aaagcctact ttgggtatcg taagaatcac
      901 attttcttca acaccaatat ttttaaaact tttgcgtaac agggatatat attggttcaa
      961 tgtgttgttt gacagatcaa atgaaaatct ctccaaaacg ctatcaatga ttgagttcct
     1021 gtcaaccgct ccaccattac tttccagtaa cactgccaaa atacaccccg ccgtagctgg
     1081 aaaatttatc tcatcctggg tatttacatt cgagagcgag catttgttcg gatcaaaaat
     1141 aatcgcgtca ttgataatga aaaaacatct ccgctccatg caggcaacaa cacaaaataa
     1201 gattttgtca ttttagccta ttcctggtgg aactgacaca agaaactaaa atacaaatat
     1261 aaccctgaaa aagtcatata aaacttatat ccacagaata aaaaatcaaa catgtcagtt
     1321 ttatcaatat aaagtatcgc aaggcgaaaa gtgatgcaag aatactccta agtaaatgaa
     1381 agcgcaccac atgaaattat caacacatgt aatttcatca ttaggtgcta tcgaaaataa
     1441 ctcaggagct acttaagatg aataatgtct tactcatgag acccgatgac tcagaattta
     1501 tgatacctga tgactggtca gggctaaacg caaaaggcat tacatacgaa caagataata
     1561 gagtgacaga gtcttttctg ccttttaaat ctttttattg ttcaccggtg tgcacgacag
     1621 ttgaactcat ctcataaaat aattgttttg ctatgggtat ccgctacctt ctggaaaaga
     1681 aaagagactt aaggctaaat cactttaaag ccttctctca taaatgcgag acaaaacctg
     1741 acgtgttaat tatgaccggg tgcaccgtcg ctgatactta tagtatattc tcacgcattg
     1801 ttgaagaaaa tataatgaaa acgatgacac ctatcgtcat cattactggg catcactcag
     1861 aagatctttg ttctctggca tcgaaactgg gaggagttaa aataatatca ccgacagaaa
     1921 agattgcaaa aatagaaaac gctatcaatg atgcttttaa cagcaagcct aaaaaaataa
     1981 gatttttaac agatatactc tctcctcaac aaattgagac catgaaaatg gttgcgcagg
     2041 gtagatccgt taatgagatt tcagccgcaa tgaatattag catcaagacc gtgtatgcac
     2101 ataaagttcg tgctatcaat aagctgggaa ttaagaaaaa agtttatgaa gcgctttttt
     2161 ataaagcgct aattcagttc aactgatgta aaaatca
//
LOCUS       DAZSNG010000267         2183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZSNG010000267 DAZSNG010000000
VERSION     DAZSNG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.271"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACHW4Z_004297"
     CDS             <1..203
                     /locus_tag="ACHW4Z_004297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883655.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHW4Z_004298"
     CDS             293..694
                     /locus_tag="ACHW4Z_004298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883656.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHW4Z_004299"
     CDS             696..1301
                     /locus_tag="ACHW4Z_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR8883657.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHW4Z_004300"
     CDS             1288..>2183
                     /locus_tag="ACHW4Z_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8883658.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZSNG010000268         2164 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZSNG010000268 DAZSNG010000000
VERSION     DAZSNG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.272"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            155..340
                     /locus_tag="ACHW4Z_004301"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHW4Z_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..646)
                     /locus_tag="ACHW4Z_004302"
                     /pseudo
     CDS             complement(440..646)
                     /locus_tag="ACHW4Z_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(699..950)
                     /locus_tag="ACHW4Z_004303"
     CDS             complement(699..950)
                     /locus_tag="ACHW4Z_004303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883659.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLRWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            1402..1686
                     /locus_tag="ACHW4Z_004304"
     CDS             1402..1686
                     /locus_tag="ACHW4Z_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR8883660.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1686..1961
                     /locus_tag="ACHW4Z_004305"
     CDS             1686..1961
                     /locus_tag="ACHW4Z_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR8883661.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          600 a          536 c          521 g          507 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagcgcggac aagcgcctta tatccgtcat ccgtctccac accagaaata ctgtatccat
      601 aggtcttcag ccgtgacacg atatcaacct tatccggaat gccctgcgat tcaaaaaata
      661 aatatttagt cagtaatttt atgtttttcc tgaaaaaatt actctttttc gaactccagt
      721 gagcgttcac gactcatcag tgtgaaaatg atggcaaaaa cagcagacag tatcagtacg
      781 ataaatgtgg ccccccatct cagataatcg acaacaagtc ccattcccag tgtcgcaaca
      841 attgagccga agatatagga aaatgtgcct gacagcccta acagaataag tttacgcgta
      901 aatcgccctg aatccagaag agcattaagt atcacataaa caggttccat accagtcctg
      961 accgcatcag gacgccagta aataaaatct cgtagtccac cggcatcaga gacaagagta
     1021 atcgtcgtta ccggtatttc gactggtttg tactggtgtg agcggacaaa actactgtac
     1081 gtctctcata cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
     1141 ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac agaggcattt tcatcgctga
     1201 caggccgcat ggcctcgcag agccggaggg ggactggtga tattcggacg accgtaccgg
     1261 cttgcgccgg gcttcggcgg ttttacacct gtgctacatg taaccaccgt atgccgcctt
     1321 atatgcgctg actcgcatta tttgccttgt tccgtctgaa agtaatcact atgattaaat
     1381 atgattaaca actaatcggg tatgcaaatg aaaaacaata ccgcacaagc aacaaaagta
     1441 attaccgcgc atgtgccatt acctatggct gataaagtcg accagatggc cgccagactg
     1501 gaacgctccc ggggctgggt tatcaaacag gcgctttctg catggcttgc ccaagaggag
     1561 gagcgtaatc gcctgacgct ggaagccctg gacgatgtga catccggaca ggttatcgac
     1621 catcaggctg tacagtcctg ggcggacagc ctcagtactg accatctgtt accggtgcca
     1681 cgctgatgga actgaagtgg accagtaagg cactttctga tttggcgcgg ttatatgatt
     1741 ttctggtgct ggccagtaaa cctgctgccg ccagaacggt gcagtccctg acacaggcgc
     1801 cggtcattct gttaactcat ccacgtatgg gagaacagtt gtttcagttt gaacccaggg
     1861 aggtcagacg gatttttact ggcgagtacg aaatccgtta cgaacttact ggccagacta
     1921 tttatgtatt gcgcctgtgg cacacacgag agaacaggta gattgggtgt ggaggcaact
     1981 ttaacatagc ctgcaggtat taattctgga gcattgttat caggccagat attttgcatt
     2041 ccattaaata atcttgtagg agataaaata aattacgatg aaataacgaa aattaccgct
     2101 cgtgactggc ggcaatatcg cgctccgggc tggcaaataa cccatcagaa acgttattgc
     2161 caga
//
LOCUS       DAZSNG010000269         2135 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZSNG010000269 DAZSNG010000000
VERSION     DAZSNG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.273"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..105)
                     /locus_tag="ACHW4Z_004306"
     CDS             complement(<1..105)
                     /locus_tag="ACHW4Z_004306"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR8883662.1"
                     /translation="MCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGT"
     gene            complement(65..400)
                     /locus_tag="ACHW4Z_004307"
                     /pseudo
     CDS             complement(65..400)
                     /locus_tag="ACHW4Z_004307"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(397..930)
                     /locus_tag="ACHW4Z_004308"
     CDS             complement(397..930)
                     /locus_tag="ACHW4Z_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR8883663.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(996..1625)
                     /locus_tag="ACHW4Z_004309"
     CDS             complement(996..1625)
                     /locus_tag="ACHW4Z_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883664.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1629..1844)
                     /locus_tag="ACHW4Z_004310"
     CDS             complement(1629..1844)
                     /locus_tag="ACHW4Z_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR8883665.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          546 a          568 c          471 g          550 t
ORIGIN      
        1 cgtcccggat accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat
       61 tgcccatgcc ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga
      121 cgagcacccg accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat
      181 cagctaactc cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca
      241 tgtcagtaat ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt
      301 tgtaggtaat ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc
      361 agataaccag agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc
      421 taatgcgctt tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg
      481 gcaatcgcgc ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc
      541 accggcattc agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata
      601 ggggcaaaat gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg
      661 ctccacccat gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt
      721 cgacagcttc attcccggta tgacaggctt accatccacc attgtggcac cacgacagat
      781 ggtccatatg ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag
      841 aaactggtca agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc
      901 cgacaggccg tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa
      961 taacgagcca acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc
     1021 aggctctttc ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca
     1081 tccatgtctg caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg
     1141 caacggccaa tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga
     1201 ttaataattt tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg
     1261 acgaactcac cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc
     1321 ggtgcagacg caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg
     1381 acgtactcag gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt
     1441 tcctgcaaaa acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc
     1501 gcataatgct catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt
     1561 ttattaagct cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc
     1621 ttcattattt actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga
     1681 ttcgtcagat aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac
     1741 tgtgatggcc tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag
     1801 gtgccgtagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
     1861 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca
     1921 aaagcgggcg aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag
     1981 ttaatcggaa taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct
     2041 gcacagcaat tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc
     2101 gggatttccc atcatcgcag actgaaagac tctga
//
LOCUS       DAZSNG010000270         2092 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZSNG010000270 DAZSNG010000000
VERSION     DAZSNG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.274"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHW4Z_004311"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHW4Z_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR8883666.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHW4Z_004312"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHW4Z_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR8883667.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZSNG010000271         2090 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZSNG010000271 DAZSNG010000000
VERSION     DAZSNG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.275"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(177..1037)
                     /locus_tag="ACHW4Z_004313"
     CDS             complement(177..1037)
                     /locus_tag="ACHW4Z_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR8883668.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1220..1651)
                     /locus_tag="ACHW4Z_004314"
                     /pseudo
     CDS             complement(1220..1651)
                     /locus_tag="ACHW4Z_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1717..>2090
                     /locus_tag="ACHW4Z_004315"
     CDS             1717..>2090
                     /locus_tag="ACHW4Z_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8883669.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT          519 a          541 c          480 g          550 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcag gggtctgacg ctcagtggaa cgaaaactca
       61 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      481 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg cagcagtgtt atcactcatg
      661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      781 tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa agtgctcatc
      841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacga
     1021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta ccagggttat
     1081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1201 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     1261 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     1321 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     1381 cttcctgtcg gccctcattc gtgcgttcta ggatcctccg gcgttcagcc tgtgccacag
     1441 ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc ccgtcatcaa
     1501 tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc atatcggcag
     1561 tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc tccaccttca
     1621 tcctcagcaa atccagccct tcccggtctg ttgggcactg ttgcaaatag tcggtggtga
     1681 taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat
     1741 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
     1801 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
     1861 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
     1921 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
     1981 gcgtatctgt accgggccgt cgacagccgg ggccgcactg tcgattttta tctctcctcc
     2041 cgtcgtaaca gcaaagctgc ataccggttt ctgggtaaaa tcctcaacaa
//
LOCUS       DAZSNG010000272         2048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZSNG010000272 DAZSNG010000000
VERSION     DAZSNG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.277"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(59..328)
                     /locus_tag="ACHW4Z_004316"
     CDS             complement(59..328)
                     /locus_tag="ACHW4Z_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8883670.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            385..1053
                     /locus_tag="ACHW4Z_004317"
     CDS             385..1053
                     /locus_tag="ACHW4Z_004317"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8883671.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1369..>2048
                     /locus_tag="ACHW4Z_004318"
     CDS             1369..>2048
                     /locus_tag="ACHW4Z_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8883672.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          381 g          604 t
ORIGIN      
        1 ttaccagcca accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt
       61 acattgtgtc tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt
      121 tcagcaacac ccgatacttc ttccaggctt ccagcaacga gttttcttcc tccgttgcga
      181 tctccagatc tacagcatcc tgaagtggcg caatatgctc actgaatccc tggatgtaga
      241 actgtgtggt gacggtcttc cagccattcg gctcctgctg tatcgaagca taccaggcta
      301 tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat
      361 ttaattcaca aataaaacta taatatggtg aaattaatga aaaaaaacac agatgatggg
      421 gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca
      481 aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat
      541 ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct
      601 gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca
      661 agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga accttatcct
      721 gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct
      781 ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgatgat
      841 ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa
      901 aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa
      961 ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact
     1021 gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgctta
     1081 agacgtgcta cggtttcctt tacgatttgg ggttgcgact ttacctctat tgataatgca
     1141 ttccggcaga acgttcaaat atgaacgtac gatatttaac taaccgaaaa acaaaatata
     1201 gcacaggcga gacatttacc atctaagaaa atagtatcgt ttttttacag ttaaatcaac
     1261 atcacttcct taaaatgaaa acaataaatt tcaatcctga aacattttat ttcagcttat
     1321 tattccccag tatcacattt tatcaggata tccttctgcg aggttattat gcttcctgta
     1381 aataatcccc ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat
     1441 gttcacaata attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa
     1501 aacagggaaa ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg
     1561 gacttatctt gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt
     1621 aatgtaagca ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg
     1681 aatgcaagca gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata
     1741 aatgtgagtg ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt
     1801 aatgtaagtg acaatcacct gtctgtactt ccgaggttac caatgtcatt ggaattactt
     1861 gatgcagcca gaaatgcttt ggaagtaata ccagattttc cagaaagaga tgatcatatt
     1921 ataagaatat tctggcttaa tcagaaccgg atcacggcaa ttccggaaag catacttggc
     1981 ctcagttctg atagcgttgt caatcttaga gaaaatcaac tatctcccag aataatgcaa
     2041 actttgtt
//
LOCUS       DAZSNG010000273         2040 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZSNG010000273 DAZSNG010000000
VERSION     DAZSNG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.278"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..901)
                     /locus_tag="ACHW4Z_004319"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHW4Z_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          475 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZSNG010000274         2036 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZSNG010000274 DAZSNG010000000
VERSION     DAZSNG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.279"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2036
                     /gene="sat"
                     /locus_tag="ACHW4Z_004320"
                     /pseudo
     CDS             1..2036
                     /gene="sat"
                     /locus_tag="ACHW4Z_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          748 a          357 c          400 g          531 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcagat
//
LOCUS       DAZSNG010000275         2029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZSNG010000275 DAZSNG010000000
VERSION     DAZSNG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.280"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1824)
                     /locus_tag="ACHW4Z_004321"
     CDS             complement(<1..1824)
                     /locus_tag="ACHW4Z_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR8883673.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHW4Z_004322"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHW4Z_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883674.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          486 a          579 c          429 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caagtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZSNG010000276         2028 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZSNG010000276 DAZSNG010000000
VERSION     DAZSNG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.281"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            215..452
                     /locus_tag="ACHW4Z_004323"
                     /pseudo
     CDS             215..452
                     /locus_tag="ACHW4Z_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            566..824
                     /locus_tag="ACHW4Z_004324"
                     /pseudo
     CDS             566..824
                     /locus_tag="ACHW4Z_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            873..1451
                     /locus_tag="ACHW4Z_004325"
     CDS             873..1451
                     /locus_tag="ACHW4Z_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883675.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1560..1818)
                     /locus_tag="ACHW4Z_004326"
                     /pseudo
     CDS             complement(1560..1818)
                     /locus_tag="ACHW4Z_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1802..2028
                     /locus_tag="ACHW4Z_004327"
                     /pseudo
     CDS             1802..2028
                     /locus_tag="ACHW4Z_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          649 a          357 c          481 g          541 t
ORIGIN      
        1 gctggctcga atacggtggt gcggctgaga gtcgcagagg ggaagttgcg tccatgttga
       61 ggggaacgga gcaaaaaacc atcagagata cattgaatca atcaatgtaa gcaatattaa
      121 gtgtctatac cgattacttg atgatcaggt tgtgttgaat aaacgcgcat tcttcggtgc
      181 ttttttaatc ttttggggat ttaaattcct atcgatgaac tgtcgatatg aaaaagcgag
      241 catcatactg acgtcaaaca aaggggtcgc agactgggga gaaatgttcg gagatcacgt
      301 gctggcaaca gcgatcctag atcggctgct acacgctgaa tatcaaagga gagagttacc
      361 ggttaaaaga gaaacgtaaa gctggagtgc tgaccaaaaa cacaacgcca atcagtgatg
      421 atgaaatggt ggaaaacgga cagcatcagt aacgaaagta tcttagcagg catgaaaatc
      481 gtccgcatcc cctaaacggt caaaatgctt ctgccgtgaa ggaaggagaa tgccatctca
      541 tcacttatat ctggcgaatg aaacacagat ggtcggcctt gcccccaaac acacggcggt
      601 gcaaaatgga atggcagaaa acttcgtcaa aacgatgaaa tatgattaaa tcagtgttat
      661 gttcgaacct gacgtgttaa cagcagtaaa gtagtctcgc gaaggtgttc aaacattata
      721 atgaatggca tcctcatagt gcgcagggtt atcgcttgct gcggaaatat ctgaagcgac
      781 gtaccagtaa tggggaaaat gatagctggt ttctgaaaat atagaggaaa atgtgatata
      841 tatatcctgt taatatttaa ggagaatata aaatgaaagt ctcgtttaag tctttaggct
      901 atatttttca tgatatatat aataaaaaac acactatcga tgagttcaac gatgtggtaa
      961 gaaaagcagt tttatcaggt aaaattaacg agctgaatgc atgtcataaa gtggcaattt
     1021 ttcttgccga aaaagacaat gagataacaa agaaagacaa agccaagata atagatacat
     1081 taactgaaaa ctattccatt gaatttcaac aattaatgaa tattagtgag cgcacgttaa
     1141 attcatcttt atatattact cctggtgaaa gtggttttgt cagttttgtt aatcgtgaag
     1201 gaaaaatatg tcatactgca tatgtaaaat catcagataa tagcatgaca tattaccatg
     1261 caaatggtag cagcattgac aaatatatta cggatatgtg cggccttatt tgtatgaggc
     1321 atattgagtc tactggcatt attttttata tgcttgatga aaaagtttta tctgccattg
     1381 ctgaatttat gaatgaaaaa ggatggcggg ctgcgttttg ttcagcaaag aacttatata
     1441 aatgtgttta attttagaaa aactatttgg taaaatccaa caagagtcga gaggttcgcg
     1501 ttgcttcagc agggcctcag cctgttcaac cagactcagt tgcaggtcgt gacgcagatt
     1561 tagtggtggt ggttgattgt cagataccag ccgatgatct tgtagtgcat atcatcagac
     1621 tttgagaagc atatggtttt gcgggtaagc cgcttaagat gtgtccgcag gttcagatta
     1681 tgtcgctcta tccgctgagt gcatttcttg cttataacat gactggttga ggacaataaa
     1741 gttcggtata ccggctcagg catcggtcat gtagaaggca atgctaaact ggctcagcaa
     1801 actaagagca gcgcgcatcc acttacccaa tgcggttgag aaaatagcct tcgatcgctt
     1861 tcatgtggcg aaacaaccgg gtgaggtggt ggataaaacc cgccagaatg aacatccgca
     1921 tcttcctgtt gaaagtcgtc gtcaggcaaa gggaactcgc ttcctgtggc agcacagcga
     1981 taaatggatg accgaatcca ggcaggaaaa gctgatatgg ctgcgtgc
//
LOCUS       DAZSNG010000277         1994 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZSNG010000277 DAZSNG010000000
VERSION     DAZSNG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.282"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..114)
                     /locus_tag="ACHW4Z_004328"
     CDS             complement(<1..114)
                     /locus_tag="ACHW4Z_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8883676.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFT"
     gene            complement(170..787)
                     /gene="yeaW"
                     /locus_tag="ACHW4Z_004329"
                     /pseudo
     CDS             complement(170..787)
                     /gene="yeaW"
                     /locus_tag="ACHW4Z_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(1129..1737)
                     /locus_tag="ACHW4Z_004330"
     CDS             complement(1129..1737)
                     /locus_tag="ACHW4Z_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8883677.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            1907..>1994
                     /locus_tag="ACHW4Z_004331"
     CDS             1907..>1994
                     /locus_tag="ACHW4Z_004331"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883678.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT          535 a          445 c          444 g          570 t
ORIGIN      
        1 ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc gtgaagcgtt tcacctgttc
       61 ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt tgatagtctg acattgctgt
      121 actccatcaa aaatcagccc agcggcggaa aataccgccg ccgatgtcat tagtccttaa
      181 acacctgcgc cagcagatta tggaaatggg cgataccatg ttcggaaatg ccactaccgc
      241 tactgtcggc catgatgcgc ccctgaccac gatagccacg cgatttcagc cctttctgta
      301 cgctttcaac cagacgtaaa tcttccggat ggaacacatc gcgataccac tcaatcagcg
      361 atttttgctc gtcggttaac tcttcattgg tgaagtaaat atcgtagttt tgcagggtag
      421 tttcagaatc caccgggaat tcataaatga ccgtcatcat ccctttgatc ggggtgacgt
      481 tcagcatcgt gcacggccac agccagaaac cgtggaatgc cgcatccgta ccctcttcaa
      541 atttaaacga ctgttcggac ggtttggcga aaccgtattg cagcgtccag ttaccgtgca
      601 tggtgtgcca gtaacgatca acctgtacgg agtcggagaa acctagatgc gccggaccac
      661 agtgatagca ctcgagatag ttatcgacaa tgttcttcca gttggcaggc gtgcgggtgg
      721 taaagcgggc cgctagtttc agattgtgga cttccgggca ggcttccagc actttcgcgc
      781 ccagaccggt gtattgattc acttgaagta cgaaaaaaaa ccgggaggac attggattat
      841 tcgggttctg atgggattag atttggtggg gcttgcaagc ctgtagcgca aattttagtc
      901 gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaatacgaa
      961 aaacaaaaac actgcaaatc atttcaataa cagcttcaaa aaacgttcaa aaccgataac
     1021 aaccaggctg taaccaaatg actcatatca caaatcagct catgccgttt aggtatgtta
     1081 catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt catggttgtt atacggcttt
     1141 ttttacatct tgtggttctt gtgaaggagc tacaagacct tcctgttcat aaataccata
     1201 ctttgacaaa atctttttac gagtatctgt tggtaatgat tcaagcattt tggctgtttg
     1261 taaggtttgc tgcgaaatac cttgcaaatc agcctggatg gaatcatcgg aagaaaaaat
     1321 ctcaggcata agagcttcaa atcctttctt tctgaacaac tcaatcgcag aatgcagttc
     1381 cttgtcctct aacaattcga caattgcgat cagtttagtt ttcaatgtgc tgcggtcagg
     1441 ttgagaattt ttattctctt gattattttt atgctcaacc attggcatgc tctgactttg
     1501 gataaaccac tctattggac gccctgttgc gtaggctatt tgtgctaatc gatctaaaga
     1561 cgggtatgtc ttgccagaca ggtagtttct gataacccca tcagaaagtc cgcacttagc
     1621 tgcaaacgcc cggcttgatt ctccattcat tgcctcttca aggcgttcct tgaaacgcat
     1681 tatttccggg ggcataaaaa tgcgctctgc atttttgctt tctttttcac ctgtcatgcc
     1741 caatctctct gttatataaa gaattttaca gaattctttg atttgcactg gttaaaataa
     1801 aaatgcgcat agcttttgaa ttgagcattt ttctgcgcta tcgtttttcc cgatagcggt
     1861 agccggtatc agttaccgaa aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa
     1921 ccaagactgg cacgcagagc agataaaggc tgctgttcgt atgaagggga agacactatc
     1981 tcaattatcc agag
//
LOCUS       DAZSNG010000278         1985 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZSNG010000278 DAZSNG010000000
VERSION     DAZSNG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.283"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(256..330)
                     /locus_tag="ACHW4Z_004332"
     tRNA            complement(256..330)
                     /locus_tag="ACHW4Z_004332"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Gly,seq:tcc)
     gene            complement(334..409)
                     /locus_tag="ACHW4Z_004333"
     tRNA            complement(334..409)
                     /locus_tag="ACHW4Z_004333"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Thr,seq:tgt)
     gene            complement(412..488)
                     /locus_tag="ACHW4Z_004334"
     tRNA            complement(412..488)
                     /locus_tag="ACHW4Z_004334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(452..454),aa:Arg,seq:tct)
     gene            complement(490..565)
                     /locus_tag="ACHW4Z_004335"
     tRNA            complement(490..565)
                     /locus_tag="ACHW4Z_004335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(530..532),aa:Ile,seq:cat)
     gene            complement(742..1431)
                     /locus_tag="ACHW4Z_004336"
     CDS             complement(742..1431)
                     /locus_tag="ACHW4Z_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR8883679.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1428..1787)
                     /locus_tag="ACHW4Z_004337"
     CDS             complement(1428..1787)
                     /locus_tag="ACHW4Z_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8883680.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1787..1975)
                     /locus_tag="ACHW4Z_004338"
     CDS             complement(1787..1975)
                     /locus_tag="ACHW4Z_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883681.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          499 a          542 c          431 g          513 t
ORIGIN      
        1 agtgtgttcc cggggtttgc ttagaatatt ttgtaagttg tccgaagaga tatttacaac
       61 accagaatga tgaatcatca gtcccctgcc agaggttcac tacgtaacct gaccgacaat
      121 gcaccattct ggtgctgaaa aaagagcact ggaactgcaa caaaacaaca tcacagaaca
      181 ggagatggta aggagtcaga cattcccaca caatattgtg tcagtgcaaa taacaacctt
      241 cgtctcaaat ctcgctggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg
      301 ctgaggtaat agccattata cgatgcccgc atatggtgcc gactaccgga atcgaactgg
      361 tgacctactg attacaagtc agttgctctg cctgctgagc taagtcggcg ctggcccacc
      421 accgaggact cgaacctcgg accgtcaact tagaaggttg atgctctatc cggatgagct
      481 agtggtggtt ggtggccctt gctggacttg aaccagcgac ctggcgatta tgagtcgctc
      541 gctctcacca ctgagctaaa gggccggagg cagaataata accatatgtc atcacatctg
      601 caaactcatc tgaccaccag cacgtttaac gtcctgtgcc atttttcagg cataaaaaaa
      661 ccgctcggcg gcgggttttg cttactttac catcgcgtac aaaatcggca aagtatcaga
      721 tttgcatgag atatatgcct tttaatctac ttttgcaata ctttgctttg aaaatgccgc
      781 tttttgttct gaacgtgttc tcatcacaaa caataaagcc tcactatcca gtcggtgaaa
      841 aatgtgtttc attacaaccc agtgaccagt aaatgttttg gaccagtttt tggttgttac
      901 tcccgccagt aatgccagct cctgatattc ataaccttcc ccaccaaaaa gttctgcttt
      961 taccgcctgc gccgccagcc agattaattt tttcaggcgt tcctgtgttt tctctgcaat
     1021 tttcctagtg ccagaatgag ccttaaactc ttcccacgcc cagtgggtta tggtgatctg
     1081 atactcccag caaatacttc cgctgtaaca ccacaacaac caggctttat agtgctcttc
     1141 aagagacaga acagcccgcc gccacgatga tgtcgaaaac gcaacctgac tgaccagagg
     1201 aattgacgcc cccttcgcca gtgattgttt tcccgggatt ggtggattat cccgcgttat
     1261 catttttccg gtcacctcat cgcggtaccg gattttttta cgcctgtaac gccctgtatc
     1321 gaacatggca ttctcctgcc aggcttcaag ctgacctttt gtcgccccac tcaaatcagc
     1381 ggtggcgata atgagctgct cacgaacaaa ctgtaaatac tggttattca tgcacactcc
     1441 agctctgtga tttttatccc caaccgacca ccaggaacag gcagtccgcg cacaatattg
     1501 gtttcatcaa actgctcgtc gtcgataagc aaccccgcat gtgtcagtgc atccagtggt
     1561 gctttcagga tattgtccag gtcacgacgc cgcttatccg gtggctctgc aataatcttt
     1621 atcgccagtc ttccggacag gcttaatttc agtcgctgct gacggacaat aagcgccaca
     1681 tcccggcgat aacgctcacc ggcttttgat acaaaatatg tgctgccacg acgtcgccag
     1741 taagtattca ccgtcggcgg ataaggtaaa ataaactcat ggcgcatcag cgcagcacct
     1801 cctgcaccag cttttcaaac tttccgactc tggtttccag ctctgccaca cagtccacca
     1861 gctcatctac tgctttctgt gcgcgatgct tcgcctgcat cagttcccga agcgcgggta
     1921 ccatattgag gtagcctgag tttaacggac actccttcct gaaatagaat ggcatcagaa
     1981 ggagc
//
LOCUS       DAZSNG010000279         1961 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZSNG010000279 DAZSNG010000000
VERSION     DAZSNG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.284"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHW4Z_004339"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHW4Z_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHW4Z_004340"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHW4Z_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHW4Z_004341"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHW4Z_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR8883682.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1961
                     /locus_tag="ACHW4Z_004342"
     CDS             1708..>1961
                     /locus_tag="ACHW4Z_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8883683.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRP"
BASE COUNT          560 a          428 c          458 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc c
//
LOCUS       DAZSNG010000280         1913 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZSNG010000280 DAZSNG010000000
VERSION     DAZSNG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.285"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(53..1702)
                     /locus_tag="ACHW4Z_004343"
     CDS             complement(53..1702)
                     /locus_tag="ACHW4Z_004343"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8883684.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZSNG010000281         1913 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZSNG010000281 DAZSNG010000000
VERSION     DAZSNG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.286"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(854..1252)
                     /locus_tag="ACHW4Z_004344"
                     /pseudo
     CDS             complement(854..1252)
                     /locus_tag="ACHW4Z_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1255..1621)
                     /locus_tag="ACHW4Z_004345"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="ACHW4Z_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1913)
                     /locus_tag="ACHW4Z_004346"
                     /pseudo
     CDS             complement(1623..1913)
                     /locus_tag="ACHW4Z_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          536 c          459 g          493 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tga
//
LOCUS       DAZSNG010000282         1871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZSNG010000282 DAZSNG010000000
VERSION     DAZSNG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.287"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..355)
                     /locus_tag="ACHW4Z_004347"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHW4Z_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="ACHW4Z_004348"
     CDS             complement(355..1275)
                     /locus_tag="ACHW4Z_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8883685.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1871
                     /gene="pgrR"
                     /locus_tag="ACHW4Z_004349"
     CDS             1413..>1871
                     /gene="pgrR"
                     /locus_tag="ACHW4Z_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8883686.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          467 a          458 c          435 g          511 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtcctg aggtgtactg
     1861 gcaatagcgg a
//
LOCUS       DAZSNG010000283         1833 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZSNG010000283 DAZSNG010000000
VERSION     DAZSNG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.288"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..362)
                     /locus_tag="ACHW4Z_004350"
     CDS             complement(<1..362)
                     /locus_tag="ACHW4Z_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8883687.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHW4Z_004351"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHW4Z_004351"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR8883688.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          549 a          473 c          377 g          434 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atc
//
LOCUS       DAZSNG010000284         1832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZSNG010000284 DAZSNG010000000
VERSION     DAZSNG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.289"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..180)
                     /locus_tag="ACHW4Z_004352"
     CDS             complement(<1..180)
                     /locus_tag="ACHW4Z_004352"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883689.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="ACHW4Z_004353"
     CDS             complement(321..875)
                     /locus_tag="ACHW4Z_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8883690.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="ACHW4Z_004354"
     CDS             complement(872..1162)
                     /locus_tag="ACHW4Z_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR8883691.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="ACHW4Z_004355"
     CDS             complement(1162..1761)
                     /locus_tag="ACHW4Z_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR8883692.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFGAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          469 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgccaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DAZSNG010000285         1827 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZSNG010000285 DAZSNG010000000
VERSION     DAZSNG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.290"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1147
                     /locus_tag="ACHW4Z_004356"
     CDS             <1..1147
                     /locus_tag="ACHW4Z_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8883693.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
     gene            1286..1798
                     /locus_tag="ACHW4Z_004357"
     CDS             1286..1798
                     /locus_tag="ACHW4Z_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883694.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
BASE COUNT          558 a          344 c          456 g          469 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag agcggggata aaggattagt
      901 aaccttaaaa cagaaagagg gtaaacccat ccccttcggc gctgtgatta gttatagcaa
      961 agacactgaa aatatggccg gcattgtagg tgaagatggt attgcttatg tttctggctt
     1021 gagtgcggaa ggcgagttta atgtgaaatg gggttattct aaagatcaat cctgtatcgc
     1081 aaaataccaa cttcctgcga agaaaagtgc atcagggtta taccagatag cggcgacatg
     1141 tttatagttt ctaaattaag aaactcataa tttatttttg tgaagatgtt tccattattt
     1201 ggtggaagca ttttcgcgat agcacgtaaa tgcaaagaaa gaaactgcat tttgcagtgt
     1261 aaatgaaaat catggagaaa atataatgaa gaaaacattg ttagcaatca tgctggcggg
     1321 gacagctttt gcttctcagg cgggaacttt agtaagccag ggtactgaag cgtcagctaa
     1381 tcttactttg accaagccta tcgttgttaa taataccatt caaccagtga aaggggtata
     1441 tagcggtacg ttaactgcat ggacgccttt ggcaacaggt attgttggcg catctgatgg
     1501 gcagagccac gactatgctg taactttccc ggatgatatt tatgcggaga gcagtacttc
     1561 agcggatgca gtaatttctg gtgacaataa cccggaccat aaactgaaag tttccctgac
     1621 aacgcttgag caggatcctc cttcagctgc ctccgaagag attggcggta agcgttatat
     1681 gatgctgaaa aatactggaa caggtggtgc ctatcgcgtc gtttcccata tgaaagaaca
     1741 ggttgttgag ccagactctt acaccatccg tacccaggct tatatctacg cagaataatg
     1801 cagatgatta gttaatttca tggcggg
//
LOCUS       DAZSNG010000286         1817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZSNG010000286 DAZSNG010000000
VERSION     DAZSNG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.291"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="ACHW4Z_004358"
     CDS             complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="ACHW4Z_004358"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8883695.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
     gene            complement(1317..1469)
                     /locus_tag="ACHW4Z_004359"
     CDS             complement(1317..1469)
                     /locus_tag="ACHW4Z_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883696.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1487..1678
                     /locus_tag="ACHW4Z_004360"
     CDS             1487..1678
                     /locus_tag="ACHW4Z_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR8883697.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          568 a          410 c          372 g          467 t
ORIGIN      
        1 attgaatccg cagcatgatg ccatagttct taaccagcat ttccatgcca gggatgcgta
       61 aataacaaag cgcagaccag ggggcatcac gcaacgcgcg attcagggcg cgaacatttg
      121 gcagatgaac caccgggtcc acatacgcaa gccgctgaat gcgtctgact accgctcgct
      181 gacgagttgc cagtacagcc atataattga caataaaaga gaataccaga taactggagg
      241 aggttatggt cagctgcgtg gtatagccag gataaatggg aatgtaattt tgatagctgt
      301 ggatgctgat catcaacacg accgcccaga gcagcgaaat cagcttataa ccatagcgca
      361 ttgctcccca catcatcagg ggcagcaata atgacaaggt ataattggtg ctaaaaattg
      421 tgcttttttc atttaacggc atgcataaca gcaacaataa agcacctaat gccagtagcc
      481 agagcgcgaa ctcttttttg gtgactttgg catcaacctg ctgttttaat tgcgaataat
      541 agctacgtaa ataaaatgga tttcgcacta cccgaatgat gaagtagcac agcgggacac
      601 cgatcagatt acccaccagt aaggcctgat aattgattaa agtcccgagg ttaaaaggca
      661 tgacaccgac cagattttct ctgctcgcca gtaatcctac aaatgcagca aactggaaaa
      721 gtatcagaaa cagcgttgca ggaaacacta tctgccagaa tatacgctgg gaaattaaac
      781 gggtatcacc atgtgaaacg ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac
      841 agagaatgat aatgataata aaattagccg ttaatgagag cgtttcgtaa aaacccagct
      901 ctgcatattt gcggacaaaa atccctaagg tgatccctgg caatgccgcc cagctgaaaa
      961 acatcatcat gctgatcatc agtgccaaag gcagataaaa gagaaaaacc tcaccggaag
     1021 aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca
     1081 gcggaagccc ccaccattta tcacgtattt ttatataagt tgcattcagt ttcatagatg
     1141 ctcagcagaa tcccccacat cctgaaggag gtgtattcag acaggcatcc cacctgactt
     1201 cgaatgatga ttattcatca ctatagagag cattgattct aagtgtcata tgaaagttcc
     1261 aattgatata tatcaaacaa aataaccctg attaatgaat tattacgttt atcatgttaa
     1321 ttcatcatta ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat
     1381 gtatccactg acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta
     1441 atgtaagtac cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac
     1501 tagtatgcga aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag
     1561 ctttatcttc ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca
     1621 gagcaaaaaa gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg
     1681 gttaccttct ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt
     1741 tgggattatc aaagcggcag atattctttc atcttaaatt ttacgtcttt gtcctgactg
     1801 atgtttatcc tgtttgg
//
LOCUS       DAZSNG010000287         1817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZSNG010000287 DAZSNG010000000
VERSION     DAZSNG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.292"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..360
                     /locus_tag="ACHW4Z_004361"
     CDS             <1..360
                     /locus_tag="ACHW4Z_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8883698.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHW4Z_004362"
     CDS             361..726
                     /locus_tag="ACHW4Z_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8883699.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHW4Z_004363"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHW4Z_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHW4Z_004364"
     tRNA            1605..1680
                     /locus_tag="ACHW4Z_004364"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHW4Z_004365"
     tRNA            1682..1758
                     /locus_tag="ACHW4Z_004365"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          501 a          384 c          501 g          431 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcg
//
LOCUS       DAZSNG010000288         1809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZSNG010000288 DAZSNG010000000
VERSION     DAZSNG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.294"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1198
                     /locus_tag="ACHW4Z_004366"
     CDS             173..1198
                     /locus_tag="ACHW4Z_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8883700.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHW4Z_004367"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHW4Z_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZSNG010000289         1789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZSNG010000289 DAZSNG010000000
VERSION     DAZSNG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.295"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            311..505
                     /locus_tag="ACHW4Z_004368"
     CDS             311..505
                     /locus_tag="ACHW4Z_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883701.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHW4Z_004369"
     CDS             866..994
                     /locus_tag="ACHW4Z_004369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883702.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHW4Z_004370"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHW4Z_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          543 a          393 c          377 g          476 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataat
//
LOCUS       DAZSNG010000290         1770 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZSNG010000290 DAZSNG010000000
VERSION     DAZSNG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.296"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..720)
                     /gene="spa29"
                     /locus_tag="ACHW4Z_004371"
                     /gene_synonym="spaR"
     CDS             complement(<1..720)
                     /gene="spa29"
                     /locus_tag="ACHW4Z_004371"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR8883703.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKV"
     gene            complement(720..980)
                     /gene="spaQ"
                     /locus_tag="ACHW4Z_004372"
     CDS             complement(720..980)
                     /gene="spaQ"
                     /locus_tag="ACHW4Z_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR8883704.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(995..1645)
                     /gene="spa24"
                     /locus_tag="ACHW4Z_004373"
                     /gene_synonym="spaP"
     CDS             complement(995..1645)
                     /gene="spa24"
                     /locus_tag="ACHW4Z_004373"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR8883705.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            complement(1650..>1770)
                     /locus_tag="ACHW4Z_004374"
     CDS             complement(1650..>1770)
                     /locus_tag="ACHW4Z_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFR8883706.1"
                     /translation="DDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISSWMVKE"
BASE COUNT          720 a          334 c          270 g          446 t
ORIGIN      
        1 aactttagaa aagtaaatag tagaagaaca gatgaaaata ataaatattg caagtaaact
       61 tttaatagtt aatgatacgg aaaaagcatt catctgcgga gcaaatctcg ataatacacc
      121 aagtaatact tctgatagta acaatactat cataacagga ctggctaaaa taacagcctg
      181 acttgccaat aaagtcagaa atgttaagat atttgagaca cggaaagaac attgagaaaa
      241 taacgggcat atattataag acaattgtat ggattctaaa ataaagacca taccaccact
      301 gtatagaaat acaactgcag aaaaaagatt gaaaaatttt gccaactcag acgtatcaac
      361 accattggca ggatcaattg aactactaag cgttgcccca cgctggttgt caataatgct
      421 accaacagca tgaaatatcc aaaagggaag agacaagcaa aaagagagaa ggaggccaac
      481 aattatttcc ttgaacataa ggaaataaac atgttcaaaa acagaagaac ctatgtctac
      541 cttaccagaa gtaattaatc ctgatgcaac aagaaaaatc acaggtattc taatagatgg
      601 agaaattata ccgttattta aaaatggaag aaagaaaaat aatggagcca atctaaaaaa
      661 aacgccgttc aggagtatta aaaacacatg aatactttcg aaccagcttg aaatgtccat
      721 caaaccccac tcttaattaa aaacattatt tcatgacaaa aagacaataa aacctcacca
      781 taccatccag aaagaagtag caaagatatt gagacaccta taagctttat accaaaagga
      841 agtgtctgct cttggagttg agtcactgtc tgtaacaaac caatagataa tccaataacc
      901 gtagctatac ctactggcca taaggaaaag ataaggatta aataaagagc cttattaccc
      961 atataaacta tatcagacac attacctcca tcatctaagc aggaatattg atatattgtt
     1021 caatcaaggc cttagacagg ataccccacc catcaagcgc aacaaataat acaagtttta
     1081 ttggtactga tattgtgatt ggactcatca tcatcatacc aagtgcaagt aaaatgcttg
     1141 aaataacaag atcaacaaca acaaatggca aatataaata aaaacctatt ttgaacgcat
     1201 ctttgatttc acttagcgca tatgcaggca agagggaaaa caatgaataa tcattattct
     1261 ctgcactctt taaatcagca ttttcttctt cagacctttg gaaaaaacgt gctaactcaa
     1321 gatctgtatg ctttttcaag tattgcttat attccatcaa gccagaatca gaaaaacgga
     1381 caatatcaga tattgtatca aatttttgag ggccatttag ataattttca taaccagctt
     1441 caataatggg tttcatcacg aacaaagcca taatcaatgc aataccattg agagtcatat
     1501 ttgatggaac ctgctgcaaa ccaagtgcat ttcgtaccat tacaaaaaca atagaaaact
     1561 ttatataaca ggtaccagca gcaactaaaa atggtaatag cgtaaaaaaa gaaagtgtag
     1621 cgatgaggga catgtcactc agcatgagat tactccttta ccatccaaga actaatctcg
     1681 ataccataac catcctcaat agaaacaagt tccccatgcc caaccaaaga accatttact
     1741 ttaatattta catgtttaac tatgtcatcg
//
LOCUS       DAZSNG010000291         1759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZSNG010000291 DAZSNG010000000
VERSION     DAZSNG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.297"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..263)
                     /locus_tag="ACHW4Z_004375"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="ACHW4Z_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHW4Z_004376"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHW4Z_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR8883707.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1163..1348)
                     /locus_tag="ACHW4Z_004377"
     CDS             complement(1163..1348)
                     /locus_tag="ACHW4Z_004377"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8883708.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1373..1645)
                     /locus_tag="ACHW4Z_004378"
     CDS             complement(1373..1645)
                     /locus_tag="ACHW4Z_004378"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR8883709.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAZSNG010000292         1748 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZSNG010000292 DAZSNG010000000
VERSION     DAZSNG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.298"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            454..1302
                     /locus_tag="ACHW4Z_004379"
     CDS             454..1302
                     /locus_tag="ACHW4Z_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8883710.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="ACHW4Z_004380"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="ACHW4Z_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1636..>1748
                     /locus_tag="ACHW4Z_004381"
     CDS             1636..>1748
                     /locus_tag="ACHW4Z_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883711.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          500 a          398 c          411 g          439 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagca
//
LOCUS       DAZSNG010000293         1744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZSNG010000293 DAZSNG010000000
VERSION     DAZSNG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.299"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..353)
                     /locus_tag="ACHW4Z_004382"
     CDS             complement(<1..353)
                     /locus_tag="ACHW4Z_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR8883712.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHW4Z_004383"
     CDS             complement(350..940)
                     /locus_tag="ACHW4Z_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8883713.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHW4Z_004384"
     CDS             complement(927..1298)
                     /locus_tag="ACHW4Z_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8883714.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHW4Z_004385"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHW4Z_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8883715.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          392 a          527 c          408 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZSNG010000294         1742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZSNG010000294 DAZSNG010000000
VERSION     DAZSNG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.300"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>1742)
                     /locus_tag="ACHW4Z_004386"
     CDS             complement(<1..>1742)
                     /locus_tag="ACHW4Z_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR8883716.1"
                     /translation="PDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQL
                     QAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPS
                     QVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGR
                     TPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRL
                     STWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPD
                     TIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQH
                     IDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQ
                     GWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGG
                     DSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSG
                     DAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVW
                     GDIPDNSVQPGAGNG"
BASE COUNT          308 a          603 c          462 g          369 t
ORIGIN      
        1 ccatttccgg ctcccggctg gacgctgttg tccgggatat ccccccacac gcggccaccg
       61 gtgatggcac cgggattcca cggacggcct tcaccggcga ttcttaccac cacaccactg
      121 tcaggattat cacgggcaat ccggacgcca tcctgcatat tatcagccag caggctttct
      181 ccgttacggc tgccctgcag ggccacgaac tgcgcatccc cgctgccttt tacccgacct
      241 tcaccgctga atccccgcag cccgtttccg tcatccgtgg tcagcgggtt cagccagata
      301 ccggcggact tgccgttacg gtcaaacgcc ggcagtgcca catacggttg cggatattta
      361 cgtcccggag caataaaccg tgcaggactg tctcccccgg ccagccccgc ctgacggaga
      421 acggcacggc ctgccgccac gtcccgcagc tcccgcgccg tactgaacag ccgctctgca
      481 ttcatgacct cccggtccgg tttcggctca aacacatcgt gggcagttcc tttctgtacg
      541 gcattgttga tggcatccgt ccagccctga cggttatcgg tgtacacctg cacatgctgc
      601 ttcatacgcg acagggccac gtaggctgac tcaaagccgg ccatcagttt ccggttacct
      661 tccgtgcctt caagcgcgat ggcaaaggtt tcactcgccc cctgtgcacc gtgggcggtg
      721 atggcatagg ccaggtcaat atgttgctct gcccgctcct gaccggggcg aatcacacgg
      781 gtctgctgac cgtcagaaag tgtgacactg tcaccggaaa ccgctgtcac cgtccagacg
      841 ctgttggcca cataaccgcg ctcccggtca ctcttcgtga agcgtatcct gtcaccggtc
      901 cccacccgga tggtgtccgg ggtgtacagc gtgacgcctt cagccaccgc ctcccggggc
      961 gaaatcagcc gcgtgttacc ttccgcatcc tgcagggtta tcagcccgtc atccttactg
     1021 ataccggcaa tccggtgata cacattatcc accagggcaa gggcatccgg gttattctcc
     1081 caggtggaga gacgacgcag ctccccgtcg cgtatattcg ctgtgttcag gacaggcacc
     1141 atgacctgca ccttccccag ttctccggcc ttttcccgtg catcatgaat catgctgttc
     1201 agtacgcgcc ggtcctcatt caggtgcgtg acaatcagcg tctgctcccg tgcttccggt
     1261 gtcctgccgg tatagtcgcg gacaatggct tcatacagtg tcatggggac atccggaaaa
     1321 gcctcgcctt tcagcatcgc cttctgctgc gcttctgcca gtttcgcttc ctggctgtga
     1381 ctgaactccg tcacggagtg ctccggtgcc catgcgccct ccagacgtgg cacctgagac
     1441 ggtttcacac gctcaagccc ggacagcgcc ctttccacat cccggttaat caggctgtat
     1501 accgcctccc gcagttccgg cgtctgacgc acaatctcct tcatgatgac cacatcggca
     1561 gcactgcgcg tctgctggag acggaaaggc tgaccgggcg cgatggcctg cagctggtcc
     1621 gtgtcaccgc tggccacagc ccgaccgcca ccggccgcaa tcagggcgta tgcccgtgcc
     1681 atgtcggtat tgcccaccat agagctctca tcgagaagga acagcgtgtt gctgaaatcc
     1741 gg
//
LOCUS       DAZSNG010000295         1735 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZSNG010000295 DAZSNG010000000
VERSION     DAZSNG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.301"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            71..166
                     /locus_tag="ACHW4Z_004387"
     CDS             71..166
                     /locus_tag="ACHW4Z_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR8883717.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHW4Z_004388"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHW4Z_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR8883718.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          642 a          288 c          278 g          527 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc gtcgt
//
LOCUS       DAZSNG010000296         1718 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZSNG010000296 DAZSNG010000000
VERSION     DAZSNG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.303"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(110..577)
                     /locus_tag="ACHW4Z_004389"
     CDS             complement(110..577)
                     /locus_tag="ACHW4Z_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR8883719.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHW4Z_004390"
     CDS             complement(643..882)
                     /locus_tag="ACHW4Z_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR8883720.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACHW4Z_004391"
     CDS             complement(1017..1622)
                     /locus_tag="ACHW4Z_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8883721.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          550 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaa
//
LOCUS       DAZSNG010000297         1696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZSNG010000297 DAZSNG010000000
VERSION     DAZSNG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.304"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..77)
                     /locus_tag="ACHW4Z_004392"
     CDS             complement(<1..77)
                     /locus_tag="ACHW4Z_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR8883722.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFL"
     gene            complement(757..831)
                     /locus_tag="ACHW4Z_004393"
     tRNA            complement(757..831)
                     /locus_tag="ACHW4Z_004393"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(796..798),aa:Gly,seq:tcc)
     gene            complement(835..909)
                     /locus_tag="ACHW4Z_004394"
     tRNA            complement(835..909)
                     /locus_tag="ACHW4Z_004394"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(874..876),aa:Thr,seq:tgt)
     gene            complement(912..988)
                     /locus_tag="ACHW4Z_004395"
     tRNA            complement(912..988)
                     /locus_tag="ACHW4Z_004395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Arg,seq:tct)
     gene            complement(990..1066)
                     /locus_tag="ACHW4Z_004396"
     tRNA            complement(990..1066)
                     /locus_tag="ACHW4Z_004396"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1030..1032),aa:Ile,seq:cat)
     gene            complement(1256..>1696)
                     /locus_tag="ACHW4Z_004397"
     CDS             complement(1256..>1696)
                     /locus_tag="ACHW4Z_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8883723.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          464 a          446 c          347 g          439 t
ORIGIN      
        1 tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc tgttgatatt
       61 ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga aagaaaaaag
      121 gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg aaaaaacacg
      181 cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc attcttaatt
      241 catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc ccaatgtttt
      301 tgctggcggg tccttttttc tacggtcctc ctccccggag gggcatttca ttatttttct
      361 aatcgttact ggtgagaagg cggaagaaaa agccagttct tcgcccctaa ataacagcat
      421 gattccagtc catgcaaaat ctgatttcca gctcacacat accgagcact gtacaaaaaa
      481 ccgccagcca aggcagttaa gagtgtattc ccggggtttg cttagaatat tttgtaagtt
      541 gtccgaagag atatttacaa caccagaatg atgcattatc agtcccctgc cagaggttca
      601 ctacgtaacc tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac tggaactgca
      661 acaaaacaac atcacagaac aggagatggt aaggagtcag acattcccac acaatattgt
      721 gtcagtgcaa ataacaacct tcgtctcaaa tctcgctgga gcgggcagcg ggaatcgaac
      781 ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg catatggtgc
      841 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
      901 taagtcggcg ctggcccacc accgaggact cgaacctcgc accgtcaact tagaaggttg
      961 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
     1021 ctggcgatta tgagtcgctc gctctgacca actgagctaa agggccggag gcagaataat
     1081 aaccatatgt catcacatct gcaaactcat ctgatcacca gcacgtttaa cgtcctgtgc
     1141 cgtttttcag gtataaaaaa aaccgcataa agcgggctct ttcaaatgtc catgtctgct
     1201 attcgcctcg cggtacagct ttgcgaagcg tacaagaatt gaagcagttt ttacgtcaaa
     1261 aagcaataac ttttttctct ataccaaaag ccataaccat tggtttgtac aaaataaatt
     1321 ctgccacctt tagccaatgc tcaatgcgtc tttcacaggt tcttaaactc catttcgggt
     1381 gtgcatcatt cagcagttca gccattttgc atttggtcat cccccgcccc tcataccgtt
     1441 gccgaaggac gctaatcaat cctggatgct ctgccagcac ctcacttatg acccgatcaa
     1501 tacataacgc ctctgcatca gtacaatgcg ccagccagct cttttgctta ccgttaatca
     1561 tatcccgcaa aaaagcctca agttcagact tgttcagacc tgcttttttc atcctccgga
     1621 gcgcctcgtt aattgccgtt tttgtcagct ttttagaggt caacatcagg ttaaacatat
     1681 tccccgtctt accgcc
//
LOCUS       DAZSNG010000298         1693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZSNG010000298 DAZSNG010000000
VERSION     DAZSNG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.305"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..176
                     /locus_tag="ACHW4Z_004398"
     CDS             <1..176
                     /locus_tag="ACHW4Z_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883724.1"
                     /translation="KKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRFLIFNTEQ
                     SGFYEINDVYVRK"
     gene            410..538
                     /locus_tag="ACHW4Z_004399"
                     /pseudo
     CDS             410..538
                     /locus_tag="ACHW4Z_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            728..1132
                     /locus_tag="ACHW4Z_004400"
     CDS             728..1132
                     /locus_tag="ACHW4Z_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883725.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1195..1693
                     /locus_tag="ACHW4Z_004401"
                     /pseudo
     CDS             1195..1693
                     /locus_tag="ACHW4Z_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          487 a          386 c          429 g          391 t
ORIGIN      
        1 taaaaaaaga atatcagatg ctttgttata ataatagttg gtattatgtt cttgctcgtt
       61 atgattcacg gctcgtttta tctagttcat ttaaagatga cagtaacaga ttcttaatat
      121 ttaatacaga acagtcggga ttttatgaaa tcaatgatgt ttatgtcaga aagtaataat
      181 cctctcccac aaagtgaatt aaatgaatat ataatgcgtt cgaagtatat gtattgagct
      241 gtcatactga gaaacaaacc ttagggcagc acaattaaag ccagaaacaa ctatgcaaga
      301 aagttactaa ataagtgctc ggaacagtaa aacactcagg ggggactcag tccggattat
      361 gtaatcagat aaattgccaa acatcacaaa tcaccagccg gactgagtga tgccgagcat
      421 tgcaccaata ccccgtgatg aacgatgcct gatgcagaaa gctccctata aaacaaagaa
      481 caaaaactac gaccggcgat tcaccgtcat actgatgctg catcgtggtg acactgtctg
      541 tcactttcat gttgactgaa caactctgct gcgaagaagg tgtaccgccg gcgggatttg
      601 attgtccggc gtctagcttc cacttccctg atggaactga aagttgttat ggccggtcag
      661 cttgccctgt ccgcaggaca tgaggtgctg tgacggacag caccagtaac ggaaagtgac
      721 aatgaaaatg aatactgcgc tgatagttgc actaatgtgt atgtggtatg cggtacccgc
      781 tgctgcaaaa gagactttac tggctatgcc acgtaattca acagaacatt gctatgcgga
      841 gattaatgtg cacggaccat atggggtgta tttccgagtg gtcccgcatc cccccggggg
      901 aaagagctgg gtcgaatgta acagtgacta ctattactct gataaaccac cgggagtaca
      961 gatactcgga acccgtgcag ggtgtcgggt atacggaatc tgcgggacca cctcgacact
     1021 gcatgtggct ggaagagggg ttgtgtgtat taaaaatata tgcagtccac gtgggatgat
     1081 aattcacagg atacggaaac gtcctgtagt ggctgtaagc gatgagatgt gatcttaccc
     1141 agcaatagta gacacgcggc taagtgagta aactctcagt cagaggtgac tcacatgaca
     1201 aaaacagtat caaccagcaa aaaaacacgt aaacagcatt cgcctgaatt ttgcagtgaa
     1261 gccctgaagc ttgctgaacg catcggtgtt gctgccgcag cccgtgaact cagcctgtat
     1321 gaatctcagc tctatgcgtg gcgcagcaaa ctgcagcaac agatgacctc gtctgagcgt
     1381 gaaagtgaac tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
     1441 tccaaaaggc cgcgacatac tttgcgcagc ggctggtata cgtggtgtca gcgtcggaca
     1501 ggaataagcc ctcgtcagca gttccgtcag cactgcgaca gcgttgtcct cgcggcggcc
     1561 tttacccggt caaaacagcg ttacggtgca ccacgcctga cggatgaact gcgtgctcag
     1621 ggttaccact ttaacgtaaa aaccgtggcg gcaagcctgc gccgtcaggg gctgagggca
     1681 aaggcctccc gga
//
LOCUS       DAZSNG010000299         1692 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZSNG010000299 DAZSNG010000000
VERSION     DAZSNG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.306"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..642
                     /gene="spa40"
                     /locus_tag="ACHW4Z_004402"
                     /gene_synonym="spaS"
     CDS             <1..642
                     /gene="spa40"
                     /locus_tag="ACHW4Z_004402"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR8883726.1"
                     /translation="TIKEFFKSILLLIILALTTYFFWINDRKIIFSQVFSSVDGLYLI
                     WGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETKSR
                     RRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAV
                     RKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH"
     gene            664..897
                     /locus_tag="ACHW4Z_004403"
     CDS             664..897
                     /locus_tag="ACHW4Z_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFR8883727.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(1504..1692)
                     /locus_tag="ACHW4Z_004404"
                     /pseudo
     CDS             complement(1504..1692)
                     /locus_tag="ACHW4Z_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          533 a          263 c          327 g          569 t
ORIGIN      
        1 acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt aacaacctat
       61 ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag tgttgatggc
      121 ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt ggcattttct
      181 attcttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa agatatgatg
      241 atggataaac aggagataaa aagagaatat atagagcaag agggacactt tgagacaaag
      301 tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc tgatatacgt
      361 aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta ttttaatcca
      421 gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc cttggctgtc
      481 agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt agctagaaaa
      541 ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga tgaagtccta
      601 cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa ggagatgtgt
      661 tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt tgaaatagaa
      721 tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt ccttagcaat
      781 aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct ggttagaaaa
      841 gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga tttataatca
      901 ttcataataa ctcttttgct gatataaata tttttgttga agatatttat gtagtcacag
      961 gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa tatatgataa
     1021 gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag agagactatt
     1081 catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag tctgtaaatg
     1141 atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca gatagtaacg
     1201 gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa aggagaatag
     1261 cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa ctatattgac
     1321 cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt tgatatgttt
     1381 actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc cttgcggctg
     1441 aactggctaa aggcctcaaa actgaggccg acctcaatca gttttcccgt atgctgacga
     1501 aatatcccgg ctcttaaaac gatagtggct caggctttca tttttcagcg ttccccagaa
     1561 gatttccatc ggagcgttgt cgtaacagtt acctttacgc gacattgatg ttttcagacc
     1621 agactgctcc tgtatgaccc ggtaatcgta tgcgcagtac tgtgaacctc gagcggagtg
     1681 gtggattagc cc
//
LOCUS       DAZSNG010000300         1687 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZSNG010000300 DAZSNG010000000
VERSION     DAZSNG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.307"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHW4Z_004405"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHW4Z_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR8883728.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACHW4Z_004406"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACHW4Z_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHW4Z_004407"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHW4Z_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR8883729.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHW4Z_004408"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHW4Z_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR8883730.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          461 a          349 c          384 g          493 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaacc
//
LOCUS       DAZSNG010000301         1603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZSNG010000301 DAZSNG010000000
VERSION     DAZSNG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.308"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(335..481)
                     /locus_tag="ACHW4Z_004409"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHW4Z_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHW4Z_004410"
     CDS             535..717
                     /locus_tag="ACHW4Z_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR8883731.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHW4Z_004411"
     CDS             complement(818..1147)
                     /locus_tag="ACHW4Z_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR8883732.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACHW4Z_004412"
     CDS             complement(1319..>1603)
                     /locus_tag="ACHW4Z_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883733.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          437 a          368 c          376 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctaccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAZSNG010000302         1596 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZSNG010000302 DAZSNG010000000
VERSION     DAZSNG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.309"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            29..1231
                     /gene="virA"
                     /locus_tag="ACHW4Z_004413"
     CDS             29..1231
                     /gene="virA"
                     /locus_tag="ACHW4Z_004413"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8883734.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            1410..>1596
                     /locus_tag="ACHW4Z_004414"
     CDS             1410..>1596
                     /locus_tag="ACHW4Z_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8883735.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          566 a          329 c          272 g          429 t
ORIGIN      
        1 aataggaaaa tacatcagga gaaatcaaat gcagacatca aacataacta accatgaaag
       61 aaatgactca tcatggatgt caactgtcaa atcaactaca gaagtaagtt ggaataaatt
      121 gagtttttgt gacattttac tgaaaataat aactttcggc atatattctc cacacgaaac
      181 tctagccgaa aaacatagcg aaaaaaaact aatggatagt ttctcaccat cactatcaca
      241 agataaaatg gacggagagt ttgcccacgc aaacatagat ggcatttcaa tacggctatg
      301 ccttaacaaa ggcatatgtt cggtttttta tcttgatgga gataaaatac aatccactca
      361 actttcatca aaagaatata ataacttgtt aagttcactc ccccccaaac aattcaactt
      421 aggaaaagta catactatta cagctccggt atcagggaat tttaaaactc acaagcctgc
      481 accagaagtt attgaaacag ccattaattg ctgtacatcg ataataccaa acgatgatta
      541 cttccacgtt aaagacactg attttaatag tgtttggcat gatatttaca gagatattcg
      601 agcatctgac agcaatagta caaaaatata tttcaacaat attgaaatac cactaaaatt
      661 aattgcagat ctaattaatg agttaggaat taatgaattc attgatagca aaaaagagct
      721 tcaaatgcta agttacaacc aagtaaataa aataatcaat tcaaacttcc cccaacaaga
      781 cctatgtttt caaacagaaa aacttttgtt tacttcactc tttcaagacc ctgcatttat
      841 atctgcacta acatctgcat tctggcaatc tcttcacatc acgtcttcct ctgtggaaca
      901 catatatgcc caaattatgt ccgaaaacat agaaaacagg cttaatttca tgcctgaaca
      961 acgagttatt aacaattgtg gacatattat aaaaatcaac gcagttgtcc ctaaaaacga
     1021 cactgcaatc tctgcctctg gggggagggc ttacgaagtt agctcatcaa tattaccatc
     1081 gcacataaca tgtaatggtg ttggtattaa taaaatagaa acaagctatc tcgttcatgc
     1141 tggaacactt ccaagcagtg aagggcttcg caatgctatc cctccagaaa gtcgtcaagt
     1201 atcatttgcc atcatatctc ctgatgttta agccaatatt atattggctg ccagactgta
     1261 aaaaaacgtt cttcagaaca gctctataaa ctattaaacc aaagctaaaa ggatggggac
     1321 caatagaatc tcagcccatc ctttttatat cattatgaac cgccccggaa atcctggaga
     1381 ctaaactccc tgagaaagag gtaaacagga tgactaaaaa tacacgtttt tcccctgaag
     1441 tccgtcaacg ggcagttcgt atggttctgg aaagtcaggg cgaatatgac tcacaatggg
     1501 cagcgatttg ttccattgcc ccaaagattg gctgtatact ggaaactctg cgtgtctggg
     1561 tacgccagca tgagcgggat accgggggcg gtgagg
//
LOCUS       DAZSNG010000303         1581 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZSNG010000303 DAZSNG010000000
VERSION     DAZSNG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.310"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHW4Z_004415"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHW4Z_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR8883736.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHW4Z_004416"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHW4Z_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR8883737.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZSNG010000304         1576 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZSNG010000304 DAZSNG010000000
VERSION     DAZSNG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.311"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(135..>1576)
                     /gene="ospC2"
                     /locus_tag="ACHW4Z_004417"
     CDS             complement(135..>1576)
                     /gene="ospC2"
                     /locus_tag="ACHW4Z_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR8883738.1"
                     /translation="AVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSSSGISE
                     KHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDK
                     VSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSE
                     NIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDM
                     RLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFK
                     KIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSKFNFTK
                     QDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKANSGDTM
                     LDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          487 a          280 c          250 g          559 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaattg tttttgaatc tttgcttttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tactaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg atatcttgtt ttgtaaaatt aaatttagat
      421 agtaaataaa gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacaggatca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tccacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgctt
//
LOCUS       DAZSNG010000305         1556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZSNG010000305 DAZSNG010000000
VERSION     DAZSNG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.312"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            624..1223
                     /locus_tag="ACHW4Z_004418"
     CDS             624..1223
                     /locus_tag="ACHW4Z_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8883739.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          373 c          353 g          422 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggct
//
LOCUS       DAZSNG010000306         1478 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZSNG010000306 DAZSNG010000000
VERSION     DAZSNG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.313"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            98..358
                     /locus_tag="ACHW4Z_004419"
     CDS             98..358
                     /locus_tag="ACHW4Z_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR8883740.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            364..1461
                     /locus_tag="ACHW4Z_004420"
     CDS             364..1461
                     /locus_tag="ACHW4Z_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8883741.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          453 a          336 c          360 g          329 t
ORIGIN      
        1 attagctcct tctgatgcca ttctatttca ggaaggagtg tccgttaaac tcaggctacc
       61 tcagagcgct tatccgattt ttgatcgtat tgaagaaatg gcatgggctc gtcactacca
      121 gcagatcgtt cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc
      181 tcagcacctg tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca
      241 ggcaatcagc cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat
      301 ccggtacatg gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa
      361 tctatggcgc tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg
      421 gggtttgctg gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg
      481 cttatgcggc aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa
      541 acaggctctg actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca
      601 aaaacccgct cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc
      661 gtaatgatca tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga
      721 cgccgaaacc gtaaacgagg actggaattt tcagactggg cgtggctcaa acaggagtgg
      781 cgacgcttta ccgatcgatt tgttaacagt caggcgcaca ttattatgtg cgggcgtgca
      841 ggatatgagt atgacttttt cgaaggtgat gatggcaaac gccagttggc aaaaaccggt
      901 ataaaaatga aagcagaaac agaaaccggt tatgaaccct ccatccggat ccagatggag
      961 aagcaaatga acatagaaac cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc
     1021 tccacccgta ttgatggaca ggtattttca aacccaacat ttaaaaactt tcttcctcat
     1081 atcgagtctc ttaacctggg aggtgaacac cccggcatcg atacctcacg ggataacagt
     1141 gaattatttg ccaacgacgg tacaccgaca tggctaaaag aaaagcgtgc aaaagaaatc
     1201 gcacttgatg aaattatcga gttgctcaac aagcaccatg ggggcaccag taacgacgcc
     1261 aaacgcgcaa aagctgattt gctggaaaaa acattcacct cccgctcatg ggaaagaatc
     1321 aaaggtatgg actggccgac aatcaaagct ggtcgtaatg cactatggat tgaactggaa
     1381 ggcgttgaat atgcattccc ggatccacaa acacaaaacg aaacgcaaca agcaggatac
     1441 gatgagaata ttccggtgta aaaccaagtg cattttgg
//
LOCUS       DAZSNG010000307         1457 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZSNG010000307 DAZSNG010000000
VERSION     DAZSNG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.314"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHW4Z_004421"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHW4Z_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHW4Z_004422"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHW4Z_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR8883742.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          417 a          326 c          297 g          417 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaa
//
LOCUS       DAZSNG010000308         1383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZSNG010000308 DAZSNG010000000
VERSION     DAZSNG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.315"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            431..600
                     /locus_tag="ACHW4Z_004423"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACHW4Z_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1383
                     /locus_tag="ACHW4Z_004424"
     CDS             746..>1383
                     /locus_tag="ACHW4Z_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8883743.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          340 a          320 c          422 g          301 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 act
//
LOCUS       DAZSNG010000309         1380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZSNG010000309 DAZSNG010000000
VERSION     DAZSNG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.316"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..732
                     /locus_tag="ACHW4Z_004425"
     CDS             <1..732
                     /locus_tag="ACHW4Z_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR8883744.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(734..960)
                     /locus_tag="ACHW4Z_004426"
                     /pseudo
     CDS             complement(734..960)
                     /locus_tag="ACHW4Z_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            1208..>1380
                     /locus_tag="ACHW4Z_004427"
     CDS             1208..>1380
                     /locus_tag="ACHW4Z_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8883745.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQS"
BASE COUNT          370 a          338 c          302 g          370 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg gcatctgtta ctcacatcgc gtctttgttg cgttgaatga aaagcctaat
      361 gtaacagcaa tactttgtgg tggtacgtat cgtgccagaa gtgatgcttt ttacgatgcc
      421 agtaactctt cgccattaga ctctctcaat ccgcgaaaaa tatttatttc cgccagcggt
      481 gtgcataatc actttggcgt cagctggttt aaccctgaag atcttgccac taagcgtaaa
      541 gtgatgaacc gtggactacg gaaaattttg ctcgcccgcc acgcgttgtt cgatgaagtg
      601 gcctctgcca gcctcgcacc gatctctgca tttgacgttc tgattagcga tcgtccgtta
      661 ccggcagatt atgttacgca ctgccagaat ggttctgtaa agatcattac acctgattca
      721 gaagacgaat gacttactga aaaaacacca caatcttgtt aaacatcgtc gggttggact
      781 gattacgttg cactttcacc acatattcca gcttatctat ttggcttatc acctactcca
      841 gacgctggtc atccttgacc agtagccaga tatggctttt gtcgcagtcc tgaatcggca
      901 ggcagagaat gtcttcaacg ttaagagcgc gacgggcaaa agcccacaaa ggtgagtcat
      961 tacacccgga tggtaaacag gcacttcctt gttgtagaag ccgttatatt gtccataatg
     1021 tctgggtttt taatgcatac tgggtaacaa ccggacgaat cttcgaatat cactggcgct
     1081 gccagccggt ttgaattcat ctcacaaccc tgcatagagc gaatctcctg cctgcacgtc
     1141 actctgaatc gccacgggtt taacagacac ctcagagtca tttaagatgg cttaaagaga
     1201 ggtgcccatg agcggtaagc gttatcccga agagtttaaa actgaagcag tcaaacaggt
     1261 tgttgatcgc ggttattctg ttgccagcgt tgcaacacgt ctcgatatca ccacccacag
     1321 cctttatgcc tggataaaga agtacggtcc ggattcttcc actaataaag aacagtcaga
//
LOCUS       DAZSNG010000310         1370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZSNG010000310 DAZSNG010000000
VERSION     DAZSNG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.317"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(166..1353)
                     /locus_tag="ACHW4Z_004428"
     CDS             complement(166..1353)
                     /locus_tag="ACHW4Z_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR8883746.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
BASE COUNT          315 a          414 c          321 g          320 t
ORIGIN      
        1 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
       61 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      121 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      181 catccgggcc agcggctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      241 ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc agcgggtcaa ccttcagtaa
      301 cctctgatac atccctctcc aggtgatttg catcgccgtt tttctcactg tctccgttat
      361 gatgtacacc acttcttcca gtaaccgccg tttcgccgga cttaaaaaaa cgtagtacct
      421 caccatacgg aaccccttat ccgccacatg ccaggagaac ctttccatgg actcatctcc
      481 actcatcaac aggtattctt cccgttttgt tcggtgactg ttgtaacgca gaccgatttc
      541 atcctgaccg gcataatgct ccagacgact catcggcacc ggtggctttt tcaggtaaga
      601 gccaaagtac accgccacat gggtggcatt atccattacc cgggatacgt tgacattcca
      661 gccacggcgg taatgcgtgt ccaggaagcg attccattcc cgtttactgc ttccttctgc
      721 tgccagcgca tccggcatca ccaggtaagg gtatttccgt gacagcaacc gtgttatccg
      781 gtagcgccac atgctcatca ccttacgggc gtaaaaatga agatttttcc aggtgtggcc
      841 cgacgtcaca ccaccggcag ttgtcgataa atggatatgc ggatgccact gctggtcacg
      901 cccccatgtg tggatcaccg tgaatatccc cggctccaca tctgcctgat ggcagatttc
      961 cagtatcaca tccgctgcaa tgcggctcat ctctgccagt aaccaccggt tgtggaacac
     1021 cagggaccag tactggcagg gaagtgtgaa cacaatatgc tgccacgggc agtaggggac
     1081 caggctcagc agatactgta tccactgtgc gccagccttc accccgcagt gcgggcagga
     1141 gcggctttta caccggaagc agaccttttt tgtatggcaa cagtccggtg atgaacaaca
     1201 ccactgcgtg tagcccatca gtgtggtccc gcaggccatg atttttgtca ctgattcaat
     1261 taccaccgga cggactgccc cttccggttg tttctccagc cagttaagcc agcggtttcc
     1321 ctgctgaaaa atatcggcaa aacgggggag catcagaagg gcggggcgac
//
LOCUS       DAZSNG010000311         1356 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZSNG010000311 DAZSNG010000000
VERSION     DAZSNG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.318"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(3..485)
                     /locus_tag="ACHW4Z_004429"
     CDS             complement(3..485)
                     /locus_tag="ACHW4Z_004429"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8883747.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACHW4Z_004430"
     CDS             complement(476..778)
                     /locus_tag="ACHW4Z_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8883748.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="ACHW4Z_004431"
     CDS             1050..1322
                     /locus_tag="ACHW4Z_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883749.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAZSNG010000312         1351 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZSNG010000312 DAZSNG010000000
VERSION     DAZSNG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.319"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..672
                     /gene="rfaY"
                     /locus_tag="ACHW4Z_004432"
     CDS             <1..672
                     /gene="rfaY"
                     /locus_tag="ACHW4Z_004432"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR8883750.1"
                     /translation="FIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGP
                     LVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNF
                     AHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKG
                     LRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKL
                     GLKSK"
     gene            697..>1351
                     /locus_tag="ACHW4Z_004433"
     CDS             697..>1351
                     /locus_tag="ACHW4Z_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR8883751.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     "
BASE COUNT          483 a          183 c          246 g          439 t
ORIGIN      
        1 ttcataaaag ataatgatcc cttttatgag caagttctaa atgacttttt aacttgcagg
       61 gttaaaacat taaaagtttt tcgttctatt gatgatacta aagtcattct tattgacact
      121 gctcgtggac ctcttgtact taaagtttat gcgccaaagc ataaaatgac cgaacgtttc
      181 ttaaaatctt gcataaaaaa agattattat gaaaatttaa tttatcaaac cgatcgggta
      241 cgtggtgaag gcatccaatc aattaatgac tattttttgc tagctgaacg taaaacatta
      301 aatttcgctc attattatat tatgctcatt gagtatattg aaggtgtcgg gttaaatgag
      361 tatttggaaa tttctgaaga tctgaaggat caattatcag aatcgataaa agagttgcat
      421 cagcatggaa tggtgtctgg cgatcctcat aaaggaaatt ttatagtatc cgaaaaaggt
      481 ttaagactaa ttgacctttc aggtaaaaaa acaaccgctg ttttaaaagc aaaagatcgc
      541 attgatcttg aacggcacta taatataaaa aatgaattga aagactttgg ctatacatat
      601 cttatattca aaaagaaaat taaaaaagta atcagggatg taaaagtcaa actaggattg
      661 aagagcaaat aatgattatg atgaagggta attaaattgg attttaaaca tcttactcaa
      721 tttaaagata taattgaact ggacaagcgc cccgttaaac ttgatgaacg ggaaacgttt
      781 aatgtctcat ggggtattga tgagaactac caggttgggg ctgcgatttc aattgcttca
      841 attcttgaaa agaataaaca aaacaaattt acctttcaca taatcgctga ttacttagac
      901 aaagagtata ttgaattatt atcacaatta gcaacgaagt atcaaacagt aattaaatta
      961 tatcatattg attctgagcc attgaaggcg ctacctcaat caaatatctg gccagtatct
     1021 atttattatc gtttgctttc atttgattat ttttctgcgc gattggattc attattatat
     1081 cttgatgctg atatcgtctg taagggttca ttgaacgagt taatagcatt agagtttaaa
     1141 gatgaatatg gggcagtggt aattgatgta gatgctatgc aaagtaaaag cgctgagcgt
     1201 ttgtgtaatg aggattttaa cggtagctat tttaactctg gtgtaatgta tattaattta
     1261 cgggaatggt taaaacaaag actaacggaa aaattctttg atctattatc agatgagtca
     1321 attataaaaa aattaaagta cccggatcaa g
//
LOCUS       DAZSNG010000313         1343 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZSNG010000313 DAZSNG010000000
VERSION     DAZSNG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.320"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..950
                     /locus_tag="ACHW4Z_004434"
                     /pseudo
     CDS             1..950
                     /locus_tag="ACHW4Z_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          301 a          367 c          391 g          284 t
ORIGIN      
        1 ccctgtatcg ccagcagcac attttctccc gaatgggtgt ggagctcccc gtcagtacaa
       61 tggcggatat ggtcggtgtt gccggagctg cactggcccc gctggcgaaa ctgctacgcc
      121 atgagcttct gacacgggat gttattcatg cagatgaaac gtccctgagg ctccttgata
      181 cccggaaagg cggtaaatcc tgctccggct ggctgtgtgc ttacgtcagc ggtgaaagaa
      241 gcggtccgcc ggtcgtctgt tttgactcgc agaccggacg ggccctcaga taccctgaga
      301 cctggctgca gtgctggtgt ggcagtacgc tggtgagtga cggatacagc gtgtacaaat
      361 ctctggcgga taatcacccc ggcatcacat cagcctgttg ctggagtcac gcagggcgcg
      421 gcttcgcaaa tctgtataag gccagcaggg agccccgtgc aggcgtggag ctgagaaaaa
      481 tcgcgggtct ttaccgcatt gagaaactca tccgtgagcg ccctgtggaa aaaatacggc
      541 agtggcgata acggtactcc cggccgatag tgaatgacct gtttgcctgg cttgaagagc
      601 aggagccgtg ctgtccgccc gacgggccat taaataaggc gattaactat attctgaacc
      661 ggcgcgatga actgagctgc ttcctgggtg acggagctgt gcccctggac aacaatatct
      721 gcgagcgtgc cattcgcccg gttgttatgg gacggaaagc gtggctgttc gccggttcac
      781 tgatggccgg aaaccgggcg gcacagataa tgagccttct ggaaacagca aaacgtaatg
      841 gtctggagcc acatgcctgg ctgacggacg tcctgacgcg tctgccggaa tggccggagg
      901 agcgactggc agagttgctg cctcttgagg gatttacctt caccgggtga gtgatacctg
      961 ccgtcaggtg ttcgtgaacc gggctataac ctgcagtcgg ttgttgaacg cctgacaaca
     1021 ggaaatcagc caggccagca accacgcgga ctgacagaga atcagaacct gaatcagcct
     1081 gaaatcatac ccggggcagt acttcatcac cagtcaccaa tacccgtgtc agtacttcgc
     1141 ggcctgacgc gatcacccga ctcagtactt ctgagcccgc ctccggtacc attctctgtg
     1201 ggcccgtaca caacaacaga aaagggtgcc cggcccgggg cgcaaccgta ttacacttcc
     1261 ggaaaatcgt tatacgcaga aaaatcggat cacgctgtgc ctgctttcat gtaatttact
     1321 tacggtgtta taagtatgct aat
//
LOCUS       DAZSNG010000314         1337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZSNG010000314 DAZSNG010000000
VERSION     DAZSNG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.321"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHW4Z_004435"
     CDS             complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHW4Z_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8883752.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(591..1256)
                     /locus_tag="ACHW4Z_004436"
     CDS             complement(591..1256)
                     /locus_tag="ACHW4Z_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR8883753.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          350 t
ORIGIN      
        1 tccatccctc ttctccctgc gcgctactta agtgagtcga ttctatctgc gaaggtgcgc
       61 gaatcaaatt caccggaaat aaacaacaaa aaacccgccg aaacgctgag agatcacagc
      121 gatgggcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagagg tgggcgggga
      181 tgggatcagc tccccacccg gtcactctta cttcctggat tcgtagtcta cgaagacagc
      241 gacctccgtc tggccggttc ggattcgtac ctcacagagg tctttcctcg ttaccagtgc
      301 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
      361 catagcctgc ttctccttgc ctttcggcat gtaagaggct aacctacatg tgcaaagcat
      421 gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta actggggctt
      481 ttctctatct gccttttggt gttcatgcct gaagcagata gcctcaagca cccgcagtca
      541 ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa tcactccttc
      601 tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg agatttgcgg
      661 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
      721 tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca gttataatcc
      781 cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt aacctgctgc
      841 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagcg
      901 gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg gatccctcgt
      961 tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc gtaatcagca
     1021 tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt acgaattaaa
     1081 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gatttccgag
     1141 gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg cagtagtagt
     1201 ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac tgtcataaat
     1261 atgtttattg aggtcataaa aatatcaaag agtgtagcat tagaacgggg ctatcggaaa
     1321 cagatgtgtg actgctc
//
LOCUS       DAZSNG010000315         1330 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZSNG010000315 DAZSNG010000000
VERSION     DAZSNG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.322"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..100)
                     /locus_tag="ACHW4Z_004437"
     CDS             complement(41..100)
                     /locus_tag="ACHW4Z_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8883754.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHW4Z_004438"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHW4Z_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZSNG010000316         1281 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZSNG010000316 DAZSNG010000000
VERSION     DAZSNG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.323"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..93
                     /gene="ospE1"
                     /locus_tag="ACHW4Z_004439"
     CDS             <1..93
                     /gene="ospE1"
                     /locus_tag="ACHW4Z_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR8883755.1"
                     /translation="LKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            483..>1281
                     /gene="ipaH1.4"
                     /locus_tag="ACHW4Z_004440"
     CDS             483..>1281
                     /gene="ipaH1.4"
                     /locus_tag="ACHW4Z_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFR8883756.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          447 a          254 c          217 g          363 t
ORIGIN      
        1 ttgaaattca ctgctacaga aatgcaatgg ttgacaaaga taaatgaagc cggaattgat
       61 gaaaaacaaa gccaacggta ttctgatttc tgaagttaat ttcagactcg tttaagctcc
      121 attttcaaaa aaaatatatc agggtttgtt actaataact tctgaaaaca gaaaaaaata
      181 gagcagtaat tctatttcag agaggagtaa ctccatctat cctcgatacg tactgaacgt
      241 tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga gagacgcaat
      301 gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc agactccttc
      361 ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca aactgtttta
      421 aaattaataa gccatattct atttaactag aattcttatc gttgataaga attctagtta
      481 taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat caaataaaca
      541 atgtatactc gttaactcca ttatctttac ctatggaact gactccatct tgtaatgaat
      601 tttatttaaa aacctggagc gaatgggaaa agaacggtac cccaggcgag caacgcaata
      661 tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta aatttatctg
      721 agttagattt aaaaacatta ccagatttac cgcctcagat aacaacactg gaaataagaa
      781 aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata catgctcaat
      841 ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt aatgcgggtg
      901 ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta cgggttcata
      961 ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg gtagtttctg
     1021 gaaacagatt agacagcatt cccccctttc cagataagct tgaagggctg gctctggcta
     1081 ataattttat agaacaacta ccggaattac cttttagtat gaacagggct gtgctaatga
     1141 ataataatct gacaacactt ccggaaagtg tcctgagatt agctcagaat gccttcgtaa
     1201 atgttgcagg taatccattg tctggccata ccatgcgtac actacaacaa ataaccaccg
     1261 gaccagatta ttctggtcct c
//
LOCUS       DAZSNG010000317         1279 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZSNG010000317 DAZSNG010000000
VERSION     DAZSNG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.324"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHW4Z_004441"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHW4Z_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR8883757.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          230 c          287 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZSNG010000318         1234 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZSNG010000318 DAZSNG010000000
VERSION     DAZSNG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.325"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..323
                     /locus_tag="ACHW4Z_004442"
                     /pseudo
     CDS             1..323
                     /locus_tag="ACHW4Z_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            356..756
                     /locus_tag="ACHW4Z_004443"
                     /pseudo
     CDS             356..756
                     /locus_tag="ACHW4Z_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          355 a          265 c          310 g          304 t
ORIGIN      
        1 aagctgacga gcctgtgctg ggcgctgaaa gaactggcaa aggatatctg gagcagacca
       61 tggagcgagg aaagacgcaa cgactggcag agatggctgc ggccaacagt gacgtcccca
      121 tgatgaagaa tgtggctaaa acgataggga aaaggttgta cggtatcctg aacgccatgc
      181 gccatggcgt atcaaacgga aatgcggaag cactgaacag caagatcagg ctgctgagaa
      241 taaaagccaa gggctaccgc aaccgggagc gctttaagct gggagtgatg ttccactatg
      301 ggaaactgaa tatagcgttc taagccttcc caccatgatc ggggaagccc cagatatgaa
      361 caggcacatt cagagttact ttaaagagct tactgaacgt ctgaggtgtt agagccatga
      421 ctgccgcagc attgctggcg gaaatgcctg aatcgagcag cctctcgaga cgtgaaatca
      481 gcgcacttgt cggtgttgct caggtaaacc gggactcggg aactctgcgc ggtcgacgaa
      541 ccattttcgg tgattgtgct ggggaagaac agctctgtac atggcggcgc ttgaggcccc
      601 ccccggttta atctggtgat aaaggcgttt tacatgcgcc tacttgcagc cggaaacgcc
      661 aaaaaagtgg cactggttgc ctgtatgcgt aagttgctga ccattatgaa cgcaatgctc
      721 agaaaaaacg aagaatggaa tgaatcgtat ttatgaaaac tggattgtgg gccataaata
      781 ttccaaaaag atagcccgct ccgtttatta ccggaacggg cgagagcatg acattgtctg
      841 ttattataat gagactgctg attctactta tttttaattt caagaaaagc gttttctaca
      901 aagaattatg agatttaatg ttttgtttag acaatattat cattctgttc aataatgaaa
      961 gtcttcatca acaacaagta aaaacaacgt ccctctcatc gaaccggact tgcagatttc
     1021 ccgcatccgg ctctcttgca acacatcaga ccttcacgtt cgggcggtta tggggaaggt
     1081 ccagcaagct gtatgagccc taactgtttg aataccagta gattaccaga aacttctatc
     1141 gattgaagac attaccaact agcgttacta aatgttgacc aagttttgtt cacttgtctg
     1201 gttttgatag tagcattctg cagagtattt tgat
//
LOCUS       DAZSNG010000319         1233 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZSNG010000319 DAZSNG010000000
VERSION     DAZSNG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.326"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHW4Z_004444"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHW4Z_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR8883758.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          368 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gtt
//
LOCUS       DAZSNG010000320         1194 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZSNG010000320 DAZSNG010000000
VERSION     DAZSNG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.327"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..496
                     /locus_tag="ACHW4Z_004445"
     CDS             <1..496
                     /locus_tag="ACHW4Z_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR8883759.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>1194
                     /locus_tag="ACHW4Z_004446"
     CDS             560..>1194
                     /locus_tag="ACHW4Z_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8883760.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT          279 a          285 c          387 g          243 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg atgcaaccgt
     1021 cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag caaatgcagc
     1081 cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag aacaggccaa
     1141 aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc tgtc
//
LOCUS       DAZSNG010000321         1175 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZSNG010000321 DAZSNG010000000
VERSION     DAZSNG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.328"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1175
                     /locus_tag="ACHW4Z_004447"
     CDS             <1..>1175
                     /locus_tag="ACHW4Z_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8883761.1"
                     /translation="PETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLDSSWSL
                     FRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSD
                     SPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDE
                     TRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESK
                     NLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLLVSRAS
                     YEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYA
                     GVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQL
                     "
BASE COUNT          249 a          313 c          382 g          231 t
ORIGIN      
        1 ccggagacac gcagtcatga ccgctatgtg acagaacgtg tgacggcgca gagtcacagc
       61 ctgaccctgc ggaatgcgca gggcgaaacg caggtggtgc gtatttcttc cctggacagc
      121 agctggtcgc tgttccggcc ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg
      181 acagggaaaa ttcccggact ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc
      241 agtgaagatg cgatgacggt tgttgtgccg ggacgggctg aaccggccac cctgcctgtg
      301 agcgattcac cgttcactgc actgaagctg gagaacggct gggtggaaac gcccgggcat
      361 tccgtcagcg acagtgcgac ggtttttgcc tccgtcacac agatggcaat ggacaatgcc
      421 accctgaatg gtctggcccg cagtggtcgt gatgtccggc tgtattcctc actggatgaa
      481 acccgtactg cggaaaaact tgcccgccat ccctccttta cggtggtttc tgagcagata
      541 aaggcgcgtg ccggtgagac attgctggaa accgctatca gtctgcagaa agccgggctt
      601 cacacgccgg cacagcaggc cattcatctg gcccttcctg tgctggaaag taaaaacctg
      661 gccttcagca tggtggacct gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt
      721 tttgctgacc tgggagggga aatcaatgcg cagataaaac ggggtgattt actgtatgtg
      781 gatgtggcaa aaggctatgg cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa
      841 aagagcattc ttcgccatat tctcgaaggt aaggaggcgg tcacgccgct gatggagaga
      901 gtacctggcg aactcatgga gaaactgaca tcaggacagc gcgccgccac ccgtatgata
      961 ctggaaacgt ctgaccgttt cacggtggtg cagggctatg ccggtgtggg taagaccaca
     1021 cagttccggg cggtgatgtc agccgtgaac atgctgccgg agagtgaacg tccccgtgtc
     1081 gtggggctgg ggcccacaca ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca
     1141 cagacactgg cgtcctttct gcatgacacg cagct
//
LOCUS       DAZSNG010000322         1171 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZSNG010000322 DAZSNG010000000
VERSION     DAZSNG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.329"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..167
                     /locus_tag="ACHW4Z_004448"
     CDS             <1..167
                     /locus_tag="ACHW4Z_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR8883762.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHW4Z_004449"
     CDS             152..790
                     /locus_tag="ACHW4Z_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR8883763.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1171
                     /locus_tag="ACHW4Z_004450"
     CDS             1018..>1171
                     /locus_tag="ACHW4Z_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883764.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNT"
BASE COUNT          333 a          229 c          312 g          297 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact g
//
LOCUS       DAZSNG010000323         1169 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZSNG010000323 DAZSNG010000000
VERSION     DAZSNG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.330"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            118..660
                     /locus_tag="ACHW4Z_004451"
     CDS             118..660
                     /locus_tag="ACHW4Z_004451"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR8883765.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            887..>1169
                     /locus_tag="ACHW4Z_004452"
     CDS             887..>1169
                     /locus_tag="ACHW4Z_004452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR8883766.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          335 a          223 c          285 g          326 t
ORIGIN      
        1 cgttgacgga cttcggggga aaaacgagta tttttagtca tcctgtttac ctctttctca
       61 ggaagtttag tctccaggat tcccggggcg gttcaggata acaccaaagg cgatgccatg
      121 attggcgtca agcagccttt accaaaagca gttttaagaa ctcagcatga caaaaataaa
      181 gaagcaataa gtatcctgga ttttggtgtt attgatgatg gtgtgacaga taattaccag
      241 gcaatacaaa atgctataga tgccgttgct tcactaccct ccggcgggga gctgtttatc
      301 cctgcgagca accaagcggt ggggtatatt gttggatcca ctttgcttat tcctggcggt
      361 gttaacatca gaggagttgg taaggcatcg caactccgag caaaaagcgg acttacagga
      421 tctgtgttaa ggctgtctta tgattcagac actatcggcc gttatctgag aaatatacga
      481 gtaactggta ataacacctg caatggtatt gacacaaaca ttacagcaga agactctgtc
      541 atcagacagg tttatggctg ggtatttgat aatgtaatgg tgaatgaagt tgaaaccgca
      601 ttatttaatg caagggctct ggcactcaaa atttatagca tgtcaggctg gaacctgtag
      661 agtcggtctt cactttttag gccagtgcgt aagtgttagt gtcagctcct gccatttcag
      721 cagaggaaat tattctgctg atgaaagctt tggcatcagg attcagcctc aaacgtatgc
      781 gtggtcgtca gaggcagtta ggtcagaagc aataatttta gacagtgaga ccatgtgcat
      841 tggttttaaa aatgccgtct atgttcatga ttgccttgat ttgcatatgg aacaactgga
      901 tttagattat tgcggctcaa caggcgtggt tatagagaat gtaaacggag gattttcttt
      961 ctcaaactca tggatagcag cagatgccga tggcactgaa caatttacgg ggatatattt
     1021 tagaacgccg gcctcaacgc agtcacataa aattgtcagt ggtgttcata tcaacactgc
     1081 aaacaaaaac acggctgcaa acaatcaaag tatagcgata gaacagtcgg cgatcttcgt
     1141 ctttgtaagt ggttgtacgt taactggtg
//
LOCUS       DAZSNG010000324         1160 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZSNG010000324 DAZSNG010000000
VERSION     DAZSNG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.331"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="ACHW4Z_004453"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="ACHW4Z_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR8883767.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          247 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAZSNG010000325         1158 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZSNG010000325 DAZSNG010000000
VERSION     DAZSNG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.332"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            163..>1158
                     /locus_tag="ACHW4Z_004454"
     CDS             163..>1158
                     /locus_tag="ACHW4Z_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR8883768.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          314 a          298 c          306 g          240 t
ORIGIN      
        1 gtgaaactgg gctgcatccg actcttttat caacgcttac gtaattaagg gacacagcct
       61 aaatagctgc gcggaatagt agatcccttt gagggaactt agcccggatt gtgcgatctg
      121 atcaatcgcc aaatcaaaac aaatcaccaa ccggactgag caatgccgat catagcacca
      181 atttcccgtg acgaacgacg cctgatgcag aaagccatcc ataaaacaca cgataaaaat
      241 tatgcccgca gactgactgc catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt
      301 gccagaacgc tctgctgcgc ccgttcctct gttggacgct ggattaactg gttcacgcag
      361 tcgggtgttg agggactgaa atcattacct gccgggcgtg cccgtcgctg gccgtttgag
      421 catatctgca cactgttacg tgagctggta aaacattctc ccggcgactt tggctaccag
      481 cgttcacgct ggagtacaga actgctggca ataaaaatca atgagataac cggctgccag
      541 ttaaatgccg gaaccgttcg ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct
      601 gcgccaactc tgcgtatccg tgacccgcat aaagatgaaa agatggcagc aatccataaa
      661 gcactggacg aatgcagcgc agagcatccg gtcttttatg aagatgaagt ggatatccat
      721 cttcatccca aaatcggtgc ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg
      781 ccgggacaga atgaaaaata ttatctggcc ggagcgctgc acagcgggac aggtaaagtc
      841 agctatgtgg gcggcaacag caaaagttcg gcgctgttca tcagcctgct gaagcggctt
      901 aaagcgacat accgtcgtgc gaaaaccatc acgctgatcg tggacaacta cattatccac
      961 aaaagccggg aaacacagag ctggctgaag gagaacccga agttcagggg catttatcag
     1021 ccggtttact cgccatgggt gaatcatgtt gaacggctat ggcaggcact tcacgacaca
     1081 ataacgcgta atcatcagtg ccgctcaatg tggcaactgt tgaaaaaagt tcgccatttt
     1141 atggaaaccg tcagccca
//
LOCUS       DAZSNG010000326         1154 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZSNG010000326 DAZSNG010000000
VERSION     DAZSNG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.333"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..1064
                     /locus_tag="ACHW4Z_004455"
                     /pseudo
     CDS             39..1064
                     /locus_tag="ACHW4Z_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1037..>1154
                     /locus_tag="ACHW4Z_004456"
     CDS             1037..>1154
                     /locus_tag="ACHW4Z_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883769.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          323 a          281 c          268 g          282 t
ORIGIN      
        1 atcttaaatg actctgaggt gtctgttaaa cccgtggcga ttcacaattc tcttgcacca
       61 ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag ttttactggg
      121 ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa tggcagtgct
      181 gggaacctga tgtcgaatgg gtgcgtcagc ccccgcaaga tgcgataacc gctcctgact
      241 ttttctgctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag tttgccgaat
      301 ggttttcaca aaaacgtccg gcggcgatga tgatcggcat ccgtgcggat gagtcctaca
      361 accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat aaaccatgga
      421 ctacggccgc accaggcggt catagctggt acatttaccc catttacgac tggaaagtgg
      481 ctgatatctg gacctgatat gcaaatcatc aaaatctctg caacccactg tataacctaa
      541 tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt ggcccggagc
      601 aacgacaagg attatggctc tgtcacgtta tcgaaccgga tcgctgggct gctatgtgcg
      661 cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgacaat catttctatg
      721 ggcatcggaa aatcctcaaa ccagaacatt tagactggca agaatatgca ttattgctgc
      781 tcaatagcat gccggaaaaa acagctgagc attaccgcaa taaaatcgcc atttatttgc
      841 actggtatca gaaaaaaggc atcgaggttc cacaaaccca gcaaggggac attggcgcga
      901 aagatatccc ctcctggcgg cggatatgca aagttttact caataacgat tactggtgtc
      961 gggcattatc atttagtcct acgaaagcga agaactatca gcgttataac gaacggataa
     1021 aaggaaaacg tcaggaatgg gggatactat gcaacaacga ttaacgcagg atttaacgca
     1081 atttctcgcc agcttgccgg aagatgatcg catcaaagcg attaatgaaa tccgcatggc
     1141 gatccatcag gtta
//
LOCUS       DAZSNG010000327         1091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZSNG010000327 DAZSNG010000000
VERSION     DAZSNG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.334"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHW4Z_004457"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHW4Z_004457"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR8883770.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHW4Z_004458"
     tRNA            complement(986..1062)
                     /locus_tag="ACHW4Z_004458"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZSNG010000328         1072 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZSNG010000328 DAZSNG010000000
VERSION     DAZSNG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.335"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHW4Z_004459"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHW4Z_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR8883771.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          303 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa aa
//
LOCUS       DAZSNG010000329         1069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZSNG010000329 DAZSNG010000000
VERSION     DAZSNG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.336"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(227..652)
                     /locus_tag="ACHW4Z_004460"
     CDS             complement(227..652)
                     /locus_tag="ACHW4Z_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8883772.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHW4Z_004461"
     CDS             complement(649..1032)
                     /locus_tag="ACHW4Z_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8883773.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZSNG010000330         1066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZSNG010000330 DAZSNG010000000
VERSION     DAZSNG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.337"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..190
                     /locus_tag="ACHW4Z_004462"
                     /pseudo
     CDS             1..190
                     /locus_tag="ACHW4Z_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(207..897)
                     /locus_tag="ACHW4Z_004463"
                     /pseudo
     CDS             complement(207..897)
                     /locus_tag="ACHW4Z_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            976..1066
                     /locus_tag="ACHW4Z_004464"
                     /pseudo
     CDS             976..1066
                     /locus_tag="ACHW4Z_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          277 a          245 c          280 g          264 t
ORIGIN      
        1 tacacattta atacgaggga ggccggcaga aaaacggctt gcggtcaggc aggagaaatg
       61 tgtgccgcga atgaagtcct tgtatgactg gataaagctc cagaggaaaa ctttgtcaaa
      121 ccatgctgaa atggtaaagg cgttcaacta tatcctgaat cactggaatg cactgaatga
      181 gttttgccgg gtagtacttc caatcattag aatatgtgct tttcaatgaa ggaaccgatg
      241 actttttcgt ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg
      301 atgcgggggc ggagggtcag gttgttacgt tcatacgttg cgtgaagatt ttgccggtca
      361 ggtgtttctc ctccggcact tctctggcgt agctgcccca gtcatcgctg gtcaccatgc
      421 cgatacagaa cggagtcaac agggctaaca gttcccgaca ggtctcatca ttccgtggcc
      481 cgcaagtgta agccaacaca ccgccagttt tagtgttgta cgcataccag ggccagtgtt
      541 gttgcgcctt gctgccaacg aagcgccact gctcatcaag ttcacagata agggcaacat
      601 cagcgtgagc gactggcgca gatgttatcc gccggggcga gagcttttta atgtccgtat
      661 gacggtattg atgccgattt tcagtgtcct ggcggtatca cggccccctg cgccgttatg
      721 tgccatttca acgatctgct ccttaacgcc gagttttctg gcttcataag agtaagtgag
      781 ctggaaaaca cgtttacatg agcgacaacg gaaacgctca tgccgggaac aactacggcc
      841 atgtcgataa acttcatcgg aattacagcg cggacaatga acagtaacgg tagtcatgga
      901 aaacctcaaa taccagtaga ttaccagaaa cttctatcga ttgaagacat taccttttgc
      961 cgcaataggt ggggtaacat tggtgaaaac gcattacgcc gggtggcagt tggaaaaaaa
     1021 ttatcttttt tcggctctga tcgtggtgga gaaaatgcgg caataa
//
LOCUS       DAZSNG010000331         1066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZSNG010000331 DAZSNG010000000
VERSION     DAZSNG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.338"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(570..749)
                     /locus_tag="ACHW4Z_004465"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACHW4Z_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACHW4Z_004466"
     CDS             843..>1066
                     /locus_tag="ACHW4Z_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8883774.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZSNG010000332         1044 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZSNG010000332 DAZSNG010000000
VERSION     DAZSNG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.339"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHW4Z_004467"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHW4Z_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACHW4Z_004468"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACHW4Z_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          312 a          207 c          177 g          348 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggta
//
LOCUS       DAZSNG010000333         1034 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZSNG010000333 DAZSNG010000000
VERSION     DAZSNG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.340"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..386
                     /locus_tag="ACHW4Z_004469"
     CDS             <1..386
                     /locus_tag="ACHW4Z_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883775.1"
                     /translation="YLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLN
                     YGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSA
                     DNVDGVYYRPLQKLINGTWYNVASI"
     gene            389..934
                     /locus_tag="ACHW4Z_004470"
     CDS             389..934
                     /locus_tag="ACHW4Z_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8883776.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            958..>1034
                     /locus_tag="ACHW4Z_004471"
     CDS             958..>1034
                     /locus_tag="ACHW4Z_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8883777.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKS"
BASE COUNT          292 a          221 c          268 g          253 t
ORIGIN      
        1 tttatttgtg gggtaatcca tccaggcctg ttgttgcaga gcttggtgat gattcaggct
       61 ggcatttttt cagccagaga aacccggata acagcatagt gttcactgtt aacggacagg
      121 taattccgtt aaattacgga aacttcgatg cccgctataa atatcgaaca gagggggtac
      181 aggatgtacg gtatggccat gaaatgtatt acagccccgg cagtaacacc gtttcgtgga
      241 gattttgcgc accttcggga cacgggctgt cagggatggc gatatcggat accggccgta
      301 actcagcgga taacgttgac ggtgtgtatt accgaccact gcaaaaactg attaatggca
      361 cctggtataa cgtagcgagt atttaacaat gttgcattta aaaaatatta ctgcgggtaa
      421 tccgaaaaca gcagaacaat atcagatgac aaaacgatat tcggtcacct ggcttttttc
      481 agaagacgga aaaaactggt atgaagagct gaagaatttc gccagcgaca caataaaaat
      541 agcttacacc ggagatggtc gcgtggtgtg ggtcggtaag gatgtgacag gcatcgagcc
      601 acgcaatgcc agcgttatcg aagttccgga tattaccgct aaccgacgga ttaccgcgcc
      661 gggttactgg ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga
      721 tgagcgtgat gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct
      781 gctgctgggg ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc
      841 gaaatcgctg caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat
      901 tgaatggccc gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg
      961 tctgtagtga tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat
     1021 attattctga aatc
//
LOCUS       DAZSNG010000334         1022 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZSNG010000334 DAZSNG010000000
VERSION     DAZSNG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.341"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            256..>1022
                     /gene="ipaH7.8"
                     /locus_tag="ACHW4Z_004472"
     CDS             256..>1022
                     /gene="ipaH7.8"
                     /locus_tag="ACHW4Z_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR8883778.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          344 a          207 c          145 g          326 t
ORIGIN      
        1 tatgatgtaa aacaaatata cttactcggc ttacatttcc ttgctcactg gaatgttgaa
       61 ttcagtgtta ttattgactg cagccacaat aaaaaaagaa ttccatagaa aacctccata
      121 aataaattac aactaacttc tgttatgtgt aaaatggaaa ctattaaaac ttaatatcgg
      181 aaatggtaag tgaaatttgt ataaatatac aattttaaat atttattctc acaaatataa
      241 ggttgaccta gcattatgtt ctctgtaaat aatacacact catcagtttc ttgctccccc
      301 tctattaact caaactcaac cagtaatgaa cattatctga gaatcctgac tgaatgggaa
      361 aagaactctt ctcccgggga agagcgaggc attgctttta acagactctc ccagtgcttt
      421 cagaatcaag aagcagtatt aaatttatca gacctaaatt tgacgtctct tcccgaatta
      481 ccaaagcata tttctgcttt gattgtagaa aataataaat taacatcatt gccaaagctg
      541 cctgcatttc ttaaagaact taatgctgat aataacaggc tttctgtgat accagaactt
      601 cctgagtcat taacaacttt aagtgttcgt tctaatcaac tggaaaacct tcctgttttg
      661 ccaaaccatt taacatcatt atttgttgaa aataacaggc tatataactt accggctctt
      721 cccgaaaaat tgaaattttt acatgtttat tataacaggc tgacaacatt acccgactta
      781 ccggataaac tggaaattct ctgtgctcag cgcaataatc tggttacttt tcctcaattt
      841 tctgatagaa acaatatcag acaaaaggaa tattattttc attttaatca gataaccact
      901 cttccggaga gtttttcaca attagattca agttacagga ttaatatttc agggaatcca
      961 ttgtcgactc gcgttctgca atccctgcaa agattaacct cttcgccgga ctaccacggc
     1021 cc
//
LOCUS       DAZSNG010000335         1006 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZSNG010000335 DAZSNG010000000
VERSION     DAZSNG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.342"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..189
                     /locus_tag="ACHW4Z_004473"
     CDS             <1..189
                     /locus_tag="ACHW4Z_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR8883779.1"
                     /translation="AYSKLSEETKFIEDLSADSLSLIEMLDMISFEFNLRIDESTLEH
                     IITIGDLISVVKNSTKSI"
     gene            406..>1006
                     /gene="virB"
                     /locus_tag="ACHW4Z_004474"
     CDS             406..>1006
                     /gene="virB"
                     /locus_tag="ACHW4Z_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR8883780.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYY"
BASE COUNT          366 a          178 c          162 g          300 t
ORIGIN      
        1 gcgtacagca aactatctga agaaactaaa tttattgaag atctttctgc cgattctctt
       61 tctctgattg aaatgctgga tatgattagt tttgagttca atctacgtat agatgaatct
      121 acattagaac acattattac tattggagat cttatttctg tagtcaaaaa tagtacaaaa
      181 tcaatatagt aattgataag cattttttca tctatggagc tctcacatca gagctccaca
      241 agaatattat tcttttatcc aataaagata aattgcatca atccagctat taaaatagta
      301 atttttaaga ctaccgttga ctatcatcaa cagttacata caatcttgct cacactgcat
      361 ttaacttttg tcaatataaa tcaatatgaa taaacagggt gtgatatggt ggatttgtgc
      421 aacgacttgt taagtataaa ggaaggccaa aagaaagagt ttacactcca ttctggtaat
      481 aaagtttcct ttatcaaagc caagattcct cataaaagga tccaagattt aaccttcgtc
      541 aaccaaaaaa cgaatgtacg cgatcaagaa tccctaacag aagaatcatt agccgatatc
      601 ataaaaacta taaagctaca acaattcttc cctgtaatag gaagggagat tgatggtaga
      661 attgaaattc tggatggcac tcgtagaaga gcatctgcaa tatatgcagg agcagatctt
      721 gaagttctat attcaaaaga atatatatct actcttgatg ccagaaaact agcaaacgat
      781 atacaaacag caaaagagca tagcatccga gaacttggta ttggtcttaa ttttctgaaa
      841 gtatcaggga tgtcctataa agacatagcc aaaaaagaga atctgtctcg cgcgaaagtc
      901 actcgtgcct ttcaggcagc aagcgttcca caggaaataa tatctctatt tccaatcgcg
      961 tcagaactta actttaatga ctacaagata ttattcaatt attata
//
LOCUS       DAZSNG010000336         1004 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZSNG010000336 DAZSNG010000000
VERSION     DAZSNG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.343"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..529)
                     /gene="mxiE"
                     /locus_tag="ACHW4Z_004475"
     CDS             complement(<1..529)
                     /gene="mxiE"
                     /locus_tag="ACHW4Z_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR8883781.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYA"
     gene            complement(659..>1004)
                     /gene="mxiM"
                     /locus_tag="ACHW4Z_004476"
     CDS             complement(659..>1004)
                     /gene="mxiM"
                     /locus_tag="ACHW4Z_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR8883782.1"
                     /translation="SSNSEKEWHIVPVSKDYFSIPNDLLWSFNTTNKSINVYSKCISG
                     KAVYSFNAGKFMGNFNVKEVDGCFMDAQKIAIDKLFSMLKDGVVLKGNKINDTILIEK
                     DGEVKLKLIRGI"
BASE COUNT          334 a          205 c          122 g          343 t
ORIGIN      
        1 atgcataccc attattcatg gcagctgaag tgattgtttg attgtgcaaa aatacatcta
       61 ataaaccatt tactaaccgc caagtgttca gttgttcttt gacttttgcc cctaaagctt
      121 ttctacataa agatctaaaa taagcctccg acacaccata atgctctgtc agactcttta
      181 ttcttacctc cttttctttc tcaatagttc tcagaagata aagaaccaaa tgatatgact
      241 cagtcattcg tatcatagcc agtgcattac ttacattctg gtcagatgaa aagatgaatc
      301 ttaacattaa cttatcaagc atcgattcgg tattacaact atcccgaaga tcattctcat
      361 gatagaaatg aatacatttt tcagcatggc tatgctctac tagagcacca ctgaaatagt
      421 cccaaaaagc aagaaatttt gcacaactat taatgttaaa gcgataatga tagttcacat
      481 tatcactgaa ttttatattt tgatttcgga caaaaaaaaa cccttccatg ctggagccag
      541 ctcaattatg ttacgttctt tatcttttac aagctcaacg ttaactggtt catgtccact
      601 agagtaaatg taaaacatat tacttgtatt tagaccttta tatttactca caatacaatt
      661 atatccctcg aattaatttt aatttaactt ccccatcctt ctctataagg atggtatcat
      721 ttatcttatt accttttaaa acaaccccgt ctttcagcat agaaaatagt ttatctatag
      781 ctatcttttg tgcatccatg aagcacccat ctacttcctt aacattaaag ttgcccatga
      841 atttacctgc attaaaacta taaaccgcct taccactaat acatttagag taaacattta
      901 tacttttatt ggttgtatta aacgaccata ataaatcatt tggaatagaa aaataatcct
      961 tacttacagg aacaatatgc cattcttttt cagaattaga tgat
//
LOCUS       DAZSNG010000337          973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZSNG010000337 DAZSNG010000000
VERSION     DAZSNG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.344"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            7..756
                     /gene="phoC"
                     /locus_tag="ACHW4Z_004477"
     CDS             7..756
                     /gene="phoC"
                     /locus_tag="ACHW4Z_004477"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR8883783.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(811..915)
                     /locus_tag="ACHW4Z_004478"
                     /pseudo
     CDS             complement(811..915)
                     /locus_tag="ACHW4Z_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          270 a          210 c          214 g          279 t
ORIGIN      
        1 tgttatatga aaagacagct ttttactctt agtattgtcg gggtattttc tttaaatacc
       61 tttgcatcat tccctccggg aaatgatgtg acaacaaaac ctgaccttta ctacctgaca
      121 aacgataatg ctattgacag tctggcatta ttaccgcctc caccacaaat cggaagtatt
      181 gccttcctga atgaccaggc catgtatgaa aaggggcgtt tattgcgaaa tactgaaaga
      241 gggaagctgg cagctgagga tgccaatctc agtagtggtg gcgtggcaaa tgtattttct
      301 gctgcttttg gttctccgat aacggctaaa gattcaccag aattgcataa attactgaca
      361 aatatgattg aggatgccgg agacctggca acgcgttcag caaaagaata ttatatgcgt
      421 attcgacctt ttgcttttta tggtgtttct acgtgtaata caaaagaaca ggatacatta
      481 tccagaaatg gctcttatcc atcaggtcat acatcgattg gttgggcaac agcacttgta
      541 ttgtccgaaa taaatccggc acgccaggat accattctga aacggggtta tgaactgggg
      601 gacagcaggg ttatttgcgg ttatcactgg caaagtgatg ttgacgcagc acgtattgtt
      661 ggctctgcta ttgtagcaac actgcactca aatcctgtgt tccaggcaca attacaaaaa
      721 gcgaaagatg aattcgctaa caatcagaaa aaataatgct tttagccagt acgccttata
      781 accccgacct tcagggctaa tgccagtcag ttaagcaact gactggctct ttttcagggc
      841 tgtggggtat ttccagtgcc tctcctttac cactctcgga aaggcccttt cccttcttgt
      901 cggtaatttc actaggctgt gtcccttaat tacgtaagcg ttgataaaag agtcggatgc
      961 agcccagttt cac
//
LOCUS       DAZSNG010000338          935 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZSNG010000338 DAZSNG010000000
VERSION     DAZSNG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.345"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..222
                     /locus_tag="ACHW4Z_004479"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHW4Z_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            365..586
                     /locus_tag="ACHW4Z_004480"
                     /pseudo
     CDS             365..586
                     /locus_tag="ACHW4Z_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(607..669)
                     /locus_tag="ACHW4Z_004481"
                     /pseudo
     CDS             complement(607..669)
                     /locus_tag="ACHW4Z_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          298 a          213 c          204 g          220 t
ORIGIN      
        1 ccatgacaaa ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat
       61 atgccggcag cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt
      121 tatgggggcg gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag
      181 ttacacagtt gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc
      241 gggaaaccgg tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc
      301 gacaaacatc caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga
      361 ggaaatggac acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca
      421 acaggacact atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc
      481 ttatgcttcc gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt
      541 cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc
      601 gatctacgcc acacaggctc tgggttgcag tgactgctcc attaaggcga ttttgaactc
      661 ataagggaag taacaaaaaa actgcaaaag tacaataatt atatatggcc aaactaacct
      721 ctattaaaaa acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat
      781 ttcattgcag aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag
      841 tatcgttttt ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt
      901 atgaaaataa tttcacaaaa aagattaact tgagt
//
LOCUS       DAZSNG010000339          932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZSNG010000339 DAZSNG010000000
VERSION     DAZSNG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.346"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(55..243)
                     /locus_tag="ACHW4Z_004482"
     CDS             complement(55..243)
                     /locus_tag="ACHW4Z_004482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883784.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHW4Z_004483"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHW4Z_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DAZSNG010000340          927 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZSNG010000340 DAZSNG010000000
VERSION     DAZSNG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.347"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            175..771
                     /gene="fimE"
                     /locus_tag="ACHW4Z_004484"
     CDS             175..771
                     /gene="fimE"
                     /locus_tag="ACHW4Z_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR8883785.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          270 a          168 c          205 g          284 t
ORIGIN      
        1 aatagatgtt gtcacatcag tgatatttta tttttgtatg atgtttaatg taattgactg
       61 atagccacat aacttcgtgt gtggttatct ttttatctat tgggctaatt ttgaccgatt
      121 gaggtttcct ataggtattc attcaaatat atctcagtta ggagtactac tattgtgagt
      181 aaacgtcgtt atcttaccgg taaagaagtt caggccatga tgcaggcggt ttgttacggg
      241 gcaacgggag ccagagatta ttgtcttatt ctgttggcat atcggcatgg gatgcgtatt
      301 agtgaactgc ttgatctgca ttatcaggac cttgacctta atgaaggtcg aataaatatt
      361 cgccgactga agaacggatt ttctaccgtt cacccgttac gttttgatga gcgtgaagcc
      421 gtggaacgct ggacccagga acgtgctaac tggaaaggcg ctgaccggac cgacgctata
      481 tttatttctc gccgcgggag tcggctttct cgccagcagg cctatcgcat tattcgcgat
      541 gccggtattg aagctggaac cgtaacgcag actcatcctc atatgttaag gcatgcttgc
      601 ggttatgaac tggcggagcg tggtgcagat acccgtttaa ttcaggatta tctcgggcat
      661 cgaaatattc gccatactgt gcgttatacc gccagtaatg ctgctcgttt tgccggatta
      721 tgggaaagaa ataatctcat aaacgaaaaa ttaaaaagag aagaggtttg atttaactta
      781 ttgataataa agttaaaaaa caaataaaca caagacaatt ggggccaaac tgtccatatc
      841 ataaataagt tacgtatttt ttctcaagca taaaaatatt aaaaaacgac aaaaagcatc
      901 taactatttg atatgtaaat tatttct
//
LOCUS       DAZSNG010000341          926 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZSNG010000341 DAZSNG010000000
VERSION     DAZSNG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.348"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            103..>926
                     /locus_tag="ACHW4Z_004485"
     CDS             103..>926
                     /locus_tag="ACHW4Z_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8883786.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVHVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNS"
BASE COUNT          313 a          184 c          160 g          269 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtac atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta ttctggtcct
      901 cgaatatttt tctctatggg aaattc
//
LOCUS       DAZSNG010000342          922 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZSNG010000342 DAZSNG010000000
VERSION     DAZSNG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.349"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..199
                     /locus_tag="ACHW4Z_004486"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHW4Z_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            299..>922
                     /locus_tag="ACHW4Z_004487"
     CDS             299..>922
                     /locus_tag="ACHW4Z_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8883787.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          292 a          217 c          205 g          208 t
ORIGIN      
        1 ttggctacgt aattaaaaca gaaaataaag aaaccccatt cactgtagtt gatatcgacg
       61 gtctatcagg aaactttaaa actcttgttg aaggtgccac aaaaatgtgc ctagtttaca
      121 tcggaaataa tctgcccgca gaaaaaaagc cgaatttctg gcaaatctga ttgcaatgaa
      181 attaaaaggt gaaatctgaa aaaaagaaag cctgcacagt gtgcaggcct gagtgaagaa
      241 cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt aaaaaacaat
      301 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      361 cgtcctgtgg gcaagcgacg attttgaatc gaccagcacc actctggact atctgctcgt
      421 taagtcaggt aaaaaactga gcaactattt taaagctgtt gccacaaatt ttcctgtcgt
      481 taatgacctt ccccctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
      541 caaagactcc atgacatggg aactaaaacc gggagcagcg ccagacgacg ttcaccacca
      601 ggataatgct caagaaacca aagaactggc gggaggccag gaagaaaacg cgcaggcaga
      661 cgcccacgag gattgccagg attgcgaagt ctctgtagcc actttgcggt tcacacagcg
      721 tcttctgcac atttttacgt atgctgccgg ggatctgaaa tacctgcatc atgccacccg
      781 tgaacaacgc aaacacatta ctgctcttga gatggatcag gaaaacagct atgtccagaa
      841 tctgctgttg gccatacgcg gcatggcaga accgacaact ctggataatg ccgccctgct
      901 ccgcctgact gatgcaatta ag
//
LOCUS       DAZSNG010000343          901 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZSNG010000343 DAZSNG010000000
VERSION     DAZSNG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.350"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="ACHW4Z_004488"
     CDS             complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="ACHW4Z_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR8883788.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IY"
     gene            complement(248..378)
                     /locus_tag="ACHW4Z_004489"
                     /pseudo
     CDS             complement(248..378)
                     /locus_tag="ACHW4Z_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            376..459
                     /gene="tnpB"
                     /locus_tag="ACHW4Z_004490"
                     /pseudo
     CDS             376..459
                     /gene="tnpB"
                     /locus_tag="ACHW4Z_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          260 a          157 c          209 g          275 t
ORIGIN      
        1 cgtatatagc tgctttttta ttgaaaacag tatatccatc tgtagtaaca gtagaagata
       61 ataatacagg atttgatact tcaaggatga tattttcttg tttttgtgga agacaaggga
      121 atatagtttg tgtaagcata ttacttcctt atgttatatt ttggtaaaaa atttattttt
      181 ttacattata gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt tcgggtggcg
      241 cttccaatca gtagaatatg tgtttttcaa tgaaggagcc gatgactttt cgtggatttc
      301 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
      361 caggttgtta cgttcaagat atagcatgtg ctggatgaag atcccttttc tggtcacctg
      421 tttatctttc gtggccgacg gggtgacact gttaaaatgt aatgaacgca tcccacacaa
      481 ttaaacacgg agtagtatga tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag
      541 ctttccggca tcatactgcc caatacggga tgcataaagc gtcctcagac tggagactcc
      601 acaaatgaaa tatacaccgg ttggcgttga tatggcaaaa catgtcattc agattcactt
      661 catcaatgag cacatgtcag cgccagtgat ataagacggt aatggggatg cggacgattt
      721 cctggactct cagaagatga aacatgttta ctgaagaaca aaaaattcgc gctattgagc
      781 tttatttcaa atatggtaag aagcttgagg tgtactggca atagcggaca ccaccatttg
      841 ttctttttct aagcagtcat ctgataatat tttatcctga aggctgccgg ggagatattc
      901 c
//
LOCUS       DAZSNG010000344          893 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZSNG010000344 DAZSNG010000000
VERSION     DAZSNG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.351"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..143
                     /locus_tag="ACHW4Z_004491"
     CDS             <1..143
                     /locus_tag="ACHW4Z_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8883789.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHW4Z_004492"
     CDS             127..486
                     /locus_tag="ACHW4Z_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883790.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHW4Z_004493"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHW4Z_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR8883791.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..>893
                     /locus_tag="ACHW4Z_004494"
     CDS             707..>893
                     /locus_tag="ACHW4Z_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883792.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQAT"
BASE COUNT          280 a          194 c          227 g          192 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cac
//
LOCUS       DAZSNG010000345          880 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZSNG010000345 DAZSNG010000000
VERSION     DAZSNG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.352"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..784)
                     /locus_tag="ACHW4Z_004495"
                     /pseudo
     CDS             complement(1..784)
                     /locus_tag="ACHW4Z_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            792..>880
                     /locus_tag="ACHW4Z_004496"
     CDS             792..>880
                     /locus_tag="ACHW4Z_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883793.1"
                     /translation="MILWSVKKTDIRRGRHCVFLMHAHRVFVT"
BASE COUNT          192 a          215 c          219 g          254 t
ORIGIN      
        1 cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga
       61 tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga
      121 ccttgcggct taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt
      181 ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca
      241 gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac
      301 tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct
      361 gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct
      421 gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc
      481 aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg
      541 actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc
      601 actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat
      661 tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc
      721 tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt
      781 tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact
      841 gtgttttcct gatgcatgct caccgggtct ttgttaccaa
//
LOCUS       DAZSNG010000346          870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZSNG010000346 DAZSNG010000000
VERSION     DAZSNG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.353"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>870
                     /locus_tag="ACHW4Z_004497"
     CDS             <1..>870
                     /locus_tag="ACHW4Z_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR8883794.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZSNG010000347          858 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZSNG010000347 DAZSNG010000000
VERSION     DAZSNG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.354"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHW4Z_004498"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHW4Z_004498"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR8883795.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          230 a          212 c          188 g          228 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatcgttaat aattaaaggg agtaacgtat tatgtcagaa
      841 gaaaataaag aaaatgga
//
LOCUS       DAZSNG010000348          855 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZSNG010000348 DAZSNG010000000
VERSION     DAZSNG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.355"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          283 a          144 c          161 g          267 t
ORIGIN      
        1 tgccaagcct ctttattata agtaagatct ggcactggaa tattctcaaa aggactaaca
       61 tcatcaccac gaccttcaac caaccatttt attgcattta aatcccagaa tagttcagta
      121 gtggtcaatt tgggaaacgc acaatattgt acggttacgt tatcgcaata gagtgaactc
      181 tcgccctata cggcgttttc aaggattagt ccttaatcgg acaaccacat taaagcataa
      241 taaataatta agctttaata atatactata gatacagttc tattataaaa aatactattt
      301 taggtgatgc attaaattag tagaatattt attcttttat aaaagcacca atgaattgtc
      361 caagtaaaca tgagataaaa tgtgtagagg ataactgagt ttggtcactt taactgtatt
      421 agtcgcagtc tgactctcga ctttaaaagg atgggaatta cctgtactga tagtgagaaa
      481 gctttccatt gaaataagtc aaataaaagt tgatggcttc agtgctaaag tgaggtgccc
      541 gctagccgta ttcactcgaa tcggaattca atgtattgct tctgccattt ccataatatt
      601 tcacgggaaa aacaaaatct gaaaaactat acttaagtgt tgtattgagt cagttgagat
      661 acaaatacat atttatcagc acacgttacg tcaaatacag gaggttgtca tgttggtact
      721 gaaaggcacg ttggctattg cagccgtgat gacaatttat tgtcttatcg ttgttcttat
      781 ggaacgcttt tctgattgat ttcatattgg cgagagaaca agaaggtgag tagaattgtc
      841 gcggaatcct gaaac
//
LOCUS       DAZSNG010000349          853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZSNG010000349 DAZSNG010000000
VERSION     DAZSNG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.356"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..81)
                     /locus_tag="ACHW4Z_004499"
     CDS             complement(<1..81)
                     /locus_tag="ACHW4Z_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8883796.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSI"
     gene            complement(146..592)
                     /locus_tag="ACHW4Z_004500"
                     /pseudo
     CDS             complement(146..592)
                     /locus_tag="ACHW4Z_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT          250 a          204 c          161 g          238 t
ORIGIN      
        1 gatcgaaagt tgattttcaa gaaccgttct gacagcttcg gctttgaact ctttagagta
       61 acgttgggtt tttctgctca ttattagctc cttctgatgc cattctattt caggaaggag
      121 tgtccgttaa actcaggcta cctcaaacat catctggata atcggcatga gcaataacat
      181 gcttgccggt gcgactgtgt acttcaagaa taaagggcaa atgctgacat ttttccagcg
      241 ccgttttcgc ttgtttctgt tgattatctg ccagcgcaat aaaccagtcg ccgccattca
      301 tcaaccacaa agacatctgc tggaatgcca gcgcatccat cgccatctgt tcatgattgc
      361 ctcttaccgc acgaacccaa tgttgttcca gtaactgcag acaacgtaaa ctttgcggcc
      421 cacgatcgat aacgtctccc actgagataa gtaaatctcg ccacggatca aaacgacaat
      481 gccataattt gcggcgcaac tgctcaagac aaccgtgtat atcgccagaa agccagatat
      541 gtcgccattg atgacccgca attctctgat aaacgggcgc aggctgtttc atcaatattt
      601 tcctcccgcg ctaaagatca cataatctta acaagaatgt taaaaaacgc tggactcaga
      661 cagtagagtg tgtgttatgg ttgactataa agtcagcgaa ggaaatgctt ctggctttta
      721 acagataaaa agagaccgaa catgattcct gttttcgtca acaaacaata aaacccttta
      781 aaattaatgc gttgaataaa tttacgttca tcttttaatc cctgacacgt accattgcat
      841 attaatacat tca
//
LOCUS       DAZSNG010000350          832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZSNG010000350 DAZSNG010000000
VERSION     DAZSNG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.357"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..327
                     /locus_tag="ACHW4Z_004501"
     CDS             <1..327
                     /locus_tag="ACHW4Z_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8883797.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACHW4Z_004502"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACHW4Z_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR8883798.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DAZSNG010000351          819 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZSNG010000351 DAZSNG010000000
VERSION     DAZSNG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.358"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(135..536)
                     /locus_tag="ACHW4Z_004503"
                     /pseudo
     CDS             complement(135..536)
                     /locus_tag="ACHW4Z_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          199 a          199 c          181 g          240 t
ORIGIN      
        1 atagtcggct aaatgaagtt tactgtaaaa aaacgatact tttttcgtaa gcgatgagat
       61 gtggagccag aaaaatggct gcgttacgtc attgaactgt ccttgaccca cgatattcag
      121 acagtcgttg tatcttaagt tgacattacc cgctgacgtc gatattccct tggtgagaga
      181 taacccaacg cactatgcgg atggttttca ttgtaatgct cgatcgccac tgcaaggtta
      241 tgcaatgccg ttcttggttt cggcatgaac gcgatgcagt cttccttcat cgttttcatg
      301 aaccgttctg ctattccatt actctgaggg ctactgactg ccgtcgtgca gggctccaga
      361 ttcaactctc tggcgaactg ccgtgtttca tgagccgtat aagccgaacc gttatcggcc
      421 actggattgg ccgttccggt cctttatcgc catatcgctc ctgacatgac atcctgcacc
      481 gttgctttgt cataatctcc cgtgcctgct gcccagtcta tggcttcacg atcgcaatat
      541 ggaacgtaac gctgtcatcg ggagaatcgt caatgcggca ttcggttaac gcttaccaat
      601 atcaacacca accggatttg tggagcctcc agtctgaagg ggctttatgc gttccgtata
      661 ggatattatg atgctggagt tttgcaaagc tccactcctt tccgaacatt tacacagata
      721 ttgcctaaga aaagtgaaac cataatatgg ttatagtccc tttcagtgca aatacttagc
      781 ttgttgcaca gagaaataga agctgcataa gctctttct
//
LOCUS       DAZSNG010000352          809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZSNG010000352 DAZSNG010000000
VERSION     DAZSNG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.359"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            192..665
                     /locus_tag="ACHW4Z_004504"
     CDS             192..665
                     /locus_tag="ACHW4Z_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883799.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          252 a          166 c          177 g          214 t
ORIGIN      
        1 cgttacagac ggtcccggca aaagtgcctg acctgacccc tattagcggt taccagcgcc
       61 ccaccagacc cggtgacatc accccccgga tcatggacga ctgggggcag taatcatgcc
      121 gggtctggct ggctaacacc ccattataag gggtacgaat aaagtaacgg gggggagatg
      181 aaccctatgc aatgtcctgg ttaatatctc agtgtgctca tcagtgcact gataataaaa
      241 agacggagac agatgccatt tacgataaag ttcggagttc ttatttgctt tcatgtattc
      301 tgaaaaaaaa caaaaatgtt gggcttattc ttcatgcacc atcatttgtt tctgtgtctg
      361 aaaaaatcgc cagaattgtg atggcaaatt actcccgtaa ctggagtaat tccgagttgg
      421 ccagtgcggt tttaatgagt gaatcttctt taaaaagaag aatgtacaaa gaggtcggtt
      481 ctataagtac atttgtacat aaaataaagc ttacagaagc tatcaggaaa ttacgaagga
      541 caaatactcc catttctgtc atatcatctg agttaggtta cagttcacca tcgtattttt
      601 ctaaagtctt ctttaaatat ttaaagacat accctcaaaa cataaggaag aaaaatggtc
      661 ggtaatgtca ccaattagga ggacttgcag agccgctttt ttacgatact gagatcaaac
      721 agacataatc tatgcggaga tttacagcag ggcatggttc acaaatctga cagcgatgaa
      781 ttatcggcac tgcgggcaga aaatgctcg
//
LOCUS       DAZSNG010000353          803 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZSNG010000353 DAZSNG010000000
VERSION     DAZSNG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.360"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            46..803
                     /locus_tag="ACHW4Z_004505"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHW4Z_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZSNG010000354          780 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZSNG010000354 DAZSNG010000000
VERSION     DAZSNG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.361"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            60..780
                     /locus_tag="ACHW4Z_004506"
                     /pseudo
     CDS             60..780
                     /locus_tag="ACHW4Z_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          254 a          115 c          151 g          260 t
ORIGIN      
        1 attgtaaatg acttagattt aaataagata ataattttgt taacaatatg aggcgggaga
       61 tgttttctac aaacttaata cagagtaatt atggtgattt aaatattaaa agcctggctt
      121 tcgactcttt taaagaaagg cttgagtcta ccatggcggc actcactttc tttatatcaa
      181 cgggtcaatg tgactgcgat gaaatagcag agtcgaactt caatcgtatg attgcctata
      241 tgtctaacat taactatgat gcctcaaagc taggagcgcc agcacttagt tttgatacgt
      301 atctgcagga caatgttaaa tatagagtga taattaataa tctttatggc agtgagtttc
      361 ggataagaga tattaatgag aattttgttg ggatggattt tacctgtgaa ttccgacccg
      421 aaaaaatgac gaatttgatc aatataaaaa ataggttggt tattcattat ttaaggacta
      481 tatactatga aaaaaattat atacatcctg ccgggagcat ttttgctgcg attcaaaaaa
      541 aatgagagtc tcctaaagtt tccttcgata atatcaatgc ttaatatcaa tcttcttttt
      601 aatccactaa atcttccggg aatgggaagt ggtgtgcttg aagatattat gagtattcca
      661 gatagttcac ttagaaaacg tctcgggtat gaggttttaa gcttctcact acaggcacat
      721 tcattgagcc aggaatgtat tgataagttg gacatttttt tcgctgacga tctgtttaaa
//
LOCUS       DAZSNG010000355          779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZSNG010000355 DAZSNG010000000
VERSION     DAZSNG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.362"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..663)
                     /locus_tag="ACHW4Z_004507"
     CDS             complement(<1..663)
                     /locus_tag="ACHW4Z_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR8883800.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IAL"
     gene            complement(653..>779)
                     /locus_tag="ACHW4Z_004508"
     CDS             complement(653..>779)
                     /locus_tag="ACHW4Z_004508"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR8883801.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          175 g          150 t
ORIGIN      
        1 cagtgctatt ttgattcgac gttgatcaag tttctgataa ttccaacgac ggtcgacggg
       61 caccatccag tcaatcaaca tacctaccgc cagccctgtg atgacatcaa taaaatgatg
      121 ctgccatgtc gtcagcgtcg aaatggcgat gagtaaaaac catccgccac agactttacg
      181 ccacctcaca gccagatgct gacgaaagtg acgccaaagt agccagcaga gaataatatg
      241 cagcgaagga gactggttat aaggcagatc aaacagttca agttgcgaaa atagccatcc
      301 tgtcacccca ctcacttcag gacggataaa actaaacttc agcgggtaga gaaaaaaacc
      361 gcagcaggcc attaccgttg ccagaataag ccggtggaca agtcggcgtt gttcgaatgt
      421 cgagctacaa acgaacagcg aaaatccata taaaagatcc agactccagt aaggaacaat
      481 agtccaggga agaaaaggga tcgccgtttc ccaaccgaat acctgactgg ggatatcatg
      541 gctgttaagg tcctgaaccg cggtgaactg attaagagat ccataggtga agaagaaaaa
      601 cggggccagc aacaataacc agccagcgcc ttgtagcaat acgttccgac gttcagttat
      661 cacgacgcac cgccatcgaa accgtaaaaa tgccccacac atcaatccgt tgagtgcatt
      721 tatcaaatcc ggcattacgc acgagcgaat ccatctcccc ttgcgaacgc acgcgcatt
//
LOCUS       DAZSNG010000356          759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZSNG010000356 DAZSNG010000000
VERSION     DAZSNG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.363"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>759
                     /locus_tag="ACHW4Z_004509"
     CDS             <1..>759
                     /locus_tag="ACHW4Z_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR8883802.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZSNG010000357          742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZSNG010000357 DAZSNG010000000
VERSION     DAZSNG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.364"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            105..623
                     /locus_tag="ACHW4Z_004510"
                     /pseudo
     CDS             105..623
                     /locus_tag="ACHW4Z_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          180 a          204 c          178 g          180 t
ORIGIN      
        1 aaactctgac cacaatctgc cggttacgcc gaatctgctg aatcagaact ttacgcccac
       61 agcacctaat caggtctggg tcgcagattc agttgtccag gccttccgaa atcaaccgac
      121 tgaaggcgct ggtcgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc
      181 gcgcaaaaac cgaacggtgg atacaggaag cacaggagcg aatcagcgca cttcaggagg
      241 aaatggcgga aacactgggt gagcaatatg acccggtact gccatcttcc ctgcgccagt
      301 cttcagcccg taaaccgtta ccagcctcac ttccccgtgc cccccgggtc atccggccgg
      361 aagaggaatg ctgtccggcc tgtggtggtg aactcagtcc actgggatgt gatgtgtcag
      421 agcagctgga gcttatcagc agcgccttta aggttatcga aaaacaacgc cctaaactgg
      481 cctgtcgccg gtgcgaccat atcgtgcagg caccggtgcc ttcaaaaccc attgcacgca
      541 gttatgccgg agcggggctt ctggcccatg ttgttaccgg aaagtatgca gaccatctgc
      601 cattataccg ccaaagtgat ctactattcc acacagctat ttagctctct ttttgttgag
      661 ctcccatgat tctttcattt attgttacta attattttat gttatcatga tgttagaaca
      721 cgctctgctc tgtgtctcta tt
//
LOCUS       DAZSNG010000358          722 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZSNG010000358 DAZSNG010000000
VERSION     DAZSNG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.365"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            431..506
                     /locus_tag="ACHW4Z_004511"
     tRNA            431..506
                     /locus_tag="ACHW4Z_004511"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:464..466,aa:Ile,seq:cat)
BASE COUNT          224 a          115 c          164 g          219 t
ORIGIN      
        1 taaatctgtt ttttaattat cgatgtggtc tggagaggca actgtcaaaa atcgcgcata
       61 cgaaactaat gattagcaga ataagatcta tgaaagaata tattgctata tgaagtttat
      121 ttttatggag tgaaaagtaa cagatatcat aatgatgcga aaactcttca gctaatgtaa
      181 taaagttata ttattagata caaaatcatt acaaagtatc attgaatcga tgataactat
      241 agatagtgct aattattaac atttgcttgt tgactgcgag tatcgacttt gcgaatggag
      301 gcggtgtcgg gttttatgtc gcgtgtaatc acgacccaag gtgaatggga acgcataaaa
      361 atctgttatt tacatcataa gatcatcatg tttattgcgt ggtttataag tattgttatt
      421 gcatacttaa ggccctttag ctcagtggtt agagcaggcg actcataatc gcttggtcgc
      481 tggttcaagt ccagcaaggg ccaccaaatc atcattcatg tatcaggaag agttaagggt
      541 acaaggggta ctcgatggcg gtctatattt catgactaac gccatttcaa ggaagaagtg
      601 cggtgggtgg tttcgaatga gggctggtgt tttggtacgt ggcacgccac tgcaatgtat
      661 ttattcatca gtactcattc aaaagtacct ggaaaaaatg cccgctatgc caagcgggca
      721 aa
//
LOCUS       DAZSNG010000359          696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZSNG010000359 DAZSNG010000000
VERSION     DAZSNG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.366"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            32..493
                     /locus_tag="ACHW4Z_004512"
     CDS             32..493
                     /locus_tag="ACHW4Z_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR8883803.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>696)
                     /locus_tag="ACHW4Z_004513"
     CDS             complement(534..>696)
                     /locus_tag="ACHW4Z_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8883804.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          160 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatg
//
LOCUS       DAZSNG010000360          693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZSNG010000360 DAZSNG010000000
VERSION     DAZSNG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.367"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(4..>693)
                     /locus_tag="ACHW4Z_004514"
     CDS             complement(4..>693)
                     /locus_tag="ACHW4Z_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR8883805.1"
                     /translation="ATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNE
                     IAAVVAERDIEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDP
                     DWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVG
                     PTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIF
                     NYLKYYKEKCF"
BASE COUNT          222 a          126 c          101 g          244 t
ORIGIN      
        1 tctttagaag catttttctt tataatactt taaataatta aaaatgcctg ccatataatg
       61 cttttgttta aatttatgtt ttgcacaata ccgatactga ttgctattta caggtttaag
      121 taaatcttct ttactccacg gagaagctgc tttggcgatc aaaaaactac gtgaaaccgg
      181 ataatcagcc cactcatgcc agggttttgt aggtcccaca tagtgtataa acacggtgtc
      241 atcattgact ggattatcaa ctttgtcttt taattcatag ttaatactat atcgggtatt
      301 atatttctcc gaaataaact tcactttacc attcaataat acattcagta catcttgatc
      361 aaggtgggtg attttactta cccaatctgg gtcacgcaac atttcaatag cttttgacga
      421 aatgttattt agattccact catcaatatt tatcagtaaa aaaccagcat tgaaatatcc
      481 agaagctaac tgtggtgtag ttaataccga ggctcgattt tgccaccatt caatatctct
      541 ttcagctaca actgcggcaa tttcattagt agaaaattga taatctaaga gttctttaat
      601 actacccttg caagcaatat ctgcatcaag atatagtatt ttttcatgtt tatgatagaa
      661 ataatctgca attataaatc gaaaatatgt tgc
//
LOCUS       DAZSNG010000361          689 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZSNG010000361 DAZSNG010000000
VERSION     DAZSNG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.368"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            217..>689
                     /locus_tag="ACHW4Z_004515"
     CDS             217..>689
                     /locus_tag="ACHW4Z_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR8883806.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT          187 a          141 c          163 g          198 t
ORIGIN      
        1 gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat
       61 ttgcggcaat atagttattc catgaccgta caatcttgca ttcagtgtaa ataatcttgt
      121 ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta tccggcatat
      181 attgtttttg cttgctcatg gaatagggag gttgtcatgt atcaacgctg ttttgataat
      241 gccagtgaaa cgctgtttgt cgccggtaaa acgccacggc tttcacgttt tgcatttagc
      301 gatgatccaa aatgggagtc tggacatcac gttcatgaca atgaaaccga gctgatttac
      361 gtcaagaaag gggttgcaag gtttaccatc gattcttcgt tatatgtcgc acatgcggat
      421 gacattgtgg tgatagaacg cggcaggctg catgcggtgg cctctgacgt taacgatccg
      481 gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc agggggctga ggaaaatcag
      541 ttactgcaac cgcattcttg tccggtaatt gccgcggggc agggaaaaga agtcataaaa
      601 accttattta atgagctaag tgtgattttg ccgcaaagta aaaatagcca aacatcttcg
      661 ttatgggatg cattagccta tacattagc
//
LOCUS       DAZSNG010000362          685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZSNG010000362 DAZSNG010000000
VERSION     DAZSNG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.369"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            52..685
                     /locus_tag="ACHW4Z_004516"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHW4Z_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZSNG010000363          679 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZSNG010000363 DAZSNG010000000
VERSION     DAZSNG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.370"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..251)
                     /locus_tag="ACHW4Z_004517"
     CDS             complement(<1..251)
                     /locus_tag="ACHW4Z_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR8883807.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACHW4Z_004518"
     CDS             complement(272..>679)
                     /locus_tag="ACHW4Z_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8883808.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZSNG010000364          678 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZSNG010000364 DAZSNG010000000
VERSION     DAZSNG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.371"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..525
                     /locus_tag="ACHW4Z_004519"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACHW4Z_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>678
                     /locus_tag="ACHW4Z_004520"
     CDS             589..>678
                     /locus_tag="ACHW4Z_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_169729183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8883809.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVAT"
BASE COUNT          190 a          137 c          164 g          187 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaaca
//
LOCUS       DAZSNG010000365          671 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZSNG010000365 DAZSNG010000000
VERSION     DAZSNG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.372"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            53..>671
                     /gene="ospC1"
                     /locus_tag="ACHW4Z_004521"
     CDS             53..>671
                     /gene="ospC1"
                     /locus_tag="ACHW4Z_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR8883810.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDF"
BASE COUNT          257 a          113 c           96 g          205 t
ORIGIN      
        1 atcttgtagg gaaagatatt aattaaaact gttttcatat aaggttcatt ttatgaatat
       61 atcagaaaca ctgaactcag caaataccca atgcaatata gattctatgg ataacagatt
      121 acatacattg tttccaaaag tgacatcagt gcgaaacgct gcacaacaaa ctatgccaga
      181 tgaaaaaaat ttaaaagata gtgcaaatat tattaaaagt ttctttagga aaactatagc
      241 agcacagagt tatagtagaa tgttctctca aggctctaac tttaaatctt taaatatagc
      301 aattgatgca ccatcagacg ctaaagcctc atttaaggct attgagcacc ttgacagatt
      361 atcgaagcat tatatatctg aaataaggga aaaacttcat cctctttctg cagaggaact
      421 caatttgctt tcgctaatta ttaattctga tttaatcttc agacatcaaa gtaattctga
      481 tttgtctgat aaaattttaa acattaagtc attcaataaa attcagtctg aaggaatatg
      541 cacaaaacga aacacatacg ctgatgatat aaaaaaaata gctaatcatg actttgtgtt
      601 ttttggcgtt gaaatctcta accatcagaa aaaacacccc ctgaatacaa aacatcacac
      661 tgttgatttt g
//
LOCUS       DAZSNG010000366          656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZSNG010000366 DAZSNG010000000
VERSION     DAZSNG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.373"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..>656)
                     /locus_tag="ACHW4Z_004522"
     CDS             complement(151..>656)
                     /locus_tag="ACHW4Z_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8883811.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZSNG010000367          649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZSNG010000367 DAZSNG010000000
VERSION     DAZSNG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.374"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..355
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_004523"
                     /pseudo
     CDS             1..355
                     /gene="tnpA"
                     /locus_tag="ACHW4Z_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          199 a          158 c          124 g          168 t
ORIGIN      
        1 gtctttgtca ccagataccg acgccagatt tttgaccatg acgcgacaga aaaactacgc
       61 acttactttt caaatgtatg tgcttatttt gaagctgaac tggttgaaat ggatggcgaa
      121 ccagatcacg tccatctgtt aattaactat ccccccaaac tggcgatatc cagtctggta
      181 aacaaacagc ctcaaaggcg tatcgggtag gttactgcga cgggatcgac cagatattgc
      241 tgccaggtat taccgcaaag atgttttgtg gcgtcccggc tattttgcca gtagctgcgg
      301 aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcagat
      361 gaaaaaacga gccttatatc cccgccctga aggacgggct tttacggtgc actggataag
      421 cctggagatt atctacacct gcatggacat cattgtacta tgccccgtac atgctccatc
      481 acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca gtttgtctca
      541 tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc gtattaaata
      601 aaaccacctc aattacatta ataaatctaa ataaatatca tgcaaataa
//
LOCUS       DAZSNG010000368          638 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZSNG010000368 DAZSNG010000000
VERSION     DAZSNG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.375"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            90..155
                     /locus_tag="ACHW4Z_004524"
                     /pseudo
     CDS             90..155
                     /locus_tag="ACHW4Z_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            383..631
                     /locus_tag="ACHW4Z_004525"
     CDS             383..631
                     /locus_tag="ACHW4Z_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8883812.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          132 c          147 g          157 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
      541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
      601 ggagtctgct gacgagtggt ttgttcacta ataaacac
//
LOCUS       DAZSNG010000369          606 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZSNG010000369 DAZSNG010000000
VERSION     DAZSNG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.376"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(428..>606)
                     /locus_tag="ACHW4Z_004526"
     CDS             complement(428..>606)
                     /locus_tag="ACHW4Z_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR8883813.1"
                     /translation="SLKVDSLFISKDKRTYIKRKENKTNRTLIFTLSKINKTVQREID
                     EAIRDIISRHLSSS"
BASE COUNT          180 a           96 c          108 g          222 t
ORIGIN      
        1 gctaatatat tcttaaagct tttttgatac tgtgaacgaa gtctgttaat ggtggatttt
       61 ttgtttatca ccatgtaaaa aaaattgagt agtaaaagcg ttgactgcta atcctcgctg
      121 tttttattga aacgattaaa aatacgctga atattttcgg ccagtcactc atattcacat
      181 aaaattttga ccatcatgcc gcatccctta gttgcacaat tcattttcaa tgatatgaat
      241 attactaatt tcaccagata attttctcgt ggtcagcagg gtatgagttt tcaccgtaat
      301 tttaaggaaa cacagtgcgt ggggattgaa taacagatag ccgacggaat taccccaccg
      361 gctgaaagtc aagttcaacg aagattttta ttatctgaat tgggcagttt acatcagtgt
      421 tcgatgttta tgaagacgat agatggcgag aaattatatc ccgaatagct tcatctattt
      481 ctctctgaac tgttttgttt attttagata atgtaaatat tagagttctg tttgttttat
      541 tctcttttct ttttatgtaa gtccgtttgt ctttagaaat aaataacgag tcaactttta
      601 gcgaag
//
LOCUS       DAZSNG010000370          599 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZSNG010000370 DAZSNG010000000
VERSION     DAZSNG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.377"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>599
                     /gene="ospB"
                     /locus_tag="ACHW4Z_004527"
     CDS             <1..>599
                     /gene="ospB"
                     /locus_tag="ACHW4Z_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR8883814.1"
                     /translation="DYVFKNEKSKNDFLKMAESWLPQSEPIVINNDDDALNAAAYFSV
                     KKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELIDVQTIISRMKDCGILNVKD
                     IRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKESLLEQIKKHLENDESLSDG
                     LKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRS"
BASE COUNT          205 a           99 c          108 g          187 t
ORIGIN      
        1 tgattatgtt tttaaaaatg aaaaaagtaa aaatgatttt ctaaaaatgg ctgaatcatg
       61 gctaccacag agtgaaccaa tagtaataaa taatgatgat gacgcattga atgctgctgc
      121 ttatttttct gtaaaaaaag cgaaaataaa aacagtaaac gatactgatt ttaaagagta
      181 taataaggtt tatattcttg ggcacggtag tcctggttct catcaattag gccttggttc
      241 ggaacttatt gatgtacaaa caatcatttc aagaatgaaa gactgtggta ttctaaatgt
      301 gaaagatatc cgttttactt catgcggctc cgctgataaa gtggctccta aaaattttaa
      361 caatgcccct gctgaaagtc tttcttgtat ccttaactct ctgccttttt ttaaggaaaa
      421 agaatctttg ctagagcaga taaaaaaaca ccttgaaaac gatgagtcat tgagtgatgg
      481 tctaaaaata tccggctatc atggatatgg agttcactat ggtcaagagc tttttcccta
      541 ctcacattat cgttcaactt caattcctgc tgatccggag catacagtaa aaagaagct
//
LOCUS       DAZSNG010000371          590 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZSNG010000371 DAZSNG010000000
VERSION     DAZSNG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.378"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            487..>590
                     /locus_tag="ACHW4Z_004528"
     CDS             487..>590
                     /locus_tag="ACHW4Z_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883815.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQK"
BASE COUNT          217 a          105 c          121 g          147 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa
//
LOCUS       DAZSNG010000372          577 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZSNG010000372 DAZSNG010000000
VERSION     DAZSNG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.380"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..98
                     /locus_tag="ACHW4Z_004529"
     CDS             <1..98
                     /locus_tag="ACHW4Z_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8883816.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHW4Z_004530"
     CDS             156..512
                     /locus_tag="ACHW4Z_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883817.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAZSNG010000373          571 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZSNG010000373 DAZSNG010000000
VERSION     DAZSNG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.381"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..571
                     /gene="virF"
                     /locus_tag="ACHW4Z_004531"
     CDS             <1..571
                     /gene="virF"
                     /locus_tag="ACHW4Z_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR8883818.1"
                     /translation="RNLLLSIIRIMEPIYSFQHSYSEEKRGLNKKIFLLSEEEVSIDL
                     FKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFSDQIRKIVEKNIEKRW
                     RLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLLLNSQSYINDVSRLIG
                     ISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          205 a           81 c           91 g          194 t
ORIGIN      
        1 cagaaattta ttattaagca ttattagaat aatggaacca atttattcat ttcaacactc
       61 ctattctgag gagaaaaggg ggttaaacaa aaaaatattc ctcctctctg aggaggaggt
      121 ttctatcgat ttgttcaaat ctataaaaga gatgcctttc ggcaaaagaa agatctatag
      181 tttagcttgc cttttatcag ctgtttctga tgaggaagct ttatatactt cgatatcgat
      241 agcttcttct cttagttttt ctgatcagat aaggaagatt gttgaaaaaa acatcgagaa
      301 gagatggcgt ctttctgata tttcaaataa cttgaattta tcagaaatag ctgttagaaa
      361 acgattggag agtgaaaaat taacatttca acaaatcctt cttgatattc gcatgcatca
      421 tgcagcaaag cttttattga atagtcaaag ctatattaat gatgtatcaa gacttatcgg
      481 aatatcaagc ccatcttatt ttataaggaa atttaatgaa tattatggta taactccaaa
      541 gaaattttac ttatatcata aaaaatttta a
//
LOCUS       DAZSNG010000374          566 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZSNG010000374 DAZSNG010000000
VERSION     DAZSNG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.382"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHW4Z_004532"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHW4Z_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8883819.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACHW4Z_004533"
     CDS             complement(405..>566)
                     /locus_tag="ACHW4Z_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8883820.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAZSNG010000375          554 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZSNG010000375 DAZSNG010000000
VERSION     DAZSNG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.383"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>554)
                     /locus_tag="ACHW4Z_004534"
     CDS             complement(<1..>554)
                     /locus_tag="ACHW4Z_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR8883821.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          120 c          135 g          165 t
ORIGIN      
        1 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagactgc
       61 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
      121 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
      181 tctgactgat cgcttgggcg acggtgtttt gccattctaa agaacaggtg ctgaagtcaa
      241 atgtggctgt tttattatcc cgttgcgaca cgctacaggc taagcgacgt aattgggtca
      301 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
      361 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccaaggtaaa acagcatctg
      421 tatttttgtc agccgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      481 ataagtccgc taccttgaga gagtccgtta ccgtttggga aggttgcaaa gaattatctt
      541 tcgcttgctc caca
//
LOCUS       DAZSNG010000376          534 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZSNG010000376 DAZSNG010000000
VERSION     DAZSNG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.384"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          174 a           78 c          107 g          175 t
ORIGIN      
        1 tatatgaata tttcagagtt ggagttcgaa tgttgacacc tgagtatttc ctgctaaaaa
       61 tagccctgct ttacggatgg ttgtattgca ataacacgaa agtagtaaga gattaaaggt
      121 atctgtcagc gataactctg caagtgatga cattttgtaa accaaataat gtaaatcagc
      181 aatgtatctt ttcacttaat tataacaaat gtggtattca ctaagaatct gatacgagct
      241 tacatcacga ctattttagt ctcatcgcgg gatacaggga tgcaccgatg gatctcagca
      301 caccgtaatg caatacctga acaaatagaa cagtaaagtg gttcagtatt aatgaaagct
      361 cttattttcc gttgggaaaa cttaaaatag atgagaaggc tttttgattt cttatgtgat
      421 ctcatttcga gtattgagaa atttgtgatt tttaaatttt tcaataagcc ctgtaaggga
      481 ttggggtttt atgagaaata aagagacgta ttgtctttgt ttaaacggta cgta
//
LOCUS       DAZSNG010000377          529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZSNG010000377 DAZSNG010000000
VERSION     DAZSNG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.385"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            378..>529
                     /locus_tag="ACHW4Z_004535"
     CDS             378..>529
                     /locus_tag="ACHW4Z_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8883822.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          132 a          140 c          117 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctaggg tctctctagg gtctcctcta
       61 ggcattaccc ccagaagcta actctatggc cctctatggt cctctatggc cctgtacgtc
      121 attctttagg atgccctgta gtttcgtata gcctgatgcc tagagagacc ctagagagac
      181 cctagagaga ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca
      241 tcgtcatcat catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg
      301 gtctcccttc gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac
      361 aaaaaggaaa tcaagcgatg tctcaacaac cacaacaacc acaacaagaa atcagcatca
      421 ttaacctcga ccagcttgtg tccatgacct cagttgaaat tgctgagctg acaggcaagg
      481 agcaccgaaa tgtccttcgt gatattcgta agatgctcga agaacttaa
//
LOCUS       DAZSNG010000378          502 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZSNG010000378 DAZSNG010000000
VERSION     DAZSNG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.386"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            133..502
                     /locus_tag="ACHW4Z_004536"
                     /pseudo
     CDS             133..502
                     /locus_tag="ACHW4Z_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a          107 c          168 g          108 t
ORIGIN      
        1 tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtgt ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt ac
//
LOCUS       DAZSNG010000379          498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZSNG010000379 DAZSNG010000000
VERSION     DAZSNG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.387"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..498
                     /locus_tag="ACHW4Z_004537"
                     /pseudo
     CDS             1..498
                     /locus_tag="ACHW4Z_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          121 a          142 c          144 g           91 t
ORIGIN      
        1 ggcgcagtaa acagcaaaat cagcagacgt cttctgaacg tgaactggag atgtctaccg
       61 agattgcacg tctcaaacgc cagctggcag aacgggatga agagctggct atcctccaaa
      121 aggccgcgac atacttcgcg aagcgcctga aatgaagtat gtctttattg aaaaacatca
      181 ggctgagttc agcatcaaag caatgtgccg cgtgctccgg gtggcccgca gcggctggta
      241 tacgtggtgt cagcggcgga caaggataag cacgcgtcag cagttccgcc aacactgcga
      301 cagcgttgtc ctcgcggctt ttacccggtc aaaacagcgt tacggtgccc cacgcctgac
      361 ggatgaactg cgtgctcagg gttacccctt taacgtaaaa accgtggcgg caagcctgcg
      421 ccgtcaggga ctgagggcaa aggcctcccg gaagttcagc ccggtcagct accgcgcaca
      481 cggcctgcct gtgtcaga
//
LOCUS       DAZSNG010000380          487 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZSNG010000380 DAZSNG010000000
VERSION     DAZSNG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.388"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>487
                     /locus_tag="ACHW4Z_004538"
     CDS             <1..>487
                     /locus_tag="ACHW4Z_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR8883823.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KR"
BASE COUNT           97 a          163 c          131 g           96 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgg
//
LOCUS       DAZSNG010000381          484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZSNG010000381 DAZSNG010000000
VERSION     DAZSNG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.389"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..484
                     /locus_tag="ACHW4Z_004539"
                     /pseudo
     CDS             1..484
                     /locus_tag="ACHW4Z_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaa
//
LOCUS       DAZSNG010000382          473 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZSNG010000382 DAZSNG010000000
VERSION     DAZSNG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.390"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..473
                     /locus_tag="ACHW4Z_004540"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHW4Z_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZSNG010000383          472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZSNG010000383 DAZSNG010000000
VERSION     DAZSNG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.391"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..>472
                     /locus_tag="ACHW4Z_004541"
     CDS             3..>472
                     /locus_tag="ACHW4Z_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR8883824.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZSNG010000384          471 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZSNG010000384 DAZSNG010000000
VERSION     DAZSNG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.392"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..361
                     /locus_tag="ACHW4Z_004542"
     CDS             <1..361
                     /locus_tag="ACHW4Z_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8883825.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZSNG010000385          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZSNG010000385 DAZSNG010000000
VERSION     DAZSNG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.393"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>459)
                     /locus_tag="ACHW4Z_004543"
     CDS             complement(<1..>459)
                     /locus_tag="ACHW4Z_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR8883826.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZSNG010000386          448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZSNG010000386 DAZSNG010000000
VERSION     DAZSNG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.394"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..382)
                     /locus_tag="ACHW4Z_004544"
     CDS             complement(<1..382)
                     /locus_tag="ACHW4Z_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883827.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DAZSNG010000387          443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZSNG010000387 DAZSNG010000000
VERSION     DAZSNG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.395"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            213..>443
                     /locus_tag="ACHW4Z_004545"
     CDS             213..>443
                     /locus_tag="ACHW4Z_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8883828.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZSNG010000388          434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZSNG010000388 DAZSNG010000000
VERSION     DAZSNG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.396"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            35..434
                     /locus_tag="ACHW4Z_004546"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHW4Z_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZSNG010000389          417 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZSNG010000389 DAZSNG010000000
VERSION     DAZSNG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.397"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..269
                     /locus_tag="ACHW4Z_004547"
     CDS             <1..269
                     /locus_tag="ACHW4Z_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8883829.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>417
                     /gene="tnpB"
                     /locus_tag="ACHW4Z_004548"
     CDS             266..>417
                     /gene="tnpB"
                     /locus_tag="ACHW4Z_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8883830.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSG"
BASE COUNT          100 a          112 c          119 g           86 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtgg
//
LOCUS       DAZSNG010000390          416 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZSNG010000390 DAZSNG010000000
VERSION     DAZSNG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.398"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACHW4Z_004549"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACHW4Z_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR8883831.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZSNG010000391          398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZSNG010000391 DAZSNG010000000
VERSION     DAZSNG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.399"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            321..>398
                     /locus_tag="ACHW4Z_004550"
     CDS             321..>398
                     /locus_tag="ACHW4Z_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8883832.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          127 a           72 c           79 g          120 t
ORIGIN      
        1 aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga gtttaatcac
       61 acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat atatatcttg
      121 cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg caaaacaggg
      181 catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa gaatcatggg
      241 cgctcaacaa aaagagggta atgctgccaa cttactgatt tagtgtatga tggtgatttt
      301 aaggtgcttg cgtagcttcc atttccatca gatgtccttc ctgctccgct actgaaggcg
      361 tggtgcgtaa cggcaaaagc actgccggac atcagcgc
//
LOCUS       DAZSNG010000392          391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZSNG010000392 DAZSNG010000000
VERSION     DAZSNG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.400"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(42..>391)
                     /locus_tag="ACHW4Z_004551"
     CDS             complement(42..>391)
                     /locus_tag="ACHW4Z_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8883833.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          121 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt t
//
LOCUS       DAZSNG010000393          383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZSNG010000393 DAZSNG010000000
VERSION     DAZSNG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.401"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..87
                     /locus_tag="ACHW4Z_004552"
     CDS             <1..87
                     /locus_tag="ACHW4Z_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8883834.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..>383
                     /locus_tag="ACHW4Z_004553"
     CDS             102..>383
                     /locus_tag="ACHW4Z_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8883835.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           93 a           79 c          118 g           93 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gac
//
LOCUS       DAZSNG010000394          379 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZSNG010000394 DAZSNG010000000
VERSION     DAZSNG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.402"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(273..>379)
                     /locus_tag="ACHW4Z_004554"
     CDS             complement(273..>379)
                     /locus_tag="ACHW4Z_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR8883836.1"
                     /translation="SGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          109 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactat
//
LOCUS       DAZSNG010000395          375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZSNG010000395 DAZSNG010000000
VERSION     DAZSNG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.403"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>375
                     /locus_tag="ACHW4Z_004555"
     CDS             <1..>375
                     /locus_tag="ACHW4Z_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8883837.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZSNG010000396          372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZSNG010000396 DAZSNG010000000
VERSION     DAZSNG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.404"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..311)
                     /locus_tag="ACHW4Z_004556"
     CDS             complement(<1..311)
                     /locus_tag="ACHW4Z_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8883838.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZSNG010000397          359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZSNG010000397 DAZSNG010000000
VERSION     DAZSNG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.denovo.405"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..226
                     /locus_tag="ACHW4Z_004557"
     CDS             <1..226
                     /locus_tag="ACHW4Z_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8883839.1"
                     /translation="SPYLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYG
                     TPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            148..>359
                     /locus_tag="ACHW4Z_004558"
     CDS             148..>359
                     /locus_tag="ACHW4Z_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8883840.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGF"
BASE COUNT          115 a           79 c           93 g           72 t
ORIGIN      
        1 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
       61 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      121 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      181 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
      241 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
      301 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctg
//
LOCUS       DAZSNG010000398        33529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.034, whole genome shotgun sequence.
ACCESSION   DAZSNG010000398 DAZSNG010000000
VERSION     DAZSNG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33529
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.034"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            558..797
                     /locus_tag="ACHW4Z_004559"
     CDS             558..797
                     /locus_tag="ACHW4Z_004559"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR8883841.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHW4Z_004560"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHW4Z_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHW4Z_004561"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHW4Z_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8883842.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHW4Z_004562"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHW4Z_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8883843.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHW4Z_004563"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHW4Z_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            4308..4805
                     /locus_tag="ACHW4Z_004564"
     CDS             4308..4805
                     /locus_tag="ACHW4Z_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883844.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4866..5237
                     /locus_tag="ACHW4Z_004565"
     CDS             4866..5237
                     /locus_tag="ACHW4Z_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883845.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5647..6024
                     /locus_tag="ACHW4Z_004566"
     CDS             5647..6024
                     /locus_tag="ACHW4Z_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883846.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            6047..6199
                     /locus_tag="ACHW4Z_004567"
                     /pseudo
     CDS             6047..6199
                     /locus_tag="ACHW4Z_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(6255..7871)
                     /locus_tag="ACHW4Z_004568"
     CDS             complement(6255..7871)
                     /locus_tag="ACHW4Z_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR8883847.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7872..9398)
                     /locus_tag="ACHW4Z_004569"
     CDS             complement(7872..9398)
                     /locus_tag="ACHW4Z_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR8883848.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(9401..11068)
                     /locus_tag="ACHW4Z_004570"
     CDS             complement(9401..11068)
                     /locus_tag="ACHW4Z_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8883849.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(11065..13173)
                     /locus_tag="ACHW4Z_004571"
     CDS             complement(11065..13173)
                     /locus_tag="ACHW4Z_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR8883850.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(13160..13981)
                     /locus_tag="ACHW4Z_004572"
     CDS             complement(13160..13981)
                     /locus_tag="ACHW4Z_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR8883851.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(14143..15972)
                     /gene="glmS"
                     /locus_tag="ACHW4Z_004573"
     CDS             complement(14143..15972)
                     /gene="glmS"
                     /locus_tag="ACHW4Z_004573"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR8883852.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(16134..17504)
                     /gene="glmU"
                     /locus_tag="ACHW4Z_004574"
     CDS             complement(16134..17504)
                     /gene="glmU"
                     /locus_tag="ACHW4Z_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR8883853.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17855..18274)
                     /locus_tag="ACHW4Z_004575"
     CDS             complement(17855..18274)
                     /locus_tag="ACHW4Z_004575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR8883854.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(18295..19677)
                     /gene="atpD"
                     /locus_tag="ACHW4Z_004576"
     CDS             complement(18295..19677)
                     /gene="atpD"
                     /locus_tag="ACHW4Z_004576"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR8883855.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(19704..20567)
                     /gene="atpG"
                     /locus_tag="ACHW4Z_004577"
     CDS             complement(19704..20567)
                     /gene="atpG"
                     /locus_tag="ACHW4Z_004577"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR8883856.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(20618..22159)
                     /gene="atpA"
                     /locus_tag="ACHW4Z_004578"
     CDS             complement(20618..22159)
                     /gene="atpA"
                     /locus_tag="ACHW4Z_004578"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR8883857.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(22172..22705)
                     /gene="atpH"
                     /locus_tag="ACHW4Z_004579"
     CDS             complement(22172..22705)
                     /gene="atpH"
                     /locus_tag="ACHW4Z_004579"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR8883858.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(22720..23190)
                     /gene="atpF"
                     /locus_tag="ACHW4Z_004580"
     CDS             complement(22720..23190)
                     /gene="atpF"
                     /locus_tag="ACHW4Z_004580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR8883859.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(23252..23491)
                     /gene="atpE"
                     /locus_tag="ACHW4Z_004581"
     CDS             complement(23252..23491)
                     /gene="atpE"
                     /locus_tag="ACHW4Z_004581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR8883860.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(23538..24353)
                     /gene="atpB"
                     /locus_tag="ACHW4Z_004582"
     CDS             complement(23538..24353)
                     /gene="atpB"
                     /locus_tag="ACHW4Z_004582"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR8883861.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(24362..24742)
                     /gene="atpI"
                     /locus_tag="ACHW4Z_004583"
     CDS             complement(24362..24742)
                     /gene="atpI"
                     /locus_tag="ACHW4Z_004583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR8883862.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(25359..25982)
                     /gene="rsmG"
                     /locus_tag="ACHW4Z_004584"
     CDS             complement(25359..25982)
                     /gene="rsmG"
                     /locus_tag="ACHW4Z_004584"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR8883863.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(26046..27935)
                     /gene="mnmG"
                     /locus_tag="ACHW4Z_004585"
     CDS             complement(26046..27935)
                     /gene="mnmG"
                     /locus_tag="ACHW4Z_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR8883864.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(28314..28757)
                     /gene="mioC"
                     /locus_tag="ACHW4Z_004586"
     CDS             complement(28314..28757)
                     /gene="mioC"
                     /locus_tag="ACHW4Z_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR8883865.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(28847..29305)
                     /gene="asnC"
                     /locus_tag="ACHW4Z_004587"
     CDS             complement(28847..29305)
                     /gene="asnC"
                     /locus_tag="ACHW4Z_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR8883866.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            29457..30449
                     /gene="asnA"
                     /locus_tag="ACHW4Z_004588"
     CDS             29457..30449
                     /gene="asnA"
                     /locus_tag="ACHW4Z_004588"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR8883867.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(30454..31905)
                     /gene="viaA"
                     /locus_tag="ACHW4Z_004589"
     CDS             complement(30454..31905)
                     /gene="viaA"
                     /locus_tag="ACHW4Z_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR8883868.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31899..33395)
                     /gene="ravA"
                     /locus_tag="ACHW4Z_004590"
     CDS             complement(31899..33395)
                     /gene="ravA"
                     /locus_tag="ACHW4Z_004590"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR8883869.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
BASE COUNT         8541 a         8466 c         7826 g         8696 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta gagagctggg gaagactatg cgcgatctgt
     4321 tgaaggtggt tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc
     4381 caattgtttt agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca
     4441 tttctccaag caactacgac aactccataa gcaattacga caatagtcca tcaaattacg
     4501 acaactctga gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa
     4561 atcgtaggct tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg
     4621 ccaacaatgg gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa
     4681 aaggggggcg cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca
     4741 taaatggcca attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca
     4801 actagcctgc tctctaataa aatgttaggc ctcaacatct agtcgcaagc tgaggggaac
     4861 cactagtgtc atacgaacct ccaagagacg gttacacaaa cgggtacatt gttgatgtca
     4921 tgtatgacaa tcgcccaagt aagtatccag ctgtgttcag aacgtacgtc cgaattccag
     4981 actatgtcga actagataac gcggaggagc tggccgacgc aatcattcga gaggttgagt
     5041 taaaggaggc atggctcaca agggtcttgg ggaactcagc cgatcctgac gcttctgtgg
     5101 gcaagtggga ccggcgccta gcgcagtgcg gtggtattga tgagatgtca agtggggtgc
     5161 aggcaacata cttcagtcca aacggtcagc tggtcggctt agtggcatac attgatgccg
     5221 aatggatgga tgaatagctc tgacgttacg attcaggtga gacaaccaga tgggcattgc
     5281 gtacaagctc agttgccacc gatgtggcta cgagtctgat cttctttacc taggccaagg
     5341 tatggcgatg cttcctgagc aaatagtcgg aacgtgcaca tgctgcgaca atctaacgac
     5401 gatttctata aatgaaagct acggagtatg ctcactttgt ggggctaagg atcgtgtgat
     5461 atttacaagc tatcagcatg aggcaaggcc aagaaatccc tgctatctag agagaagttt
     5521 caaatatcaa tgcccaagat gccatgcctt cagtatggag cctccggatc taccggagat
     5581 gctttgggac taatgctccg ctttgcctcg ggtatccgtt cgacctatgt ttaagaggaa
     5641 acgcacgtgt ctgccgccaa agctcttgcc tatgcacttt gtgacaagtt ggaaagcaaa
     5701 aaagttgatg aagtcgtacg aaatcttgtt tctagcggct ggaatattag ggaagtggcc
     5761 tgggaagctt cgtcctttga tcatgaaatg ccctactttg acacggggcg ggacttcgat
     5821 attggatttc ctgacacgct gtcatccaag attgagtttt tgaccgcgaa tggatcaatc
     5881 gtccaagcac gtgctcgcct gcacttcaag caggctctaa tttttgccaa ggcgtccaaa
     5941 catgtcgccg cgctcaagaa ctcattggat tactactatg gatcgtctgt aatcccccct
     6001 caaaaccacg gaggctattt gtagaaaatt ccattaatat gaactcaagg tgttccctac
     6061 ggacgaatca gcgatgaagg tggtgtacct ggctatccag gcggcgacca agaaatggac
     6121 gatgccgatc cgcaactgga agccggccat gaaccgtttt atgatcgaat ttggtgaccg
     6181 tttaaatggc cacctttaac tgaccggcaa aagcacttac acaaaagggt ttacaggctc
     6241 tttcagtcat tcctttaatg cgtaaattgc tctctaacca ctctctcagc ccacccttcc
     6301 atatcctctc tcgatacttg cagctccccc aacccctttg gatgattcac ccctctatgc
     6361 ccgtcttgtc catataattg atcaaacaaa ctatttggcc aattcaatga gctcttcacc
     6421 actccacgcc gtatcttttc aaaatcattc cgccatgttt cgctatcaac gccactcaac
     6481 actttggtag aaagcatttt caccccatcc gatgcatcca cctctagcaa tacaaattca
     6541 ctgccattac gttgcacccc aacggcttta atcactcttg gtgagccatc tttacacaga
     6601 tgtaatcggc tacgcccaac ttttggcaaa accaaggttt cttcaaaaag cacccgacat
     6661 gcaaatttag tttttagaat tgaaagtagc tcatcaaaag cagcaaatcg attagcaaaa
     6721 atagagttgt aattggttgc atcctgcttc ccacccacat ctgccgctgc taggacacca
     6781 cctaagtgtg gctcatcagt actaaccaaa ttactatttt cacttgttgt tgcttcttct
     6841 ttttctaaaa tagtttggct actttttctt gccctgcgag atttttcaat tcgactaggt
     6901 ctactaaaac ttatccatgt cggctcagcc tctatgactc gtgtgtctgt gtctatattg
     6961 gctgtttcct catcgtccaa ctgtagatgc tcatcatcaa caactgactc tgttgaaacc
     7021 gctatgtgct gcgtactacc atcacctgcc tccttttcct gaaaagaggc atggctaata
     7081 gctgttgtgc taggaagcat agcgttgatt tctaaaccta ctatttcctc aactaaataa
     7141 tccttatcct cgttagaaga acgtcctttt acatgtaatt tccaaccctg catgggcgga
     7201 gggtcaaagc taaagcacca gctttcaacg ccgttcttaa tttctctatt ttgttgatag
     7261 tgcctaaaaa tactttcata cgaatccata acatcttgat ccgaaaacaa ccaaaccaaa
     7321 agctgcacta cggccgacag ctctaacgcc cctttaggaa acgatgaact gggtaagatc
     7381 cttatctcta aatgatctcg ctcaacctca tactgaacgt cgaactcttg ctgtaaagcg
     7441 gtactgctca aacagcttcg acagaaatag gagttaatta aaaataatga ccgagctagc
     7501 tctaactgtg ggatgtgata acgagtcttg ctatatggtg tttcagcttc gtaaataaat
     7561 gaactctgcg ctccatcctt gttgcgtacg gaagcaaagg acgagagatt aggaaaatcg
     7621 attattttgg ctcgttgaag atccgatgct tcaaactcaa tcacacgatc cgccttattt
     7681 atttgctttg ttgtactgtt aaccacctta gctcgactta ataaaggcaa atgagtaaat
     7741 cgagtccatt tccgttcttg cattggatta aaccaaacaa aaatacgcca ctccttatga
     7801 cccgagttac ggaataaatg accaatatgt acaacctgca cattgtcatt aaatgtagct
     7861 agcctaacca ctcactcttc cccataaacc atttccaaga atctttgtgc ttcctcagtt
     7921 atccgctctt tagataacgt tgcacttctt aataatcgcc agcgcctaag ctcaacatct
     7981 tcctgtttaa gcttaataaa agcttggcta atccgtctaa tttgataatc ttccacactc
     8041 tctgagtaac gctttaagca aagcgctacc aaaggcagtt tctgtagatt ttttgcaaga
     8101 gaggttccgt taggagtttg ctttaacagc cagctcgatg tcgctcttgg gtgatcaagg
     8161 ctactatcta gacgctttat gattcttaat agttgtcgta cagcaattcg atctctttgg
     8221 ttccaatcaa ctctaggggc gggtgccaga cgctcttgtt gatgctgttg attccagtga
     8281 actagccaat ccctgtcatg tcggtaaagc caagcgtata gcaccccacc ctctaaagac
     8341 tgtcttgccg ccttaattcc ttggtattta tgcactagtt gctgccagtc tttatgctta
     8401 acagataaat cttcagagtt aggttgcaca gactggctaa caggtcttgt cgttatagag
     8461 tgctcagtaa gagcacttgc ctgctgtagc gcttctataa ccgttagttt tggcaataag
     8521 gcttgccaca caatactatg ctgtaaataa ctaaaggctt ttctatgctt acggaatata
     8581 cttttcagcc aacacgtgtc cttgttctct gccaacttta aatccagttt ctctagtgcc
     8641 tcatcactaa aagtctgcct cactctctcc gccaccaagt catgacgaat gtgcttgctt
     8701 ttggttagcc ctagatcctg cgctaagcgc tgataaaaca gcgtccactg ctcaaggctt
     8761 ggggaaagct cttgcgctcg tggagcatct aacagagggg ctatataagc agctagtgct
     8821 gttaattgag atagggagtc tttggggtag tctgaaagca gctcagtatg acccaaagcc
     8881 caaaattgat gtcggtgatc atctacagct ctatcaaaga agactaaagc accgtgtttt
     8941 ggacaatatg gcaaagcggg caaataccaa tctcgttgcc aaaaggcttc cccataccta
     9001 tttagctgaa gagcaacgca atcagggcag tatctaaagc ggttatcgct cttaactcta
     9061 gaagcagcga ctcctagcat taaatgcacc gcaccttgcg cttggtactc cattaaccga
     9121 atagcttcgt ctcggcgctc cttgcctaca aacggagcat ataaagggaa taaggtatgc
     9181 tcataaataa gctgctgaac agcgtaacgt cctgtttgat gtagatgtct tgctatcaca
     9241 cctaaatgcg agggcagacc taaggtagcg accaccttgc ggttgccata cacctcatcc
     9301 aacagctgct taggactaac aatcccttga taaacgcctg cccgtgcaat agtgctataa
     9361 atcagctcat tcgagtacgg aacaggaaag tttctcatgg ctaacctgct tgtttaaata
     9421 agctcgccat atccactatt acccctttcc cttttaaccg ttcatgcatg gttttttcag
     9481 gttggcgttg tgaatagata taacgtaaat ccgtatcagg caagctatcc cagtcgctgg
     9541 gcttgactac ctttatagcc gaaacctttt tactcttgga gggcttttct agttctgaca
     9601 ctaccgtttc gccttccatc aaccactgca aaacaagagg cagtaacttt tgtcttgtca
     9661 tcgttggatt ctggctaaac gcttttttaa tagtgggaat taacaggctt gaatcgtaat
     9721 cctctttcag catcagatat aaatgacgct gatcttcggt atctaactct tgaagggctt
     9781 tttcttctgg tgtttgttct tgtatcgctg cgatatctag ctgaagttgg attaaccgtt
     9841 tatcaatctc gggaacgact agatcagaat aacgagcaat gcgttctggg atacccgagc
     9901 gtaatgcctc tagcatgggg tgcacaggct ttaactcatc ttgatacact tgccgcaata
     9961 aaccagcggt aatacgctca ttgcctaaag ctagcgcacg gagctgagcg agtacaaaaa
    10021 gttttactac aatgtccatc actccttggc ttagctcata ccacacatca cggacctcat
    10081 ccgataacag cgcatctttg cgttgtaaaa gctgtaattg ccaaagatta tccgtaaaag
    10141 cgatccactc ttgattgggc tttccacgtt gcgtttgttg tataggatcc cagaatatag
    10201 ctccaaaccc tgcccctcta cgtgcagacc gcaaatcagc ctcaaaaatc tctcgtgctt
    10261 taggggtacc aatcaacatc actggtacgc caataatatt caccatcgtc acaaaaaagt
    10321 tcagcatctc ttgagatcca cccgaacgag agcggcttaa atgctgaatt tcatcaataa
    10381 ccaacaaccc taaagcatgt gcattggcta tttgcgacat caaagccaac atggtttcta
    10441 taccatgacg ttttaagcca taacgacgct catagttcga gcccaaggct cgatccaacg
    10501 ctctgaaaaa attcaagcag atttctttta gcgaaccatt atgcgagcag tctattttca
    10561 aatacaccac ctgctctaca ttgagttcac gatggtaaat cacctgagga tacgtggcta
    10621 gaatacgatg aagagaggtc gtcttcccac taccagaaca accaattaac aataagcttt
    10681 gtgccgtaga tcgtgcctcc tcaaagcgaa atgtctccaa ctctcccgtt tgaacacgct
    10741 cataaccatt ttgtaaatgc ttttgtaaat ctcctgtttt aggatttctg cctacgtagc
    10801 cacctcgaat catgaccgaa atacgctcac ttagtagcaa atgcgtacct aatggctgaa
    10861 aatagtcatc tggaatacga caaatggtat gagctctgat aacacgggac ttttgcaagt
    10921 cagaggaagt aagctgtaaa gaggatttca gtgatgcagc actattcact gactcttgta
    10981 atggtggtaa ggcctcgata aaaggattat cacgataagc ctctaccccc gtatcacgat
    11041 aaactgcttg aatccgggta gcactcatga ctcatccttt tctggtggat cctgaaataa
    11101 ttcaggcacg tatgtgggta ggctgtaatc ttcttgatca tccgcttcca ctgcgttgaa
    11161 aggaataact ttagcctcat cacctgaaga gcttggcttc aaatgctccg cacgtttttt
    11221 acgctccgag gtcacggctt cttttttatt agttttaatc tgcttaatgc gtgttgattt
    11281 gggctcagta gtactgggcg ttaacttatt cgctttctga atggtttgct gaataaacgc
    11341 ctcaagctcc ctgcgtttag ttaactcatc ctgcttcgca ttggctttat tgtgtttttc
    11401 ttgtgcttgt atatcccaaa cctcccaaaa tgagagacct ttaaactgcc gactacgttc
    11461 cgtcagatta cagcgccaaa atacacggct gccaacttgc ggaaacaaat aaatcgtatc
    11521 aaccagcact gggtcataag ccgcttctaa atgttgaggt ctagctatat cagtgctccg
    11581 ctgcaaccaa ccctcacgca gaatctctga ccccgagtaa tacaaacccc acaaattaac
    11641 gccaaatgaa gaaatagaga cctttcggcg aggcagtaac gctactcgca actgctcttg
    11701 ctccacagcc cttaaactac ctgtacgatg ctgcataccc cattgccata gctggacagg
    11761 aatagacggt aaatctgtag gaaaatcagc atctcgatcg tatttatcca tcaccagatg
    11821 gttatttctg aataagatcg tacgcaaaat aatttgtgtg aactcaaata ccgacagaga
    11881 tgcatctaac ctatagtctg tttcaccatg gcttttgatc cgactgccct ctacaatgcc
    11941 aggtgcaaag gacttaaact cggcttgtag tgttctaaaa gtgctttcca ctatgccttt
    12001 agcatcgcca cgtctaggtg gagcactttc cactcgcaca ttaaaactag aaactaaggc
    12061 ttcgacctga tgactcatta attcgccacg gtccgctagc aacacatctg gcaaacctac
    12121 acacggccag tctgagctac taatctcaat atcatgctgg gcacaaatgg ccgttttgtc
    12181 agagcaagca tttacaaaag cctgcatcgc caccacataa gacggatttt caaagccgat
    12241 ataaaagccc gtgatcatcc gactaaacac atcaatcaca atgtaaagcg ttggtcttcc
    12301 tatgattttt tggcgatcat gatgatccac taaataaata tcagcaatcg tggcatcaat
    12361 ctcataacga ctcccagggc ctaacgcctg agaagtggct gtactactta agggtcgtac
    12421 gtctttttta tatacccctg ctttaactct agactttaag cgctgagctt tagggtattc
    12481 tcgatcataa aaataacgaa actgacgtag tgttgggtaa tcctcttggg gaatgcgagg
    12541 aaaatactga gcaaacaagt ccacaaatcg tctataggca acggtggtct ttgtaccttt
    12601 ttgatttaac aggtgctttt ctatggtcaa cctaaaaagg cgttcaatct cgggcgttac
    12661 cttggttcct tcacccttac catattctct ggctcggcca atctttgctg ttcctgtcgc
    12721 tgaacgtctt tcccctggtg caccgctgtt tttgtagtca ggaattaatg cattaggcgt
    12781 ttgaccacgc tgccagtaac ggcgtaacaa cttataaact gtagccttag taaccttatg
    12841 ttcttggacc acatgctcaa cgagttcgct tctgacttta gggtcgaaac gatccttact
    12901 attaataata ggaagaattt ttcgataatc ctcctcgcgc ttctggaagc tgacagaatc
    12961 tacagacggc tcttctaaat caagatgtac ataagggtca tcagttctaa ctaagcggcc
    13021 ttcatctaaa tactgcatta gcaacaactc agccctagct tgtggaactc ctttatcagc
    13081 gcttatttgc atccagacaa cttggccatc ctctatagcc aaaatgcgat acggatcatt
    13141 atcaaatagc acaacctcat taatttgcca catagcgcaa ctcctccata ttcactactt
    13201 ggctaataca gagatctgca cacttatttg ccctgaaaga cttataaata ttgaacttaa
    13261 taaaaccatt tgcggttaag gctcgtatct cactcaatgt tttgcctaac tccaaatcat
    13321 aagcaatatc aacctgctta cagacattga taatgttttc atctcctttt tcttgcagga
    13381 tatgggccaa tggggatagt tgtgctaaaa gctccgcaga aacttcttct gttttcactg
    13441 aataaagcca ttcaatattt tcttttacta cgggatttat ttctttatca gtaaaaatga
    13501 accaaggaat ttgcttttgc tgccaatagc gacgctctag ttctagtttt tctaaggtac
    13561 gctcgtcttg taaggctgct gcaggtttga cttgaatagc aaactgctca aaaggaccat
    13621 ctttgcagtc cactaaaaaa tcagtagaca taacctgatc tacaccacga ataacaggat
    13681 gcttaatacc actatctatt gcaatctgcc tggtatcact aggtaataag gggaactgct
    13741 cgcgtatatc tagcacgctg ctctcccact caagactgag aaaaacagca agctctaagt
    13801 cagatagcaa atgatggact cgtcccgtct tatgagaata aatacggtgg gaacgacctg
    13861 aagaaggaac ttcttgtact gttagccatg gaatatagtc tttaccatgc ccttggccac
    13921 gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca
    13981 taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat
    14041 tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt
    14101 tattgtccgc ccacagcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga
    14161 ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
    14221 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
    14281 ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata
    14341 caattgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc
    14401 gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg
    14461 tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa
    14521 tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt
    14581 gtcagagaaa tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat
    14641 acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc
    14701 tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc
    14761 tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc
    14821 gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc
    14881 tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat
    14941 catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac
    15001 gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata
    15061 agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt
    15121 cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt
    15181 tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc
    15241 gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt
    15301 atcgaagatg tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat
    15361 aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat
    15421 acccaggcca atcaccagcg gactaccaga acgtgccgct agcagggtat ccgggtgacg
    15481 ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc
    15541 ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt
    15601 gtcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg
    15661 gttttcgatg atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt
    15721 agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg
    15781 cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg
    15841 agtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc
    15901 cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac
    15961 aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt
    16021 tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg
    16081 gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt
    16141 ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat
    16201 gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct
    16261 actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg
    16321 gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg
    16381 ttatcgccaa tttccgcatc gcccaggtaa gtcagatgcc cggctttcgt gcctttaccc
    16441 agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca
    16501 ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc
    16561 acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa
    16621 ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta
    16681 tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg
    16741 cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc
    16801 agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga
    16861 acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat
    16921 tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc tgcgccgttg
    16981 gcaatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg
    17041 tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga
    17101 tcatccagtt tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc
    17161 tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca
    17221 tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga
    17281 agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg
    17341 tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc
    17401 atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct
    17461 ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta
    17521 atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa
    17581 tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg
    17641 accatcgtca cgccttttat ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg
    17701 tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg tgttttatat
    17761 cagcaggatc tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa gccagcctgt
    17821 ttccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg gtcaactcga
    17881 taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg taatctacgt
    17941 cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg gctcgcgctt
    18001 cgtcgagatc ctggccgcga attgcggtgt cggccagaac ggtcacgttg ccaggctgca
    18061 cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt ttcacgatgc
    18121 gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag atccccagtt
    18181 caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt tgttgctctg
    18241 cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa ggcgttaaag
    18301 ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg cctgctccgg
    18361 caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt ctttcaggga
    18421 gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct gggacaggaa
    18481 gcgctggatc ttacgagcac gcgctaccac cagtttgtct tcttcagaca gttcatccat
    18541 acccaggatg gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg actgaacgcc
    18601 acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac ggctggtgga
    18661 gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc tcagtaccac
    18721 ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt catccgcagg
    18781 tacgtatacc gcctgtacgg aggtgataga accagttttg gtggaggtga tacgttcctg
    18841 cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag gcatacggcc
    18901 cagcagtgcg gatacttccg taccggccag ggtgtaacga tagatgttgt caacgaacag
    18961 cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg tcagagcaac
    19021 gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata ctttgtcgat
    19081 aacgttggag tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac gttcacctac
    19141 gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaatga gctccatcat
    19201 gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc ccttagcgaa
    19261 cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag agtttgacag
    19321 ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc
    19381 tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg ctttacctac
    19441 cgggacttcg atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga
    19501 ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa cttccagcac
    19561 cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta cggcatcctg
    19621 agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag tagccatctt
    19681 aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt
    19741 tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc tttaatcagg
    19801 ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg ctcgctggcc
    19861 aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag cagggtatcc
    19921 agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt cagatcatca
    19981 tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga catggtgtta
    20041 ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac
    20101 atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat gccggtgacc
    20161 tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga gccgatcatt
    20221 gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag cagttttttg
    20281 aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac caggtagccc
    20341 acgcgtttaa cgtcgcggtc ttccaggtaa gggtgcttat attccagatt accgtgtgca
    20401 aggtgaccaa tcactttgcg catggtttct gcataaggac ggctggccgc catgcgatcc
    20461 tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat cttttgcgtg
    20521 ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag cttctcctca
    20581 atgccttgcg gcctgcccta aggcaagcct ccagacgtta ccaggattgg gttgctttga
    20641 aggaatcgag gatgcctttc agcttgcctt cgatttcgtc gttgtagcca ccggtctggt
    20701 tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga gcggcttcga
    20761 agctgccaat tttcgacagt tcaacatccg ccaggtaacc acgttctgct gcgaacagaa
    20821 ccagagactg ctgcgcaacg gacatcggcg catactgttt ctgtttcagc agttcggtca
    20881 ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag
    20941 agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg gacagttttt
    21001 tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc gggttaaccg
    21061 caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca tcggtaatgg
    21121 agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttcg ataatcggca
    21181 gtgcggtcag agaaccggtt ttccctttca cttcaccttt ggtgaaggct tcaacgtatt
    21241 cggcgttaac acgtgcagca cgctccagca gacgagagtg gaggtagaaa acgtcgcccg
    21301 ggaatgcttc acgtcctggc ggacgacgga gcagcaggga gatctgacgg taagcaacag
    21361 cctgtttaga caggtcatcg taaatgatca gcgcatcttc accgcggtca cggaagtatt
    21421 cgcccattgc gcaaccggca tacggtgcca ggtattgcag tgcagcggat tcagacgcgg
    21481 ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta cgtaccacgt
    21541 tagaaatggt ggacgctttc tggccgatag cgacatagat acatttgata ccggaatcgc
    21601 gctggttgat gatggcatcg atagccagtg cggttttacc tgtctgacgg tcaccgatga
    21661 tcaattcacg ctgaccacga ccgattggga tcatggagtc aacggcttta taaccggtct
    21721 gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct tctacagcag
    21781 agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga gtgttaacca
    21841 cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta cacttaactt
    21901 tcatgccttc ggcaaggtca gcgtacggac ccataacaac cgcacctaca gagtcgcgct
    21961 cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca ccctgcatac
    22021 aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata gtaccttcgt
    22081 tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt tcgctgattt
    22141 cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct gcaaggcgct
    22201 caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgatcggatg ataacgccag
    22261 ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga cgtttttcca
    22321 tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa atgacgtcta
    22381 cctcagcggt agcctcactc acggcacgaa ggtgaataaa ctgctccaga acatccggga
    22441 gcgcgttaag acgaccattt tcagccatta cccgaatcag gttctgaccg ttttcgtcca
    22501 gttgctcacc acaaactgcg ataaacgact cggcgagcgt ttctggcgca agcgcgccag
    22561 agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgtc agcatgtcct
    22621 gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc
    22681 gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag cgacaagttt
    22741 atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct tctcggcgcc
    22801 agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt tacgctcggc
    22861 ttcaatttct gcctgcgcct gggccacgat tttagtacgt tcctgttctg cctcagcttt
    22921 cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc tcgatgatta cctgggcttc
    22981 cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg
    23041 tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt cgatggctgc
    23101 cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa acgcgatggc
    23161 ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag ttaacgttct
    23221 gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc acgtacagac
    23281 ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata aagaactgag
    23341 tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat ttacccccga
    23401 ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca gcggcagcca
    23461 tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt cagttaaaac
    23521 gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac gatcgtcaga
    23581 accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca
    23641 ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa aatcagctca
    23701 ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa ctggtttgga cagcaggctt
    23761 accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc
    23821 aactctttcg tgaagccgcc gatgcctttc attttgatgt tgtagaacag aatcaggata
    23881 aatacgccca gtgccataga cagcgttacg ttcacgtccg cagacggaac cacacgcagt
    23941 gcaggcagac ccagtacatg ttcagcaatg tacggcagca agtcgatagg cagtaaatcc
    24001 atcagattca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc aatcagcttg
    24061 cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac cagctcaatc
    24121 gcggtctgaa acttacctgg cacaccgctg gtcgcctttt tggctacgct acggaataaa
    24181 accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt gattgtccag
    24241 aaggtggctg gggggttttg tggatccacc agcgagaatg tacgcaggtc cagctgaagg
    24301 ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc catgatgcct
    24361 tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc accaaaaccc
    24421 acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc accagtaaca
    24481 ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa tgtccaagcc acccggcctt
    24541 tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg ttaggcagaa
    24601 agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg aggctgaaca
    24661 gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga gcaacgtttc
    24721 gactcacgag cgacacagac atcacgtttt tcactcctgc tcccttcgag gtatgccgcg
    24781 tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt caaattatac
    24841 ggtgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg tttaaatatt
    24901 tttccagtgc atacaattgc gacttttccg ctacccctgt tcgatcatga aaaactgtaa
    24961 ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgcgc atgttcgcgc
    25021 acagcatatt tatttacttg gcaaatgatg cctttacaag tttatgatat ttcagtctaa
    25081 aaacagatac tgttttaata aataacattt acacaacaaa aaccactcat tgacattttt
    25141 aataatgttt taacagccaa tgatggttct tagcgccgat ttttagcaga ctgatatttt
    25201 cactaatgac ttattttctg cttaccaaaa aaagccacgt tatcttgttg atgcaaaaga
    25261 gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa aacctggttg ttgttaacag
    25321 tctaaccggt caatttttta tgattttttt gataaaaatt aaattttatt tgctttaatc
    25381 accaccagat gacgttcgcc atccagggct ggaacctgaa gtttaaccac tgattcgacc
    25441 tgatattctt cgggcaacaa agcgatttca tcttccggca tttgcccttt cagcgcgtag
    25501 aaacggcctt gctcaccagg aagatggtgg caccagctca ccatatcgtt cagagaggca
    25561 aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc taccctgctc
    25621 tgtactggtt caatattctc cagtttaagc tcatgttgca cctgacgaag gaaacgcacg
    25681 cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacgcacgat agagagtgga
    25741 atgcctggca gtcctggtcc ggtgccgaca tcgataaacc gttcaccttg cagatacggt
    25801 gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc gcggaccgaa
    25861 gtcaggttgt acgctttgtt ccatttatgc agcatattca cgtaggcaat aagctggttt
    25921 ttctggtgat cagtaagcga aatacctgcg tctttcagca gtaaggagag tttgttgagc
    25981 acggtgatta cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca ggcattttta
    26041 atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag cagaatggag
    26101 atggccgcag gcgtgacgcc agaaatacgc gaagcctggc cgatagaggc cggtttgtga
    26161 tcgttaagtt tggcgatcac ttcgttagaa agaccggata tctggcggta atccagtgtc
    26221 gcgggtagca gggtgttctc gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg
    26281 atataacctt cgtatttaac ctgaatctca acctgttccg ccgcctgttc gtctgtcaac
    26341 gcaggggcaa acggcgtcag cgtggttaat ttttcataag tcatttccgg acgacgcagc
    26401 agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt cacttcggct
    26461 gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc acgctcgata
    26521 ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga
    26581 ccgatttcag tcaaacgcag atccgcatta tcttcgcgca gcatcagacg atattctgcg
    26641 cgcgaagtaa acatacgata cggttctttg gttcctaaag tgcacaggtc atcaaccagt
    26701 acgccgagat acgcctgaga acgtgccgga gcccaacctt ctttgtcagc agacagacgg
    26761 gcagcgttaa gaccggccag caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg
    26821 ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt cggtttcagg
    26881 tcgcgaggat cgaagaagtc atactcaatg gcataacccg gacgcacgat cttcgcgttt
    26941 tccatcccct gcatagagcg gacgatttgc atctgcacat cgaacggcag gctggtggag
    27001 ataccgttcg gataaatttc attagaggtc agtccttccg gttcaaggaa gatctgatgc
    27061 tgatttctgt ctgcgaagcg catgactttg tcttcgatcg acgggcagta gcgtgggccg
    27121 acaccttcga tcacccctgc atacattggg ctacgatcga ggttactgcg gatcacatca
    27181 tgggttttct cgttggtatg agtgatataa cacggcacct gctggggatg cagggacgca
    27241 ttgcccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc cagcacgcta
    27301 aagtcgatgg ttcgagcatc aatacgcggc ggagtaccgg ttttcagacg accaacgcgc
    27361 agcggcagtt cacgcaaacg gcgagagagc ggaatggatg gcggatcgcc agcacggcca
    27421 ccgctgtaat tatccagacc gatatgaatt ttgccgtcga ggaacgtccc aacggtgagc
    27481 accacagctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac cacgcgatcg
    27541 ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcaggttcgg ttggttctcc
    27601 agcgccgtac gtaccgcctg acggtagcgc acacgatccg cctgagctcg ggtagcgcga
    27661 accgccggtc ctttgcttgc gtttagtatc ctaaactgga tacccgcttg atcgatcgct
    27721 ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt cccaataccg
    27781 ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt caaaagcaga
    27841 gtctgttgac ccatacgcgc cgcagccatc gcggcctcgg tgcctgcatg acccccgcca
    27901 atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca taacgcggta
    27961 tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgtcggct tgagaaagac
    28021 ctgggatcct gggtattaaa aagaagatct atttatttag agatctgttc tattgtgatc
    28081 tcttattagg atcgcactgc cctgtggata acaaggatcc gatttttaag atcaacaacc
    28141 tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc tgggatcaga
    28201 atgaggggtt atacataact caaaaactga acaacggttg ttctttggat agctaccggt
    28261 tgatccaagc ttcctgacag agttatccac agtagatcgc acgatctgta tacttatttg
    28321 agtaaattaa tccacgatcc cagccattct tctgccggat cttccggaat gtcgtgatca
    28381 agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt gagttctgcc
    28441 tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactgccaat accgattgtg
    28501 ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata gaaaggagaa
    28561 aggttgtccg gaatatctcc ggcaccgtgg gtggagctga taaccagcca gatccccgag
    28621 gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc cacctcttcc
    28681 agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct gccgctgata
    28741 agagtgatat ctgccataaa ccgccacctt tattaagagt ggcgtattgt acgctgtgaa
    28801 cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgatcag ggcttgatgg
    28861 tacgcatgat cgggttttgc aggacgatca atgtctcggt ggactgaatt tcatcaattg
    28921 tttggatctt gttgataagt acatgctgga gagcgtcgat cgaacggcac ataactttta
    28981 taaagatgct gtagtggccg gttgtgtaat aggcttcagt gacttcatca aggctttcca
    29041 gctttgccag cgcggaaggg tagtctttgg cgctctttaa tataatgccg ataaagcagc
    29101 ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta atgatccccg
    29161 cctgcttcat tttctctact cgaacgtgaa tcgtccccgg actgacgcca aattgtttcg
    29221 ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg ccacggtcca
    29281 gattgtcgat cagataattt tccataggat tttcttatgc agattgatga ttcattctat
    29341 tttagccgtc ttttttaatg aatcaaaagt gtgttaggct ttttattgaa tgattattgc
    29401 atgtgtgtcg gtttttgttg cttaatcata agcaacagga cgcaggagta taaaaaatga
    29461 aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac ttttctcgcc
    29521 aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc cgtgtggggg
    29581 atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa gtgaaagctc
    29641 tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt cagaccttag
    29701 ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc cttcgccccg
    29761 atgaagaccg tctttctccg ttgcactcgg tctatgttga ccagtgggac tgggaacgcg
    29821 taatgggcga cggtgagcgt caactctcga ctctgaaaag cacggtagag gcgatctggg
    29881 cgggaattaa agcaaccgaa gctgcggtta acgaagagtt tggcctggca ccgttcctgc
    29941 cggatcagat tcacttcgta cacagccagg agttactgtc tcgttatccg gatctcgatg
    30001 ccaaagggcg tgagcgggcg attgcgaaag atcttggcgc ggtattcctc gtcgggattg
    30061 gcggcaagtt gagtgatggt catcgtcacg acgtgcgcgc accggattat gatgactgga
    30121 gcaccccgtc agagctgggc catgcgggcc tgaacggcga tattctggtg tggaacccgg
    30181 tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc gacacgctga
    30241 agcatcagct ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg catcaggcgc
    30301 tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct cgtttgacta
    30361 tgctgctgct gcaactgccg catatcggcc aggttcagtg tggagtatgg ccagctgctg
    30421 ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc tgagcaggcg
    30481 gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga tgccgggttt
    30541 gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct gcagctcttt
    30601 cactttactc gtcacgtcgt caggcaaccg ctgagcgata aaatcagaaa tcaccaccgc
    30661 atcggcatca aaccattccc tgctttgcaa gcgttccata atggcgcgaa aacaactggc
    30721 aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt cgatgccttg
    30781 tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat aacagcgccg
    30841 gttttcagcg agagcaatgc gcatcaaagc caggcagaac gctttcgcac actgttcatt
    30901 aaagccgccc attgagccgg aagtatccac acagacaata aacggcccgc gcggctgttc
    30961 atcgtaatct ttatgtacca ccggacgttc gaacactttt tcacgccacg actcaccgtg
    31021 caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat actccagttc
    31081 cgttatccct agtgtcgcca gttctggcgg caggagacgt aaaatatcat cgctttgttg
    31141 cagaccatca acctgctcag gaaccgtcgc cggttcgcgc accatggtgc ggaaggtttc
    31201 catctgcgca tcgttgcgcg gtattgattt ggcttcccga gaacgcccca gctgctctgc
    31261 caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa taaactgata
    31321 gtcgccacgt ttaagttgac cggcgctcat atcccacaga cgaccagctg cggtattgtt
    31381 atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa cttcacttaa
    31441 cagttgttcg cgttcttctt ctaatagctg ttgatttaac gtcgttgctt gcacgatcag
    31501 acttaaacgc caacgctgga gaaaaagcgt gtgtaacgct gaagttattg tgctgttagc
    31561 atcaaccaac tgccgggctt gttctgccca tggggaattc agacgatgca gtaagtccag
    31621 gatctgtggt agctgcacaa taaactgcgg cgtggagagg agctggcttt gctgatagca
    31681 catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgac tgcgcagcgc
    31741 ctcacgccag cggggaacat catcagtgat tgccgccttc agtcgtggga atttttcaaa
    31801 gaagaccgcc agctgcggtg aggccagcag tgcgatgatc atctcttcga tcaatccctc
    31861 ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg tgcctgacga
    31921 atctgtgcac cgacatcctg taggctgtct tcaatgcgac ctaaccagtc gccagggata
    31981 aacagacatt tttgctgttc gctgaataaa gcgtgttgct tacgccagtc gctttcaagc
    32041 tcttccagtt gttgttttat ttcacctggc agaccttcag ccgatgaacc gggaagtgcc
    32101 agcgtactgc cttgtaaact tacatcgcgt acaacaagat gttgggtgct atcaacttcc
    32161 agattcagtt tctgggcaaa gccgataccg ttcagtttgc cgcgaatttc accaccttta
    32221 ctcagccact gttccagcgc gctacgctca aaggagatat gaaccacttc catatcatgc
    32281 agttttaacg gtttttgcag cagcagagtc agagtggaag cagtaacgtt aacaggaagt
    32341 tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaacgttaa ggctgtttta
    32401 tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgcccag gcgggtcaac
    32461 atcccttgct gttgccaggc atgaccggtc atcaatacat caatttgttg ttgtatcaaa
    32521 ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc aaccggggca
    32581 acagcactgc gaccgctaaa aaaggcgctg gcctgcaata agcggatcgc ttttttccag
    32641 cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg cagaataaaa
    32701 atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg ccagcgttca
    32761 tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc atcctgttga
    32821 ctggtcagca tggagcggaa attcgcttta tcttgcactt tatctaacca tagacgaatc
    32881 agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc gttggaggcc
    32941 gccaccagta gacgcatcgg gattttttct acgtgtgcac cgttgcggaa ctggcgctcg
    33001 ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat ctcatccaga
    33061 aagacgattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg cccttcatct
    33121 tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc
    33181 agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc gatcaaactt
    33241 ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc cgctaataaa
    33301 cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact gctcaggcgg
    33361 gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc tttcgtcaaa
    33421 agttctgcgt aaattgcgag tatagacgtt tcttgctggt gactaaaata gtctcaaagg
    33481 ggggtaatac cgatcagtta aggatcagtt gaccgatcca gtggctgtg
//
LOCUS       DAZSNG010000399         9713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.136, whole genome shotgun sequence.
ACCESSION   DAZSNG010000399 DAZSNG010000000
VERSION     DAZSNG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.136"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..205)
                     /locus_tag="ACHW4Z_004591"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACHW4Z_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHW4Z_004592"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHW4Z_004592"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR8883870.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACHW4Z_004593"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACHW4Z_004593"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8883871.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACHW4Z_004594"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACHW4Z_004594"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8883872.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACHW4Z_004595"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACHW4Z_004595"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8883873.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACHW4Z_004596"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACHW4Z_004596"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8883874.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6233
                     /locus_tag="ACHW4Z_004597"
     CDS             5100..6233
                     /locus_tag="ACHW4Z_004597"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR8883875.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /gene="blc"
                     /locus_tag="ACHW4Z_004598"
     CDS             6322..6855
                     /gene="blc"
                     /locus_tag="ACHW4Z_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8883876.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACHW4Z_004599"
     CDS             complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACHW4Z_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8883877.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="ACHW4Z_004600"
     CDS             complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="ACHW4Z_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8883878.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="ACHW4Z_004601"
     CDS             complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="ACHW4Z_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8883879.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7779..8345)
                     /gene="efp"
                     /locus_tag="ACHW4Z_004602"
     CDS             complement(7779..8345)
                     /gene="efp"
                     /locus_tag="ACHW4Z_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8883880.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8387..9415
                     /gene="epmB"
                     /locus_tag="ACHW4Z_004603"
     CDS             8387..9415
                     /gene="epmB"
                     /locus_tag="ACHW4Z_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR8883881.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2485 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat
     6421 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     6541 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
     7081 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg
     7321 acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc
     7381 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
     7441 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
     7501 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
     7561 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag
     7621 atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg
     7681 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
     7741 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
     7801 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
     7861 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
     7921 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
     7981 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg
     8041 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
     8101 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
     8161 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
     8221 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
     8281 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
     8341 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
     8401 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
     8461 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
     8521 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
     8581 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
     8641 cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
     8701 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
     8761 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
     8821 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
     8881 cgatccgctg atggcgaaag agcacgagct ggactggttg ctcacacaac tggaagccat
     8941 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
     9001 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
     9061 catcaatcat gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg
     9121 tgtcggtgtc accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
     9181 aacgttggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca
     9241 tgtgctagat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
     9301 gattatgcgt gagttgctga cattggtgtc gggatatctg gtgccgaaac tggcgcgaga
     9361 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
     9421 taaacaggga agttaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
     9481 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
     9541 tccggcaaga gcaacaaacg caagtacagc aaccactgag aaatttaatc tttgcacgtt
     9601 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     9661 taattaacct ttcttttcgg taatgactcc aacttactga tagtgtttta tgt
//
LOCUS       DAZSNG010000400         8962 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.148, whole genome shotgun sequence.
ACCESSION   DAZSNG010000400 DAZSNG010000000
VERSION     DAZSNG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.148"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(187..537)
                     /locus_tag="ACHW4Z_004604"
                     /pseudo
     CDS             complement(187..537)
                     /locus_tag="ACHW4Z_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(740..1753)
                     /gene="intI1"
                     /locus_tag="ACHW4Z_004605"
     CDS             complement(740..1753)
                     /gene="intI1"
                     /locus_tag="ACHW4Z_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8883882.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1911..2384
                     /gene="dfrA17"
                     /locus_tag="ACHW4Z_004606"
     CDS             1911..2384
                     /gene="dfrA17"
                     /locus_tag="ACHW4Z_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8883883.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2515..3303
                     /locus_tag="ACHW4Z_004607"
     CDS             2515..3303
                     /locus_tag="ACHW4Z_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR8883884.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3509..3856
                     /locus_tag="ACHW4Z_004608"
     CDS             3509..3856
                     /locus_tag="ACHW4Z_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8883885.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            3850..4689
                     /gene="sul1"
                     /locus_tag="ACHW4Z_004609"
     CDS             3850..4689
                     /gene="sul1"
                     /locus_tag="ACHW4Z_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR8883886.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            4817..5020
                     /locus_tag="ACHW4Z_004610"
     CDS             4817..5020
                     /locus_tag="ACHW4Z_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8883887.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5176..6381
                     /gene="chrA"
                     /locus_tag="ACHW4Z_004611"
     CDS             5176..6381
                     /gene="chrA"
                     /locus_tag="ACHW4Z_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR8883888.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6392..6697
                     /locus_tag="ACHW4Z_004612"
     CDS             6392..6697
                     /locus_tag="ACHW4Z_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR8883889.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            6924..7688
                     /locus_tag="ACHW4Z_004613"
     CDS             6924..7688
                     /locus_tag="ACHW4Z_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR8883890.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8181..8765)
                     /gene="mphR(A)"
                     /locus_tag="ACHW4Z_004614"
     CDS             complement(8181..8765)
                     /gene="mphR(A)"
                     /locus_tag="ACHW4Z_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR8883891.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(8765..>8962)
                     /locus_tag="ACHW4Z_004615"
     CDS             complement(8765..>8962)
                     /locus_tag="ACHW4Z_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8883892.1"
                     /translation="AVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRL
                     QFATMAAAATASAPTHTEGEH"
BASE COUNT         1875 a         2516 c         2589 g         1982 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc cccaatgcgc tgtccagtca
       61 tggaattccc tgcaaaatgt cagggaagac tctatgacct tcaacgagat atgtcaataa
      121 attcaaaatt caatcctatc ctgacgcaat ttacacatgg catctgacat caggttaggg
      181 tatgcctcaa cctgacggcg gcgaatcaca agcgtccggt ttgacgctgg tttcggtcgc
      241 agtgctggcc cgcgcctgga agcgctgata gcactccagc ccgcagaaat gctccacgta
      301 ctcggcccct tccggcgtga aggcggcatc gagcgggatt tccttgcagc acacgcagca
      361 actggtgctc ggttcattgg cgttcatggt ggtgttcctc cattggttga cgaagccgac
      421 gaaggccgcc gccggcatcg gcctggcgaa caggaaaccc tgcaccgtgt cgcaacccgc
      481 ctgccgcaac cacgcaaggc agtcgggtgt ttccacaccc tcggctacca cctccattgt
      541 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
      601 gttggatcca tcaggcaacg acgggctgct gccggccatc agcggacgca gggaggactt
      661 tccgcaaccg gccgttcgat gcggcaccga tggccttcgc gcaggggtag tgaatccgcc
      721 aggattgact tgcgctgccc tacctctcac tagtgagggg cggcagcgca tcaagcggtg
      781 agcgcactcc ggcaccgcca actttcagca catgcgtgta aatcatcgtc gtagagacgt
      841 cggaatggcc gagcagatcc tgcacggttc gaatgtcgta accgctgcgg agcaaggccg
      901 tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt cgtgatgcct gcttgttcta
      961 cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat gtgatggcga cgcacgacac
     1021 cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac ccagaaccac ggccaggaat
     1081 gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg ctgcggccct
     1141 cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc aggctgggtg
     1201 ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc cgcacgatga
     1261 tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca ctgatccgca
     1321 tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga aaaccgagga
     1381 tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc
     1441 cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg
     1501 ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
     1561 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
     1621 tgaaggcacg aacccagtgg acataagcct gttcggttcg taaactgtaa tgcaagtagc
     1681 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1741 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
     1801 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1861 cgcagcaggg cagtcgccct aaaacaaagt tagccattaa gggagttaaa ttgaaaatat
     1921 cattgatttc tgcagtgtca gaaaatggcg taatcggtag tggtcctgat atcccgtggt
     1981 cagtaaaagg tgagcaacta ctctttaaag cgctcacata taatcaatgg ctccttgtcg
     2041 gaagaaaaac atttgactct atgggtgttc ttccaaatcg caaatatgca gtagtgtcaa
     2101 agaacggaat ttcaagctca aatgaaaacg tcctagtttt tccttcaata gaaaatgctt
     2161 tgaaagagct atcaaaagtt acagatcatg tatatgtctc tggcgggggt caaatctata
     2221 atagccttat tgaaaaagca gatataattc atttgtctac tgttcacgtt gaagtcgaag
     2281 gtgatatcaa attccctata atgcctgaga atttcaattt ggtttttgaa cagtttttta
     2341 tgtctaatat aaattataca taccagattt ggaaaaaagg ctaacaatgc gttgcagcac
     2401 cagtcgcttc gctccttgga cagcttttaa gtcgcgtctt tgtggttttg ctgcgcaaaa
     2461 gtattccaca aagccgcaac ttaaaagctg ccgctgaact taacgttagg catcatgggt
     2521 gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg ctcgcgcggt gatcgagcgc
     2581 catctggctg cgacactgga cacaatccac ctgttcggat ctgcgatcga tggagggctg
     2641 aagccggaca gcgacataga cttgctcgtg accgtcagcg ccgcacctaa cgattcgctc
     2701 cggcaggcgc taatgctcga tttgctgaaa gtctcatcac cgccaggcga tggcggaaca
     2761 tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag tagtgccttg gcgctatccg
     2821 gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg acatcctttc cggaacgttc
     2881 gagcctgccg ttctggatca cgatcttgcg attttgctga ccaaggcgag gcaacacagc
     2941 cttgcgcttc taggcccatc cgcagccacg tttttcgagc cggtgccgaa ggagcatttc
     3001 tccaaggcgc ttttcgacac tattgcccag tggaatgcag agtcggattg gaagggtgac
     3061 gagcggaacg tcgttcttgc tcttgctcgc atttggtaca gcgcttcaac tggtctcatt
     3121 gctcctaagg acgttgctgc cgcatgggta tcggagcgtt tgcctgccga gcatcggccc
     3181 ctcatctgca aggcacgcgc ggcgtacctg ggtagcgagg acgacgacct agcaatgcgc
     3241 gtcgaagaga cggccgcgtt cgttcgatat gccaaagcaa cgattgagag aatcttgcgt
     3301 tgagcggcat gtgcgaaaag tgcatcgacc cgcgccgagg gcatctgatg cctaactcgg
     3361 cgttcaagcg gacgggctgc gcccgccgct caactatgcg ttagatgcac taagcacata
     3421 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     3481 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat
     3541 agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta ctaagcttgc
     3601 cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt ctctggttct
     3661 gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg tcgtcataat
     3721 tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct ttgtaggtat
     3781 ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt cgctgcggag
     3841 gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgaggactc cttcttcgat
     3901 gagagccggc ggctagaccc cgccggcgct gtcaccgcgg cgatcgaaat gctgcgagtc
     3961 ggatcagacg tcgtggatgt cggaccggcc gccagccatc cggacgcgag gcctgtatcg
     4021 ccggccgatg agatcagacg tattgcgccg ctcttagacg ccctgtccga tcagatgcac
     4081 cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct atgcgctcaa gcgcggcgtg
     4141 ggctacctga acgatatcca aggatttcct gaccctgcgc tctatcccga tattgctgag
     4201 gcggactgca ggctggtggt tatgcactca gcgcagcggg atggcatcgc cacccgcacc
     4261 ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc ggttcttcga ggcgcgggtt
     4321 tccgccttgc gacggagcgg ggtcgctgcc gaccggctca tcctcgatcc ggggatggga
     4381 tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc tgtcgaacct tcaaaagctg
     4441 aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc ggaaatcctt cttgggcgcc
     4501 accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc ttgcggcgga acttcacgcg
     4561 atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg gagatctgcg aagcgcaatc
     4621 accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg ccagagaccg agggttagat
     4681 catgcctagc attcaccttc cggccgcccg ctagcggacc ctggtcaggt tccgcgaagg
     4741 tgggcgcaga catgctgggc tcgtcaggat caaactgcac tatgaggcgg cggttcatac
     4801 cgcgccaggg gagcgaatgg acagcgagga gcctccgaac gttcgggtcg cctgctcggg
     4861 tgatatcgac gaggttgtgc ggctgatgca cgacgctgcg gcgtggatgt ccgccaaggg
     4921 aacgcccgcc tgggacgtcg cgcggatcga ccggacattc gcggagacct tcgtcctgag
     4981 atccgagctc ctagggatcg cctcagaaaa cggaaaataa agcacgctaa gccgtaagta
     5041 agcgtgctcc tgtgaaagcc acagctaaaa ctgcgtagta cacatagagg tatttttttc
     5101 tagatttata tcgaatttcg ttatcgaatt tcgcttatta ttgatcaact cgttgcagat
     5161 catgaaggcg aaagtatgac gaataaccct accgatgaca gcagaccatg gtcggtcttt
     5221 cttatttttc tgcggcttgg attgacatct tttggcggcc ccattgcgca cttgggctac
     5281 ttccgcgccg aatttgtcac acggcggcgc tggctctccg aacggagcta tgctgacttg
     5341 gtcgcgcttt gtcagttctt gccagggcct gcaagcagcc aggtcggcat agcggtagga
     5401 ctgtctcggg ctggatacag cggggcgctg gctgcttggg ctggcttcac gctgccgtct
     5461 gccatagcct tgatcctttt tgcgctcggc atctccagct atggcgatta cgtctcgcag
     5521 ggcgcgttgc atggcttaaa agtggtggct gtggccgtgg tcgctcaagc agtatggggc
     5581 atggcgcgta acctatgcac ggatgggctg cgagtcacca tcatggcaat tgctacctgc
     5641 gtcgttttac ttgtgccgtc cgcgtgggga caggttggcg tgattgctat cgcaggcatc
     5701 gcaggccggt tattgttcaa gccagcgaaa gttgttgagc atgaccccct acctatcacg
     5761 gtcagtcacc gggccggcgt gctttggctc tcgctgttct ttgtcttgct gattggcctg
     5821 ccggtgttgg ccgaactgat gccaagtcaa accatggcaa tggtggattc cttctatcgt
     5881 gtcggatcac tggtgttcgg cggtggtcac gttgtgctgc cattactgca agccgaagtg
     5941 gtgccctccg gctgggtcaa caatgaatcc tttctcgcgg ggtacggggc agctcaagcg
     6001 gtgcccggcc ctttgttcac gttcgccgcg tttcttggtg cctcgatgaa caccgccccg
     6061 tcgggctgga tcggcggcat tgtgtgtctg ctggctatct tcgcgccctc gttcttgctg
     6121 gtcgtcggat caatgccatt ttgggagcgt ttgcgccgca atacaggcat ccaagctgcg
     6181 ctggccggga tcaatgccgc tgtagtcggc ttgctgctgg ccgcgctgta tcagcctgta
     6241 tggactagcg ccatctttca gccgcaagac ttcggcttgg cattagttgc ccttgtcgca
     6301 cttatgttct ggaagctccc gccgtggctg gttgtcctcg gtagcggtgc agctgggtgg
     6361 ctgttgagcg tcgctctgtg aggcacaaaa aatgactgac aaagacctct acggcggttt
     6421 gatccgcctg cacatccttc accatgcagc cgaggaacct gtctttgggc tggggatcat
     6481 cgaagagcta cgccgacacg gctacgagat gagcgctggc accgtgtacc cgatgctgca
     6541 cggcctggaa aaaaaaggct atctgacctc acgccacgaa cgcaccgggc gacgtgaacg
     6601 gcgtgtttat gacatcactg aacaaggcag aactgcactt gctgatgcga aaacaaaggt
     6661 caaggagctg ttcggagagt tggtagaagg tggttgagtt tttgcttcta tgtcaggttg
     6721 agctataccc taactggatt gtcattttca gaagacgact gcaccagttg attgggcgta
     6781 atggctgttg tgcagccagc tcctgacagt tcaatatcag aagtgatctg caccaatctc
     6841 gactatgctc aatactcgtg tgggctctgt tgcaaaaatc gtgaagcttg agcatgcttg
     6901 gcggagattg gacggacgga acgatgacgg atttcaagtg gcgccatttc cagggtgatg
     6961 tgatcctgtg ggcggtgcgc tggtattgtc gctatccgat cagctatcgc gaccttgagg
     7021 aaatgctggc ggaacgcggc atttcggtcg accatacgac gatctatcgc tgggtccagt
     7081 gctacgcccc ggagatggag aagcggctgc gctggttctg gcggcgtggc tttgatccga
     7141 gctggcgcct ggatgaaacc tacgtcaagg tgcggggcaa gtggacctac ctgtaccggg
     7201 cagtcgacaa gcggggcgac acgatcgatt tctacctgtc gccgacccgc agcgccaagg
     7261 cagcgaagcg gttcctgggc aaggccctgc gaggcctgaa gcactgggaa aagcctgcca
     7321 cgctcaatac cgacaaagcg ccgagctatg gtgcagcgat caccgaattg aagcgcgaag
     7381 gaaagctgga ccgggagacg gcccaccggc aggtgaagta tctcaataac gtgatcgagg
     7441 ccgatcacgg aaagctcaag atactgatca agccggtgcg cggtttcaaa tcgatcccca
     7501 cggcctatgc cacgatcaag ggattcgaag tcatgcgagc cctgcgcaaa ggacaggctc
     7561 gcccctggtg cctgcagccc ggcatcaggg gcgaggtgcg ccttgtggag agagcttttg
     7621 gcattgggcc ctcggcgctg acggaggcca tgggcatgct caaccaccat ttcgcagcag
     7681 ccgcctgatc ggcgcagagc gacagcctac ctctgactgc cgccaatctt tgcaacagag
     7741 cccgccgtgc tagtctgctc ggtgatggtg gagtgaagcc aacccgcaat cgggttatga
     7801 atctgcatcg cgattcgcaa tcagctgtct cttgagcatg tcgaactcct gcgatgttat
     7861 ctcgccgtgc tcgtgctgct acaccaactt gtccaactca tccgccacac cgctccccac
     7921 cacctgcgat ggccaggttg tttgctgggc gcattggcgg agtatcgtct cgacccggga
     7981 cacccacacc ggcacaacct tacgggagtg attcactgtc aaagaatcgg cccggtgctc
     8041 tgacgcaagt atcgggatgg tcaccatttg taagccgtag acctgagtgg tgatcaagac
     8101 ttcgatacca ccgaccgtac cggtactaat cgacgacggt cgtattcgtc gcctgccgca
     8161 gggactctgc acacctccgt ttacgcatgt gcctggagga gttggaaatc gtcgtgttcg
     8221 ggaaacatta aacacaggat ggcagcgatc tgagccagca catgatcagc tagctcacca
     8281 tccggatcga cggcccactg catcgtcgcg ccagcgatga ccgagtgcag gagcaactca
     8341 gctgccgcag gagcacctgg gggcagtcgc ttgcggatcc cctccaccac cgcgcggttc
     8401 cgctggatcg caagcgtgcg tagctccggc acctggagct cgtaccagga gatgagatag
     8461 ttcaccgaga agtcgttgcg agtgttcatg ctccgaacga gcacctgcaa aaattcccag
     8521 agcccttgcg gccctgcgcc tatcggtatc gcattcaggt aatgccgcac ctgctcgacg
     8581 ccgcgctcca tcatcctcac cagcagcgta tcgcggttgg tgaagcgctg gattaacgct
     8641 gcgcgggaga gccccacctc ctttgctact ccgctgagcg tgaactctat gggaccgcaa
     8701 cgcttcagca ctacggtggc ggcctcgagt acctcgtcat cggacttgag cttggggcgg
     8761 ggcatcagtg ttcaccttct gtatgggttg gggcggaggc tgtggctgcc gccgccattg
     8821 tagcaaattg aagacggagc gagagtagag ccacgagccc cgcaaacacg gccgatacaa
     8881 cgaggccagg gagcggacca gcaaggtcga caaacgcgcc ggccgcaagc ataaccatgg
     8941 gcgaggctga cagcatcacc gc
//
LOCUS       DAZSNG010000401         7053 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.168, whole genome shotgun sequence.
ACCESSION   DAZSNG010000401 DAZSNG010000000
VERSION     DAZSNG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.168"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            59..1102
                     /locus_tag="ACHW4Z_004616"
     CDS             59..1102
                     /locus_tag="ACHW4Z_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR8883893.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHW4Z_004617"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHW4Z_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHW4Z_004618"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHW4Z_004618"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR8883894.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHW4Z_004619"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHW4Z_004619"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8883895.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHW4Z_004620"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHW4Z_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8883896.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHW4Z_004621"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHW4Z_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8883897.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHW4Z_004622"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHW4Z_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8883898.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DAZSNG010000402         2051 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.276, whole genome shotgun sequence.
ACCESSION   DAZSNG010000402 DAZSNG010000000
VERSION     DAZSNG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.276"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1015
                     /locus_tag="ACHW4Z_004623"
     CDS             110..1015
                     /locus_tag="ACHW4Z_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR8883899.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..>2051
                     /gene="mrx(A)"
                     /locus_tag="ACHW4Z_004624"
                     /gene_synonym="mrx"
     CDS             1012..>2051
                     /gene="mrx(A)"
                     /locus_tag="ACHW4Z_004624"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR8883900.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVS"
BASE COUNT          307 a          658 c          669 g          417 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct c
//
LOCUS       DAZSNG010000403         1817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.293, whole genome shotgun sequence.
ACCESSION   DAZSNG010000403 DAZSNG010000000
VERSION     DAZSNG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.293"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            112..207
                     /locus_tag="ACHW4Z_004625"
     CDS             112..207
                     /locus_tag="ACHW4Z_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR8883901.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHW4Z_004626"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHW4Z_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR8883902.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          303 c          297 g          554 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atggtgt
//
LOCUS       DAZSNG010000404         1734 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 18-00PHL-SF35
            SAMN39618770-rid23473143.guided.302, whole genome shotgun sequence.
ACCESSION   DAZSNG010000404 DAZSNG010000000
VERSION     DAZSNG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618770
            Sequence Read Archive: SRR27734402
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394714.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394714.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:39:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,629
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 469
            CDSs (without protein)            :: 469
            Pseudo Genes (ambiguous residues) :: 0 of 469
            Pseudo Genes (frameshifted)       :: 216 of 469
            Pseudo Genes (incomplete)         :: 159 of 469
            Pseudo Genes (internal stop)      :: 166 of 469
            Pseudo Genes (multiple problems)  :: 67 of 469
            Pseudo Genes (short protein)      :: 1 of 469
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618770-rid23473143.guided.302"
                     /isolate="18-00PHL-SF35"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..474
                     /locus_tag="ACHW4Z_004627"
     CDS             <1..474
                     /locus_tag="ACHW4Z_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8883903.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="ACHW4Z_004628"
     CDS             657..1517
                     /locus_tag="ACHW4Z_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR8883904.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1667..1734)
                     /locus_tag="ACHW4Z_004629"
                     /pseudo
     CDS             complement(1667..1734)
                     /locus_tag="ACHW4Z_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          462 a          386 c          443 g          443 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaac
//
